阅读说明：本技术 一种提高冬种紫甘薯可溶性糖含量的种植方法 (Planting method for increasing soluble sugar content of winter purple sweet potatoes ) 是由 后猛 唐秀桦 李强 韦民政 张允刚 熊军 于 2021-08-11 设计创作，主要内容包括：本发明涉及酵素应用发酵技术领域,特别涉及一种提高冬种紫甘薯可溶性糖含量的种植方法,本发明通过对不同原料的酵素进行前期喷施实验,得出了一组能提高紫甘薯可溶性糖含量的酵素原料组方及其制备方法,然后通过厨余原料组方优化配比,得出了适合紫甘薯生长的酵素原料组方配比和制备方法,在对厨余垃圾进行简单处理即可制备得到高效的酵素,将该酵素喷施在紫甘薯中,在特定的施用方法和条件下达到提高紫甘薯桂经薯8号可溶性糖含量的目的,整体方法简便易行,同时有效的对厨余垃圾进行利用,提高了对厨余垃圾的利用能力。(The invention relates to the technical field of enzyme application fermentation, in particular to a planting method for improving the soluble sugar content of a winter purple sweet potato, a group of enzyme raw material formulas capable of improving the soluble sugar content of the purple sweet potato and a preparation method thereof are obtained by carrying out early-stage spraying experiments on enzymes of different raw materials, then the formula ratio and the preparation method of the enzyme raw materials suitable for the growth of the purple sweet potato are obtained by optimizing the ratio of the kitchen waste raw material formulas, efficient enzyme can be prepared by simply treating kitchen waste, the enzyme is sprayed in the purple sweet potato, the purpose of improving the soluble sugar content of the purple sweet potato Ipomoea batatas No. 8 is achieved under a specific application method and conditions, the integral method is simple and easy to implement, the kitchen waste is effectively utilized, and the utilization capacity of the kitchen waste is improved.)

1. The ferment for improving the content of the soluble sugar of the winter purple sweet potatoes is characterized by being prepared by mixing kitchen waste slurry, sugar and water according to the mass ratio of 4:1:10 to obtain kitchen waste juice, adding sour juice accounting for 5-10% of the mass of the kitchen waste juice, uniformly mixing and fermenting; the kitchen waste slurry is composed of any 5 raw materials of rice, vegetable waste, apple waste, pear waste, watermelon waste and/or cucumber waste; the sour fruit juice comprises passion fruit juice and/or lemon juice.

2. The enzyme for increasing the content of soluble sugar in winter purple sweet potatoes according to claim 1, wherein the kitchen waste is prepared by mixing rice, vegetable waste, apple waste, pear waste, watermelon waste and cucumber waste in a mass ratio of 1-5:1-5:1-5, and the sour fruit juice is passion fruit juice.

3. The ferment for improving the content of the soluble sugar of the winter purple sweet potato as claimed in claim 1, wherein the kitchen waste is prepared by mixing rice, vegetable waste, apple waste, pear waste, watermelon waste and cucumber waste according to a mass ratio of 2:1:2:3:4, and the sour fruit juice is passion fruit juice accounting for 10% of the total mixture.

4. A process for preparing the ferment of claims 1 to 3, comprising the steps of:

(1) mixing and chopping kitchen waste, crushing the kitchen waste by using a stirrer to obtain kitchen waste slurry, mixing the kitchen waste slurry, sugar and water according to the mass ratio of 4:1:10, and putting the mixture into a plastic barrel to be uniformly mixed to obtain kitchen waste juice;

(2) adding Passion fruit juice 10% of the kitchen waste juice, mixing, and keeping the charging amount at 80% of the whole container; fermenting under the conditions of light-proof and room temperature, opening the container every day to deflate during fermentation, stirring the materials floating on the liquid surface into the liquid, repeating the operation, and standing for 3 months under the condition of light-proof without deflating after one month.

5. A planting method for increasing the content of soluble sugar in a winter purple sweet potato by using the ferment of claims 1 to 3 and/or the ferment prepared by the method of claim 4, wherein the planting method comprises the following steps: diluting 10ml of enzyme stock solution by 2000 times at the 80 th day after the purple sweet potato seedlings are planted and spraying the purple sweet potato seedlings; then spraying 1 time every 7d, and spraying 3 times in total.

[ technical field ] A method for producing a semiconductor device

The invention relates to the technical field of ferment application fermentation, and particularly relates to a planting method for improving the content of soluble sugar in a winter purple sweet potato.

[ background of the invention ]

The ferment is a product containing specific biological activity, which is prepared by fermenting animals, plants, fungi and the like serving as raw materials through microorganisms. The components of the ferment on the market are complex, and the ferment not only refers to 'enzyme', but also comprises polysaccharides, organic acids, alcohol, pectin, protein, vitamins, minerals and hundreds of substances generated after fermentation. The raw material sources for producing the ferment are wide: organic matters such as animals, plants and the like can become raw materials of the enzyme, and the enzyme has wide application, can be used for preparing health-care products by food processing, and can also be used for preparing components such as organic fertilizers or additives.

In recent years, due to environmental protection requirements, preparation of enzymes from kitchen waste has become an effective way for development of enzyme industry, and the use of enzymes as fertilizer or additive added to plant growth has been studied more maturely, and reports of the prior art that the soluble sugar content of some plants is increased by using enzymes have been reported, however, in the practical application process, those skilled in the art find that: the ferment of different raw materials, the material that produces after its fermentation is different, because the composition is complicated, and the analysis expense is high, and the ferment of using on the plant is its composition of little analysis, consequently, in the in-process of using, hardly has the associativity between the different ferment, and in the application process in fact, the skilled person also discovers that the ferment of different raw materials, the effect that plays is also inconsistent on different plants.

The purple sweet potato is one of sweet potatoes, is named because the skin and the meat of the sweet potato are purple, contains rich anthocyanin, is popular among consumers for resisting cancers, but is not sweet generally, has low sugar content, particularly low soluble sugar content, is not as good as the taste of yellow sweet potatoes, is low in soluble sugar content, is related to varieties on one hand, and is also related to planting management on the other hand, at present, in order to adjust the production period of the purple sweet potatoes, planting conditions are improved, a southern planting area selects to plant the purple sweet potatoes in winter (namely 10 months to 3 months next year), and the sweet potatoes planted in winter are generally lower in soluble sugar content than normal seasons due to the fact that the illumination temperature conditions are different from spring and summer; the taste of the purple sweet potatoes in winter is inferior to that of the purple sweet potatoes in summer and autumn, and the quality is influenced; therefore, the demand for improving the content of the soluble sugar in the purple sweet potatoes in winter is a technical problem to be solved by the purple sweet potatoes in winter at present. In the prior art, the application of enzyme to other crops to improve the content of soluble sugar in crops is reported, but through research, the application of the enzyme and the application method to other plants are not suitable for sweet potatoes, because different crops have different metabolic capacities, different planting seasons and different climates, so that the absorption capacities of the crops to related nutritional ingredients are different, and finally the content of the soluble sugar is different.

[ summary of the invention ]

In view of the above, in order to improve the taste and soluble sugar content of purple sweet potatoes, the application realizes the purpose of improving the soluble sugar content of purple sweet potatoes through planting management by researching different raw material enzymes and different application methods.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the ferment for improving the content of the soluble sugar of the winter purple sweet potatoes is prepared by mixing kitchen waste slurry, sugar and water according to the mass ratio of 4:1:10 to obtain kitchen waste juice, adding sour fruit juice accounting for 5-10% of the mass of the kitchen waste juice, uniformly mixing and fermenting; the kitchen waste slurry is composed of any 5 raw materials of rice, vegetable waste, apple waste, pear waste, watermelon waste and/or cucumber waste; the sour fruit juice comprises passion fruit juice and/or lemon juice.

Further, the kitchen waste is prepared by mixing rice, vegetable waste, apple waste, pear waste, watermelon waste and cucumber waste according to a mass ratio of 1-5:1-5:1-5, and the sour fruit juice is passion fruit juice.

Further, the kitchen waste is prepared by mixing white rice, vegetable waste, apple waste, pear waste, watermelon waste and cucumber waste according to a mass ratio of 2:1:2:3:4, and the sour fruit juice is passion fruit juice accounting for 10% of the total mixture.

The invention also comprises a method for preparing the ferment, which comprises the following steps:

(1) mixing and chopping kitchen waste, crushing the kitchen waste by using a stirrer to obtain kitchen waste slurry, mixing the kitchen waste slurry, sugar and water according to the mass ratio of 4:1:10, and putting the mixture into a plastic barrel to be uniformly mixed to obtain kitchen waste juice;

(2) adding Passion fruit juice 10% of the kitchen waste juice, mixing, and keeping the charging amount at 80% of the whole container; fermenting under the conditions of light-proof and room temperature, opening the container every day to deflate during fermentation, stirring the materials floating on the liquid surface into the liquid, repeating the operation, and standing for 3 months under the condition of light-proof without deflating after one month.

The invention also discloses a planting method for improving the content of soluble sugar in the winter purple sweet potato by applying the enzyme, wherein the planting method comprises the following steps: diluting 10ml of enzyme stock solution by 2000 times at the 80 th day after the purple sweet potato seedlings are planted and spraying the purple sweet potato seedlings; then spraying 1 time every 7d, and spraying 3 times in total.

The invention has the following beneficial effects:

according to the planting method, early-stage spraying experiments are carried out on the enzyme of different raw materials, so that the soluble sugar content of the purple sweet potatoes of winter species can be remarkably improved, the kitchen waste is optimized and the raw material formula is optimized to obtain the enzyme raw material formula proportion and the preparation method suitable for the growth of the purple sweet potatoes, the kitchen waste is simply treated to obtain the efficient enzyme, the enzyme is sprayed into the purple sweet potatoes, the purpose of improving the soluble sugar content of the purple sweet potatoes Guijing potato No. 8 is achieved under the specific application method and conditions, the overall method is simple and easy to implement, meanwhile, the kitchen waste is effectively utilized, and the utilization capacity of the kitchen waste is improved.

[ detailed description ] embodiments

All of the features disclosed in this specification, or all of the steps in any method or process so disclosed, may be combined in any combination, except combinations of features and/or steps that are mutually exclusive.

Any feature disclosed in this specification (including any accompanying claims, abstract) is merely an example of a generic series of equivalent or similar features, unless explicitly described as such.

Example 1:

this example is a method for preparing ferment.

This example is a preparation method of enzyme used in the present invention, which includes the following steps:

1. selecting enzyme raw materials:

kitchen waste: rice (leftovers), vegetable waste (old vegetable leaves and vegetable stalks are mixed according to a mass ratio of 1:1, preferably the white vegetable leaves and the vegetable stalks), apple waste (apple peel and apple pit), pear waste (pear peel and pear pit), cucumber waste (cucumber peel), watermelon waste (watermelon peel), passion fruit (whole fruit) and lemon (whole fruit); the raw materials are not added with water during juicing or pulverizing.

The preparation method comprises the following steps: mixing and chopping kitchen waste (except passion fruit and/or lemon), crushing by using a stirrer to obtain kitchen waste slurry, mixing the kitchen waste slurry, sugar and water according to the mass ratio of 4:1:10, putting into a plastic barrel, uniformly mixing, adding sour fruit juice (namely passion fruit and/or lemon juice single product or mixture) with the mass of 5-10% of the total mixture, uniformly mixing, and keeping the charging amount of 80% of the whole container; fermenting in dark at room temperature, opening the container every day to release gas during fermentation, stirring the material floating on the liquid surface into the liquid, repeating the operation, no gas release after one month, standing in dark for 3 months, and filtering to obtain the ferment stock solution.

In this example, several ferments from different sources are provided, as shown in table 1:

TABLE 1 enzyme combination using different kitchen wastes as raw materials

The corresponding ferments were prepared according to the above groups, with reference to the preparation of example 1.

Example 2:

the application of the ferment in improving the soluble sugar of the sweet potato:

the planting time of the experimental group and the control (CK group) was: 11 months in 2018; the planting place is as follows: kyoho, North sea, Guangxi.

Experimental groups (4 replicates were designed per experimental group):

selecting 10ml of enzyme stock solution of groups 1-6 to dilute 1000 times, and spraying sweet potatoes, wherein the specific variety and spraying method are as follows:

II, variety: guang potato 87 (non-purple sweet potato), Guijing potato No. 8 (purple sweet potato);

thirdly, spraying: spraying the sweet potato leaves in the greenhouse at 20d, 40d, 60d and 80d after the transplanting and field planting of the sweet potato seedlings, wherein the uniform spraying of the sweet potato leaves is ensured when the spraying is carried out, and the spraying time is 18:00 in the afternoon every day. After the growth period is finished: sampling at 150d to measure the soluble sugar content of sweet potatoes in each group;

and (3) CK group: spraying no enzyme, performing the same water and fertilizer management as the experimental group, and culturing till 150d, sampling and measuring the soluble sugar content of each group of potato blocks; specifically, as shown in Table 2:

TABLE 2 influence of enzymes on soluble sugar content of sweet potato pieces

It can be known from table 2 that, in the above experiments, the enzymes of group 1 and group 4 have significant improvement effect on the soluble sugar content of the sweet potato tuber, while the enzymes of other groups have no significant improvement effect on the sweet potato tuber, and some enzymes are not beneficial to the improvement of the soluble sugar content of the sweet potato tuber, wherein the enzyme of group 4 has the most significant improvement effect on the soluble sugar content of sweet potato variety Guijing potato No. 8.

Example 3:

this example is based on the preferred enzyme group (group 4) of example 2, and the optimal application method of the enzyme for increasing the soluble sugar content is studied as follows:

the planting time of this embodiment is: 11 months in 2019; the planting place is as follows: kyoho, North sea, Guangxi.

Optimizing the formula of the enzyme raw material: in this embodiment, the orthogonal experiment is adopted to mix rice, apple waste, pear waste, watermelon waste and cucumber waste according to the same mass ratio and optimize the formula of passion fruit juice (the amount and steps of the passion fruit juice added are consistent with those in embodiment 1), the spraying method in embodiment 2 is adopted to spray purple sweet potatoes, and the soluble sugar content of the 150 th purple sweet potato tuber after spraying is specifically detected as shown in tables 3 to 4:

TABLE 3 influence of kitchen wastes on soluble sugar content of purple sweet potato tuberous root on orthogonal experimental factor level

TABLE 4 results of orthogonal experiments

Wherein the content of soluble sugar in the control CK group sweet potato tuber is 84.25 mg/g;

as can be seen from Table 4, the enzymes prepared by using the raw materials in different proportions have different influences on the soluble sugar content of the sweet potato tuber, and from high to low, the factors A, B, C, D, E and F are sequentially from high to low; it can be seen that the most significant factors in the above formulation are the adjusted addition of rice, and then the addition of apple waste, pear waste, watermelon waste, and cucumber waste, which affect the minimum addition of passion fruit juice. From this, it is inferred that, in the raw material for producing the enzyme capable of increasing the soluble sugar content of the purple sweet potato, the influence of the raw material having a higher sugar content on the experimental results is larger, and it can be seen from the above experiment that: compared with the soluble sugar content (84.25mg/g) of the CK control group, the soluble sugar content of some test groups sprayed with enzyme is reduced (test group 8), but the soluble sugar content of most test groups is higher than that of the CK control group; therefore, the enzyme produced by the same raw materials in different proportions has different influences on the content of soluble sugar in the sweet potatoes, formula optimization is needed, and the optimal test group for enzyme production is a test 6, namely A2B1C2D3E4F5, after orthogonal experiment optimization; namely, the corresponding preparation method comprises the following steps:

mixing and chopping kitchen waste (rice: apple waste: pear waste: watermelon waste: cucumber waste in a mass ratio of 2:1:2:3:4), crushing by using a stirrer to obtain kitchen waste slurry, mixing the kitchen waste slurry, sugar and water in a mass ratio of 4:1:10, putting into a plastic barrel, uniformly mixing, adding passion fruit juice accounting for 10% of the total mixture by mass, and uniformly mixing, wherein the charging amount is 80% of the whole container; fermenting under the conditions of light-proof and room temperature, opening the container every day to deflate during fermentation, stirring the materials floating on the liquid surface into the liquid, repeating the operation, and standing for 3 months under the condition of light-proof without deflating after one month.

Example 4:

in this example, the purple sweet potato is planted by the ferment prepared according to the optimal proportion in example 3.

The planting time of this embodiment is: year 2020, month 11; the planting place is as follows: kyoho, North sea, Guangxi.

The applicant finds that the effect of the enzyme on the content of soluble sugar in sweet potatoes is mainly in the following aspects in a large number of previous daily planting experiments: the spraying time; the number of spraying times; ③ the amount of spraying. Therefore, after continuous optimization of experiments, the applicant emphasizes the following treatment on the purple sweet potatoes, specifically as follows:

the spraying opportunity experiment:

the purple sweet potatoes are sprayed at different times in the growth period, 10ml of enzyme stock solution is diluted by 1000 times when the purple sweet potatoes are sprayed, and the spraying time for the first time is respectively (4 times are designed for each experimental group):

experimental group 1: spraying enzyme at the 40 th day after the purple sweet potato seedlings are transplanted and fixed; then spraying for 1 time every 7 days, and spraying for 4 times totally;

experimental group 2: spraying enzyme at the 60 th day after the purple sweet potato seedlings are transplanted and fixed; then spraying for 1 time every 7 days, and spraying for 4 times totally;

experimental group 3: spraying enzyme at 80d after the purple sweet potato seedlings are transplanted and fixed; then spraying for 1 time every 7 days, and spraying for 4 times totally;

experimental group 4: spraying enzyme at the 100 th day after the purple sweet potato seedlings are transplanted and fixed; then spraying for 1 time every 7 days, and spraying for 4 times totally;

CK: the method for planting and managing water and fertilizer is the same as the experimental groups 1-4 without spraying enzyme.

The soluble sugar content of the tuberous root of the purple sweet potato is measured by 150d of the full-breeding period, and is specifically shown in table 5:

TABLE 5 influence of different ferment spraying time on soluble sugar content of sweet potato root tuber

Test item	Experimental group 1	Experimental group 2	Experimental group 3	Experimental group 4	CK
						Soluble sugar (mg/g)	85.97	89.41	119.25	98.06	84.97

As can be seen from Table 5, the influence of different spraying occasions on the soluble sugar content of the sweet potato tuber is different, the enzyme is sprayed in the planting period, the effects of decomposing the fertilizer and promoting the absorption of crops are mainly achieved, the plants do not completely grow at an excessively fast application occasion, the nutrient substances in the fertilizer cannot be effectively absorbed, the application time is too slow, the plants cannot be effectively transformed and absorbed and then are harvested, and the soluble sugar content of the plants cannot reach the standard. Therefore, experiments prove that under the condition of an experimental group 3, namely, the enzyme is sprayed at the 80 th day after the purple sweet potato seedlings are transplanted and fixed; the enrichment of the soluble sugar of the purple sweet potatoes is the best experimental condition of the spraying time.

Secondly, spraying frequency experiment:

the method comprises the following steps of studying enzyme spraying on the purple sweet potatoes at different time of a growth period, diluting 10ml of enzyme stock solution by 1000 times when spraying, and selecting the optimal first spraying time obtained by the first group of experiments, namely spraying the enzyme at 80d after transplanting and field planting of the purple sweet potato seedlings, wherein the spraying frequency is 1-5 times, and the method is specifically as follows (4 times are designed for each experiment group):

experimental group a: spraying enzyme at 80d after the purple sweet potato seedlings are transplanted and fixed; spraying for 1 time only;

experimental group B: spraying enzyme at 80d after the purple sweet potato seedlings are transplanted and fixed; spraying for 1 time at intervals of 7d, and spraying for 2 times in total;

experimental group C: spraying enzyme at 80d after the purple sweet potato seedlings are transplanted and fixed; then spraying 1 time every 7 days, and spraying 3 times in total;

experimental group D: spraying enzyme at 80d after the purple sweet potato seedlings are transplanted and fixed; then spraying for 1 time every 7 days, and spraying for 4 times totally;

experimental group E: spraying enzyme at 80d after the purple sweet potato seedlings are transplanted and fixed; then spraying 1 time every 7 days, and spraying 5 times totally;

CK: no enzyme is sprayed, and other planting and water and fertilizer management methods are the same as those of the experimental groups A-E.

The soluble sugar content of the tuberous root of the purple sweet potato is measured by 150d of the full-breeding period, and is specifically shown in table 5:

TABLE 6 influence of different enzyme spraying times on soluble sugar content of sweet potato root tuber

Test item	Experimental group A	Experimental group B	Experimental group C	Experimental group D	Experimental group E	CK
							Soluble sugar (mg/g)	84.38	86.57	121.35	119.25	91.36	84.07

As can be seen from Table 6, the influence of different spraying times on the soluble sugar content of the sweet potato tuber is different, the spraying times are too few, and the enzyme is easily decomposed by environmental conditions, so that the enzyme can not be used for effectively decomposing the fertilizer macromolecules because the enzyme is not decomposed in time and is inactivated, and the plant can not be promoted to absorb and convert the nutrient components; the too many times of spraying can also cause that enzymes in the enzymes can influence the growth of plants, so that the effects of absorbing and converting nutrient components and enriching can not be achieved, and the enrichment of soluble sugar can not be achieved, therefore, experiments prove that the enzymes are sprayed at the 80 th day after the transplanting and field planting of the purple sweet potato seedlings under the condition of an experimental group C; then spraying 1 time every 7 days, and spraying 3 times in total; the enrichment of the soluble sugar of the purple sweet potatoes is the experimental condition of the optimal spraying times.

Thirdly, spraying amount experiment:

the purple sweet potatoes are sprayed at different time in the growth period, the best first spraying time obtained by the first group of experiments is selected, namely, enzyme is sprayed at the 80 th day after the purple sweet potato seedlings are transplanted and fixed, the best first spraying times obtained by the second group of experiments are selected, namely, the enzyme is sprayed for 3 times, and different spraying amounts are researched, specifically, the following steps are carried out (4 times are designed for each experiment group):

experimental group a: spraying enzyme on the leaves of the purple sweet potato plants by taking 10ml of enzyme stock solution to dilute 500 times of the solution at the 80 th day after the transplantation and the field planting of the purple sweet potato seedlings; completely wetting the leaves, and then spraying for 1 time at intervals of 7d for 3 times; the spraying amount is the same each time;

experimental group b: spraying enzyme on the leaves of the purple sweet potato plants by taking 10ml of enzyme stock solution to dilute 1000 times of the solution at the 80 th day after the transplantation and the field planting of the purple sweet potato seedlings; completely wetting the leaves, and then spraying for 1 time at intervals of 7d for 3 times; the spraying amount is the same each time;

experimental group c: spraying enzyme on the leaves of the purple sweet potato plants by taking 10ml of enzyme stock solution to dilute 2000 times of the solution at the 80 th day after the transplantation and the permanent planting of the purple sweet potato seedlings; completely wetting the leaves, and then spraying for 1 time at intervals of 7d for 3 times; the spraying amount is the same each time;

experimental group d: spraying enzyme on the leaves of the purple sweet potato plants by taking 10ml of enzyme stock solution to dilute 3000 times of the solution at the 80 th day after the transplanting and field planting of the purple sweet potato seedlings; completely wetting the leaves, and then spraying for 1 time at intervals of 7d for 3 times; the spraying amount is the same each time;

CK: no enzyme is sprayed, and other planting and water and fertilizer management methods are the same as those of the experimental groups A-E.

The soluble sugar content of the tuberous root of the purple sweet potato is measured by 150d of the full-breeding period, and is specifically shown in table 5:

TABLE 7 influence of different enzyme spraying amounts on the soluble sugar content of sweet potato root tuber

Test item	Experimental group a	Experimental group b	Experimental group c	Experimental group d	CK
						Soluble sugar (mg/g)	93.58	121.35	135.25	85.47	84.07

As can be seen from Table 7, the influence of different spraying amounts on the soluble sugar content of the sweet potato tuber is different, the spraying amount is too small, and the enzyme is easily decomposed by environmental conditions, so that the enzyme can be inactivated without having to decompose the fertilizer, cannot effectively play a role in decomposing the macromolecules of the fertilizer, and cannot promote the absorption and the transformation of the nutrient components of the plants; the excessive spraying amount also can cause that enzyme in the enzyme can influence the growth of plants, so that the effects of absorbing and converting nutrient components and enriching can not be achieved, and the enrichment of soluble sugar can not be achieved, therefore, experiments prove that the enzyme is sprayed at the 80 th day after the transplanting and field planting of the purple sweet potato seedlings under the condition of an experimental group b; diluting 10ml of ferment stock solution by 2000 times when spraying; then spraying 1 time every 7 days, and spraying 3 times in total; the condition is the best spraying condition for enriching the soluble sugar of the purple sweet potato of the winter variety.

In conclusion, the enzyme has a good promoting effect on the content of soluble sugar in the purple sweet potatoes, but the requirement on spraying conditions is strict, and the optimal spraying conditions are that 10ml of enzyme stock solution is diluted by 2000 times to be sprayed on the purple sweet potato seedlings at 80d after the purple sweet potato seedlings are planted; then spraying 1 time every 7d, and spraying 3 times in total.

Wherein, under the spraying condition, the preparation method of the ferment can achieve the best effect: mixing and chopping kitchen waste (rice: apple waste: pear waste: watermelon waste: cucumber waste in a mass ratio of 2:1:2:3:4), crushing by using a stirrer to obtain kitchen waste slurry, mixing the kitchen waste slurry, sugar and water in a mass ratio of 4:1:10, putting into a plastic barrel, uniformly mixing, adding passion fruit juice accounting for 10% of the total mixture by mass, and uniformly mixing, wherein the charging amount is 80% of the whole container; fermenting under the conditions of light-proof and room temperature, opening the container every day to deflate during fermentation, stirring the materials floating on the liquid surface into the liquid, repeating the operation, and standing for 3 months under the condition of light-proof without deflating after one month.

To sum up, the enzyme that this application was obtained to the raw materials preparation is studied, obtains how recycle kitchen garbage preparation enzyme's method to carry out reasonable ratio optimization to the preparation raw materials, obtain the enzyme that can effectively improve purple sweet potato root piece soluble sugar content, obtain the best application condition of this enzyme through improvement spraying method, under this specific condition, the soluble sugar content of this application purple sweet potato root piece obtains effectively improving.

The above examples are merely illustrative of several embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.