阅读说明：本技术 一种同时获得全氟化碳纳米探针19f-mr弛豫时间及其加权图像的成像方法 (It is a kind of to obtain perfluorocarbon probe simultaneously19The imaging method of F-MR relaxation time and its weighted image ) 是由 孙夕林 吴丽娜 刘爽 卫佳楠 王凯 杨丽丽 阿荣 李晓娜 李迎波 杨洁 程永娜 于 2019-08-07 设计创作，主要内容包括：本发明涉及医学成像领域,即一种用于同时获得所测对象的~(19)F核自旋系统的T1纵向弛豫时间及其T1加权信号的~(19)F磁共振成像方法,其步骤如下：一、使用一种含氟造影剂全氟化碳纳米粒子(Perfluorocarbon Nanoparticles,PFC NPs)利用~(19)F_T1mapRAREVTR序列,调整多个不同重复时间进行成像；二、用~1H/~(19)F双核表面线圈采集全氟化碳纳米粒子的磁共振信号,产生多个扫描图像,用于不同重复时间条件下T1弛豫值的计算；三、基于多个扫描图像,通过所述T1弛豫时间计算所述多个不同重复时间其中之一处的T1加权图像,计算扫描对象的T1信号。研究表明,本发明能够同时得到扫描对象核自旋系统的纵向弛豫时间和T1加权图像,能更快更简便的得到成像信息,简化操作流程和步骤,提高所测对象的~(19)F核自旋系统的检测效率。(It is the present invention relates to medical imaging field, i.e., a kind of for obtaining surveyed object simultaneously 19 The T1 longitudinal relaxation time of F nuclear spin system and its T1 weighted signal 19 F MR imaging method, its step are as follows: one, being utilized using a kind of fluorine-containing contrast agent perfluorocarbon particle (Perfluorocarbon Nanoparticles, PFC NPs) 19 F_T1mapRAREVTR sequence, adjusting multiple and different repetition times is imaged；Two, it uses 1 H/ 19 F double-core surface coils acquires the magnetic resonance signal of perfluorocarbon particle, generates multiple scan images, the calculating for T1 relaxation value under the conditions of the different repetition times；Three, multiple scan images are based on, the t1 weighted image at one of the multiple different repetition times place is calculated by the T1 relaxation time, calculates the T1 signal of sweep object.Studies have shown that the present invention can obtain the longitudinal relaxation time and t1 weighted image of sweep object nuclear spin system simultaneously, can be faster easier obtain image-forming information, streamline operation and step, improve surveyed object 19 The detection efficiency of F nuclear spin system.)

1. a kind of obtain perfluorocarbon probe simultaneously¹⁹The imaging method of F-MR relaxation time and its weighted image, feature It is that steps are as follows:

Nanometer¹⁹The preparation of F magnetic resonance molecular imaging probe:

(l) one or more of surfactants are uniformly mixed, is dissolved with the mixed solvent of chloroform and methanol, by dissolution Surfactant is evaporated by Rotary Evaporators, is dried overnight in 40 DEG C of vacuum drying ovens, last ultrasonic vibration is scattered in it In water, the blend of surfactant is obtained, it is spare；

(2) perfluocarbon, glycerol are dispersed in the blend for the table activating agent that step (l) obtains, in high pressure homogenizer Middle mixing, be made containing¹⁹The lotion of F nanoparticle；

(3) lotion that step (2) obtains gets rid of the component of cladding not yet in effect by the way of dialysis, obtains the nanometer¹⁹F Molecular imaging probe；

(4) probe solution that step (3) obtains is blended with 1.7% Ago-Gel, prepares and forms 200 μ L various concentrations PFOB nano molecular image probe, final concentration are 776,388,194,97,48.5mmol/L sample respectively；

Imaging method:

(a) object to be checked is disposed in the magnetic field center of 9.4T toy magnetic resonance, using binary channels coil¹H/¹⁹F line Circle carries out T1W RARE image scanning, obtains¹H Anatomical orientation image；It is right¹⁹F most intensive frequency spectrum group acquires signal, frequency values 376.64 MHZ are adjusted to, are carried out¹⁹F image scanning, obtain with¹H image common location¹⁹F image carries out conventional sequence later¹⁹F_ The scanning of T1mapRAREVTR imaging sequence, obtains traditional multiple and different repetition time TR's¹⁹F image；

(b) in order to obtain surveyed object simultaneously¹⁹The T1 longitudinal relaxation time and its t1 weighted image of F nuclear spin system, at As parameter is further improved；By analyzing and comparing each method, selects and be most suitable for while obtaining¹⁹F T1 value and T1 weighted graph Picture and T1 signal；

The ms of ms, TR=400,800,1000,1500,2000,3000,4000,6000 of method 1:TE=156, NA=18；

The ms of ms, TR=400,800,1000,1500,2000,3000,4000,6000 of method 2:TE=156, NA=24；

The ms of ms, TR=400,800,1000,1500,2000,3000,4000,6000 of method 3:TE=156, NA=30；

The ms of ms, TR=400,800,1000,1500,2000,3000,4000,6000 of method 4:TE=156, NA=36；

(c) surveyed object is obtained based on step (b) content simultaneously¹⁹The T1 longitudinal relaxation time of F nuclear spin system and its The method of T1 weighted signal, while measuring various concentration sweep object¹⁹F signal-to-noise ratio and¹⁹F T1 value.

2. described in accordance with the claim 1 while obtaining perfluorocarbon probe¹⁹F-MR relaxation time and its weighted image Imaging method, it is characterised in that the perfluocarbon is bromination perfluorooctane.

3. described in accordance with the claim 1 while obtaining perfluorocarbon probe¹⁹F-MR relaxation time and its weighted image Imaging method, it is characterised in that in the imaging method, using following parameter: the ms of TE=18.56, Bandwidth=100 kHZ, TR=400,800,1000,1500,2000,3000,4000,6000 ms,NA=30,RARE Factor=8, Matrix= 64×64,FOV=38.4×38.4 mm2,Flip Angle=90°,Slice Thickness=3.0 mm。

4. described in accordance with the claim 1 while obtaining perfluorocarbon probe¹⁹F-MR relaxation time and its weighted image Imaging method, it is characterised in that the surfactant be dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, The mixture of dipalmitoylphosphatidylglycerol and cholesterol, the mol ratio between them are 75:2:5:18.

5. described in accordance with the claim 1 while obtaining perfluorocarbon probe¹⁹F-MR relaxation time and its weighted image Imaging method, it is characterised in that the mass ratio of the perfluocarbon and glycerol is 10-30:1.

Technical field

It is the present invention relates to medical imaging procedure field is belonged to, i.e., a kind of to obtain perfluorocarbon probe simultaneously¹⁹F-MR relaxation The imaging method of time and its weighted image.

Background technique

In the prior art, magnetic field is directed to individual nuclear spin according to the magnetic field strength applied and generates different energy levels, energy It is enough by application to there is the RF pulse-to-pulse for defining frequency (so-called Larmor frequency or MR frequency) to bring the individual nuclear spin of excitation (certainly Rotation resonance).Magnetic resonance imaging (Magnetic resonance imaging, MRI) is current routine clinical auxiliary examination methods, Have many advantages, such as that imaging parameters are more, scanning speed is fast, tissue high resolution and image definition are high, doctor's discovery can be helped to be not easy The early lesion discovered has become the important means of tumour, heart disease and cranial vascular disease early screening at present.

¹⁹F magnetic resonance imaging (¹⁹F-MRI) since it is with non-invasive, high without endogenous background interference and spatial resolution The advantages that, it is received more and more attention in the fields such as bio-imaging and medical diagnosis on disease.¹⁹The free abundance of F core is 100%, spin Quantum number is 1/2, with proton (¹H it) compares, gyromagnetic ratio is very high (94%, gyromagnetic ratio is maximum in multicore element), sensitivity 83%, Chemical shift is larger (400ppm),¹⁹F is a kind of magnetic resonance nucleic to serve many purposes.With traditional proton molecular imaging phase Than,¹⁹The advantage of F-MRI is that, due to lacking endogenous fluorine signal in biological tissue, the background signal of biological sample is almost nil, There is no background signal interference, meanwhile,¹⁹F-MRI does not need to pre-process, and does not need contrast agent, therefore¹⁹The multifrequency nature of F makes it Ideal object as research biological process.

Perfluocarbon (PFCs) is a kind of inert chemi-cal substance, is a kind of¹H proton quilt completely¹⁹The hydro carbons that F atom replaces Compound, safe and non-toxic and biological property are stablized, and have good biocompatibility, obtain already extensively in clinical medicine domain Using.Compared with other fluorochemicals, perfluocarbon is with higher¹⁹F atom content, sensibility are high, and perfluocarbon into After entering in vivo, it is not involved in internal metabolism, is removed eventually by phagocyte, therefore perfluocarbon will not be to intracorporal group It knits organ and generates toxicity.Perfluocarbon has become to be most commonly employed at present¹⁹The fluorochemical of F-MR imaging.

In clinical studies, the imaging of monitoring drug and curative effect are of great significance, and such as monitor the enrichment of inside tumor drug And intake, effect and metabolic condition etc. of the drug in tumour and histoorgan.It monitors drug imaging and curative effect requires to define Cause the concentration of the changed contrast agent of MR relaxation rate R1.Wherein, R1 is the inverse (1/T1) in T1 relaxation time, also referred to as SPIN-LATTICE RELAXATION process.Longitudinal relaxation time (T1) reflection tissue in proton energy be discharged into surrounding lattice or from The time parameter of the speed of surrounding lattice absorption energy, the size of T1 depend on the phase between external magnetic field and proton and ambient enviroment Interaction, this is the inherent characteristic of tissue, and after external magnetic field is given, the T1 value of different tissues is fixed.T1map technology is Measure a kind of MR quantitative imaging technique of voxel T1 value.According to the relationship between the signal strength of scanning sequence and voxel T1 value, The T1 value of each voxel in acquisition range is calculated according to formula.¹⁹F magnetic resonance T1 longitudinal relaxation time and signal measuring skill Art is the means for assessing surveyed object fluorine content, has become the main iconography detection method of current fluorine-containing material.In At present in the research of fluorine-containing material, usually it is divided to two sequence step measurements, first obtains surveyed object¹⁹F t1 weighted image, By weighted image collected, sweep object is calculated¹⁹F signal noise ratio (snr) of image recycles¹⁹F_T1mapRAREVTR sequence is come Measure surveyed substance¹⁹F T1 value.But current scanning sequence needs a point multiple steps to carry out image and data acquisition, Bu Nengtong When obtain¹⁹The T1 longitudinal relaxation time and its t1 weighted image of F nuclear spin system, operating process and step need further letter Change, to improve surveyed object¹⁹The detection efficiency of F nuclear spin system, optimization¹⁹F multicore imaging sequence and imaging method.

To sum up, perfluocarbon is to be most commonly employed at present¹⁹The fluorochemical of F-MR imaging, obtains surveyed object¹⁹F Development and life of the method for the T1 longitudinal relaxation time of nuclear spin system and its t1 weighted image in multiple nmr imaging technology Great potential is shown in object research.The present invention is based on existing¹⁹F multicore imaging sequence designs one kind and is used for while obtaining institute Survey object¹⁹The T1 longitudinal relaxation time of F nuclear spin system and its t1 weighted image signal¹⁹F MR imaging method, mentions Out by the signal of surveyed object and T1 value the synchronization gain in an imaging sequence imaging method.Image quality is not being influenced into as far as possible Sequential parameter is adjusted on the basis of amount, realizes surveyed object¹⁹The T1 longitudinal relaxation time and its T1 of F nuclear spin system weight letter Number synchronization gain, can be faster easier obtain image-forming information, streamline operation and step, improve surveyed object¹⁹F core is certainly The detection efficiency of rotation system.

Summary of the invention

Object of the present invention is to provide a kind of in view of the above deficiencies while obtaining perfluorocarbon probe¹⁹When F-MR relaxation Between and its weighted image imaging method, can be faster easier obtain image-forming information, streamline operation and step, improve institute Survey object¹⁹The detection efficiency of F nuclear spin system.

The technical scheme is that

It is a kind of to obtain perfluorocarbon probe simultaneously¹⁹The imaging method of F-MR relaxation time and its weighted image, feature exist In steps are as follows:

¹⁹The preparation of F magnetic resonance nano molecular imaging probe:

(l) one or more of surfactants are uniformly mixed, is dissolved with the mixed solvent of chloroform and methanol, by dissolution Surfactant is evaporated by Rotary Evaporators, is dried in 40 DEG C of vacuum drying ovens overnight, finally by the mode of ultrasonic vibration It breaks up and is dispersed in water, obtain the mixture of surfactant.

(2) perfluocarbon and glycerol are dispersed in the mixture for the surfactant that step (l) obtains, in high pressure In homogenizer be uniformly mixed, be made containing¹⁹The lotion of F nanoparticle.

(3) lotion that step (2) obtains gets rid of the component that do not wrapped up effectively by the way of dialysis, obtains described Contain¹⁹F nanoparticle¹⁹F magnetic resonance nano molecular imaging probe.

(4) the nano-probe lotion that step (3) obtains is blended with 1.7% agar sugar sol, it is dense is configured to 200 μ L differences The PFOB nano molecular image probe of degree (ultimate density is respectively 776,388,194,97,48.5mmol/L).

In above scheme, the surfactant is dipalmitoylphosphatidylcholine (DPPC), two palmityl phosphatidyls The mixture of ethanol amine (DPPE), dipalmitoylphosphatidylglycerol (DPPG) and cholesterol, the mol ratio between them are DPPC:DPPE:DPPG: cholesterol=75:2:5:18.

The perfluocarbon is bromination perfluorooctane (PFOB).

Imaging method:

(a) to be checked¹⁹F nano molecular image probe be disposed in the magnetic resonance of 9.4T toy (94/20 USR of BioSpec, Bruker, Germany) magnetic field center, using binary channels coil¹H/¹⁹F coil carries out T1W RARE(fast spin echo Sequence) image scanning, it obtains¹H Anatomical orientation image；It is right¹⁹F most intensive frequency spectrum group acquires signal, and frequency values are adjusted to 376.64MHZ is carried out¹⁹F image scanning, obtain with¹H image common location¹⁹F image carries out conventional sequence later¹⁹F_ The scanning of T1mapRAREVTR imaging sequence, obtains traditional multiple and different repetition time TR's¹⁹F image.

(b) in order to obtain surveyed object simultaneously¹⁹The T1 longitudinal relaxation time and its t1 weighted image of F nuclear spin system, Imaging parameters are further improved；By analyzing and comparing each method, selects and be most suitable for while obtaining¹⁹F T1 value and T1 add Weight graph picture and T1 signal.

The method 1:TE(echo time)=156 ms, the TR(repetition time)=400,800,1000,1500,2000,3000, 4000,6000 ms, NA(motivate number)=18.

The ms of ms, TR=400,800,1000,1500,2000,3000,4000,6000 of method 2:TE=156, NA=24.

The ms of ms, TR=400,800,1000,1500,2000,3000,4000,6000 of method 3:TE=156, NA=30.

The ms of ms, TR=400,800,1000,1500,2000,3000,4000,6000 of method 4:TE=156, NA=36.

(c) surveyed object is obtained based on content described in step b simultaneously¹⁹The T1 longitudinal relaxation time of F nuclear spin system and The method of its T1 weighted signal, while measuring various concentration sweep object¹⁹F signal-to-noise ratio (Signal-to-Noise Ratio, SNR) value and¹⁹F T1 value.

In the imaging method, using following parameter: the kHZ of ms, Bandwidth=100 of TE=18.56, TR=400, 800,1000,1500,2000,3000,4000,6000 ms,NA=30,RARE Factor=8, Matrix=64×64,FOV= 38.4×38.4 mm2,Flip Angle=90°,Slice Thickness=3.0 mm。

Pseudo- coloured silk is added in last handling process, delimited area-of-interest (Region of Interest, ROI), and measurement is not With concentration sweep object¹⁹F T1 value and¹⁹F signal-to-noise ratio.In order to obtain¹⁹F signal-to-noise ratio data, uses ParaVision Acquisition 6.0.1 system is by calculating the t1 weighted image analysis at one of multiple and different repetition times place¹⁹F letter It makes an uproar than (to control variable, selection and tradition¹⁹Scan image under F sequence identical parameters when TR=4000 ms carries out analysis pair Than).In order to obtain¹⁹F T1 data are based on passing through scan image production using ParaVision Acquisition 6.0.1 system Raw multiple scan images, pass through Single-Index Model M_z(t)=M₀(1-e-^t/T1) matched curve, quantitative analysis T1 value.

The present invention is utilized using a kind of fluorine-containing contrast agent PFOB¹⁹F_T1map sequence adjusts multiple and different repetition times progress Imaging.With¹H/¹⁹F double-core surface coils acquires the magnetic resonance signal of perfluorocarbon particle, generates multiple scan images, uses The calculating of T1 relaxation value under the conditions of the different repetition times.Based on multiple scan images, institute is calculated by the T1 relaxation time The t1 weighted image for stating one of multiple and different repetition times place calculates the T1 signal of sweep object.

Studies have shown that be imaged using perfluorocarbon molecular imaging probe PFOB of the present invention to synthesis, it can Its T1 value of easier acquisition and its T1 weighted signal improve surveyed object¹⁹The detection efficiency of F nuclear spin system.

The invention has the advantages that this method is in perfluorocarbon molecular imaging probe in detecting, it was demonstrated that this method It is detecting¹⁹There is unique advantage, when can obtain the longitudinal relaxation of sweep object nuclear spin system simultaneously on F nuclear spin system Between and t1 weighted image, can be faster easier obtain image-forming information, streamline operation and step, improve surveyed object¹⁹F The detection efficiency of nuclear spin system, to realize that the imaging method is applied to vivo tumor imaging and diagnosis and treatment integration provides technology branch It holds, can be improved tumor cells and be imaged on body detection efficiency, imaging in vivo and diagnosis are carried out to tumour from molecular biology level And the assessment for the treatment of curative effect.

Embodiments of the present invention are described in further detail below in conjunction with embodiment.

Detailed description of the invention

Fig. 1 is PFOB nano molecular image probe¹⁹F-MRI various concentration sample¹H and¹⁹F-MRI image.

Fig. 2 is PFOB nano molecular image probe under conventional imaging method and method¹⁹F R1 and concentration relationship figure, noise Than (SNR) and concentration relationship figure and method 3¹⁹F image.

Fig. 3 be conventional imaging method (as figure a) with method (as figure b-e) under, PFOB nano molecular image probe¹⁹F- MRI various concentration sample R1 and signal to noise ratio (snr) relational graph.

Specific embodiment

It is a kind of while obtaining surveyed object referring to Fig. 1,2,3¹⁹The T1 longitudinal relaxation time and its T1 of F nuclear spin system Weighted image¹⁹F MR imaging method, described method includes following steps:

1. nanometer¹⁹The preparation of F magnetic resonance molecular imaging probe

(l) dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylethanolamine (DPPE), two palmityl phosphatidyls are sweet Oily (DPPG) and cholesterol uniformly mix, and mol ratio is DPPC:DPPE:DPPG: cholesterol=75:2:5:18, molten with chloroform Solution, is evaporated by Rotary Evaporators, is dried overnight in 40 DEG C of vacuum drying ovens, last ultrasonic vibration is dispersed in water it, obtains It is spare to the blend of surfactant.

(2) perfluocarbon, glycerol are dispersed in the blend for the surfactant that step (l) obtains, it is perfluorinated The mass ratio of carbon and glycerol is 10-30:1, mixes in high pressure homogenizer, is made and contains¹⁹The lotion of F nanoparticle.Wherein perfluor Change carbon, glycerol account for the 20% of gross mass altogether, and water accounts for the 77% of gross mass, and surfactant accounts for the 3% of gross mass.

(3) surfactant of step (2) obtains lotion dialysis cladding not yet in effect to remove, obtains described receive Rice¹⁹F magnetic resonance molecular imaging probe.

(4) the PFOB nano molecular image probe solution that step (3) obtains is blended with 1.7% agar sugar sol, prepares shape At the PFOB nano molecular image probe of 200 μ L various concentrations, final concentration is 776,388,194,97,48.5 mmol/L respectively Sample.

2. imaging method is applied to nanometer¹⁹F magnetic resonance molecular imaging probe obtains simultaneously¹⁹The T1 of F nuclear spin system is longitudinal Relaxation time and its t1 weighted image

(l) object to be checked be disposed in the magnetic resonance of 9.4T toy (BioSpec 94/20 USR, Bruker, Germany magnetic field center).Using binary channels coil¹H/¹⁹F coil carries out T1W RARE(fast acquisition interleaved spin echo) imaging Scanning obtains¹H Anatomical orientation image.Sweep parameter is TR=800ms, TE=12 ms, NA=4, Matrix(matrix)=256 × 256, RARE Factor(RARE accelerated factors)=8, FOV(scan vision)=38.4 × 38.4 mm², Flip Angle(overturning Angle)=90 °, Slice Thickness(thickness)=1.0 mm.As a result as shown in Fig. 2, can be seen that synthesis from the result The 1H signal enhancing ability of perfluorocarbon molecular imaging probe and concentration of specimens are there are closely related, as concentration of specimens mentions Height, signal enhancing.

(2) binary channels coil is used¹H/¹⁹F coil carries out¹⁹F image scanning, obtain with¹H image common location¹⁹F image. Multicore channel is adjusted to the channel F, in view of synthesis PFOB nano-probe belong to it is more¹⁹The compound of F frequency spectrum, needs pair¹⁹F is most close The frequency spectrum group of collection acquires signal, passes through SINGLEPULSE_¹⁹F sequence, to most of fluorine core of PFOB (in 17 fluorine atom cores 12) CF₃Peak resonant frequency, frequency values are adjusted to 376.64 MHZ, and to CF₃Peak carries out shimming.

TR/TE=4000/156 ms, Bandwidth(bandwidth)=50 kHZ, NA=128, Factor=64 RARE, Matrix=64×64,FOV=38.4×38.4 mm²,Flip Angle=90°,Slice Thickness=3.0 mm.As a result such as Shown in Fig. 2, the perfluorocarbon molecular imaging probe of synthesis can be seen that from the result¹⁹F signal enhancing ability and sample This concentration is positively correlated, as concentration of specimens improves, linearly enhancing.

(3) binary channels coil is used¹H/¹⁹F coil carries out conventional sequence¹⁹The scanning of F_T1mapRAREVTR imaging sequence, is obtained Obtain traditional multiple and different repetition time TR's¹⁹F image.TE=18.56ms,Bandwidth=100 kHZ,TR=400, 800,1000,1500,2000,3000,4000,6000ms,NA=6,RAREFactor=8,Matrix=64×64,FOV=38.4 ×38.4 mm²,Flip Angle=90°,Slice Thickness=3.0 mm.It as a result as shown in Fig. 2, can be with from the result Find out, the perfluorocarbon molecular imaging probe of synthesis¹⁹F T1 value is positively correlated with concentration of specimens, as concentration of specimens mentions Height, T1 value increase, related coefficient 0.9906.

(4) in order to obtain surveyed object simultaneously¹⁹The T1 longitudinal relaxation time and its t1 weighted image of F nuclear spin system, Imaging parameters are further improved.By analyzing and comparing each method, selects and be most suitable for while obtaining¹⁹F T1 value and T1 add Weight graph picture and T1 signal.

The ms of ms, TR=400,800,1000,1500,2000,3000,4000,6000 of method 1:TE=156, NA=18.

Method 2:TE=156 ms, TR=400,800,1000,1500,2000,3000,4000,6000ms, NA=24.

Method 3:TE=156 ms, TR=400,800,1000,1500,2000,3000,4000,6000ms, NA=30.

Method 4:TE=156 ms, TR=400,800,1000,1500,2000,3000,4000,6000ms, NA=36.

3. adding pseudo- coloured silk in last handling process, region of interest domain measurement various concentration sweep object delimited¹⁹F T1 value With¹⁹F SNR value.In order to obtain¹⁹F signal-to-noise ratio data passes through calculating using ParaVision Acquisition 6.0.1 system T1 weighted image at one of multiple and different repetition times place is analyzed¹⁹F signal-to-noise ratio (selection and tradition¹⁹The identical ginseng of F sequence Scan image when several lower TR=4000 ms carries out analysis comparison).In order to obtain¹⁹F T1 data, use ParaVision Acquisition 6.0.1 system passes through Single-Index Model M based on the multiple scan images generated by scan image_z(t)= M₀(1-e-^t/T1) matched curve, quantitative analysis T1 value.As a result as shown in Fig. 2, from the result as can be seen that in each method, Measured various concentration perfluorocarbon molecular imaging probe¹⁹F signal is positively correlated with concentration of specimens, as sample is dense Degree improves, linearly enhancing, wherein in each method, by compared with traditional sequence measuring (such as Fig. 2), and gained image noise Preferably two methods are method 2, method 3 than in.Meanwhile in each method, measured various concentration perfluorocarbon Molecular imaging probe¹⁹F T1 value is positively correlated with concentration of specimens, as concentration of specimens improves, T1 value increase, by with tradition Sequence measuring (such as Fig. 2) compare, related coefficient measured by each method is respectively R²=0.9906, R²=0.7762, R²= 0.8029, R²=0.9764, R²=0.8374, preferably two methods are method 3, method 4.Further analysis¹⁹F T1 value with¹⁹F The relationship of signal-to-noise ratio, the present invention analyze and compare signal-to-noise ratio with¹⁹The linearly dependent coefficient (such as Fig. 3) of F T1, traditional measurement side Method related coefficient is 0.9521, compares each method, and method 3 and method 4 are excellent, and measurement related coefficient is respectively 0.9615,0.992. Finally, compared with comprehensive analysis is measured with conventional method¹⁹F T1 value with¹⁹F signal-to-noise ratio, the present invention select to use method 3 as and meanwhile obtain Obtain surveyed object¹⁹The T1 longitudinal relaxation time of F nuclear spin system and its t1 weighted image¹⁹F MR imaging method, tool Swept-volume parameter is as follows: the kHZ of TE=18.56ms, Bandwidth=100, TR=400,800,1000,1500,2000,3000, 4000,6000ms,NA=30,RARE Factor=8, Matrix=64×64,FOV=38.4×38.4 mm²,Flip Angle= 90°,Slice Thickness=3.0 mm。