阅读说明：本技术 一种含铁皮石斛提取物的洁面乳及其制备方法 (Facial cleanser containing dendrobium officinale extract and preparation method thereof ) 是由 刘裕娇 庞丽婷 招敏聪 贺锐 杨悬 于 2021-09-06 设计创作，主要内容包括：本发明提供了一种含铁皮石斛提取物的洁面乳及其制备方法,属于化妆品技术领域,该洁面乳包括植物提取物A、植物提取物B、琥珀酸酯类表面活性剂、氨基酸类表面活性剂、两性离子表面活性剂、保湿剂、乳化剂、鲸蜡硬脂醇、氯化钠、增稠剂、肤感调节剂、对羟基苯乙酮和1,2-己二醇；其中植物提取物A包括铁皮石斛提取物和明日叶提取物；植物提取物B包括金钗石斛提取物、马鞭草提取物、野玫瑰提取物和雪莲花提取物。该洁面乳制备方法简单、高效,温和无刺激,去污效果较佳。(The invention provides a facial cleanser containing dendrobium officinale extract and a preparation method thereof, belonging to the technical field of cosmetics, wherein the facial cleanser comprises a plant extract A, a plant extract B, a succinate surfactant, an amino acid surfactant, a zwitterionic surfactant, a humectant, an emulsifier, cetearyl alcohol, sodium chloride, a thickening agent, a skin feel modifier, p-hydroxyacetophenone and 1, 2-hexanediol; wherein the plant extract A comprises herba Dendrobii extract and Angelica keiskei extract; the plant extract B comprises herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Saussureae Involueratae extract. The facial cleanser has the advantages of simple preparation method, high efficiency, mildness, no irritation, and good decontamination effect.)

1. The facial cleanser containing the dendrobium officinale extract is characterized in that: comprises a plant extract A, a plant extract B, a succinate surfactant, an amino acid surfactant, a zwitterionic surfactant, a humectant, an emulsifier, cetostearyl alcohol, sodium chloride, a thickening agent, a skin feel regulator, p-hydroxyacetophenone and 1, 2-hexanediol;

the plant extract A comprises dendrobium officinale extract and angelica keiskei extract;

the plant extract B comprises herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Adonidis extract.

2. The facial cleanser according to claim 1, characterized in that: the facial cleanser comprises the following components in parts by weight: 0.5-3 parts of plant extract A, 0.3-1.2 parts of plant extract B, 30-45 parts of succinate surfactant, 10-25 parts of amino acid surfactant, 6-22 parts of zwitterionic surfactant, 8-20 parts of humectant, 0.1-0.5 part of emulsifier, 1-5 parts of cetearyl alcohol, 0.5-4 parts of sodium chloride, 0.5-5 parts of thickening agent, 0.5-5.5 parts of skin feel regulator, 0.6-0.7 part of p-hydroxyacetophenone and 0.3-1.1 part of 1, 2-hexanediol.

3. The facial cleanser according to claim 1, characterized in that: the plant extract A is a dendrobium officinale extract and a angelica keiskei extract in a weight ratio of 3-9: 2.

4. The facial cleanser according to claim 1, characterized in that: the plant extract B is a dendrobium nobile extract, a verbena extract, a wild rose extract and a snow lotus herb extract in a weight ratio of 3-5:1-2:1-2: 1.

5. The facial cleanser according to claim 1, characterized in that: the succinate surfactant is disodium lauryl sulfosuccinate.

6. The facial cleanser according to claim 1, characterized in that: the amino acid surfactant is one or more of sodium lauroyl sarcosinate, TEA (TEA cocoyl glutamate) salt and sodium lauroyl glutamate.

7. The facial cleanser according to claim 1, characterized in that: the zwitterionic surfactant is cocamidopropyl betaine.

8. The facial cleanser according to claim 1, characterized in that: the humectant is one or more of glycerol, diglycerol and polyethylene glycol-8; the emulsifier is methyl glucose sesquistearate; the thickening agent is acrylic acid (ester) copolymer; the skin feeling regulator is polyquaternium-7.

9. The facial cleanser according to claim 1, characterized in that: the weight ratio of the plant extract A to the plant extract B is 2-3: 1.

10. The method for preparing a facial cleanser according to any one of claims 1 to 9, comprising: the method comprises the following steps:

(1) adding succinate surfactant, amino acid surfactant, zwitterionic surfactant, humectant, emulsifier and stearin into an emulsifying pot, heating and stirring, and keeping the temperature until the components are completely dissolved to obtain transparent liquid;

(2) mixing sodium chloride and water, adding the mixture into the transparent liquid obtained in the step (1) to obtain a mixture A, and cooling until the mixture A is solidified and uniform;

(3) adding p-hydroxyacetophenone and 1, 2-hexanediol into the mixture A at 60-70 ℃, cooling to 55-60 ℃, slowly adding the thickening agent, the water and the mixture while stirring, adding the mixture of the skin feel modifier and the water after uniformly stirring, and adding the plant extract A after uniformly stirring to obtain a mixture B;

(4) stirring plant extract B and ascorbyl palmitate at 50-55 deg.C, cooling to 40-45 deg.C, filtering, and discharging to obtain facial cleanser.

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a facial cleanser containing dendrobium officinale extract and a preparation method thereof.

Background

The face cleansing cream is generally used for cleaning dirt, dust, microorganisms, body surface grease, aged cutin, sweat stain and residues of cosmetics on the surface of the skin, thereby ensuring the refreshing and moisturizing normal physiological functions of the skin. The traditional face cleansing cream focuses on high-efficiency cleaning and oil removal, so that components such as a surfactant with strong alkalinity or a soap base and the like are usually added, and the skin is irritated and pores are enlarged after the traditional face cleansing cream is used for a long time. Over time, natural, non-irritating and green facial cleanser gradually replaces the traditional facial cleanser to become the most popular choice, and the facial cleanser containing plant extracts is more favored.

Among various plant extracts, the dendrobium extract has been extensively studied, such as xuhong, liu drastic, wang toweling, etc. 5 kinds of dendrobium and tissue cultures thereof have scavenging effect on active oxygen [ J ] plant resource and environment science report, 2001(2) research on 5 kinds of dendrobium and tissue cultures thereof has found that dendrobium candidum has higher use value in traditional dendrobium medicinal materials, and the medicinal materials and the cultures thereof have higher capability of scavenging ROS. In order to further analyze and improve the related performance of dendrobium officinale, documents are as follows: the skin care effect research of the dendrobium officinale protocorm extract [ J ]. Guangzhou chemical engineering, 2020,48(04):90-92+132. active substances in the dendrobium officinale protocorm are extracted by adopting cold pressing, hot water extraction and ethanol extraction modes, and the chemical compositions, the antioxidant activity and the tyrosinase inhibition effect of corresponding water extract, alcohol extract and cold pressing are analyzed and compared. The patent CN202010441795.5 discloses a dendrobe extract, a preparation method and application thereof, the method specifically comprises the steps of flash extraction, high-pH high-concentration ethanol soaking, activated carbon decoloration, water extraction, enzymolysis, addition of propylene glycol and the like, the dendrobe extract with mild conditions and maximally protected effective components is prepared, and then the dendrobe extract is applied to cosmetics to exert the effects of moistening skin, inhibiting bacteria, removing acnes, diminishing inflammation and resisting allergy. In addition to the dendrobium extract, plant extracts such as various extracts including dendrobium stem extract, angelica extract, verbena extract and the like have been sufficiently researched and applied, wherein related researches on adding the plant extracts to a facial cleanser to exert the effects of the plant extracts are not lacked, for example, patent CN201711352414.0 discloses a facial cleanser containing dendrobium officinale extract and a preparation method thereof, and the invention discloses that the facial cleanser comprises the following specific components: the facial cleanser is characterized by comprising succinate surfactant, amino acid surfactant, zwitterionic surfactant, humectant, emulsifier, cetostearyl alcohol, sodium chloride, thickener, skin feel regulator, p-hydroxyacetophenone, 1, 2-hexanediol, alpine plant extract, dendrobium officinale extract and ascorbyl palmitate. However, according to recent research, the dendrobium officinale extract, the alpine plant extract and the like in the invention do not play greater advantages, the research on the face cleaning level is still lacked, meanwhile, only a plurality of performance tests are carried out in the invention, and only the pH value is investigated on the irritation research level, and other specific test tests are not carried out.

Aiming at the problem of relatively poor efficacy of the facial cleanser in the prior art, it is necessary to find a mild and non-irritant product which can fully realize the utilization of active components.

Disclosure of Invention

The invention provides the face cleanser containing the dendrobium officinale extract and the preparation method thereof, aiming at the problems in the prior art.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the invention provides a facial cleanser containing dendrobium officinale extract, which comprises a plant extract A, a plant extract B, a succinate surfactant, an amino acid surfactant, a zwitterionic surfactant, a humectant, an emulsifier, cetostearyl alcohol, sodium chloride, a thickening agent, a skin feel regulator, p-hydroxyacetophenone and 1, 2-hexanediol;

the plant extract A comprises dendrobium officinale extract and angelica keiskei extract;

the plant extract B comprises herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Adonidis extract.

Further, the facial cleanser comprises the following components in parts by weight: 0.5-3 parts of plant extract A, 0.3-1.2 parts of plant extract B, 30-45 parts of succinate surfactant, 10-25 parts of amino acid surfactant, 6-22 parts of zwitterionic surfactant, 8-20 parts of humectant, 0.1-0.5 part of emulsifier, 1-5 parts of cetearyl alcohol, 0.5-4 parts of sodium chloride, 0.5-5 parts of thickening agent, 0.5-5.5 parts of skin feel regulator, 0.6-0.7 part of p-hydroxyacetophenone and 0.3-1.1 part of 1, 2-hexanediol.

Preferably, the facial cleanser comprises the following components in parts by weight: 1-2 parts of plant extract A, 0.5-1 part of plant extract B, 35-40 parts of succinate surfactant, 15-20 parts of amino acid surfactant, 8-18 parts of zwitterionic surfactant, 10-18 parts of humectant, 0.1-0.4 part of emulsifier, 2-5 parts of cetostearyl alcohol, 1.5-4 parts of sodium chloride, 1-4 parts of thickener, 2-5 parts of skin feel regulator, 0.6-0.7 part of p-hydroxyacetophenone and 0.5-1 part of 1, 2-hexanediol.

Further preferably, the facial cleanser comprises the following components in parts by weight: 1.5 parts of plant extract A, 0.6 part of plant extract B, 38 parts of succinate surfactant, 18 parts of amino acid surfactant, 12 parts of zwitterionic surfactant, 12 parts of humectant, 0.2 part of emulsifier, 3 parts of cetearyl alcohol, 2.5 parts of sodium chloride, 3 parts of thickener, 3 parts of skin feel regulator, 0.6 part of p-hydroxyacetophenone and 0.6 part of 1, 2-hexanediol.

Further, the plant extract A is a dendrobium officinale extract and a angelica keiskei extract in a weight ratio of 3-9: 2.

Further, the plant extract B is a dendrobium nobile extract, a verbena extract, a wild rose extract and a snow lotus herb extract in a weight ratio of 3-5:1-2:1-2: 1.

Further, the succinate surfactant is disodium lauryl sulfosuccinate; the amino acid surfactant is one or more of sodium lauroyl sarcosinate, TEA (TEA cocoyl glutamate) salt and sodium lauroyl glutamate; the zwitterionic surfactant is cocamidopropyl betaine; the humectant is one or more of glycerol, diglycerol and polyethylene glycol-8; the emulsifier is methyl glucose sesquistearate; the thickening agent is acrylic acid (ester) copolymer; the skin feeling regulator is polyquaternium-7.

Further, the weight ratio of the plant extract A to the plant extract B is 2-3: 1.

The invention also provides a preparation method of the facial cleanser, which comprises the following steps:

(1) adding succinate surfactant, amino acid surfactant, zwitterionic surfactant, humectant, emulsifier and stearin into an emulsifying pot, heating and stirring, and keeping the temperature until the components are completely dissolved to obtain transparent liquid;

(2) mixing sodium chloride and water, adding the mixture into the transparent liquid obtained in the step (1) to obtain a mixture A, and cooling until the mixture A is solidified and uniform;

(3) adding p-hydroxyacetophenone and 1, 2-hexanediol into the mixture A at 60-70 ℃, cooling to 55-60 ℃, slowly adding the thickening agent, the water and the mixture while stirring, adding the mixture of the skin feel modifier and the water after uniformly stirring, and adding the plant extract A after uniformly stirring to obtain a mixture B;

(4) stirring plant extract B and ascorbyl palmitate at 50-55 deg.C, cooling to 40-45 deg.C, filtering, and discharging to obtain facial cleanser.

Further, the heating temperature in the step (1) is 85-90 ℃, and the heat preservation time is 20-40 min.

Further, the adding amount of water in the step (2) and the step (3) is 1-5 parts.

The technical effects obtained by the invention are as follows:

(1) according to the invention, the plant extract A (namely the dendrobium officinale extract and the angelica keiskei extract) and the plant extract B (namely the dendrobium nobile extract, the verbena extract, the wild rose extract and the snow lotus herb extract) are added into the facial cleanser, so that the synergistic effect of the dendrobium officinale extract and the angelica keiskei extract and other alpine plant extracts can be fully exerted, and meanwhile, the facial cleanser is mild in compounding and has strong washing capacity, and the disodium lauryl sulfosuccinate is used as a main surface active agent, so that the facial cleanser can mildly and efficiently clean the skin;

(2) the facial cleanser is prepared by compounding the plant extract, the surfactant, the humectant, the emulsifier, the cetearyl alcohol, the sodium chloride, the thickening agent and the skin feel regulator, and can effectively improve the skin glossiness, and the facial cleanser is mild, free of stimulation and good in decontamination effect.

Detailed Description

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.

Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention.

When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

In addition, the dendrobium officinale extract, the dendrobium nobile extract, the verbena extract and the snow lotus herb extract used in the invention are purchased from Shanxi New Tian territory Biotechnology Limited company, the angelica keiskei extract and the wild rose extract are purchased from Xiansafeao Biotechnology Limited company, and other raw materials are all common commercial products, so the sources of the raw materials are not particularly limited.

Example 1

The facial cleanser comprises the following components in parts by weight: 0.5 part of plant extract A, 0.3 part of plant extract B, 30 parts of succinate surfactant, 25 parts of amino acid surfactant, 6 parts of zwitterionic surfactant, 20 parts of humectant, 0.1 part of emulsifier, 1 part of cetearyl alcohol, 0.5 part of sodium chloride, 0.5 part of thickening agent, 0.5 part of skin feel modifier, 0.7 part of p-hydroxyacetophenone and 0.3 part of 1, 2-hexanediol. The plant extract A is a dendrobium officinale extract and a angelica keiskei extract in a weight ratio of 3: 2; the plant extract B is herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Adonidis extract at a weight ratio of 3:1:2: 1.

Wherein the succinate surfactant is disodium lauryl sulfosuccinate; the amino acid surfactant is sodium lauroyl sarcosinate; the zwitterionic surfactant is cocamidopropyl betaine; the humectant is glycerol; the emulsifier is methyl glucose sesquistearate; the thickening agent is acrylic acid (ester) copolymer; the skin feeling regulator is polyquaternium-7.

The preparation method of the facial cleanser comprises the following steps:

(1) adding succinate surfactant, amino acid surfactant, zwitterionic surfactant, humectant, emulsifier and stearin into an emulsifying pot, heating to 85 deg.C, stirring for 40min, and maintaining the temperature until the mixture is completely dissolved to obtain transparent liquid;

(2) mixing sodium chloride and 1 part of water, adding the mixture into the transparent liquid obtained in the step (1) to obtain a mixture A, and cooling until the mixture A is pasty uniformly;

(3) adding p-hydroxyacetophenone and 1, 2-hexanediol into the mixture A at 60 ℃, cooling to 55 ℃, slowly adding the thickening agent and 1 part of water and the mixture while stirring, adding the mixture of the skin feel modifier and 1 part of water after uniformly stirring, and adding the plant extract A after uniformly stirring to obtain a mixture B;

(4) and (3) at 50 ℃, uniformly stirring the plant extract B and the ascorbyl palmitate, cooling to 40 ℃, filtering and discharging to obtain the facial cleanser.

Example 2

The facial cleanser comprises the following components in parts by weight: 3 parts of plant extract A, 1.2 parts of plant extract B, 45 parts of succinate surfactant, 10 parts of amino acid surfactant, 22 parts of zwitterionic surfactant, 8 parts of humectant, 0.5 part of emulsifier, 5 parts of cetostearyl alcohol, 4 parts of sodium chloride, 5 parts of thickener, 5.5 parts of skin feel regulator, 0.6 part of p-hydroxyacetophenone and 1.1 part of 1, 2-hexanediol. The plant extract A is a dendrobium officinale extract and a angelica keiskei extract in a weight ratio of 9: 2; the plant extract B is herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Adonidis extract at a weight ratio of 5:2:1: 1.

Wherein the succinate surfactant is disodium lauryl sulfosuccinate; the amino acid surfactant is cocoyl glutamic acid TEA salt; the zwitterionic surfactant is cocamidopropyl betaine; the humectant is diglycerol; the emulsifier is methyl glucose sesquistearate; the thickening agent is acrylic acid (ester) copolymer; the skin feeling regulator is polyquaternium-7.

The preparation method of the facial cleanser comprises the following steps:

(1) adding succinate surfactant, amino acid surfactant, zwitterionic surfactant, humectant, emulsifier and stearin into an emulsifying pot, heating to 90 deg.C, stirring for 20min, and maintaining the temperature until the mixture is completely dissolved to obtain transparent liquid;

(2) mixing sodium chloride and 5 parts of water, adding the mixture into the transparent liquid obtained in the step (1) to obtain a mixture A, and cooling until the mixture A is pasty uniformly;

(3) adding p-hydroxyacetophenone and 1, 2-hexanediol into the mixture A at 70 ℃, cooling to 60 ℃, slowly adding the thickening agent and 5 parts of water and the mixture while stirring, adding the mixture of the skin feel modifier and 5 parts of water after uniformly stirring, and adding the plant extract A after uniformly stirring to obtain a mixture B;

(4) stirring plant extract B and ascorbyl palmitate at 55 deg.C, cooling to 45 deg.C, filtering, and discharging to obtain facial cleanser.

Example 3

The facial cleanser comprises the following components in parts by weight: 1.5 parts of plant extract A, 0.6 part of plant extract B, 38 parts of succinate surfactant, 18 parts of amino acid surfactant, 12 parts of zwitterionic surfactant, 12 parts of humectant, 0.2 part of emulsifier, 3 parts of cetearyl alcohol, 2.5 parts of sodium chloride, 3 parts of thickener, 3 parts of skin feel regulator, 0.6 part of p-hydroxyacetophenone and 0.6 part of 1, 2-hexanediol. The plant extract A is a dendrobium officinale extract and a angelica keiskei extract in a weight ratio of 5: 2; the plant extract B is herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Saussureae Involueratae extract at a weight ratio of 4:1.5:1.5: 1.

Wherein the succinate surfactant is disodium lauryl sulfosuccinate; the amino acid surfactant is sodium lauroyl glutamate; the zwitterionic surfactant is cocamidopropyl betaine; the humectant is polyethylene glycol-8; the emulsifier is methyl glucose sesquistearate; the thickening agent is acrylic acid (ester) copolymer; the skin feeling regulator is polyquaternium-7.

The above-mentioned face cleanser was prepared in the same manner as in example 1.

Example 4

The facial cleanser comprises the following components in parts by weight: the skin-care cream comprises, by weight, 1 parts of plant extract A, 1 parts of plant extract B, 40 parts of succinate surfactant, 20 parts of amino acid surfactant, 8 parts of zwitterionic surfactant, 18 parts of humectant, 0.1 part of emulsifier, 2 parts of cetearyl alcohol, 1.5 parts of sodium chloride, 1 part of thickener, 2 parts of skin-feel regulator, 0.6 part of p-hydroxyacetophenone and 0.5 part of 1, 2-hexanediol. The plant extract A is a dendrobium officinale extract and a angelica keiskei extract in a weight ratio of 3: 2; the plant extract B is dendrobium nobile extract, verbena extract, wild rose extract and snow lotus herb extract in a weight ratio of 3:2:2: 1.

Wherein the succinate surfactant is disodium lauryl sulfosuccinate; the amino acid surfactant is sodium lauroyl sarcosinate; the zwitterionic surfactant is cocamidopropyl betaine; the humectant is glycerol; the emulsifier is methyl glucose sesquistearate; the thickening agent is acrylic acid (ester) copolymer; the skin feeling regulator is polyquaternium-7.

The above-mentioned face cleanser was prepared in the same manner as in example 1.

Example 5

The facial cleanser comprises the following components in parts by weight: 2 parts of plant extract A, 0.5 part of plant extract B, 35 parts of succinate surfactant, 15 parts of amino acid surfactant, 18 parts of zwitterionic surfactant, 10 parts of humectant, 0.4 part of emulsifier, 5 parts of cetearyl alcohol, 4 parts of sodium chloride, 4 parts of thickener, 5 parts of skin feel regulator, 0.7 part of p-hydroxyacetophenone and 1 part of 1, 2-hexanediol. The plant extract A is a dendrobium officinale extract and a angelica keiskei extract in a weight ratio of 9: 2; the plant extract B is herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Adonidis extract at a weight ratio of 5:1:1: 1.

Wherein the succinate surfactant is disodium lauryl sulfosuccinate; the amino acid surfactant is cocoyl glutamic acid TEA salt; the zwitterionic surfactant is cocamidopropyl betaine; the humectant is diglycerol; the emulsifier is methyl glucose sesquistearate; the thickening agent is acrylic acid (ester) copolymer; the skin feeling regulator is polyquaternium-7.

The above-mentioned face cleanser was prepared in the same manner as in example 1.

Comparative example 1

The only difference from example 1 is that plant extract a is dendrobium officinale extract and angelica keiskei extract in a weight ratio of 1: 1; the plant extract B is herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Adonidis extract at a weight ratio of 2:3:0.8: 1.

Comparative example 2

The only difference from example 1 is that plant extract a is the dendrobium officinale extract and the angelica keiskei extract in a weight ratio of 5: 1; the plant extract B is herba Dendrobii extract, herba Verbenae extract, flos Rosae Davuricae extract and herba Adonidis extract at a weight ratio of 6:0.8:3: 1.

Comparative example 3

The only difference from example 1 is that the snow lotus herb extract was replaced with an equal amount of red peony root extract.

Comparative example 4

The facial cleanser of example 2 in patent CN 107854365A.

pH detection and skin irritation test

Test materials: SPF (specific pathogen free) eggs, purchased from New Yonghua farm poultry Co., Ltd (Wenshi group), were incubated until day 10 for testing, and incubation conditions were as follows: the room temperature is 20-25 ℃, the relative humidity is 45-70%, the incubation temperature is 37.5 +/-0.5%, the relative humidity is 55-70%, and the turntable frequency is 3 times/h-6 times/h.

The test method comprises the following steps:

(1) the pH value detection refers to GB/T13531.1, and the qualified standard range is 9.0-10.0.

(2) The test is carried out according to SNT2329-2009 cosmetic eye irritation/corrosiveness chick embryo appearance allantoic membrane test standard released by the industry standard of the entry and exit inspection epidemic of the people's republic of China, and the irritation capability of the sample is inspected.

The method specifically comprises the following steps: performing egg-candling inspection on 9-day-old chick embryos, and marking the positions of air chambers on the surfaces of the chick embryos; a small hole is carefully drilled above the air cell with a punch and dental forceps are used to peel off the marked portion of the eggshell exposing the white film. Sucking a proper amount of NaCl solution with the mass fraction of 0.9% by using a suction pipe to wet the egg membrane, and pouring out the solution. The intima is carefully removed with forceps to ensure that the vessel is not damaged. If the tested substance is transparent liquid, the method is carried out by adopting a time reaction experiment method. Respectively sucking 0.3mL of the test agent, dripping on the surface of chorioallantoic membrane, recording the change condition of blood vessel within 5min, observing the bleeding, thawing and blood coagulation time of blood vessel, and taking pictures and recording. Calculating the IS value of the stimulation score according to a formula, carrying out parallel experiments on each sample, calculating the average time of bleeding, blood melting and blood coagulation, and calculating the average IS value according to the formula.

IS＝(301-secH)×5/300+(301-secL)×7/300+(301-secC)×9/300

Among these, secH (bleeding time) -the mean time to onset of bleeding observed on the CAM membrane, unit s, secL (vascular thawing time) -the mean time to onset of vascular thawing observed on the CAM membrane, unit s, secC (clotting time) -the mean time to onset of clotting observed on the CAM membrane, unit s.

Bleeding: blood flows from the blood vessels and/or capillaries of the CAM and can take on a variety of manifestations such as cauliflower-like, diffuse and diffuse, or spot bleeding.

Blood coagulation: denaturation of both endo-and exo-proteins, usually seen only in the major and middle classes, does not include coagulation: denaturation of both endo-and exo-proteins, usually found only in large and medium-sized blood vessels, does not include changes that occur in capillaries.

Vessel melting: this refers to the disappearance of blood vessels from the CAM membrane, which may be due to a multifactorial change such as bleeding and vessel wall tension.

The test substance: the product in each example of the present invention was diluted to 1% by mass and evaluated by a reaction time method (irritation evaluation method). Two groups of control groups are additionally arranged, wherein one group is normal saline with the mass fraction of 0.9%, and the other group is K12 with the mass fraction of 1%. The number of the chick embryos tested in the control group and the chick embryos tested in each example group is respectively 6.

The evaluation of the results of the stimulus scoring method is shown in Table 1, and the test results of the test substances of the present invention are shown in Table 2:

TABLE 1

Stimulation scoring	Irritation classification
		IS＜1	Has no irritation
1≤IS＜5	Light irritation
		5≤IS＜9	Moderate irritation
IS≥10	Strong irritation/corrosion

TABLE 2

As can be seen from Table 1, the allantoic membrane IS stimulated by normal saline for 5min IS 0 and IS nonirritating, the allantoic membrane IS stimulated by K12 IS 13.08 after 5min and IS strongly irritating/corrosive, which indicates that the system IS successfully molded, and the test results of examples 1-5 show that the facial cleanser of each example IS qualified in terms of pH, and the facial cleanser in the invention IS milder and nonirritating according to the calculation of IS. In contrast, as shown in comparative examples 1 to 2, when the contents of the components were changed, the corresponding face creams were slightly irritating due to the change in the contents of the raw materials, due to the effect of the formulation level, especially in the case of comparative example 2. Comparative example 3 is the effect of individual component adjustment, and comparative example 4 shows the light irritation exhibited by the facial cleanser of the corresponding invention.

Second, clean force test

The test method comprises the following steps: since the test article of comparative example 2 was slightly irritant and had a relatively high IS value and simultaneously exhibited bleeding and blood coagulation, the group of comparative example 2 was shaved off and the remaining groups of face washes were tested as the test article. Selecting 40 healthy skin subjects, randomly dividing into 4 groups, each group comprising 10 subjects, testing two different examples of the subjects, respectively smearing 3cm × 1cm oily lipstick and eyebrow pencil on the inner side of the left arm and the right arm within 4cm × 5cm, respectively, smearing 0.5g of the subjects on the spots of the left arm and the right arm, gently rubbing for 1min, and washing with warm water.

And (4) judging the standard: photographing comparison before and after the test, grid counting, complete removal or nearly complete removal of lipstick and eyebrow pencil stains are effective, removal of more than 1/2 but obvious marks are still effective, no obvious removal effect is effective, and the results are counted in a table 3.

TABLE 3

As can be seen from the above table, the facial cleanser of the present invention has a clear cleansing power, and can achieve a clear removal of stains, compared to the relatively poor crystallization ability of the remaining groups.

Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.