阅读说明：本技术 一种液体样品拉曼光谱检测的制备方法及其设备 (A kind of preparation method and its equipment of the detection of fluid sample Raman spectrum ) 是由 白杨 邱录贵 于珍 于 2019-07-11 设计创作，主要内容包括：本发明涉及光谱检测技术,具体为一种液体样品拉曼光谱检测的制备方法及其设备,方法包括以下步骤：一、以展平的锡箔纸为基底降低拉曼散射的基底背景,并将锡箔纸固定在石英玻片上；二、采取定量的液体样本滴在锡箔纸之上,垂直点样,在保持周围温度处于室温下并静置固定的时间,静置的固定时间内液体样本干涸；三、对干涸的液体样本进行拉曼光谱的收集；四、更换锡箔纸,按照步骤二中设定的液体样本的定量、室温和静置时间,重复操作。本发明可成功替代铝箔,且样品量可控,实验体系稳定。(The present invention relates to spectrum detection techniques, the specially a kind of preparation method and its equipment of the detection of fluid sample Raman spectrum, method the following steps are included: one, reduce using the masking foil of flattening as substrate the substrate background of Raman scattering, and masking foil is fixed on quartz slide；Two, quantitative liquid sample is taken to drip on masking foil, vertical point sample is keeping ambient temperature to be at room temperature and standing regular time, and the set time inner sample liquid of standing is dry；Three, the collection of Raman spectrum is carried out to dry liquid sample；Four, masking foil is replaced, according to the quantitative of the liquid sample set in step 2, room temperature and time of repose, repetitive operation.The present invention can successfully substitute aluminium foil, and sample size is controllable, and experimental system is stablized.)

1. a kind of preparation method of fluid sample Raman spectrum detection, comprising the following steps:

One, with the masking foil of flattening (3) it is the substrate background of substrate reduction Raman scattering, and masking foil (3) is fixed on quartz On slide (1)；

Two, 4-10 microlitres of liquid sample (4) is taken to drip on masking foil (3), vertical point sample is keeping ambient temperature to be in Under 24-26 degrees Celsius and stand 20-60 minutes, 20-60 minutes inner sample liquid (4) it is dry；

Three, the collection of Raman spectrum is carried out to the liquid sample after drying up；

Four, masking foil is replaced, according to the quantitative of the liquid sample (4) set in step 2, room temperature and time of repose, repeats to grasp Make.

2. a kind of preparation method of fluid sample Raman spectrum detection according to claim 1, it is characterised in that: the step In rapid two, 5 microlitres of liquid sample (4) is taken to drip on masking foil (3).

3. a kind of preparation method of fluid sample Raman spectrum detection according to claim 1, it is characterised in that: the step In rapid two, liquid sample (4) in the case where keeping ambient temperature to be in 25 degrees Celsius and is stood 30 minutes, 30 minutes inner sample liquids (4) dry up.

It is set 4. being prepared used in the preparation method detected according to claim 1 to any one fluid sample Raman spectrum in 3 It is standby, it is characterised in that: including quartz slide (1), double-sided adhesive is equipped at the center of quartz slide (1) upper surface four edges (2), the masking foil (3) of flattening is adhesive on the double-sided adhesive (2), drop there are fluid sample (4) on the masking foil (3).

It is set 5. being prepared used in the preparation method detected according to claim 1 to any one fluid sample Raman spectrum in 3 It is standby, it is characterised in that: including quartz slide (1), quartz slide (1) two sides are equipped with paperweight fixed plate mating groove (51), It is equipped with paperweight fixed plate (5) in the paperweight fixed plate mating groove (51), on the inside of the paperweight fixed plate mating groove (51) Equipped with T-slot (61), T shape paperweight (6) is installed in the T-slot (61), passes through paperweight fixed plate mating groove (51) and paperweight Masking foil (3) flattening is fixed on quartz slide by the cooperation of fixed plate (5) and the cooperation of T-slot (61) and T shape paperweight (6) (1) on, drop has fluid sample (4) on the masking foil (3).

6. Preparation equipment used in a kind of preparation method of fluid sample Raman spectrum detection according to claim 5, It is characterized by: along setting, there are two fixed plate connector (521), the paperweight fixed plate mating grooves in the paperweight fixed plate (5) (51) fixed plate connector (521) is corresponded on inner surface is additionally provided with two fixed plate card slots (52).

7. Preparation equipment used in a kind of preparation method of fluid sample Raman spectrum detection according to claim 5, It is characterized by: the paperweight fixed plate (5) is set on the outside of T shape paperweight (6).

Technical field

The present invention relates to spectrum detection technique, preparation method and its set that specially a kind of fluid sample Raman spectrum detects It is standby.

Background technique

Raman spectrum is a kind of scattering spectrum, and principle is to be occurred when light passes through transparent medium by the light of molecular scattering Frequency variation can be analyzed the scattering spectrum different from incident light frequency whereby to obtain molecular vibration, rotation aspect Information, and adopting said method is studied in molecular structure.Nowadays, the application range of Raman spectrum spreads chemistry, physics, biology The every field such as and medicine have very big value for pure qualitative analysis, height quantitative analysis and measurement molecular structure.

But Raman spectrum itself is very faint, the introducing of any substance can all bring to a certain degree measured body system Pollution, this, which is equal to, improves a possibility that introducing some errors, and certain influence can be generated to the result of analysis.Therefore for liquid The Raman spectrum of sample detects, it is desirable that and the property and bearing basement of its liquid solvent do not generate interference to scattering spectrum, and one As water as its distracter very little of solvent, therefore the promotion item of the Raman spectrum detection technique of fluid sample be to carry sample it is molten The substrate of liquid.

Summary of the invention

The purpose of the present invention is to provide the preparation methods and its equipment of a kind of detection of fluid sample Raman spectrum, to solve The above problem.

To achieve the above object, the invention provides the following technical scheme:

A kind of preparation method of fluid sample Raman spectrum detection, comprising the following steps:

One, the substrate background of Raman scattering is reduced using the masking foil of flattening as substrate, and masking foil is fixed on quartz slide；

Two, 4-10 microlitres of liquid sample is taken to drip on masking foil, vertical point sample is keeping ambient temperature to be in 24-26 Under degree Celsius and 20-60 minutes are stood, inner sample liquid is dry within 20-60 minutes；

Three, the collection of Raman spectrum is carried out to the liquid sample after drying up；

Four, masking foil is replaced, according to the quantitative of the liquid sample set in step 2, room temperature and time of repose, repetitive operation.

Further, in the step 2,5 microlitres of liquid sample is taken to drip on masking foil.

Further, in the step 2, liquid sample in the case where keeping ambient temperature to be in 25 degrees Celsius and is stood 30 Minute, inner sample liquid is dry within 30 minutes.

Preparation equipment used in a kind of preparation method of fluid sample Raman spectrum detection, including quartz slide, it is described It is equipped with double-sided adhesive at the center of quartz slide upper surface four edges, the masking foil of flattening, the tin are adhesive on the double-sided adhesive Drop has fluid sample on foil paper.

Preparation equipment used in a kind of preparation method of fluid sample Raman spectrum detection, including quartz slide, it is described Quartz slide two sides are equipped with paperweight fixed plate mating groove, and paperweight fixed plate, institute are equipped in the paperweight fixed plate mating groove It states and is equipped with T-slot on the inside of paperweight fixed plate mating groove, T shape paperweight is installed in the T-slot, is cooperated by paperweight fixed plate Slot and the cooperation of paperweight fixed plate and the cooperation of T-slot and T shape paperweight, by being fixed on quartz slide for masking foil flattening, institute Stating drop on masking foil has fluid sample.

Further, in the paperweight fixed plate along setting there are two fixed plate connector, in the paperweight fixed plate mating groove Fixed plate connector is corresponded on surface is additionally provided with two fixed plate card slots.

Further, the paperweight fixed plate is set on the outside of T shape paperweight.

Beneficial effects of the present invention:

1, the masking foil used can be domestic level, and as the substitute of aluminium foil, cost is relatively low, and sample size is controllable, time ginseng Number is fixed, and experimental system is stablized, efficiently succinct in such a way that double-sided adhesive is fixed；

If 2, being convenient for changing substrate using the fixed masking foil of T shape paperweight, both sides also help flattening masking foil when fixed Face；

3, liquid sample, using the sample of small fixed volume, vertical point sample, the drying at room temperature set time is conducive to quantitative Repeat the expansion of experiment；

4, the oxidisability of tinfoil is lower than aluminium foil, and the result that the program obtains tests stable system.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of first embodiment in the present invention；

Fig. 2 is the structure sectional view of second embodiment in the present invention；

Fig. 3 is the quartz slide structural schematic diagram of second embodiment in the present invention；

Fig. 4 is the T shape paperweight structural schematic diagram of second embodiment in the present invention；

Fig. 5 is the paperweight fixed plate structure schematic diagram of second embodiment in the present invention.

In figure: 1, quartz slide；2, double-sided adhesive；3, masking foil；4, fluid sample；5, paperweight fixed plate；51, paperweight is fixed Plate mating groove；52, fixed plate card slot；521, fixed plate connector；6, T shape paperweight, 61, T-slot.

Specific embodiment

Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ", It is " thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside", " suitable The orientation or positional relationship of the instructions such as hour hands ", " counterclockwise " is to be based on the orientation or positional relationship shown in the drawings, merely to just In description the present invention and simplify description, rather than the equipment of indication or suggestion meaning or element must have a particular orientation, with Specific orientation construction and operation, therefore be not considered as limiting the invention.