阅读说明：本技术 一种从仙人掌中提取总黄酮的制备方法 (A method for extracting total flavone from radix et caulis Opuntiae Dillenii ) 是由 杨成东 于 2018-05-29 设计创作，主要内容包括：本发明公开了一种从仙人掌中提取总黄酮的制备方法,其特征在于以下步骤：1)取干燥仙人掌叶,研磨制成粗粉；2)取粗粉,加入无水乙醇100ml,超声3次,功率120~200w,每次45min,合并提取液,回收乙醇,得到仙人掌浸膏；3)提取：取仙人掌浸膏加入适量蒸馏水,搅匀,加入到层析柱中调节流速为5ml/min,用蒸馏水洗去杂质,以10~70%乙醇为流动相解吸附；4)回收乙醇,得总黄酮提取物,真空干燥即得总黄酮。本发明制备的总黄酮,产品纯度高,品质好,工艺方法简便易行,适用于工业化生产。(the invention discloses a preparation method for extracting total flavonoids from cactus, which is characterized by comprising the following steps: 1) grinding dry cactus leaves into coarse powder; 2) taking the coarse powder, adding 100ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 120-200 w for 45min each time, combining extracting solutions, and recovering the ethyl alcohol to obtain a cactus extract; 3) extraction: adding a proper amount of distilled water into the cactus extract, uniformly stirring, adding the cactus extract into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the cactus extract with distilled water to remove impurities, and desorbing the cactus extract by using 10-70% ethanol as a mobile phase; 4) recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone. The total flavone prepared by the method has high product purity, good quality, simple and easy process method, and is suitable for industrial production.)

1. A preparation method for extracting total flavonoids from cactus is characterized by comprising the following steps:

1) grinding dry radix et caulis Opuntiae Dillenii into coarse powder;

2) Taking the coarse powder, adding 100ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 120-200 w for 45min each time, combining extracting solutions, and recovering the ethyl alcohol to obtain a cactus extract;

3) Extraction: adding a proper amount of distilled water into the cactus extract, uniformly stirring, adding the cactus extract into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the cactus extract with distilled water to remove impurities, and desorbing the cactus extract by using 10-70% ethanol as a mobile phase;

4) Recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone.

2. The method for extracting total flavonoids from cactus according to claim 1, wherein the ultrasonic power in step 2) is 60-200W.

3. The preparation method for extracting total flavonoids from cactus according to claim 1, wherein the chromatographic column in the step 3) is prepared by AB-8 type macroporous resin and polyamide pretreatment, and the content of ethanol desorbed by a mobile phase is 10-70%.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of total flavonoids.

background

cactus is a plant of the cactus genus of the Cactaceae family (Cactaceae), with approximately 108, nearly 2000 species worldwide, distributed mainly in central and south america, eastern africa, magastra, marshmallow and srilanca. Most of the genus species of the family are introduced to other regions in the world, more than 60 genera, about 600 species, are introduced and cultivated in China, and the cactus is the cactus with the most economic and medicinal value in cactaceae plants. The cactus has early application in the traditional Chinese medicine, is bitter and cool in nature, and has the effects of clearing heat and removing toxicity, relieving pain and swelling, invigorating stomach and relieving pain and the like; in addition, modern pharmacological research also proves that the cactus has the effects of resisting bacteria, diminishing inflammation, reducing blood sugar, resisting ulcer, resisting cancer, resisting oxidation, resisting virus, improving the immunity of the organism and the like. The main active ingredients in cactus are alkaloids, flavonoids, sterols, polysaccharides and the like, wherein the flavonoids are one of the main functional components and play an important role in playing a plurality of pharmacological actions.

In recent years, an ultrasonic extraction method is widely applied to extraction of effective components in natural plants as an excellent extraction method, and compared with a Soxhlet extraction method, a reflux extraction method and an ultrasonic extraction method for extracting total flavonoids, the result shows that the ultrasonic extraction method is higher in extraction rate than the Soxhlet extraction method and the reflux extraction method, and is shorter in time, so that the ultrasonic extraction method is more effective and simpler in method for extracting the total flavonoids.

Disclosure of Invention

the invention aims to provide a method for extracting total flavonoids, and the total flavonoids have wide medicinal and health-care values.

The invention is completed by the following technical scheme, and the specific content comprises the following steps: method for extracting total flavone

1) Grinding dry radix et caulis Opuntiae Dillenii into coarse powder;

2) Taking the coarse powder, adding 100ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 120-200 w for 45min each time, combining extracting solutions, and recovering the ethyl alcohol to obtain a cactus extract;

3) Extraction: adding a proper amount of distilled water into the cactus extract, uniformly stirring, adding the cactus extract into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the cactus extract with distilled water to remove impurities, and desorbing the cactus extract by using 20-85% ethanol as a mobile phase;

4) Recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone.

The power of the ultrasonic wave in the step 2) is 60-200W.

the chromatographic column in the step 3) is prepared by pretreating AB-8 type macroporous resin and polyamide, and the content of the mobile phase desorption ethanol is 10-70%.

the invention has the advantages that

The invention adopts ultrasonic extraction-AB-8 type macroporous resin and polyamide column chromatography to extract the total flavone in the cactus, the sample capacity of the column chromatography is large, the extraction efficiency is high, and the method is simple and easy to implement.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

the specific implementation mode is as follows: