阅读说明：本技术 一种微流体芯片加工方法 (Microfluid chip processing method ) 是由 翁岳衡 于 2021-08-17 设计创作，主要内容包括：本发明涉及一种微流体芯片加工方法,包括下述操作步骤：a.材料选取；b.材料清洗；c.材料剪裁；d.材料检测；e.通道加工；f.功能结构加工；g.洗脱残渣；h.其他功能加工；i.等比缩小；j.测量检测；k.包装；所述方法采用热缩片作为加工材料,通过选取表面需要光滑无裂纹、刮痕等的材料,通过雕刻、打磨、电镀、印刷等方法加工获得具有各种功能的微流控芯片。本发明的有益效果是：该微流体芯片加工方法,利用热缩片加热缩小的固有特性以较低成本较高误差的方法加工高精度的微流控芯片,实现了更低成本的热缩片微流控芯片开发与生产,热缩片微流控芯片的制备成功,意味着微流控芯片在价格上与大型仪器的对比中逐渐产生优势。(The invention relates to a microfluid chip processing method, which comprises the following operation steps: a. selecting materials; b. cleaning materials; c. cutting the material; d. detecting materials; e. processing a channel; f. processing a functional structure; g. eluting the residue; h. processing other functions; i. scaling down in equal proportion; j. measuring and detecting; k. packaging; the method adopts the thermal shrinkage sheet as a processing material, selects the material with smooth surface without cracks, scratches and the like, and processes the material by methods of carving, polishing, electroplating, printing and the like to obtain the microfluidic chip with various functions. The invention has the beneficial effects that: according to the method for processing the microfluidic chip, the high-precision microfluidic chip is processed by a method with low cost and high error by utilizing the inherent characteristic of heating shrinkage of the heat shrinkage sheet, development and production of the heat shrinkage sheet microfluidic chip with low cost are realized, and the successful preparation of the heat shrinkage sheet microfluidic chip means that the microfluidic chip gradually generates advantages in comparison with a large instrument in price.)

1. A microfluidic chip processing method is characterized by comprising the following operation steps: a. selecting materials; b. cleaning materials; c. cutting the material; d. detecting materials; e. processing a channel; f. processing a functional structure; g. eluting the residue; h. processing other functions; i. scaling down in equal proportion; j. measuring and detecting; k. packaging;

selecting materials: selecting a thermal shrinkage sheet as a processing material, and selecting a material with a smooth surface without cracks, scratches and the like.

2. A microfluidic chip processing method according to claim 1, wherein b. material cleaning: the selected material is placed in a special washing solution for washing, and then the material is dried.

3. A microfluidic chip fabrication method according to claim 1, wherein said c.material cutting: the material is cut by a cutting machine to a specified size.

4. The microfluidic chip processing method according to claim 1, wherein the d. material detection: and selecting leftover materials intercepted from the materials, and then detecting the leftover materials by using a detection machine.

5. A microfluidic chip processing method according to claim 1, wherein said e-channel processing: and machining the designed microfluidic structure on the material by a CNC engraving method and the like.

6. The microfluidic chip processing method according to claim 1, wherein the f-functional structure processing: and (4) processing an easily-modified rough surface, a conductive wire accommodating groove or other structures on the material by grinding by a grinding machine, CNC (computerized numerical control) engraving or other methods.

7. A microfluidic chip processing method according to claim 1, wherein the h.elution residue: the finished material is then placed in a special machine for residue elution.

8. The microfluidic chip processing method of claim 1, wherein the i.e. geometric scale down: and heating the processed material and matching with the sample channel and the upper cover to obtain a micro-fluid structure with reduced equal ratio.

9. The microfluidic chip processing method according to claim 1, wherein the j-measurement detection: and measuring the finished product after the equipment is processed by using a miniature measuring instrument so as to determine whether the finished product is qualified or not.

10. The microfluidic chip processing method according to claim 1, wherein the k-pack: and (4) mounting or fixing the qualified finished product to a specified position, and packaging the finished product by a packaging machine.

Technical Field

The invention relates to the technical field of microfluidic electrophoresis chips, in particular to a microfluidic chip processing method.

Background

In life science or chemical experiments, physical and chemical operations on fluids are often performed, and therefore, a laboratory should have a centrifuge, a shaker, a PCR instrument, an electrophoresis instrument and other various experimental instruments, and needs to perform operations such as moving, mixing and the like on fluids in different equipment, but the laboratory is inconvenient when an obtained sample is extremely rare (measured in microliter or nanoliter) or expensive. The micro-fluidic chip made of silicon or silicon dioxide is the most mature and widely used technology, can be processed by common plane processing technologies such as precision photoetching, etching and the like, and can also be prepared by micromachining only through organic materials.

The micro-fluidic chip generally used at present depends on photoetching, the general principle of photoetching is to reduce and project patterns on a substrate in equal proportion through a series of optical systems, photosensitive resist coated on the substrate is subjected to photosensitive reaction to form the patterns on the substrate, parts needing etching are exposed after elution, and channels are formed at the parts which are not masked by the photosensitive resist under the action of an etchant to obtain a target product. Due to the need of processing micron-sized channels, photoetching is almost irreplaceable, the popularity of the microfluidic electrophoresis chip of a silicon dioxide or silicon carrier is limited due to the customized or purchased price, and the polyacrylamide electrophoresis equipment has the problems of low efficiency, high material consumption, complex operation, certain carcinogenicity of dyeing materials used in the operation and poor repeatability compared with the microfluidic chip electrophoresis.

It is therefore desirable to devise a microfluidic chip fabrication method that addresses the above-mentioned problems.

Disclosure of Invention

The invention aims to provide a microfluidic chip processing method, which aims to solve the problems that the prior commonly used microfluidic chip is totally dependent on photoetching in the background technology, the general principle of photoetching is that patterns are projected on a substrate in an equal proportion and reduced scale through a series of optical systems, photosensitive resist coated on the substrate is subjected to photosensitive reaction to form the patterns on the substrate, parts needing etching are exposed after elution, and channels are formed in the parts which are not masked by the photosensitive resist under the action of an etchant to obtain a target product. Due to the need of processing micron-sized channels, photoetching is almost irreplaceable, the popularity of the microfluidic electrophoresis chip of a silicon dioxide or silicon carrier is limited due to the customized or purchased price, and the polyacrylamide electrophoresis equipment has the problems of low efficiency, high material consumption, complex operation, certain carcinogenicity of dyeing materials used in the operation and poor repeatability compared with the microfluidic chip electrophoresis.

In order to achieve the purpose, the invention provides the following technical scheme: a microfluidic chip processing method comprises the following operation steps: a. selecting materials; b. cleaning materials; c. cutting the material; d. detecting materials; e. processing a channel; f. processing a functional structure; g. eluting the residue; h. processing other functions; i. scaling down in equal proportion; j. measuring and detecting; k. packaging;

selecting materials: selecting a thermal shrinkage sheet as a processing material, and selecting a material with a smooth surface without cracks, scratches and the like.

B, material cleaning: the selected material is placed in a special washing solution for washing, and then the material is dried.

C, material cutting: the material is cut by a cutting machine to a specified size.

The material detection: and selecting leftover materials intercepted from the materials, and then detecting the leftover materials by using a detection machine.

Preferably, the e-channel machining: and machining the designed microfluidic structure on the material by a CNC engraving method and the like.

Preferably, the f. functional structure processing: and (4) processing an easily-modified rough surface, a conductive wire accommodating groove or other structures on the material by grinding by a grinding machine, CNC (computerized numerical control) engraving or other methods.

Preferably, the h. elution residue: the finished material is then placed in a special machine for residue elution.

Preferably, the i.iso-scaling reduces: and heating the processed material and matching with the sample channel and the upper cover to obtain a micro-fluid structure with reduced equal ratio.

Preferably, the i.iso-scaling reduces: and heating the processed material and bonding the upper cover to obtain the micro-fluid channel with reduced equal ratio.

Preferably, the j measurement detection: and measuring the finished product after the equipment is processed by using a miniature measuring instrument so as to determine whether the finished product is qualified or not.

And k, packaging: and then, mounting or fixing the qualified finished product to a specified position, and packaging the finished product by a packaging machine.

Compared with the prior art, the invention has the beneficial effects that:

1. the shrinkage characteristic of the heat-shrinkable sheet is utilized to manufacture the heat-shrinkable sheet micro-fluidic hole electrophoresis chip to replace the conventional micro-fluidic electrophoresis chip which takes silicon dioxide or silicon as an etching substrate, the inherent characteristic of heat shrinkage of the heat-shrinkable sheet is utilized to process the high-precision micro-fluidic chip by a method with lower cost and higher error, the development and the production of the heat-shrinkable sheet micro-fluidic chip with lower cost are realized under the condition of ensuring the function of the micro-fluidic chip, and the successful preparation of the heat-shrinkable sheet micro-fluidic chip from the cost perspective means that the micro-fluidic chip gradually generates advantages in comparison with a large instrument in price, thereby being more popularized and practical in the market.

Detailed Description

The invention provides a technical scheme that: a microfluidic chip processing method comprises the following operation steps: selecting a thermal shrinkage sheet as a processing material, and selecting a material with a smooth surface without cracks, scratches and the like. The selected material is placed in a special washing solution for washing, and then the material is dried. The material is cut by a cutting machine to a specified size. And selecting leftover materials intercepted from the materials, and then detecting the leftover materials by using a detection machine. And machining the designed microfluidic structure on the material by a CNC engraving method and the like. And processing the rough surface, the conductive wire placing groove or other structures which are easy to modify on the material by grinding machine grinding, CNC engraving or other methods. The finished material is then placed in a special machine for residue elution. And heating the processed material and matching with the sample channel and the upper cover to obtain a micro-fluid structure with reduced equal ratio. And measuring the finished product after the equipment is processed by using a miniature measuring instrument so as to determine whether the finished product is qualified or not. And finally, packaging the finished product by a packaging machine.

The shrinkage characteristic of the heat-shrinkable sheet is utilized to manufacture the heat-shrinkable sheet micro-fluidic hole electrophoresis chip to replace the conventional micro-fluidic electrophoresis chip which takes silicon dioxide or silicon as an etching substrate, the inherent characteristic of heat shrinkage of the heat-shrinkable sheet is utilized to process the high-precision micro-fluidic chip by a method with lower cost and higher error, the development and the production of the heat-shrinkable sheet micro-fluidic chip with lower cost are realized under the condition of ensuring the function of the micro-fluidic chip, and the successful preparation of the heat-shrinkable sheet micro-fluidic chip from the cost perspective means that the micro-fluidic chip gradually generates advantages in comparison with a large instrument in price, thereby being more popularized and practical in the market.

The microfluidic chip is focused by many scholars in the biological field, and has many researches and applications, for example, a real-time PCR device controlled by a microfluidic system in the research of wujia et al, and the scholars try to use the microfluidic chip as a main structure in PCR isothermal amplification, etc., the microfluidic chip is widely used in PCR amplification at present, in the detection, the microfluidic chip can capture and analyze tumor cells, can rapidly and effectively screen the tumor cells, and the operations of separating and capturing other single cells in the microfluidic chip have certain possibility, which may cause the cell-level biological research to obtain breakthrough progress in the future, in the detection of new corona viruses, the microfluidic technology and the microfluidic chip are mature, and scholars such as shanxia make a detailed explanation on the aspect, except in the biological field, the microfluidic chip is applied to printing technologies such as liquid drop printing, biological three-dimensional printing and the like, and plays an important role in other fields such as food engineering, environmental detection, criminal military and the like; when the heat shrinkable sheet micro-fluidic chip is used for a highly integrated chip, the required channels are arranged in and out very many channels according to the following method: when the diameter of the pipeline is known (whether plastic or metal), a through hole in interference fit can be machined according to a pre-obtained shrinkage rate, the shrinkage rate is 1.85/1, so that after a through hole with the diameter of 1.9mm is machined in a heat-shrinkable sheet and a needle with the outer diameter of 1.1mm is inserted, stable connection can be obtained through heat shrinkage, a raised platform body can be formed around the needle due to the fact that wrinkles can occur after the heat shrinkage is hindered, the connection is quite tight, but the wrinkles can damage the shape of the channel, the sample liquid port and the functional area are far away from each other during design, the channel can be machined inexpensively and stably, and the channel such as a glass through window or other functional parts can be easily embedded by the same method.

The working principle is as follows: selecting a heat-shrinkable sheet as a processing material, processing a rough surface on the material by CNC engraving or other methods, processing a rough surface before non-shrinking due to the heat-shrinkable characteristic of the heat-shrinkable sheet, performing silk-screen printing, printing (practice proves that the printing method is the simplest and practical in small-batch production), even manually drawing patterns on the surface of the heat-shrinkable sheet by using graphite powder, nano silver or conductive ink, fixing the patterns to form electrodes or circuits after heat-shrinking, having good biocompatibility due to the inertia of graphite, being particularly suitable for processing various electrodes directly contacting reagents, for longer or parts of circuits with higher requirements on resistance, processing grooves with proper width and leading to copper wires (for example, processing a 0.2mm groove on the copper wire to leading to a 0.17mm copper wire or enameled wire), and tightly embedding the copper wires into a support plate during heat-shrinking to form a circuit with low resistance and high insulation, if the enameled wire is inlaid, even multi-kilovolt high-voltage electrophoresis can be carried out, a plurality of microfluidic chips which need prefabricated immobilized reagents exist, for example, certain antibodies or other bioactive substances are fixed on the inner surface to carry out enrichment work, certain substances can be stably fixed on a carrier plate by utilizing the shrinkage characteristic of a heat-shrinkable sheet, or surfaces with different roughness are processed to realize certain specific hydrodynamic functions, in the application of the microfluidic chip, the functions including centrifugation, gradient sample introduction, mixing and the like are often realized through certain specific channel structures, when the microfluidic chip is processed by using the heat-shrinkable sheet, in addition to the functions, a tiny three-dimensional structure can be manufactured by utilizing the shrinkage and elasticity of the heat-shrinkable sheet, for example, a scraper with a certain size is used for processing the heat-shrinkable sheet into larger burrs, and after the heat shrinkage, the burrs are sharply reduced and form barbs along the processing direction, the processed surface is distributed with fine barbs, T cells can be easily enriched, elution is easy to be carried out by reverse flow, or large and dense blind holes are processed, and when the blind holes are contracted, honeycomb-shaped deep blind holes (which are difficult to realize by a photoetching method) are formed in a channel and can be used for precipitation separation or drug slow release.