阅读说明：本技术 一种药用茶原料的灭菌方法、药用茶原料及其制备方法 (Sterilizing method of medicinal tea raw material, medicinal tea raw material and preparation method thereof ) 是由 李亦武 于 2017-12-28 设计创作，主要内容包括：本发明公开了一种药用茶原料的灭菌方法、药用茶原料及其制备方法,涉及茶剂加工方法领域。本发明将干燥后代用茶原料导入流化床、臭氧发生器、臭氧检测器和储气仓组成的密闭回路,使代用茶原料在密闭环境的臭氧气流中循环流动消毒,将风选、灭菌、干燥三道工序有机结合起来,满足了药用茶加工特定的灭菌需求。(The invention discloses a sterilization method of medicinal tea raw materials, the medicinal tea raw materials and a preparation method thereof, and relates to the field of tea preparation processing methods. The invention introduces the dried tea raw material into a closed loop consisting of a fluidized bed, an ozone generator, an ozone detector and a gas storage bin, so that the tea raw material is disinfected in a circulating flow manner in the ozone airflow in the closed environment, and the three procedures of winnowing, sterilization and drying are organically combined, thereby meeting the specific sterilization requirement of the processing of the medicinal tea.)

1. A sterilization method of medicinal tea raw materials is characterized by comprising the following steps:

sequentially connecting a fluidized bed, an ozone generator and a gas storage bin, connecting the fluidized bed with a material outlet of a filtering device, setting a material and gas circulation channel into a closed loop, and arranging an ozone detector in the closed loop;

starting an ozone generator, and controlling the concentration of ozone gas in the closed loop to be between 15 ppm and 25ppm and keeping the ozone gas stable;

introducing the dried medicinal tea raw material into a fluidized bed in a closed loop, and sterilizing the medicinal tea raw material in an ozone airflow in a closed environment in a circulating flow manner.

2. The sterilization method according to claim 1, wherein the material ratio of the ozone to the medicinal tea raw material is 1.2-1.6g:100 kg.

3. A sterilization process according to claim 1, wherein the fluidized bed is rotated at a speed of 70-90 r/min.

4. A sterilization process according to claim 1 wherein one or more of pressure, flow rate, pH, ozone generation rate in the closed circuit is controlled by closed loop computer control to maintain predetermined sterilization conditions.

5. The sterilization method according to claim 1, wherein the time for sterilization is 25 to 40 minutes.

6. The sterilization method according to any one of claims 1 to 5, the medicinal tea raw materials comprise one or more of poria cocos, liquorice, wolfberry, kudzu root, cassia seed, ginger, red tangerine peel, momordica grosvenori, pawpaw, Chinese yam, sea buckthorn, mulberry leaf, mulberry, polygonatum, jujube, chrysanthemum, hawthorn, gardenia, jasmine, mint, honeysuckle, lotus seed, lemon, chicory, sweet osmanthus flower, rose with double petals, orange peel, platycodon grandiflorum, maca powder, moringa oleifera leaf, perilla seed, barley, orange peel, hemp seed, aloe vera gel, wolfberry, scaphium scaphigerum, hawthorn, jasmine, lily, dandelion, clove, mint, lotus leaf, longan pulp, lotus seed, fingered citron, gardenia, angelica dahurica, elsholtzia, guava, pumpkin seed, ginseng, barley, brown rice, lotus leaf, mulberry leaf, fragrant solomonseal rhizome, rhizoma polygonati, dried ginger, purslane, lophatherum gracile, cinnamon, black sesame and nutmeg.

7. A preparation method of medicinal tea raw materials is characterized by comprising the following steps:

selecting fresh medicinal tea raw materials, respectively removing required rhizome, bud leaves, flowers, buds or parts except fruits according to the types of the medicinal tea raw materials, and cleaning with clear water for later use;

pulverizing the cleaned medicinal tea raw material into powder with average diameter of 250-;

mixing the pulverized medicinal tea raw materials, passing through a 40-60 mesh filter screen, and drying with 50 deg.C hot air during the filtering process;

the sterilization method according to any one of claims 1 to 5, wherein the medicinal tea raw material is sterilized by circulating flow in an ozone airflow in a closed environment;

subpackaging the sterilized medicinal tea raw materials into bags.

8. The method for preparing a medicinal tea material as set forth in claim 7, wherein: the drying is carried out by blowing hot air out of an air outlet pipe of the butt joint dust remover.

9. The method for preparing a medicinal tea material as set forth in claim 7, wherein: the colony numbers of staphylococcus aureus, shigella, salmonella and escherichia coli and the total colony number of the sterilized medicinal tea raw material were measured.

10. A medicinal tea material obtained by the production method according to any one of claims 7 to 9.

Technical Field

The invention relates to the field of tea processing methods, in particular to a sterilization method of medicinal tea raw materials, the medicinal tea raw materials and a preparation method thereof.

Background

In the traditional medicinal tea processing process, the working procedures of air separation, sterilization, drying and the like are necessary steps, but the working procedures can be carried out step by step only, so that the time and labor are wasted, and the working efficiency is lower.

In recent years, a new process integrates the three steps to form a linkage unmanned production line, so that the requirement of modern industrial production is met, but the requirements of the integrated new process on the separate steps of air separation, sterilization and drying are further improved. At present, the air separation and drying process and equipment in the related linkage unmanned production line are relatively mature, but the sterilization process still has a plurality of defects.

Common sterilization modes include dry heat sterilization, wet heat sterilization, 60Co radiation sterilization, ultraviolet sterilization, ozone sterilization and the like. However, the related technology still has a plurality of limitations when applied to sterilization of medicinal tea raw materials (including tea, medicinal and edible traditional Chinese medicinal materials and the like): heat sterilization relies on thermal effects and is not suitable for most heat sensitive ingredients; when the radiation dose is controlled improperly, the radiation can cause the damage, color change and flavor change of effective components, even possibly has radiation residue, and the radiation equipment is complex, has high protection requirement, high cost and difficult maintenance and other adverse factors, so the radiation equipment is difficult to be applied in large scale in actual production. Therefore, ozone sterilization becomes the first choice for sterilization of medicinal tea.

Medicinal tea raw materials are based on between medicinal material sliced medicinal herbs and the powder, and two kinds of ozone equipment of ozone sterilization cabinet and ozone bipyramid mixer that are general at present all can not reach ideal sterilization effect: the ozone sterilization cabinet is characterized in that materials are laid in a tray and placed in the cabinet, and ozone is introduced into the cabinet for sterilization, so that the ozone is difficult to fully contact with the materials, and the sterilization effect is not ideal; the ozone double-cone mixer is characterized in that materials and ozone are added into the mixer at the same time, and the materials and the ozone are in full contact through continuous overturning, but the ozone double-cone mixer has many problems in ozone concentration control and workshop protection, and is difficult to put into large-scale production and use.

Therefore, a brand new medicinal tea processing technology design is urgently needed to be designed, three procedures of air separation, sterilization and drying are organically combined, and the specific sterilization requirement of medicinal tea processing is met.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a method for sterilizing medicinal tea raw materials, the medicinal tea raw materials and a preparation method thereof, wherein three processes of air separation, sterilization and drying are organically combined, and the specific sterilization requirement of medicinal tea processing is met.

In order to achieve the above purpose, the invention provides a method for sterilizing medicinal tea raw materials, which comprises the following steps:

sequentially connecting a fluidized bed, an ozone generator and a gas storage bin, connecting the fluidized bed with a material outlet of a filtering device, setting a material and gas circulation channel into a closed loop, and arranging an ozone detector in the closed loop;

starting an ozone generator, and controlling the concentration of ozone gas in the closed loop to be between 15 ppm and 25ppm and keeping the ozone gas stable;

introducing the dried medicinal tea raw material into a fluidized bed in a closed loop, and sterilizing the medicinal tea raw material in an ozone airflow in a closed environment in a circulating flow manner.

Preferably, the material ratio of the ozone to the medicinal tea raw material is 1.2-1.6g:100 kg.

Preferably, the rotating speed of the fluidized bed is 70-90 r/min.

Preferably, one or more of pressure, flow rate, pH, ozone generation rate in the closed loop are controlled by closed loop computer control to maintain predetermined disinfection conditions.

Preferably, the time for sterilization is 25 to 40 minutes.

Preferably, the medicinal tea raw materials include poria cocos, liquorice, wolfberry, kudzu root, cassia seed, ginger (ginger, dried ginger), exocarpium citri rubrum, momordica grosvenori, pawpaw, Chinese yam, sea buckthorn, mulberry leaf, mulberry, polygonatum, jujube (jujube, wild jujube, black jujube), chrysanthemum, hawthorn, gardenia, jasmine, mint, honeysuckle, lotus seed, lemon, chicory, sweet osmanthus, rose, orange peel, platycodon grandiflorum, maca powder, moringa oleifera leaf, perilla seed, barley, orange peel, hemp seed, aloe vera gel, wolfberry, scaphium scaphigerum, hawthorn, jasmine, lily, dandelion, clove, mint, lotus leaf, longan pulp (longan), lotus seed, fingered citron, gardenia, angelica root, elsholtzia, guava, pumpkin seed, ginseng (artificially planted), barley, brown rice, lotus leaf, mulberry leaf, fragrant solomonseal, bitter orange flower, sealwort, dried ginger, purslane, lophatherum, cinnamon, mulberry, dried ginger, and the like, One or more of black sesame and nutmeg.

The invention also provides a preparation method of the medicinal tea raw material, which comprises the following steps:

selecting fresh medicinal tea raw materials, respectively removing required rhizome, bud leaves, flowers, buds or parts except fruits according to the types of the medicinal tea raw materials, and cleaning with clear water for later use;

crushing the cleaned medicinal tea raw material into powder with the average diameter of 250-350 mu m;

mixing the pulverized medicinal tea raw materials, passing through a 40-60 mesh filter screen, and drying with 50 deg.C hot air during the filtering process;

by using the sterilization method, the medicinal tea raw material circularly flows in the ozone airflow in the closed environment for sterilization;

subpackaging the sterilized medicinal tea raw materials into bags.

Preferably, the drying is performed by blowing hot air out of an air outlet pipe of the butt joint dust remover.

Preferably, the sterilized medicinal tea material is measured for the number of colonies of staphylococcus aureus, shigella, salmonella, and escherichia coli, and the total number of colonies.

The invention also provides a medicinal tea raw material obtained by using the preparation method.

Compared with the prior art, the invention has the advantages that:

(1) according to the processing method of the medicinal tea raw material, the ozone fluidized bed is used as a disinfection step device to kill microorganisms to replace the irradiation sterilization step in the prior art, so that the problem of irradiation residue in the existing irradiation sterilization method is solved; meanwhile, the winnowing, sterilizing and drying devices for processing the medicinal tea are organically combined, the ozone is fully contacted with the materials, the sterilizing effect is more ideal, and the processing cost of the medicinal tea raw materials is reduced.

(2) The processing method of the medicinal tea raw material uses fluidized bed drying to replace a thermal cycle air box drying step, reduces the loss of active ingredients of the medicinal tea raw material in the processing process, improves the drying efficiency of the material, and shortens the production period.

(3) The medicinal tea raw material solves the problem that fine powder of the crushed medicinal tea raw material affects the color of tea soup, and the tea color is clear and bright.

Detailed Description

The present invention will be described in further detail with reference to examples.

The definition and execution standard of the substitute tea set forth in the patent application document are set according to the description and requirements in GH/T1091-2014 substitute tea.

The fresh substitutive tea raw material used in the application document of the invention comprises the products which are prepared by taking plant bud leaves, flowers and buds, fruits, rhizomes and the like which are published by the administrative department of China and can be used for food, besides tea, as raw materials, and are drunk by people by processing and preparing the raw materials and adopting a mode similar to tea brewing or boiling. The substitute tea raw material which can be used in the processing method of the substitute tea raw material of the present invention includes, but is not limited to, poria cocos, licorice, wolfberry, pueraria, cassia seed, ginger (ginger, dried ginger), chrysoidine, momordica grosvenori, papaya, yam, sea buckthorn, mulberry leaf, mulberry, polygonatum, jujube (jujube, zizyphus jujube, chrysanthemum, hawthorn, gardenia, jasmine, peppermint, honeysuckle, lotus seed, lemon, chicory, sweet osmanthus, rose of heavy petal, orange peel, platycodon grandiflorum, maca powder, moringa oleifera leaf, perilla seed, barley, orange peel, hemp seed, aloe vera gel, wolfberry, boat-fruited sterculia, hawthorn, jasmine, lily, dandelion, clove, mint, lotus leaf, longan pulp (longan), lotus leaf, fingered citron, gardenia, angelica dahurica, elsholtzia, guava, pumpkin seed, ginseng (artificial plantation), barley, mulberry leaf, polygonatum, brown rice, substitute flowers, polygonatum, and other plants, One or more of Zingiberis rhizoma, herba Portulacae, folium Bambusae, cortex Cinnamomi, semen Sesami Niger, and semen Myristicae.

The scheme of the invention has the main advantages of utilizing ozone for sterilization: ozone is gas, so that the diffusivity is good, no dead angle exists, and the concentration distribution is uniform; the sterilizing capability is strong, the ozone sterilizing capability is equivalent to that of peroxyacetic acid and higher than that of other disinfectants; is suitable for various pathogenic microorganisms, and has good killing effect on various microorganisms such as escherichia coli, salmonella, staphylococcus aureus, hepatitis A virus, hepatitis B virus, fungi and the like; meanwhile, the ozone is prepared by utilizing the atmosphere around us, and storage facilities are not needed; ozone can be rapidly decomposed into oxygen and monatomic oxygen, and the monatomic oxygen can be combined into oxygen molecules, so that the problem of secondary pollution is avoided, and the ozone disinfectant is known as a green disinfectant.

The embodiment of the invention provides a processing method of a substitutional tea raw material, which comprises the following steps:

s1, cleaning: selecting fresh substitutional tea raw materials, respectively removing required rootstocks, bud leaves, flowers, buds and parts except fruits according to the varieties of the substitutional tea raw materials, and cleaning the fresh substitutional tea raw materials with clear water for standby;

s2, crushing: crushing various substituted tea raw materials treated by S1 into powder with the average diameter of 250-350 mu m;

s3, batching: mixing the crushed various substitutional tea raw materials;

s4, removing powder and drying: making the raw materials prepared in the step S3 pass through a filter screen of 40-60 meshes, wherein if the mesh number of the filter screen is too high, the powder of the substituted tea raw materials is too fine, so that the color and luster of the tea soup are influenced; too much impurities can be mixed when the mesh number of the filter screen is too low, so that the taste of the substitutional tea is influenced; drying with 50 deg.C hot air during filtering; the air outlet device can be arranged to be an air outlet pipe of the butt joint dust remover, and the powder removing effect in the drying process is further improved.

S5, sterilization: introducing the dried tea raw material into a closed loop consisting of a fluidized bed, an ozone generator, an ozone detector and a gas storage bin, and sterilizing the tea raw material in a circulating flow manner in an ozone airflow in a closed environment;

the method specifically comprises the following steps:

s51, sequentially connecting the fluidized bed, the ozone generator and the gas storage bin, connecting the fluidized bed with a material outlet of the filtering device, setting a material and gas circulation channel as a closed loop, and arranging an ozone detector in the closed loop;

s52, starting an ozone generator, and controlling the concentration of ozone gas in the closed loop to be between 15 ppm and 25ppm and keeping the ozone gas stable; the ozone gas with too high concentration has higher requirement on the power of the ozone generator, and consumes electric energy; the ozone gas concentration is too low to make the ozone fully contact with the substitutional tea particles, thereby affecting the disinfection effect.

And S53, introducing the dried material into a fluidized bed in a closed loop, and sterilizing the substituted tea raw material in an ozone airflow in a closed environment in a circulating flow manner.

In step S5, the concentration of ozone gas in the closed environment can be controlled between 15 ppm and 25ppm, the material ratio of the ozone to the substitute tea raw material is 1.2g to 1.6g to 100kg, and the rotating speed of the fluidized bed is 70 r/min to 90 r/min.

Step S5, the time for carrying out the disinfection is controlled to be 25-40min, and the disinfection effect can be achieved when the ozone quantity generated by the machine reaches a certain concentration due to the one-time instant disinfection principle of the ozone; if the time is too short, the ozone is not fully contacted with the raw material particles, and the requirement of the national standard on the disinfection effect is difficult to meet; if the disinfection time is too long, the half-life period of the ozone is short, so that the decline of the ozone and the growth of bacteria form dynamic balance, and the disinfection efficiency is further reduced if the better disinfection effect is not achieved.

S6, subpackaging: packaging the sterilized tea substitute raw materials into bags. Before subpackaging, the bacterial colony numbers and the total bacterial colony numbers of staphylococcus aureus, shigella, salmonella and escherichia coli of the sterilized substituted tea raw materials can be measured.

Throughout the sterilization process, one or more of pressure, flow rate, pH, ozone generation rate in the closed circuit are controlled by closed loop computer control to maintain predetermined sterilization conditions stable.

The following is illustrated in detail by examples:

example 1

S1, cleaning: selecting fresh poria cocos as a substitute tea raw material, removing required rhizome, bud leaf, flower, bud or fruit according to the type of the substitute tea raw material, and cleaning with clear water for later use;

s2, crushing: pulverizing various S1 processed substitutional tea raw materials into powder with average diameter of 250 μm;

s3, batching: mixing the crushed various substitutional tea raw materials;

s4, removing powder and drying: passing the raw materials prepared in the step S3 through a 40-mesh filter screen, drying by using hot air at 50 ℃ in the filtering process, and using an air outlet pipe air outlet device of a butt joint dust remover;

s5, sterilization: s51, sequentially connecting the fluidized bed, the ozone generator and the gas storage bin, connecting the fluidized bed with a material outlet of the filtering device, setting a material and gas circulation channel as a closed loop, and arranging an ozone detector in the closed loop;

s52, starting an ozone generator, controlling the concentration of ozone gas in the closed loop to be between 15 ppm and 25ppm and keeping the ozone gas stable, controlling the material ratio of ozone introduction to the substitute tea raw material to be 1.2g:100kg, and controlling the rotating speed of the fluidized bed to be 70/min;

and S53, introducing the dried material into a fluidized bed in a closed loop, and circularly flowing the substituted tea raw material in ozone airflow in a closed environment for disinfection for 25 min.

S6, subpackaging: packaging the sterilized tea substitute raw materials into bags. Before being dispensed, the sterilized substituted tea raw material can be measured for the number of colonies of staphylococcus aureus, shigella, salmonella, and escherichia coli and the total number of colonies, as described in experimental example 2 below.

Example 2

S1, cleaning: selecting fresh arillus longan as a substitute tea raw material, removing required rhizome, bud leaf, flower, bud or fruit according to the type of the substitute tea raw material, and cleaning with clear water for later use;

s2, crushing: pulverizing various S1 processed substitutional tea raw materials into powder with average diameter of 350 μm;

s3, batching: mixing the crushed various substitutional tea raw materials;

s4, removing powder and drying: passing the raw materials prepared in the step S3 through a 60-mesh filter screen, drying by using hot air at 50 ℃ in the filtering process, and using an air outlet pipe air outlet device of a butt joint dust remover;

s5, sterilization: s51, sequentially connecting the fluidized bed, the ozone generator and the gas storage bin, connecting the fluidized bed with a material outlet of the filtering device, setting a material and gas circulation channel as a closed loop, and arranging an ozone detector in the closed loop;

s52, starting an ozone generator, controlling the concentration of ozone gas in the closed loop to be between 15 ppm and 25ppm and keeping the ozone gas stable, controlling the material ratio of ozone introduction to the substitute tea raw material to be 1.6g:100kg, and controlling the rotating speed of the fluidized bed to be 90/min;

and S53, introducing the dried material into a fluidized bed in a closed loop, and sterilizing the substituted tea raw material in the ozone airflow in the closed environment for 40min in a circulating flow manner.

S6, subpackaging: packaging the sterilized tea substitute raw materials into bags. Before being dispensed, the sterilized substituted tea raw material can be measured for the number of colonies of staphylococcus aureus, shigella, salmonella, and escherichia coli and the total number of colonies, as described in experimental example 2 below.

Example 3

S1, cleaning: selecting a fresh mixture of fructus sophorae and fructus lycii as a substitute tea raw material, removing required rhizome, bud leaves, flowers, buds or parts except fruits according to the type of the substitute tea raw material, and cleaning with clear water for later use;

s2, crushing: pulverizing various S1 processed substitutional tea raw materials into powder with average diameter of 300 μm;

s3, batching: mixing the crushed various substitutional tea raw materials;

s4, removing powder and drying: enabling the raw materials prepared in the step S3 to pass through a 50-mesh filter screen, drying by using hot air at 50 ℃ in the filtering process, and using an air outlet pipe air outlet device of a butt joint dust remover;

s5, sterilization: s51, sequentially connecting the fluidized bed, the ozone generator and the gas storage bin, connecting the fluidized bed with a material outlet of the filtering device, setting a material and gas circulation channel as a closed loop, and arranging an ozone detector in the closed loop;

s52, starting an ozone generator, controlling the concentration of ozone gas in the closed loop to be between 15 ppm and 25ppm and keeping the ozone gas stable, controlling the material ratio of ozone introduction to the substitute tea raw material to be 1.5g:100kg, and controlling the rotating speed of the fluidized bed to be 80/min;

and S53, introducing the dried material into a fluidized bed in a closed loop, and sterilizing the substituted tea raw material in the ozone airflow in the closed environment for 30min in a circulating flow manner.

S6, subpackaging: packaging the sterilized tea substitute raw materials into bags. Before being dispensed, the sterilized substituted tea raw material can be measured for the number of colonies of staphylococcus aureus, shigella, salmonella, and escherichia coli and the total number of colonies, as described in experimental example 2 below.

Experimental example 1 sensory index test of tea substitute product

First, preparation of experiment

1. The experimental principle is as follows: the appearance, soup color, aroma and taste characteristics of the product are evaluated through the normal visual, olfactory, gustatory and tactile senses of the inspectors

2. Grouping products: the substitute tea product processed by the method of the embodiment 3 is an invention group; the substitute tea product prepared by the prior art is used as a comparison group for carrying out sensory index inspection, and the substitute tea product of the comparison group in the experimental example is the sophora fruit and wolfberry fruit mixed substitute tea prepared by the method such as CN 90102526.7.

Second, Experimental methods

1. Selecting a room floor with bright and soft light and natural light or a standard synthetic light source; the floor and the wall are not reflective, and the color of the inspection room is light. The room temperature is kept at about 25 ℃, the room is quiet and free of noise interference, and the table top is not reflective.

2. The white porcelain evaluation tea cup is selected, has consistent color and luster, 65mm height, 66mm outer diameter, 62mm inner diameter and 150mL capacity, is provided with a cover and a small hole, and is provided with a zigzag gap on the upper edge of the cup handle corresponding to the cup opening.

3. The water for evaluating tea is selected from water meeting the national drinking water regulations, is clean and odorless, has a pH value of 6.5-8.5, and is preferably purified water.

4. And (4) checking the content: the method is characterized by comprising the following steps of (1) carrying out inspection according to four sensory indexes of appearance, liquor color, aroma and taste: whether the tea has the characteristics and inherent color of the product is checked by appearance, whether the same product is uniform in size, has deterioration and has obvious pest and disease damage traces; the soup color inspection is mainly used for observing the color, the depth, the light and shade, the turbidity and the like of the soup; the aroma test is mainly used for evaluating the types, purity, height, durability, freshness and the like of the aroma; the tea soup concentration, purity and the like are evaluated mainly by taste inspection.

Third, checking step

1. And (3) appearance inspection: using a sample splitter to separate 100-250g of samples from the samples to be detected, placing the samples in a sample tray, operating the sample tray for several times to layer the samples according to the thickness and the size, and evaluating the color, the cleanness, the uniformity, the presence of deterioration and the presence of mildew; and selecting 30 persons to evaluate the appearances of the two groups of samples, grading according to a full score of 5, and counting the evaluation results.

2. Inspecting liquor color, aroma and taste: 3.0g of the sample is taken according to the product characteristics, placed in a tea evaluation cup, filled with boiling water and covered. Soaking leaves and flowers for 4 min; soaking fruits, rhizomes and mixed materials for 5min, draining the tea soup into the evaluation tea bowl, and observing the color, aroma and taste of the tea soup in sequence; selecting 30 people to evaluate the soup colors of the two groups of tea soup, grading according to a full score of 5, and counting the evaluation results.

Fourth, experimental results

1. Statistical results

Table 1: appearance test results

The appearance of the products of the invention group and the comparison group has the inherent color of the product, and the products have no deterioration, mildew or impurities; the invention group and the comparison group have inherent fragrance of each product and have no peculiar smell; both the inventive group and the comparative group had intrinsic flavors of the respective products without off-flavor.

The inventive tea soup was clear and bright in color, and the comparative tea soup was turbid in color and had a small amount of fine powder.

2. Conclusion of the experiment

The color and the color clarification degree of the tea soup obtained by the processing method of the substituted tea raw material are obviously stronger than those of a contrast group tea soup, the appearance of the invention group is similar to that of the contrast group in a test evaluation result, and the evaluation of the invention group soup color by an evaluation person is obviously higher than that of the contrast group, so that the processing method of the substituted tea raw material can solve the problem that fine powder of the crushed substituted tea raw material mostly influences the color of the tea soup, and obtain a substituted tea soup product with clear and bright tea color.

Experimental example 2 Disinfection Effect test of substitute tea product

First, preparation of experiment

1. Grouping products: the substitute tea product prepared by applying the method of the embodiment 3 of the invention is an invention group; the substitute tea product made by the prior art (wherein the sterilization step adopts irradiation sterilization) is taken as the prior art group; selecting the tea substitute raw material before processing as a control group, sampling 25g respectively, and carrying out the tests on the colony number and the total colony number of the staphylococcus aureus.

2. An experimental instrument: a constant temperature incubator, a balance, a homogenizer, an oscillator, a sterile suction tube, a sterile conical flask, a sterile culture dish and an injector.

3. In the experiment, the number of the staphylococcus aureus colonies and the total number of the staphylococcus aureus colonies in the product are tested as an example to verify the disinfection effect of the substitute tea product, in the actual operation, the disinfection effects of shigella, salmonella and escherichia coli are respectively tested and the content of the shigella, salmonella and escherichia coli is measured according to corresponding detection methods in GB4789.5-2012, GB4789.4-2010 and GB/T4789.3-2008, relevant experimental methods are respectively formulated according to records in the experiment, and relevant experimental parameters are adjusted by technicians in the field according to technical common knowledge in the field.

Second, Experimental methods

1. Staphylococcus aureus assay

Respectively weighing 25g of samples of the invention group, the prior art group and the control group into a sterile homogenizing cup filled with 225mL of 7.5 percent sodium chloride broth, and homogenizing at 8000r/min for 1.5 min; culturing the sample homogeneous solution for 24 hours at the temperature of 36 +/-1 ℃; the cultures are respectively streaked and inoculated to a Baird-Parker plate, and are respectively cultured for 24h under the environment of 36 +/-1 ℃.

2. Staphylococcus aureus Baird-Parker plate count

Respectively weighing 25g of the samples of the invention group, the prior art group and the control group into a sterile homogenizing cup containing 225mL of phosphate buffer solution, and homogenizing at 8000r/min for 1.5min to obtain 1:10 sample homogenizing solution; aspirate 1: slowly injecting 1mL of 10 sample homogenizing solution into a sterile test tube containing 9mL of diluent along the tube wall, and shaking the test tube to mix the sample homogenizing solution uniformly to prepare 1:100 sample homogenizing solution; a10-fold serial dilution sample homogenizing solution is prepared according to the steps, and a sterile suction pipe with the volume of 1mL is used in turn when the dilution is gradually increased.

Sample aliquots were selected at dilutions of 1:10, 1:100 and 1:1000, and at 10-fold incremental dilutions, 1mL of sample aliquot was aspirated at each dilution and added to three Baird-Parker plates at 0.3mL, 0.4mL inoculum sizes, followed by coating the entire plate with a sterile L-bar.

After coating, the plate is kept stand for 10min, the plate is turned over after the sample homogenizing liquid is absorbed, and the plate is placed upside down in an incubator to be cultured for 48h under the environment of 36 +/-1 ℃.

3. Plasma coagulase assay

1 suspected colony on the Baird-Parker plate is picked up and inoculated to 5mLBHI and nutrient agar small inclined plane to be cultured for 24h under the environment of 36 +/-1 ℃. Taking 0.5mL of freshly prepared rabbit plasma, placing the rabbit plasma into a small test tube, adding 0.2-0.3 mL of BHI culture, shaking the rabbit plasma uniformly, placing the rabbit plasma in an incubator at 36 +/-1 ℃, observing the rabbit plasma once every half hour for 6 hours, and judging the rabbit plasma to be positive if the coagulation phenomenon occurs.

Third, experimental data and processing

1. Observing the colony distribution and appearance state on a Baird-Parker flat plate by visual observation and a dyeing mirror;

2. plates with typical S.aureus colonies were selected and the number of all colonies on the same dilution 3 plates was summed to a plate between 20CFU and 200CFU and the typical colony count was counted.

3. Calculating the total number of the counted typical colonies with each concentration according to a formula T ═ AB/Cd, wherein T is the number of staphylococcus aureus colonies in the sample; a is the total number of typical colonies at a certain dilution; b is the number of colonies positive for plasma coagulase at a certain dilution; c is the colony number of a certain dilution for the plasma coagulase test; d is the dilution factor, i.e. the first dilution.

4. Statistical results were analyzed for variance using SPSS16.0 software and are expressed as mean ± standard deviation. Are constructed in P < The 0.05 difference was statistically significant.

Fourth, experimental results

Visual observation shows that more gray to black colonies exist on the Baird-Parker plate of the control group, the edges of the colonies are light, and turbid zones exist around the colonies; under the stainboscopy, three groups of samples all find grape-shaped arranged bacteria, have no spores and no capsules, the diameter is between 0.5 and 1 mu m, and the bacteria amount detected by the prior art group and the invention group is far less than that of the control group.

Experimental results show that the disinfection and sterilization effect of the processing method of the substituted tea raw material can kill about 99% of staphylococcus aureus in the substituted tea raw material, and the disinfection and sterilization effect is not inferior to that of the existing irradiation sterilization technology.

The present invention is not limited to the above-described embodiments, and it will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the principle of the present invention, and such modifications and improvements are also considered to be within the scope of the present invention. Those not described in detail in this specification are within the skill of the art.