阅读说明：本技术 厨余垃圾、秸秆植残、粪便资源化转化、除臭及生物菌肥生产技术 (Kitchen garbage, straw plant residue, excrement resource conversion, deodorization and biological bacterial fertilizer production technology ) 是由 王昱蓉 季静 王罡 于 2021-06-16 设计创作，主要内容包括：厨余垃圾、秸秆植残、粪便资源化转化、除臭及生物菌肥生产技术。本发明涉及新分类的具有强降解纤维素和植物促生功能菌特异菌JiTF01,及其与其他一种或者多种菌按一定比例,在天福CCQC325培养基中发酵培养中,在1-6级生产菌剂技术及工艺下生产形成菌剂并包装成菌剂产品；进一步对厨余垃圾、植残、粪便等有机废弃物资源化转化、除臭,复合菌剂与配套小型终端设备共同作用,通过快速降解植物残体、秸秆、厨余垃圾资源化转化成有机固废资源化转化菌肥的技术,和降解粪便和快速除臭及资源化转化形成有机固废资源化转化菌肥技术,将有机废弃物转化成含有小分子的有机固废资源化转化菌肥和生物菌剂。可在农业、种植业、养殖业及环境生态治理中应用。(Kitchen garbage, straw plant residue, excrement resource conversion, deodorization and biological bacterial manure production technology. The invention relates to a new classified specific bacterium JiTF01 with strong degradation cellulose and plant growth promoting function, and a bacterium agent which is produced and formed under the bacterium agent production technology and technology of 1-6 level and packaged into a bacterium agent product in a fermentation culture of Tianfu CCQC325 culture medium with one or more other bacteria according to a certain proportion; further performing resource conversion and deodorization on organic wastes such as kitchen wastes, plant residues and excrement, and converting the organic wastes into small-molecule organic solid waste resource conversion bacterial manure and biological agents by the combined action of the compound microbial inoculum and matched small-size terminal equipment through a technology for quickly degrading plant residues, straws and the kitchen wastes and converting the plant residues, the straws and the kitchen wastes into the organic solid waste resource conversion bacterial manure and a technology for degrading the excrement, quickly deodorizing and converting the wastes into the organic solid waste resource conversion bacterial manure. Can be applied to agriculture, planting industry, breeding industry and environmental ecological management.)

1. A strain of Penicillium sp.JiTF01 (JiTF01 for short) with strong cellulose degradation and plant growth promotion functions is separated, and the strain has strong salt and acid resistance (pH 2), strong cellulase activity, nitrogen fixation and phosphorus dissolution, forms a composite microbial inoculum with other strains, can quickly degrade plant residues, straws, kitchen garbage, organic wastes, excrement, organic floating materials discharged by light plants which are difficult to degrade and the like in matched small-sized equipment, and can be grown in plants. The organic waste degradation product contains more thalli, and forms a technology for recycling in agriculture, planting industry, breeding industry and environmental ecological management.

2. The method of claim 1, wherein the new strain (JiTF01) and the auxiliary strain are used to form a rapid degradation microbial inoculum and the proportion thereof.

3. The optimal raw material formula of the microbial inoculum production medium Tianfu CCQC 325.

4. The grade 1-6 microbial inoculum production technology and the packaging of complex microbial inoculum products according to claim 1.

5. The complex microbial inoculant of claim 1 matched with small-sized terminal equipment.

6. The technology for resource conversion of organic solid wastes into bacterial manure by fast degrading plant residues and kitchen wastes according to claim 1.

7. The technology for degrading excrement, quickly deodorizing and converting resources into organic solid waste and resource converting bacterial manure according to claim 1.

8. The organic waste is converted into micromolecular biological degradation products, biological bacteria agents and biological organic fertilizers according to the resource of the organic waste of claim 1, and the micromolecular biological degradation products, the biological bacteria agents and the biological organic fertilizers are applied to soil improvement, agriculture, planting industry, breeding industry and environmental ecological management.

Technical Field

The invention relates to a biological agent and biological bacterial fertilizer production technology for resource conversion and deodorization of kitchen garbage, straw plant residues and excrement by using specific microbial agents, culture media and matched small-sized equipment, belongs to the field of applied biotechnology, converts organic wastes into micromolecular organic solid waste resource conversion bacterial fertilizers and organic solid waste resource conversion biological microbial agents, and can be applied to agriculture, planting industry, breeding industry and environmental ecological management.

Background

The key of the breakthrough of the urban kitchen garbage recycling technology is the innovation of environment-friendly new degrading strains and matched small-sized equipment so as to form efficient resource recycling and cyclic utilization. 2 degradation type saline-alkali resistant growth-promoting bacteria are separated, can degrade plant residues, has salt concentration as high as 19 percent, fixes nitrogen, dissolves phosphorus excrement and the like, and can grow in the beginning of plants. Research shows that the composite bacterial strain is used with other bacteria, has the capability of quickly degrading kitchen garbage (8 hours) and quickly deodorizing (1.5 hours), and biologically degrades and converts the kitchen garbage into small molecules which can be planted and utilized. The method is bound to recycle kitchen garbage resource conversion and conversion products in planting and environmental ecological management.

Compared with the existing biological bacterial manure conversion technology of 15 days to 6 months, the technology can convert the plant residues and the kitchen garbage into the biological bacterial manure and the microbial inoculum within 3 to 8 hours, and has great advantages and uniqueness.

Further, the research on the specific microbial inoculum and matched small-sized equipment by the technology can quickly convert kitchen garbage into mixed microbial inoculum of nutrient micromolecules and beneficial bacteria and a biological organic fertilizer, can be safely and resourcefully applied to agriculture, planting industry and breeding industry, and can also be used for environmental management. The method has wide industrialization prospect and large market demand, and the industry can drive agriculture, planting industry, breeding industry and environmental ecological management.

Disclosure of Invention

The key of the breakthrough of the urban kitchen garbage recycling technology is the innovation of environment-friendly new degrading strains and matched small-sized equipment so as to form efficient resource recycling and cyclic utilization.

The invention content is as follows:

1. a strain of Penicillium sp.JiTF01 (JiTF01 for short) with strong cellulose degradation and plant growth promotion functions is separated, and the strain has strong salt and acid resistance (pH 2), strong cellulase activity, nitrogen fixation and phosphorus dissolution, forms a composite microbial inoculum with other strains, can quickly degrade plant residues, kitchen garbage, organic wastes, excrement, organic floating materials discharged by light plants which are difficult to degrade and the like in matched small-sized equipment, and can initially grow plants. The organic waste degradation product contains more thalli, and forms a technology for recycling in agriculture, planting industry, breeding industry and environmental ecological management.

2. The new strain (JiTF01) and the auxiliary strain are used to form a rapid degradation microbial inoculum and the proportion thereof.

3. Optimal raw material formula of microbial inoculum production culture medium Tianfu CCQC325 culture medium

4. 1-6 grade bacteria producing technology and composite bacteria product package

5. Small terminal equipment matched with complex microbial inoculum

6. A technology for quickly degrading plant residues and converting kitchen garbage into organic solid waste into a bacterial manure.

7. Degrading excrement, quickly deodorizing and converting into resources to form the organic solid waste and resource converting bacterial manure technology.

8. Organic waste is converted into micromolecular biodegradable substances, biological bacteria agents and biological organic fertilizers in a recycling way, and the micromolecular biodegradable substances, the biological bacteria agents and the biological organic fertilizers are applied to soil improvement, agriculture, planting industry, breeding industry and environmental ecological management.

The technical description is as follows:

1. a strain of Penicillium sp.JiTF01 (JiTF01 for short) with strong cellulose degradation and plant growth promotion functions is separated, and the strain has strong salt and acid resistance (pH 2), strong cellulase activity, nitrogen fixation and phosphorus dissolution, forms a composite microbial inoculum with other strains, can quickly degrade plant residues, kitchen garbage, straws, organic wastes, excrement and the like in matched small-sized equipment, and can grow in plants. The organic waste degradation product contains more thalli, and forms a technology for recycling in agriculture, planting industry, breeding industry and environmental ecological management.

Penicillium sp. JiTF01 (JiTF01 for short) has the functions of degrading cellulose and plant residue, resisting salt and acid (pH 2), fixing nitrogen and phosphorus, and promoting plant growth. The strain is used in combination with other strains, has the capability of rapidly degrading kitchen garbage (8 hours) and rapidly deodorizing (1.5 hours), and biologically degrades and converts the kitchen garbage into small molecules which can be planted and utilized. Can quickly degrade plant residues, kitchen garbage, organic wastes, excrement and the like in matched small equipment, and can grow in the beginning. The organic waste degradation product contains more thalli, and forms a technology for recycling in agriculture, planting industry, breeding industry and environmental ecological management.

2. The new strain (JiTF01) and the auxiliary strain are used to form a rapid degradation microbial inoculum and the proportion thereof.

The JiTF01 is mixed with one or more of the following microbial inoculum to form a compound microbial inoculum, wherein the compound microbial inoculum is different in application and function. The microbial inoculum mixed with the JiTF01 comprises the following components:

(1) bacillus natto (Bacillus natto): TRIIM 1.01025 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(2) Bacillus licheniformis (Bacillus licheniformis): TRIIM 1.00400 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(3) Bacillus amyloliquefaciens (Bacillus amyloliquefaciens): TRIIM 1.00303 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(4) Bacillus subtilis (Bacillus subtilis): TRIIM 1.01001 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(5) Streptococcus thermophilus (Streptococcus thermophilus) CICC 6038 is from China center for Industrial culture Collection of microorganisms (CICC) and can be obtained by the public from the China center for culture Collection of Industrial microorganisms.

(6) Lactobacillus plantarum CICC 21810 originates from the China center for Industrial microbial cultures Collection (CICC) and is available to the public from the China center for Industrial microbial cultures Collection.

(7) Lactobacillus rhamnosus (Lactobacillus rhamnosus) CICC 20257 is originated from the China center for Industrial microbial cultures Collection (CICC) and can be obtained by the public from the China center for Industrial microbial cultures Collection.

(8) Lactobacillus casei (Lactobacillus casei) CICC 23185 is from the China center for Industrial microbial culture Collection (CICC) and is available to the public from the China center for Industrial microbial culture Collection.

(9) Lactobacillus paracasei (Lactobacillus casei) CICC 20252 is from the China center for Industrial microbial culture Collection (CICC) and is available to the public from the China center for Industrial microbial culture Collection.

(10) Lactobacillus acidophilus (Lactobacillus acidophilus) CICC 20244 is originated from the China center for the culture Collection of Industrial microorganisms (CICC), and the strain is available to the public from the China center for the culture Collection of Industrial microorganisms.

The optimal ratio of the microbial inoculum for the softer kitchen waste which can be used as feed after being recycled is as follows:

(1) the ratio of JiTF01 is 10%.

(2) 10% of glutamic acid bacterium halophila.

(3) 5 percent of natto bacillus.

(4) 10% of bacillus licheniformis.

(5) 10% of bacillus amyloliquefaciens.

(6) 5 percent of bacillus subtilis.

(7) 10% of streptococcus thermophilus.

(8) 10% of lactobacillus plantarum.

(9) 10% of lactobacillus rhamnosus.

(10) 10% of lactobacillus casei.

(11) 10% of lactobacillus paracasei.

The formula is also used for resourceful treatment of excrement and urine and the like.

The optimal common ratio of the microbial inoculum for degrading plant residues and hard kitchen garbage is as follows:

(1) the ratio of JiTF01 is 15%.

(2) 10% of glutamic acid bacterium halophila.

(3) 5 percent of natto bacillus.

(4) 10% of bacillus licheniformis.

(5) And 15% of bacillus amyloliquefaciens.

(6) 5 percent of bacillus subtilis.

(7) 10% of streptococcus thermophilus.

(8) And 5% of lactobacillus plantarum.

(9) 10% of lactobacillus rhamnosus.

(10) 5% of lactobacillus casei.

(11) Lactobacillus paracasei 10%

3. Tianfu CCQC325 culture medium for microbial inoculum production culture medium

The microbial inoculum production culture medium is also called Tianfu CCQC325 culture medium, which can be 0.5-20% according to different purposes, and the vitamins, the carotenoid and the grape seed extract are lower, and the available proportion is as follows:

(1) corn starch 0-200g

(2) Hydrolyzed corn protein extract 0-200g

(3) Soybean polypeptide 0-200g

(4) 0-200g of soybean milk powder

(5) 0-200g of milk powder

(6) 0-200g of brown sugar

(7) 0-200g Tianfu nutritious rice flour

(8)DVC 0-50g

(9) Edible salt 0-50g

(10) 0-50g of additives such as various vitamins, nutritional additives and trace elements

(11) Carotenoid and grape extract 0-50g (without nutrition rice or semen Maydis)

(12) 5L of water

Carotenoid and grape extract (without nutrition of rice and corn).

The optimal raw material formula is as follows:

the culture medium liquid state fermentation culture medium raw materials can be flexibly adjusted according to the situation, the proportion of partial raw materials is increased, or partial raw materials are reduced or even not used, and the optimal raw material proportion is researched as follows:

(1) corn starch 50g

(2) Hydrolyzed corn protein extract 50ml

(3) Soybean peptide 50g

(4) Soybean milk powder 50g

(5) Milk powder 50g

(6) Brown sugar 200g

(7) Tianfu nutritious rice flour 8g

(8)DVC 5g

(9) Edible salt 5g

(10) 1g of additives such as various vitamins, nutritional additives and trace elements

(11) Carotenoid and flavonoid (not using nutritional rice and corn) 0.5g

(12) 5L of water.

4. 1-6 grade bacteria producing technology and composite bacteria product package

(1) Grade 1-3 fermentation vessel and culture medium, sterilizing with autoclave for 15-25 min, preferably 20 min.

(2) After the 4-6 stage fermentation medium was dissolved in water, it was boiled for 12 minutes.

(3) And (4) sterilizing the large container for 30 minutes to 2 hours by using ultraviolet and ozone.

(4) Pouring the sterilized culture medium into a fermentation container, fermenting at 28-39 deg.C and optimal temperature of 32-33 deg.C for 25 hr to 1 week, and optimal time of 48-72 hr.

(5) Primary fermentation, wherein single bacteria are fermented respectively to form pure strains.

(6) And (4) performing secondary fermentation, wherein 1-2 liters of secondary single strains are formed by fermentation mainly by using the expanded fermentation single strains.

(7) And (3) third-stage fermentation, namely mixing the strains which are not mutually inhibited and are single strains of the second-stage expanded fermentation with each other, fermenting to form 2-5L of third-stage strains, and dividing the strains into 5-6 groups of bacteria for fermentation.

(8) Four-stage fermentation, namely performing 5-20L fermentation on the divided 5-6 types of fermentation flora in a simple fermentation tank or a common fermentation tank.

(9) And (3) performing five-stage fermentation, namely mixing the floras obtained by the four-stage expanded fermentation, and fermenting to form 20-50 liters.

(10) Six-stage expansion and production fermentation, wherein five-stage fermentation seed bacteria are added according to the proportion of 10-20 percent, and 100-500L or even several tons of expansion or production fermentation are carried out. Cleaning a fermentation tank before fermentation, and cleaning with tap water once; the surfactant is cleaned once; and (5) washing for 3 times by using tap water, and sterilizing a pipeline and a fermentation tank. Fermenting at an optimal temperature of 32-33 ℃ for 25-72 hours, and optionally prolonging the fermentation time to one week.

(11) The formed microbial inoculum is distributed and packaged into a 500-sand 1000ml bottle, and can be used commercially or only.

5. Small terminal equipment matched with complex microbial inoculum

Schematic diagram of small kitchen garbage and excrement straw resource bacterial manure fermentation equipment:

small terminal equipment matched with complex microbial inoculum

A schematic diagram of a small kitchen garbage and excrement straw resource bacterial manure fermentation device is shown in figure 1.

Equipment capacity: 0.2-2m3, daily throughput: 50-200Kg, rated voltage: 220-380V, rated power: 5KW, treatment temperature: 30-50 ℃.

Wherein the stirrer parts are shown in figure 2.

The main part of the device is a stirrer, and other crushers such as kitchen garbage and straw crushing feeding positions can be purchased, assembled or carried out in parts; the dung inlet pump can be assembled and installed if dung and urine are required to be recycled according to the situation; heating wires, solar generators, temperature numerical control display screens and the like can be purchased and installed in the market. The device has the characteristics of portability, convenient assembly and convenient use.

The main part of the device is a stirrer, and other crushers such as kitchen garbage and straw crushing feeding positions can be purchased, assembled or carried out in parts; the dung inlet pump can be assembled and installed if dung and urine are required to be recycled according to the situation; heating wires, solar generators, temperature numerical control display screens and the like can be purchased and installed in the market. The device has the characteristics of portability, convenient assembly and convenient use.

6. Technology for resource conversion of straw, plant residues and kitchen garbage into organic solid waste resource conversion bacterial manure

(1) Crushing plant residues, straws and kitchen garbage by using the equipment in the step 5, putting the crushed plant residues, straws and kitchen garbage into the equipment, cooling the crushed plant residues, straws and kitchen garbage to 26-38 degrees (optimally 33 degrees) naturally for 5-10 minutes at 60-100 degrees;

(2) adding cellulase and hemicellulase, stirring the bacteria, plant residue and kitchen garbage crushed material with a stirrer for 1-5 hr, stirring at 26-37 deg.C (preferably 33 deg.C) and 30-200 rpm (or manual stirring).

(3) Adding the above 4 microbial inoculum into equipment, stirring with a stirrer, stirring uniformly for 2-8 hr, stirring at 26-37 deg.C (preferably 33 deg.C), and stirring at 30-200 rpm (or manual stirring).

(4) And (5) pouring out the fermented plant residues and the kitchen waste microbial inoculum mixture from a discharge hole of the equipment in the step 5.

(5) The resource conversion of the plant residues and the kitchen garbage can be controlled within 3-8 hours, and the after-ripening can be carried out after the conversion microbial inoculum is bagged to form the organic solid waste resource conversion bacterial fertilizer.

7. Degrading excrement, quickly deodorizing and converting into resources to form the organic solid waste and resource converting bacterial manure technology.

(1) Sucking feces into the apparatus of the above 5 at 60-100 deg.C for 5-10 min, and naturally cooling to 26-38 deg.C (optimally 33 deg.);

(2) adding cellulase and hemicellulase, stirring with a stirrer at 26-37 deg.C (preferably 33 deg.C) and 30-200 rpm (or manual stirring).

(3) Adding the microbial inoculum obtained in the step 4 into equipment, stirring uniformly by a stirrer, and stirring at 26-37 degrees (optimally 33 degrees) after 8 hours at 30-200 revolutions (manual stirring is also available).

(4) And (5) pouring out the fermentation inoculum mixture from a discharge hole of the equipment in the step 5.

(5) And after the transformation microbial inoculum is bagged, after-ripening is carried out, so as to form the organic solid waste resource transformation bacterial fertilizer.

8. Organic waste is converted into micromolecular biodegradable substances, biological bacteria agents and biological organic fertilizers in a recycling way, and the micromolecular biodegradable substances, the biological bacteria agents and the biological organic fertilizers are applied to soil improvement, agriculture, planting industry, breeding industry and environmental ecological management.

Description of the drawings:

FIG. 1 is a schematic view of a small kitchen garbage and excrement straw resource bacterial manure fermentation device.

Equipment capacity: 0.2-2m3, daily throughput: 50-200Kg, rated voltage: 220-380V, rated power: 5KW, treatment temperature: 30-50 ℃.

FIG. 2 is a diagram of the components of the agitator.

FIG. 3 shows the effect of pH, inoculum size and incubation time on cellulase activity of strain JiTF 01. a pH, b inoculum size and c incubation time.

FIG. 4 is a graph showing the effect of diluted fermentation broth on rice seedlings. Effect of different dilutions of fermentation broth and blank control on rice seedling growth. a is a statistical curve graph; b, photographs of rice seedlings treated by fermentation liquors with different dilutions.

FIG. 5 shows the effect of diluted fermentation broth on the salt stress germination rate of rice seedlings, and the effect of different dilutions of fermentation broth and blank control on the salt stress growth of rice seedlings at 100mM and 200 mM. a is a statistical curve graph; b is a photograph of rice seed germination treated by fermentation liquor with different dilutions.

Detailed Description

Example 1

The key of the breakthrough of the urban kitchen garbage recycling technology is the innovation of environment-friendly new degrading strains and matched small-sized equipment so as to form efficient resource recycling and cyclic utilization.

The technology discloses a strain of bacteria with strong cellulose degradation and plant growth promoting functions, namely Penicillium sp.JiTF01 (JiTF01 for short), which has the advantages of salt and acid resistance (pH 2), strong cellulase activity, nitrogen fixation and phosphorus dissolution, forms a composite microbial inoculum with other strains, can quickly degrade plant residues, kitchen garbage, organic wastes, excrement and the like in matched small-sized equipment, and can grow in the beginning of plants. The organic waste degradation product contains more thalli, and forms a technology for recycling in agriculture, planting industry, breeding industry and environmental ecological management.

Experiments were conducted to investigate the effect of the pH, inoculum size and incubation time of JiTF01 on cellulase activity of strain JiTF01, as shown in FIG. 3, a pH, b inoculum size and c incubation time. The results show that the JiTF01 has acid resistance and pH value of 2, can normally grow, has high yield and high activity cellulase (see figure 3), and can promote plant growth (see figure 4) and plant germination (see figure 5).

Example 2

The new strain JiTF01 and auxiliary bacteria are used to produce a microbial inoculum which can degrade plant residues, kitchen garbage and excrement and can be directly recycled in agriculture, planting industry, breeding industry and environmental ecological management in a culture medium Tianfu CCQC325 culture medium.

The rapid degradation microbial inoculum and the proportion thereof are formed by utilizing the JiTF01 and the auxiliary bacteria. The JiTF01 can be mixed with one or more of the following bacterial agents to form a compound bacterial agent, wherein the compound bacterial agent has different purposes and functions. The microbial inoculum mixed with the JiTF01 comprises the following components:

(1) bacillus natto (Bacillus natto): TRIIM 1.01025 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(2) Bacillus licheniformis (Bacillus licheniformis): TRIIM 1.00400 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(3) Bacillus amyloliquefaciens (Bacillus amyloliquefaciens): TRIIM 1.00303 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(4) Bacillus subtilis (Bacillus subtilis): TRIIM 1.01001 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(5) Streptococcus thermophilus (Streptococcus thermophilus) CICC 6038 is from China center for Industrial culture Collection of microorganisms (CICC) and can be obtained by the public from the China center for culture Collection of Industrial microorganisms.

(6) Lactobacillus plantarum CICC 21810 originates from the China center for Industrial microbial cultures Collection (CICC) and is available to the public from the China center for Industrial microbial cultures Collection.

(7) Lactobacillus rhamnosus (Lactobacillus rhamnosus) CICC 20257 is originated from the China center for Industrial microbial cultures Collection (CICC) and can be obtained by the public from the China center for Industrial microbial cultures Collection.

(8) Lactobacillus casei (Lactobacillus casei) CICC 23185 is from the China center for Industrial microbial culture Collection (CICC) and is available to the public from the China center for Industrial microbial culture Collection.

(9) Lactobacillus paracasei (Lactobacillus casei) CICC 20252 is from the China center for Industrial microbial culture Collection (CICC) and is available to the public from the China center for Industrial microbial culture Collection.

(10) Lactobacillus acidophilus (Lactobacillus acidophilus) CICC 20244 is originated from the China center for the culture Collection of Industrial microorganisms (CICC), and the strain is available to the public from the China center for the culture Collection of Industrial microorganisms.

The optimal ratio of the microbial inoculum for the softer kitchen waste which can be used as feed after being recycled is as follows:

(1) the ratio of JiTF01 is 10%.

(2) 10% of glutamic acid bacterium halophila.

(3) 5 percent of natto bacillus.

(4) 10% of bacillus licheniformis.

(5) 10% of bacillus amyloliquefaciens.

(6) 5 percent of bacillus subtilis.

(7) 10% of streptococcus thermophilus.

(8) 10% of lactobacillus plantarum.

(9) 10% of lactobacillus rhamnosus.

(10) 10% of lactobacillus casei.

(11) 10% of lactobacillus paracasei.

The formulation is also useful for fecal management and the like.

The optimal common ratio of the microbial inoculum for degrading plant residues and hard kitchen garbage is as follows:

(1) the ratio of JiTF01 is 15%.

(2) 10% of glutamic acid bacterium halophila.

(3) 5 percent of natto bacillus.

(4) 10% of bacillus licheniformis.

(5) And 15% of bacillus amyloliquefaciens.

(6) 5 percent of bacillus subtilis.

(7) 10% of streptococcus thermophilus.

(8) And 5% of lactobacillus plantarum.

(9) 10% of lactobacillus rhamnosus.

(10) 5% of lactobacillus casei.

(11) Lactobacillus paracasei 10%

The microbial inoculum can be produced by Tianfu CCQC325 culture medium, and can be 0.5-20% according to different purposes, and the content of vitamins, carotenoid and grape seed extract is lower.

Example 3

The production technology and process of the microbial inoculum are divided into 1-6 grades.

(1) Grade 1-3 fermentation vessel and culture medium, sterilizing with autoclave for 15-25 min, preferably 20 min.

(2) After the 4-6 stage fermentation medium was dissolved in water, it was boiled for 12 minutes.

(3) And (4) sterilizing the large container for 30 minutes to 2 hours by using ultraviolet and ozone.

(4) Pouring the sterilized culture medium into a fermentation container, fermenting at 28-39 deg.C and optimal temperature of 32-33 deg.C for 25 hr to 1 week, and optimal time of 48-72 hr.

(5) Primary fermentation, wherein single bacteria are fermented respectively to form pure strains.

(6) And (4) performing secondary fermentation, wherein 1-2 liters of secondary single strains are formed by fermentation mainly by using the expanded fermentation single strains.

(7) And (3) third-stage fermentation, namely mixing the strains which are not mutually inhibited and are single strains of the second-stage expanded fermentation with each other, fermenting to form 2-5L of third-stage strains, and dividing the strains into 5-6 groups of bacteria for fermentation.

(8) Four-stage fermentation, namely performing 5-20L fermentation on the divided 5-6 types of fermentation flora in a simple fermentation tank or a common fermentation tank.

(9) And (3) performing five-stage fermentation, namely mixing the floras obtained by the four-stage expanded fermentation, and fermenting to form 20-50 liters.

(10) Six-stage expansion and production fermentation, wherein five-stage fermentation seed bacteria are added according to the proportion of 10-20 percent, and 100-500L or even several tons of expansion or production fermentation are carried out. Cleaning a fermentation tank before fermentation, and cleaning with tap water once; the surfactant is cleaned once; and (5) washing for 3 times by using tap water, and sterilizing a pipeline and a fermentation tank. Fermenting at an optimal temperature of 32-33 ℃ for 25-72 hours, and optionally prolonging the fermentation time to one week.

(11) The formed microbial inoculum is distributed and packaged into a 500-sand 1000ml bottle, and can be used commercially or only.

The technology can produce the microbial inoculum for degrading kitchen garbage, converting organic wastes into resources and deodorizing, and the microbial inoculum can also be directly applied to agriculture, planting industry, breeding industry and environmental ecological management.

Example 4 plant residue, kitchen waste, fecal degradation or so called resource transformation process is a microbial dominated biochemical reaction process.

And (5) utilizing a new strain degrading microbial inoculum to rapidly degrade plant residues, kitchen garbage, excrement and urine, and rapidly deodorize and convert the plant residues, the kitchen garbage, the excrement and the urine into resources in the matched small-sized terminal equipment.

The process of plant residue, kitchen waste, and feces degradation or resource transformation is a biochemical reaction process dominated by microorganisms.

Firstly, plant residues, kitchen garbage and excrement are crushed, heated and insufficiently mixed with cellulase and hemicellulose, and microbial inoculums are added to accelerate the decomposition of organic matters in microbial communities and promote compost maturity, wherein important factors are the reduction of the nutrient loss of the kitchen garbage including the emission of NH3, N2O and CO 2. Promoting the organic matter to be converted into humus and forming effective micromolecular degradation products.

Compared with a control group, in the compost mixed by adding the pig manure and the sawdust, the cumulative emission of NH3 of the addition group is reduced by 70.50%, the cumulative emission of N2O is reduced by 29.00%, the content of NO3 is improved by 50.00%, the content of total Kjeldahl nitrogen is improved by 59.00%, and the nitrogen retention is effectively realized. During the temperature rising and high temperature period of the compost, most carbon metabolic intermediate products such as saccharides are degraded by microorganisms to generate a large amount of CO 2; the metabolic pathway of the microorganism is adjusted, so that the generation of CO2 in the compost can be reduced, the organic matter is promoted to be converted into humus, and the carbon in the compost is effectively fixed.

Example 5 kitchen waste of vegetables, meat skin and bones is crushed and mixed with microbial inoculum for fermentation, and can be used in breeding industry.

The fresh kitchen garbage of vegetables, meat skin and bones is crushed and then mixed with the microbial inoculum of 3 for fermentation, so that the microbial inoculum can be used for the breeding industry.

The ratio of the microbial inoculum is as follows:

(1) the ratio of JiTF01 is 5%.

(2) 15% of natto bacillus.

(3) 10% of bacillus licheniformis.

(4) 10% of bacillus amyloliquefaciens.

(5) 15% of streptococcus thermophilus.

(6) 10% of lactobacillus plantarum.

(7) And 15% of lactobacillus rhamnosus.

(8) 10% of lactobacillus casei.

(9) Lactobacillus paracasei 10%

Preparing dog food additive, fish feed additive, pig, cattle and sheep feed, etc. If the project is developed, the novel microbial inoculum and matched small-sized equipment are researched, kitchen garbage is converted into mixed microbial inoculum of nutritional micromolecules and beneficial bacteria in a quick resource mode, and the mixed microbial inoculum can be safely applied to the breeding industry in a resource mode.

Example 6 resource utilization of organic waste to prepare small molecular biodegradable products, biological bacteria and organic solid waste resource utilization conversion bacterial manure for application in agriculture and planting industry.

Experiments show that compared with conventional fertilization, the application of the organic solid waste resource transformation bacterial fertilizer can promote the growth of all plants and increase the yield obviously, and the plants comprise grain crops: rice, corn, beans, potatoes, highland barley, broad beans, wheat, and the like; oil crops: oilseeds, cranberries, mustard, peanuts, flax, sunflowers, and the like; vegetable crops: radish, cabbage, celery, leek, garlic, shallot, carrot, snake melon, lotus flower, jerusalem artichoke, sword bean, coriander, asparagus lettuce, daylily, hot pepper, cucumber, tomato, coriander and the like; fruits: pear, green plum, apple, peach, apricot, walnut, plum, cherry, strawberry, Chinese pear, red date and other varieties; wild fruits: wild pear, wild apricot, wild peach, wild jujube, prunus cerasifera, sea buckthorn, etc.; feed crops: alfalfa, corn, green manure, milk vetch, and the like; medicinal crops: honeysuckle, ginseng, angelica, honeysuckle, mint, mugwort and the like. And the product avoids soil fertility reduction, crop quality deterioration and environmental pollution caused by long-term excessive use of chemical fertilizers. No pollution and is favorable to culturing soil fertility.

The organic matters generated by the metabolism of the bacterial strains in the organic solid waste resource transformation bacterial fertilizer are the basis of fertility, and are also the substrate for beneficial microorganism culture, and selected pesticide residues, food residues, environmental pollutants and the like are recycled and degraded, so that the environment is protected.

The application effect is reported by taking Chinese cabbage as an example. Culturing Chinese cabbage in a light incubator, applying the bacterial manure of the invention to one group, applying urea to a control group, applying the two groups of fertilizers conventionally, applying 60% of fertilizer amount to vegetable garden sandy soil in a test field before field planting in nutrient soil seedling culture treatment, uniformly distributing 3 parts of the remaining 40%, and spraying 10 times of water soaking solution on leaf surfaces in a green-returning period, a lotus-seat period and an initial heading period. The conventional fertilizer is applied for 3 times according to local habituation to the green returning period, the lotus throne period and the heading initial period, and the influence of the organic bacterial manure on the growth, yield and quality of the Chinese cabbage is observed.

Growth and yield observations:

the Chinese cabbage in the bacterial manure treatment area grows faster and has higher yield. The investigation result shows that the growth vigor is obviously better than that of urea by applying the bacterial manure.

TABLE 1 influence of organic solid waste resource transforming bacterial manure of the present invention on growth and yield of Chinese cabbage

Treatment zone	Plant height (cm)	Leaf width (cm)	Number of blades	Single plant weight (kg)
					Junf1 (bacterial manure)	35.1±0.30	49.7±0.50	15.7±0.30	1.9±0.23
Junf2 (bacterial manure)	37.0±0.80	48.5±0.70	18.9±0.40	1.7±0.10
					Junf3 (bacterial manure)	33.8±0.40	46.2±0.60	22.8±0.30	1.8±0.20
Ns1 (Urea)	31.5±0.40	39.5±0.30	16.2±0.20	1.4±0.30
					Ns2 (Urea)	34.6±0.50	40.2±0.50	14.5±0.40	0.9±0.15
Ns3 (Urea)	29.5±0.60	42.1±0.60	13.8±0.50	0.8±0.23

The organic solid waste resource transformation bacterial manure is obviously better than a urea control group from the yield of the harvested plot, and reaches the level of significant difference.

The organic solid waste resource transformation bacterial fertilizer has great influence on the agronomic characters of the corn, the plant height, the ear position height, the stem thickness, the root length, the plant dry quality and the fresh quality of the corn treated by the 2 fertilizing modes in the table 2 are gradually increased along with the growth of the corn, and the ear position height is less than half of the plant height. The corn plants in the three growth periods have no obvious difference in plant height, single green leaf number, root length, dry quality and fresh quality of the corn plants treated by the bacterial manure and the control group in the jointing period, but the bacterial manure applied in the emasculation period and the filling period has obvious advantages compared with the control group and has obvious difference. The stem thickness and the lateral root number show significant difference when bacterial manure is applied in three growth periods of the corn compared with a control group, the ear position is high, and the ear position of the bacterial manure applying group is 7-9cm higher than that of the control group in the emergence of the tasseling period and the filling period, and the difference is significant.

Example 7 resource-converting organic waste into small-molecule biodegradable products, biological bacteria and organic solid waste resource-converting bacterial manure (bio-organic fertilizer) to be applied to soil improvement.

The organic solid waste resource transformation bacterial fertilizer is beneficial to the fact that the number of soil microorganisms is an important index for reflecting the quality level and the health degree of soil, and the microorganisms participate in the processes of material circulation and energy metabolism to improve the soil. The experiment takes 0-20cm of soil of plant rhizosphere, and measures the quantity of bacteria, fungi and actinomycetes, and the result is shown in table 3, the application of bacterial manure obviously changes the quantity of bacteria, fungi and actinomycetes in the soil, and the influence effect on different microorganisms is different. Compared with a control, the number of bacteria and actinomycetes is increased by 302.65% and 64.47% respectively after bacterial manure is applied, the total number of soil microorganisms is increased by 274.56%, and the difference is obvious. And the number of the fungi applied to the bacterial manure group is lower than that of the control group, and compared with the control group, the number of the soil fungi is reduced by 67.9 percent.

TABLE 3 Effect of different fertilization methods on three major classes of microorganisms in soil improvement