阅读说明：本技术 一种荧光免疫分析仪用精准定量装置 (Accurate quantification device for fluorescence immunoassay analyzer ) 是由 崔智龙 王洛 贾银梁 张林帅 赵健凯 林宇鹏 何万圆 于 2021-07-15 设计创作，主要内容包括：本发明公开了一种荧光免疫分析仪用精准定量装置,包括光线筒,所述光线筒的下端固定有存放筒和吸取筒,所述光线筒的上端固定有控制箱,所述存放筒的底部贯穿开设有排出孔,所述排出孔的内壁固定有排出管,所述吸取筒的底部贯穿固定有吸入管,所述吸取筒的内部设有吸取转移机构,所述存放筒的内部设有定位排出机构,所述光线筒的内部设有光线传播触发机构。优点在于：可将发射头高度的调整认为是对液面高度的放大调整,从而能够将较小的液面数据的调整放大,从而降低仪器整体设计精度要求,用过数据的放大调整,能够降低数据过小带来的操作误差,从而提高试液添加的精准度。(The invention discloses a precise quantifying device for a fluorescence immunoassay analyzer, which comprises a light cylinder, wherein the lower end of the light cylinder is fixedly provided with a storage cylinder and a suction cylinder, the upper end of the light cylinder is fixedly provided with a control box, the bottom of the storage cylinder is provided with a discharge hole in a penetrating way, the inner wall of the discharge hole is fixedly provided with a discharge pipe, the bottom of the suction cylinder is fixedly provided with a suction pipe in a penetrating way, the inside of the suction cylinder is provided with a suction transfer mechanism, the inside of the storage cylinder is provided with a positioning discharge mechanism, and the inside of the light cylinder is provided with a light propagation triggering mechanism. Has the advantages that: the height of the emission head can be adjusted to be an amplification adjustment of the liquid level, so that the adjustment of smaller liquid level data can be amplified, the requirement on the overall design precision of the instrument is lowered, the amplification adjustment of used data can reduce the operation error caused by too small data, and the accuracy of adding the test solution is improved.)

1. The utility model provides a fluorescence immunoassay appearance is with accurate proportioning device, includes a light section of thick bamboo (1), its characterized in that, the lower extreme of a light section of thick bamboo (1) is fixed with storage cylinder (2) and absorbs a section of thick bamboo (3), the upper end of a light section of thick bamboo (1) is fixed with control box (4), the bottom of storage cylinder (2) is run through and has been seted up discharge hole (5), the inner wall of discharge hole (5) is fixed with discharge pipe (6), the bottom of absorbing a section of thick bamboo (3) is run through and is fixed with suction tube (7), the inside of absorbing a section of thick bamboo (3) is equipped with absorbs the transfer mechanism, the inside of storage cylinder (2) is equipped with location discharge mechanism, the inside of a light section of thick bamboo (1) is equipped with light propagation trigger mechanism.

2. The precise quantifying device for the fluorescence immunoassay analyzer according to claim 1, wherein the absorbing and transferring mechanism comprises a push plate (9) connected with the bottom of the absorbing cylinder (3) through a return spring (8), the side wall of the storage cylinder (2) is provided with a transferring hole (10) communicated with the inside of the absorbing cylinder (3) in a penetrating way, the inner wall of the absorbing cylinder (3) is connected with a baffle (15) through a torsion spring in a rotating way, and the inner wall of the storage cylinder (2) is connected with a one-way plate (17) through a torsion spring in a rotating way.

3. The precise quantifying device for the fluorescence immunoassay analyzer according to claim 2, wherein the outer wall of the light tube (1) is rotatably connected with a rotating shaft (11) through a torsion spring, a pressure rod (12) is fixed on the upper circumferential side wall of the rotating shaft (11), a knocking block (13) is fixed on the lower circumferential side wall of the rotating shaft (11), a piezoelectric block (14) is fixed on the side wall of the absorbing tube (3), and electrorheological fluid is filled in the absorbing tube (3).

4. The precise quantifying device for the fluorescence immunoassay analyzer according to claim 2, wherein a pressure relief hole (16) is formed at the upper end of the baffle (15) in a penetrating manner, a pressure valve is arranged inside the pressure relief hole (16), and an electromagnetic valve is arranged inside the transfer hole (10).

5. The precise dosing device for the fluoroimmunoassay analyzer according to claim 1, wherein the positioning and discharging mechanism comprises a positioning plate (19) connected with the bottom inside the storage barrel (2) through an extrusion spring (18), the positioning plate (19) is connected with the inner wall of the storage barrel (2) in a sealing and sliding manner, and the upper end of the positioning plate (19) is a mirror surface.

6. The precise quantifying device for the fluorescence immunoassay analyzer according to claim 1, wherein the light propagation triggering mechanism comprises a receiving groove (20) formed in the inner wall of the light tube (1), a photoresistor (21) is fixed on the inner wall of the receiving groove (20), a motor (22) is fixed at the inner bottom of the light tube (1), a screw rod (23) is fixed on an output shaft of the motor (22), a sliding block (24) is connected to the side wall of the screw rod (23) in a threaded manner, and an emitting head (25) is fixed on the side wall of the sliding block (24).

Technical Field

The invention relates to the technical field of fluorescence detection, in particular to a precise quantitative device for a fluorescence immunoassay analyzer.

Background

The fluorescence immunoassay technology has the advantages of strong specificity, high sensitivity, good practicability and the like, so that the fluorescence immunoassay technology is used for measuring bioactive compounds with very low content, such as proteins, hormones, medicaments, microorganisms and the like;

when carrying out fluorescence immunoassay detection work, need draw the interpolation work to reagent, the mode of adding through the ration realizes the high-efficient accurate fluorescence immunoassay of reagent, realize the interpolation that draws of test solution through graduated flask and burette usually at present, because try instrument precision problems such as graduated flask, it is impaired to have led to the test solution to add the precision, current equipment only can observe the ration according to current scale, when facing the interpolation process that the precision needs to be high, often because the data is less and can't effectively read and measure, and then reduced whole quantitative precision, and current mostly through manual regulation and observation, human error is great.

Disclosure of Invention

The invention aims to solve the problem of low precision in the prior art, and provides a precise quantitative device for a fluorescence immunoassay analyzer.

In order to achieve the purpose, the invention adopts the following technical scheme: the utility model provides a fluorescence immunoassay appearance is with accurate proportioning device, includes a light section of thick bamboo, the lower extreme of a light section of thick bamboo is fixed with the storage cylinder and absorbs a section of thick bamboo, the upper end of a light section of thick bamboo is fixed with the control box, the bottom of storage cylinder is run through and has been seted up the discharge hole, the inner wall of discharge hole is fixed with the discharge pipe, the bottom of absorbing a section of thick bamboo is run through and is fixed with the suction pipe, the inside of absorbing a section of thick bamboo is equipped with absorbs transfer mechanism, the inside of storage cylinder is equipped with location discharge mechanism, the inside of a light section of thick bamboo is equipped with light propagation trigger mechanism.

In foretell fluorescence immunoassay appearance is with accurate proportioning device, absorb the transfer mechanism include with absorb a push pedal that the bottom passes through reset spring and is connected in the section of thick bamboo, the lateral wall of storage cylinder runs through the transfer hole of seting up with the inside intercommunication of an absorption section of thick bamboo, the inner wall of an absorption section of thick bamboo is connected with the baffle through the torsional spring rotation, the inner wall of storage cylinder is connected with the one-way plate through the torsional spring rotation.

In the above precise quantifying device for the fluorescence immunoassay analyzer, the outer wall of the optical fiber tube is rotatably connected with a rotating shaft through a torsion spring, a pressing rod is fixed on the circumferential side wall of the upper part of the rotating shaft, a knocking block is fixed on the circumferential side wall of the lower part of the rotating shaft, a piezoelectric block is fixed on the side wall of the absorbing tube, and electrorheological fluid is filled in the absorbing tube.

In foretell fluorescence immunoassay appearance is with accurate proportioning device, the upper end of baffle is run through and is seted up the pressure release hole, the inside in pressure release hole is equipped with the pressure valve, the inside in transfer hole is equipped with the solenoid valve.

In foretell fluorescence immunoassay appearance is with accurate proportioning device, location discharge mechanism include with the storage cylinder bottom through extrude the locating plate of spring coupling, the sealed sliding connection of inner wall of locating plate and storage cylinder, the upper end of locating plate is the mirror surface.

In foretell fluorescence immunoassay appearance is with accurate proportioning device, light propagation trigger mechanism is including seting up the receiving tank in light section of thick bamboo inner wall, the inner wall of receiving tank is fixed with the photo resistance, the interior bottom of light section of thick bamboo is fixed with the motor, the output shaft of motor is fixed with the lead screw, the lateral wall threaded connection of lead screw has the slider, the lateral wall of slider is fixed with the emission head.

Compared with the prior art, the invention has the advantages that:

1. according to the invention, by arranging the pressure lever and the knocking block, when the test solution is sucked, the force required by pressing the pressure lever is far less than the impact force of the knocking block, so that the labor intensity of a user is reduced while the test solution is effectively knocked, and the use is more labor-saving and convenient;

2. according to the invention, by arranging the pressure relief hole, when the addition of the test solution is finished, an external power supply is smooth for a power supply line of the electrorheological fluid, so that the electrorheological fluid is greatly expanded, the push plate moves downwards again to extrude the test solution in the suction tube, so that the test solution is greatly extruded to break through a threshold value of a pressure valve in the pressure relief hole, and then the test solution flows back through the suction tube, so that the residual of the test solution in the suction tube can be avoided, and the effective use of the test solution is ensured;

3. according to the invention, by arranging the emission head and the photoresistor, the speed of photoelectric transmission is far higher than the flow transfer speed of liquid, so that the electromagnetic valve in the transfer hole can be quickly and effectively closed, and the accuracy of the test solution amount in the storage cylinder is ensured;

4. according to the invention, the adjustment of the height of the emission head can be regarded as the amplification adjustment of the liquid level height, so that the adjustment of smaller liquid level data can be amplified, the requirement of the overall design precision of the instrument is reduced, the operation error caused by over-small data can be reduced by the amplification adjustment of the data, and the accuracy of adding the test solution is improved.

Drawings

FIG. 1 is a schematic structural diagram of a precise quantitative device for a fluorescence immunoassay analyzer according to the present invention;

FIG. 2 is a side view of a precise quantitative apparatus for a fluorescence immunoassay analyzer according to the present invention;

FIG. 3 is an enlarged view of portion A of FIG. 1;

FIG. 4 is a schematic structural diagram of another embodiment of a precise quantitative apparatus for a fluorescence immunoassay analyzer according to the present invention;

fig. 5 is a schematic structural diagram of another state of a precise quantitative device for a fluorescence immunoassay analyzer according to the present invention.

In the figure: the device comprises a light tube 1, a storage tube 2, a suction tube 3, a control box 4, a discharge hole 5, a discharge tube 6, a suction tube 7, a return spring 8, a push plate 9, a transfer hole 10, a rotating shaft 11, a pressure rod 12, a knocking block 13, a piezoelectric block 14, a baffle plate 15, a pressure relief hole 16, a one-way plate 17, an extrusion spring 18, a positioning plate 19, a receiving groove 20, a photoresistor 21, a motor 22, a screw rod 23, a slide block 24 and an emitter head 25.

Detailed Description

The following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Examples

Referring to fig. 1-5, a fluorescence immunoassay appearance is with accurate proportioning device, including a light section of thick bamboo 1, the lower extreme of a light section of thick bamboo 1 is fixed with storage cylinder 2 and absorbs a section of thick bamboo 3, the upper end of a light section of thick bamboo 1 is fixed with control box 4, the bottom of storage cylinder 2 is run through and has been seted up discharge hole 5, the inner wall of discharge hole 5 is fixed with discharge pipe 6, the inside solenoid valve that is equipped with of discharge hole 5 is used for controlling the emission of test solution, the bottom of absorbing a section of thick bamboo 3 is run through and is fixed with suction tube 7, the inside of absorbing a section of thick bamboo 3 is equipped with absorbs transfer mechanism, the inside of storage cylinder 2 is equipped with location discharge mechanism, the inside of a light section of thick bamboo 1 is equipped with light and propagates trigger mechanism.

The absorption and transfer mechanism comprises a push plate 9 connected with the bottom in the absorption barrel 3 through a return spring 8, a transfer hole 10 communicated with the interior of the absorption barrel 3 is formed in the side wall of the storage barrel 2 in a penetrating manner, the inner wall of the absorption barrel 3 is rotatably connected with a baffle 15 through a torsion spring, and the inner wall of the storage barrel 2 is rotatably connected with a one-way plate 17 through the torsion spring; the outer wall of the optical fiber tube 1 is rotatably connected with a rotating shaft 11 through a torsion spring, a compression rod 12 is fixed on the circumferential side wall of the upper part of the rotating shaft 11, a knocking block 13 is fixed on the circumferential side wall of the lower part of the rotating shaft 11, a piezoelectric block 14 is fixed on the side wall of the suction tube 3, electrorheological fluid is filled in the suction tube 3, the compression rod 12 is long and labor-saving to press, and the knocking block 13 can be easily driven to knock the piezoelectric block 14, so that the piezoelectric block 14 is squeezed, and the piezoelectric block 14 can output electric power to the electrorheological fluid; the upper end of baffle 15 runs through and has seted up pressure release hole 16, and the inside of pressure release hole 16 is equipped with the pressure valve, shifts the inside of hole 10 and is equipped with the solenoid valve.

The positioning and discharging mechanism comprises a positioning plate 19 connected with the inner bottom of the storage barrel 2 through an extrusion spring 18, the positioning plate 19 is in sealing sliding connection with the inner wall of the storage barrel 2, and the upper end of the positioning plate 19 is a mirror surface; light propagation trigger mechanism is including offering the receiving tank 20 in the 1 inner wall of light barrel, the inner wall of receiving tank 20 is fixed with photo resistance 21, the interior bottom of light barrel 1 is fixed with motor 22, the output shaft of motor 22 is fixed with lead screw 23, the lateral wall threaded connection of lead screw 23 has slider 24, slider 24's lateral wall is fixed with emission head 25, receiving tank 20 lower part opening is thin and narrow and be specific angle, and emission head 25 installation angle is fixed, emission head 25 launches infrared light, the resistance changes after photo resistance 21 receives light, thereby the opening and closing of the inside solenoid valve of control transfer hole 10.

In the invention, when in actual use, the amount of the test solution required to be measured is input into the operation equipment in the control box 4, the motor 22 can receive signals and automatically operate, and further the screw rod 23 is driven to rotate, so that the slider 24 drives the emitter 25 to move to a specified position, and due to the change of the position of the emitter 25, infrared light emitted by the emitter 25 cannot be effectively reflected to the photoresistor 21, so that the electromagnetic valve in the transfer hole 10 is in an open state, and the test solution can be added into the storage barrel 2;

through pressing and loosening the pressure lever 12, the torsion spring connected with the rotating shaft 11 in a matching mode drives the knocking block 13 to move, the pressure lever 12 is quickly pressed and released, the knocking block 13 effectively knocks the piezoelectric block 14 under the action of the elasticity of the torsion spring, the piezoelectric block 14 is pressed to generate electricity, the length of the pressure lever 12 is far larger than that of the knocking block 13, and therefore the force required by pressing the pressure lever 12 is far smaller than the force of impact of the knocking block 13 according to the lever law, so that the labor intensity of a user is reduced while effective knocking is achieved, and the use is more labor-saving and convenient;

along with the intermittent power generation of the piezoelectric block 14, the electrorheological fluid in the suction cylinder 3 is solidified and expanded after being electrified, and is liquefied and recovered after being powered off, so that in the process that a user continuously presses the pressure rod 12, the push plate 9 is continuously pushed by the solidification process of the electrorheological fluid, the reciprocating motion of the push plate 9 can be realized by matching with the reset spring 8, when the push plate 9 moves downwards, the test solution in the suction cylinder 3 is extruded to pass through the transfer hole 10 and enter the storage cylinder 2, so that the addition of the reagent is realized, and when the push plate 9 moves upwards, the lower space of the suction cylinder 3 is increased, so that the external test solution can be sucked into the suction cylinder 3 from the suction pipe 7, and further the continuous addition of the test solution is realized;

along with the continuous entering of the test solution into the storage barrel 2, the liquid level inside the storage barrel 2 continuously rises, the positioning plate 19 is pushed by the test solution to move upwards, so that the position of the light reflected by the light emitted by the emitter 25 on the upper part of the positioning plate 19 is continuously changed, until the light is added into the specified test solution amount, the position of the positioning plate 19 reaches the set position, the light of the emitter 25 can smoothly irradiate the photoresistor 21, so that the electromagnetic valve inside the transfer hole 10 is closed again, because the speed of photoelectric transmission is far greater than the flow transfer speed of the liquid, the electromagnetic valve inside the transfer hole 10 can be quickly and effectively closed, so that the accuracy of the test solution amount inside the storage barrel 2 is ensured, meanwhile, the power supply circuit of an external power source to the electrorheological fluid is unobstructed, the electrorheological fluid is greatly expanded, the push plate 9 moves downwards again to extrude and absorb the test solution inside the barrel 3, so that the test solution is greatly extruded to break through the threshold value of the pressure relief hole 16, the test solution flows back through the suction pipe 7, so that the residual of the test solution in the suction cylinder 3 can be avoided, the effective use of the test solution is ensured, and when the test solution in the storage cylinder 2 needs to be released, the test solution can be effectively discharged under the elastic action and the gravity action of the extrusion spring 18 only by opening the valve in the discharge hole 5;

due to the determination of the installation angle of the emitter head 25, even if the height of the emitter head 25 is continuously changed, when the emitted light is transmitted to the upper mirror surface of the positioning plate 19, the incident angle is determined, the opening angle of the receiving groove 20 is also determined, and the two angles have the same size, according to the law that the incident angle is equal to the reflection angle in the light specular reflection process, the reflected light of the light emitted from the emitting head 25 is always parallel to the opening of the receiving groove 20, that is, whether the light can be emitted into the receiving groove 20 or not, only the final position of the light falling point is controlled, the control of the part can be realized by only adjusting the height of the positioning plate 19, thereby adjusting the incident point of the light, namely, the emitting head 25 and the positioning plate 19 both move upward by a specific height to ensure the position of the incident point, so that the light can be accurately reflected into the receiving groove 20;

the falling point of the reflected light is taken as the center line of the reflected light, the reflected light can be regarded as a line segment in the area, the line segment, the center line of the upper part of the positioning plate 19 and the vertical line of the inner wall of the storage barrel 2 can form a right-angled triangle, the tangent value of the reflected angle is determined because the size of the reflected angle is determined, and the ratio of the reflection point of the ray of the upper part of the positioning plate 19 to the liquid level scale of the storage barrel 2 can be obtained by utilizing the tangent value of the reflected angle;

meanwhile, the incidence angle is also determined, so that the ratio of the height of the emitter 25 to the length of the incidence point on the positioning plate 19 can be obtained, the ratio of the height of the emitter 25 to the length of the incidence point on the positioning plate 19 can be known, the variable is the addition amount of the test solution, the dependent variable is the height of the emitter 25, and the dependent variable and the emitter 25 are in a linear relationship of multiple, so that the adjustment of the position of the emitter 25 can be easily obtained according to the amount of the required test solution, the internal equipment of the control box 4 can be easily designed and calculated, and the intelligent operation equipment such as a chip and the like in the control box 4 is the prior art and is not described in detail herein;

meanwhile, the adjustment of the height of the emission head 25 can be regarded as the amplification adjustment of the liquid level height, so that the adjustment of smaller liquid level data can be amplified, the requirement on the overall design precision of the instrument is lowered, the amplification adjustment of used data can reduce the operation error caused by undersize data, and the accuracy of adding the test solution is improved.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.