阅读说明：本技术 化学发光分析仪测光器 (Chemical luminous analyzer light detector ) 是由 王爱民 玄哲玉 于 2021-07-27 设计创作，主要内容包括：本发明公开了化学发光分析仪测光器,包括使样品和试剂混合并反应的反应容器、通过免疫学方法以发光量测量样品中规定项目的浓度的分析区和控制分析区的动作、算出、报告发光量的计算结果的控制区,所述分析区内设有反应台区,反应台区的外侧依次设有样品供料区、供料斗区、检测区、BF清洗区和试剂台区,反应台区内转动设有反应盘,反应台区内设有若干搅拌机,反应台区和供料斗区之间和检测区和BF清洗区之间均设有转移机构,BF清洗区和试剂台区之间、试剂台区和样品供料区之间和样品供料区和反应台区之间均设有移液器,所述检测区内设有测光器,通过采用小型的非接触式的旋转挡板可以提供小型、高可靠性、低成本的测光区。(The invention discloses a chemical luminescence analyzer photometer, which comprises a reaction vessel for mixing and reacting a sample and a reagent, an analysis area for measuring the concentration of a specified item in the sample by using an immunological method through luminescence, and a control area for controlling the action, calculation and reporting of the calculation result of the luminescence of the analysis area, wherein a reaction table area is arranged in the analysis area, a sample feeding area, a feeding hopper area, a detection area, a BF cleaning area and a reagent table area are sequentially arranged outside the reaction table area, a reaction disc is rotationally arranged in the reaction table area, a plurality of stirring machines are arranged in the reaction table area, transfer mechanisms are respectively arranged between the reaction table area and the feeding hopper area and between the detection area and the BF cleaning area, pipettors are respectively arranged between the BF cleaning area and the reagent table area, between the reagent table area and the sample feeding area and between the sample feeding area and the reaction table area, a photometer is arranged in the detection area, and a small-sized pipettor is provided by adopting a non-contact rotary baffle plate, High reliability, low cost photometry area.)

1. A chemical luminescence analyzer photometer comprises a reaction container (2), an analysis area (1) and a control area (70), and is characterized in that a reaction table area (20) is arranged in the analysis area (1), a sample feeding area (4), a feeding hopper area (3), a detection area (30), a BF cleaning area (50) and a reagent table area (6) are sequentially arranged on the outer side of the reaction table area (20), a reaction disc (21) is rotatably arranged in the reaction table area (20), a plurality of stirring machines are arranged in the reaction table area (20), transfer mechanisms are respectively arranged between the reaction table area (20) and the feeding hopper area (3) and between the detection area (30) and the BF cleaning area (50), pipettors are respectively arranged between the BF cleaning area (50) and the reagent table area (6), between the reagent table area (6) and the sample feeding area (4) and between the sample feeding area (4) and the reaction table area (20);

the detection area (30) comprises a detection area base (31), a PMT base (32) is installed on the detection area base (31), a cylindrical internal baffle (33) is dispersedly embedded into the PMT base (32) from the lower part, the internal baffle (33) is connected with a baffle motor (36) fixed in a motor base (35) below the PMT base (32) through a seam (34) combined with a detection plate (34a), in addition, a detection baffle (37) opposite to the detection plate (34a) is installed in the motor base (35), a photomultiplier (38) is installed on the side surface of the PMT base (32), the internal baffle (33) is located in the direction opposite to the photomultiplier (38), and is cut into a rectangular A openings (33 a);

the cover frame (40) is fixed on the PMT base (32) through screws, in addition, an arm motor (41) fixed in the PMT base (32) is arranged above a reaction vessel support (39), a cover arm (43) capable of rotating through an arm boss (42) is installed in a rotating shaft of the arm motor (41), a ring-mounted cover support (44) is fixed on the cover arm (43), an opening is formed in one side, opposite to the cover arm (43), of the ring-mounted cover support (44), the cover support (44) is installed in a cover cup (46) through three cover springs (45), an arm spring (49) is installed between the cover arm (43) and the PMT base (32), and an arm detection a (47) and an arm detection b (48) are installed on the PMT base (32).

2. The chemiluminescent analyzer light meter of claim 1 wherein the reaction vessel holder (39) of the circular opening (39a) opposite the center of the light receiving face of the photomultiplier tube (38) is pressed into and secured over the PMT base (32).

3. The chemiluminescent analyzer light detector of claim 1 wherein the reaction vessel holder (39) has a wheel-shaped rubber pad (41A) clamped around its outer circumference to block light.

4. The chemiluminescent analyzer light detector of claim 1 wherein a light blocking ring rubber gasket attached to the PMT base (32) aperture sidewall near the lower end of the internal baffle (33) is mounted.

5. The chemiluminescent analyzer light detector of claim 1 wherein the reaction vessel holder (39) is part of the PMT base (32).

6. The chemiluminescent analyzer light meter of claim 1 wherein the cover bracket (40) is a unitary structure with the reaction vessel bracket (39).

Technical Field

The invention relates to the technical field of immunoassay analyzers, in particular to a light detector of a chemiluminescence analyzer.

Background

An immunoassay analyzer mixes a minute amount of a biological sample with a plurality of reagents including magnetic particles and an enzyme marker, causes an antigen-antibody reaction, adds a chemiluminescent substrate, and performs immunoassay based on the intensity of luminescence. The reaction and luminescence are carried out in a reaction container, the reaction container is placed in a light measuring area in a closed light-blocking environment, and the luminescence intensity is detected through a photomultiplier tube.

The importance of the light measuring area for measuring the luminous intensity in the immunoassay analyzer is undoubted, and the existing light measuring area has low stability, low measurement precision, large size of the analyzer and high cost. Accordingly, the present invention provides a chemiluminescent analyzer light detector that solves the problems set forth in the background above.

Disclosure of Invention

The present invention is directed to a chemiluminescence analyzer light detector, which solves the problems of the background art mentioned above.

In order to achieve the purpose, the invention provides the following technical scheme:

a chemiluminescence analyzer photometer comprises a reaction vessel for mixing and reacting a sample and a reagent, an analysis region for measuring the concentration of a predetermined item in the sample by an immunological method with a luminescence amount, and a control region for controlling the operation of the analysis region and calculating and reporting the result of the calculation of the luminescence amount, wherein a series of operations of the analysis region and the control region are automatically performed, and a plurality of immunological steps corresponding to the measurement items are programmed in an immunoassay analyzer.

Be equipped with reaction platform district in the analysis zone, the outside in reaction platform district is equipped with sample feed district in proper order, the feed fill district, the detection zone, BF rinsing area and reagent platform district, reaction platform district internal rotation is equipped with the reaction dish, be equipped with a plurality of mixers in the reaction platform district, all be equipped with transfer mechanism between reaction platform district and the feed fill district and between detection zone and the BF rinsing area, between BF rinsing area and the reagent platform district, between reagent platform district and the sample feed district and all be equipped with the pipettor between sample feed district and the reaction platform district.

The detection area comprises a detection area base, a PMT base is arranged on the detection area base, a cylindrical internal baffle is dispersedly embedded into the PMT base from the lower part with a tiny gap, the internal baffle is connected with a baffle motor fixed in the motor base below the PMT base through a seam combining with a detection plate a, in addition, the motor base is internally provided with the detection baffle opposite to the detection plate a, the side surface of the PMT base is provided with a photomultiplier, the internal baffle is positioned in the direction opposite to the photomultiplier, and a part of the internal baffle is cut into a rectangular A opening a;

the cover frame is fixed by screws on the basis of the PMT, in addition, an arm motor fixed in the PMT foundation is arranged above the reaction container support, a cover arm capable of rotating through an arm boss is installed in a rotating shaft of the arm motor, a ring-mounted cover support is fixed on the cover arm and provided with an opening on one side opposite to the cover arm, the cover support is installed in a cover cup through three cover springs, the arm springs are installed between the cover arm and the PMT foundation, an arm detection a and an arm detection b are installed on the PMT foundation, and the rotating position of the cover arm is detected through the arm detection a and the arm detection b installed on the arm boss.

In a further embodiment of the present invention, the reaction vessel holder having a circular opening a opposite to the center of the light-receiving surface of the photomultiplier tube is pressed into and fixed above the PMT base.

As a further proposal of the invention, a wheel-shaped rubber pad A for blocking light is clamped on the outer ring of the reaction vessel bracket.

As a further proposal of the invention, a light-blocking annular rubber gasket connected with the side wall of the PMT basic hole is arranged near the lower end of the internal baffle.

As a still further aspect of the invention, the reaction vessel holder is considered to be part of the PMT base.

As a further proposal of the invention, the cover frame and the reaction vessel bracket are of an integrated structure.

Compared with the prior art, the invention has the beneficial effects that:

a cap which is floatingly supported by three springs is provided on a cylindrical rotary shutter for making incident light to a photomultiplier and a rotary cap for attaching or detaching a reaction vessel, and external light can be blocked by the cap, and a small-sized, highly reliable, low-cost photometric area can be provided by using a small-sized non-contact rotary shutter.

Drawings

FIG. 1 is a schematic diagram of the structure of the analysis zone in a chemiluminescent analyzer light meter.

Fig. 2 is a schematic view of the structure of a detection baffle in a chemiluminescence analyzer light detector.

FIG. 3 is a schematic diagram of the structure of an arm spring in a light detector of a chemiluminescence analyzer.

FIG. 4 is a schematic diagram of a lid cup in a light detector of a chemiluminescence analyzer.

In the figure: 1. an analysis zone; 2. a reaction vessel; 3. a feed hopper zone; 4. a sample feed zone; 50. a BF washing area; 51. BF washes the disc; 6. a reagent station area; 20. a reaction platform area; 21. a reaction disc; 70. a control area;

30. a detection zone; 31. a test area base; 32. PMT basis; 33. an internal baffle; 33a, opening A; 34. seaming; 34a, a detection plate; 35. a motor foundation; 36. a baffle motor; 37. detecting a baffle; 38. a photomultiplier tube; 39. a reaction vessel holder; 39a, a circular opening;

40. a cover frame; 41. an arm motor; 41A, wheel-shaped rubber pads; 41B, a gasket; 42. an arm boss; 43. a cover arm; 44. a cover bracket; 45. a cover spring; 46. covering the cup; 47. an arm detection a; 48. an arm detection b; 49. an arm spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 to 4, in the embodiment of the present invention, the photometric device of the chemiluminescence analyzer includes a reaction vessel 2 for mixing and reacting a sample and a reagent, an analysis region 1 for measuring a concentration of a predetermined item in the sample by an immunological method, and a control region 70 for controlling the operation of the analysis region 1 and calculating and reporting a result of the calculation of the luminescence amount, wherein a series of operations of the analysis region 1 and the control region 70 are automatically performed, and a plurality of immunological steps corresponding to the measurement items are programmed in the immunoassay analyzer.

Be equipped with reaction platform district 20 in the analysis zone 1, the outside in reaction platform district 20 is equipped with sample feed district 4 in proper order, the feed fill district 3, the detection zone 30, BF washs district 50 and reagent platform district 6, 20 internal rotations in reaction platform district are equipped with reaction disc 21, be equipped with a plurality of mixers in the reaction platform district 20, all be equipped with the transfer mechanism between reaction platform district 20 and the feed fill district 3 and between detection zone 30 and BF washs district 50, between BF washs district 50 and reagent platform district 6, between reagent platform district 6 and the sample feed district 4 and all be equipped with the pipettor between sample feed district 4 and the reaction platform district 20.

The detection zone 30 includes a detection zone base 31, a PMT base 32 mounted on the detection zone base 31, a cylindrical internal baffle 33 dispersed with a slight gap from below embedded in the PMT base 32, the internal baffle 33 is connected to a baffle motor 36 fixed in a motor base 35 below the PMT base 32 through a joint 34 incorporating a detection plate 34a, in addition, a detection baffle 37 is installed in the motor base 35 opposite to the detection plate 34a, a photomultiplier tube 38 is installed on the side of the PMT base 32, the internal baffle 33 is positioned opposite to the photomultiplier tube 38, a portion of which is cut into a rectangular a-opening 33a, and a light-blocking ring-shaped rubber gasket (not shown) attached to the side wall of the hole of the PMT base 32 near the lower end thereof, on the other hand, in order to fit into the hollow hole of the internal baffle plate 33, a reaction vessel holder 39 having a circular opening 39a opposite to the center of the light receiving surface of the photomultiplier tube 38 is pressed and fixed above the PMT base 32;

the reaction vessel support 39 clamps a wheel-shaped rubber pad 41A for blocking light on the outer ring, the PMT base 32 fixes the cover frame 40 by screws, in addition, an arm motor 41 fixed in the PMT base 32 is arranged above the reaction vessel support 39, a cover arm 43 which can rotate by an arm boss 42 is arranged in the rotating shaft of the arm motor 41, a ring-mounted cover support 44 is fixed on the cover arm 43, the ring-mounted cover support 44 is provided with an opening on the side opposite to the cover arm 43, the cover support 44 is arranged in a cover cup 46 by 3 cover springs 45, an arm spring 49 is arranged between the cover arm 43 and the PMT base 32, an arm detection a47 and an arm detection b48 are arranged on the PMT base 32, and the rotating position of the cover arm 43 is detected by an arm detection a47 and an arm detection b48 which are arranged on the arm boss 42.

The working principle of the invention is as follows:

in the analysis region 1, the reaction vessel 2 is moved from the feed hopper region 3 to the reaction disk 21 at the position of the reaction platform region 20 by the transfer mechanism, then the position is moved by the counterclockwise rotation of the reaction disk 21, a sample is dispensed into the reaction vessel 2 by a pipette, a reagent containing magnetic particles of solid phase carrier is dispensed into the reaction vessel 2 by a pipette, then the reaction vessel 2 is rotated counterclockwise, and a reagent such as a buffer is added by a pipette. In the next cycle, the reaction plate 21 is moved 1 hole site, and the reaction vessel 2 is moved to a position and stirred by a stirrer.

The analysis section 1 repeats this operation, and after a predetermined reaction time, the reaction vessel 2 continues to rotate counterclockwise, where the labeled reagent is added by a pipette, and is stirred by another stirrer in the next cycle, and after the predetermined reaction time, the reaction vessel 2 moves in position in the operation cycle, is transferred to the BF washing section 50 by the transfer mechanism, is transferred to the BF washing section 50, is moved to the BF washing plate 51 in the BF washing section 50, and the BF washing plate 51 moves 1 well position every 1 cycle, and repeats this operation. In the BF washing section 50, the following operations are repeatedly carried out for the reaction liquid in the reaction vessel 2: magnetic particles are attracted by a magnet, cleaning liquid is attracted or discharged by a cleaning nozzle, and stirring is performed by BF cleaning stirring.

At the end of the cleaning, only the magnetic particles remain inside the reaction vessel 2. In the next cycle, the reaction vessel 2 is moved to a position where the substrate solution containing the chemiluminescent substrate is discharged through the substrate solution nozzle, stirred, and subjected to the secondary reaction, and after reaching an appropriate cycle, the reaction vessel 2 is moved to the detection region 30 by the transfer mechanism, and the light emission intensity is measured.

Next, the structure of the detection zone 30 of the present invention will be described. A cylindrical inner baffle 33 is dispersedly fitted into the PMT base 32 from below with a slight gap, the inner baffle 33 is connected to a baffle motor 36 fixed to a motor base 35 below the PMT base 32 through a joint 34 that joins a detection plate 34a, a detection baffle 37 is mounted in the motor base 35 so as to oppose the detection plate 34a, a photomultiplier tube 38 is mounted to the side of the PMT base 32, the inner baffle 33 is positioned in a direction opposing the photomultiplier tube 38, a portion of which is cut into a rectangular a opening 33a, and a light-blocking annular rubber gasket (not shown) is mounted near the lower end thereof so as to abut against the side wall of the hole of the PMT base 32, and a reaction vessel holder 39 having a circular opening 39a opposite to the center of the light-receiving surface of the photomultiplier tube 38 is pressed into and fixed above the PMT base 32 so as to fit into the hollow hole of the inner baffle 33;

the reaction vessel support 39 clamps a wheel-shaped rubber pad 41A for blocking light on the outer ring, the PMT base 32 fixes the cover frame 40 by screws, in addition, an arm motor 41 fixed in the PMT base 32 is arranged above the reaction vessel support 39, a cover arm 43 which can rotate by an arm boss 42 is arranged in the rotating shaft of the arm motor 41, a ring-mounted cover support 44 is fixed on the cover arm 43, the ring-mounted cover support 44 is provided with an opening on the side opposite to the cover arm 43, the cover support 44 is arranged in a cover cup 46 by 3 cover springs 45, an arm spring 49 is arranged between the cover arm 43 and the PMT base 32, an arm detection a47 and an arm detection b48 are arranged on the PMT base 32, and the rotating position of the cover arm 43 is detected by an arm detection a47 and an arm detection b48 which are arranged on the arm boss 42.

Although the detection region 30 is measured by the photomultiplier tube 38 using the counting method, since the amount of luminescence generated by the chemiluminescence method is very weak, it is necessary to completely block external light during measurement, and in addition, if the photoelectric surface of the photomultiplier tube 38 is subjected to strong light, the function may be damaged or the function may be restored within a certain time, so that when the reaction vessel 2 is moved to the detection region 30 or taken out from the photometric region, a light blocking protection measure needs to be applied to the photomultiplier tube 38 in order to prevent the external light.

Next, the operation of the detection section 30 will be described. FIG. 3 shows the state of the detection area 30 during measurement, in which a reaction vessel 2 containing a reaction solution is carried by a reaction vessel holder 39, and above the reaction vessel holder, the lower end of a cap cup 46 guided by a cap holder 44 is connected to the upper end of a spacer 41B fixed above a cap holder 40, and since the cap cup 46 is pressed by a cap spring 45 in a floating state at three points, the entire lower end surface of the cap cup 46 is in contact with the spacer 41B, and external light can be blocked completely, and the opening A33 a of an internal baffle plate 33 is located at a position opposite to the light-receiving surface of a photomultiplier tube 38, and light emitted from the reaction solution in the reaction vessel 2 reaches the photomultiplier tube 38 through the circular opening 39a of the reaction vessel holder 39, the opening A33 a of the internal baffle plate 33, and a window of a PMT base 32, and is measured; after the measurement of the amount of luminescence is completed, the internal baffle 33 is rotated by 180 ° by the baffle motor 36, and the opening a 33a of the internal baffle 33 is hidden in the reaction vessel holder 39 as viewed from the photomultiplier 38, and in this state, the cover cup 46 is rotated and moved by the counterclockwise rotation arm motor 41 to open the upper part of the reaction vessel holder 39, and the reaction vessel 2 whose measurement has been completed is taken out by the second transfer 10 and discarded into the reaction vessel disposal.

In addition to the cover cup 46, even if the reaction vessel holder 39 is dried under the external light, the light is injected only into the hole inside the reaction vessel holder 39, and the opening a 33a of the internal baffle 33 is hidden in the reaction vessel holder 39 when viewed from the photomultiplier tube 38, and even if there is a gap between the reaction vessel holder 39 and the internal baffle 33, and the PMT base 32, the external light does not become reflected light and does not affect the photomultiplier tube 38, next, the reaction vessel 2 for the next measurement is moved from the BF cleaning disk 51 to the detection area 30 by the second transfer 10, the detection area 30 is rotated by the arm motor 41 to return the cover cup 46 to above the reaction vessel holder 39 to block the external light, and the internal baffle 33 is rotated 180 ° so that the opening a 33a faces the photomultiplier tube 38 to perform photometry.

Although the reaction vessel holder 39 is illustrated as a separate component, the reaction vessel holder 39 may be used as part of the PMT base 32. In addition, even if the cover holder 40 is integrated with the reaction vessel holder 39, the effect of the present invention is not impaired.

The key point of the invention is that in the chemiluminescence immunoassay analyzer, the external light ray when the reaction container is put into or taken out of the light measuring area is shielded, and the luminous transmission of the reaction container is switched in the measuring process, and the reaction container is arranged in a cylindrical rotary baffle plate with a cut-off part of a window.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.