阅读说明：本技术 一种生物样本前处理系统 (Biological sample pretreatment system ) 是由 孔令和 朱斌 葛峰 于 2021-07-27 设计创作，主要内容包括：本发明公开了一种生物样本前处理系统,包括主支架,工作台设于主支架的底部,工作台上设有可在Y轴方向上移动的多个托盘,托盘上设有通用安装槽或吸头盒安装槽；移动座设于主支架上,并且可在X轴和Z轴方向上移动；吸引管通过吸附组件可上下移动的设于所述移动座的下方,用于吸附吸头；推针通过推动机构可上下移动的设置在所述吸引管上方,用于驱动吸头吸液/排液；退吸头把手设于移动座的顶部,用于驱动吸引管松开吸头,本发明以实现对吸头的吸附,吸液,退液以及退出吸头等步骤,移液效率和准确性高,样品处理的稳定性和可重复性高,能够满足多重移液的需要,整个过程的操作安静,不会产生噪音,给实验室营造一个良好的氛围。(The invention discloses a pretreatment system for biological samples, which comprises a main support, wherein a workbench is arranged at the bottom of the main support, a plurality of trays capable of moving in the Y-axis direction are arranged on the workbench, and a general mounting groove or a suction head box mounting groove is arranged on each tray; the movable seat is arranged on the main bracket and can move in the X-axis direction and the Z-axis direction; the suction tube is arranged below the movable seat and can move up and down through the adsorption component and is used for adsorbing the suction head; the push needle is arranged above the suction tube and can move up and down through a pushing mechanism and is used for driving the suction head to suck liquid/discharge liquid; the suction head withdrawing handle is arranged at the top of the movable seat and used for driving the suction tube to loosen the suction head.)

1. A biological specimen pretreatment system, comprising:

a main support;

the workbench is arranged at the bottom of the main support, a plurality of trays capable of moving in the Y-axis direction are arranged on the workbench, and a general mounting groove or a suction head box mounting groove is formed in each tray;

the movable seat is arranged on the main bracket and can move in the X-axis direction and the Z-axis direction;

the suction tubes are arranged below the movable seat in a manner that the suction tubes can move up and down through an adsorption component and are used for adsorbing suction heads;

the push needle is arranged above the suction tube and can move up and down through a pushing mechanism and is used for driving the suction head to suck/discharge liquid;

and the suction head withdrawing assembly is arranged at the top of the movable seat and is used for driving the suction head to be separated from the suction tube.

2. The biological sample pretreatment system according to claim 1, wherein: the main bracket also comprises a measuring range control button which is used for controlling the liquid suction amount of the suction head each time.

3. The biological sample pretreatment system according to claim 1, wherein: the quantity of tray includes two sets of trays of parallel arrangement, and every group tray includes two trays that can be on Y axle synchronous motion.

4. The biological sample pretreatment system according to claim 1, wherein: the movable seat can be manually moved in the X-axis and Z-axis directions of the main bracket through the movable handle.

5. The biological sample pretreatment system of claim 4, wherein the pushing mechanism comprises:

the guide assembly is arranged in the moving seat and comprises an upper substrate and a lower substrate which are vertically connected through guide posts;

the movable plate is arranged between the upper substrate and the lower substrate and can move up and down through the lifting assembly; the ejector pin is arranged at the bottom of the moving plate;

the liquid suction handle is rotatably connected with the movable handle, and when the liquid suction handle is pressed down, the movable handle drives the lifting assembly to move downwards.

6. The biological sample pretreatment system of claim 5, wherein the lift assembly comprises:

the rack is arranged in the moving seat in a vertically movable manner, and the bottom of the rack penetrates through the upper base plate to be connected with the moving plate;

the gear, be equipped with on the rack rather than the gear of meshing, the gear is located on the removal handle.

7. The system of claim 4, wherein the suction assembly comprises a suction head mounting handle, and the suction head mounting handle drives the rotation members located at both sides of the movable base to rotate through the connecting bar; the other end of the rotating piece is connected with the first base plate; when the suction head mounting handle is pressed down, the rotating piece is driven to rotate, and the rotating piece synchronously drives the first substrate to move downwards; the suction tube is arranged below the first substrate.

8. The biological sample pretreatment system according to claim 1, wherein: a protective cover is arranged outside the movable seat.

9. The biological sample pretreatment system according to claim 1, wherein: the universal mounting groove is provided with a pore plate or a liquid storage box, and the suction head box mounting groove is internally provided with a suction head box.

10. The biological sample pretreatment system according to claim 1, wherein: the head component comprises:

the two suction head withdrawing handles are rotatably arranged on two sides of the top of the moving seat;

the position returning rod is arranged at the bottom of the suction head returning handle and extends downwards to sequentially penetrate through the upper substrate, the lower substrate and the first substrate;

the withdrawing plate is connected with the bottom of the withdrawing rod, and the plurality of suction tubes synchronously penetrate through the withdrawing plate;

when the two suction head withdrawing handles rotate relatively, the withdrawing rod moves downwards, and then the withdrawing plate is driven to press the suction head downwards to separate the suction head from the suction tube.

Technical Field

The invention belongs to a biological sample processing system, and particularly relates to a biological sample pretreatment system.

Background

With the rapid development of scientific technology, the research work of biological and chemical laboratories is no longer small-scale experiments with several samples around several devices, but large-scale experiments with multiple ways and diversification are performed by using various experimental techniques in the face of more complicated research objects and increasing sample quantities.

In the laboratory, need carry out the preliminary treatment to biological sample, need frequently move liquid and annotate the liquid action to reagent in the most step of preliminary treatment, and current move liquid process and accomplish by manual operation mostly, and efficiency is comparatively low and annotate liquid volume control accurate inadequately, moreover because manual operation brings many human errors for the liquid accuracy of moving of sample processing is low, and the probability of makeing mistakes is high, and the stability and the repeatability of sample processing are poor, can not satisfy the demand under the multiple liquid condition of moving.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a biological sample pretreatment system which has high pipetting efficiency and accuracy, high sample treatment stability and repeatability and can meet the requirement of multiple pipetting.

In order to achieve the purpose, the invention adopts the technical scheme that: a biological specimen pretreatment system comprising:

a main support;

the workbench is arranged at the bottom of the main support, a plurality of trays capable of moving in the Y-axis direction are arranged on the workbench, and a general mounting groove or a suction head box mounting groove is formed in each tray;

the movable seat is arranged on the main bracket and can move in the X-axis direction and the Z-axis direction;

the suction tubes are arranged below the movable seat in a manner that the suction tubes can move up and down through an adsorption component and are used for adsorbing suction heads;

the push needle is arranged above the suction tube and can move up and down through a pushing mechanism and is used for driving the suction head to suck/discharge liquid;

and the suction head withdrawing assembly is arranged at the top of the movable seat and is used for driving the suction head to be separated from the suction tube.

Furthermore, the main bracket also comprises a measuring range control button which is used for controlling the amount of the sucker sucking liquid each time.

Further, the number of the trays comprises two groups of trays arranged in parallel, and each group of trays comprises two trays capable of synchronously moving on the Y axis.

Further, the movable base can be manually moved in the X-axis and Z-axis directions of the main support through the movable handle.

Further, the pushing mechanism includes:

the guide assembly is arranged in the moving seat and comprises an upper substrate and a lower substrate which are vertically connected through guide posts;

the movable plate is arranged between the upper substrate and the lower substrate and can move up and down through the lifting assembly; the ejector pin is arranged at the bottom of the moving plate;

the liquid suction handle is rotatably connected with the movable handle, and when the liquid suction handle is pressed down, the movable handle drives the lifting assembly to move downwards.

Further, the lifting assembly comprises:

the rack is arranged in the moving seat in a vertically movable manner, and the bottom of the rack penetrates through the upper base plate to be connected with the moving plate;

the gear, be equipped with on the rack rather than the gear of meshing, the gear is located on the removal handle.

Furthermore, the adsorption component comprises a suction head mounting handle, and the suction head mounting handle drives rotating pieces positioned on two sides of the moving seat to rotate through a connecting strip; the other end of the rotating piece is connected with the first base plate; when the suction head mounting handle is pressed down, the rotating piece is driven to rotate, and the rotating piece synchronously drives the first substrate to move downwards; the suction tube is arranged below the first substrate.

Furthermore, a protective cover is arranged outside the movable seat.

Furthermore, a pore plate or a liquid storage box is arranged on the general mounting groove, and a suction head box is arranged in the suction head box mounting groove.

Further, the head withdrawing assembly comprises:

the two suction head withdrawing handles are rotatably arranged on two sides of the top of the moving seat;

the position returning rod is arranged at the bottom of the suction head returning handle and extends downwards to sequentially penetrate through the upper substrate, the lower substrate and the first substrate;

the withdrawing plate is connected with the bottom of the withdrawing rod, and the plurality of suction tubes synchronously penetrate through the withdrawing plate;

when the two suction head withdrawing handles rotate relatively, the withdrawing rod moves downwards, and then the withdrawing plate is driven to press the suction head downwards to separate the suction head from the suction tube.

Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:

the biological sample pretreatment system has the advantages of simple overall structure and convenient operation, can realize the steps of absorbing, withdrawing liquid, withdrawing the absorbing head and the like of the absorbing head, is free from hand contact in the whole process, has high liquid transfer efficiency and accuracy, has high stability and repeatability of sample treatment, can meet the requirement of multiple liquid transfer, is quiet in operation in the whole process, does not generate noise, creates a good atmosphere for a laboratory, and meets the requirement of biological sample treatment.

Drawings

The technical scheme of the invention is further explained by combining the accompanying drawings as follows:

FIG. 1 is a schematic structural diagram of the present invention;

FIG. 2 is a schematic structural view of the present invention with the orifice plate, reservoir and tip cassette loaded;

FIG. 3 is a schematic structural view of the present invention with the main support, tray, table and protective cover omitted;

FIG. 4 is a schematic structural view of FIG. 3 with the movable plate moved downward;

FIG. 5 is a schematic view of the internal structure of the present invention;

FIG. 6 is a schematic view of the structure of FIG. 5 with the lower substrate omitted;

wherein: the device comprises a main bracket 1, a workbench 2, a tray 3, a movable seat 4, a suction tube 5, a suction head withdrawing handle 8, a protective cover 9, an orifice plate 11, a liquid storage tank 12, a suction head box 13, a range control button 14, a tray handle 15, a thimble 60, an upper substrate 61, a lower substrate 62, a guide column 63, a movable plate 64, a liquid suction handle 65, a rack 66, a gear 67, a movable handle 68, a suction head mounting handle 70, a connecting strip 71, a rotating piece 72, a first substrate 73, a withdrawing rod 80 and a withdrawing plate 81.

Detailed Description

The invention is described in further detail below with reference to the figures and the embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not to be construed as limiting the invention.

Example one

Referring to fig. 1-4, a biological sample pretreatment system according to an embodiment of the present invention includes a main support 1, a work bench 2, a tray 3, a movable seat 4, a suction tube 5, a push pin 60, and a suction withdrawal head assembly.

The workbench 2 is arranged at the bottom of the main support 1, two trays 3 capable of moving in the Y-axis direction are arranged on the workbench 2, one tray 3 is a left tray, and the other tray is a right tray; be equipped with two general mounting grooves that are used for depositing reservoir 12, orifice plate 11 or other standard containers on the tray of a left side, be equipped with general mounting groove and suction head box mounting groove on the tray of the right side, all be equipped with tray handle 15 on left side tray and the tray of the right side, be convenient for with both on the direction of Y axle go up the removal.

In this embodiment, two hole plates 11 are placed on the general mounting groove on the left tray, and a liquid storage tank 12 and a suction head box 13 are respectively placed on the general mounting groove and the suction head box mounting groove on the right tray.

The movable seat 4 is installed on the main bracket 1 and can move in the directions of the X axis and the Z axis respectively through two sliding assemblies; wherein, the movable seat 4 is also provided with a protective cover 9 outside to protect the internal parts of the treatment system.

The push pin 60 can move up and down in the movable seat 4 through a push mechanism, and the push mechanism comprises a guide assembly arranged in the movable seat 4; the guide assembly includes an upper substrate 61 and a lower substrate 62 connected up and down by guide posts 63; the moving plate 64 is disposed between the upper substrate and the lower substrate to be movable up and down by the elevating assembly.

The guide columns 63 guide the up-and-down movement of the moving plate 64, and the push pins 60 having a mesh structure are disposed at the bottom of the moving plate 64.

The movable handle 68 is rotatably connected with the imbibing handle 65, and when the imbibing handle 65 is pressed down, the imbibing handle 65 drives the lifting assembly to move up and down through the movable handle 65; the lifting assembly comprises a rack 66 and a gear 67; the gear 67 is connected with the moving handle 68; the gear 67 is engaged with a rack 66, and the bottom of the rack 66 passes through the upper base plate 62 and is connected with the moving plate 64.

In addition, the moving handle 68 can be used manually to move the entire moving seat in the X-axis and the Z-axis.

The adsorption component sets up on removing seat 4, adsorption component 7 includes suction head installation handle 70, suction head installation handle 70 passes through connecting strip 71 and drives the rotation piece 72 that is located to remove seat 4 both sides and rotates, the other end that rotates piece 72 links to each other with first base plate 73, suction head installation handle 70 drives rotation piece 72 when pushing down and rotates, it reciprocates to drive first base plate 73 when rotating piece 72 rotates, thereby drive a plurality of pipes 5 that attract of first base plate 73 bottom and move down in step, attract pipe 5 to push down to the suction head of suction head box 13 in, thereby adsorb the suction head.

In operation, the first base plate 73 is first moved downward by the suction head mounting handle 70, so that the suction tube 5 is pressed downward into the suction head corresponding to the suction head case 13, and the suction head is sucked onto the suction tube 5.

Then the liquid suction handle 65 is pressed down, the liquid suction handle 65 is rotated by the moving handle 68 to drive the gear 67 to rotate on the rack 66, so that the rack 66 moves downwards, the push pin 60 on the moving plate 64 is synchronously driven to move downwards, and the push pin 60 moves downwards to synchronously drive the suction head to perform liquid suction operation on the solution in the liquid storage tank 12.

Referring to fig. 5-6, a suction head withdrawing assembly is further arranged in the biological sample pretreatment system, the suction head withdrawing assembly comprises suction head withdrawing handles 8 which are rotatably arranged on two sides of the top of the movable base 4, a withdrawing rod 80 is arranged at the bottom of each suction head withdrawing handle 8, the withdrawing rod 80 extends downwards to sequentially extend through the upper base plate, the lower base plate and the first base plate and then is connected with a withdrawing plate 81, the withdrawing plate 81 is arranged below the first base plate, and the suction tubes 5 sequentially pass through the withdrawing plate; when the two suction head withdrawing handles 8 approach each other and rotate, the withdrawing plate is driven by the withdrawing rod to move downwards, and the withdrawing plate 81 presses the suction head downwards, so that the suction head is separated from the suction tube.

The specific work flow is as follows:

the right tray is completely pulled out to an outer gear, then the corresponding porous suction head box and the corresponding liquid storage tank are placed into the general mounting groove and the suction head box mounting groove corresponding to the right tray, and the two pore plates are mounted on the left tray.

1. Mounting suction head

Holding the movable handle, moving the main support to the rightmost side, slowly moving downwards to align the suction tube with the suction head in the suction head box, keeping the movable handle at a pressing position, pressing the suction head mounting handle downwards with force, driving the suction tube on the first substrate to be pressed downwards into the suction head by the suction head mounting handle, and loosening the suction head mounting handle after the suction head is mounted, so that the suction head is mounted on the suction tube.

After the suction head is installed, the whole movable seat is stably and completely lifted by the movable handle, so that the suction head box can be taken away for next liquid suction operation.

2. Liquid suction

The reservoir and the pipette tip are aligned up and down while holding the pipette handle and the traveling handle, and the pipette handle is pressed to a first position by hand to expel air.

When the liquid suction handle is pressed down, two gears are arranged, the liquid suction handle is slowly pressed down by small force, when the liquid suction handle is about to reach the end, the liquid suction handle can be continuously pressed down by larger operation force, and at the moment, the first gear is reached; the operating force is increased to completely press the bottom to the second gear.

Continuously holding and holding the liquid suction handle and the movable handle at the same time, and gently moving the suction head downwards to ensure that the suction head is immersed below the liquid level, wherein the depth is generally controlled to be 2-4 mm; keeping the height of the liquid suction module unchanged, slowly releasing the liquid suction handle until the liquid suction module completely resets to finish liquid suction, and stably moving out the suction head upwards after waiting for 1-3 seconds to finish liquid suction.

3 liquid discharge

And moving the suction head filled with the solution to the upper part of the pore plate, holding the movable handle, and inserting the suction head into the proper height of the pore plate.

Continuously holding the movable handle, keeping the height of the suction head, holding the liquid suction handle, slowly pressing to a first gear, discharging most of the solution, staying for about 1 second, continuously pressing to a second gear, and completely discharging the solution; keeping for 1-3 seconds, slowly releasing the liquid suction handle to finish liquid discharge.

4. Withdrawing suction head

Firstly, a container for containing the suction head after the test is installed in a suction head box installation groove, the suction head and the tray are moved to enable the suction head box to be aligned with the suction head, then the movable handle is held, most of the suction head is lowered into the suction head box, the movable handle is continuously held, and meanwhile, the two suction head withdrawing handle handles are moved inwards to rotate, so that the suction head can be withdrawn into the suction head box.

The biological sample pretreatment system has the advantages of simple overall structure and convenient operation, can realize the steps of adsorbing, absorbing, withdrawing liquid, withdrawing the suction head and the like of the suction head, is free from hand contact in the whole process, has high liquid transfer efficiency and accuracy, has high stability and repeatability of sample treatment, can meet the requirement of multiple liquid transfer, is quiet in operation in the whole process, does not generate noise, creates a good atmosphere for a laboratory, and meets the requirement of biological sample treatment.

The above is only a specific application example of the present invention, and the protection scope of the present invention is not limited in any way. All the technical solutions formed by equivalent transformation or equivalent replacement fall within the protection scope of the present invention.