阅读说明：本技术 一种哮喘小鼠的模型制备雾化吸入装置及其方法 (Model preparation aerosol inhalation device and method for asthma mouse ) 是由 李良昌 宋艺兰 王重阳 延光海 于 2021-08-25 设计创作，主要内容包括：本发明涉及哮喘小鼠模型搭建技术领域,具体是一种哮喘小鼠的模型制备雾化吸入装置及其方法,包括培养箱本体,所述培养箱本体底部内壁的中央位置设有隔板,所述隔板内壁的中央位置设有安装槽,所述安装槽的内壁上设有调节机构,所述调节机构上设有导出管,所述导出管的出口处连接有雾化喷嘴,所述雾化喷嘴的内壁设为双层结构,所述双层结构的内部设有引导机构,所述培养箱本体的顶部外壁上设有顶盖,且顶盖的顶部外壁上分别设有供药机构和雾化发生机构,本发明能够依靠雾化喷嘴中导流扇的引导,气流可以沿着双层结构的雾化喷嘴内壁将引诱颗粒散发的气味从气孔吹出来,吸引小鼠靠近雾化喷嘴,针对性的提供小鼠药物吸收。(The invention relates to the technical field of asthma mouse model building, in particular to an asthma mouse model preparation atomization inhalation device and a method thereof, the asthma mouse model preparation atomization inhalation device comprises an incubator body, a partition plate is arranged at the central position of the inner wall of the bottom of the incubator body, a mounting groove is arranged at the central position of the inner wall of the partition plate, an adjusting mechanism is arranged on the inner wall of the mounting groove, a delivery pipe is arranged on the adjusting mechanism, an atomization nozzle is connected at the outlet of the delivery pipe, the inner wall of the atomization nozzle is of a double-layer structure, a guide mechanism is arranged inside the double-layer structure, a top cover is arranged on the outer wall of the top of the incubator body, and a medicine supply mechanism and an atomization generating mechanism are respectively arranged on the outer wall of the top cover. The mouse is attracted to the atomizing nozzle, and the drug absorption of the mouse is provided in a targeted mode.)

1. An asthma mouse model preparation aerosol inhalation device which is characterized in that: comprises an incubator body (1), a partition plate (10) is arranged at the central position of the inner wall of the bottom of the incubator body (1), a mounting groove is arranged at the central position of the inner wall of the partition plate (10), an adjusting mechanism is arranged on the inner wall of the mounting groove, a delivery pipe (28) is arranged on the adjusting mechanism, an atomizing nozzle (11) is connected at the outlet (23) of the delivery pipe (28), the inner wall of the atomizing nozzle (11) is of a double-layer structure, a guide mechanism is arranged inside the double-layer structure, a top cover (3) is arranged on the outer wall of the top of the incubator body (1), a medicine supply mechanism and an atomization generation mechanism are respectively arranged on the outer wall of the top cover (3), a blocking box (21) is arranged at the central position of the outer wall of the bottom of the top cover (3), a blocking plate (22) is arranged on the inner wall of the blocking box (21), and a knob (20) is arranged on the outer wall of one side of the incubator, knob (20) are connected in one side of shutoff board (22) through the transfer line, shutoff case (21) take place the mechanism with the atomizing and are linked together, be equipped with the inspection hole on the one side outer wall of incubator body (1), and be equipped with clear glass (8) on the inner wall in inspection hole, be equipped with two chamber doors (9) on the opposite side outer wall of incubator body (1), two chamber door (9) are located the both sides of baffle (10) respectively.

2. The model-prepared aerosol inhalation device of claim 1, wherein: adjustment mechanism includes motor (12), threaded rod (19) and thread bush installation pipe (18), and motor (12) set up the central point at incubator bottom outer wall, and threaded rod (19) pass through the bearing and connect the central point at incubator bottom inner wall, and thread bush installation pipe (18) set up on the outer wall of threaded rod (19), and the one end of motor (12) output shaft is connected in the bottom of threaded rod (19).

3. The model-prepared aerosol inhalation device of claim 1, wherein: the guiding mechanism comprises a flow guide fan (30), attracting particles (29) and air holes (31), the flow guide fan (30) is arranged on the inner wall of one side of the atomizing nozzle (11), the attracting particles (29) are arranged on the inner wall of the other side of the atomizing nozzle (11), the air holes (31) are arranged on the inner wall of the opposite side of the atomizing nozzle (11), and the air holes (31) are in a double-layer structure communicated with the atomizing nozzle (11).

4. The model-prepared aerosol inhalation device of claim 1, wherein: the medicine supply mechanism comprises a liquid storage box (5), a first support frame (4), an outer peripheral plate (2), an inner peripheral plate (33), a winding hose (32), a first motor (16), an elliptical block (34) and a connecting nozzle (35), wherein the liquid storage box (5) is arranged on the outer wall of the top of a top cover (3), the winding hose (32) is connected to the outlet (23) of the liquid storage box (5), the connecting nozzle (35) is connected to one end of the winding hose (32), the outer peripheral plate (2) and the inner peripheral plate (33) are both arranged on one side of the outer wall of the top cover (3), a gap is formed between the outer peripheral plate (2) and the inner peripheral plate (33), the winding hose (32) is positioned in the gap, the first support frame (4) is arranged on the outer wall of one side of the outer peripheral plate (2), the first motor (16) is arranged on the outer wall of the top of the first support frame (4), one end of an output shaft of the first motor (16) is connected with a rotating rod, the oval block (34) is arranged at the bottom end of the rotating rod.

5. The model-prepared aerosol inhalation device of claim 4, wherein: the inner wall of the inner enclosing plate (33) is provided with an adaptive hole, and the elliptical block (34) is adaptive to the adaptive hole.

6. The model-prepared aerosol inhalation device of claim 1, wherein: the atomization generating mechanism comprises a guide pipe (36), a contact spray head (37), an engraved groove (38), a rotary table (39), a second motor (17), a second support frame and an atomization box (6), the second support frame is arranged on one side of the outer wall of the top cover (3), the second motor (17) is arranged on the second support frame, one end of an output shaft of the second motor (17) is connected with a rotating shaft, the rotary table (39) is arranged at the bottom end of the rotating shaft, the rotary table (39) is of an inverted circular truncated cone structure, the engraved groove (38) is arranged around the outer wall of the bottom of the rotary table (39), the guide pipe (36) is arranged at one end of the connecting nozzle (35), the contact spray head (37) is arranged at one end of the guide pipe (36), and the contact spray head (37) is close to the notch groove (38), the atomization box is arranged at the central position of the outer wall of the top cover (3), and the rotary disc (39) is connected with the atomization box through a bearing.

7. The model-prepared aerosol inhalation device of claim 1, wherein: the both sides of the bottom outer wall of shutoff case (21) all are equipped with export (23), all be equipped with atomizing core (24) on the inner wall of two exports (23), be equipped with on the outer wall of atomizing core (24) and prevent liquefaction subassembly (14), be equipped with coupling hose (13) on one side outer wall of preventing liquefaction subassembly (14), the top at delivery tube (28) is connected to the one end of coupling hose (13), be equipped with torrent gasket (25) and mounting bracket on the inner wall of atomizing core (24), be connected with rotating fan blade (26) through the bearing on one side outer wall of mounting bracket, be equipped with on the outer wall that atomizing core (24) are close to one end spray orifice (27) that the equidistance distributes.

8. The model-prepared aerosol inhalation device of claim 1, wherein: one side of top cap (3) top outer wall is equipped with fan (7), and export (23) department of fan (7) is connected with connecting pipe (15), and the one end of connecting pipe (15) is connected in one side of atomizing box (6) top outer wall, and is connected with dispersion flabellum (40) through the bearing on the inner wall of atomizing box (6).

9. A use method of an asthma mouse model for preparing an aerosol inhalation device is characterized by comprising the following steps:

s1: firstly, research medicines are added into a liquid storage tank (5), then a motor (12) is started, the motor (12) is started to drive a threaded rod (19) to rotate, the threaded rod (19) rotates to drive a threaded sleeve mounting pipe (18) to adjust downwards, the motor (12) is closed until an atomizing nozzle (11) is lowered to a height which can be reached by a mouse, and meanwhile, a first motor (16), a second motor (17) and a fan (7) are started;

s2: then the first motor (16) drives the elliptical block (34) to rotate on the inner wall of the inner enclosing plate (33), the creeping type extrudes the winding hose (32), so that the medicine flows from the liquid storage box (5) to the contact spray head (37) on one side of the conduit (36), the second motor (17) drives the rotary disc (39) to rotate, the notch (38) on the rotary disc (39) rotates along with acceleration, when the notch (38) is contacted with the medicine liquid flow on one side of the contact spray head (37), a centrifugal atomization effect is generated under the action of centrifugal motion, the fan (7) blows the dispersing fan blades (40) in the atomization box (6) through the connecting pipe (15), the generated atomized medicine enters the blockage box, and the switch of two outlets (23) of the blockage box can be adjusted through the knob (20);

s3: then after the liquid mist enters the anti-liquefaction component (14) from the outlet (23), under the effect of the rapid flow gasket (25), the rotating fan blades (26) are accelerated to rotate, the large liquid drops converged into particles are broken up again and sprayed out from the spray holes (27), and the sprayed atomized medicine enters the atomizing nozzle (11) through the connecting hose (13);

s4: finally, the atomized air flow enters the double-layer structure from the gap at one side of the atomizing nozzle (11), and under the guidance of the guide fan (30), the odor emitted by the luring particles (29) is blown out from the air holes (31) to attract the mice to be close to the atomizing nozzle (11), and the atomized medicine is actively absorbed from the atomizing nozzle (11).

Technical Field

The invention relates to the technical field of establishment of asthma mouse models, in particular to an asthma mouse model preparation aerosol inhalation device and a method thereof.

Background

The construction of mouse allergic asthma model usually includes two parts of sensitization and challenge. The common sensitization modes include subcutaneous injection, intraperitoneal injection, direct contact of respiratory tract and the like, and the excitation modes include nasal drip, atomization and the like. Different sensitization and priming regimens will affect the immune response of the mice to some extent. Allergic asthma is a complex multifactorial disease caused by inhalation of allergens, and the incidence rate is increasing globally, allergic asthma is a chronic airway inflammation involving various cells, particularly mast cells, eosinophils and T lymphocytes, which can cause recurrent wheezing, shortness of breath, chest tightness and/or cough in susceptible people, and is mostly caused at night and/or in the morning, and the airway has increased reactivity to various stimulators. But the symptoms can be relieved by themselves or by treatment. In the recent more than one decade, the prevalence and mortality of asthma in the united states, the united kingdom, australia, new zealand and other countries have an increasing trend, and about one hundred million asthmatics all over the world have become a major chronic disease that seriously threatens public health. The drug experiment using the mouse is one of the better choices, and when the mouse is used for the sensitization experiment, the mouse is mostly contacted through the respiratory tract, and the atomization is mostly adopted in the way of activation.

In the prior art, the following problems exist:

(1) most of the existing atomization absorption devices passively wait for mice to inhale after atomized medicines are filled in a culture space, and have poor pertinence, long medicine spraying time and serious leakage;

(2) in the existing atomization technology, the matching between the medicine supply and the atomization generator is poor, so that the atomization effect is easy to be poor;

(3) in the long pipeline transmission process of atomizing medicine, the condition that the atomizing granule condenses very fast can appear in most atomizing medicines and produce, and the liquefaction process becomes fast to also can cause the atomization effect variation.

Disclosure of Invention

The invention aims to provide an aerosol inhalation device prepared by a model of an asthmatic mouse and a method thereof, which aim to solve the problems in the background technology.

The technical scheme of the invention is as follows: an asthma mouse model preparation atomization inhalation device comprises an incubator body, wherein a partition plate is arranged at the central position of the inner wall of the bottom of the incubator body, a mounting groove is formed in the central position of the inner wall of the partition plate, an adjusting mechanism is arranged on the inner wall of the mounting groove, a delivery pipe is arranged on the adjusting mechanism, an atomization nozzle is connected to the outlet of the delivery pipe, the inner wall of the atomization nozzle is of a double-layer structure, a guide mechanism is arranged inside the double-layer structure, a top cover is arranged on the outer wall of the top of the incubator body, a medicine supply mechanism and an atomization generation mechanism are respectively arranged on the outer wall of the top cover, a plugging box is arranged at the central position of the outer wall of the bottom of the top cover, a plugging plate is arranged on the inner wall of the plugging box, a knob is arranged on the outer wall of one side of the incubator, the knob is connected to one side of the plugging plate through a transmission rod, and is communicated with the atomization generation mechanism, the incubator comprises an incubator body and is characterized in that an observation hole is formed in the outer wall of one side of the incubator body, transparent glass is arranged on the inner wall of the observation hole, two chamber doors are arranged on the outer wall of the other side of the incubator body, and the two chamber doors are located on two sides of a partition plate respectively.

Preferably, adjustment mechanism includes motor, threaded rod and thread bush installation pipe, and the motor setting is in the central point of incubator bottom outer wall position, and the threaded rod passes through the bearing and connects the central point at incubator bottom inner wall position, and the thread bush installation pipe sets up on the outer wall of threaded rod, and the one end of motor output shaft is connected in the bottom of threaded rod.

Preferably, the guide mechanism comprises a guide fan, an attracting particle and an air hole, the guide fan is arranged on the inner wall of one side of the atomizing nozzle, the attracting particle is arranged on the inner wall of the other side of the atomizing nozzle, the air hole is arranged on the inner wall of the opposite side of the atomizing nozzle, and the air hole is used for communicating with the double-layer structure of the atomizing nozzle.

Preferably, the medicine supply mechanism comprises a liquid storage box, a first support frame, an outer surrounding plate, an inner surrounding plate, a winding hose, a first motor, an oval block and a connecting nozzle, the liquid storage box is arranged on the outer wall of the top cover, the winding hose is connected to the outlet of the liquid storage box, the connecting nozzle is connected to one end of the winding hose, the outer surrounding plate and the inner surrounding plate are both arranged on one side of the outer wall of the top cover, a gap is formed between the outer surrounding plate and the inner surrounding plate, the winding hose is located inside the gap, the first support frame is arranged on the outer wall of one side of the outer surrounding plate, the first motor is arranged on the outer wall of the top of the first support frame, one end of an output shaft of the first motor is connected with the rotating rod, the oval block is arranged at the bottom end of the rotating rod, an adaptive hole is formed in the inner wall of the inner surrounding plate, and the oval block is adaptive to the adaptive hole.

Preferably, the mechanism includes the pipe, the contact nozzle, the cutting, the carousel, the second motor, second support frame and atomizing box take place, the second support frame sets up the one side at top cap top outer wall, the second motor sets up on the second support frame, the one end of second motor output shaft is connected with the pivot, the carousel sets up the bottom in the pivot, the carousel sets up to invert the round platform structure, the cutting sets up around carousel bottom outer wall, the pipe sets up the one end at the connection mouth, the contact nozzle sets up the one end at the pipe, and the contact nozzle is close to mutually with the cutting, the atomizing box sets up the central point at top cap top outer wall, the carousel is connected through the bearing with the atomizing box.

Preferably, the both sides of the bottom outer wall of shutoff case all are equipped with the export, all are equipped with the atomizing core on the inner wall of two exports, are equipped with on the outer wall of atomizing core and prevent the liquefaction subassembly, are equipped with coupling hose on one side outer wall of preventing the liquefaction subassembly, and coupling hose's one end is connected on the top of contact tube, is equipped with torrent gasket and mounting bracket on the inner wall of atomizing core, is connected with rotating fan blade through the bearing on one side outer wall of mounting bracket, is equipped with the orifice that the equidistance distributes on the outer wall that the atomizing core is close to one end, one side of top cap top outer wall is equipped with the fan, and the exit of fan is connected with the connecting pipe, and the one end of connecting pipe is connected in one side of atomizing box top outer wall, and is connected with dispersion flabellum through the bearing on the inner wall of atomizing box.

The invention provides a use method of an asthma mouse model-prepared aerosol inhalation device, which comprises the following steps:

s1: firstly, adding a research drug into a liquid storage box, then starting a motor, starting the motor to drive a threaded rod to rotate, driving a threaded sleeve mounting pipe to adjust downwards by rotating the threaded rod until an atomizing nozzle is lowered to a height which can be reached by a mouse, closing the motor, and simultaneously starting a first motor, a second motor and a fan;

s2: the first motor drives the elliptical block to rotate on the inner wall of the inner enclosing plate, the creeping type extrudes the winding hose to enable the medicine to flow from the liquid storage box to the contact spray head on one side of the catheter, the second motor drives the rotary table to rotate, so that the notch on the rotary table rotates along with acceleration, when the notch is contacted with the medicine liquid flow on one side of the contact spray head, a centrifugal atomization effect starts to be generated under the action of centrifugal motion, the fan blows dispersing fan blades in the atomization box through the connecting pipe to enable the generated atomized medicine to enter the plugging box, and the switches of two outlets of the plugging box can be adjusted through the knob;

s3: then after the liquid mist enters the anti-liquefaction component from the outlet, the rotating fan blades are accelerated to rotate under the effect of the rapid flow gasket, the large liquid drops which are gathered into particles are broken up again and sprayed out from the spray holes, and the sprayed atomized medicine enters the atomizing nozzle through the connecting hose;

s4: and finally, the atomized air flow enters the double-layer structure from the gap on one side of the atomizing nozzle, and under the guidance of the flow guide fan, the odor which is used for attracting the particles to disperse is blown out from the air holes, so that the mouse is attracted to be close to the atomizing nozzle, and the atomized medicine is actively absorbed from the atomizing nozzle.

The invention provides an aerosol inhalation device prepared by improving a model of an asthma mouse and a method thereof, and compared with the prior art, the aerosol inhalation device has the following improvements and advantages:

one is as follows: according to the invention, the atomizing nozzle is arranged and is of a double-layer structure, the flow guide fan, the inducing particles and the air holes are arranged in the double-layer structure, after atomized air flow enters the double-layer structure from a gap at one side of the atomizing nozzle, under the guidance of the flow guide fan, the air flow can blow out smell emitted by the inducing particles from the air holes along the inner wall of the atomizing nozzle of the double-layer structure, so that a mouse is attracted to be close to the atomizing nozzle, and the drug absorption of the mouse is pertinently provided;

the second step is as follows: according to the invention, the outer surrounding plate, the inner surrounding plate and the elliptical block are arranged, the elliptical block is driven to rotate after the first motor is started, the elliptical block is connected to the inner wall of the inner surrounding plate in a sliding manner, the winding hose is positioned in a gap between the inner surrounding plate and the outer surrounding plate, a peristaltic liquid supply mode is formed under the rotating extrusion of two protruding positions of the elliptical block, and the slow liquid supply is stably matched with an atomization generating mechanism of the second motor, so that the medicinal liquid mist is generated more effectively.

And thirdly: according to the invention, the anti-liquefaction component and the atomization core are arranged, the fan blows the dispersing fan blades in the atomization box to rotate through the connecting pipe, the generated liquid mist is mixed and pushed, the generated atomized medicine can enter the plugging box, after the liquid mist enters the anti-liquefaction component from the outlet, the rotation fan blades are accelerated to rotate under the effect of the rapid flow gasket by matching with the fan, the large liquid drops formed by aggregation are dispersed again, the large liquid drops are sprayed out from the spray hole, the sprayed atomized medicine enters the atomization nozzle through the connecting hose, and the anti-liquefaction component is arranged at the middle section of the transmission position, so that the atomization form of the medicine is stably maintained.

Drawings

The invention is further explained below with reference to the figures and examples:

FIG. 1 is a perspective view of the present invention;

FIG. 2 is a front view of the present invention;

FIG. 3 is a perspective view of a separator plate according to the present invention;

FIG. 4 is a schematic view of the construction of the inventive closure;

fig. 5 is a perspective view of the liquefaction prevention assembly of the present invention.

Fig. 6 is a schematic view of the structure of the torrent gasket of the invention.

Fig. 7 is a schematic perspective view of the inside of the atomizing nozzle of the present invention.

Fig. 8 is a schematic perspective view of the drug delivery mechanism of the present invention.

Fig. 9 is a schematic perspective view of the atomization generating mechanism of the present invention.

Fig. 10 is a schematic view of the inner structure of the atomizing cartridge of the present invention.

Description of reference numerals:

1. an incubator body; 2. a peripheral plate; 3. a top cover; 4. a first support frame; 5. a liquid storage tank; 6. an atomization cartridge; 7. a fan; 8. transparent glass; 9. a box door; 10. a partition plate; 11. an atomizing nozzle; 12. a motor; 13. a connecting hose; 14. a liquefaction-resistant component; 15. a connecting pipe; 16. a first motor; 17. a second motor; 18. mounting a pipe by a threaded sleeve; 19. a threaded rod; 20. a knob; 21. plugging the box; 22. a plugging plate; 23. an outlet; 24. an atomizing core; 25. a rapid flow gasket; 26. rotating the fan blades; 27. spraying a hole; 28. a delivery pipe; 29. attracting particles; 30. a flow guiding fan; 31. air holes; 32. a winding connection hose; 33. an inner coaming; 34. an elliptical block; 35. a connecting nozzle; 36. a conduit; 37. contacting the spray head; 38. grooving; 39. a turntable; 40. dispersing the fan blades.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention.

The components of the patented embodiments of the invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present patent, presented in the figures, is not intended to limit the scope of the patent, as claimed, but is merely representative of selected embodiments of the patent.

All other embodiments obtained by a person skilled in the art based on the embodiments in the patent of the invention without any inventive work belong to the protection scope of the patent of the invention.

In the description of the present patent, it is to be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the present patent and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present patent. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present patent application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present patent can be understood in a specific case by those skilled in the art.

It is well within the skill of those in the art to implement, without undue experimentation, the subject invention as claimed and not related to software and process improvements.

The invention provides an aerosol inhalation device prepared by improving a model of an asthma mouse and a method thereof, and the technical scheme of the invention is as follows:

as shown in fig. 1-10, an asthma mouse model preparation aerosol inhalation device comprises an incubator body 1, a partition plate 10 is disposed at the central position of the inner wall of the bottom of the incubator body 1, a mounting groove is disposed at the central position of the inner wall of the partition plate 10, an adjusting mechanism is disposed on the inner wall of the mounting groove, a delivery pipe 28 is disposed on the adjusting mechanism, an atomizing nozzle 11 is connected to an outlet 23 of the delivery pipe 28, the inner wall of the atomizing nozzle 11 is a double-layer structure, a guiding mechanism is disposed inside the double-layer structure, a top cover 3 is disposed on the outer wall of the top of the incubator body 1, a drug supply mechanism and an aerosol generation mechanism are disposed on the outer wall of the top cover 3, a blocking box 21 is disposed at the central position of the outer wall of the bottom of the top cover 3, a blocking plate 22 is disposed on the inner wall of the blocking box 21, a knob 20 is disposed on the outer wall of one side of the incubator, the knob 20 is connected to one side of the blocking plate 22 through a transmission rod, the blocking box 21 is communicated with the atomization generating mechanism, an observation hole is formed in the outer wall of one side of the incubator body 1, transparent glass 8 is arranged on the inner wall of the observation hole, two box doors 9 are arranged on the outer wall of the other side of the incubator body 1, the two box doors 9 are located on two sides of the partition plate 10 respectively, main processes of medicine supply, atomization and transmission steps are completed by means of contact among all the mechanisms, and the research on medicine action of a mouse is pertinently carried out.

Further, the adjusting mechanism comprises a motor 12, a threaded rod 19 and a threaded sleeve mounting tube 18, the motor 12 is arranged at the central position of the outer wall of the bottom of the incubator, the threaded rod 19 is connected at the central position of the inner wall of the bottom of the incubator through a bearing, the threaded sleeve mounting tube 18 is arranged on the outer wall of the threaded rod 19, one end of an output shaft of the motor 12 is connected with the bottom end of the threaded rod 19, the guiding mechanism comprises a flow guiding fan 30, an attracting particle 29 and an air hole 31, the flow guiding fan 30 is arranged on the inner wall of one side of the atomizing nozzle 11, the attracting particle 29 is arranged on the inner wall of the other side of the atomizing nozzle 11, the air hole 31 is arranged on the inner wall of the opposite side of the atomizing nozzle 11, the air hole 31 is used for communicating with the double-layer structure of the atomizing nozzle 11, the medicine supply mechanism comprises a liquid storage box 5, a first support frame 4, a peripheral plate 2, an inner surrounding plate 33, a surrounding hose 32, a first motor 16, an oval block 34 and a connecting nozzle 35, the liquid storage box 5 is arranged on the outer wall of the top cover 3, the wrapping hose 32 is connected to the outlet 23 of the liquid storage box 5, the connecting nozzle 35 is connected to one end of the wrapping hose 32, the outer surrounding plate 2 and the inner surrounding plate 33 are both arranged on one side of the outer wall of the top cover 3, a gap is arranged between the outer surrounding plate 2 and the inner surrounding plate 33, the wrapping hose 32 is positioned in the gap, the first support frame 4 is arranged on the outer wall of one side of the outer surrounding plate 2, the first motor 16 is arranged on the outer wall of the top of the first support frame 4, one end of the output shaft of the first motor 16 is connected with a rotating rod, the oval block 34 is arranged at the bottom end of the rotating rod, the inner wall of the inner surrounding plate 33 is provided with an adapting hole, the oval block 34 is adapted to the adapting hole, the atomization generating mechanism comprises a conduit 36, a contact spray nozzle 37, an engraved groove 38, a rotating disk 39, a second motor 17, a second support frame and an atomization box 6, the second support frame is arranged on one side of the outer wall of the top cover 3, second motor 17 sets up on the second support frame, the one end of second motor 17 output shaft is connected with the pivot, carousel 39 sets up the bottom in the pivot, carousel 39 sets up to invert round platform structure, notch 38 sets up around the outer wall of carousel 39 bottom, pipe 36 sets up the one end at connector 35, contact spray 37 sets up the one end at pipe 36, and contact spray 37 is close to with notch 38 mutually, the atomizer box sets up the central point at top 3 top outer wall, carousel 39 is connected through the bearing with the atomizer box, utilize atomizer 11 and the medicine supply mechanism who improves the development, it needs stable spraying to provide the mouse.

Furthermore, outlets 23 are arranged on two sides of the outer wall of the bottom of the blocking box 21, atomization cores 24 are arranged on the inner walls of the two outlets 23, anti-liquefaction assemblies 14 are arranged on the outer walls of the atomization cores 24, connecting hoses 13 are arranged on the outer wall of one side of each anti-liquefaction assembly 14, one end of each connecting hose 13 is connected to the top end of the corresponding guide pipe 28, a rush current gasket 25 and a mounting rack are arranged on the inner wall of each atomization core 24, rotating fan blades 26 are connected to the outer wall of one side of the mounting rack through bearings, spray holes 27 which are distributed equidistantly are arranged on the outer wall of the atomization core 24 close to one end of each atomization core, a fan 7 is arranged on one side of the outer wall of the top cover 3, a connecting pipe 15 is connected to the outlet 23 of the fan 7, one end of each connecting pipe 15 is connected to one side of the outer wall of the top of the atomization box 6, and dispersing fan blades 40 are connected to the inner wall of the atomization box 6 through bearings, so that stable transmission of atomized medicines is effectively maintained, and the anti-liquefaction assemblies 14 are matched, the degree of maintenance of atomization is ensured.

The invention provides a use method of an asthma mouse model-prepared aerosol inhalation device, which comprises the following steps:

s1: firstly, research medicines are added into a liquid storage box 5, then a motor 12 is started, the motor 12 is started to drive a threaded rod 19 to rotate, the threaded rod 19 rotates to drive a threaded sleeve mounting pipe 18 to adjust downwards, the motor 12 is closed after an atomizing nozzle 11 is lowered to a height which can be touched by a mouse, and meanwhile, a first motor 16, a second motor 17 and a fan 7 are started;

s2: the first motor 16 drives the elliptical block 34 to rotate on the inner wall of the inner enclosing plate 33, the winding hose 32 is extruded in a creeping manner, so that the medicine flows from the liquid storage box 5 to the contact spray nozzle 37 on one side of the conduit 36, the second motor 17 drives the rotary disc 39 to rotate, the notch 38 on the rotary disc 39 rotates along with acceleration, when the notch 38 is contacted with the medicine liquid flow on one side of the contact spray nozzle 37, a centrifugal atomization effect is generated under the action of centrifugal motion, the fan 7 blows the dispersing fan blades 40 in the atomization box 6 through the connecting pipe 15, the generated atomized medicine enters the blockage box, and the opening and closing of the two outlets 23 of the blockage box can be adjusted through the knob 20;

s3: then, after the liquid mist enters the anti-liquefaction component 14 from the outlet 23, under the effect of the rapid flow gasket 25, the rotating fan blades 26 are accelerated to rotate, the large liquid drops converged into particles are broken up again and sprayed out from the spray hole 27, and the sprayed atomized medicine enters the atomizing nozzle 11 through the connecting hose 13;

s4: finally, the atomized air flow enters the double-layer structure from the gap at one side of the atomizing nozzle 11, and under the guidance of the guide fan 30, the odor emitted by the inducing particles 29 is blown out from the air holes 31 to attract the mouse to approach the atomizing nozzle 11, and the atomized medicine is actively absorbed from the atomizing nozzle 11.

The previous description is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.