阅读说明：本技术 一种害虫抑制菌菌剂及其制备方法 (Pest inhibition bacterium agent and preparation method thereof ) 是由 王涛 于 2021-04-30 设计创作，主要内容包括：本发明公开了一种害虫抑制菌菌剂及其制备方法,包括所述烟沫300-500公斤,所述氨基酸粉300-500公斤,所述雷公藤芬75-125公斤,所述解淀粉芽孢杆菌75-125公斤,所述其他混合菌肥75-125公斤,本发明通过烟沫、氨基酸粉、雷公藤芬、解淀粉芽孢杆菌和其他混合菌肥混合制成,其中所含的侧孢短芽孢杆菌、荧光假单胞菌、苏云金杆菌、白僵菌等相互作用,有效预防、抑制地下害虫、根蛆等,不仅可以广泛的对蝼蛄、蛴螬、金龟子、金针虫等地下害虫的预防效果也很好,一次施肥,确保一季少无虫,也可以改善土壤结构、促进作物生长的三重功效,是绿色无公害蔬菜的首选产品,害虫抑制菌菌剂且具有安全、高效、环保、无残留、无抗药性特点。(The invention discloses a pest-inhibiting bacterial agent and a preparation method thereof, wherein the pest-inhibiting bacterial agent comprises 500 kilograms of smoke dust 300-, the pest-inhibiting bacterial agent has the characteristics of safety, high efficiency, environmental protection, no residue and no drug resistance.)

1. A pest-inhibiting bacterial agent and a preparation method thereof are characterized in that the pest-inhibiting bacterial agent comprises the following raw materials: the smoke dust is 300-500 kg, the amino acid powder is 300-500 kg, the tripterygium wilfordii is 75-125 kg, the bacillus amyloliquefaciens is 75-125 kg, and the other mixed bacterial manure is 75-125 kg.

2. The pest-inhibiting bacterial agent and the preparation method thereof according to claim 1, wherein the first formula comprises:

raw materials Components Cigarette foam 300 Amino acid powder 300 Tripterygium wilfordii hook F 75 Bacillus amyloliquefaciens 75 Other mixed bacterial manure 75

3. The pest-inhibiting bacterial agent and the preparation method thereof according to claim 1, wherein the formula two is as follows:

raw materials Components Cigarette foam 400 Amino acid powder 400 Tripterygium wilfordii hook F 100 Bacillus amyloliquefaciens 100 Other mixed bacterial manure 100

4. The pest-inhibiting bacterial agent and the preparation method thereof according to claim 1, wherein the third formula is:

5. the pest-inhibiting bacterial agent and the preparation method thereof according to claim 1, wherein the tobacco foam is rich in protein, saccharides, lignin, cellulose, organic acid, pectin and the like, and the biological fermentation of the tobacco foam is to make the high-fertility organic fertilizer by making full use of the unique physical and chemical characteristics of the tobacco foam and by using a low production cost and a simple production process.

6. The pest-controlling bacterial agent and the preparation method thereof according to claim 1, wherein the amino acid powder is a light yellow powder, is uniform and free from caking, has the inherent smell and taste of the product, is free from foreign odor and peculiar smell, and is dissolved in water.

7. The pest-inhibiting bacterial agent of claim 1, wherein the tripterygium wilfordii is extracted from tripterygium wilfordii, tripterygium wilfordii is a plant of tripterygium genus of Celastraceae family, vine shrub, high up to 3 m, red brown with small edges, leaf-ellipse, inverted egg-ellipse, rectangular ellipse or oval, with fine saw teeth on the edges, conical parasol; white in color, the tip of sepal is sharp; the petals are in the shape of rectangular ovate, the flower column is in the shape of a column, the samara is in the shape of a long circle, the small fruit stalks are in the shape of a thin circle, and the seeds are in the shape of a thin column.

8. The pest-inhibiting bacterial agent and the preparation method thereof according to claim 1, wherein the bacillus amyloliquefaciens is bacillus, is a bacterium with high affinity with bacillus subtilis, and can produce a series of metabolites capable of inhibiting the activities of fungi and bacteria in the growth process.

9. The pest-inhibiting bacterial agent and the preparation method thereof according to claim 1, wherein the other complex flora comprises brevibacillus laterosporus, pseudomonas fluorescens, bacillus thuringiensis and beauveria bassiana.

10. The method for preparing a pest-inhibiting bacterial agent according to claim 1, wherein:

step one, preparation of tobacco foam: picking, cleaning, drying and the like the tobacco leaves, and grinding the dried tobacco leaves;

step two, preparation of amino acid powder:

(1) putting the hydrolyzed protein and acid into a reaction kettle according to a proportion, heating and stirring to control reaction to obtain a hydrolysis reaction solution;

(2) neutralizing the hydrolysis reaction solution with acid liquor, and filtering and removing residues to obtain filtrate;

(3) concentrating the solution which is subjected to precision filtration and enters a concentration pot until the solution is dried to obtain a finished product;

step three, preparing a product: weighing quantitative tobacco powder, amino acid powder, tripterygium wilfordii, bacillus amyloliquefaciens and other mixed bacterial manure, sequentially adding the weighed tobacco powder, amino acid powder, tripterygium wilfordii, bacillus amyloliquefaciens and other mixed bacterial manure into a mixing stirrer to be stirred and mixed, stirring at normal temperature for 2 hours, and standing;

step four, subpackaging products: after the liquid medicine is mixed, stood and precipitated, the upper layer liquid is extracted and quantitatively added in a bottle, and then the mixture can be sealed and stored.

Technical Field

The invention relates to the field of pest-inhibiting bacterial agents, in particular to a pest-inhibiting bacterial agent and a preparation method thereof.

Background

Pests, as the name implies, i.e., harmful insects, are defined by humans as animals of some species, and are largely divided into insects and non-insects, as opposed to beneficial insects, and are diverse in species and quantity. Pests such as mosquitoes, flies, fleas, cockroaches, etc., which are frequently present in life; these small organisms often have negative effects on human life and production, and even cause death and serious disasters to plants.

In the prior art, the pest-inhibiting bacterial agents generally act on the surface of plants, so that pests attached to the surface of the plants are removed, and pests and the like below the roots of the plants are difficult to influence, so that the pest-inhibiting bacterial agents are not obvious in pest killing effect or damage plant bodies, and therefore the pest-inhibiting bacterial agents are needed.

Disclosure of Invention

The invention aims to provide a pest-inhibiting bacterial agent and a preparation method thereof, which aim to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a pest-inhibiting bacterial agent and a preparation method thereof are disclosed, the raw material selection and the components are as follows: the smoke dust is 300-500 kg, the amino acid powder is 300-500 kg, the tripterygium wilfordii is 75-125 kg, the bacillus amyloliquefaciens is 75-125 kg, and the other mixed bacterial manure is 75-125 kg.

Preferably, the first formula of the pest-inhibiting bacterial agent is as follows:

raw materials	Components
		Cigarette foam	300
Amino acid powder	300
		Tripterygium wilfordii hook F	75
Bacillus amyloliquefaciens	75
		Other mixed bacterial manure	75

Preferably, the formula of the pest-inhibiting bacterial agent is as follows:

raw materials	Components
		Cigarette foam	400
Amino acid powder	400
		Tripterygium wilfordii hook F	100
Bacillus amyloliquefaciens	100
		Other mixed bacterial manure	100

Preferably, the formula of the pest-inhibiting bacterial agent is as follows:

preferably, the tobacco foam is rich in protein, saccharides, lignin, cellulose, organic acid, pectin and the like, and the biological fermentation of the tobacco foam is used for preparing the biological organic fertilizer by fully utilizing the unique physical and chemical characteristics of the tobacco foam and preparing the organic fertilizer with high fertility by using lower production cost and simple production process.

Preferably, the amino acid powder is light yellow powder, is uniform and free of agglomeration, has the inherent smell and taste of the product, is free of peculiar smell and peculiar smell, and is dissolved in water.

Preferably, the tripterygium wilfordii is extracted from tripterygium wilfordii, which is a plant of tripterygium genus of Celastraceae family, is a vine shrub, and has a height of up to 3 m, a small branch, a thin edge, a brownish red color, an elliptical leaf shape, an inverted oval shape, a rectangular elliptical shape or an oval shape, and fine sawteeth and a conical parasol inflorescence at the edge; white in color, the tip of sepal is sharp; the petals are in the shape of rectangular ovate, the flower column is in the shape of a column, the samara is in the shape of a long circle, the small fruit stalks are in the shape of a thin circle, and the seeds are in the shape of a thin column.

Preferably, the bacillus amyloliquefaciens is bacillus, is a bacterium with high affinity with bacillus subtilis, and can produce a series of metabolites capable of inhibiting the activities of fungi and bacteria in the growth process.

Preferably, the other complex flora comprises brevibacillus laterosporus, pseudomonas fluorescens, bacillus thuringiensis and beauveria bassiana.

Preferably, a preparation method of the pest-inhibiting bacterial agent,

step one, preparation of tobacco foam: picking, cleaning, drying and the like the tobacco leaves, and grinding the dried tobacco leaves;

step two, preparation of amino acid powder:

(1) putting the hydrolyzed protein and acid into a reaction kettle according to a proportion, heating and stirring to control reaction to obtain a hydrolysis reaction solution;

(2) neutralizing the hydrolysis reaction solution with acid liquor, and filtering and removing residues to obtain filtrate;

(3) concentrating the solution which is subjected to precision filtration and enters a concentration pot until the solution is dried to obtain a finished product;

step three, preparing a product: weighing quantitative tobacco powder, amino acid powder, tripterygium wilfordii, bacillus amyloliquefaciens and other mixed bacterial manure, sequentially adding the weighed tobacco powder, amino acid powder, tripterygium wilfordii, bacillus amyloliquefaciens and other mixed bacterial manure into a mixing stirrer to be stirred and mixed, stirring at normal temperature for 2 hours, and standing;

step four, subpackaging products: after the liquid medicine is mixed, stood and precipitated, the upper layer liquid is extracted and quantitatively added in a bottle, and then the mixture can be sealed and stored.

The invention has the technical effects and advantages that: the pest-inhibiting bacterial agent is prepared by mixing tobacco powder, amino acid powder, tripterygium wilfordii, bacillus amyloliquefaciens and other mixed bacterial fertilizers, wherein the contained brevibacillus laterosporus, pseudomonas fluorescens, bacillus thuringiensis, muscardine and the like interact with each other, so that underground pests, root maggots and the like are effectively prevented and inhibited, organic planting requirements are met, the pest-inhibiting bacterial agent not only can be widely good in prevention effect on underground pests such as mole crickets, grubs, chafers, wireworms and wireworms, but also can be used for improving the soil structure, preventing and controlling soil-borne diseases and promoting the growth of crops after being fertilized once, is a preferred product of green pollution-free vegetables, and has the characteristics of safety, high efficiency, environmental protection, no residue and no drug resistance.

Detailed Description

The technical scheme of the invention is as follows: the invention provides a pest-inhibiting bacterial agent and a preparation method thereof, and the pest-inhibiting bacterial agent comprises the following raw materials in percentage by weight: 500 kg of smoke dust, 500 kg of amino acid powder, 75-125 kg of tripterygium wilfordii, 75-125 kg of bacillus amyloliquefaciens and 75-125 kg of other mixed bacterial manure.

Further, the first formula of the pest-inhibiting bacterial agent is as follows:

raw materials	Components
		Cigarette foam	300
Amino acid powder	300
		Tripterygium wilfordii hook F	75
Bacillus amyloliquefaciens	75
		Other mixed bacterial manure	75

Further, the first formula of the pest-inhibiting bacterial agent is as follows: the second formula is as follows:

raw materials	Components
		Cigarette foam	400
Amino acid powder	400
		Tripterygium wilfordii hook F	100
Bacillus amyloliquefaciens	100
		Other mixed bacterial manure	100

Further, the first formula of the pest-inhibiting bacterial agent is as follows: the third formula is as follows:

raw materials	Components
		Cigarette foam	450
Amino acid powder	450
		Tripterygium wilfordii hook F	125
Bacillus amyloliquefaciens	125
		Other mixed bacterial manure	125

Furthermore, the tobacco foam is rich in protein, carbohydrate, lignin, cellulose, organic acid, pectin and the like, and the biological fermentation of the tobacco foam is used for preparing the biological organic fertilizer by fully utilizing the unique physical and chemical characteristics of the tobacco foam and preparing the organic fertilizer with high fertility by using lower production cost and simple production process.

Further, the amino acid powder is a light yellow powder, is uniform and free from caking, has the inherent smell and taste of the product, is free from foreign odor and peculiar smell, and is dissolved in water.

Further, tripterygium wilfordii is extracted from tripterygium wilfordii, which is a plant of tripterygium genus of Celastraceae family, is liana shrub, and has a height of up to 3 m, a small branch, a thin edge, a brownish red color, an elliptical leaf, an inverted oval, a rectangular ellipse or an oval shape, and fine saw teeth and a conical polypodium on the edge; white in color, the tip of sepal is sharp; the petals are in the shape of rectangular ovate, the flower column is in the shape of a column, the samara is in the shape of a long circle, the small fruit stalks are in the shape of a thin circle, and the seeds are in the shape of a thin column.

Further, bacillus amyloliquefaciens is a bacillus genus, a bacterium with high affinity to bacillus subtilis, and can produce a series of metabolites capable of inhibiting the activity of fungi and bacteria during the growth process.

Further, other complex bacteria include Brevibacillus laterosporus, Pseudomonas fluorescens, Bacillus thuringiensis, Beauveria bassiana.

Further, a method for preparing a pest-inhibiting bacterial agent,

step one, preparation of tobacco foam: picking, cleaning, drying and the like the tobacco leaves, and grinding the dried tobacco leaves;

step two, preparation of amino acid powder:

(1) putting the hydrolyzed protein and acid into a reaction kettle according to a proportion, heating and stirring to control reaction to obtain a hydrolysis reaction solution;

(2) neutralizing the hydrolysis reaction solution with acid liquor, and filtering and removing residues to obtain filtrate;

(3) concentrating the solution which is subjected to precision filtration and enters a concentration pot until the solution is dried to obtain a finished product;

step three, preparing a product: weighing quantitative tobacco powder, amino acid powder, tripterygium wilfordii, bacillus amyloliquefaciens and other mixed bacterial manure, sequentially adding the weighed tobacco powder, amino acid powder, tripterygium wilfordii, bacillus amyloliquefaciens and other mixed bacterial manure into a mixing stirrer to be stirred and mixed, stirring at normal temperature for 2 hours, and standing;

step four, subpackaging products: after the liquid medicine is mixed, stood and precipitated, the upper layer liquid is extracted and quantitatively added in a bottle, and then the mixture can be sealed and stored.

The working principle of the invention is as follows: the pest-inhibiting microbial inoculum adopts a specific microbial inoculum developed for Chinese chive maggots, onion maggots, underground insects and the like according to the life activity characteristics of underground common pests, and the effective components of the product are as follows: the complex flora (Brevibacillus laterosporus, Pseudomonas fluorescens, Bacillus thuringiensis and Beauveria bassiana) is more than or equal to 50 hundred million/g. The pseudomonas fluorescens in the product can generate novel insect-resistant protein, and can effectively control various underground pests; bacillus thuringiensis is a bacterial insecticide, and after pests eat the insecticide, septicemia is generated in the digestive tract to die; beauveria bassiana is a fungal pesticide, after application, spores germinate under appropriate conditions by directly contacting with the insect body or being eaten into the alimentary canal by pests on crops, and propagate in the insect body to consume nutrients of the insect body and produce beauvericin, oospore beauvericin and the like, so that pests die slowly. The product does not cause rapid dehydration of insects, so the lethal effect is slow, when the product is used for preventing and treating larvae, the product can be sprayed by using 200-fold liquid of 400 times of the product to irrigate roots or a high-pressure sprayer along ridges, the spraying is carried out once again at intervals of 15 days, when the imagoes are prevented and treated, the powder is spread along the ridges in the full growth period of the imagoes, 1-2 kg of the powder is uniformly mixed with 10 times of dry fine soil per mu, and then the mixture is spread around the roots of crops, and then the watering is carried out. Or the microbial inoculum is diluted by 200 times and 400 times, the stem leaves are sprayed, and the soil surface around the crops is also sprayed. Particularly, in autumn, the imagoes are concentrated and seriously damaged, the prevention and the treatment are emphasized, the required attention is that the imagoes cannot be mixed with alkaline pesticides or bactericides, the application effect is best when the imagoes are applied to the low-age larvae, the imagoes are stored in shady and dry places to prevent the imagoes from being affected with damp, the bacterium solution is used up within 2 hours after being prepared, so that the spore germination is prevented from losing the capability of infecting the insects, the germination of the spore of the fungicide requires certain humidity, therefore, the application is carried out at noon of high temperature and strong light, the insecticide is preferably applied when the humidity is high in cloudy days, rainy days or in the early and late days, and the imagoes are forbidden to be used in silkworm breeding areas.

The standard parts used by the invention can be purchased from the market, and the special-shaped parts can be customized according to the description of the specification.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.