阅读说明：本技术 一种防治甘蔗蓟马的杀虫剂 (Pesticide for preventing and treating sugarcane thrips ) 是由 邓宇驰 周忠凤 罗霆 翁梦苓 王宇萍 李傲梅 陈荣发 周慧文 王伦旺 吴建明 于 2021-08-19 设计创作，主要内容包括：本发明涉及农药技术领域,具体涉及一种防治甘蔗蓟马的杀虫剂。一种防治甘蔗蓟马的杀虫组合物,其活性成分由藻酸丙二醇酯与乙基多杀菌素或环氧虫啉二元复配而成；其中,所述藻酸丙二醇酯和乙基多杀菌素的质量比为1-30：15-1；所述藻酸丙二醇酯和环氧虫啉的质量比为1-20：20-1。本发明的两种活性成分复配时无交互抗性,在一定质量比范围内表现出增效作用,与单剂相比可以提高对甘蔗蓟马的防治效果,这样可以减少农药的喷施剂量,降低农药残留；其次本发明两种活性成分杀灭甘蔗蓟马的作用机理不同,这样可以延缓害虫抗药性的产生,对药剂本身具有一定的保护作用。(The invention relates to the technical field of pesticides, and particularly relates to an insecticide for preventing and treating sugarcane thrips. An insecticidal composition for preventing and treating sugarcane thrips, which is prepared by binary compounding of propylene glycol alginate and spinetoram or epoxy imidacloprid as active ingredients; wherein the mass ratio of the propylene glycol alginate to the spinetoram is 1-30: 15-1; the mass ratio of the propylene glycol alginate to the epoxy imidacloprid is 1-20: 20-1. The two active ingredients of the invention have no cross resistance when being compounded, show synergistic action in a certain mass ratio range, and can improve the control effect on sugarcane thrips compared with a single agent, thereby reducing the spraying dosage of pesticide and reducing pesticide residue; secondly, the two active ingredients of the invention have different action mechanisms for killing the sugarcane thrips, thus being capable of delaying the generation of drug resistance of pests and having certain protection effect on the pesticide.)

1. The insecticidal composition for preventing and treating sugarcane thrips is characterized in that the active ingredients are formed by binary combination of propylene glycol alginate and spinetoram or epoxy imidacloprid; wherein the mass ratio of the propylene glycol alginate to the spinetoram is 1-30: 15-1; the mass ratio of the propylene glycol alginate to the epoxy imidacloprid is 1-20: 20-1.

2. The insecticidal composition according to claim 1, wherein the propylene glycol alginate and spinetoram are in a mass ratio of 1: 5.

3. the insecticidal composition according to claim 1, wherein the propylene glycol alginate and the epoxycholine are present in a mass ratio of 3: 1.

4. a pesticide containing the insecticidal composition of claim 1, wherein the mass percentage of the active ingredient to the total mass of the pesticide is 1-80%, and the balance is an agriculturally and pharmaceutically acceptable auxiliary.

5. The insecticide according to claim 4, wherein the mass percentage of the active ingredient to the total mass of the insecticide is 15-60%.

6. The insecticide according to claim 4, wherein the formulation of the insecticide is any formulation allowed to be used in agriculture, and comprises wettable powder, water dispersible granules, suspending agents and emulsion in water.

7. The insecticide according to claim 4, wherein the agriculturally and pharmaceutically acceptable auxiliary agents comprise one or more of fillers, wetting agents, emulsifiers, binders, disintegrants, dispersants, thickeners, preservatives, antifoaming agents, anti-freezing agents, solvents and stabilizers.

8. Use of the insecticidal composition according to any one of claims 1 to 3 or the insecticide according to any one of claims 4 to 7 for the control of sugarcane thrips.

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to an insecticide for preventing and treating sugarcane thrips.

Background

Sugarcane (Saccharum officinarum L.) belongs to annual perennial root tropical and subtropical herbaceous plants, belongs to C4 crops, is a main sugar crop in China, has the sugar yield accounting for more than 90 percent of the total sugar yield in China, and has important positions and functions in agricultural economic income. Sugarcane pests are various in variety, and the sugarcane is threatened by different insect pests in different growth periods, so that the sugarcane yield and quality are greatly influenced. In recent years, with the adjustment of industrial structures, the sugarcane planting area is enlarged, the planting mode is changed, the climate in sugarcane areas is abnormal, various pests such as sugarcane diseases, cordyceps sinensis and the like tend to rise year by year, and serious loss is caused to the production of sugarcane. The potential loss rate of sugarcane production caused by pests reaches 15% -30%. The major growth period of the sugarcane in 7-8 months is the most critical period of high-yield and high-sugar cultivation of the sugarcane, the peak period of mixed outbreak of various pests such as sugarcane woolly aphids, thrips, 3 rd and 4 th generation sugarcane borers and the like is also the peak period, the elongation of the sugarcane joints is often prevented by the harm of various pests, the yield is reduced, the sugar is reduced, and the scientific and effective prevention and control of the pests at the time is the fundamental guarantee for realizing the aim of double-high (high-yield and high-sugar) sugarcane.

Sugarcane thrips (Balothrips serratus Kobus), also known as sugarcane brown thrips, belongs to Thysanoptera and thrips, is one of important pests harmful to sugarcane, and is widely distributed in sugarcane-planting areas in China. Thrips are hosted in sugarcane, the thrips of the sugarcane are small in size, hidden in life (mainly concentrated in new leaves of the sugarcane leaves), and high in breeding speed, and the thrips are mainly harmful to the new leaves of the sugarcane and the tails of the sugarcane leaves; adult and nymphs of sugarcane thrips are hidden in sugarcane heart leaves and file and suck juice in the leaves, the damaged leaves are slightly in water stain-like yellow spots when not unfolded, and the leaves are yellow or light yellow patches when unfolded. After thrips are harmful, the damaged leaves are yellow-white and chlorosis scars, the leaves become yellow brown when serious, the leaf tips are curled and dried, even a plurality of leaves at the top end are rolled together and can not be unfolded, the plants are short and yellow, and the yield of the sugarcane is influenced; especially when the sugarcane thrips explode, the sugarcane leaves are coiled and withered, so that the sugarcane leaves are wounded and knotted to cause tip rot, even die due to withering, influence on photosynthesis of leaves, seriously hinder the growth of the sugarcane and cause yield reduction and sugar content reduction; when the damage is serious, the plant damage rate can reach 100 percent, and the sugarcane yield and the planting benefit of farmers are greatly influenced. The generation is more than 10 generations each year, the generation begins after spring-warming every year, the full-fleshed period is entered in 5-6 months, and the insect population is sharply reduced after autumn. The insect can complete 1 generation in more than 10 days, and the generation is overlapped, and the egg is laid in the heart leaf tissue. Adults and nymphs like drought and backlight environment and are mostly hidden in undeployed heart leaves as pests. Adults often spread with the airflow. In summer, the sugarcane grows slowly or the low-lying area of the sugarcane field is accumulated with water and seriously lacks fertilizer, and the thrips are serious.

The specific measures for preventing and treating the sugarcane thrips comprise comprehensive measures such as agricultural control, physical control, biological control, chemical control and the like. Agricultural control mainly aims at enhancing water and fertilizer management, promoting crops to grow and grow quickly, and reducing harm by reducing the living time of thrips on new leaves; the physical control is to hang blue or yellow sticky insect plates in the field to trap the thrips imagoes and reduce the population of the field insects; biological control is the control of thrips hazards by releasing or protecting predatory natural enemies; chemical control is the killing of thrips and the protection of crops by the application of chemical pesticides. At present, chemical control is mainly used for sugarcane thrips in sugarcane areas in China, most of sugarcane thrips are dispersedly controlled by households, multiple pests need to be controlled by pesticide application for many times, labor and time are wasted, labor and water are difficult to use, the control cost is high, the pests are easy to generate drug resistance, pesticide residues, the environment is polluted, natural enemies are killed, and the control difficulty is increased. In recent years, with the change of environment and the unreasonable use of pesticides, and the increase of sugarcane continuous planting and continuous cropping, thrips are getting more and more serious.

In the planting process of sugarcane, chemical spraying agents are generally adopted to kill sugarcane thrips, such as carbosulfan, imidacloprid or chlorpyrifos. However, the pesticide registered for preventing and treating sugarcane pests is less, so that the drug resistance of sugarcane thrips is easily caused by using a single chemical agent for a long time, the sugarcane thrips has certain drug resistance to the existing chemical agents, the prevention and treatment effect of the existing chemical agents on the sugarcane thrips is gradually reduced, and the defect of pesticide residue exists when the chemical agents are applied in large quantities.

Propylene glycol alginate with the molecular formula of (C)₉H₁₄O₇) n is prepared from alginic acid extracted from seaweed through esterification reaction. The seaweed propylene glycol ester is a unique insecticidal substance, has the action mode of contact killing, quickly closes or blocks the stomata for respiratory metabolism of pests by using the specific viscosity and physical contact killing property of the seaweed propylene glycol ester, and blocks the circulation path and metabolism of the pests to finally kill the pests.

Ethyl spinosad is a multi-bacteriocin pesticide, is obtained by chemically modifying natural products spinosyns, belongs to a bionic pesticide, is a brand-new, unique and efficient broad-spectrum pesticide, acts on a nicotinic acetylcholine receptor and an r-aminobutyric acid receptor in insect nerves, causes the insect bodies to be insensitive to excitatory or inhibitory signal transmission reaction, influences normal nervous activity until death, and can effectively prevent and control pests such as diamond back moths, beet armyworms, thrips, prodenia litura and the like.

The epoxy imidacloprid is a neonicotinoid insecticide, has the function of selectively inhibiting a nicotinic acetylcholinesterase receptor of an insect nervous system, and is a low-toxicity and high-efficiency insecticide with wide insecticidal spectrum and novel structure.

Compounding pesticides with different action mechanisms is a convenient method for researching and developing novel pesticides, and three action types can appear after compounding: antagonism, addition and synergism, wherein the combination of synergistic action types can improve the control effect and reduce the application amount of pesticide, thereby reducing the pesticide residue. The inventor finds that when propylene glycol alginate and spinetoram or epoxy imidacloprid are subjected to binary compounding according to a certain mass ratio through a large number of indoor biological activity tests, the obvious synergistic effect is shown. No report on this aspect has been found so far.

The above background disclosure is only for the purpose of assisting understanding of the inventive concept and technical solutions of the present invention, and does not necessarily belong to the prior art of the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.

Disclosure of Invention

The invention aims to provide a pesticide for preventing and treating sugarcane thrips, which aims to solve the problem that the prevention and treatment effect of the existing chemical agents on the sugarcane thrips is gradually reduced.

In order to achieve the purpose, the invention provides the following technical scheme:

an insecticidal composition for preventing and treating sugarcane thrips, which is prepared by binary compounding of propylene glycol alginate and spinetoram or epoxy imidacloprid as active ingredients; wherein the mass ratio of the propylene glycol alginate to the spinetoram is 1-30: 15-1; the mass ratio of the propylene glycol alginate to the epoxy imidacloprid is 1-20: 20-1.

According to the technical scheme, the mass ratio of the propylene glycol alginate to the spinetoram is 1: 5.

according to the technical scheme, the mass ratio of the propylene glycol alginate to the epoxy worm-like amine is 3: 1.

the second object of the present invention: the pesticide containing the pesticide composition is provided, the mass of the active ingredient accounts for 1-75% of the total mass of the pesticide, and the balance is an agriculturally and pharmaceutically acceptable auxiliary agent.

As a further arrangement of the technical scheme of the invention, the mass percentage of the active ingredients in the total mass of the pesticide is 15-60%.

As a further arrangement of the technical scheme, the pesticide is any agriculturally acceptable dosage form, including wettable powder, water dispersible granules, a suspending agent and an emulsion in water.

As a further arrangement of the technical scheme of the invention, the auxiliary agent acceptable in the agricultural pharmacy consists of one or more of filler, wetting agent, emulsifier, binder, disintegrant, dispersant, thickener, preservative, defoaming agent, antifreezing agent, solvent and stabilizer;

for wettable powders, the auxiliaries which can be used are: a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; the filler is selected from one or more of ammonium sulfate, urea, sucrose, glucose, diatomaceous earth, attapulgite, light calcium carbonate, pulvis Talci, white carbon black, pottery clay, and kaolin.

For water dispersible granules, the auxiliaries that can be used are: a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; disintegrating agent such as one or more of sodium sulfate, urea, sucrose, and glucose; binder such as one or more of diatomaceous earth, corn starch, carboxymethyl cellulose; the filler is one or more of diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder and attapulgite.

For the suspending agents, usable are: dispersing agents such as one or more of polycarboxylate, lignosulfonate; emulsifier such as one or more of Nongru 700#, Nongru 2201, sorbitan stearate, Tween-60 #, and Nongru 1601 #; wetting agents such as one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; thickening agent such as one or more of xanthan gum, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; preservatives such as one or more of formaldehyde, benzoic acid, sodium benzoate; defoaming agents such as silicone defoaming agents; antifreeze agents such as one or more of ethylene glycol, propylene glycol, glycerol, urea, and sodium chloride.

For aqueous emulsions, auxiliaries which can be used are: the emulsifier is one or more of agricultural milk 600# phosphate, agricultural milk 700#, agricultural milk 2201#, Span-60#, emulsifier T-60, TX-10, agricultural milk 1601, agricultural milk 400#, and agricultural milk 600 #; solvents such as one or more of xylene and toluene; one or more of stabilizers such as triphenyl phosphite, epichlorohydrin and acetic anhydride; antifreeze agents such as one or more of ethylene glycol, propylene glycol, glycerol, urea, and sodium chloride; thickening agent such as one or more of xanthan gum, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; preservatives such as one or more of formaldehyde, benzoic acid, sodium benzoate.

A third object of the invention: provides the application of the insecticidal composition or the insecticide in preventing and treating sugarcane thrips.

Compared with the prior art, the invention has the following beneficial effects:

the two active ingredients of the invention have no cross resistance when being compounded, show synergistic effect within a certain mass ratio range, and can improve the control effect on sugarcane thrips compared with a single agent, thereby reducing the spraying dosage of pesticide and reducing pesticide residue; secondly, the two active ingredients of the invention have different action mechanisms for killing the sugarcane thrips, thus being capable of delaying the generation of drug resistance of pests and having certain protection effect on the pesticide.

Detailed Description

The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.

Example 1: indoor bioactivity test of propylene glycol alginate complex

Test subjects: sugarcane thrips collected from sugarcane planting fields were raised in a laboratory, and 2-year-old nymphs were selected as test subjects.

Reagent to be tested: 98% of propylene glycol alginate raw material, 81.2% of spinetoram raw material and 95% of epoxy imidacloprid raw material.

The test method comprises the following steps: (refer to "NY/T1154.6-2006 indoor bioassay Standard for agricultural chemicals insecticide part 6: insect-soaking method for insecticidal Activity test")

1. Dissolving the original medicine with acetone, diluting with 0.1% Tween-80 water solution to respectively prepare single-dose mother liquor, setting multiple groups of ratios, setting 6 gradient mass concentrations for each single dose and each group of ratio mixture, and 50mL of each mass concentration liquid medicine;

2. soaking the second-instar nymphs of sugarcane thrips into the medicinal liquid for 10s, sucking the redundant medicinal liquid by using filter paper, transferring the medicinal liquid to the breeding under normal conditions, repeating the treatment for 4 times, soaking 20 insects for each time, and setting a solution only containing acetone and Tween-80 as a blank control;

3. observing the death condition of sugarcane thrips 24h after treatment, calculating the corrected death rate of each treatment, performing regression analysis on the concentration log values and the corresponding corrected death rate probability values of each single agent and the mixed agents with different proportions by using DPS software, and calculating EC₅₀And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.

P₁(K ÷ N) × 100, where P₁: mortality rate; k: the number of dead insects; n: treating the total number of insects;

P₂＝[(P_t-P₀)÷(1-P₀)]x 100, wherein, P₂: correcting mortality; p_t: (ii) managing mortality; p₀: blank control mortality;

measured virulence index (ATI) ═ standard agent EC₅₀Reagent for test EC₅₀)×100；

Theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture;

co-toxicity coefficient (CTC) × 100 [ actually measured toxicity index (ATI) ÷ theoretical toxicity index of the mixture (TTI) ].

Criteria are divided according to joint action: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect. The results are shown in tables 1-2.

TABLE 1 indoor bioactivity assay of propylene glycol alginate and spinetoram combinations on sugarcane thrips

Name and proportion of the medicament	EC50(mg/L)	ATI	TTI	CTC
					Propylene glycol alginate	0.79	100.00	--	--
Spinetoram	0.63	125.40	--	--
					Propylene glycol alginate 1: spinetoram 15	0.28	282.14	123.81	227.88
Propylene glycol alginate 1: spinetoram 12	0.41	192.68	123.44	156.09
					Propylene glycol alginate 1: spinetoram 9	0.53	149.06	122.86	121.33
Propylene glycol alginate 1: spinetoram 7	0.31	254.84	122.22	208.50
					Propylene glycol alginate 1: spinetoram 5	0.19	415.79	121.16	343.16
Propylene glycol alginate 1: spinetoram 3	0.35	225.71	119.05	189.60
					Propylene glycol alginate 1: spinetoram 1	0.44	179.55	112.70	159.31
Propylene glycol alginate 5: spinetoram 1	0.56	141.07	104.23	135.34
					Propylene glycol alginate 10: spinetoram 1	0.39	202.56	102.31	197.99
Propylene glycol alginate 15: spinetoram 1	0.24	329.17	101.59	324.02
					Propylene glycol alginate 20: spinetoram 1	0.18	438.89	101.21	433.64
Propylene glycol alginate 25: spinetoram 1	0.62	127.42	100.98	126.19
					Propylene glycol alginate 30: spinetoram 1	0.46	171.74	100.82	170.34

As can be seen from Table 1, propylene glycol alginate and spinetoram are mixed in a mass ratio of 1-30: the cotoxicity coefficients within the range of 15-1 are all larger than 120, namely the compounded propylene glycol alginate and spinetoram have synergistic effect on the biological activity of sugarcane thrips.

TABLE 2 indoor bioactivity assay of compounded propylene glycol alginate and oximinostrobin on sugarcane thrips

Name and proportion of the medicament	EC50(mg/L)	ATI	TTI	CTC
					Propylene glycol alginate	0.79	100.00	--	--
Epoxy imidacloprid	7.07	11.17	--	--
					Propylene glycol alginate 1: epoxy imidacloprid 20	2.74	28.83	15.40	187.18
Propylene glycol alginate 1: epoxy imidacloprid 15	1.88	42.02	16.73	251.24
					Propylene glycol alginate 1: epoxy imidacloprid 10	1.42	55.63	19.25	289.02
Propylene glycol alginate 1: epoxy imidacloprid 5	0.82	96.34	25.98	370.85
					Propylene glycol alginate 1: epoxy imidacloprid 3	1.01	78.22	33.38	234.32
Propylene glycol alginate 1: epoxy imidacloprid 1	1.02	77.45	55.59	139.33
					Propylene glycol alginate 3: epoxy imidacloprid 1	0.18	438.89	77.79	564.17
Propylene glycol alginate 5: epoxy imidacloprid 1	0.32	246.88	85.20	289.77
					Propylene glycol alginate 10: epoxy imidacloprid 1	0.47	168.09	91.92	182.85
Propylene glycol alginate 15: epoxy imidacloprid 1	0.52	151.92	94.45	160.85
					Propylene glycol alginate 20: epoxy imidacloprid 1	0.29	272.41	95.77	284.45

As can be seen from Table 2, the ratio of propylene glycol alginate to epoxychonglin by mass is 1-20: the co-toxicity coefficients within the range of 20-1 are all larger than 120, namely the compounded propylene glycol alginate and the epoxy imidacloprid have synergistic effect on the biological activity of the sugarcane thrips.

In conclusion, when the propylene glycol alginate and the spinetoram or the epoxy imidacloprid are subjected to binary compounding according to a certain mass ratio, the co-toxicity coefficients are all larger than 120, the synergistic effect is shown, and compared with a single agent, the control effect on the sugarcane thrips can be improved.

It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.