阅读说明：本技术 一种便携式全自动微流控装置及其检测淡水水质的方法 (Portable full-automatic microfluidic device and method for detecting fresh water quality by using same ) 是由 尹彬沣 蓝良锐 周佟 于 2021-09-27 设计创作，主要内容包括：一种便携式全自动微流控装置及其检测淡水水质的方法。本发明公开了一种便携式全自动微流控装置及其检测方法,包括集成组件、反应组件和检测组件,集成组件包括集成箱,反应组件和检测组件均设置在集成箱内,反应组件包括电机支架和容纳盒,容纳盒内可拆卸地连接有检测卡,检测卡包括检测盖板,检测盖板内连接有连接在一起的检测芯片和芯片盖板,检测盖板上侧连接有检测外壳,检测芯片上排布有若干存储池,芯片盖板上排布有若干与存储池一一对应的进液进气孔,电机支架上连接有位置可调的第一气管板,第一气管板上排布有若干气体注射阀门,气体注射阀门的内腔能与对应的进液进气孔连通；检测组件包括连接在电机支架上的检测器、位置可调的发光支架和相机支架,相机支架上连接有光感应镜头；本发明检测方便。(A portable full-automatic micro-fluidic device and a method for detecting the quality of fresh water. The invention discloses a portable full-automatic microfluidic device and a detection method thereof, and the device comprises an integrated assembly, a reaction assembly and a detection assembly, wherein the integrated assembly comprises an integrated box, the reaction assembly and the detection assembly are both arranged in the integrated box, the reaction assembly comprises a motor support and an accommodating box, a detection card is detachably connected in the accommodating box, the detection card comprises a detection cover plate, a detection chip and a chip cover plate which are connected together are connected in the detection cover plate, the upper side of the detection cover plate is connected with a detection shell, a plurality of storage pools are distributed on the detection chip, a plurality of liquid inlet holes which are in one-to-one correspondence with the storage pools are distributed on the chip cover plate, a first air pipe plate with an adjustable position is connected on the motor support, a plurality of gas injection valves are distributed on the first air pipe plate, and the inner cavities of the gas injection valves can be communicated with the corresponding liquid inlet holes; the detection assembly comprises a detector connected to the motor support, a position-adjustable light-emitting support and a camera support, and the camera support is connected with a light-sensing lens; the invention is convenient to detect.)

1. A portable full-automatic microfluidic device is characterized in that: which comprises the steps of preparing a mixture of a plurality of raw materials,

the integrated assembly (100) comprises an integrated box (103) with an upward opening, and an openable box cover (102) is connected to the upper side of the integrated box (103);

the reaction assembly (300) is arranged in the integrated box (103), the reaction assembly (300) comprises a motor support (315) and an accommodating box (302), a detection card (301) is detachably connected in the accommodating box (302), the detection card (301) comprises a detection cover plate (301d), a mounting groove (301d-1) is formed in the detection cover plate (301d), a detection chip (301c) is connected in the mounting groove (301d-1) of the detection cover plate (301d), a chip cover plate (301b) is connected to the upper side of the detection chip (301c), a detection shell (301a) is connected to the upper side of the detection cover plate (301d), a plurality of storage pools (301c-9) are distributed on the upper portion of the detection chip (301c), a plurality of liquid inlet holes (301b-1) which are in one-to-one correspondence with the storage pools (301c-9) are distributed on the chip cover plate (301b), a plurality of connecting holes (301a-1) which are in one-to-one correspondence with the liquid inlet holes (301b-1) are distributed in the detection shell (301a), a first air tube plate (308) which is adjustable in position and is arranged opposite to one end of the containing box (302) is connected to the motor support (315), a plurality of gas injection valves (309) which are in one-to-one correspondence with the connecting holes (301a-1) are distributed in the first air tube plate (308), and when the gas injection valves (309) are inserted into the corresponding connecting holes (301a-1), inner cavities of the gas injection valves (309) are communicated with the corresponding liquid inlet holes (301 b-1);

detection subassembly (200), detection subassembly (200) set up in collection box (103), and detection subassembly (200) are including connecting detector (201), position adjustable light emitting support (203) and camera support (204) on motor support (315), detector (201) holds box (302) other end setting relatively, be connected with luminous lamp tube (214) on light emitting support (203), luminous lamp tube (214) can just set up detector (201), be connected with the photoinduction lens on camera support (204).

2. The portable fully automated microfluidic device according to claim 1 wherein: the motor supports (315) on the two ends of the accommodating box (302) in the outward direction are fixedly connected with at least one fixed column (316) and a first linear driver (306), the fixed column (316) penetrates through a first air pipe plate (308) and then is fixedly connected to a second air pipe plate (307), the first air pipe plate (308) is arranged between the second air pipe plate (307) and the accommodating box (302), the first linear driver (306) is connected with a push-pull rod (305) capable of performing reciprocating horizontal linear movement, and one end, extending outwards, of the push-pull rod (305) is connected with the first air pipe plate (308).

3. The portable fully automated microfluidic device according to claim 1 wherein: reaction unit (300) is still including fixing air pump (314) in collection box (103), be connected with gaseous output base (320) on air pump (314), the venthole (320a) that the same with feed liquor inlet port (301b-1) quantity of having arranged on gaseous output base (320), venthole (320a) department of gaseous output base (320) is connected with pipeline (310), and the end connection of gaseous injection valve (309) has injection portion (309a), the one end that gaseous output base (320) were kept away from in pipeline (310) is connected with corresponding gaseous injection valve (309).

4. The portable fully automatic microfluidic device according to any one of claims 1 to 3 wherein: it has first installation cavity to open on luminous support (203), fixedly connected with fluorescent tube support (213) in the first installation cavity of luminous support (203), fluorescent tube support (213) in-connection has luminous fluorescent tube (214), is equipped with light-emitting mouth (213a) on fluorescent tube support (213), rotationally is connected with sleeve pipe (209) on luminous support (203), fluorescent tube support (213) are in sleeve pipe (209), a plurality of anti-dazzling screens (211) have been arranged in sleeve pipe (209) outside.

5. The portable fully automatic microfluidic device according to any one of claims 1 to 3 wherein: a plurality of first liquid outlet channels (301c-10) which are in one-to-one correspondence with the storage pool (301c-9) are distributed on the detection chip (301c), a shunt valve groove is arranged on the detection chip (301c) at one end, far away from the storage pool (301c-9), of the first liquid outlet channel (301c-10), a second liquid outlet channel (301c-7) is arranged on the detection chip (301c) at one end, far away from the storage pool (301c-9), of the shunt valve groove, a reaction pool (301c-6) is arranged on the detection chip (301c) at one end, far away from the storage pool (301c-9), of the second liquid outlet channel (301c-7), and a third liquid outlet channel (301c-1) is arranged on the detection chip (301c) at one end, far away from the second liquid outlet channel (301c-7), of the reaction pool (301c-6), a reaction valve groove (301c-2) is arranged on the detection chip (301c) at one end of the third liquid outlet channel (301c-1) far away from the reaction tank (301c-6), a fourth liquid outlet channel (301c-5) is arranged on the detection chip (301c) at one end of the reaction valve groove (301c-2) far away from the third liquid outlet channel (301c-1), a heavy metal detection tank (301c-3) and a waste liquid tank (301c-4) are arranged on the detection chip (301c) at one end of the fourth liquid outlet channel (301c-5) far away from the reaction valve groove (301c-2), and the heavy metal detection tank (301c-3) and the waste liquid tank (301c-4) can be respectively communicated with the reaction tank (301 c-6); it has first reaction feed liquor through-hole (301c-8) with reaction tank (301c-6) intercommunication to open on detecting chip (301c), it has second reaction feed liquor through-hole (301a-2) with the axle center with first reaction feed liquor through-hole (301c-8) to open on chip apron (301 b).

6. The portable fully automated microfluidic device according to claim 5 wherein: the detection chip (301c) is rotatably connected with a first micro-fluidic valve (301f) through a shunt valve slot, a first liquid outlet slot (301f-2) is formed in the first micro-fluidic valve (301f), when the first liquid outlet slot (301f-2) is communicated with any liquid outlet channel, a second liquid outlet channel (301c-7) is communicated with the corresponding first liquid outlet slot (301f-2), and when the first liquid outlet slot (301f-2) is staggered with any first liquid outlet channel (301c-10), the first liquid outlet channel (301c-10) is closed; the detection chip (301c) is rotatably connected with a second micro-fluidic valve (301e) through a reaction valve slot (301c-2), a second liquid outlet slot (301e-1) is formed in the second micro-fluidic valve (301e), the third liquid outlet channel (301c-1) is communicated with a fourth liquid outlet channel (301c-5) through the second liquid outlet slot (301e-1), when the second liquid outlet slot (301e-1) and the third or fourth liquid outlet channel (301c-5) are staggered, one end, far away from the reaction pool (301c-6), of the third liquid outlet channel (301c-1) is closed, and one end, opposite to the reaction valve slot (301c-2), of the fourth liquid outlet channel (301c-5) is also closed.

7. The portable fully automated microfluidic device according to claim 6 wherein: the upper end of the first micro-fluidic valve (301f) is provided with a first knob (301f-1), the upper end of the second micro-fluidic valve (301e) is provided with a second knob (301e-2), the chip cover plate (301b) is provided with a first mounting hole (301b-3) allowing the first micro-fluidic valve (301f) to pass through and a second mounting hole (301b-2) allowing the second micro-fluidic valve (301e) to pass through, the upper end of the first knob (301f-1) is arranged above the chip cover plate (301b), the upper end of the second knob (301e-2) is arranged above the chip cover plate (301b), a containing space for conveniently rotating the first knob (301f-1) and the second knob (301e-2) is formed between the downward end of the detection shell (301a) and the upper side of the chip cover plate (301b), and the detection shell (301a) is provided with a third mounting hole which has the same axle center as the first mounting hole (301b-3) (301a-5) and a fourth mounting hole (301a-4) coaxial with the second mounting hole (301b-2), wherein a connecting bracket (311) capable of moving relative to one end of the accommodating box (302) is connected to the motor bracket (315), a first transmission motor (304) and a second transmission motor (317) which are arranged at intervals in the height direction are connected to the connecting bracket (311), a first transmission shaft (319) is connected to the first transmission motor (304), a second transmission shaft (318) is connected to the second transmission motor (317), a first transmission groove (319a) capable of penetrating through the third mounting hole (301a-5) and then being inserted into the first knob (301f-1) is formed in one end of the first transmission shaft (319) arranged relative to the accommodating box (302), a second knob (301e) capable of penetrating through the fourth mounting hole (301a-4) and then being inserted into the second knob (301 e-4) is formed in one end of the second transmission shaft (318) arranged relative to the accommodating box (302) 2) And a second drive groove (318 a).

8. The portable fully automatic microfluidic device according to any one of claims 1 to 3 wherein: a plurality of freeze-dried powder storage chambers (301c-13) which are in one-to-one correspondence with the storage tanks (301c-9) are distributed on the detection chip (301c), the freeze-dried powder storage chambers (301c-13) are communicated with the corresponding storage tanks (301c-9), a fifth liquid outlet channel is arranged on the detection chip (301c) at one end of the freeze-dried powder storage chambers (301c-13) far away from the storage tanks (301c-9), a toxicity detection pool (301c-11) is arranged on the detection chip (301c) at one end of the fifth liquid outlet channel far away from the freeze-dried storage chambers, a water sample inlet channel to be detected is arranged on the detection chip (301c) connected with the outer edge of the toxicity detection pool (301c-11), a first water sample inlet through hole (301c-12) to be detected is arranged on the detection chip (301c) at one end of the water sample inlet channel to be detected far away from the toxicity detection pool (301c-11), a plurality of second water sample liquid inlet through holes (301b-4) to be detected which correspond to the first water sample liquid inlet through holes (301c-12) to be detected one by one are distributed on the chip cover plate (301b), and the second water sample liquid inlet through holes (301b-4) to be detected are coaxial with the corresponding first water sample liquid inlet through holes (301c-12) to be detected.

9. The method for detecting the heavy metals in the fresh water by using the portable full-automatic microfluidic device as claimed in any one of claims 1 to 7, is characterized in that: comprises the following steps of (a) carrying out,

injecting a first detection solution, a second detection solution, a third detection solution, a fourth detection solution and a fifth detection solution into each storage pool (301c-9) in sequence through a liquid inlet hole (301b-1) by using an injector;

injecting a water sample to be detected into the reaction tank (301c-6) through the second reaction liquid inlet through hole (301a-2) and the first reaction liquid inlet through hole (301c-8) in sequence by using a syringe, and inserting the detection card (301) into the accommodating box (302);

the first linear driver (306) acts to push the injection part (309a) into the second reaction liquid inlet through hole (301a-2), the connecting bracket (311) is pushed, so that the first transmission shaft (319) is inserted into the first knob (301f-1) through the first transmission groove (319a), and the second transmission shaft (318) is inserted into the second knob (301e-2) through the second transmission groove (318 a);

controlling a first transmission motor (304) to act to drive a first micro-fluidic valve (301f) to rotate, so that a first storage pool (301c-9) is communicated with a reaction pool (301c-6), stopping the first transmission motor (304), operating an air pump (314), pushing a reaction reagent in the first storage pool (301c-9) into the reaction pool (301c-6), and stopping the air pump (314);

controlling the first transmission motor (304) to continue to act to drive the first micro-fluidic valve (301f) to continue to rotate, so that the second storage pool (301c-9) is communicated with the reaction pool (301c-6), stopping the first transmission motor (304), operating the air pump (314), pushing the reaction reagent in the second storage pool (301c-9) into the reaction pool (301c-6), and stopping the air pump (314);

repeating the liquid feeding step to sequentially push the reaction reagents in the storage pools (301c-9) into the reaction pools (301 c-6);

after the reaction in the reaction tank (301c-6) reaches a set first time threshold, the second transmission motor (317) acts to enable the third liquid outlet channel (301c-1) to be communicated with the fourth liquid outlet channel (301c-5) through the second liquid outlet groove (301e-1), the second transmission motor (317) stops acting, the air pump (314) acts, the mixed solution after the reaction is pushed into the heavy metal detection tank (301c-3), and the redundant mixed solution enters the waste liquid tank (301 c-4);

the light-emitting support (203) slides outwards to enable the light-emitting port (213a) to be opposite to the heavy metal detection pool (301c-3), the light-emitting lamp tube (214) is electrified, the sleeve (209) rotates to enable the light-shielding sheet (211) capable of emitting corresponding wavelengths to cover the position of the light-emitting port (213a), light irradiates the detector (201) through the heavy metal detection pool (301c-3), the detector (201) detects absorbance, the content relation between the absorbance and the heavy metal is obtained, and the detection is finished.

10. The method for detecting the quality of fresh water by using the portable full-automatic microfluidic device as claimed in any one of claims 1 to 3 or 8, is characterized in that: comprises the following steps of (a) carrying out,

before the chip cover plate (301b) is connected to the upper side of the detection chip (301c), the vibrio qinghaiensis freeze-dried powder is stored in the freeze-dried powder storage chamber (301c-13), the chip cover plate (301b) is connected to the detection chip (301c), and the detection card (301) is assembled;

injecting vibrio qinghaiensis resuscitation liquid into each storage pool (301c-9) through a liquid inlet hole (301b-1) by using an injector, and placing the detection card (301) in a refrigeration environment for storage and standby;

during detection, the detection card (301) is taken out, a water sample to be detected is injected into the water sample to be detected through the water sample inlet through hole by using an injector, the water sample to be detected enters the toxicity detection pool (301c-11), and the detection card (301) is inserted into the containing box (302) after sample introduction is finished;

the injection part (309a) is pushed into the second reaction liquid inlet through hole (301a-2), the air pump (314) acts to push the vibrio qinghaiensis resuscitation liquid into the freeze-dried powder storage chamber (301c-13) from the storage tank (301c-9), the air pump (314) stops acting, after the reaction time reaches a set second time threshold, recovering the vibrio qinghaiensis to form vibrio qinghaiensis liquid, actuating the air pump (314) again, pushing the vibrio qinghaiensis liquid into the toxicity detection pool (301c-11) from the freeze-dried powder storage room (301c-13), waiting for reaching a set third time threshold, the camera bracket (204) slides outwards, so that the light-induced lens is opposite to the toxicity detection pool (301c-11), and the light-induced lens detects the fluorescence luminous intensity of vibrio qinghai in the toxicity detection pool (301c-11), thereby completing the detection of the quality of the fresh water.

Technical Field

The invention relates to the technical field of fresh water quality detection, in particular to a device for automatically detecting the quality of fresh water and a method for detecting the quality of fresh water.

Background

With the development of industrialization, the water source area and the safety situation of drinking water are more and more severe, and the health of residents is seriously threatened. Although the traditional water quality safety detection method has higher precision and sensitivity, the detection steps are complex, and time and labor are wasted. And the toxin components in the water body are various and complex, so that the simultaneous detection of various toxins is difficult to realize.

The method for inhibiting the luminescent bacteria is to determine the influence of the poison on the luminous intensity of the luminescent bacteria by using a sensitive photoelectric measurement system so as to evaluate the existence and the toxic intensity of the poison in the water body. In addition, because the reaction of the bacteria and human body cells to the poison has similarity in certain degree, the indicator used for detecting the water quality toxicity has higher accuracy, and the reaction time is shorter and more sensitive due to the characteristic of single cells. The vibrio qinghai is a strain extracted from the body of fish in Qinghai lake, belongs to freshwater bacteria, can be detected without adding NaCl to 3 percent of concentration to a freshwater sample, has no defect that marine luminescent bacteria can not directly detect freshwater, has sensitive reaction to external toxic substances, and has wider application prospect for detecting comprehensive toxicity of freshwater. However, in the prior art, the test solution is configured to the optimal state to be tested by manually sampling, and then the water sample to be tested is injected into the test solution for reaction, but the method has low efficiency and low integration level, and is easily interfered by unknown factors to reduce the accuracy.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made in view of the above and/or other problems occurring in the conventional fresh water quality inspection.

Therefore, the invention aims to provide a portable full-automatic microfluidic device and a detection method thereof, which are convenient to detect, can realize the detection of the quality of fresh water and heavy metals in the fresh water, and are not easy to interfere in the detection.

In order to solve the technical problems, the invention provides the following technical scheme: a portable full-automatic micro-fluidic device and a detection method thereof, which comprises the following steps,

the integrated assembly comprises an integrated box with an upward opening, and the upper side of the integrated box is connected with a box cover capable of being opened and closed;

the reaction assembly is arranged in the integrated box and comprises a motor support and an accommodating box, a detection card is detachably connected in the accommodating box and comprises a detection cover plate, a mounting groove is formed in the detection cover plate, a detection chip is connected in the mounting groove of the detection cover plate, a chip cover plate is connected on the detection chip, a detection shell is connected on the detection cover plate, a plurality of storage pools are distributed on the upper portion of the detection chip, a plurality of liquid inlet holes which are in one-to-one correspondence with the storage pools are distributed on the chip cover plate, a plurality of connecting holes which are in one-to-one correspondence with the liquid inlet holes are distributed on the detection shell, a first air pipe plate which is adjustable in position and is arranged at one end of the accommodating box relatively is connected on the motor support, and a plurality of gas injection valves which are in one-to-one correspondence with the connecting holes are distributed on the first air pipe plate, when the gas injection valve is inserted into the corresponding connecting hole, the inner cavity of the gas injection valve is communicated with the corresponding liquid inlet hole;

the detection assembly is arranged in the integrated box and comprises a detector, a position-adjustable light-emitting support and a camera support, wherein the detector is connected to the motor support, the other end of the box is arranged on the detector, a light-emitting lamp tube is connected to the light-emitting support, the light-emitting lamp tube can be arranged right opposite to the detector, and a light-sensing lens is connected to the camera support.

As a preferable aspect of the portable full-automatic microfluidic device of the present invention, wherein: the motor support with the two ends facing outwards is fixedly connected with at least one fixing column and a first linear driver, the fixing column penetrates through a first air tube plate and then is fixedly connected with a second air tube plate, the first air tube plate is arranged between the second air tube plate and the accommodating box, the first linear driver is connected with a push-pull rod capable of performing reciprocating horizontal linear movement, and the end, extending outwards, of the push-pull rod is connected with the first air tube plate.

As a preferable aspect of the portable full-automatic microfluidic device of the present invention, wherein: the reaction assembly is characterized by further comprising an air pump fixed in the integrated box, wherein an air output base is connected to the air pump, air outlet holes the same as the liquid inlet holes in quantity are distributed in the air output base, a pipeline is connected to the air outlet holes of the air output base, the end of the air injection valve is connected with an injection part, and one end, far away from the air output base, of the pipeline is connected with a corresponding air injection valve.

As a preferable aspect of the portable full-automatic microfluidic device of the present invention, wherein: the LED lamp comprises a light-emitting support and is characterized in that a first mounting cavity is formed in the light-emitting support, a lamp tube support is fixedly connected in the first mounting cavity of the light-emitting support, a light-emitting lamp tube is connected in the lamp tube support, a light-emitting opening is formed in the lamp tube support, a sleeve is rotatably connected on the light-emitting support, the lamp tube support is arranged in the sleeve, and a plurality of light-shading sheets are arranged on the outer side of the sleeve.

As a preferable aspect of the portable full-automatic microfluidic device of the present invention, wherein: a plurality of first liquid outlet channels which are in one-to-one correspondence with the storage pool are distributed on the detection chip, a shunt valve slot is arranged on the detection chip at one end of the first liquid outlet channel far away from the storage pool, a second liquid outlet channel is arranged on the detection chip at one end of the shunt valve groove far away from the first liquid outlet channel, a reaction tank is arranged on the detection chip at one end of the second liquid outlet channel far away from the storage tank, a third liquid outlet channel is arranged on the detection chip at one end of the reaction tank far away from the second liquid outlet channel, a reaction valve groove is formed in the detection chip at one end, away from the reaction tank, of the third liquid outlet channel, a fourth liquid outlet channel is formed in the detection chip at one end, away from the third liquid outlet channel, of the reaction valve groove, a heavy metal detection tank and a waste liquid tank are arranged on the detection chip at one end, away from the reaction valve groove, of the fourth liquid outlet channel, and the heavy metal detection tank and the waste liquid tank can be respectively communicated with the reaction tank; it has the first reaction feed liquor through-hole with the reaction tank intercommunication to open on the detection chip, it has the second reaction feed liquor through-hole with the axle center with first reaction feed liquor through-hole to open on the chip apron.

As a preferable aspect of the portable full-automatic microfluidic device of the present invention, wherein: the detection chip is rotatably connected with a first micro-fluidic valve through a flow dividing valve groove, the first micro-fluidic valve is provided with a first liquid outlet groove, when the first liquid outlet groove is communicated with any liquid outlet channel, a second liquid outlet channel is communicated with the corresponding first liquid outlet groove, and when the first liquid outlet groove is staggered with any first liquid outlet channel, the first liquid outlet channel is closed; the detection chip is rotatably connected with a second micro-fluidic valve through a reaction valve slot, a second liquid outlet slot is formed in the second micro-fluidic valve, a third liquid outlet channel is communicated with a fourth liquid outlet channel through the second liquid outlet slot, when the second liquid outlet slot is staggered with the third or fourth liquid outlet channel, one end, far away from the reaction tank, of the third liquid outlet channel is closed, and one end, opposite to the reaction valve slot, of the fourth liquid outlet channel is also closed.

As a preferable aspect of the portable full-automatic microfluidic device of the present invention, wherein: the upper end of the first micro-fluidic valve is provided with a first knob, the upper end of the second micro-fluidic valve is provided with a second knob, the chip cover plate is provided with a first mounting hole which can just allow the first micro-fluidic valve to pass and a second mounting hole which allows the second micro-fluidic valve to pass, the upper end of the first knob is arranged above the chip cover plate, the upper end of the second knob is arranged above the chip cover plate, a containing space which is used for conveniently rotating the first knob and the second knob is arranged between the downward end of the detection shell and the upper side of the chip cover plate, the detection shell is provided with a third mounting hole which is coaxial with the first mounting hole and a fourth mounting hole which is coaxial with the second mounting hole, the motor bracket is connected with a connecting bracket which can move relative to one end of the containing box, and the connecting bracket is connected with a first transmission motor and a second transmission motor which are arranged at intervals in the height direction, the first transmission motor is connected with a first transmission shaft, the second transmission motor is connected with a second transmission shaft, one end of the first transmission shaft, which is opposite to the accommodating box, is provided with a first transmission groove which can penetrate through the third mounting hole and then is inserted into the first knob, and one end of the second transmission shaft, which is opposite to the accommodating box, is provided with a second transmission groove which can penetrate through the fourth mounting hole and then is inserted into the second knob.

As a preferable aspect of the portable full-automatic microfluidic device of the present invention, wherein: the detection chip is provided with a plurality of freeze-dried powder storage chambers in one-to-one correspondence with the storage tanks, the freeze-dried powder storage chambers are communicated with the corresponding storage tanks, a fifth liquid outlet channel is arranged on the detection chip at one end, away from the storage tanks, of the freeze-dried powder storage chambers, a toxicity detection tank is arranged on the detection chip at one end, away from the freeze-dried storage chambers, of the fifth liquid outlet channel, a water sample inlet channel to be detected is arranged on the detection chip connected with the outer edge of the toxicity detection tank, a first water sample inlet through hole to be detected is formed in the detection chip at one end, away from the toxicity detection tank, of the water sample inlet channel to be detected, a plurality of second water sample inlet through holes to be detected in one-to-one correspondence with the first water sample inlet through holes to be detected are arranged on the chip cover plate, and the second water sample inlet through holes to be detected are coaxial with the corresponding first water sample inlet through holes to be detected.

A method for detecting the quality of fresh water by using a portable full-automatic microfluidic device is disclosed, wherein the method comprises the following steps: comprises the following steps of (a) carrying out,

sequentially injecting a first detection solution, a second detection solution, a third detection solution, a fourth detection solution and a fifth detection solution into each storage pool through a liquid inlet and gas inlet hole by using an injector;

injecting a water sample to be detected into the reaction tank through the second reaction liquid inlet through hole and the first reaction liquid inlet through hole in sequence by using an injector, and inserting the detection card into the containing box;

the first linear driver acts to push the injection part into the second reaction liquid inlet through hole, the connecting bracket is pushed, the first transmission shaft is inserted into the first knob through the first transmission groove, and the second transmission shaft is inserted into the second knob through the second transmission groove;

controlling the action of a first transmission motor to drive a first microfluidic valve to rotate so as to enable a first storage pool to be communicated with a reaction pool, stopping the action of the first transmission motor, actuating an air pump to push a reaction reagent in the first storage pool into the reaction pool, and stopping the action of the air pump;

controlling the first driving motor to continue to act to drive the first microfluidic valve to continue to rotate so as to enable the second storage pool to be communicated with the reaction pool, stopping the first driving motor to act, pushing the reaction reagent in the second storage pool into the reaction pool by the action of the air pump, and stopping the action of the air pump;

repeating the liquid inlet step to sequentially push the reaction reagents in the storage tanks into the reaction tanks;

after the reaction in the reaction tank reaches a set first time threshold, the second transmission motor acts to enable the third liquid outlet channel to be communicated with the fourth liquid outlet channel through the second liquid outlet groove, the second transmission motor stops acting, the air pump acts, the reacted mixed solution is pushed into the heavy metal detection tank, and the redundant mixed solution enters the waste liquid tank;

the light-emitting support outwards slides out, so that the light-emitting opening is just opposite to the heavy metal detection pool, the light-emitting lamp tube is electrified, the sleeve rotates, the light-shading sheet capable of emitting corresponding wavelengths covers the position of the light-emitting opening, light irradiates the detector through the heavy metal detection pool, the detector detects absorbance, the content relation between the absorbance and the heavy metal is obtained, and the detection is finished.

A method for detecting the quality of fresh water by using a portable full-automatic microfluidic device is disclosed, wherein the method comprises the following steps: comprises the following steps of (a) carrying out,

before the chip cover plate is connected to the upper side of the detection chip, the vibrio qinghai freeze-dried powder is stored in the freeze-dried powder storage chamber, the chip cover plate is connected to the detection chip, and the detection card is assembled;

injecting vibrio qinghaiensis recovery liquid into each storage tank through a liquid inlet and gas inlet hole by using an injector, and storing the detection card in a refrigeration environment for later use;

during detection, the detection card is taken out, a water sample to be detected is injected into the detection card through the water sample inlet through hole by using an injector, the water sample to be detected enters the toxicity detection pool, and the detection card is inserted into the accommodating box after sample introduction is finished;

the injection part is pushed into the second reaction liquid inlet through hole, the air pump acts, the vibrio qinghaiensis recovery liquid is pushed into the freeze-dried powder storage chamber from the storage tank, the air pump stops acting, after the reaction time reaches the set second time threshold, the vibrio qinghaiensis is recovered to form vibrio qinghaiensis liquid, the air pump acts again, the vibrio qinghaiensis liquid is pushed into the toxicity detection tank from the freeze-dried powder storage chamber, after the set third time threshold is reached, the camera support slides outwards, the optical sensing lens is enabled to slide out of the toxicity detection tank, the fluorescence luminous intensity of the vibrio qinghaiensis in the toxicity detection tank is detected by the optical sensing lens, and the detection of the quality of the fresh water is completed.

The invention has the beneficial effects that: the invention has high integration level, can realize the detection of the quality of fresh water and the heavy metals in the fresh water, the detection solution is stored in the storage tank on the detection chip, and the detection solution is automatically pushed backwards in sequence according to the liquid inlet sequence during the detection, thereby being not easily influenced by the external environment and improving the detection precision.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive labor. Wherein:

fig. 1 is a perspective view of the first embodiment.

Fig. 2 is a first perspective view of the first embodiment with the cover hidden.

Fig. 3 is a second perspective view of the first embodiment with the cover hidden.

Fig. 4 is a three-dimensional structure view of the first embodiment with the cover hidden.

Fig. 5 is a three-dimensional structure view of the moving rod and the motor bracket fixed together in the invention.

Fig. 6 is a perspective view of the detection card in the first embodiment.

Fig. 7 is an exploded view of the first embodiment of the detection card.

Fig. 8 is an exploded view of the detection card in the first embodiment.

Fig. 9 is a structural view showing a pneumatic pump and a gas output base which are connected together in the present invention.

Fig. 10 is a perspective view showing the light emitting holder and the camera holder being pushed outward according to the present invention.

Fig. 11 is a perspective view of a structure for realizing a light emitting function in the present invention.

Fig. 12 is an exploded view of a structure for realizing a light emitting function in the present invention.

Fig. 13 is a perspective view of a detection card in the second embodiment.

Fig. 14 is an exploded view of the first embodiment of the detection card.

Fig. 15 is an exploded view of the second embodiment of the test card.

FIG. 16 is a graph showing the relationship between absorbance and arsenic content in a test water sample.

FIG. 17 is a graph showing the relationship between absorbance and cadmium content in a test water sample.

FIG. 18 is a graph of absorbance versus the amount of mercury in the test water sample.

FIG. 19 is a graph showing the relationship between absorbance and the amount of zinc in a sample.

FIG. 20 is a graph showing the relationship between absorbance and copper content in a sample.

In the figure, 100 is an integrated assembly, 101 is a handle, 102 is a box cover, 102 is a connecting port, 103 is an integrated box, 200 is a detection assembly, 201 is a detector, 202 is a detection seat, 203 is a light-emitting bracket, 204 is a camera bracket, 205 is a first telescopic rod, 206 is a first position adjusting driver, 207 is a second position adjusting driver, 208 is a second telescopic rod, 209 is a sleeve pipe, 210 is a light-emitting adjusting motor, 211 is a shading sheet, 212 is a transmission belt, 213 is a lamp tube bracket, 213a light-emitting port, 214 is a light-emitting lamp tube, 215 is a driven wheel, 216 is an output shaft, 217 is a driving wheel, 300 is a reaction assembly, 301 is a detection card, 301a is a detection shell, 301a-1 is a connecting hole, 301a-2 is a second reaction liquid inlet through hole, 301a-3 is a second detection port, 301a-4 is a fourth mounting hole, 301a-5 is a third mounting hole, 301a-6 is a liquid sample inlet through hole to be detected, 301a-7 detection hole, 301b chip cover plate, 301b-1 liquid inlet hole, 301b-2 second mounting hole, 301b-3 first mounting hole, 301b-4 second to-be-detected water sample liquid inlet through hole, 301c detection chip, 301c-1 third liquid outlet channel, 301c-2 reaction valve groove, 301c-3 heavy metal detection pool, 301c-4 waste liquid pool, 301c-5 fourth liquid outlet channel, 301c-6 reaction pool, 301c-7 second liquid outlet channel, 301c-8 first reaction liquid inlet through hole, 301c-9 storage pool, 301c-10 first liquid outlet channel, 301c-11 toxicity detection pool, 301c-12 first to-be-detected water sample liquid inlet through hole, 301c-13 freeze-dried powder, 301d detection cover plate, 301d-1 mounting groove, 301d-2 third detection port, 301e second microfluidic valve, 301e-1 second effluent tank, 301e-2 second knob, 301f first microfluidic valve, 301f-1 first knob, 310f-2 first effluent tank, 302 containing box, 302a first detection port, 303 second linear actuator, 304 first driving motor, 305 push-pull rod, 306 first linear actuator, 307 second gas tube plate, 308 first gas tube plate, 309 gas injection valve, 309a injection part, 310 pipe, 311 connecting bracket, 312 moving rod, 313 third linear actuator, 314 gas pump, 315 motor bracket, 316 fixed column, 317 second driving motor, 318 second driving shaft, 318a second driving groove, 319 first driving shaft, 319a first driving groove, 320 gas output base, 320a gas outlet hole.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

Example 1

Referring to fig. 1 to 12, a first embodiment of the present invention provides a portable fully automatic microfluidic device, which is convenient for detection and can detect heavy metals in fresh water.

A portable full-automatic microfluidic device comprises an integrated assembly 100, wherein the integrated assembly 100 comprises an integrated box 103 with an upward opening, a box cover 102 capable of being opened and closed is connected to the upper side of the integrated box 103, a handle 101 capable of conveniently opening and closing the box cover 102 is connected to one upward end of the box cover 102, and a reaction assembly 300 and a detection assembly 200 are installed in the integrated box 103.

Further, the reaction assembly 300 comprises a motor support 315, a containing box 302 and an air pump 314 fixed in the integration box 103, two second linear drivers 303 are fixedly connected in the integration box 103, a lifting rod capable of reciprocating and linear movement in the height direction is connected on the second linear drivers 303, one end of the lifting rod extending upwards is fixedly connected with the containing box 302, a connecting port 102a capable of allowing the containing box 302 to penetrate upwards is formed on the box cover 102, the detection card 301 is detachably connected in the containing box 302, the detection card 301 comprises a detection cover plate 301d, a mounting groove 301d-1 is formed on the detection cover plate 301d, a detection chip 301c is connected in the mounting groove 301d-1 of the detection cover plate 301d, a chip cover plate 301b is connected on the upper side of the detection chip 301c, a detection shell 301a is connected on the upper side of the detection cover plate 301d, and a plurality of storage pools 301c-9 are distributed on the upper portion of the detection chip 301c, a plurality of liquid inlet holes 301b-1 which are in one-to-one correspondence with the storage pool 301c-9 are distributed on the chip cover plate 301b, a plurality of connecting holes 301a-1 which are in one-to-one correspondence with the liquid inlet holes 301b-1 are distributed on the detection shell 301a, a first air tube plate 308 which is adjustable in position and is arranged opposite to one end of the containing box 302 is connected on the motor support 315, a plurality of gas injection valves 309 which are in one-to-one correspondence with the connecting holes 301a-1 are distributed on the first air tube plate 308, an injection part 309a is arranged at the end part of each gas injection valve 309 and can be inserted into the liquid inlet hole 301b-1, a gas output base 320 is connected on the air pump 314, gas outlet holes 320a which are the same in number as the liquid inlet holes 301b-1 are distributed on the gas output base 320, and a pipeline 310 is connected at the gas outlet hole 320a of the gas output base 320, the end of the conduit 310 remote from the gas output base 320 is connected to a corresponding gas injection valve 309.

Further, the detecting assembly 200 is disposed in the integrated box 103, the detecting assembly 200 includes a detector 201 connected to the motor bracket 315, a position-adjustable light-emitting bracket 203 and a camera bracket 204, the motor bracket 315 is fixedly connected to the detecting base 202, the detecting base 202 has two independent first and second mounting cavities, the first and second mounting cavities are respectively provided with a first position adjusting driver 206 and a second position adjusting driver 208207, the first position adjusting driver 206 is connected to a first telescopic rod 205 capable of performing reciprocating linear movement in the front-rear direction, an end of the first telescopic rod 205 is connected to the light-emitting bracket 203, the second position adjusting driver 208207 is connected to a second telescopic rod capable of performing reciprocating linear movement in the front-rear direction, an end of the second telescopic rod is connected to the camera bracket 204, the detector 201 is disposed opposite to the other end of the accommodating box 302, the light-emitting bracket 203 is connected with a light-emitting lamp tube 214, the light-emitting lamp tube 214 can be arranged opposite to the detector 201, the light-emitting bracket 203 is provided with a first mounting cavity and a second mounting cavity, the first mounting cavity of the light-emitting bracket 203 is fixedly connected with a lamp tube bracket 213, the lamp tube bracket 213 is connected with the light-emitting lamp tube 214, the lamp tube bracket 213 is provided with a light-emitting port 213a, the light-emitting bracket 203 is rotatably connected with a sleeve 209, the lamp tube bracket 213 is arranged in the sleeve 209, a plurality of light-shading sheets 211 capable of shading the light-emitting port 213a are arranged outside the sleeve 209, the conversion of light sources with wavelengths of 555nm, 558nm, 532nm, 620nm and 457nm is realized through the arrangement of different light-shading sheets 211, the second mounting cavity of the light-emitting bracket 203 is fixedly connected with a light-emitting adjusting motor 210, the light-emitting adjusting motor 210 is connected with an output shaft 216, the output shaft 216 is connected with a driving wheel 217, the end part of the sleeve 209 is connected with a driven wheel 215, the driving wheel 217 is in transmission connection with the driven wheel 215, in this embodiment, the driving wheel 217 is connected with the driven wheel 215 through the transmission belt 212, and the camera holder 204 is connected with a light-sensing lens.

Furthermore, the motor brackets 315 at two ends of the accommodating box 302 facing outward are fixedly connected with at least one fixed column 316 and a first linear actuator 306, the fixed column 316 penetrates through the first air tube plate 308 and then is fixedly connected to the second air tube plate 307, the first air tube plate 308 is arranged between the second air tube plate 307 and the accommodating box 302, the first linear actuator 306 is connected with a push-pull rod 305 capable of moving back and forth in a horizontal linear direction, and one end of the push-pull rod 305 extending outward is connected with the first air tube plate 308.

Furthermore, a plurality of first liquid outlet channels 301c-10 which are in one-to-one correspondence with the storage pool 301c-9 are distributed on the detection chip 301c, a shunt valve groove is arranged on the detection chip 301c at the end of the first liquid outlet channel 301c-10 far away from the storage pool 301c-9, a second liquid outlet channel 301c-7 is arranged on the detection chip 301c at the end of the shunt valve groove far away from the first liquid outlet channel 301c-10, a reaction pool 301c-6 is arranged on the detection chip 301c at the end of the second liquid outlet channel 301c-7 far away from the storage pool 301c-9, a third liquid outlet channel 301c-1 is arranged on the detection chip 301c at the end of the reaction pool 301c-6 far away from the second liquid outlet channel 301c-7, a reaction valve groove 301c-2 is arranged on the detection chip 301c at the end of the third liquid outlet channel 301c-1 far away from the reaction pool 301c-6, a fourth liquid outlet channel 301c-5 is arranged on the detection chip 301c at the end of the reaction valve groove 301c-2 far away from the third liquid outlet channel 301c-1, a heavy metal detection pool 301c-3 and a waste liquid pool 301c-4 are arranged on the detection chip 301c at the end of the fourth liquid outlet channel 301c-5 far away from the reaction valve groove 301c-2, a first detection port 302a corresponding to the heavy metal detection pool 301c-3 is arranged at one end of the containing box 302 opposite to the detector 201, the first detection port 302a covers the area where the heavy metal detection pool 301c-3 is located, the detector 201 faces the first detection port 302a, and the heavy metal detection pool 301c-3 and the waste liquid pool 301c-4 can be respectively communicated with the reaction pool 301 c-6; a first reaction liquid inlet through hole 301c-8 communicated with the reaction tank 301c-6 is formed in the detection chip 301c, and a second reaction liquid inlet through hole 301a-2 coaxial with the first reaction liquid inlet through hole 301c-8 is formed in the chip cover plate 301 b; the detection shell 301a and the detection cover plate 301d are respectively provided with a second detection port 301a-3 and a third detection port 301d-2 which are opposite to the heavy metal detection pool 301 c-3.

Further, the detection chip 301c is rotatably connected with a first micro-fluidic valve 301f through a shunt valve slot, the first micro-fluidic valve 301f is provided with a first liquid outlet slot 301f-2, when the first liquid outlet slot 301f-2 is communicated with any liquid outlet channel, the second liquid outlet channel 301c-7 is communicated with the corresponding first liquid outlet slot 301f-2, and when the first liquid outlet slot 301f-2 is staggered with any first liquid outlet channel 301c-10, the first liquid outlet channel 301c-10 is closed; the detection chip 301c is rotatably connected with a second micro-fluidic valve 301e through a reaction valve slot 301c-2, the second micro-fluidic valve 301e is provided with a second liquid outlet slot 301e-1, a third liquid outlet channel 301c-1 is communicated with a fourth liquid outlet channel 301c-5 through the second liquid outlet slot 301e-1, when the second liquid outlet slot 301e-1 is staggered with the third or fourth liquid outlet channel 301c-5, one end of the third liquid outlet channel 301c-1, which is far away from the reaction pool 301c-6, is closed, and one end of the fourth liquid outlet channel 301c-5, which is opposite to the reaction valve slot 301c-2, is also closed.

Further, a first knob 301f-1 is arranged at the upper end of the first micro-fluidic valve 301f, a second knob 301e-2 is arranged at the upper end of the second micro-fluidic valve 301e, a first mounting hole 301b-3 through which the first micro-fluidic valve 301f can just pass and a second mounting hole 301b-2 through which the second micro-fluidic valve 301e can pass are arranged on the chip cover plate 301b, the upper end of the first knob 301f-1 is arranged above the chip cover plate 301b, the upper end of the second knob 301e-2 is arranged above the chip cover plate 301b, a containing space for conveniently rotating the first knob 301f-1 and the second knob 301e-2 is arranged between the downward end of the detection shell 301a and the upper side of the chip cover plate 301b, a third mounting hole 301a-5 coaxial with the first mounting hole 301b-3 and a fourth mounting hole 301a-4 coaxial with the second mounting hole 301b-2 are arranged on the detection shell 301a, the motor bracket 315 is connected with a connecting bracket 311 which can move relative to one end of the accommodating box 302, the connecting bracket 311 is connected with a first transmission motor 304 and a second transmission motor 317 which are arranged at intervals in the height direction, the first transmission motor 304 is connected with a first transmission shaft 319, the second transmission motor 317 is connected with a second transmission shaft 318, one end of the first transmission shaft 319, which is arranged relative to the accommodating box 302, is provided with a first transmission groove 319a which can penetrate through a third mounting hole 301a-5 and then be inserted on a first knob 301f-1, one end of the second transmission shaft 318, which is arranged relative to the accommodating box 302, is provided with a second transmission groove 318a which can penetrate through a fourth mounting hole 301a-4 and then be inserted on a second knob 301e-2, the connecting bracket 311 between the first transmission motor 304 and the second transmission motor 317 is fixedly connected with a third linear driver 313, the third linear driver 313 is connected with a moving rod 312 which extends outwards and can do reciprocating linear movement, the end of the travel bar 312 is attached to a motor bracket 315.

The first linear driver 306, the second linear driver 303 and the third linear driver 313 are all the prior art; when heavy metals in the fresh water quality are detected, the third linear driver 313 acts to enable the moving rod 312 to extend outwards, the connecting support 311 is pushed to move towards the accommodating box 302, the first transmission shaft 319 is inserted into the first knob 301f-1 through the first transmission groove 319a, the first transmission motor 304 is controlled to act, the first transmission shaft 319 rotates, the first transmission shaft 319 drives the first knob 301f-1 to rotate, the first knob 301f-1 drives the first micro-fluidic valve 301f to rotate, the first liquid outlet groove 301f-2 is communicated with the first liquid outlet channel 301c-10 and the second liquid outlet channel 301c-7, the first transmission motor 304 stops acting, the air pump 314 acts, the corresponding injection part 309a is opened, air is injected into the first storage pool 301c-9, the air pump 314 stops acting, and detection solutions in the first storage pool 301c-9 are pushed to sequentially pass through the first liquid outlet channel 301c-10, the second liquid outlet channel 301c-7, The first liquid outlet channel 301f-2 and the second liquid outlet channel 301c-7 enter the reaction tank 301c-6, the first driving motor 304 continues to operate to communicate the first liquid outlet channel 301f-2 with the next liquid outlet channel and the second liquid outlet channel 301c-7, the first driving motor 304 stops operating, the air pump 314 operates, the next pneumatic injection valve is opened, the detection solution in the second storage tank 301c-9 sequentially enters the reaction tank 301c-6 through the first liquid outlet channel 301c-10, the first liquid outlet channel 301f-2 and the second liquid outlet channel 301c-7, the two detection solutions react, the first driving motor 304 continues to operate to rotate the first liquid outlet channel 301f-2 towards the third first liquid outlet channel 301c-10, when the third first liquid outlet channel 301c-10 is communicated with the first liquid outlet channel 301f-2, stopping the action of the first driving motor 304, actuating the air pump 314, opening the corresponding injection part 309a, injecting gas into the third storage pool 301c-9, pushing the detection solution in the third storage pool 301c-9 into the reaction pool 301c-6, repeating the above steps to sequentially push the detection solution in the five storage pools 301c-9 into the reaction pool 301c-6, after the reaction in the reaction pool 301c-6 for a period of time, actuating the second driving motor 317, driving the second micro-fluidic valve 301e to rotate by the second knob 301e-2 to communicate the second liquid outlet tank 301e-1 with the third liquid outlet channel 301c-1 and the fourth liquid outlet channel 301c-5, actuating the air pump 314, opening the last injection part 309a, pushing the reacted solution into the heavy metal detection pool 301c-3, excess solution flows into waste reservoir 301 c-4.

During detection, the first position adjusting driver 206 acts to extend the first telescopic rod 205 outwards, the light emitting bracket 203 is pushed out towards the heavy metal detection pool 301c-3, the light emitting opening 213a is opposite to the heavy metal detection pool 301c-3, the light emitting bracket 203 stops moving, the light emitting adjusting motor 210 acts, the driving gear rotates, the driving gear drives the driven gear to rotate, the driven gear drives the sleeve 209 to rotate, the sleeve 209 drives the light shielding sheet 211 to rotate, when the required light shielding sheet 211 covers the position of the light emitting opening 213a, the light emitting adjusting motor 210 stops acting, the lamp tube is electrified, light emitted by the lamp tube passes through the light emitting opening 213a and the light shielding sheet 211 to irradiate on the heavy metal detection pool 301c-3 area, the light passes through the heavy metal detection pool 301c-3 to irradiate on the detector 201, the detector 201 detects absorbance, and the absorbance detection result is converted into heavy metal content, the detection of the content of heavy metals in fresh water is realized, the detection is convenient, the influence of the external environment is not easy, and the detection precision is improved.

Example 2

Referring to fig. 13 to 15, a second embodiment of the present invention is different from the first embodiment in that the second embodiment provides a portable fully automatic microfluidic device capable of detecting the quality of fresh water.

A portable full-automatic micro-fluidic device comprises a plurality of freeze-dried powder storage chambers 301c-13 which are arranged on a detection chip 301c and correspond to storage pools 301c-9 one by one, the freeze-dried powder storage chambers 301c-13 are communicated with the corresponding storage pools 301c-9, a fifth liquid outlet channel is arranged on the detection chip 301c at one end of the freeze-dried powder storage chambers 301c-13 far away from the storage pools 301c-9, a toxicity detection pool 301c-11 is arranged on the detection chip 301c at one end of the fifth liquid outlet channel far away from the freeze-dried storage chambers, a plurality of detection holes 301a-7 which correspond to the toxicity detection pool 301c-11 one by one are arranged on a detection shell 301a, the detection holes 301a-7 cover the corresponding toxicity detection pool 301c-11, a liquid inlet channel for a water sample to be detected is arranged on the detection chip 301c which is connected with the outer edges of the toxicity detection pool 301c-11, a first water sample inlet through hole 301c-12 is formed in a detection chip 301c at one end, away from the toxicity detection pool 301c-11, of a water sample inlet channel to be detected, a plurality of second water sample inlet through holes 301b-4 which correspond to the first water sample inlet through holes 301c-12 in a one-to-one manner are distributed in a chip cover plate 301b, a plurality of third water sample inlet through holes 301a-6 which correspond to the second water sample inlet through holes 301b-4 in a one-to-one manner are distributed in a detection shell 301a, and the third water sample inlet through holes 301a-6 and the second water sample inlet through holes 301b-4 to be detected are coaxial with the corresponding first water sample inlet through holes 301c-12 to be detected.

Freeze-dried powder is stored in the freeze-dried powder storage chambers 301c-13, a water sample to be detected is injected into the water sample to be detected through the water inlet through hole of the water sample to be detected by using an injector, the water sample to be detected enters the corresponding toxicity detection tanks 301c-11, the detection liquid is injected into the storage tanks 301c-9 through the liquid inlet holes 301b-1 by using the injector, the air pump 314 acts, all injection parts 309a are opened simultaneously, the detection liquid in each storage tank 301c-9 is pushed into the freeze-dried powder storage chambers 301c-13, the air pump 314 stops acting, the air pump 314 acts again after reacting for a period of time, the reacted solution is pushed into the toxicity detection tanks 301c-11, after waiting for a period of time, the second position adjusting driver 208207 acts to enable the second telescopic rods to extend outwards, the camera support 204 moves towards the direction of the toxicity detection tanks 301c-11, when the light sensing lens faces the toxicity detection tanks 301c-11, the camera bracket 204 stops moving, the light sensing lens detects the fluorescence luminous intensity of the detection liquid in the toxicity detection pool 301c-11, and the detection of the quality of the fresh water is realized by reflecting the quality of the fresh water through the fluorescence luminous intensity.

Example 3

The third embodiment of the present invention is different from the first and second embodiments in that it provides a method for fresh water heavy metal detection using a portable fully-automatic microfluidic device.

The method for detecting the heavy metal in the fresh water by using the portable full-automatic microfluidic device comprises the following steps,

injecting a first detection solution, a second detection solution, a third detection solution, a fourth detection solution and a fifth detection solution into each storage pool 301c-9 in sequence through a liquid inlet hole 301b-1 by using an injector;

injecting a water sample to be detected into the reaction tank 301c-6 through the second reaction liquid inlet through hole 301a-2 and the first reaction liquid inlet through hole 301c-8 in sequence by using a syringe, and inserting the detection card 301 into the accommodating box 302;

the first linear driver 306 is operated to push the injection part 309a into the second reaction liquid inlet through hole 301a-2, the connecting bracket 311 is pushed, the first transmission shaft 319 is inserted into the first knob 301f-1 through the first transmission groove 319a, and the second transmission shaft 318 is inserted into the second knob 301e-2 through the second transmission groove 318 a;

controlling the first transmission motor 304 to act to drive the first microfluidic valve 301f to rotate, so that the first storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first transmission motor 304, and driving the air pump 314 to act to push the reaction reagent in the first storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

controlling the first driving motor 304 to continue to act to drive the first microfluidic valve 301f to continue to rotate, so that the second storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first driving motor 304, and driving the air pump 314 to act to push the reaction reagent in the second storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

repeating the liquid feeding step to sequentially push the first detection solution, the second detection solution, the third detection solution, the fourth detection solution and the fifth detection solution into the reaction tank 301 c-6;

after the reaction in the reaction tank 301c-6 reaches a set first time threshold, the second transmission motor 317 operates to enable the third liquid outlet channel 301c-1 to be communicated with the fourth liquid outlet channel 301c-5 through the second liquid outlet groove 301e-1, the second transmission motor 317 stops operating, the air pump 314 operates, the mixed solution after the reaction is pushed into the heavy metal detection tank 301c-3, and the redundant mixed solution enters the waste liquid tank 301 c-4;

the light emitting bracket 203 slides outwards to enable the light emitting port 213a to be opposite to the heavy metal detection pool 301c-3, the light emitting lamp tube 214 is electrified, the sleeve 209 rotates to enable the light shielding sheet 211 capable of emitting light with corresponding wavelength to cover the position of the light emitting port 213a, the light irradiates the detector 201 through the heavy metal detection pool 301c-3, the detector 201 detects absorbance to obtain the relation between the absorbance and the content of the heavy metal, the second transmission motor 317 drives the first micro-fluidic valve 301f and the second micro-fluidic valve 301e to rotate back to the initial angle, the pipeline 310 retracts to the initial position, the third linear driver 313 operates, the movable rod 312 retracts to the initial position, the third linear driver 313 stops operating, the first transmission shaft 319 and the second transmission shaft 318 leave the detection card 301, the first linear driver 306 operates to enable the push-pull rod 305 to extend outwards, and the pipeline 310 leaves the detection card 301, the first linear actuator 306 stops operating, the second linear actuator 303 operates to extend the lift lever upward, the housing box 302 extends upward out of the connection port 102a, the detection card 301 is taken out, and the detection is completed.

Example 4

Referring to fig. 1 to 3, a third embodiment of the present invention is different from any one of embodiments 1 to 3 in that the present embodiment provides a method for performing fresh water quality using a portable fully-automatic microfluidic device, including the steps of:

before the chip cover plate 301b is connected to the upper side of the detection chip 301c, the Arthrobacter qinghaii freeze-dried powder is stored in the freeze-dried powder storage chamber 301c-13, the chip cover plate 301b is connected to the detection chip 301c, and the detection card 301 is assembled;

injecting vibrio qinghaiensis resuscitation liquid into each storage pool 301c-9 through a liquid inlet hole 301b-1 by using an injector, and storing the detection card 301 in a refrigeration environment for later use;

during detection, the detection card 301 is taken out, a water sample to be detected is injected into the water sample to be detected through the water sample inlet through hole by using an injector, the water sample to be detected enters the toxicity detection pool 301c-11, and the detection card 301 is inserted into the accommodating box 302 after sample introduction is finished;

the injection part 309a is pushed into the second reaction liquid inlet through hole 301a-2, the air pump 314 acts to push the vibrio qinghai recovery liquid into the freeze-dried powder storage chamber 301c-13 from the storage pool 301c-9, the air pump 314 stops acting, after the reaction time reaches a set second time threshold, the vibrio qinghai recovers to form vibrio qinghai bacteria liquid, the air pump 314 acts again to push the vibrio qinghai bacteria liquid into the toxicity detection pool 301c-11 from the freeze-dried powder storage chamber 301c-13, after the set third time threshold is reached, the camera support 204 slides outwards, so that the light-sensitive lens is opposite to the fluorescence intensity of the vibrio qinghai in the toxicity detection pool 301c-11, and the detection of the quality of fresh water is completed.

Example 5

The fifth embodiment of the present invention is different from embodiments 1 to 4 in that this embodiment provides a method for detecting heavy metal arsenic in fresh water using a portable fully-automatic microfluidic device, which can detect heavy metal arsenic in fresh water.

The method for detecting the heavy metal arsenic in the fresh water comprises the following steps,

injecting 20 microliter of mixed solution of 0.5 percent ammonium molybdate and 0.1 percent potassium antimonate, 75 microliter of ascorbic acid solution (0.1mol/L), 100 microliter of arabic gum solution (1.0 percent aqueous solution), 30 microliter of Tween 20 solution (1.0 percent aqueous solution by volume) and 20 microliter of methyl violet solution (0.955 mu g/mL) into each storage pool 301c-9 in sequence through a liquid inlet hole 301b-1 by using a syringe;

injecting a water sample to be detected into the reaction tank 301c-6 through the second reaction liquid inlet through hole 301a-2 and the first reaction liquid inlet through hole 301c-8 in sequence by using a syringe, and inserting the detection card 301 into the accommodating box 302;

the first linear driver 306 is operated to push the injection part 309a into the second reaction liquid inlet through hole 301a-2, the connecting bracket 311 is pushed, the first transmission shaft 319 is inserted into the first knob 301f-1 through the first transmission groove 319a, and the second transmission shaft 318 is inserted into the second knob 301e-2 through the second transmission groove 318 a;

controlling the first transmission motor 304 to act to drive the first microfluidic valve 301f to rotate, so that the first storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first transmission motor 304, and driving the air pump 314 to act to push the reaction reagent in the first storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

controlling the first driving motor 304 to continue to act to drive the first microfluidic valve 301f to continue to rotate, so that the second storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first driving motor 304, and driving the air pump 314 to act to push the reaction reagent in the second storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

repeating the above liquid feeding step to sequentially push the mixed solution of 0.5% ammonium molybdate-0.1% antimony potassium tartrate solution, ascorbic acid solution, Arabic gum solution, Tween 20 solution and methyl violet solution into the reaction tank 301 c-6;

after the reaction is carried out in the reaction tank 301c-6 for 10 minutes, the second transmission motor 317 acts to enable the third liquid outlet channel 301c-1 to be communicated with the fourth liquid outlet channel 301c-5 through the second liquid outlet groove 301e-1, the second transmission motor 317 stops acting, the air pump 314 acts, the mixed solution after the reaction is pushed into the heavy metal detection tank 301c-3, and the redundant mixed solution enters the waste liquid tank 301 c-4;

the light emitting bracket 203 slides outwards to enable the light emitting port 213a to be opposite to the heavy metal detection pool 301c-3, the light emitting lamp tube 214 is electrified, the sleeve 209 rotates to enable the light shielding sheet 211 capable of emitting light with the wavelength of 555nm to cover the position of the light emitting port 213a, the light is irradiated on the detector 201 through the heavy metal detection pool 301c-3, the detector 201 detects the absorbance to obtain the relationship between the absorbance and the content of the heavy metal, the second transmission motor 317 drives the first micro-fluidic valve 301f and the second micro-fluidic valve 301e to rotate back to the initial angle, the pipeline 310 is retracted to the initial position, the third linear driver 313 operates, the movable rod 312 is retracted to the initial position, the third linear driver 313 stops operating, the first transmission shaft 319 and the second transmission shaft 318 leave the detection card 301, the first linear driver 306 operates to enable the push-pull rod to extend outwards, and the pipeline 310 leaves the detection card 301, the first linear actuator 306 stops operating, the second linear actuator 303 operates to extend the lift lever upward, the housing box 302 extends upward out of the connection port 102a, the detection card 301 is taken out, and the detection is completed.

EXAMPLE six

The sixth embodiment of the present invention is different from embodiments 1 to 5 in that the present embodiment provides a method for detecting arsenic, which is a heavy metal in fresh water, using a portable full-automatic microfluidic device, which can realize detection of cadmium, which is a heavy metal in fresh water, and can realize detection of cadmium, which is a heavy metal in fresh water.

The method for detecting the heavy metal arsenic in the fresh water by using the portable full-automatic microfluidic device comprises the following steps:

potassium iodide, namely 60 microliters of ascorbic acid solution (1.0mol/L), 60 microliters of sulfuric acid solution (1.0mol/L), 60 microliters of rhodamine B solution (0.5g/L) and 60 microliters of polyvinyl alcohol-1799 solution (10g/L) are sequentially injected into each storage pool 301c-9 through a liquid inlet hole 301B-1 by using an injector;

injecting a water sample to be detected into the reaction tank 301c-6 through the second reaction liquid inlet through hole 301a-2 and the first reaction liquid inlet through hole 301c-8 in sequence by using a syringe, and inserting the detection card 301 into the accommodating box 302;

the first linear driver 306 is operated to push the injection part 309a into the second reaction liquid inlet through hole 301a-2, the connecting bracket 311 is pushed, the first transmission shaft 319 is inserted into the first knob 301f-1 through the first transmission groove 319a, and the second transmission shaft 318 is inserted into the second knob 301e-2 through the second transmission groove 318 a;

controlling the first transmission motor 304 to act to drive the first microfluidic valve 301f to rotate, so that the first storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first transmission motor 304, and driving the air pump 314 to act to push the reaction reagent in the first storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

controlling the first driving motor 304 to continue to act to drive the first microfluidic valve 301f to continue to rotate, so that the second storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first driving motor 304, and driving the air pump 314 to act to push the reaction reagent in the second storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

repeating the above liquid feeding step to sequentially push potassium iodide, ascorbic acid solution, sulfuric acid solution, rhodamine B solution and polyvinyl alcohol-1799 solution into the reaction tank 301 c-6;

after the reaction is carried out in the reaction tank 301c-6 for 10 minutes, the second transmission motor 317 acts to enable the third liquid outlet channel 301c-1 to be communicated with the fourth liquid outlet channel 301c-5 through the second liquid outlet groove 301e-1, the second transmission motor 317 stops acting, the air pump 314 acts, the mixed solution after the reaction is pushed into the heavy metal detection tank 301c-3, and the redundant mixed solution enters the waste liquid tank 301 c-4;

the light emitting bracket 203 slides outwards to enable the light emitting port 213a to be opposite to the heavy metal detection pool 301c-3, the light emitting lamp tube 214 is electrified, the sleeve 209 rotates to enable the light shielding sheet 211 capable of emitting light with a wavelength of 558nm to cover the position of the light emitting port 213a, the light is irradiated on the detector 201 through the heavy metal detection pool 301c-3, the detector 201 detects absorbance to obtain the relation between the absorbance and the content of the heavy metal, the second transmission motor 317 drives the first micro-fluidic valve 301f and the second micro-fluidic valve 301e to rotate back to the initial angle, the pipeline 310 is retracted to the initial position, the third linear driver 313 operates, the movable rod 312 is retracted to the initial position, the third linear driver 313 stops operating, the first transmission shaft 319 and the second transmission shaft 318 leave the detection card 301, the first linear driver 306 operates to enable the push-pull rod to extend outwards, and the pipeline 310 leaves the detection card 301, the first linear actuator 306 stops operating, the second linear actuator 303 operates to extend the lift lever upward, the housing box 302 extends upward out of the connection port 102a, the detection card 301 is taken out, and the detection is completed.

EXAMPLE seven

The seventh embodiment of the present invention is different from embodiments 1 to 6 in that the present embodiment provides a method for detecting heavy metal mercury in fresh water by using a portable full-automatic microfluidic device, which can realize detection of heavy metal mercury in fresh water and detection of heavy metal mercury in fresh water.

The method for detecting the heavy metal mercury in the fresh water by using the portable full-automatic microfluidic device comprises the following steps:

injecting 40 microliters of nitric acid solution (1.0mol/L), 80 microliters of sodium bromide solution (2.0mol/L), 60 microliters of tween 80 solution (10g/L) and 60 microliters of rhodamine B solution (1.0 multiplied by 10mol/L) into the four adjacent storage pools 301c-9 in sequence through the liquid inlet holes 301B-1 by using an injector;

injecting a water sample to be detected into the reaction tank 301c-6 through the second reaction liquid inlet through hole 301a-2 and the first reaction liquid inlet through hole 301c-8 in sequence by using a syringe, and inserting the detection card 301 into the accommodating box 302;

the first linear driver 306 is operated to push the injection part 309a into the second reaction liquid inlet through hole 301a-2, the connecting bracket 311 is pushed, the first transmission shaft 319 is inserted into the first knob 301f-1 through the first transmission groove 319a, and the second transmission shaft 318 is inserted into the second knob 301e-2 through the second transmission groove 318 a;

controlling the first transmission motor 304 to act to drive the first microfluidic valve 301f to rotate, so that the first storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first transmission motor 304, and driving the air pump 314 to act to push the reaction reagent in the first storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

controlling the first driving motor 304 to continue to act to drive the first microfluidic valve 301f to continue to rotate, so that the second storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first driving motor 304, and driving the air pump 314 to act to push the reaction reagent in the second storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

repeating the liquid feeding step to sequentially push the nitric acid solution, the sodium bromide solution, the Tween 80 solution and the rhodamine B solution into the reaction tank 301 c-6;

after the reaction is carried out in the reaction tank 301c-6 for 10 minutes, the second transmission motor 317 acts to enable the third liquid outlet channel 301c-1 to be communicated with the fourth liquid outlet channel 301c-5 through the second liquid outlet groove 301e-1, the second transmission motor 317 stops acting, the air pump 314 acts, the mixed solution after the reaction is pushed into the heavy metal detection tank 301c-3, and the redundant mixed solution enters the waste liquid tank 301 c-4;

the light emitting bracket 203 slides outwards to enable the light emitting port 213a to be opposite to the heavy metal detection pool 301c-3, the light emitting lamp tube 214 is electrified, the sleeve 209 rotates to enable the shading sheet 211 capable of emitting light with the wavelength of 532nm to cover the position of the light emitting port 213a, the light is irradiated on the detector 201 through the heavy metal detection pool 301c-3, the detector 201 detects the absorbance to obtain the relationship between the absorbance and the content of the heavy metal, the second transmission motor 317 drives the first micro-fluidic valve 301f and the second micro-fluidic valve 301e to rotate back to the initial angle, the pipeline 310 is retracted to the initial position, the third linear driver 313 operates, the movable rod 312 is retracted to the initial position, the third linear driver 313 stops operating, the first transmission shaft 319 and the second transmission shaft 318 leave the detection card 301, the first linear driver 306 operates to enable the push-pull rod to extend outwards, and the pipeline 310 leaves the detection card 301, the first linear actuator 306 stops operating, the second linear actuator 303 operates to extend the lift lever upward, the housing box 302 extends upward out of the connection port 102a, the detection card 301 is taken out, the second linear actuator 303 operates in the reverse direction, the travel lever 312 retracts, the housing box 302 is pulled and retracted, the second linear actuator 303 stops operating, and the detection is completed.

Example eight

The eighth embodiment of the present invention is different from embodiments 1 to 7 in that the present embodiment provides a method for detecting heavy metal zinc in fresh water by using a portable full-automatic microfluidic device, which can realize detection of heavy metal zinc in fresh water, and can realize detection of heavy metal zinc in fresh water.

The method for detecting the heavy metal zinc in the fresh water by using the portable full-automatic microfluidic device comprises the following steps:

87.5 microliters of KSCN solution (50g/L), 21 microliters of hydrochloric acid solution (1: 1 volume aqueous solution), 56 microliters of rhodamine B solution (0.05g/L) and 3.5 microliters of ascorbic acid solution (50g/L) are sequentially injected into the four adjacent storage pools 301c-9 through the liquid inlet holes 301B-1 by using a syringe;

injecting a water sample to be detected into the reaction tank 301c-6 through the second reaction liquid inlet through hole 301a-2 and the first reaction liquid inlet through hole 301c-8 in sequence by using a syringe, and inserting the detection card 301 into the accommodating box 302;

the first linear driver 306 is operated to push the injection part 309a into the second reaction liquid inlet through hole 301a-2, the connecting bracket 311 is pushed, the first transmission shaft 319 is inserted into the first knob 301f-1 through the first transmission groove 319a, and the second transmission shaft 318 is inserted into the second knob 301e-2 through the second transmission groove 318 a;

controlling the first transmission motor 304 to act to drive the first microfluidic valve 301f to rotate, so that the first storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first transmission motor 304, and driving the air pump 314 to act to push the reaction reagent in the first storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

controlling the first driving motor 304 to continue to act to drive the first microfluidic valve 301f to continue to rotate, so that the second storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first driving motor 304, and driving the air pump 314 to act to push the reaction reagent in the second storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

repeating the liquid feeding step to sequentially push the KSCN solution, the hydrochloric acid solution, the rhodamine B solution and the ascorbic acid solution into the reaction tank 301 c-6;

after reacting for 15 minutes in the reaction tank 301c-6, the second transmission motor 317 acts to enable the third liquid outlet channel 301c-1 to be communicated with the fourth liquid outlet channel 301c-5 through the second liquid outlet groove 301e-1, the second transmission motor 317 stops acting, the air pump 314 acts, the mixed solution after the reaction is pushed into the heavy metal detection tank 301c-3, and the redundant mixed solution enters the waste liquid tank 301 c-4;

the light emitting bracket 203 slides outwards to enable the light emitting port 213a to be opposite to the heavy metal detection pool 301c-3, the light emitting lamp tube 214 is electrified, the sleeve 209 rotates to enable the light shielding sheet 211 capable of emitting light with the wavelength of 620nm to cover the position of the light emitting port 213a, the light is irradiated on the detector 201 through the heavy metal detection pool 301c-3, the detector 201 detects the absorbance to obtain the relationship between the absorbance and the content of the heavy metal, the second transmission motor 317 drives the first micro-fluidic valve 301f and the second micro-fluidic valve 301e to rotate back to the initial angle, the pipeline 310 is retracted to the initial position, the third linear driver 313 operates, the movable rod 312 is retracted to the initial position, the third linear driver 313 stops operating, the first transmission shaft 319 and the second transmission shaft 318 leave the detection card 301, the first linear driver 306 operates to enable the push-pull rod to extend outwards, and the pipeline 310 leaves the detection card 301, the first linear actuator 306 stops operating, the second linear actuator 303 operates to extend the lift lever upward, the housing box 302 extends upward out of the connection port 102a, the detection card 301 is taken out, the second linear actuator 303 operates in the reverse direction, the travel lever 312 retracts, the housing box 302 is pulled and retracted, the second linear actuator 303 stops operating, and the detection is completed.

Example nine

The ninth embodiment of the present invention is different from embodiments 1 to 8 in that the present embodiment provides a method for detecting heavy metal copper in fresh water by using a portable full-automatic microfluidic device, which can realize detection of heavy metal copper in fresh water and detection of heavy metal copper in fresh water.

The method for detecting the heavy metal copper in the fresh water by using the portable full-automatic microfluidic device comprises the following steps:

15 microliters of hydroxylamine hydrochloride solution (100g/L), 30 microliters of sodium citrate solution (375g/L), 30 microliters of acetic acid-sodium acetate buffer solution (pH is 5.7), and 15 microliters of cuprous chloride solution (2g/L) are sequentially injected into the four adjacent storage pools 301c-9 through the liquid inlet holes 301b-1 by using a syringe;

injecting a water sample to be detected into the reaction tank 301c-6 through the second reaction liquid inlet through hole 301a-2 and the first reaction liquid inlet through hole 301c-8 in sequence by using a syringe, and inserting the detection card 301 into the accommodating box 302;

the first linear driver 306 is operated to push the injection part 309a into the second reaction liquid inlet through hole 301a-2, the connecting bracket 311 is pushed, the first transmission shaft 319 is inserted into the first knob 301f-1 through the first transmission groove 319a, and the second transmission shaft 318 is inserted into the second knob 301e-2 through the second transmission groove 318 a;

controlling the first transmission motor 304 to act to drive the first microfluidic valve 301f to rotate, so that the first storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first transmission motor 304, and driving the air pump 314 to act to push the reaction reagent in the first storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

controlling the first driving motor 304 to continue to act to drive the first microfluidic valve 301f to continue to rotate, so that the second storage pool 301c-9 is communicated with the reaction pool 301c-6, stopping the action of the first driving motor 304, and driving the air pump 314 to act to push the reaction reagent in the second storage pool 301c-9 into the reaction pool 301c-6, and stopping the action of the air pump 314;

repeating the liquid feeding step to sequentially push the hydroxylamine hydrochloride solution, the sodium citrate solution, the acetic acid-sodium acetate buffer solution and the copper neoxide solution into the reaction tank 301 c-6;

after reacting for 15 minutes in the reaction tank 301c-6, the second transmission motor 317 acts to enable the third liquid outlet channel 301c-1 to be communicated with the fourth liquid outlet channel 301c-5 through the second liquid outlet groove 301e-1, the second transmission motor 317 stops acting, the air pump 314 acts, the mixed solution after the reaction is pushed into the heavy metal detection tank 301c-3, and the redundant mixed solution enters the waste liquid tank 301 c-4;

the light emitting bracket 203 slides outwards to enable the light emitting port 213a to be opposite to the heavy metal detection pool 301c-3, the light emitting lamp tube 214 is electrified, the sleeve 209 rotates to enable the light shielding sheet 211 capable of emitting 457nm wavelength to cover the position of the light emitting port 213a, light is irradiated onto the detector 201 through the heavy metal detection pool 301c-3, the detector 201 detects absorbance to obtain the relationship between the absorbance and the content of heavy metal, the second transmission motor 317 drives the first micro-fluidic valve 301f and the second micro-fluidic valve 301e to rotate back to the initial angle, the pipeline 310 is retracted to the initial position, the third linear driver 313 operates, the movable rod 312 is retracted to the initial position, the third linear driver 313 stops operating, the first transmission shaft 319 and the second transmission shaft 318 leave the detection card 301, the first linear driver 306 operates to enable the push-pull rod to extend outwards, and the pipeline 310 leaves the detection card 301, the first linear actuator 306 stops operating, the second linear actuator 303 operates to extend the lift lever upward, the housing box 302 extends upward out of the connection port 102a, the detection card 301 is taken out, the second linear actuator 303 operates in the reverse direction, the travel lever 312 retracts, the housing box 302 is pulled and retracted, the second linear actuator 303 stops operating, and the detection is completed.

It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.