阅读说明：本技术 样本分析仪及其采样方法 (Sample analyzer and sampling method thereof ) 是由 刘隐明 冯祥 于 2020-06-01 设计创作，主要内容包括：一种样本分析仪及其采样方法,该样本分析仪包括驱动组件、采样组件、反应组件、检测组件、废液处理组件以及控制器；其中,采样组件包括采样针和驱动件,采样针设有采样通道和放气通道,驱动件与采样通道连通,放气通道用于在采样针穿刺试管时将试管内的压力与外界平衡为常压。通过设置采样针设置有采样通道和放气通道,使得采样针在穿刺试管后,试管内的压力通过放气通道与外界平衡为常压,使得驱动件可驱动采样针吸样,样本进入采样通道内,从而实现一次穿刺吸样的目的,加快了样本分析仪的采样速度和测量速度。(A sample analyzer and a sampling method thereof, the sample analyzer comprises a driving component, a sampling component, a reaction component, a detection component, a waste liquid treatment component and a controller; wherein, the sampling subassembly includes sampling needle and driving piece, and the sampling needle is equipped with sampling passageway and air bleed passageway, driving piece and sampling passageway intercommunication, and air bleed passageway is used for balancing the pressure in the test tube with the external world for the ordinary pressure when the test tube is punctured to the sampling needle. Be provided with sampling channel and gassing channel through setting up the sampling needle for the sampling needle is behind the puncture test tube, and the pressure in the test tube is the ordinary pressure through gassing channel and external balance, makes the driving piece can drive the sampling needle and inhale the appearance, and the sample gets into in the sampling channel, thereby realizes the purpose that a puncture was inhaled the appearance, has accelerated sample analyzer's sampling speed and measuring speed.)

1. A sample analyzer is characterized by comprising a driving component, a sampling component, a reaction component, a detection component, a waste liquid treatment component and a controller; the drive assembly is used for driving a flow path in the sample analyzer; the sampling assembly is used for collecting and distributing biological samples; the reaction component is used for processing the biological sample to form a liquid to be detected; the detection assembly is used for detecting the liquid to be detected to form detection information; the waste liquid treatment component is used for collecting and discharging waste liquid in the sample analyzer; the controller is used for controlling the work flow of the sample analyzer and processing the detection information to form an analysis result;

wherein, the sampling subassembly includes sampling needle and driving piece, the sampling needle is equipped with sampling passageway and gassing passageway, the driving piece with the sampling passageway intercommunication, the gassing passageway is used for the pressure in the test tube is the ordinary pressure with external balance when the test tube is punctured to the sampling needle.

2. The sample analyzer of claim 1, wherein the sampling needle comprises a needle body and a needle tip connected with an end of the needle body, the sampling channel and the air release channel are arranged in the needle body and are not communicated with each other at intervals, the sample suction port of the sampling channel and the air suction port of the air release channel are both arranged on the peripheral side wall of the needle body, and the needle tip has a tip end for puncturing the test tube cap.

3. The sample analyzer of claim 2, wherein the aspiration port is disposed further from the needle tip than the sample aspiration port, and the aspiration port has a predetermined distance from a tip end of the needle tip, the predetermined distance being not less than a liquid level of the sample in the test tube.

4. The sample analyzer of claim 2 or 3 wherein the sampling channel is disposed in the middle of the needle body and the deflation channel surrounds the periphery of the sampling channel.

5. The sample analyzer as claimed in claim 1, wherein the sampling assembly further comprises a constant pressure member which is in communication with the outside or has an internal pressure of a constant pressure, and a first switching member for communicating or cutting off the air release passage and the constant pressure member.

6. The sample analyzer of claim 5 wherein the constant pressure element is a swab, the swab having an atmospheric end, the atmospheric end being in communication with the outside, the deflation channel being in communication with the atmospheric end, the communication of the first switch element also being used to vent liquid to the deflation channel.

7. The sample analyzer of claim 6, wherein a first three-way joint is arranged between the first switching member and the atmosphere end, the first three-way joint is further communicated with a first liquid tank, and a second switching member is arranged between the first three-way joint and the first liquid tank.

8. The sample analyzer of claim 7, wherein a second tee joint is provided between the first tee joint and the second switch, the second tee joint further connected to a positive pressure source.

9. The sample analyzer of claim 8, wherein a third switch is provided between the second tee joint and the positive pressure source, the third switch being configured to connect or disconnect the positive pressure source to the second tee joint.

10. The sample analyzer of claim 9 wherein a fourth switch is disposed between the second tee joint and the third switch, the fourth switch being configured to connect or disconnect the second tee joint from the third switch and prevent liquid from flowing from the second tee joint to the third switch.

11. The sample analyzer of claim 9 wherein a diameter of a conduit between the second tee joint and the third switch is smaller than a diameter of other conduits through which liquid of the first liquid tank flows.

12. The sample analyzer of claim 7, wherein a sixth switch is provided between the first three-way joint and the atmospheric air terminal, the sixth switch being configured to connect or disconnect the first three-way joint and the atmospheric air terminal and to block the liquid of the first liquid tank from flowing to the atmospheric air terminal.

13. The sample analyzer of claim 5, wherein the constant pressure element is a constant pressure source, the pressure in the constant pressure source is a constant pressure, and the first switching element is configured to connect or disconnect the air bleeding channel to the constant pressure source.

14. The sample analyzer of claim 13 wherein the sampling assembly further comprises a positive pressure source and a third switch for selectively communicating the positive pressure source or the constant pressure source with the first switch.

15. The sample analyzer of claim 14 wherein a second three-way joint is provided between the vent channel and the first switch, the second three-way joint in turn communicating a second switch with the first liquid tank, the first switch further for preventing liquid from the first liquid tank from flowing into the third switch.

16. The sample analyzer of claim 15 wherein a diameter of a conduit between the second tee fitting and the first switch member is smaller than a diameter of other conduits through which liquid of the first tank flows.

17. The sample analyzer of claim 1 wherein an eighth switch is provided between the sampling channel and the driving member for shutting off the sampling channel from the driving member before the sampling needle pierces a test tube and the pressure in the test tube is not balanced.

18. The sample analyzer of claim 17, wherein the tubing between the sampling channel and the drive member deforms less than a predetermined amount under pressure; or the deformation of the pipeline between the sampling channel and the eighth switching piece under the action of pressure is smaller than a preset value.

19. A sampling method of a sample analyzer, wherein a sampling assembly of the sample analyzer comprises a sampling needle and a driving member, the sampling needle is provided with a sampling channel and a gas release channel, and the driving member is communicated with the sampling channel, the sampling method comprises the following steps:

the sampling needle punctures the test tube, and the air discharge channel is communicated with the outside, so that the pressure in the test tube is balanced with the outside to be normal pressure;

and when the pressure in the test tube is normal pressure, the driving piece drives the sampling channel of the sampling needle to suck the sample in the test tube.

20. The sampling method of claim 19, wherein the sampling assembly comprises a constant pressure member and a first switching member, the constant pressure member is in communication with the outside or the internal pressure is normal pressure, and the air bleeding passage is in communication with the outside comprises: the air discharge channel is communicated with the normal pressure piece, and the first switching piece is communicated with or cuts off the air discharge channel and the normal pressure piece.

21. The sampling method of claim 20, wherein the constant pressure component is a swab, the swab is provided with an atmospheric end, the atmospheric end is in communication with the outside, and the deflation channel is in communication with the atmospheric end, the sampling method comprising:

the air discharge channel is communicated with the air at the atmosphere end, so that the pressure in the test tube is balanced with the external pressure and reaches the normal pressure.

22. A sampling method according to claim 21, wherein after said sampling needle has completed its aspiration and has exited the tube, said sampling method further comprises the step of washing said sampling needle, said first switch being in communication to pass liquid to said deflation path for washing.

23. The sampling method according to claim 22, wherein a first three-way joint is provided between the first switching member and the atmosphere end, the first three-way joint is further communicated with a first tank, and a second switching member is provided between the first three-way joint and the first tank, the sampling method comprising:

the second switching piece is communicated with the first liquid tank and the first three-way piece, and liquid in the first liquid tank flows into the atmosphere end of the swab through the first three-way piece so as to clean the outer wall of the sampling needle.

24. The sampling method of claim 23, wherein the method further comprises:

the first switching piece is communicated with the air bleeding channel and the first three-way piece, and liquid in the first liquid tank flows into the air bleeding channel through the first three-way piece so as to clean the inner wall of the air bleeding channel.

25. The sampling method of claim 22, further comprising:

and after the sampling needle completes sample division, the driving piece is introduced with liquid to clean the sampling channel of the sampling needle.

26. The sampling method of claim 24, wherein a second tee joint is provided between the first tee joint and the second switch, the second tee joint is connected to a positive pressure source, a third switch is provided between the second tee joint and the positive pressure source, and when the deflation channel of the sampling needle is cleaned, the sampling method further comprises the step of drying the deflation channel, comprising:

the third switching piece is communicated with the positive pressure source and the second three-way joint, the first switching piece is communicated with the deflation channel and the first three-way joint, and gas of the positive pressure source enters the deflation channel through the third switching piece, the second three-way joint, the first three-way joint and the first switching piece.

27. The sampling method of claim 26, wherein a fourth switch is disposed between the second tee fitting and the third switch, the sampling method further comprising:

when the step of cleaning the sampling needle is performed, the fourth switching piece cuts off the second three-way joint and the third switching piece, and liquid of the first liquid tank is prevented from flowing into the third switching piece;

when the step of drying the air discharging channel is performed, the fourth switching piece is communicated with the second three-way joint and the third switching piece.

28. The sampling method of claim 26, wherein a diameter of a pipe between the second three-way joint and the third switch is smaller than diameters of other pipes through which liquid of the first liquid tank flows, the sampling method further comprising:

when the step of cleaning the sampling needle is performed, the liquid of the first liquid tank flows into the atmosphere end of the first switch piece and/or the swab at the second three-way joint;

when the step of drying the deflation channel is performed, the flow speed of the gas of the positive pressure source in the pipeline between the second three-way joint and the third switching piece is faster, so that the drying speed is accelerated.

29. The sampling method of claim 23, wherein a sixth switch is provided between the first three-way joint and the atmospheric end, the sampling method further comprising:

when the sampling needle exits the test tube, the sixth switching piece cuts off the first three-way joint and the atmosphere end, so that the liquid of the first liquid tank flows into the air release channel and does not flow into the atmosphere end.

30. The sampling method of claim 20, wherein the constant pressure component is a constant pressure source and the pressure within the constant pressure source is a constant pressure, the sampling method comprising:

when the sampling needle penetrates into the test tube, the first switching piece is communicated with the air release channel and the normal pressure source, and the air release channel achieves pressure balance through air circulation with the normal pressure source.

31. The sampling method of claim 30, further comprising a positive voltage source and a third switch, the sampling method further comprising:

after the sampling needle penetrates into the test tube, the third switching piece is communicated with the normal pressure source and the first switching piece;

and after the sampling needle is cleaned, the third switching piece is communicated with the positive pressure source and the first switching piece.

32. The sampling method of claim 31, wherein a second three-way joint is provided between the air bleed passage and the first switch, the second three-way joint communicating, in turn, a second switch and a first liquid tank, the sampling method further comprising:

when the sampling needle is cleaned, the first switching piece cuts off the third switching piece and the second three-way joint, and the liquid of the first liquid tank flows into the air bleeding channel through the second switching piece and the second three-way joint.

33. The sampling method of claim 19, wherein an eighth switch is provided between the sampling channel and the drive member, the sampling method further comprising:

when the sampling needle pierces the test tube and the pressure in the test tube is not balanced, the eighth switching member cuts off the sampling channel and the driving member.

Technical Field

The invention relates to the technical field of medical instruments, in particular to a sample analyzer and a sampling method thereof.

Background

The sample analyzer needs to extract the biological sample from the closed test tube for analysis, and because the pressure generally exists in the closed test tube, the pressure can produce adverse effect to the sampling accuracy, so how to solve the problem that accurate sampling from the closed test tube is generally faced in the industry.

The current scheme usually adopts two punctures, the first puncture of sampling needle is puncture preliminary treatment to release the pressure in the test tube, then wash the sampling needle, and then the sampling needle carries out the second puncture in order to absorb the biological sample. Because the sampling needle needs to puncture twice and needs to be cleaned in the middle of the two punctures, the sampling speed of the sample analyzer is directly limited, and the measurement speed of the sample analyzer is slow.

Disclosure of Invention

In view of the above, an object of the present invention is to provide a sample analyzer and a sampling method thereof, which can perform sampling by one puncture.

In order to realize the purpose of the invention, the invention provides the following technical scheme:

in a first aspect, the present invention provides a sample analyzer, comprising a driving component, a sampling component, a reaction component, a detection component, a waste liquid treatment component and a controller; the drive assembly is used for driving a flow path in the sample analyzer; the sampling assembly is used for collecting and distributing biological samples; the reaction component is used for processing the biological sample to form a liquid to be detected; the detection assembly is used for detecting the liquid to be detected to form detection information; the waste liquid treatment component is used for collecting and discharging waste liquid in the sample analyzer; the controller is used for controlling the work flow of the sample analyzer and processing the detection information to form an analysis result;

wherein, the sampling subassembly includes sampling needle and driving piece, the sampling needle is equipped with sampling passageway and gassing passageway, the driving piece with the sampling passageway intercommunication, the gassing passageway is used for the pressure in the test tube is the ordinary pressure with external balance when the test tube is punctured to the sampling needle.

Wherein, the sampling needle include the needle body and with needle body end connection's needle point, the sampling passageway with the gassing passageway set up in the needle is internal, just the sampling passageway with the gassing passageway interval sets up and does not communicate, the appearance mouth of inhaling of sampling passageway with the induction port of gassing passageway all locates on the peripheral lateral wall of needle body, the needle point has the pointed end and is used for puncturing the test tube cap.

The aspiration port is arranged at a position which is farther away from the needle tip than the sample suction port, a preset distance is formed between the aspiration port and the tip end of the needle tip, and the preset distance is not less than the liquid level height of a sample in the test tube.

Wherein, the sampling channel is arranged in the middle of the needle body, and the air release channel surrounds the periphery of the sampling channel.

The sample analyzer further comprises a constant pressure piece and a first switching piece, the constant pressure piece is communicated with the outside or the internal pressure is normal pressure, the air discharging channel is communicated with the constant pressure piece, and the first switching piece is used for communicating or cutting off the air discharging channel and the constant pressure piece.

The normal-pressure piece is a swab, the swab is provided with an atmosphere end, the atmosphere end is communicated with the outside, the air discharge channel is communicated with the atmosphere end, and the communication of the first switching piece is also used for introducing liquid into the air discharge channel.

The first three-way joint is communicated with a first liquid tank, and a second switching piece is arranged between the first three-way joint and the first liquid tank.

And a second three-way joint is arranged between the first three-way joint and the second switching piece, and the second three-way joint is also connected with a positive pressure source.

And a third switching piece is arranged between the second three-way joint and the positive pressure source and is used for communicating or cutting off the positive pressure source and the second three-way joint.

And a fourth switching piece is arranged between the second three-way joint and the third switching piece, and is used for communicating or cutting off the second three-way joint and the third switching piece and preventing liquid from flowing from the second three-way joint to the third switching piece.

Wherein a diameter of a pipe between the second three-way joint and the third switching member is smaller than a diameter of other pipes through which the liquid of the first liquid tank flows.

And a sixth switching piece is arranged between the first three-way joint and the atmosphere end and is used for communicating or cutting off the first three-way joint and the atmosphere end and preventing the liquid of the first liquid tank from flowing to the atmosphere end.

The constant pressure component is a constant pressure source, the pressure in the constant pressure source is constant pressure, and the first switching component is used for communicating or cutting off the air release channel and the constant pressure source.

Wherein the sampling assembly further comprises a positive pressure source and a third switch for selectively communicating either the positive pressure source or the constant pressure source with the first switch.

The first switching piece is used for preventing liquid in the first liquid tank from flowing into the second switching piece.

Wherein a diameter of a pipe between the second three-way joint and the first switching member is smaller than a diameter of other pipes through which liquid of the first liquid tank flows.

The sampling channel and the driving piece are provided with an eighth switching piece, the sampling needle punctures the test tube, and before the pressure in the test tube is unbalanced, the eighth switching piece is used for cutting off the sampling channel and the driving piece.

The deformation of a pipeline between the sampling channel and the driving piece under the action of pressure is smaller than a preset value; or the deformation of the pipeline between the sampling channel and the eighth switching piece under the action of pressure is smaller than a preset value.

In a second aspect, the present invention further provides a sampling method of a sample analyzer, in which a sampling assembly of the sample analyzer includes a sampling needle and a driving member, the sampling needle is provided with a sampling channel and a gas release channel, and the driving member is communicated with the sampling channel, and the sampling method includes:

the sampling needle punctures the test tube, and the air discharge channel is communicated with the outside, so that the pressure in the test tube is balanced with the outside to be normal pressure;

and when the pressure in the test tube is normal pressure, the driving piece drives the sampling channel of the sampling needle to suck the sample in the test tube.

Wherein, the sampling subassembly includes constant pressure spare and first switching element, constant pressure spare and external intercommunication or internal pressure are the ordinary pressure, the gassing passageway includes with external intercommunication: the air discharge channel is communicated with the normal pressure piece, and the first switching piece is communicated with or cuts off the air discharge channel and the normal pressure piece.

The sampling method comprises the following steps of:

the air discharge channel is communicated with the air at the atmosphere end, so that the pressure in the test tube is balanced with the external pressure and reaches the normal pressure.

When the sampling needle finishes sampling and withdraws from the test tube, the sampling method further comprises the step of cleaning the sampling needle, and the first switching piece is communicated to introduce liquid into the air bleeding channel for cleaning.

The sampling method comprises the following steps that a first three-way joint is arranged between the first switching piece and the atmosphere end, the first three-way joint is further communicated with a first liquid tank, a second switching piece is arranged between the first three-way joint and the first liquid tank, and the sampling method comprises the following steps:

the second switching piece is communicated with the first liquid tank and the first three-way piece, and liquid in the first liquid tank flows into the atmosphere end of the swab through the first three-way piece so as to clean the outer wall of the sampling needle.

Wherein the method further comprises:

the first switching piece is communicated with the air bleeding channel and the first three-way piece, and liquid in the first liquid tank flows into the air bleeding channel through the first three-way piece so as to clean the inner wall of the air bleeding channel.

Wherein, still include:

and after the sampling needle completes sample division, the driving piece is introduced with liquid to clean the sampling channel of the sampling needle.

When the deflation channel of the sampling needle is cleaned, the sampling method further comprises a step of drying the deflation channel, and the sampling method comprises the following steps:

the third switching piece is communicated with the positive pressure source and the second three-way joint, the first switching piece is communicated with the deflation channel and the first three-way joint, and gas of the positive pressure source enters the deflation channel through the third switching piece, the second three-way joint, the first three-way joint and the first switching piece.

Wherein a fourth switching piece is arranged between the second three-way joint and the third switching piece, and the sampling method further comprises the following steps:

when the step of cleaning the sampling needle is performed, the fourth switching piece cuts off the second three-way joint and the third switching piece, and liquid of the first liquid tank is prevented from flowing into the third switching piece;

when the step of drying the air discharging channel is performed, the fourth switching piece is communicated with the second three-way joint and the third switching piece.

Wherein a diameter of a pipe between the second three-way joint and the third switching member is smaller than diameters of other pipes through which the liquid of the first liquid tank flows, the sampling method further comprising:

when the step of cleaning the sampling needle is performed, the liquid of the first liquid tank flows into the atmosphere end of the first switch piece and/or the swab at the second three-way joint;

when the step of drying the deflation channel is performed, the flow speed of the gas of the positive pressure source in the pipeline between the second three-way joint and the third switching piece is faster, so that the drying speed is accelerated.

Wherein a sixth switching piece is arranged between the first three-way joint and the atmosphere end, and the sampling method further comprises the following steps:

when the sampling needle exits the test tube, the sixth switching piece cuts off the first three-way joint and the atmosphere end, so that the liquid of the first liquid tank flows into the air release channel and does not flow into the atmosphere end.

The sampling method comprises the following steps of:

when the sampling needle penetrates into the test tube, the first switching piece is communicated with the air release channel and the normal pressure source, and the air release channel achieves pressure balance through air circulation with the normal pressure source.

The sampling method further comprises the following steps of:

after the sampling needle penetrates into the test tube, the third switching piece is communicated with the normal pressure source and the first switching piece;

and after the sampling needle is cleaned, the third switching piece is communicated with the positive pressure source and the first switching piece.

Wherein, be equipped with second three way connection between gassing passageway and the first switching piece, second three way connection communicates second switching piece and first fluid reservoir in proper order, the sampling method still includes:

when the sampling needle is cleaned, the first switching piece cuts off the third switching piece and the second three-way joint, and the liquid of the first liquid tank flows into the air bleeding channel through the second switching piece and the second three-way joint.

Wherein, be equipped with the eighth switching piece between sampling channel and the driving piece, the sampling method still includes:

when the sampling needle pierces the test tube and the pressure in the test tube is not balanced, the eighth switching member cuts off the sampling channel and the driving member.

According to the sample analyzer provided by the invention, the sampling needle is provided with the sampling channel and the air release channel, so that after the sampling needle punctures the test tube, the pressure in the test tube is balanced with the outside to be normal pressure through the air release channel, the driving piece can drive the sampling needle to suck a sample, and the sample enters the sampling channel, thereby achieving the purpose of puncturing and sucking the sample at one time and accelerating the sampling speed and the measuring speed of the sample analyzer.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a cross-sectional structural schematic view of a vertical cross-section of a sampling needle of an embodiment;

FIG. 2 is a cross-sectional structural schematic view of a vertical cross-section of a sampling needle of an embodiment;

FIG. 3 is a cross-sectional structural schematic view of a cross-section of one embodiment of a sampling needle;

FIG. 4 is a schematic view of a sampling assembly of the sample analyzer of an embodiment;

FIG. 5 is a schematic view of a sampling assembly of the sample analyzer of an embodiment;

FIG. 6 is a schematic view of a sampling assembly of an embodiment of a sample analyzer;

FIG. 7 is a schematic view of a sampling assembly of an embodiment of a sample analyzer;

FIG. 8 is a schematic view of a sampling assembly of the sample analyzer of an embodiment;

FIG. 9 is a schematic diagram of a sampling assembly of the sample analyzer of FIG. 8 in use;

FIG. 10 is a schematic view of a sampling assembly of the sample analyzer of FIG. 8 in use;

FIG. 11 is a schematic diagram of a sampling assembly of the sample analyzer of FIG. 8 in use.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

The embodiment of the invention provides a sample analyzer. The sample analyzer may be used to perform analysis of biological samples, which may be blood, urine, etc. The sample analyzer includes a sampling assembly for collecting and dispensing a biological sample. The sample analyzer further comprises a driving assembly, a reaction assembly, a detection assembly, a waste liquid treatment assembly and a controller. The drive assembly is used to drive various flow paths (including gas and liquid paths) in the sample analyzer. The reaction component is used for processing the biological sample to form a liquid to be detected. The detection component is used for detecting the liquid to be detected to form detection information. The waste liquid treatment assembly is used for collecting and discharging waste liquid in the sample analyzer. The controller is used for controlling the work flow of the sample analyzer and processing the detection information to form an analysis result.

Referring to fig. 1 and 4, the sampling assembly includes a sampling needle 1 and a driving member 2, the sampling needle 1 is provided with a sampling channel 103 and an air release channel 104, the driving member 2 is communicated with the sampling channel 103, and the air release channel 104 is used for balancing the pressure in the test tube with the outside to be normal pressure when the sampling needle punctures the test tube.

In this embodiment, include sampling channel 103 and air bleed channel 104 through setting up sampling needle 1 for sampling needle 1 is after the puncture test tube, and the pressure in the test tube is the ordinary pressure through air bleed channel 104 and external balance, makes driving piece 2 can drive sampling needle 1 and inhale the appearance, and the sample gets into in the sampling channel 103, thereby realizes the purpose that the appearance was inhaled in the puncture once, has accelerated sample analyzer's sampling speed and measuring speed.

In one embodiment, referring to fig. 1, the sampling needle 1 includes a needle body 101 and a needle tip 102 connected to an end of the needle body 101, a sampling channel 103 and a deflation channel 104 are disposed inside the needle body 102, the sampling channel 103 and the deflation channel 104 are disposed at intervals and are not communicated, and the needle tip 102 has a tip end for puncturing a test tube cap. The sampling channel 103 has a sample suction port 105, the air bleeding channel 104 has a suction port 106, and the sample suction port 105 and the suction port 106 are provided on the outer peripheral side wall of the needle body 101.

In this embodiment, the specific shape and size of the sampling channel 103 and the air bleeding channel 104 are not limited, and it is preferable that the cross-section of the sampling channel 103 and the air bleeding channel 104 is circular, and the diameter of the air bleeding channel 104 is larger than that of the sampling channel 103, so that the air flow speed between the inside of the test tube and the outside is increased, and the balance is achieved more quickly, so as to increase the sampling speed.

In this embodiment, the sample suction port 105 and the suction port 106 can be disposed at the same distance from the needle tip 102 and as close to the needle tip 102 as possible, for example, the sample suction port 105 and the suction port 106 are located 0.5mm to 5mm from the end surface of the needle tip 102. It will be appreciated that the sample aspiration port 105 and aspiration port 106 may have a small deviation from the distance of the needle tip 102, for example, the sample aspiration port 105 and aspiration port 106 may have a deviation from the distance of the needle tip 102 of 0.1mm to 5 mm. In this embodiment, when the sampling needle 1 penetrates into the test tube and is not immersed into the sample (it should be understood that the needle tip 102 may partially enter the sample, but the air suction port 106 should be avoided to enter the sample), the sampling needle 1 may be further inserted after the pressure in the test tube is balanced by the air suction port 106 and the air release channel 104, and the sample suction port 105 is immersed into the sample for sample suction.

In another embodiment, referring to fig. 2, the sample suction port 105 and the aspiration port 106 are located at different distances from the tip 102, and the aspiration port 106 is located at a predetermined distance away from the tip of the tip 102, wherein the predetermined distance is not less than the liquid level of the sample in the test tube. After the sampling needle 1 of the present embodiment is inserted into a test tube, the sample suction port 105 is immersed into the sample, and the air suction port 106 is located above the liquid level of the sample, so that the air circulation and the pressure balance between the inside of the test tube and the outside are maintained before and after the sample is sucked by the sample suction port 105. The sampling needle 1 of this embodiment need not pause and wait after the puncture gets into the test tube, has saved the time of gassing, is favorable to accelerating the sampling speed.

In one embodiment, referring to fig. 3, the sampling channel 103 of the sampling needle 1 is disposed in the middle of the needle body 101, and the air release channel 104 surrounds the outer periphery of the sampling channel 103. Preferably, the sampling channel 103 and the deflation channel 104 form a concentric circular configuration. In this embodiment, the sample suction port 105 (refer to fig. 2) of the sampling channel 103 is located closer to the needle tip 102 than the suction port 106, so that the sample suction port 105 does not intersect the air release channel 104 when it is led out from the middle of the needle body 101 to the outer wall of the needle body 101.

In one embodiment, the sampling channel and the deflation channel can be respectively provided with two sub-sampling needles, the two sub-sampling needles are arranged side by side, only one channel is arranged in each sub-sampling needle, one sub-sampling needle is used for sample suction and sample division, and the other sub-sampling needle is used for deflation.

In one embodiment, referring to fig. 4 in combination with fig. 1, the sampling assembly further includes a swab 3, the swab 3 having an atmospheric end, and the deflation channel 104 of the sampling needle 1 is connected to the atmospheric end of the swab 3. After the sampling needle 1 punctures the test tube, the gas in the test tube circulates with the outside through the atmosphere end of the swab 3, and the pressure in the test tube is balanced with the outside.

In this embodiment, the swab 3 is used to clean the outer wall of the sampling needle 1 and recover waste liquid after cleaning, and during cleaning, the atmospheric end of the swab 3 is filled with liquid and sprayed to the outer wall of the sampling needle 1, so as to achieve the purpose of cleaning. Because the washing of the outer wall of the sampling needle 1 is not performed simultaneously with the puncture of the test tube, the atmosphere end of the swab 3 is communicated with the outside when the test tube is punctured. Thus, the present embodiment communicates the deflation channel 104 to the atmosphere end of the swab 3, enabling functional reuse of the swab 3.

In this embodiment, a first switch 11 is further disposed between the deflation channel 104 of the sampling needle 1 and the atmosphere end of the swab 3, and the first switch 11 is used for connecting or disconnecting the deflation channel 104 and the atmosphere end of the swab 3. Furthermore, since the atmosphere end of the swab 3 will receive the liquid for washing the outer wall of the sampling needle 1, and the atmosphere end of the swab 3 communicates with the deflation channel 104, the liquid can also flow into the deflation channel 104 for washing, and therefore the communication of the first switch 11 also serves to access the liquid for washing the inner wall of the deflation channel 104 of the sampling needle 1. The first switch member 1 may be a solenoid valve.

Further, a first three-way joint 12 is provided between the first switching element 11 and the atmosphere end of the swab 3, the first three-way joint 12 is further communicated with a first liquid tank 15, and a second switching element 14 is further provided between the first three-way joint 12 and the first liquid tank 15. The first liquid tank 15 is used to supply liquid, which may be diluent, for cleaning the outer wall of the sampling needle 1 or the inner wall of the deflation channel 104. The three joints of the first three-way joint 12 are communicated in pairs, so that the liquid of the first liquid tank 15 can flow to the atmosphere end of the swab 3 and can also flow to the air release channel 104 of the sampling needle 1 through the first switching element 11. The second switching member 14 is used for communicating the first three-way joint 12 with the first liquid tank 15 to supply liquid for cleaning the sampling needle 1. The second switching member 14 may be a solenoid valve.

When the sampling needle 1 pierces the test tube and the first switch 11 communicates with the air release channel 104 to release air through the first three-way joint 12 and the atmosphere end of the swab 3, the air release channel 104 or the outer wall of the sampling needle 1 is not cleaned, and the second switch 14 cuts off the first three-way joint 12 and the first liquid tank 15 at this time.

After the sampling needle 1 finishes withdrawing the test tube after sucking the sample, the outer wall of the sampling needle 1 needs to be cleaned, at this time, the first switching piece 11 cuts off the deflation channel 104 and the first three-way joint 12, the second switching piece 14 is communicated with the first three-way joint 12 and the first liquid tank 15, and the liquid flows to the atmosphere end of the swab 3 to clean the outer wall of the sampling needle 1.

After the outer wall of the sampling needle 1 is cleaned, the sampling needle 1 performs sample separation, and after the sample separation is completed, the outer walls of the gas discharge channel 104 and the sampling needle 1 need to be cleaned, at this time, the first switching piece 11 is communicated with the sampling channel 104 and the first three-way joint 12 of the sampling needle 1, the second switching piece 14 is communicated with the first three-way joint 12 and the first liquid tank 15, so that liquid in the first liquid tank 15 flows into the gas discharge channel 104 of the sampling needle 1 through the second switching piece 14, the first three-way joint 12 and the first switching piece 11, and part of liquid also flows to the atmosphere end of the swab 3 at the first three-way joint 12, thereby realizing the function of cleaning the gas discharge channel 103 and the outer wall of the sampling needle 1 at the same time.

The swab 3 is also provided with a recovery end which is communicated with a waste liquid tank 31, when the swab 3 is cleaned on the outer wall of the sampling needle 1, the recovery end collects the cleaned waste liquid, and the waste liquid flows to the waste liquid tank 31 for storage. The recovery end of the swab 3 may also collect waste fluid when the deflation channel 104 of the sampling needle 1 is cleaned.

In one embodiment, a second three-way joint 13 is disposed between the first three-way joint 12 and the second switching member 14, and the second three-way joint 13 is further connected to a positive pressure source 18. The positive pressure source 18 is used to blow air to dry the deflation channel 104.

In this embodiment, a third switching member 17 is provided between the second three-way joint 13 and the positive pressure source 18, and the third switching member 17 is used to connect or disconnect the positive pressure source 18 and the second three-way joint 13. The third switching member 17 may be a solenoid valve.

After the air discharge channel 104 and the outer wall of the sampling needle 1 are cleaned, the second switching piece 14 disconnects the first three-way joint 12 from the first liquid tank 15, and the step of drying the air discharge channel 104 is started. Specifically, the third switching member 17 communicates the positive pressure source 18 with the second three-way joint 13, and the first switching member 11 communicates the first three-way joint 12 with the air bleeding passage 104 of the sampling needle 1. The gas of the positive pressure source 18 enters the deflation channel 104 of the sampling needle 1 through the third switching piece 17, the second three-way joint 13, the first three-way joint 12 and the first switching piece 11, the deflation channel 104 is dried, then the first switching piece 11 and the third switching piece 17 are closed, and the supply of the gas of the positive pressure source 17 is cut off.

When the positive pressure source 18 blows air into the deflation channel 104 of the sampling needle 1, part of the air flows through the first three-way connection 12 to the atmosphere end of the swab 3, blowing air into the outer wall of the sampling needle 1 and accelerating the drying of the outer wall of the sampling needle 1.

Preferably, a fourth switching member 16 is disposed between the second three-way joint 13 and the third switching member 17, the fourth switching member 16 is used for connecting or disconnecting the third switching member 17 and the second three-way joint 13, and when the fourth switching member 16 is connected to the third switching member 17 and the second three-way joint 13, the liquid can be prevented from flowing from the second three-way joint 13 to the third switching member 17. When the air release channel 104 and the outer wall of the sampling needle 1 are cleaned, the liquid in the first liquid tank 15 flows to the second three-way joint 13, and in order to prevent the liquid from flowing to the third switching piece 17 and causing pollution, the fourth switching piece 16 is arranged for blocking. The fourth switch 16 may be a check valve.

In one embodiment, referring to fig. 1 and 4, the driving member 2 drives the sampling needle 1 to suck and divide the sample, and when the division is completed, the driving member 2 is further used for supplying liquid to clean the sampling channel 103 of the sampling needle 1. The drive member 2 may be a syringe or a metered dose pump.

Preferably, the driving member 2 is connected to a second liquid tank 22, and a cleaning liquid is stored in the second liquid tank 22 and is used for supplying liquid to the driving member 2 to clean the sampling passage 103 of the sampling needle 1.

Further, a fifth switching member 21 is disposed between the second tank 22 and the driving member 2, and the fifth switching member 21 is used for connecting or disconnecting the second tank 22 and the driving member 2. When the sampling needle 1 punctures the test tube for sampling, the fifth switching piece 21 cuts off the second liquid tank 22 and the driving piece 2; after sample division is finished, the fifth switching piece 21 is communicated with the second liquid tank 22 and the driving piece 2, the driving piece 2 supplies liquid to the sampling needle 1, and the sampling channel 103 of the sampling needle 1 is cleaned; after the sampling passage 103 is cleaned, the driving member 2 returns to the original position, and the fifth switching member 21 disconnects the second tank 22 from the driving member 2.

It should be understood that the above-mentioned communication between the components means communication through a pipe.

In one embodiment, referring to fig. 5 in combination with fig. 1, the diameter of the pipe between the second three-way joint 13 and the third switching member 17 is smaller than the diameter of the other pipe through which the liquid of the first liquid tank 15 flows. Specifically, the pipes through which the liquid of the first liquid tank 15 flows are: 1) a pipe from the first liquid tank 15 to the air bleeding channel 104 of the sampling needle 1 via the second switching member 14, the second three-way joint 13, the first three-way joint 12, the first switching member 11, and the first three-way joint 12 to the atmosphere end of the swab 3 in this order.

In this embodiment, the diameter of the pipe between the second three-way joint 13 and the third switch 17 is small, so that most of the liquid flowing from the second switch 14 to the second three-way joint 13 flows away from the pipe with a larger diameter (i.e., the pipe from the second three-way joint 13 to the first switch 11 via the first three-way joint 12 and the pipe from the first three-way joint 12 to the atmosphere end of the swab 3), thereby reducing the liquid flowing from the second three-way joint 13 into the third switch 13 and preventing the third switch 17 from being easily contaminated. The pipe between the second three-way joint 13 and the third switching piece 17 is preferably a rubber pipe, and the other pipes may be steel pipes.

In this embodiment, the diameter of the pipe between the second three-way joint 13 and the third switching member 17 is set to be smaller, so that the fourth switching member 16 (see fig. 4) may not be provided, and the cost is saved.

In an embodiment, referring to fig. 6 in combination with fig. 1, a sixth switch 32 is disposed between the first three-way joint 12 and the atmosphere end of the swab 3, and the sixth switch 32 is used for connecting or disconnecting the first three-way joint 12 and the atmosphere end of the swab 3.

In this embodiment, when the deflation channel 104 of the sampling needle 1 is cleaned, the sixth switch 32 can cut off the communication between the first three-way joint 12 and the atmosphere end of the swab 3, so that the liquid in the first liquid tank 15 does not flow to the atmosphere end of the swab 3, that is, when the deflation channel of the sampling needle 1 is cleaned, the outer wall of the sampling needle 1 is not cleaned simultaneously, the liquid consumption of the first liquid tank 15 can be reduced, the amount of waste liquid recovered by the swab 3 is reduced, and the cleaning time is shortened.

In an embodiment, referring to fig. 7 in combination with fig. 1, unlike the previous embodiment, the deflation channel 104 of the sampling needle 1 of the present embodiment is connected to the normal pressure source 19, and the first switch 11 is used for connecting or disconnecting the deflation channel 104 of the sampling needle 1 and the normal pressure source 19.

In this embodiment, the air release channel 104 of the sampling needle 1 is communicated to the normal pressure source 19, so that after the sampling needle 1 is punctured and enters the test tube, the first switching member 11 is communicated with the air release channel 104 of the sampling needle 1 and the normal pressure source 19, air in the test tube flows with the normal pressure source 19, pressure balance is achieved, the pressure in the test tube is normal pressure, sample suction can be performed, and the purpose of sample suction through one puncture is achieved.

In this embodiment, the normal pressure source 19 may be connected to the third switch 17, the first switch 11 may be connected to the third switch 17, the third switch 17 may be connected to the positive pressure source 18, and the third switch 17 may be used to selectively connect the positive pressure source 18 or the normal pressure source 19 to the first switch 11. The first switching member 11 may be a solenoid valve or a check valve, and the third switching member 17 may be a two-position three-way solenoid valve.

When the sampling needle 1 pierces the test tube and needs to be deflated, the third switch 17 communicates the constant pressure source 19 with the first switch 11. When the sampling needle 1 needs to blow dry the deflation path, the third switch 17 connects the positive pressure source 18 to the first switch 11.

Further, a second three-way joint 13 is provided between the air bleeding channel 104 of the sampling needle 1 and the first switching member 11, the second three-way joint 13 is communicated with a second switching member 14, and the second switching member 14 is communicated with the first liquid tank 15. The first switching member 11 also functions to block the liquid of the first liquid tank 15 from flowing into the third switching member 17.

Preferably, the diameter of the pipe between the second three-way joint 13 and the first switch member 11 is smaller than the diameter of the other pipe through which the liquid of the first liquid tank 15 flows. Specifically, the other pipes through which the liquid 15 of the first liquid tank 15 flows are: the first liquid tank 15 is connected to the air discharge channel of the sampling needle 1 through the second switching piece 14 and the second three-way joint 13.

Further, the atmosphere end of the swab 3 is communicated with a third liquid tank 33, and the third liquid tank 33 is used for supplying liquid to the atmosphere end of the swab 3 so as to clean the outer wall of the sampling needle 1.

Preferably, a seventh switching member 34 is provided between the third liquid tank 33 and the atmosphere end of the swab 3, and the seventh switching member 34 is used to connect or disconnect the third liquid tank 33 and the atmosphere end of the swab 3.

After the sampling needle 1 finishes withdrawing the test tube after absorbing the sample, the seventh switching element 34 communicates the third liquid tank 33 with the atmosphere end of the swab 3, and liquid is introduced into the atmosphere end of the swab 3 to clean the outer wall of the sampling needle 1.

In one embodiment, referring to fig. 8 in combination with fig. 1, an eighth switch 4 is disposed between the sampling channel 103 of the sampling needle 1 and the driving member 2, and the eighth switch 4 is used for connecting or disconnecting the sampling channel 103 and the driving member 2. The eighth switching piece 4 may be a solenoid valve.

When the sampling needle 1 is inserted into a test tube without being immersed in the sample, the pressure in the test tube may be relatively high, and a part of the pressure is communicated with the outside through the air release passage 104. Because the time is needed for the pressure in the test tube to form the normal pressure, and partial pressure in the test tube acts on the sampling channel 103, the volume of the isolation gas column established in the sampling channel 103 changes, which may cause the change of the sample suction amount of the sampling needle 1, and the sample separation liquid is insufficient. Therefore, before the in-tube pressure is not balanced yet, eighth switching member 4 is provided to cut off sampling passage 103 and driving member 2, reducing the influence of the in-tube pressure on sampling passage 103.

Referring to fig. 2 or fig. 3, when the air suction port 106 of the air discharge channel 104 of the sampling needle 1 is farther from the needle tip 102 than the sample suction port 105 of the sampling channel 103, since the sample suction port 105 penetrates through the test tube cap and enters the test tube when the sampling needle 1 pierces the test tube, the air discharge channel 104 does not enter the test tube yet, and when the sampling needle 1 is used for sucking the sample, the air suction port 106 enters the test tube, and the air suction port 106 is always located on the liquid level of the sample. Set up eighth switch 4 and cut off sampling passageway 103 and driving piece 2 when sampling needle 1 punctures, can also avoid not getting into the test tube when induction port 106, and in the test tube for the malleation and impress the sample in the test tube into sampling passageway 103, cause the isolation gas column to shrink, the condition emergence of the accuracy variation of sampling.

In one embodiment, the air discharge channel of the sampling needle 1 can also be an air discharge groove arranged on the outer surface of the sampling needle 1, so that the air discharge channel does not need to be manufactured in the sampling needle 1, and the process is simpler.

Specifically, the air release groove is arranged along the extending direction of the sampling needle 1, and after the sampling needle 1 punctures and enters the test tube, air in the test tube circulates outside the test tube through the air release groove, so that the normal pressure is achieved and the balance is realized.

In one embodiment, the deformation of the pipeline between the sampling channel 103 of the sampling needle 1 and the driving member 2 under the action of pressure is smaller than a preset value, so that the influence of the pressure in the test tube on the volume change of the isolation gas column is ignored after the sampling needle 1 punctures the test tube.

In this embodiment, the preset value may be, for example, 0.01ul/kpa, and the pipe between the sampling channel 103 of the sampling needle 1 and the driving member 2 may be deformed by selecting a material to be less than the preset value, for example, selecting a material with a higher hardness, such as a steel pipe.

Preferably, only the deformation amount of the pipe between sampling channel 103 and eighth switch 4 under pressure may be set to be smaller than a preset value. Since the deformation of the pipeline is related to the material of the pipeline and the length of the pipeline, the deformation of the pipeline between the sampling channel 103 and the eighth switching member 4 is set to be smaller than the preset value, which is easier to realize compared with the whole pipeline (i.e. the pipeline between the sampling channel 103 and the driving member 2), and the cost can be reduced when the same material is made.

When the sampling unit of the sample analyzer of this embodiment is used, referring to fig. 9, before the sampling needle 1 pierces the test tube, the eighth switch 4 is closed, the sampling needle 1 and the driving member 2 are cut off, and then the piercing is performed. Referring to fig. 10, the sampling needle 1 is inserted into the test tube 9, and the pressure in the test tube 9 is released through the air release channel or air release groove of the sampling needle 1. Referring to fig. 11, when the pressure in the test tube 9 is balanced with the outside and reaches the normal pressure, the eighth switch 4 is opened to communicate the sampling needle 1 and the driving member 2, and the driving member 2 drives the sampling needle 1 to suck the sample, thereby completing the process of one-time puncturing and sample sucking.

Through tests, when the sampling assembly of the sample analyzer in the embodiment is used, the volume variation range of the isolated gas column is less than +/-0.3 ul, while the volume of the isolated gas column established before puncturing is more than 2ul, and the variation is completely acceptable.

Referring to fig. 1 and 4, a sampling assembly of the sample analyzer includes a sampling needle 1 and a driving member 2, the sampling needle 1 is provided with a sampling channel 103 and an air release channel 104, the driving member 2 is communicated with the sampling channel 103, and the sampling method includes:

the sampling needle 1 punctures the test tube, the air release passage 104 is communicated with the outside, so that the pressure in the test tube is balanced with the outside to be normal pressure;

when the pressure in the test tube is normal pressure, the driving member 2 drives the sampling channel 104 of the sampling needle 1 to suck the sample in the test tube.

In this embodiment, include sampling channel 103 and air bleed channel 104 through setting up sampling needle 1 for sampling needle 1 is after the puncture test tube, and the pressure in the test tube is the ordinary pressure through air bleed channel 104 and external balance, makes driving piece 2 can drive sampling needle 1 and inhale the appearance, and the sample gets into in the sampling channel 103, thereby realizes the purpose that the appearance was inhaled in the puncture once, has accelerated sample analyzer's sampling speed and measuring speed.

In one embodiment, the sampling assembly includes a constant pressure member and a first switching member 11, the constant pressure member is in communication with the outside or the internal pressure is constant pressure, and the air release passage 104 is in communication with the outside and includes: the air release passage 104 communicates with the constant pressure member, and the first switching member 11 communicates with or shuts off the air release passage 104 and the constant pressure member.

In one embodiment, referring to fig. 4, the constant pressure component is a swab 3, the swab 3 has an atmosphere end, the atmosphere end is connected to the outside, the air release channel 104 is connected to the atmosphere end, and the sampling method includes:

the air release passage 104 is communicated with air at the atmosphere end to balance the pressure in the test tube with the external pressure and achieve the normal pressure.

In one embodiment, after the sampling needle 1 finishes the sampling and withdraws from the test tube, the sampling method further comprises the step of cleaning the sampling needle, and the first switching member 11 is communicated to introduce liquid into the air release passage 104 for cleaning.

In one embodiment, a first three-way joint 12 is arranged between the first switching piece 11 and the atmosphere end, the first three-way joint 12 is further communicated with a first liquid tank 15, and a second switching piece 14 is arranged between the first three-way joint 12 and the first liquid tank 15, and the sampling method includes:

the second switching member 14 communicates the first liquid tank 15 with the first three-way member 12, and the liquid in the first liquid tank 15 flows into the atmosphere end of the swab 3 through the first three-way member 12 to clean the outer wall of the sampling needle 1.

In one embodiment, the method further comprises:

the first switching member 11 connects the air bleeding passage 104 with the first three-way member 12, and the liquid in the first liquid tank 15 flows into the air bleeding passage 104 through the first three-way member 12 to clean the inner wall of the air bleeding passage 104.

In one embodiment, the sampling method further comprises:

when the sampling needle 1 finishes the sample division, the driving piece 2 is led into the liquid to clean the sampling channel 103 of the sampling needle 1.

In one embodiment, a second three-way joint 13 is disposed between the first three-way joint 12 and the second switch 14, the second three-way joint 13 is connected to a positive pressure source 18, a third switch 17 is disposed between the second three-way joint 13 and the positive pressure source 18, and after the deflation channel 104 of the sampling needle 1 is cleaned, the sampling method further includes a step of drying the deflation channel 104, including:

the third switching member 17 communicates the positive pressure source 18 with the second three-way joint 13, the first switching member 11 communicates the air release passage 104 with the first three-way joint 12, and the gas of the positive pressure source 18 enters the air release passage 104 through the third switching member 17, the second three-way joint 13, the first three-way joint 12 and the first switching member 11.

In one embodiment, a fourth switching member 16 is disposed between the second three-way joint 13 and the third switching member 17, and the sampling method further includes:

when the step of cleaning the sampling needle 1 is performed, the fourth switching member 16 cuts off the second three-way joint 13 and the third switching member 17, and prevents the liquid of the first liquid tank 15 from flowing into the third switching member 17;

when the step of blow-drying the air bleeding passage 104 is performed, the fourth switching piece 16 communicates the second three-way joint 13 and the third switching piece 17.

In one embodiment, referring to fig. 5 in combination with fig. 1, the diameter of the pipe between the second three-way joint 13 and the third switching member 17 is smaller than the diameter of the other pipe through which the liquid of the first liquid tank 15 flows, and the sampling method further includes:

when the step of cleaning the sampling needle 1 is performed, the liquid of the first liquid tank 15 flows into the atmosphere end of the first switch piece 11 and/or the swab 3 at the second three-way joint 13;

when the step of blow-drying the air release passage 104 is performed, the flow rate of the gas of the positive pressure source 18 in the pipe between the second three-way joint 13 and the third switch 17 is faster to accelerate the blow-drying speed.

In one embodiment, referring to fig. 6 in combination with fig. 1, a sixth switch 32 is disposed between the first three-way joint 12 and the atmosphere end of the swab 3, and the sampling method further includes:

when the sampling needle 1 exits the test tube, the sixth switching member 32 cuts off the first three-way joint 12 from the atmosphere end, so that the liquid of the first liquid tank 15 flows into the air release passage 104 and does not flow into the atmosphere end, thereby saving the liquid for cleaning.

In one embodiment, referring to fig. 7 in combination with fig. 1, the normal pressure component is a normal pressure source 19, and the pressure in the normal pressure source 19 is normal pressure, the sampling method includes:

after the sampling needle 1 penetrates into the test tube, the first switching piece 11 is communicated with the air release channel 104 and the normal pressure source 19, and the air release channel 104 achieves pressure balance through air communication with the normal pressure source 19.

In one embodiment, the sampling assembly further comprises a positive voltage source 18 and a third switch 17, and the sampling method further comprises:

when the sampling needle 1 has pierced into the test tube, the third switch 17 connects the normal pressure source 19 with the first switch 11;

when the sampling needle 1 is cleaned, the third switch 17 communicates the positive pressure source 18 with the first switch 11.

In one embodiment, a second three-way joint 13 is arranged between the air bleeding channel 104 and the first switching member 11, the second three-way joint 13 is communicated with the second switching member 14 and the first liquid tank 15 in sequence, and the sampling method further comprises:

when cleaning of the sampling needle 1 is performed, the first switch 11 cuts off the third switch 17 and the second three-way joint 13, and the liquid of the first liquid tank 15 flows into the air bleeding passage 104 through the second switch 14 and the second three-way joint 13.

In an embodiment, referring to fig. 8 in combination with fig. 1, an eighth switch 4 is disposed between the sampling channel 103 and the driving member 2, and the sampling method further includes:

when the sampling needle 1 pierces the test tube and the pressure in the test tube is not balanced, the eighth switch 4 disconnects the sampling passage 103 from the driving member 2.

While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.