阅读说明：本技术 样本分析仪及其采样方法 (Sample analyzer and sampling method thereof ) 是由 冯祥 刘隐明 于 2020-06-01 设计创作，主要内容包括：一种样本分析仪及其采样方法,该样本分析仪包括驱动组件、采样组件、反应组件、检测组件、废液处理组件以及控制器,其中,采样组件包括采样针、第一管路和压力调节组件,所述采样针设有采样通道,所述第一管路与所述采样通道连通,所述压力调节组件设于所述第一管路上和/或连通所述第一管路,所述压力调节组件用于平衡或抵消所述采样通道内的压力。通过压力调节组件来平衡或抵消采样针的采样通道的压力,以消除试管内的压力对采样准确性所产生不利影响,利用所述样本分析仪进行采样时,只需要令所述采样针进行一次穿刺即可完成采样,不再需要进行穿刺预处理和穿刺预处理后清洗采样针的工序,缩短了采样时间,提高了采样速度。(A sample analyzer comprises a driving assembly, a sampling assembly, a reaction assembly, a detection assembly, a waste liquid treatment assembly and a controller, wherein the sampling assembly comprises a sampling needle, a first pipeline and a pressure adjusting assembly, the sampling needle is provided with a sampling channel, the first pipeline is communicated with the sampling channel, the pressure adjusting assembly is arranged on the first pipeline and/or communicated with the first pipeline, and the pressure adjusting assembly is used for balancing or offsetting the pressure in the sampling channel. The pressure of the sampling channel of the sampling needle is balanced or offset through the pressure adjusting assembly to eliminate adverse effects of the pressure in the test tube on sampling accuracy.)

1. A sample analyzer is characterized by comprising a driving component, a sampling component, a reaction component, a detection component, a waste liquid treatment component and a controller; the drive assembly is used for driving a flow path in the sample analyzer; the sampling assembly is used for collecting and distributing biological samples; the reaction component is used for processing the biological sample to form a liquid to be detected; the detection assembly is used for detecting the liquid to be detected to form detection information; the waste liquid treatment component is used for collecting and discharging waste liquid in the sample analyzer; the controller is used for controlling the work flow of the sample analyzer and processing the detection information to form an analysis result;

wherein, the sampling subassembly includes sampling needle, first pipeline and pressure adjustment subassembly, the sampling needle is equipped with the sampling passageway, first pipeline with the sampling passageway intercommunication, the pressure adjustment subassembly is located on the first pipeline and/or the intercommunication first pipeline, the pressure adjustment subassembly is used for balancing or offsetting pressure in the sampling passageway.

2. The sample analyzer of claim 1, wherein the pressure adjustment assembly includes a first switch member and a drive member, an end of the first conduit remote from the sampling needle communicates with the drive member, the first switch member is disposed on the first conduit, and the first switch member is configured to communicate with or disconnect the drive member from the sampling needle.

3. The sample analyzer as claimed in claim 2, wherein the sampling assembly further comprises a first detecting member disposed on the first pipeline, the first detecting member is used for detecting whether the liquid column height of the sample sucked by the sampling needle reaches a preset height, so as to correspondingly determine whether to close the first switching member to cut off the sampling needle and the driving member.

4. The sample analyzer of claim 3, wherein the first switch member is located at a distance from the sampling needle that is less than the distance from the driving member, and the first detection member is disposed on a side of the first switch member remote from the sampling needle or between the first switch member and the driving member.

5. The sample analyzer of claim 2, wherein a second sensing member is disposed on the first conduit between the first switch member and the driving member, the second sensing member being configured to sense a pressure of the first conduit between the first switch member and the driving member in real time.

6. The sample analyzer of claim 2 wherein the sampling assembly further comprises a reservoir, the reservoir being connected to the drive member, the reservoir being in communication with the drive member when pressure is to be released from the first line between the first switch member and the drive member.

7. The sample analyzer of claim 6 wherein the sampling assembly further comprises a constant pressure source coupled to the reservoir and configured to provide a constant pressure to the reservoir.

8. The sample analyzer of claim 6 wherein a second switch is provided between the drive member and the reservoir for communicating the drive member with the reservoir when pressure is to be released from the first conduit between the first switch and the drive member.

9. The sample analyzer of claim 8 wherein the sampling assembly further comprises a positive pressure source coupled to the reservoir for pressurizing the reservoir; when the sampling needle divides the appearance to accomplish the back, the malleation source is to the liquid reserve tank pressure boost, the second switches the piece intercommunication the driving piece with the liquid reserve tank, first switching piece intercommunication the sampling needle with the driving piece makes the liquid inflow of liquid reserve tank the driving piece, and the warp first pipeline flows in the sampling passageway of sampling needle, it is right the sampling passageway washs.

10. The sample analyzer of claim 9, wherein a third switch is provided between the positive pressure source, the constant pressure source and the reservoir, the third switch is used to connect or disconnect the positive pressure source or the constant pressure source and the reservoir, the third switch connects the positive pressure source and the reservoir when the sampling channel needs to be cleaned, and the third switch connects the constant pressure source and the reservoir when the pressure needs to be released from the first pipeline between the first switch and the driving member.

11. The sample analyzer of claim 6, wherein the sampling assembly further comprises a reagent barrel and a negative pressure source, both the reagent barrel and the negative pressure source being connected to the reservoir, the negative pressure source being configured to depressurize the reservoir; when the liquid storage tank needs to be filled, the liquid storage tank is communicated with the negative pressure source and the reagent barrel, and liquid in the reagent barrel flows into the liquid storage tank under the action of pressure difference.

12. The sample analyzer of claim 8, wherein a first tee joint is provided on a first conduit between the first switch and the drive member, the reservoir is connected to the first tee joint, and the second switch is provided between the first tee joint and the reservoir.

13. The sample analyzer of claim 8 wherein the sampling assembly further comprises a swab, the swab including an atmospheric end, a second three-way joint is provided on the first conduit between the first switch and the driving member, and a sixth switch is provided between the atmospheric end of the swab and the second three-way joint for connecting or disconnecting the first conduit and the atmospheric end of the swab.

14. The sample analyzer of claim 13, wherein a third tee is disposed between the sixth switch and the atmospheric end of the swab, a fourth tee is disposed between the second switch and the reservoir, and a seventh switch is disposed between the third tee and the fourth tee for communicating the third tee and the fourth tee.

15. The sample analyzer of claim 2 wherein the first conduit between the first switch member and the sampling needle has a hardness greater than the hardness of the first conduit between the first switch member and the drive member.

16. The sample analyzer of claim 1 further comprising a driving member connected to an end of the first conduit remote from the sampling needle, the first conduit having a deformability, wherein the pressure regulating assembly is the driving member, wherein the driving member establishes an isolation gas column in the sampling needle before sampling, and wherein the amount of sample drawn by the sampling needle is controlled to counteract the effects of in vitro pressure and deflection of the first conduit on the sample in the sampling needle.

17. The sample analyzer of claim 16 wherein the volume of the isolated gas column is not less than 12 uL.

18. The sample analyzer of claim 1 further comprising a drive member coupled to an end of the first conduit distal from the sampling needle, wherein the first conduit is a rigid conduit and the pressure regulating assembly is the drive member, wherein the drive member establishes a column of barrier gas within the sampling needle prior to sampling and controls a sample volume of the sampling needle to counteract an offset effect of in vitro pressure on the sample within the sampling needle.

19. The sample analyzer of claim 18 wherein the first conduit is a steel tube and the first conduit has a length of 10mm to 50 mm.

20. A sampling method of a sample analyzer, wherein a sampling assembly of the sample analyzer comprises a sampling needle, a first pipeline and a pressure regulating assembly, the sampling needle is provided with a sampling channel, the first pipeline is communicated with the sampling channel, the pressure regulating assembly is arranged on the first pipeline and/or communicated with the first pipeline, and the sampling method comprises the following steps:

the sampling needle penetrates into the test tube to suck a sample, so that the sample enters the sampling channel;

the pressure regulating assembly balances or counteracts the pressure within the sampling channel.

21. The sampling method of claim 20, wherein the pressure regulating assembly further comprises a first switch and a driver, an end of the first tubing distal to the sampling needle communicating with the driver, the first switch disposed on the first tubing, the sampling method comprising:

when the sampling needle punctures and enters the test tube, the driving piece drives the sampling needle to suck samples, and after the samples enter the sampling channel, the first switching piece cuts off the sampling channel and the driving piece.

22. The sampling method of claim 21, wherein the sampling method further comprises:

when the first switching piece cuts off the sampling channel and the driving piece, the driving piece drives, so that the pressure of the first pipeline between the first switching piece and the driving piece is balanced with the outside.

23. The sampling method of claim 22, wherein a second detector is disposed on the first conduit between the first switching member and the driving member, the sampling method comprising:

the second detection piece detects the pressure of the first pipeline between the first switching piece and the driving piece in real time, and the driving piece adjusts the pressure of the first pipeline between the first switching piece and the driving piece according to the pressure detected by the second detection piece until the pressure of the first pipeline is balanced with the outside.

24. The sampling method of claim 21, wherein the sampling assembly further comprises a first detection member disposed on the first line, the sampling method further comprising:

when the first detection piece detects that the liquid column height of the sample in the sampling channel reaches a preset height, the first switching piece cuts off the sampling needle and the driving piece.

25. The sampling method of claim 21, wherein the sampling assembly further comprises a reservoir coupled to the drive member, the sampling method comprising:

the liquid storage tank is communicated with the driving piece so as to release the pressure of the first pipeline between the first switching piece and the driving piece.

26. The sampling method of claim 25, wherein the sampling assembly further comprises a constant pressure source connected to the reservoir for providing a constant pressure to the reservoir, wherein the reservoir is configured to communicate with the actuator when pressure needs to be relieved from the first line between the first switch member and the actuator, and wherein the constant pressure source communicates with the reservoir.

27. The sampling method of claim 25, wherein a second switch is provided between said drive member and said reservoir, and wherein said sampling method comprises, when pressure is to be released from said first line between said first switch and said drive member:

the second switching piece is communicated with the driving piece and the liquid storage tank.

28. The sampling method of claim 27, wherein said sampling assembly further comprises a positive pressure source connected to said reservoir for pressurizing said reservoir, said sampling method comprising:

when the sampling needle divides the appearance to accomplish the back, the malleation source is to the liquid reserve tank pressure boost, the second switches the piece intercommunication the driving piece with the liquid reserve tank, first switching piece intercommunication the sampling needle with the driving piece makes the liquid inflow of liquid reserve tank the driving piece, and the warp first pipeline flows in the sampling passageway of sampling needle, it is right the sampling passageway washs.

29. The sampling method of claim 28, wherein a third switch is provided between the positive pressure source, the constant pressure source, and the reservoir, the sampling method comprising:

when the sampling channel needs to be cleaned, the third switching piece is communicated with the positive pressure source and the liquid storage tank; and/or

When pressure needs to be released from the first pipeline between the first switching piece and the driving piece, the third switching piece is communicated with the constant pressure source and the liquid storage tank.

30. The sampling method of claim 28, wherein the sampling assembly further comprises a reagent barrel and a negative pressure source, both connected to the reservoir, the negative pressure source for depressurizing the reservoir, the sampling method further comprising:

when the liquid storage tank needs to be filled, the liquid storage tank is communicated with the negative pressure source and the reagent barrel, and liquid in the reagent barrel flows into the liquid storage tank under the action of pressure difference.

31. The sampling method of claim 27, wherein the sampling assembly further comprises a swab, the swab comprising an atmospheric end, wherein a second three-way joint is provided in the first conduit between the first switching element and the driving element, wherein a sixth switching element is provided between the atmospheric end of the swab and the second three-way joint, and wherein the sampling method further comprises:

when pressure needs to be released from the first pipeline between the first switching piece and the driving piece, the sixth switching piece is communicated with the second three-way joint and the atmosphere end of the swab.

32. The sampling method of claim 31, wherein a third three-way joint is further provided between the sixth switch and the atmosphere end of the swab, a fourth three-way joint is further provided between the second switch and the reservoir, and a seventh switch is further provided between the third three-way joint and the fourth three-way joint, the sampling method further comprising:

when the pressure of the first pipeline between the first switching piece and the driving piece is released, the sixth switching piece cuts off the second three-way joint and the third three-way joint, the second switching piece cuts off the fourth three-way joint and the driving piece, the seventh switching piece is communicated with the third three-way joint and the fourth three-way joint, and the liquid in the liquid storage tank is introduced into the atmosphere end of the swab so as to clean the outer wall of the sampling needle.

33. The sampling method of claim 20, wherein the sampling assembly further comprises a driving member coupled to an end of the first tubing remote from the sampling needle, the first tubing having a deformability, the pressure adjustment assembly being the driving member, the sampling method comprising:

the driving piece establishes an isolation gas column in the sampling needle before sampling, and controls the sample suction amount of the sampling needle to offset the offset influence of the pressure in the test tube and the deformation of the first pipeline on the sample in the sampling needle.

34. The sampling method of claim 33, wherein the volume of the column of barrier gas is not less than 12 uL.

35. The sampling method of claim 20, wherein the sampling assembly further comprises a drive member coupled to an end of the first tubing distal to the sampling needle, the first tubing is rigid tubing, the pressure regulating assembly is the drive member, the sampling method comprising:

the driving part establishes an isolation gas column before sampling, and controls the sample suction amount of the sampling needle to offset the offset influence of the pressure in the test tube on the sample in the sampling needle.

36. The sampling method of claim 35, wherein the first tubing is steel tubing and the length of the first tubing is 10mm to 50 mm.

Technical Field

The invention relates to the technical field of medical instruments, in particular to a sample analyzer and a sampling method thereof.

Background

The sample analyzer needs to extract the biological sample from the closed test tube for analysis, and because the pressure generally exists in the closed test tube, the pressure can produce adverse effect to the sampling accuracy, so how to solve the problem that accurate sampling from the closed test tube is generally faced in the industry.

The current scheme usually adopts two punctures, the first puncture of sampling needle is puncture preliminary treatment to release the pressure in the test tube, then wash the sampling needle, and then the sampling needle carries out the second puncture in order to absorb the biological sample. Because the sampling needle needs to puncture twice and needs to be cleaned in the middle of the two punctures, the sampling speed of the sample analyzer is directly limited, and the measurement speed of the sample analyzer is slow.

Disclosure of Invention

In view of the above, an object of the present invention is to provide a sample analyzer and a sampling method thereof, which can complete sampling by only one-time puncturing with the sampling needle without performing two-time puncturing, thereby shortening sampling time and increasing sampling speed.

In order to realize the purpose of the invention, the invention provides the following technical scheme:

in a first aspect, the present invention provides a sample analyzer, comprising a driving component, a sampling component, a reaction component, a detection component, a waste liquid treatment component and a controller; the drive assembly is used for driving a flow path in the sample analyzer; the sampling assembly is used for collecting and distributing biological samples; the reaction component is used for processing the biological sample to form a liquid to be detected; the detection assembly is used for detecting the liquid to be detected to form detection information; the waste liquid treatment component is used for collecting and discharging waste liquid in the sample analyzer; the controller is used for controlling the work flow of the sample analyzer and processing the detection information to form an analysis result;

wherein, the sampling subassembly includes sampling needle, first pipeline and pressure adjustment subassembly, the sampling needle is equipped with the sampling passageway, first pipeline with the sampling passageway intercommunication, the pressure adjustment subassembly is located on the first pipeline and/or the intercommunication first pipeline, the pressure adjustment subassembly is used for balancing or offsetting pressure in the sampling passageway.

In a second aspect, an embodiment of the present invention provides a sampling method for a sample analyzer, where a sampling assembly of the sample analyzer includes a sampling needle, a first pipeline, and a pressure regulating assembly, the sampling needle is provided with a sampling channel, the first pipeline is communicated with the sampling channel, the pressure regulating assembly is provided on the first pipeline and/or is communicated with the first pipeline, and the sampling method includes:

the sampling needle penetrates into the test tube to suck a sample, so that the sample enters the sampling channel;

the pressure regulating assembly balances or counteracts the pressure within the sampling channel.

According to the sample analyzer provided by the invention, the pressure of the sampling channel of the sampling needle is balanced or offset through the pressure adjusting assembly so as to eliminate the adverse effect of the pressure in the test tube on the sampling accuracy.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic diagram of a sampling assembly of a sample analyzer according to an embodiment;

FIG. 2 is a schematic diagram of a sampling assembly of the sample analyzer of an embodiment;

FIG. 3 is a schematic diagram of a sampling assembly of the sample analyzer of an embodiment;

FIG. 4 is a schematic diagram of a sampling assembly of the sample analyzer of an embodiment;

FIG. 5 is a schematic diagram of a sampling assembly of the sample analyzer of an embodiment;

FIG. 6 is a schematic illustration of the effect of in vitro pressure on the sample in the sampling needle of one embodiment;

FIG. 7 is a schematic diagram of a sampling assembly of the sample analyzer of an embodiment;

FIG. 8 is a schematic view of the sample suction volume of a sample analyzer of an embodiment;

FIG. 9 is a schematic diagram of a sampling assembly of the sample analyzer of an embodiment;

FIG. 10 is a schematic view of the sample amount of a sample analyzer according to an embodiment.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

The embodiment of the invention provides a sample analyzer. The sample analyzer may be used to perform biological sample analysis, and the biological sample may be blood, urine, or the like. The sample analyzer includes a sampling assembly for collecting and dispensing a biological sample. The sample analyzer also includes a drive assembly, a reaction assembly, a detection assembly, a waste liquid treatment assembly, and a controller. The drive assembly is used to drive various flow paths (including gas and liquid paths) in the sample analyzer. The reaction assembly is used for processing the biological sample to form a liquid to be detected. The detection component is used for detecting the liquid to be detected to form detection information. The waste liquid treatment assembly is used for collecting and discharging waste liquid in the sample analyzer. The controller is used for controlling the work flow of the sample analyzer and processing the detection information to form an analysis result.

Referring to fig. 1, 7 and 9, the sampling assembly includes a sampling needle 1, a first pipeline 15 and a pressure regulating assembly, the sampling needle 1 is provided with a sampling channel (not shown in the figure), the first pipeline 15 is communicated with the sampling channel, the pressure regulating assembly is provided on the first pipeline 15 and/or communicated with the first pipeline 15, and the pressure regulating assembly is used for balancing or offsetting the pressure in the sampling channel.

In this embodiment, come the pressure of balance or offset sampling needle 1's sampling passageway through the pressure adjustment subassembly to eliminate the produced adverse effect of pressure in the test tube to the sampling accuracy, when utilizing the sampling subassembly to sample, only need make sampling needle 1 carry out a puncture and can accomplish the sampling, no longer need carry out puncture preliminary treatment and puncture preliminary treatment after wash the process of sampling needle, shortened sampling time, improved sampling speed. Because the sampling flow is a key path for measuring the sample analyzer, the sampling assembly is used for sampling, so that the measuring time of the sample analyzer is shortened, and the measuring speed of the sample analyzer is improved. Meanwhile, the sampling assembly is utilized to sample, only one puncture is needed, the abrasion to the sampling needle 1 can be reduced, and the service life of the sampling needle 1 is prolonged.

In one embodiment, referring to fig. 1, the pressure regulating assembly includes a first switching member 11, a first pipeline 15 and a driving member 2, wherein an end of the first pipeline 15 away from the sampling needle 1 is communicated with the driving member 2, the first switching member 11 is disposed on the first pipeline 15, and the first switching member 11 is used for communicating or disconnecting the driving member 2 and the sampling needle 1.

In this embodiment, in sampling needle 1 puncture test tube and stretched into biological sample, after 2 drive sampling needles of driving piece 1 accomplished the appearance of inhaling, first switching piece 11 cuts off sampling needle 1 and driving piece 2 being connected to reduce sampling needle 1 and withdraw from the influence of in-process test tube internal pressure, later withdraw from the test tube with sampling needle 1 again. Because the pressure of the test tube can pressurize or decompress the biological sample in the test tube when the sampling needle 1 sucks the sample, and the pressure is transmitted to the first pipeline 15 and the driving part 2 through the sampling needle 1, and the first switching member 11 cuts off the connection between the sampling needle 1 and the driving member 2, so that the pressure in the first pipeline 15 from the first switching member 11 to the driving member 2 is related to the pressure in the test tube, the pressure of the first pipeline 15 between the first switching piece 11 and the sampling needle 1 is the same as the atmospheric pressure after the sampling needle 1 exits the test tube, the pressure of the first pipeline 15 between the first switching piece 11 and the driving piece 2 can be the same as the atmospheric pressure by adjusting the driving piece 2, then the first switching piece 11 is communicated with the sampling needle 1 and the driving piece 2, make first pipeline 15 the pressure before first switching piece 11 back the same, eliminated the influence of test tube pressure to the sampling, only need once the puncture can. The drive member 2 may be a syringe. The first switch 11 may be a two-position three-way solenoid valve.

Preferably, the first pipeline 15 is a rubber hose, especially a hard rubber hose, and the pressure borne by the first pipeline 15 causes the first pipeline 15 to deform during the sample suction process of the sampling needle 1. After the sampling needle 1 exits the test tube, the first pipeline 15 is restored to the original state in the process of balancing the pressure by the driving part 2. The deformation of the first pipeline 15 is used for offsetting the pressure action of the pressure in the test tube on the sampling assembly, so that the puncture for the deflation of the test tube can be reduced. In addition, after the first switching piece 11 cuts off the connection between the sampling needle 1 and the driving piece 2, the first pipeline 15 between the sampling needle 1 and the first switching piece 11 can be influenced by the pressure in the test tube before and after the sampling needle 1 exits from the test tube, so a hard rubber tube is preferably used for the first pipeline 15 between the first switching piece 11 and the sampling needle 1, the deformation of the hard rubber tube is small before and after the sampling needle 1 exits from the test tube, and the influence on the biological sample sucked in the sampling needle 1 is small. Preferably, the first switching member 11 is arranged close to the distal outlet of the sampling needle 1, i.e. the distance from the first switching member 11 to the sampling needle 1 is smaller than the distance to the driving member 2, so as to shorten the length of the first pipeline 15 between the first switching member 11 and the sampling needle 1 as much as possible and reduce the influence of the deformation of the first pipeline 15.

In one embodiment, a first detecting member 12 is disposed on a side of the first switching member 11 away from the sampling needle 1, and the first detecting member 12 is used for detecting whether a liquid column height of a sample sucked by the sampling needle 1 reaches a preset height. When the first detecting member 12 detects the liquid column of the sample, it indicates that the sample drawn by the sampling needle 1 is sufficient, and the sample drawing can be stopped. The first sensing member 12 may be an opto-coupler sensor.

In this embodiment, when the first detecting member 12 detects the liquid column of the sample, the first switching member 11 cuts off the sampling needle 1 and the driving member 2, and then withdraws the sampling needle 1 from the test tube.

In another embodiment, referring to fig. 2, the first detecting member 12 is disposed between the sampling needle 1 and the first switching member 11. Compared with the solution that the first detection member 12 is disposed on the side of the first switching member 11 far away from the sampling needle 1, the length of the first switching member 11 to the first pipeline 15 at the end of the sampling needle 1 in the present embodiment is slightly longer, and the influence of the deformation of the first pipeline 15 on the sample sucked by the sampling needle 1 is slightly larger.

In one embodiment, with continued reference to fig. 1, the driving member 2 is connected to a reservoir 3, and the reservoir 3 is connected to a constant pressure source 35. The normal pressure source 35 provides normal pressure for the liquid storage tank 3, when the first switching piece 11 cuts off the sampling needle 1 and the driving piece 2 and pressure needs to be released to the first pipeline 15 between the first switching piece 1 and the driving piece 2, the liquid storage tank 3 is communicated with the driving piece 2, the normal pressure source 35 is communicated with the liquid storage tank 3, so that the first pipeline 15 between the first switching piece 11 and the driving piece 2 is communicated with the liquid storage tank 2, and the first pipeline 15 is released to the normal pressure. When the sampling is performed at atmospheric pressure, normal pressure refers to atmospheric pressure; when sampling is performed in a closed space, normal pressure refers to the air pressure of the closed space in which sampling is performed. The reservoir 3 is used for storing a liquid, such as a diluent.

In this embodiment, a second switching member 21 is disposed between the driving member 2 and the liquid storage tank 3, and the second switching member 21 is used for connecting or disconnecting the driving member 2 and the liquid storage tank 3.

When the sampling needle 1 does not puncture the test tube, or the sampling needle 1 punctures the test tube and the sample suction port of the sampling needle 1 is immersed in the biological sample, the first switch 11 cuts off the sampling needle 1 and the driving member 2, and the second switch 21 cuts off the driving member 2 and the liquid storage tank 3. In the process that the driving piece 2 drives the sampling needle 1 to suck samples, the first switching piece 11 is communicated with the sampling needle 1 and the driving piece 2, and the second switching piece 21 cuts off the driving piece 2 and the liquid storage tank 3. After the sample suction is completed, the first switching member 11 cuts off the sampling needle 1 and the driving member 2. When the first switching member 11 cuts off the sampling needle 1 and the driving member 2, the sampling needle 1 starts to withdraw from the test tube, and the second switching member 21 communicates the driving member 2 with the reservoir 3 to make the liquid in the reservoir 3 flow and release the pressure of the first pipeline 15. The second switching member 21 may be a two-position three-way solenoid valve.

In this embodiment, after the pressure in the first pipeline 15 is released, the first switching member 11 communicates with the sampling needle 1 and the driving member 2, while the second switching member 21 continues to communicate with the driving member 2 and the liquid storage tank 3, and the driving member 2 drives the sampling needle 1 to sample.

Specifically, after the pressure in the first tube 15 is released, the pressure in the first tube 15 before and after the first switch 11 is set to the normal pressure, and the sample separation is performed under the normal pressure, so that the sample liquid column in the sampling needle 1 is kept stable and does not flow out or flow in the direction of the first tube 15 under the pressure in the first tube 15. When the sample is divided, taking the driving piece 2 as an injector as an example, the injector pushes a small section forwards to discharge a little sample at the front end of the sampling needle 1, then the injector is pushed forwards continuously, and the sampling needle 1 drops the sample into the reaction pool. Preferably, the sample can be dropped into a plurality of reaction wells at one time.

In one embodiment, the sampling assembly further comprises a positive pressure source 34, the positive pressure source 34 being connected to the reservoir 3 for pressurizing the reservoir 3. After sampling needle 1 divides the appearance to accomplish, malleation source 34 is to liquid storage tank 3 pressure boost, and second switching piece 21 intercommunication driving piece 2 and liquid storage tank 3, first switching piece 11 intercommunication sampling needle 1 and driving piece 2 for liquid in the liquid storage tank 3 flows into driving piece 2, in the sampling passageway of sampling needle 1 is flowed into through first pipeline 15, washs the sampling passageway.

Preferably, a third switch 32 is further provided between the positive pressure source 34, the normal pressure source 35, and the reservoir 3, and the third switch 32 is used for connection or disconnection.

Specifically, the third switch 32 is used to connect or disconnect the positive pressure source 34 and the reservoir 3, and is also used to connect or disconnect the constant pressure source 35 and the reservoir 3. When the sampling channel of the sampling needle 1 is cleaned, the third switch 32 connects the positive pressure source 34 to the reservoir 3, and disconnects the constant pressure source 35 from the reservoir 3. When the sampling needle 1 samples, withdraws the test tube and sorts the sample, the third switch 32 disconnects the positive pressure source 34 from the reservoir 3 and connects the normal pressure source 35 to the reservoir 3. The third switch 32 may be a two-position three-way solenoid valve.

In one embodiment, the sampling assembly further comprises a reagent barrel 4 and a negative pressure source 33, wherein the reagent barrel 4 and the negative pressure source 33 are both connected with the liquid storage tank 3, the reagent barrel 4 is used for storing liquid, and the negative pressure source 33 is used for reducing the pressure of the liquid storage tank 3, so that the negative pressure is generated in the liquid storage tank 3. When the liquid storage tank 3 is communicated with the negative pressure source 33 and the reagent barrel 4, the liquid in the reagent barrel 4 flows into the liquid storage tank 3 under the action of pressure difference, and the liquid storage tank 3 is filled.

Preferably, the sampling assembly further comprises a fourth switching member 31 and a fifth switching member 41, the fourth switching member 31 is arranged among the negative pressure source 33, the third switching member 32 and the liquid storage tank 3, the fifth switching member 41 is arranged among the reagent barrel 4 and the liquid storage tank 3, and both the fourth switching member 31 and the fifth switching member 41 are used for connection or disconnection.

Specifically, the fourth switching member 31 is used to connect or disconnect the third switching member 32 and the reservoir 3, and also used to connect or disconnect the negative pressure source 33 and the reservoir 3. The fifth switching member 41 is used to connect or disconnect the reagent cartridge 4 and the reservoir 3. The fourth switching member 31 and the fifth switching member 41 may be two-position three-way solenoid valves.

In one embodiment, the sampling assembly further comprises a swab 5, the swab 5 being adapted to clean the outer wall of the sampling needle 1 and to recover waste liquid after cleaning.

Specifically, after the sampling needle 1 finishes sampling and is withdrawn from the test tube, the swab is used to wash the outer wall of the sampling needle 1, and waste liquid is recovered.

Furthermore, after the sampling needle 1 finishes sample separation, the outer wall of the sampling needle 1 can be cleaned again, and a sampling channel in the sampling needle 1 is cleaned at the same time. The cleaned sampling needle 1 is used for the next sampling. Wherein, after the completion of the sampling passageway of washing sampling needle 1, driving piece 2 resets, and second switching piece 21 cuts off driving piece 2 and liquid storage tank 3's being connected to use driving piece 2 to establish the isolation gas column for sampling needle 1. Taking the driving member 2 as an injector as an example, the injector is first pushed forward by a small section and then retracted and reset, so that an isolated gas column can be established in the sampling needle 1.

In one embodiment, referring to fig. 3, a second detecting element 13 is disposed on the first pipeline 15 between the first switching element 11 and the driving element 2, and the second detecting element 13 is used for detecting the pressure of the first pipeline 15 between the first switching element 11 and the driving element 2 in real time.

In this embodiment, when the sampling needle 1 is sucking a sample, the first switching member 11 communicates with the sampling needle 1 and the driving member 2, the second switching member 21 communicates with the driving member 2 and the liquid storage tank 3, the liquid storage tank 3 may not be provided with the normal pressure source 35 (refer to fig. 1) connected thereto, the driving member 2 drives the sampling needle 1 to suck a sample, and the second detecting member 13 detects the pressure of the first pipeline 15. After sampling needle 1 withdraws from the test tube, use driving piece 2 to adjust the pressure of first pipeline 15 between first switching piece 11 and the driving piece 2 to make the pressure numerical value that second detection piece 13 detected be the pressure numerical value when the ordinary pressure, first switching piece 11 intercommunication sampling needle 1 and driving piece 2, the pressure balance of first pipeline 15 around first switching piece 11 this moment makes the liquid column of the sample in the sampling needle 1 keep stable, then can divide kind.

In particular, taking the driving member 2 as an example of an injector, the injector can also be pushed forward or pulled backward after the sampling needle 1 is withdrawn from the test tube. When the pressure of the first pipeline 15 detected by the second detection piece 13 is normal pressure, the injector does not act; when the pressure of the first pipeline 15 detected by the second detection piece 13 is positive pressure, the injector is pulled backwards until the pressure of the first pipeline 15 detected by the second detection piece 13 is normal pressure, and the action of the injector is stopped; when the pressure of the first pipeline 15 detected by the second detection piece 13 is negative pressure, the injector is pushed forward until the pressure of the first pipeline 15 detected by the second detection piece 13 is normal pressure, and the action of the injector is stopped. The second detector 13 is a pressure sensor, preferably a hydraulic pressure sensor.

In this embodiment, the second detecting element 13 is arranged, the setting of the normal pressure source is reduced, and the pressure of the first pipeline 15 is adjusted through the driving element 2, so that the structure is simpler, the cost of the whole sample analyzer is reduced due to the fact that the second detecting element 13 for detecting the pressure is added is low, and the normal pressure source is reduced.

In one embodiment, referring to fig. 4, a first three-way joint 14 is disposed on the first pipeline 15 between the first switching member 11 and the driving member 2, the liquid storage tank 3 is connected to the first three-way joint 14, and the second switching member 21 is disposed between the first three-way joint 14 and the liquid storage tank 3.

In this embodiment, three joints of the first three-way joint 13 are connected to the first pipeline 15 on the first switching member 11 side, the first pipeline 15 on the driving member 2 side, and the pipeline on the second switching member 21 side, respectively. When the pressure of the first pipeline 15 between the first switching piece 11 and the driving piece 2 needs to be released, the second switching piece 21 is communicated with the liquid storage tank 3 and the first three-way joint 14, and at the moment, the liquid storage tank 3 is connected with a normal pressure source 35, so that the pressure balance of the first pipeline 15 in front of and behind the first switching piece 11 is constant pressure.

In one embodiment, referring to fig. 5, the swab 5 includes an atmosphere end, a second three-way joint 16 is disposed on the first pipeline 15 between the first switch 11 and the driving member 2, a sixth switch 17 is disposed between the atmosphere end of the swab 5 and the second three-way joint 16, and the sixth switch 17 is used for connecting or disconnecting the atmosphere end of the swab 5 to the first pipeline 15.

In this embodiment, the atmosphere end of the swab 5 is used for introducing liquid when the outer wall of the sampling needle 1 is cleaned, and the atmosphere end of the swab 5 is communicated with the atmosphere when the outer wall of the sampling needle 1 is not cleaned by the swab 5. When it is necessary to release the pressure of the first tube 15, the sixth switch 17 connects the first tube 15 to the atmosphere end of the swab 5 so that the pressure of the first tube 15 is atmospheric pressure, thereby equalizing the pressures of the first tubes 15 before and after the first switch 11. The sixth switching member 17 may be a two-position three-way solenoid valve. This embodiment may be used without the constant pressure source 35 (refer to fig. 1) on the reservoir 3.

In one embodiment, a third three-way joint 52 is further disposed between the sixth switching member 17 and the atmosphere end of the swab 5, a fourth three-way joint 23 is further disposed between the second switching member 21 and the liquid storage tank 3, a seventh switching member 53 is further disposed between the third three-way joint 52 and the fourth three-way joint 23, and the seventh switching member 53 is used for communicating or cutting off the third three-way joint 52 and the fourth three-way joint 23.

In this embodiment, when the outer wall of the sampling needle 1 needs to be cleaned, the reservoir 3 communicates with the positive pressure source 34, the seventh switch 53 communicates with the third three-way joint 52 and the fourth three-way joint 23, and the sixth switch 17 cuts off the second three-way joint 16 and the third three-way joint 52, so that the liquid in the reservoir 3 flows into the atmosphere end of the swab 5, and the outer wall of the sampling needle 1 is cleaned. The seventh switching piece 53 may be a two-position three-way solenoid valve.

Preferably, the swab 5 is further connected with a waste liquid recovery member 51, and the waste liquid recovery member 51 is used for recovering and cleaning the liquid at the time of cleaning the outer wall of the sampling needle 1.

Referring to fig. 6, when the pressure regulating assembly is not provided, since the sample suction port 101 (see fig. 6 (a)) is connected to the atmosphere after the sampling needle 1 exits the test tube, the difference between the test tube pressure and the atmospheric pressure may cause the sample in the sampling needle 1 to generate different states. After the sampling needle 1 sucks a sample and exits the test tube, the effect of the pressure in the test tube on the sample in the sampling needle 1 is as follows: referring to fig. 6 (a), when the pressure in the test tube is normal pressure, the sample extends from the sample inlet 101 in the sampling needle 1 to the sampling channel of the sampling needle 1, and the volume of the formed isolated gas column is the same as the volume of the isolated gas column established before sampling. Referring to fig. 6 (b), when the pressure in the test tube is negative pressure, the sampling channel of the sampling needle 1 extends from the sample suction port 101 (see fig. 6 (a)) by a negative pressure offset amount, the sample is entirely offset in a direction away from the sample suction port 101, and the volume of the formed isolation air column is compressed, so that the isolation air column is reduced relative to the isolation air column established before sampling. Referring to fig. 6 (c), when the pressure in the test tube is positive, the sampling channel of the sampling needle 1 extends a section of positive pressure offset from the isolated gas column established before sampling to the direction of the sample suction port 101, and the whole sample is offset to the direction close to the sample suction port 101, so that the isolated gas column established before sampling is increased.

In one embodiment, referring to fig. 7, in view of the effect of the in-tube pressure on the sampling shown in fig. 6, and in general, when the sample is divided after the sampling, a portion of the sample is discarded, and it is only necessary to ensure that the sample for the dividing is enough. The event provides a sampling subassembly, including sampling needle 1, first pipeline 15 and driving piece 2, first pipeline 15 has certain deformability, the pressure adjustment subassembly of this embodiment is driving piece 2 and first pipeline 15, sampling needle 1 is equipped with the sampling passageway, first pipeline 15 and sampling passageway intercommunication, and establish the isolation gas column through driving piece 2 before the sampling, inhale the appearance volume through driving piece control and offset the skew influence of the sample of in vitro pressure and the deformation of first pipeline 15 to sampling needle 1 sampling passageway.

In this embodiment, a large isolation air column is first established before sampling, so that when the pressure in the test tube is negative pressure, the isolation air column is reduced but will not disappear after the sampling needle 1 is withdrawn from the test tube. Furthermore, the sample sucked by the sampling needle 1 is driven by the driving member 2 to be divided into three sections, namely a first section sample, a measurement sample and a last section sample, wherein the first section sample is a section of sample near the sample sucking port 101, the last section sample is a section of sample near the isolation gas column, and the measurement sample is a section of sample between the first section sample and the last section sample. The first and last samples are discarded and the measurement samples are used for the sample separation. Finally, the deformation of the first tube 15 is set so that the pressure in the test tube and the deformation of the first tube 15 are added to ensure that enough measurement sample still exists in the sampling needle 1.

Specifically, referring to fig. 7 and fig. 8 (a), (b) and (c), the driving member 2 drives the sampling needle 1 to suck the sample, so that the first segment of the sample volume at least covers a portion of the sample sucking amount, and the last segment of the sample volume at least covers a portion of the sample sucking amount, and the whole sample is measured in the sample sucking amount. When the sample is divided, the front driving piece 2 drives the sample in the sampling needle 1 to discard at least part of the sample, namely, the first section of the sample is discarded, then the sample is divided, and finally the last section of the sample is discarded.

More specifically, referring to fig. 8 (a), when the pressure in the test tube is normal, the first sample segment is a segment of the sample suction port 101 (see fig. 6 (a)) of the sampling needle 1 that is far away from the sample suction port 101. When the tube is under negative pressure, the first section of the sample is the negative pressure offset and a section which is partially close to the sample suction port 101. When the pressure in the test tube is positive, the first section of the sample is a section of the sample suction port 101 of the sampling needle 1 far away from the sample suction port 101. The distance that the sample moves toward the sample suction port 101 is the same regardless of whether the inside of the test tube is at normal pressure, negative pressure, or positive pressure.

Furthermore, after the first section of sample is thrown away, the sample is divided, and no matter the pressure in the test tube is normal pressure, negative pressure or positive pressure, the distance of the sample moving to the sample suction port 101 is the same, that is, the volume of the measured sample is the same.

Furthermore, after the sample division is completed, the residual sample in the sampling channel of the sampling needle 1 is discarded. When the test tube is at normal pressure, the volume of the isolated gas column is the same as that of the isolated gas column established before sampling, so that the volume of the thrown-away end sample is the total sample suction amount minus the volume of the thrown-away first sample and the volume of the thrown-away measurement sample. When being the negative pressure in the test tube, because the volume of isolation gas post reduces for the terminal sample volume of throwing away is when the test tube is the terminal sample of throwing away when ordinary pressure and adds the volume that the isolation gas post reduces. When the test tube is in positive pressure, the volume of the isolated gas column is increased, so that the volume of the thrown-away end sample is the volume obtained by subtracting the positive pressure offset of the isolated gas column from the thrown-away end sample when the test tube is in normal pressure.

The sampling subassembly that this embodiment provided, use driving piece 2 as the syringe as the example, no matter be in the test tube for ordinary pressure, negative pressure or malleation, after sampling needle 1 withdraws from the test tube, the influence of comprehensive consideration test tube pressure to the sample in the sampling needle 1, the syringe pushes forward first preset displacement, and this first preset displacement is greater than the negative pressure offset, pushes forward the second again and predetermines the displacement and divide the appearance, later promotes forward again until all last section samples of discharging.

This embodiment sets up the first displacement of predetermineeing that the syringe promoted and is greater than the negative pressure offset, can guarantee when the test tube is the negative pressure, also can throw away partial sample at least, the phenomenon of production bubble when avoiding dividing the appearance. It will be appreciated that the present embodiment provides a sampling assembly in which the total sample volume is set to a greater amount than the normal two punctures to deflate the tube when aspirating a sample to ensure that there is an adequate measurement sample.

Preferably, considering that the pressure limit of the normal test tube is ± 30kpa, and the deformation limit of the first pipeline 15 is 0.3uL/kpa, the volume of the isolated gas column can be set up before sampling to be 12uL, and the volume of the first and last stage samples when the test tube is at normal pressure to be 12 uL. So, can satisfy and carry out a puncture and inhale kind to the deformation of different test tube pressure and first pipeline 15. It will be appreciated that the larger the volume of the column of barrier gas, the better the effect of the deformation of the first conduit 15 and the effect of the pressure in the test tube can be counteracted.

In one embodiment, referring to fig. 9, the first pipeline 15 is a rigid pipeline, so as to minimize the deformation of the first pipeline 15 when being pressed, and the sample sucking amount is controlled by the driving member 2 and the volume of the established isolation gas column offsets the sample offset in the sampling needle caused by the pressure in the test tube and the deformation of the rigid pipeline, so as to achieve the purpose of sucking the sample by one-time puncture.

In particular, since the sample of the sampling needle 1 is subjected to different pressure environments inside and outside the test tube, the sample is deviated in the sampling passage of the sampling needle 1 in relation to the test tube pressure and the deformation of the first tube 15. By arranging the deformation of the first conduit 15 as small as possible, even negligible, only the influence of the pressure in the test tube can be taken into account. Because first pipeline 15 is in the encapsulated situation when inhaling the appearance, driving piece 2 is connected to one end, other end UNICOM sampling needle 1, and sampling needle 1 inhale in the appearance mouth immerses the sample in the test tube, it is the rigid pipeline to set up first pipeline 1, thereby make the in vitro pressure can not make first pipeline 1 produce the deformation, when making sampling needle 1 the different pressure environment inside and outside the test tube, the sample of absorption can not squint in the sampling passageway of sampling needle 1, thereby realize no matter the in vitro pressure is the ordinary pressure, negative pressure or malleation, all can realize a puncture sampling.

It should be understood that the first pipeline 15 may be slightly deformed, for example, the deformation amount is less than 0.01uL/kpa, and the offset of the sample can be controlled by the volume of the isolated gas column established before sampling, the sample suction amount and the volumes of the first and last samples. For example, referring to FIG. 10, considering the pressure limit in the test tube is + -30 kpa, the volume of the isolated gas column can be set to be greater than 0.3uL, and the volume of the first and last samples in the test tube is 0.3uL under normal pressure.

Preferably, the first pipeline 15 is a steel pipe.

Since the longer the length of the first pipe 15, the more the possible minute deformation accumulates, it is preferable to set the length of the first pipe 15 as short as possible, for example, the length of the first pipe 15 is 10mm to 50 mm.

The sampling subassembly of sample analysis appearance that this application embodiment provided need not carry out twice puncture, need not release the pressure in the test tube for the ordinary pressure, the effectual sampling time that has shortened. By contrast, compared with the traditional scheme of sampling by puncturing twice, the sample analyzer of the embodiment of the application can save the sampling time from seconds to tens of seconds.

Referring to fig. 1, 7 and 9, an embodiment of the present application further provides a sampling method of a sample analyzer, the sampling method is performed based on the sample analyzer in the foregoing embodiment, the sample analyzer includes a sampling needle 1, a first pipeline 15 and a pressure regulating assembly, the sampling needle 1 is provided with a sampling channel, the first pipeline 15 is communicated with the sampling channel, the pressure regulating assembly is provided on the first pipeline and/or is communicated with the first pipeline, the sampling method includes:

the sampling needle 1 penetrates into a test tube to suck a sample, so that the sample enters a sampling channel;

the pressure regulating assembly balances or counteracts the pressure within the sampling channel.

In one embodiment, referring to fig. 1, the pressure regulating assembly further includes a first switching member 11 and a driving member 2, an end of the first pipeline 15 away from the sampling needle 1 is communicated with the driving member 2, the first switching member 11 is disposed on the first pipeline 15, and the sampling method includes:

when the sampling needle 1 pierces into the test tube, the driving member 2 drives the sampling needle 1 to suck the sample, and after the sample enters the sampling channel, the first switching member 11 cuts off the sampling channel and the driving member 2.

In one embodiment, the sampling method further comprises:

when the first switch 11 cuts off the sampling channel from the driving member 2, the driving member 2 is driven, so that the pressure of the first pipeline 15 between the first switch 11 and the driving member 2 is balanced with the outside.

In one embodiment, referring to fig. 3, the second detecting element 13 is disposed on the first pipeline 15 between the first switching element 11 and the driving element 2, and the sampling method includes:

the second detection part 13 detects the pressure of the first pipeline 15 between the first switching part 11 and the driving part 2 in real time, and the driving part 2 adjusts the pressure of the first pipeline 15 between the first switching part 11 and the driving part 2 according to the pressure detected by the second detection part 13 until the pressure of the first pipeline 15 is balanced with the outside.

In one embodiment, referring to fig. 1, the sampling assembly further includes a first detecting member 12, the first detecting member 12 is disposed on the first pipeline 15, and the sampling method further includes:

when the first detecting part 12 detects that the liquid column height of the sample in the sampling channel reaches the preset height, the first switching part 11 cuts off the sampling needle 1 and the driving part 2.

In one embodiment, the sample analyzer comprises a reservoir 3, the reservoir 3 is connected to the driving member 2, and the sampling method comprises:

the reservoir 3 communicates with the driver 2 for releasing the pressure in the first line 15 between the first switch member 11 and the driver 2.

In one embodiment, the sampling assembly further comprises a constant pressure source 35, the constant pressure source 35 being connected to the reservoir 3 and adapted to provide a constant pressure to the reservoir 3,

when pressure needs to be released from the first pipeline 15 between the first switching piece 11 and the driving piece 2, the liquid storage tank 3 is arranged to be communicated with the driving piece 2, and the normal pressure source 35 is communicated with the liquid storage tank 3.

In one embodiment, a second switch 21 is provided between the driving member 2 and the reservoir 3, and when pressure needs to be released from the first pipeline 15 between the first switch 11 and the driving member 2, the sampling method includes:

the second switching member 21 communicates the driving member 2 with the reservoir 3.

In one embodiment, the sampling assembly further comprises a positive pressure source 34, the positive pressure source 34 being connected to the reservoir 3 for pressurizing the reservoir 3, the sampling method comprising:

after sampling needle 1 divides the appearance to accomplish, malleation source 34 is to liquid storage tank 3 pressure boost, and second switching piece 21 intercommunication driving piece 2 and liquid storage tank 3, first switching piece 11 intercommunication sampling needle 1 and driving piece 2 for the liquid inflow driving piece 2 of liquid storage tank 3, and in the sampling passageway of sampling needle 1 is flowed into through first pipeline 15, wash the sampling passageway.

In one embodiment, a third switch 32 is provided between the positive pressure source 34, the normal pressure source 35 and the liquid storage tank 3, and the sampling method includes:

when the sampling channel needs to be cleaned, the third switching piece 32 is communicated with the positive pressure source 34 and the liquid storage tank 3; and/or when pressure needs to be relieved to the first line 15 between the first switch member 11 and the drive member 2, the third switch member 32 connects the pressure source 35 to the reservoir 3.

In one embodiment, the sampling assembly further comprises a reagent barrel 4 and a negative pressure source 33, the reagent barrel 4 and the negative pressure source 33 are both connected with the liquid storage tank 3, the negative pressure source 33 is used for reducing the pressure of the liquid storage tank 3, and the sampling method further comprises:

when the liquid storage tank 3 needs to be filled, the liquid storage tank 3 is communicated with the negative pressure source 33 and the reagent barrel 4, and liquid in the reagent barrel 4 flows into the liquid storage tank 3 under the action of pressure difference.

In one embodiment, referring to fig. 5, the sampling assembly further includes a swab 5, the swab 5 includes an atmosphere end, a second three-way joint 16 is disposed on the first pipeline 15 between the first switching element 11 and the driving element 2, and a sixth switching element 17 is disposed between the atmosphere end of the swab 5 and the second three-way joint 16, and the sampling method further includes:

when pressure needs to be released to the first conduit 15 between the first switch 11 and the actuating member 2, the sixth switch 17 communicates the second three-way connection 16 with the atmosphere end of the swab 5.

In one embodiment, a third three-way joint 52 is further disposed between the sixth switching member 17 and the atmosphere end of the swab 5, a fourth three-way joint 23 is further disposed between the second switching member 21 and the liquid storage tank 3, and a seventh switching member 53 is further disposed between the third three-way joint 52 and the fourth three-way joint 23, and the sampling method further includes:

when the pressure in the first pipeline 15 between the first switching element 11 and the driving element 2 is released, the sixth switching element 17 cuts off the second three-way joint 16 and the third three-way joint 52, the second switching element 21 cuts off the fourth three-way joint 23 and the driving element 2, the seventh switching element 53 communicates the third three-way joint 52 and the fourth three-way joint 23, and the liquid in the liquid storage tank 3 is introduced into the atmosphere end of the swab 5 to clean the outer wall of the sampling needle 1.

In one embodiment, referring to fig. 7, the sampling assembly further includes a driving member 2, the driving member 2 is connected to an end of the first pipeline 15 away from the sampling needle 1, the first pipeline 15 has a deformation capability, the pressure regulating assembly is the driving member 2, and the sampling method includes:

the driving member 2 establishes an isolating gas column in the sampling needle 1 before sampling, and controls the sample suction amount of the sampling needle 1 to counteract the offset influence of the pressure in the test tube and the deformation of the first pipeline 15 on the sample in the sampling needle 1.

Preferably, the volume of the barrier gas column is not less than 12 uL.

In one embodiment, referring to fig. 9, the sampling assembly further includes a driving member 2, the driving member 2 is connected to an end of the first pipeline 15 away from the sampling needle 1, the first pipeline 15 is a rigid pipeline, the pressure regulating assembly is the driving member 2, and the sampling method includes:

the driving part 2 establishes an isolation air column before sampling, and controls the sample suction amount of the sampling needle 1 to counteract the offset influence of the pressure in the test tube on the sample in the sampling needle 1.

Preferably, the first pipeline 15 is a steel pipe, and the length of the first pipeline 15 is 10mm to 50 mm.

While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.