阅读说明：本技术 一种血液中总rna提取试剂盒及其使用方法 (Kit for extracting total RNA from blood and use method thereof ) 是由 赵杰 何贤英 颜林林 薛方方 崔芳芳 薛茹月 李砺锋 于 2021-09-17 设计创作，主要内容包括：本发明提供一种血液中总RNA提取试剂盒及其使用方法,有效的解决了现有的试剂盒不能够根据不同大小试管进行调节和不具有做离心实验的功能的问题,所述定位盘上均匀固定安装有多个竖板,每个所述竖板上分别上下滑动安装有弧形架,每个所述弧形架上分别转动安装有夹持装置,所述定位盘内均匀上下滑动安装有多个与每个所述夹持装置对应的滑动圆板,每个所述滑动圆板圆周面的上端与定位盘之间分别固定安装有多个内压簧,所述试剂盒内固定安装有位于定位盘下方的限位盘,每个所述滑动圆板上分别安装有位于每个所述夹持装置内的试管,转动架、顶块和推动连杆之间的配合使用,可以对每个试管震荡混匀,进一步减轻了工作人员的工作强度。(The invention provides a kit for extracting total RNA from blood and a using method thereof, which effectively solve the problems that the existing kit can not be adjusted according to test tubes with different sizes and does not have the function of centrifugal experiment, a plurality of vertical plates are uniformly and fixedly arranged on a positioning disc, arc frames are respectively arranged on each vertical plate in a vertical sliding manner, a clamping device is respectively and rotatably arranged on each arc frame, a plurality of sliding circular plates corresponding to each clamping device are uniformly and vertically arranged in the positioning disc in a sliding manner, a plurality of internal pressure springs are respectively and fixedly arranged between the upper end of the circumferential surface of each sliding circular plate and the positioning disc, a limiting disc positioned below the positioning disc is fixedly arranged in a reagent box, a test tube positioned in each clamping device is respectively arranged on each sliding circular plate, and the rotating frame, a top block and a pushing connecting rod are matched for use, can vibrate the mixing to every test tube, further alleviate staff's working strength.)

1. A kit for extracting total RNA from blood comprises a kit (1) and is characterized in that: a positioning disc (2) is fixedly arranged in the kit (1), a plurality of vertical plates (3) are uniformly and fixedly arranged on the positioning disc (2), arc-shaped frames (4) are respectively arranged on the vertical plates (3) in a vertical sliding manner, clamping devices are respectively rotatably arranged on the arc-shaped frames (4), a plurality of sliding circular plates (5) corresponding to each clamping device are uniformly and vertically arranged in the positioning disc (2) in a sliding manner, a plurality of inner pressure springs (6) are respectively and fixedly arranged between the upper end of the circumferential surface of each sliding circular plate (5) and the positioning disc (2), a limiting disc (7) positioned below the positioning disc (2) is fixedly arranged in the kit (1), the upper end of limiting plate (7) slidable mounting about respectively has a plurality ofly and every push rod (8) that slip plectane (5) correspond, every install respectively on slip plectane (5) and be located every test tube (9) in the clamping device.

2. The kit for extracting total RNA from blood according to claim 1, wherein: even fixed mounting has a plurality of being located every on spacing dish (7) riser (1) (0) of push rod (8) one side, every about sliding mounting is on riser (10) respectively, every between the bottom of push rod (8) and spacing dish (7) be connected with down extension spring (11), every respectively sliding mounting have with every on spacing dish (7) push rod (8) articulated push connecting rod (12), every rotate respectively on push connecting rod (12) and install kicking block (13) that are close to spacing dish (7) center department, rotating turret (14) are installed to the up end coaxial rotation of spacing dish (7), on rotating turret (14) respectively fixed mounting have a plurality ofly with lug (15) of every kicking block (13) sliding contact.

3. The kit for extracting total RNA from blood according to claim 1, wherein: every clamping device rotates respectively and installs every first arc circle (2) (1), every on arc frame (4) the upper end of first arc circle (21) fixed mounting respectively has first semi-gear (16), every rotate respectively on arc frame (4) and install second arc circle (22) that are located first arc circle (21) one side, every on second arc circle (22) respectively fixed mounting have second semi-gear (17), every the one end of first arc circle (21) rotates respectively and installs and screw thread install adjusting screw (18) on first arc circle (21), every on adjusting screw (18) respectively fixed mounting have regulating wheel (19), every install guide bar (20) between the other end of first arc circle (21) and first arc circle (21) respectively.

4. The kit for extracting total RNA from blood according to claim 3, wherein: a plurality of arc-shaped pressure springs (23) are fixedly mounted on the surfaces, close to each other, of each first half gear (16) and each second half gear (17) respectively, arc-shaped clamping rings (24) are fixedly mounted between the arc-shaped pressure springs (23) located on the same side respectively, and rotating balls (25) tightly attached to test tubes (9) are rotatably mounted on each arc-shaped clamping ring (24) respectively.

5. The kit for extracting total RNA from blood according to claim 1, wherein: reagent box lid (26) is installed in the rotation on kit (1), reagent box lid (26) lower terminal surface respectively fixed mounting have a plurality ofly with every pressure spring (27) that test tube (9) correspond, every pressure spring (27) lower extreme respectively fixed mounting have be located every test tube (9) upper port's push pedal (28), every the lower extreme respectively fixed mounting of push pedal (28) has and is located every rubber stopper (29) in test tube (9).

6. The kit for extracting total RNA from blood according to claim 4, wherein: every the upper end of arc frame (4) rotates respectively install with every driven gear (30) of first semi-gear (16) meshing, it is a plurality of install between driven gear (30) and be located the band pulley group (31) of arc frame (4) lower extreme, one of them the lower extreme of arc frame (4) fixed mounting respectively has with band pulley group (31) coaxial fixed starter motor, every in riser (3) fixed mounting respectively have and be located reset spring (33) of arc frame (4) lower extreme.

7. The kit for extracting total RNA from blood according to claim 2, wherein: the utility model discloses a test tube kit, including reagent box (1), bottom fixed mounting of reagent box (1) has and runs through dead lever (34) of positioning disk (2) and spacing dish (7), coaxial fixed mounting has ring gear (35) on rotating turret (14), fixed mounting has beating motor (36) with gear (35) meshing on dead lever (34), slidable mounting has and runs through every from top to bottom in reagent box (1) bearing board (37) of test tube (9), needle tubing (38) have been placed to the upper end of bearing board (37).

8. The use method of the kit for extracting total RNA from blood according to claim 1, wherein the kit comprises:

the first step is as follows: opening the reagent box cover (26) and taking out the carrier plate (37);

the second step is that: then, putting each test tube (9) into each clamping device respectively, and covering a bearing plate (37);

the third step: then, a needle tube (38) is used for adding corresponding liquid into each test tube (9);

the fourth step: then starting a striking motor (36) to vibrate each test tube (9);

the fifth step: then starting a starting motor to perform centrifugal operation corresponding to each test tube (9);

and a sixth step: subsequently removing each test tube (9);

the seventh step: finally, the liquid in each test tube (9) is extracted by using a needle tube (38).

Technical Field

The invention relates to the field of RNA extraction, in particular to a total RNA extraction kit in blood and a using method thereof.

Background

Ribonucleic acids (abbreviated as RNA), genetic information carriers present in biological cells and in parts of viruses, viroids. RNA is a long chain molecule formed by the condensation of ribonucleotides via phosphodiester bonds. One ribonucleotide molecule consists of a phosphate, a ribose and a base. RNA has 4 main bases, namely A (adenine), G (guanine), C (cytosine) and U (uracil), wherein U (uracil) replaces T in DNA. The role of ribonucleic acids in the body is primarily to direct protein synthesis.

When RNA extraction is carried out, a corresponding kit is needed, but the existing kit has certain disadvantages in use:

1. although a supporting plate is arranged in the existing kit and used for placing test tubes, the supporting plate cannot be adjusted according to test tubes of different sizes, so that the use is limited;

2. the existing kit does not have the function of centrifugal experiment, which causes the slow progress of the work of workers and influences the work efficiency.

3. The existing kit does not have the function of shaking and mixing uniformly, so that the problem of low efficiency is caused.

Therefore, the invention provides a blood total RNA extraction kit and a use method thereof to solve the problem.

Disclosure of Invention

Aiming at the situation and overcoming the defects of the prior art, the invention provides the total RNA extraction kit in blood and the using method thereof, which effectively solve the problems that the existing kit can not be adjusted according to test tubes with different sizes and does not have the function of centrifugal experiment.

The utility model provides a total RNA draws kit in blood, includes the kit, fixed mounting has the positioning disk in the reagent box, even fixed mounting has a plurality of risers on the positioning disk, every slidable mounting has arc frame, every about respectively on the riser rotate respectively on the arc frame and install clamping device, slidable mounting has a plurality ofly and every about evenly in the positioning disk the slip plectane that clamping device corresponds, every fixed mounting has a plurality of interior pressure springs respectively between the upper end of slip plectane periphery and the positioning disk, fixed mounting has the spacing dish that is located the positioning disk below in the reagent box, slidable mounting has a plurality ofly and every about respectively on the upper end of limiting plate the push rod that the slip plectane corresponds, every install respectively on the slip plectane and be located every test tube in the clamping device.

Preferably, even fixed mounting has a plurality of risers that are located every on the spacing dish the riser of push rod one side, every slidable mounting is on the riser about the push rod is respectively, every be connected with down the extension spring between the bottom of push rod and the spacing dish respectively, every slidable mounting has and every on the spacing dish respectively the articulated promotion connecting rod of push rod, every rotate respectively on the promotion connecting rod and install the kicking block that is close to spacing dish center department, the rolling stock is installed in the up end coaxial rotation of spacing dish, fixed mounting has a plurality ofly and every kicking block sliding contact's lug on the rolling stock respectively.

Preferably, every clamping device is including rotating respectively and installing every first arc circle on the arc frame, every the upper end of first arc circle fixed mounting respectively has first half gear, every rotate respectively on the arc frame and install the second arc circle that is located first arc circle one side, every there is second half gear, every fixed mounting respectively on the second arc circle respectively the adjusting screw who installs just the thread on first arc circle is rotated respectively to the one end of first arc circle, every there is the regulating wheel, every fixed mounting respectively on the adjusting screw the guide bar is installed respectively between the other end of first arc circle and the first arc circle.

Preferably, a plurality of arc-shaped pressure springs are fixedly mounted on the surfaces, close to each other, of each first half gear and each second half gear respectively, arc-shaped clamping rings are fixedly mounted between the arc-shaped pressure springs located on the same side respectively, and a rotating ball tightly attached to a test tube is rotatably mounted on each arc-shaped clamping ring respectively.

Preferably, the reagent box is provided with a reagent box cover in a rotating manner, the lower end face of the reagent box cover is respectively and fixedly provided with a plurality of pressure springs corresponding to the test tubes, the lower ends of the pressure springs are respectively and fixedly provided with a push plate positioned at the upper port of each test tube, and the lower ends of the push plates are respectively and fixedly provided with rubber plugs positioned in the test tubes.

Preferably, every the upper end of arc frame is rotated respectively install with every driven gear that first semi-gear meshes is a plurality of install the band pulley group that is located the arc frame lower extreme between the driven gear, one of them the lower extreme of arc frame fixed mounting respectively have with the coaxial fixed starter motor of band pulley group, every respectively fixed mounting has the reset spring that is located the arc frame lower extreme in the riser.

Preferably, the bottom fixed mounting of kit has and runs through the dead lever of positioning disk and spacing dish, coaxial fixed mounting has the ring gear on the rotating turret, fixed mounting has the hitting motor with gear engagement on the dead lever, slidable mounting has and runs through every from top to bottom in the kit the loading board of test tube, the needle tubing has been placed to the upper end of loading board.

A method for using a total RNA extraction kit in blood comprises the following steps:

the first step is as follows: opening the reagent box cover, and taking out the bearing plate;

the second step is that: then, putting each test tube into each clamping device respectively, and covering the bearing plate;

the third step: then, the needle tube 38 is used to add the corresponding liquid to each test tube;

the fourth step: starting a beating motor, and oscillating each test tube;

the fifth step: then starting a starting motor to perform centrifugal operation on each test tube;

and a sixth step: then taking out each test tube;

the seventh step: finally, the liquid in each test tube is respectively extracted by using a needle tube.

The invention has the beneficial effects that:

1. through the cooperation use between test tube, clamping device and the slip plectane, can vibrate the mixing to every test tube, corresponding working strength who alleviates the staff improves work efficiency.

2. Through the cooperation use between clamping device and the pulley group, carry out the centrifugation experiment to the liquid in every test tube, solved the problem of current kit function singleness, further improved efficiency.

3. The cooperation between rotating turret, kicking block and the promotion connecting rod is used, can vibrate the mixing to every test tube, has further alleviateed staff's working strength.

Drawings

Fig. 1 is a perspective view of the present invention.

Fig. 2 is a view of the pulley set installation of the present invention.

Fig. 3 is a view showing the installation of the inner spring according to the present invention.

Fig. 4 is a view showing the installation of the ring gear of the present invention.

FIG. 5 is an enlarged view of the invention at A.

FIG. 6 is a view of the arcuate clamp ring installation of the present invention.

FIG. 7 is a view showing the installation of the rotating ball of the present invention.

Fig. 8 is an installation view of the arc-shaped frame of the present invention.

FIG. 9 is a view showing the tube installation of the present invention.

Fig. 10 is a driven gear mounting diagram of the present invention.

Detailed Description

The foregoing and other aspects, features and advantages of the invention will be apparent from the following more particular description of embodiments of the invention, as illustrated in the accompanying drawings in which reference is made to figures 1 to 10. The structural contents mentioned in the following embodiments are all referred to the attached drawings of the specification.

Exemplary embodiments of the present invention will be described below with reference to the accompanying drawings.

In the first embodiment, as shown in fig. 1 to 4, a kit for extracting total RNA from blood includes a kit 1, the kit 1 is of a circular tube structure, a positioning plate 2 is fixedly installed in the kit 1, a plurality of vertical plates 3 are fixedly installed on the uniform circumference of the upper end surface of the positioning plate 2, a vertical groove is respectively penetrated between the left side and the right side of each vertical plate 3, an arc-shaped frame 4 located in the vertical groove is respectively installed on each vertical plate 3 in a vertically sliding manner, a clamping device is respectively installed on each arc-shaped frame 4 in a rotating manner, and a plurality of sliding grooves are respectively and uniformly formed on the positioning plate 12;

the positioning disc 2 is respectively provided with a sliding circular plate 5 positioned in each sliding groove in an up-and-down sliding manner, each sliding circular plate 5 corresponds to each clamping device, a plurality of inner pressure springs 6 are fixedly arranged between the upper end surface of the circumference of each sliding circular plate 5 and the positioning disc 2 respectively, a limiting disc 7 positioned below the positioning disc 2 is fixedly arranged in the kit 1, the upper end of the limiting plate 7 is respectively provided with a plurality of push rods 8 corresponding to each sliding circular plate 5 in an up-and-down sliding manner, rubber blocks are fixedly arranged at the upper ends of the push rods 8 respectively, test tubes 9 positioned in each clamping device are arranged on each sliding circular plate 5 respectively, and each test tube 9 is suitable for the existing test tube respectively;

when the working personnel needs to use the device, the corresponding liquid is respectively added into each test tube 9, then after each test tube 9 is prepared, each test tube 9 is respectively placed into the corresponding clamping device, the bottom of each test tube 9 is respectively attached to the upper end of the sliding circular plate 5, then each push rod 8 is respectively pushed by the corresponding device to slide up and down, each rubber block is respectively pushed against the corresponding sliding circular plate 5 to slide up, the damage degree to the sliding circular plate 5 can be reduced due to the arrangement of each rubber block, at the moment, each sliding circular plate 5 can respectively press the corresponding inner pressure spring 6 while sliding up and down, and can also push the corresponding test tube 9 to slide up and down, meanwhile, each clamping device can slide up and down, each test tube 9 is vibrated and uniformly mixed to decompose blood cells in the test tube 9, and then each sliding circular plate 5 is continuously pushed to slide up and down, at this moment, the test tubes 9 slide up and down, vibrate the mixing to every test tube 9, make nucleic acid protein complex separate completely, through the cooperation between above-mentioned each part use, can alleviate staff's working strength to every test tube 9's vibration degree, improve work efficiency.

In the second embodiment, on the basis of the first embodiment, as shown in fig. 4-5, a plurality of vertical plates 10 located on one side of each push rod 8 are uniformly and fixedly installed at the upper end of the limiting disc 7, each vertical plate 10 is respectively provided with a sliding cavity, each push rod 8 is respectively installed in the sliding cavity of each vertical plate 10 in a vertical sliding manner, a lower tension spring 11 is respectively connected between the bottom of each push rod 8 and the limiting disc 7, each limiting disc 7 is respectively provided with a strip-shaped groove corresponding to each vertical plate 10, each push rod 8 is respectively hinged with a push connecting rod 12 slidably installed in each strip-shaped groove, each push connecting rod 12 is respectively rotatably installed with an ejector block 13 which is close to the center of the limiting disc 7 and is also slidably installed on the limiting disc 7, the outer surface of each ejector block 13 is a round surface, and a rotating frame 14 is coaxially and rotatably installed on the upper end surface of the limiting disc 7, a plurality of convex blocks 15 in sliding contact with each top block 13 are fixedly arranged on the circumferential side surface of the rotating frame 14 respectively;

when each test tube 9 needs to be oscillated, the rotating frame 14 is pushed to rotate, then the rotating frame 14 drives each bump 15 to rotate, when each bump 15 is respectively contacted with the corresponding top block 13, each bump 15 respectively pushes the corresponding top block 13 to approach to the corresponding vertical plate 10, then each pushing connecting rod 12 respectively slides, then each pushing connecting rod 12 respectively pushes the corresponding push rod 8 to tightly cling to the corresponding vertical plate 10 to slide upwards, and simultaneously each lower tension spring 11 is stretched, and then each push rod 8 respectively pushes the sliding circular plate 5 through each rubber block to slide upwards;

when every lug 15 breaks away from corresponding kicking block 13, because extension spring 11 reset effect under every, this moment every push rod 8 resets, simultaneously because the reason of extension spring 6 in every, every test tube 9 lapse this moment, through constantly sliding from top to bottom like this, can vibrate the mixing to every test tube 9, further alleviateed staff's working strength.

In a third embodiment, on the basis of the first embodiment, as shown in fig. 6 to 7, each of the clamping devices includes a first arc ring 21 rotatably installed on each of the arc frames 4, each of the first arc rings 21 is rotatable on the arc frame 4, a first half gear 16 is fixedly installed at an upper end of each of the first arc rings 21, a second arc ring 22 located on one side of the first arc ring 21 is rotatably installed on each of the arc frames 4, each of the second arc rings 22 is rotatable on the arc frame 4, a second half gear 17 corresponding to each of the first half gears 16 is fixedly installed on each of the second arc rings 22, an adjusting screw 18 is rotatably installed at one end of each of the first arc rings 21 and threadedly installed on the first arc ring 21, an adjusting wheel 19 is fixedly installed on each of the adjusting screws 18, a guide rod 20 is respectively arranged between the other end of each first arc-shaped ring 21 and the first arc-shaped ring 21;

in use, each adjusting wheel 19 is rotated, each adjusting wheel 19 then carries the adjusting screw 18 to rotate, each second half gear 17 is far away from the first half gear 16 due to the rotation of each adjusting screw 19, the second half gears 17 respectively slide on the corresponding guide rods 20, after the distance between each first half gear 16 and each second half gear 17 is adjusted, a worker then puts each test tube 9 between each first half gear 16 and each second half gear 17 respectively, the worker then rotates each adjusting wheel 19 reversely, each adjusting wheel 19 then carries the corresponding adjusting screw 18 respectively, each adjusting screw 18 then carries the corresponding second half gear 17 to approach the corresponding first half gear 16 respectively in a threaded manner, and simultaneously clamps the test tubes 9 which are adjacent to each other, so that the requirements of the test tubes 9 of different models can be met, compared with single operation, the working efficiency is improved.

In the fourth embodiment, on the basis of the third embodiment, as shown in fig. 7, a plurality of arc-shaped pressure springs 23 are respectively and fixedly mounted on surfaces of each first half gear 16 and each second half gear 17, which are close to each other, arc-shaped clamping rings 24 are respectively and fixedly mounted between the plurality of arc-shaped pressure springs 23 located on the same side, a plurality of rotating beads 25 tightly attached to the test tube 9 are respectively and rotatably mounted on each arc-shaped clamping ring 24, and each rotating bead 25 is made of a plastic material, so that the test tube 9 is prevented from being damaged;

in use, each adjustment wheel 19 then rotates with the adjustment screw 18, each second half-gear 17 then moves away from the first half-gear 16 as a result of the rotation of each adjustment screw 19, while each second half-gear 17 slides on the corresponding guide bar 20, when the distance between each first half-gear 16 and each second half-gear 17 has been adjusted, the operator then places each test tube 9 between each two arc-shaped clamping rings 24, and then the operator then rotates each adjustment wheel 19 in the opposite direction, while each adjustment wheel 19 carries with it the corresponding adjustment screw 18, each adjustment screw 18 then threads with it the corresponding second half-gear 17 towards the corresponding first half-gear 16, and then the second half-gear 17 and the first half-gear 16 push against each arc-shaped clamping ring 24, until each rotating bead 25 abuts against the outer wall of the corresponding test tube 9, simultaneously every arc spring 23 is bounce-back respectively to corresponding arc clamp ring 24, prevents that test tube 9 from appearing not hard up when using, also uses not equidimension test tube 9 simultaneously, has improved work efficiency.

Fifth embodiment, on the basis of the first embodiment, as shown in fig. 1, a reagent box cover 26 is rotatably mounted on the reagent box 1, an existing self-made component is disposed between the reagent box cover 26 and the reagent box to prevent the reagent box cover 26 from opening when in use, a plurality of pressure springs 27 corresponding to each test tube 9 are fixedly mounted on the lower end surface of the reagent box cover 26, a push plate 28 located at an upper port of each test tube 9 is fixedly mounted on the lower end of each pressure spring 27, and a rubber plug 29 located in each test tube 9 is fixedly mounted on the lower end of each push plate 28;

after each test tube 9 is prepared, each test tube 9 is respectively placed into the corresponding clamping device, the bottom of each test tube 9 is respectively attached to the upper end of the sliding circular plate 5, then each push rod 8 is respectively pushed by the corresponding device to slide up and down, each rubber block respectively pushes the bottom of the corresponding sliding circular plate 5 to slide up and down, the arrangement of each rubber block can reduce the damage degree to the sliding circular plate 5, at the moment, each sliding circular plate 5 can respectively press the corresponding inner compression spring 6 while sliding up and down, and simultaneously, each test tube 9 can also be pushed to slide up and down, at the moment, each clamping device also slides up and down, when each test tube 9 respectively slides up, each test tube 9 respectively pushes the push plate 28 and the rubber plug 29 to slide up, and the arrangement of the rubber plug can prevent the liquid in the test tube 9 from flowing out due to vibration, meanwhile, each push plate 28 pushes the corresponding pressure spring 27 to compress, when each test tube 9 slides downwards, each push plate 28 and the rubber plug 29 slide downwards along with the corresponding test tube 9 due to the resilience of each pressure spring 27, each test tube 9 is protected, and through the matching use among the parts, the vibration degree of workers to each test tube 9 can be reduced, the working efficiency is improved, and meanwhile, liquid in the test tube 9 is prevented from flowing out.

Sixth embodiment, on the basis of the fourth embodiment, as shown in fig. 2, a driven gear 30 engaged with each first half gear 16 is rotatably mounted at the upper end of each arc-shaped frame 4, a pulley set 31 located at the lower end of the arc-shaped frame 4 is mounted between a plurality of driven gears 30, the belt pulley set 31 is composed of a plurality of belt pulleys and belts, the lower end of each arc-shaped frame 4 is rotatably provided with a belt pulley which is coaxially and fixedly connected with each driven gear 30, the belts are arranged among the plurality of belt pulleys, the lower end of one of the arc-shaped frames 4 is respectively and fixedly provided with a starting motor which is coaxially fixed with the belt wheel set 31, the starting motor is connected with a controller and a power supply, a reset spring 33 positioned at the lower end of the arc-shaped frame 4 is fixedly arranged in a vertical groove of each vertical plate 3 respectively, and the upper end of each reset spring 33 is fixedly connected to the corresponding arc-shaped frame 4 respectively;

when each test tube 9 is centrifuged, the starting motor is controlled to be started, then the starting motor drives each driven gear 30 to rotate through the belt pulley set, then each driven gear 30 is meshed with the corresponding first half gear 16 to rotate, as shown in fig. 6, each first half gear 16 and the corresponding second half gear 17 are mutually attached, each first half gear 16 drives the corresponding arc-shaped clamping ring 24 to rotate on the arc-shaped frame 4, then the arc-shaped clamping ring 24 on the lower end of the second half gear 17 also rotates on the arc-shaped frame 4, each first half gear 16 drives the corresponding second half gear 17 to rotate on the corresponding arc-shaped frame 4, each test tube 9 is driven to rotate through the corresponding component, the liquid in each test tube 9 is centrifuged, and the specific centrifugation time is carried out according to the corresponding requirement, after the centrifugal experiment is completed, the liquid in each test tube 9 can be taken out, and then the corresponding separation experiment is carried out, so that the problem of single function of the existing kit is solved, and the efficiency is further improved.

Seventh embodiment, on the basis of the second embodiment, as shown in fig. 4, a fixing rod 34 is fixedly installed at the bottom of the reagent kit 1 and penetrates through the positioning disk 2 and the limiting disk 7, the rotating frame 14 is coaxially and rotatably installed on the fixing rod 34, a toothed ring 35 is coaxially and fixedly installed on the rotating frame 14, a striking motor 36 engaged with the toothed ring 35 is fixedly installed on the fixing rod 34, the striking motor 36 is connected with a controller and a power source, a motor gear engaged with the toothed ring 35 is coaxially and fixedly installed on a rotating shaft of the striking motor 36, the striking motor 36 can drive the toothed ring 35 to rotate when in use, then the toothed ring 35 drives the rotating frame 14 to rotate, and then the rotating frame 14 drives the plurality of protruding blocks 15 to rotate;

the kit is characterized in that the kit 1 is internally provided with a bearing plate 37 which vertically slides and penetrates through each test tube 9, the upper end of the inner surface of the kit 1 is respectively provided with a limiting groove positioned above each vertical plate 3, the bearing plate 37 is fixedly provided with a clamping plate which vertically slides in the limiting groove, the bearing plate 37 can be taken out when in use, the upper end of the bearing plate 37 is provided with a needle tube 38, and the needle tube 38 can be used for adding corresponding liquid into each test tube 9 through the needle tube 38, so that the operation of a worker is facilitated.

Example eight, a method for using a total RNA extraction kit in blood:

the first step is as follows: firstly, checking whether the device can be used normally, then opening the reagent box cover 26, and then taking out the bearing plate 37;

the second step is that: then each test tube 9 is placed into each holding device, and the bearing plate 37 is covered;

the third step: then, the needle tube 38 is used to add the corresponding liquid to each test tube 9;

the fourth step: then starting a striking motor 36 to vibrate each test tube 9, and after the vibration is finished, closing the striking motor 36;

the fifth step: then starting a starting motor to perform centrifugal operation corresponding to each test tube 9, and after the centrifugation is finished, checking whether the centrifugation is qualified;

and a sixth step: then taking out each test tube 9, and putting each test tube 9 into a corresponding place;

the seventh step: finally, the liquid in each test tube 9 is extracted by using the needle tube 38, and the experiment is ended.

The invention has the beneficial effects that:

1. through the cooperation use between test tube, clamping device and the slip plectane, can vibrate the mixing to every test tube, corresponding working strength who alleviates the staff improves work efficiency.

2. Through the cooperation use between clamping device and the pulley group, carry out the centrifugation experiment to the liquid in every test tube, solved the problem of current kit function singleness, further improved efficiency.

3. The cooperation between rotating turret, kicking block and the promotion connecting rod is used, can vibrate the mixing to every test tube, has further alleviateed staff's working strength.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.