阅读说明：本技术 一种抗氧化虾青素乳液及其制备方法 (Antioxidant astaxanthin emulsion and preparation method thereof ) 是由 宋永先 于 2021-08-23 设计创作，主要内容包括：本申请涉及化妆品技术领域,更具体地说,它涉及一种抗氧化虾青素乳液及其制备方法。一种抗氧化虾青素乳液,按重量百分比计算,由如下组分组成：皮肤调理剂、抗氧化剂、甘油、丁二醇、丙烯酸(酯)类/C10-30烷醇丙烯酸酯交联聚合物、甘油聚醚-26、丁二醇椰油酸酯、乙基纤维素、环五聚二甲基硅氧烷、辛酸/癸酸甘油三酯、C10-18脂酸甘油三酯类、生物糖胶-1、乙基己基甘油、香精、去离子水；其中,所述抗氧化剂由虾青素、生育酚乙酸酯与茯苓提取物混合组成。本申请的抗氧化虾青素乳液中,虾青素、生育酚乙酸酯与茯苓提取物有良好的协同增效作用,可有效的提高对皮肤的抗氧化功效,对衰老皮肤进行修复,提高衰老皮肤的弹性。(The application relates to the technical field of cosmetics, in particular to an antioxidant astaxanthin emulsion and a preparation method thereof. The antioxidant astaxanthin emulsion comprises the following components in percentage by weight: skin conditioning agent, antioxidant, glycerol, butanediol, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, glyceryl polyether-26, butanediol cocoate, ethyl cellulose, cyclopentadimethylsiloxane, caprylic/capric triglyceride, C10-18 fatty acid triglyceride, bioglycan-1, ethylhexyl glycerol, essence and deionized water; wherein the antioxidant is prepared by mixing astaxanthin, tocopherol acetate and Poria extract. In the antioxidant astaxanthin emulsion, the astaxanthin, the tocopherol acetate and the poria cocos extract have good synergistic effect, so that the antioxidant effect on the skin can be effectively improved, the aged skin can be repaired, and the elasticity of the aged skin can be improved.)

1. The antioxidant astaxanthin emulsion is characterized by comprising the following components in percentage by weight:

0.45-0.55% of skin conditioner;

0.1 to 0.4 percent of antioxidant;

0.02-0.05% of chelating agent;

0.12-0.15% of preservative;

0.18 to 0.2 percent of pH regulator;

3-5% of glycerol;

2.0 to 3.0 percent of butanediol;

acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer 0.15-0.25%;

0.15-0.25% of xanthan gum;

261.5-2.5% of glyceryl polyether;

isostearyl alcohol 0.60-0.65%;

0.60 to 0.65 percent of butanediol cocoate;

0.60 to 0.65 percent of ethyl cellulose;

0.8 to 1.2 percent of cyclopentasiloxane;

caprylic/capric triglyceride 4.0-5.0%;

0.8-1.2% of C10-18 fatty acid triglyceride;

10.2 to 0.3 percent of biological carbohydrate gum;

0.45 to 0.50 percent of ethylhexyl glycerol;

0.02-0.05% of essence;

the balance of deionized water;

wherein the antioxidant is prepared by mixing astaxanthin, tocopherol acetate and Poria extract.

2. The antioxidant astaxanthin emulsion of claim 1, consisting of, in weight percent:

0.47-0.51% of skin conditioner;

0.16-0.28% of antioxidant;

chelating agent 0.026-0.038%;

0.13 to 0.14 percent of preservative;

0.18 to 0.19 percent of pH regulator;

3.4 to 4.2 percent of glycerin;

2.20 to 2.60 percent of butanediol;

acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer 0.17-0.21%;

0.17-0.21% of xanthan gum;

glyceryl polyether-261.70-2.21%;

isostearyl alcohol 0.61-0.63%;

0.61-0.63% of butanediol cocoate;

0.61-0.63% of ethyl cellulose;

0.88 to 1.04 percent of cyclopentadimethylsiloxane;

caprylic/capric triglyceride 4.20-4.60%;

0.888-1.04% of C10-18 fatty acid triglyceride;

10.22 to 0.26 percent of biological carbohydrate gum;

0.46 to 0.48 percent of ethylhexyl glycerol;

0.03-0.04% of essence;

the balance of deionized water;

wherein the antioxidant is prepared by mixing astaxanthin, tocopherol acetate and Poria extract;

the chelating agent is EDTA disodium;

the preservative is methyl hydroxybenzoate.

3. The antioxidant astaxanthin emulsion of claim 1, wherein the antioxidant is composed of astaxanthin, tocopherol acetate and Poria cocos wolf extract mixed in a weight ratio of 1 (0.8-1.2) to (1.0-1.4).

4. The antioxidant astaxanthin emulsion of claim 3, wherein the astaxanthin is astaxanthin liposomes prepared by: firstly stirring and mixing cholesterol, lecithin and ethanol, then adding astaxanthin, propylene glycol and deionized water, stirring and mixing, and then sequentially shearing and homogenizing to obtain the astaxanthin nano liposome.

5. The antioxidant astaxanthin emulsion of claim 4, wherein the lecithin is any one of egg yolk lecithin and soybean lecithin.

6. The emulsion of astaxanthin according to claim 4, wherein the mixing temperature of the lecithin, the cholesterol and the ethanol is 45-55 ℃ and the shearing time is 15-25min during the preparation of the astaxanthin liposome; the homogenizing pressure is 125-135 MPa.

7. The antioxidant astaxanthin emulsion of claim 1, wherein the skin conditioning agent is one or more of sodium hyaluronate, niacinamide, red sage extract and astragalus extract.

8. The antioxidant astaxanthin emulsion of claim 7, wherein the skin conditioner consists of sodium hyaluronate, niacinamide, Salvia miltiorrhiza extract and Astragalus membranaceus extract mixed in a weight ratio of 1 (2.5-3.0): 1.5-2.0): 1.2-1.4.

9. A method of preparing an emulsion of antioxidant astaxanthin according to any one of claims 1 to 8, comprising the steps of:

s1: mixing xanthan gum with butanediol 57-65% of butanediol by weight, adding deionized water, glycerol, disodium EDTA, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, xanthan gum, glyceryl polyether-26 and methyl hydroxybenzoate, stirring, mixing, and keeping the temperature to obtain a mixture; mixing sodium hyaluronate with butanediol, adding Poria extract, nicotinamide, Saviae Miltiorrhizae radix extract and radix astragali extract, stirring, mixing, and mixing with the mixture to obtain water phase;

s2: stirring and mixing isostearyl alcohol, butanediol cocoate, ethyl cellulose, tocopherol acetate, cyclopentadimethyl siloxane, caprylic/capric triglyceride and C10-18 fatty acid triglyceride to obtain oil phase;

s3: stirring and mixing the water phase and the oil phase, adding triethanolamine, stirring and mixing, adding astaxanthin liposome, ethylhexylglycerin and bioglycan-1, stirring and mixing, filtering, and discharging.

10. The method for preparing an emulsion of astaxanthin according to claim 9, wherein the mesh number of the filtering is 300-400 mesh.

Technical Field

The application relates to the technical field of cosmetics, in particular to an antioxidant astaxanthin emulsion and a preparation method thereof.

Background

The skin is one of the largest organs of the human body and is the first natural barrier to protect the body from external aggressions. Over time, the skin undergoes endogenous and exogenous aging, endogenous aging being closely related to genetics, while exogenous aging is primarily influenced by the environment and the individual's lifestyle.

Research in the medical field shows that 80% of the aging phenomenon is caused by "free radicals" in the environment, and cells for maintaining skin balance are damaged and reduced due to the damage of the free radicals, and the immune capacity of skin cells is rapidly reduced, thereby causing skin relaxation.

Therefore, in order to delay aging and maintain skin elasticity, people generally care the skin with skin care skin having an antioxidant function. At present, most of antioxidant skin care products in the market are generally natural plant extracts, and although antioxidants from natural plants have high safety, the antioxidants have the problems of single antioxidant effect and poor antioxidant effect.

Disclosure of Invention

In order to improve the effect of resisting oxidation on skin and improve the skin elasticity, the application provides an antioxidant astaxanthin emulsion and a preparation method thereof.

In a first aspect, the application provides an antioxidant astaxanthin emulsion, which adopts the following technical scheme:

the antioxidant astaxanthin emulsion comprises the following components in percentage by weight:

0.45-0.55% of skin conditioner;

0.1 to 0.4 percent of antioxidant;

0.02-0.05% of chelating agent;

0.12-0.15% of preservative;

0.18 to 0.2 percent of pH regulator;

3-5% of glycerol;

2.0 to 3.0 percent of butanediol;

acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer 0.15-0.25%;

0.15-0.25% of xanthan gum;

261.5-2.5% of glyceryl polyether;

isostearyl alcohol 0.60-0.65%;

0.60 to 0.65 percent of butanediol cocoate;

0.60 to 0.65 percent of ethyl cellulose;

0.8 to 1.2 percent of cyclopentasiloxane;

caprylic/capric triglyceride 4.0-5.0%;

0.8-1.2% of C10-18 fatty acid triglyceride;

10.2 to 0.3 percent of biological carbohydrate gum;

0.45 to 0.50 percent of ethylhexyl glycerol;

0.02-0.05% of essence;

the balance of deionized water;

wherein the antioxidant is prepared by mixing astaxanthin, tocopherol acetate and Poria extract.

By adopting the technical scheme, the antioxidant mixed by the astaxanthin, the tocopherol acetate and the tuckahoe extract is added into the total raw material prepared by the antioxidant astaxanthin emulsion, and after a person uses the antioxidant astaxanthin emulsion, because of good synergistic effect of the astaxanthin, the tocopherol acetate and the tuckahoe extract, on one hand, the polyunsaturated fatty acid in the skin lipid membrane and the cell membrane of the horny layer can be prevented from being attacked by free radicals by supplying hydrogen ions to lipoxidation free radicals or lipodesc free radicals to interrupt lipid peroxidation chain reaction; on the other hand, the compound antioxidant forms carotenoid free radicals through addition reaction with free radicals, so that the content of the free radicals can be effectively reduced, and the antioxidant effect on the skin is improved; meanwhile, the astaxanthin, the tocopherol acetate and the tuckahoe extract which are compounded can also improve the content of hydroxyproline, inhibit the cross-linking of collagen molecules, promote the reproduction of fiber cells in the skin and repair aged and loose skin, thereby effectively improving the elasticity of the skin and improving the effect of resisting oxidation on the skin.

Preferably, the antioxidant astaxanthin emulsion consists of the following components in percentage by weight:

0.47-0.51% of skin conditioner;

0.16-0.28% of antioxidant;

chelating agent 0.026-0.038%;

0.13 to 0.14 percent of preservative;

0.18 to 0.19 percent of pH regulator;

3.4 to 4.2 percent of glycerin;

2.20 to 2.60 percent of butanediol;

acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer 0.17-0.21%;

0.17-0.21% of xanthan gum;

glyceryl polyether-261.70-2.21%;

isostearyl alcohol 0.61-0.63%;

0.61-0.63% of butanediol cocoate;

0.61-0.63% of ethyl cellulose;

0.88 to 1.04 percent of cyclopentadimethylsiloxane;

caprylic/capric triglyceride 4.20-4.60%;

c10-18 fatty acid triglyceride 0.888-1.04%;

10.22 to 0.26 percent of biological carbohydrate gum;

0.46 to 0.48 percent of ethylhexyl glycerol;

0.03-0.04% of essence;

the balance of deionized water;

wherein the antioxidant is prepared by mixing astaxanthin, tocopherol acetate and Poria extract;

the chelating agent is EDTA disodium;

the preservative is methyl hydroxybenzoate.

By adopting the technical scheme, the proportion of the raw materials is optimized, so that the finally prepared antioxidant astaxanthin emulsion has the relative variation of 21.05-23.21% in the skin elasticity of 3 weeks and 4.77-4.88% in the moisture content of the skin of 3 weeks when the volunteers use the emulsion for 3 weeks; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 40.35-44.64%, and the relative change of the moisture content of 6 weeks is 12.22-12.56%. Compared with the antioxidant astaxanthin emulsion prepared by non-optimized raw material proportion, the antioxidant astaxanthin emulsion prepared by optimized raw material proportion has better antioxidant effect on skin, and can obviously improve the elasticity of the skin and the water content of the skin.

Preferably, the antioxidant is prepared by mixing astaxanthin, tocopherol acetate and poria cocos extract according to the weight ratio of 1 (0.8-1.2) to 1.0-1.4.

By adopting the technical scheme, volunteers prepare the antioxidant emulsion by using the raw material ratio, and when the skin elasticity index of 3 weeks is 25.00-25.45% and the skin moisture content of 3 weeks is 5.16-5.25% in relative change at 3 weeks; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 48.21-50.91%, and the relative change of the moisture content of 6 weeks is 12.62-12.87%. Compared with the antioxidant astaxanthin emulsion prepared by the raw material ratio which is not optimized, the antioxidant astaxanthin emulsion prepared by optimizing the raw material ratio has better synergistic effect among the extraction of astaxanthin, tocopherol acetate and tuckahoe, can obviously improve the antioxidant effect on skin and improve the elasticity and the moisture content of the skin.

Preferably, the astaxanthin is astaxanthin liposome, and the preparation process of the astaxanthin liposome comprises the following steps: firstly stirring and mixing cholesterol, lecithin and ethanol, then adding astaxanthin, propylene glycol and deionized water, stirring and mixing, and then sequentially shearing and homogenizing to obtain the astaxanthin nano liposome.

By adopting the technical scheme, under the preparation method, the astaxanthin nanoliposome prepared from astaxanthin, cholesterol, lecithin and ethanol can wrap astaxanthin in the nanoliposome, so that on one hand, the astaxanthin in the nanoliposome can be slowly released, the degradation of the astaxanthin due to self-generation oxidation is reduced, the stability of the astaxanthin is improved, and the better synergistic effect of the astaxanthin, the tocopherol acetate and the poria extract is favorably realized; on the other hand, the formed astaxanthin nano liposome has the dual characteristics of hydrophilicity and lipophilicity, can effectively promote the absorption performance of the skin to the astaxanthin nano liposome, and is favorable for better exerting the antioxidation effect on the skin.

Preferably, the lecithin is any one of egg yolk lecithin and soybean lecithin.

By adopting the technical scheme, in the total raw materials for preparing the antioxidant astaxanthin emulsion, when a volunteer uses the antioxidant astaxanthin emulsion prepared from the egg yolk lecithin for 3 weeks, the relative change of the skin elasticity index of 3 weeks is 33.93%%, and the relative change of the moisture content of 3 weeks is 5.77%; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index at 6 weeks is 58.93%, and the relative change of the moisture content at 6 weeks is 13.65%. In the total raw materials for preparing the antioxidant astaxanthin emulsion, when a volunteer uses the antioxidant astaxanthin emulsion prepared by using egg yolk lecithin for 3 weeks, the relative change of the skin elasticity index of 3 weeks is 29.63 percent, and the relative change of the moisture content of 3 weeks is 5.38 percent; when the volunteer uses the composition for 6 weeks, the relative change of the skin elasticity index at 6 weeks is 55.56%, and the relative change of the moisture content at 6 weeks is 12.89%. Therefore, in the preparation process of the astaxanthin liposome, when the lecithin is egg yolk lecithin, the finally prepared antioxidant emulsion has better antioxidant effect, can obviously improve the antioxidant effect on the skin and improve the elasticity of the skin and the moisture content of the skin.

Preferably, in the preparation process of the astaxanthin liposome, the mixing temperature of the lecithin, the cholesterol and the ethanol is 45-55 ℃, and the shearing time is 15-25 min; the homogenizing pressure is 125-135 MPa.

By adopting the technical scheme, when the volunteers use the parameters in the range to prepare the antioxidant astaxanthin emulsion for 3 weeks, the relative change of the skin elasticity index of the emulsion for 3 weeks is 30.91-33.93 percent in the preparation process of the antioxidant astaxanthin emulsion; the relative change of the skin moisture content of 3 weeks is 5.52-5.77%; when the volunteers use the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 56.36-59.26%; the relative change of the moisture content of the skin at 6 weeks is 13.23-13.65%.

Preferably, the skin conditioner is one or more of sodium hyaluronate, nicotinamide, a red sage root extract and an astragalus extract.

By adopting the technical scheme, the sodium hyaluronate, the nicotinamide, the red sage root extract and the astragalus extract are added into the total raw materials for preparing the antioxidant astaxanthin emulsion, so that on one hand, the microcirculation of the skin can be promoted, the cell metabolism is improved, and the absorption and utilization of the antioxidant by the skin are facilitated; on the other hand, the salvia miltiorrhiza extract also contains active substances of salvianolic acid and salvianolic acid C, can enhance the content of hydroxyproline, has a synergistic interaction effect on antioxidants, can further inhibit the cross-linking of collagen molecules, and promotes the reproduction of fibroblasts in the skin; moreover, the skin conditioner can supplement skin moisture and deeply lock water, is beneficial to supplementing water and locking water for aged and loose skin when the aged and loose skin is repaired, and can effectively improve the skin elasticity.

Preferably, the skin conditioner is prepared by mixing sodium hyaluronate, nicotinamide, a red sage root extract and an astragalus extract according to the weight ratio of 1 (2.5-3.0) to (1.5-2.0) to (1.2-1.4).

By adopting the technical scheme, in the total raw materials for preparing the antioxidant astaxanthin emulsion, through optimizing the proportion, the sodium hyaluronate, the nicotinamide, the red sage root extract and the astragalus root extract have better synergistic effect, and the relative change of the elasticity index of the skin at 3 weeks of a volunteer is 42.86-46.30% and the relative change of the moisture content of the skin at 3 weeks of the antioxidant astaxanthin emulsion is 6.40-6.42%; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 67.86-72.22%, and the relative change of the moisture content of 6 weeks is 14.59-14.65%.

In a second aspect, the application provides a preparation method of an antioxidant astaxanthin emulsion, which adopts the following technical scheme: a preparation method of antioxidant astaxanthin emulsion comprises the following steps:

s1: mixing xanthan gum with butanediol 57-65% of butanediol by weight, adding deionized water, glycerol, disodium EDTA, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, xanthan gum, glyceryl polyether-26 and methyl hydroxybenzoate, stirring, mixing, and keeping the temperature to obtain a mixture; mixing sodium hyaluronate with butanediol, adding Poria extract, nicotinamide, Saviae Miltiorrhizae radix extract and radix astragali extract, stirring, mixing, and mixing with the mixture to obtain water phase;

s2: stirring and mixing isostearyl alcohol, butanediol cocoate, ethyl cellulose, tocopherol acetate, cyclopentadimethyl siloxane, caprylic/capric triglyceride and C10-18 fatty acid triglyceride to obtain oil phase;

s3: stirring and mixing the water phase and the oil phase, adding triethanolamine, stirring and mixing, adding astaxanthin liposome, ethylhexylglycerin and bioglycan-1, stirring and mixing, filtering, and discharging.

By adopting the technical scheme, the raw materials are mixed step by step, so that the dispersibility of the raw materials can be effectively improved, the texture of the finally prepared antioxidant astaxanthin emulsion is improved, the skin can be further helped to resist the absorption of the antioxidant astaxanthin emulsion, and a good antioxidant effect is achieved.

Preferably, the mesh number of the filtration is 300-400 meshes.

By adopting the technical scheme, after filtering, the finally prepared antioxidant astaxanthin emulsion is fine and uniform in texture, is convenient for skin to absorb the antioxidant astaxanthin emulsion, and further exerts good antioxidant effect.

In summary, the present application has the following beneficial effects:

1. due to the good synergistic effect of the astaxanthin, the tocopherol acetate and the poria cocos extract, the skin damage caused by free radicals is reduced and the propagation of fiber cells is promoted through the reaction with the free radicals, so that the antioxidant effect on the skin can be effectively improved, the aged skin is repaired, and the elasticity of the aged skin is improved;

2. the lecithin has the dual characteristics of hydrophilicity and lipophilicity, can effectively promote the skin to absorb the astaxanthin nano-liposome, is beneficial to slowly releasing the astaxanthin in the astaxanthin liposome, reduces the self-generation degradation of the astaxanthin, and improves the using effect of the astaxanthin;

3. according to the method, the raw materials are mixed step by step, so that the dispersibility of the raw materials can be effectively improved, the texture of the finally prepared antioxidant astaxanthin emulsion is improved, the skin can be further helped to absorb the antioxidant astaxanthin emulsion, and a good antioxidant effect is achieved.

Detailed Description

The present application will be described in further detail with reference to examples.

The raw materials used in the examples of the present application are commercially available except for the following specific descriptions.

Astaxanthin, purchased from the biotechnology limited of Wanming, Shanghai;

tocopheryl acetate, niacinamide, both purchased from imperial corporation;

poria extract, specification content 5:1, purchased from Sierran's Jersey Biotechnology Co., Ltd;

yolk lecithin with pH of 5.0-6.2, purchased from Guangzhou daily oil science and technology Co., Ltd;

soybean lecithin, CAS number 92128-87-5, available from Wuhan Povlov Biotech, Inc.;

cholesterol of the molecular formula C₂₇H_46OThe material is purchased from Yulongxiang biotechnology Limited Hebei;

sodium hyaluronate, purchased from forrestat, cosmetic grade;

the salvia miltiorrhiza extract, the water content of the product is less than 6%, is purchased from Shanxi Huidonda Biotech limited company;

radix astragali extract, cat # 10, purchased from Shanxi Hao Chen Biotech limited;

glyceryl polyether-26, hydroxyl value 128138mgKOH/g, purchased from Nantong Haishi chemical auxiliary agent Co., Ltd;

butanediol is obtained from Shanghai Aladdin Biotechnology GmbH, cat # B111018;

isostearyl alcohol, CAS number 41744-75-6;

ethyl cellulose having a CAS number of 9004-57-3;

cyclopenta dimethyl siloxane with CAS number 541-02-6, purchased from Jenpen silicon fluoride materials, Inc., Shenzhen;

caprylic/capric triglyceride, model GTCC, purchased from tsuzhou yuan tai run chemical ltd;

c10-18 triglycerides of fatty acids, purchased from Jiafa lion;

biogel-1, purchased from SOLABIA;

the shearing machine has the model of RHJ-039, and is purchased from Yu Xiang light industry machinery equipment Limited in Guangzhou city;

a high pressure homogenizer, model BH-0657, was purchased from Doubrio Bismith second-hand Equipment, Inc.

The test methods of examples and comparative examples of the present application are as follows

Skin elasticity test and skin moisture content test: selecting healthy volunteers between the ages of 25-30, continuously applying the antioxidant astaxanthin emulsion prepared in the embodiment of the application and the emulsion prepared in the comparative example to facial skin by 2mL each time after each person cleans the face every morning and evening, wherein the volunteers do not use other skin care products during the test period, and performing skin elasticity value detection and skin moisture content detection on the forehead skin of the volunteers at 8 am points of 0 week, third week and sixth week by using a skin elasticity instrument Cotomerma 580 of CK company, wherein the negative pressure of the skin elasticity instrument Cotomerma 580 is constantly 450mbar during the test. Among them, the closer the skin elasticity value is to 1, the better the skin elasticity is.

Preparation examples of raw materials

Preparation example 1

An astaxanthin liposome is prepared by the following steps: adding cholesterol, lecithin and ethanol into a stirrer at 45 ℃ and with the rotation speed set at 200r/min, stirring and mixing for 10min, adding astaxanthin, propylene glycol and deionized water into the stirrer, and stirring and mixing for 20min to obtain primary emulsion; adding the primary emulsion into a shearing machine, shearing for 15min under the condition that the rotating speed is set to 3000r/min to obtain a micron-sized astaxanthin dispersion, adding the micron-sized astaxanthin dispersion into a high-pressure homogenizer, and performing three times of circulating homogenization treatment under the conditions that the rotating speed is set to 3000r/min and 125MPa to obtain an astaxanthin nanoliposome;

wherein the lecithin is egg yolk lecithin.

Preparation example 2

An astaxanthin liposome which is different from that of preparation example 1 in that the weight ratio of cholesterol, lecithin, ethanol and astaxanthin is different, and the ratio of astaxanthin to cholesterol, lecithin, ethanol, propylene glycol and deionized water is shown in Table 1.

TABLE 1 proportions of astaxanthin to cholesterol, lecithin, ethanol, propylene glycol, deionized water in preparation examples 1-3

Preparation example 4

An astaxanthin liposome is different from the preparation example 2 in that the preparation step comprises mixing lecithin, cholesterol and ethanol at 50 deg.C, shearing for 20min, and homogenizing under 130 MPa.

Preparation example 5

An astaxanthin liposome is different from the preparation example 2 in that the preparation step comprises mixing lecithin, cholesterol and ethanol at 55 deg.C, shearing for 25min, and homogenizing under 135 MPa.

Preparation example 6

An astaxanthin liposome, which is different from the preparation example 4 in that the lecithin is soybean lecithin in the preparation step.

Examples

Example 1: the antioxidant astaxanthin emulsion comprises the components and the corresponding parts by weight shown in the table 1, and is prepared by the following steps:

s1: mixing xanthan gum and butanediol (in the embodiment, the preferred adding amount of butanediol is 61%) accounting for 57-65% of the weight of butanediol at 25 ℃ and the rotation speed of 200r/min for 10 min; adding deionized water, glycerol, disodium EDTA, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, xanthan gum, glycerol polyether-26 and methyl hydroxybenzoate under the conditions that the temperature is 75 ℃ and the rotating speed is set to be 800r/min, stirring and mixing for 30min, and keeping the temperature for 30min to obtain a mixture;

mixing sodium hyaluronate with the rest butanediol at 25 deg.C and rotation speed of 200r/min under stirring for 10min, adding Poria extract, nicotinamide, Saviae Miltiorrhizae radix extract and radix astragali extract, and mixing under stirring for 20 min; stirring and mixing the mixture for 20min under the conditions that the temperature is 75 ℃ and the rotating speed is set to be 700r/min to obtain a water phase;

s2: stirring and mixing isostearyl alcohol, butanediol cocoate, ethyl cellulose, tocopheryl acetate, cyclopentadimethyl siloxane, caprylic/capric triglyceride and C10-18 fatty acid triglyceride for 10min at 75 deg.C and rotation speed of 800r/min to obtain oil phase;

s3: pumping the oil phase into an emulsifying pot at 75 deg.C and rotation speed of 1200r/min, pumping the water phase into the emulsifying pot, and homogenizing for 10 min; adding triethanolamine at 60 deg.C and rotation speed of 1200r/min, stirring and mixing for 5 min; adding astaxanthin liposome, ethylhexyl glycerol and biological carbohydrate gum-1 at 45 deg.C and 1200r/min, stirring and mixing for 10min, filtering with 300 mesh filter cloth, and discharging.

Wherein the skin conditioner is sodium hyaluronate;

the antioxidant is prepared by mixing astaxanthin, tocopherol acetate and Poria extract at a weight ratio of 1:0.6: 0.8;

the chelating agent is EDTA disodium;

the preservative is methyl hydroxybenzoate;

the pH regulator is triethanolamine;

the essence is rose oil.

Examples 2 to 6

An antioxidant astaxanthin emulsion was different from example 1 in that each component and the corresponding weight thereof are shown in table 2.

TABLE 2 Components and weights (kg) thereof in examples 1-6

30 volunteers are selected and randomly divided into 6 groups, 5 volunteers are selected in each group, after each volunteer cleans the face in the morning and evening for 6 weeks, the antioxidant astaxanthin emulsion prepared in the application examples 1-6 is continuously applied to the facial skin, 2mL is applied each time, the volunteers do not use other skin care products in the test period, the skin elasticity value and the skin moisture content of the forehead skin of the volunteer are detected at 8 points in the morning at 0 week, the third week and the sixth week, the detection results are averaged and are counted in the following table 3.

Table 3 results of tests of examples 1 to 6

As can be seen from the data analysis in Table 3, the antioxidant astaxanthin emulsions prepared in examples 1-6 of the present application have a skin elasticity index of 0.66-0.69 and a relative change of 17.86-23.21% in 3 weeks when the emulsion is used by volunteers for 3 weeks; the moisture content of the skin is 44.00-47.15%, and the relative variation in 3 weeks is 4.42-4.88%; when the volunteer uses the composition for 6 weeks, the skin elasticity index is 0.76-0.81, and the relative variation is 35.17-44.64% in 6 weeks; the moisture content of the skin is 47.10-50.50%, and the relative variation in 6 weeks is 11.56-12.56%. Therefore, the antioxidant astaxanthin emulsion prepared by the method has a good antioxidant effect on skin, and can improve the elasticity of the skin and the water content of the skin.

In particular, when the antioxidant astaxanthin emulsion prepared in the example 2-4 is used by volunteers for 3 weeks, the relative variation of the skin elasticity is 21.05-23.21% in 3 weeks, and the relative variation of the moisture content of the skin is 4.77-4.88% in 3 weeks; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 40.35-44.64%, and the relative change of the moisture content of 6 weeks is 12.22-12.56%. Therefore, in the preparation process of the total raw materials of the antioxidant astaxanthin emulsion, the antioxidant astaxanthin emulsion prepared by the raw materials with the content in the range of the examples 2-4 has better antioxidant effect on the skin, and can obviously improve the elasticity of the skin and the water content of the skin. In particular, the antioxidant astaxanthin emulsion obtained in example 3 was more effective in improving skin elasticity and skin moisture content than in examples 2 and 4.

Comparative examples 1 to 7

An emulsion differing from example 3 in the content and composition of astaxanthin, tocopheryl acetate and poria cocos extract, the astaxanthin, tocopheryl acetate and poria cocos extract and their respective weights are shown in table 4.

TABLE 4 comparative examples 1-7 for the components and their weights (kg)

Selecting 35 volunteers, randomly dividing the volunteers into 5 groups, randomly cleaning the face of each group of 5 volunteers in the morning and evening, continuously applying the antioxidant astaxanthin emulsion prepared in comparative examples 1-7 of the application for 6 weeks to the facial skin, applying the antioxidant astaxanthin emulsion for 2mL each time, not using other skin care products during the test period, performing skin elasticity value detection and skin moisture content detection on the forehead skin of the volunteers at 8 points in the morning of 0 week, third week and sixth week, averaging the detection results and counting the average values into the following list 5.

TABLE 5 test results of comparative examples 1 to 7

As can be seen from the data analysis in tables 3 and 5, the volunteers using the emulsion of antioxidant astaxanthin obtained in example 3 of the present application showed a relative change in the skin elasticity index at 3 weeks of 23.21% and a relative change in the moisture content at 3 weeks of 4.77%; when the volunteer uses the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 44.64 percent; the relative change of the skin moisture content at 6 weeks was 12.27%.

The comparative example 1 and example 3 differ in that the astaxanthin content is 0. When the volunteer used the emulsion prepared in comparative example 1 for 3 weeks, the relative change of the skin elasticity index at 3 weeks was 7.69%; the relative change of the skin moisture content at 3 weeks is 2.32%; when the volunteer uses the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 13.85 percent; the relative change of the skin moisture content at 6 weeks was 4.80%. According to the variation of the performances of the emulsion prepared by the volunteer in example 3 and the emulsion prepared by the volunteer in comparative example 1, the performances of the emulsion prepared by the volunteer in comparative example 1 are lower than those of the emulsion prepared by the volunteer in example 3. It is thus shown that, in the preparation of the total raw materials of the antioxidant astaxanthin emulsion of the present application, the addition of no astaxanthin reduces the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion, resulting in poor effects of improving skin elasticity and skin moisture content.

Comparative example 2 differs from example 3 in that the tocopherol acetate content is 0. When the volunteer used the emulsion prepared in comparative example 1 for 3 weeks, the relative change of the skin elasticity index was 9.09% at 3 weeks; the relative change of the skin moisture content at 3 weeks is 2.19%; when the volunteers use the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 15.15 percent; the relative change of the skin moisture content at 6 weeks was 4.71%. According to the variation of the performances of the emulsion prepared by the volunteer in example 3 and the emulsion prepared by the volunteer in comparative example 2, the performances of the emulsion prepared by the volunteer in comparative example 2 are lower than those of the emulsion prepared by the volunteer in example 3. It is thus shown that, in the preparation of the total raw material of the antioxidant astaxanthin emulsion of the present application, the addition of tocopherol acetate does not lower the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion, resulting in poor effects of improving skin elasticity and skin moisture content.

The difference between comparative example 3 and example 3 is that the content of ester of poria extract is 0. When the volunteer used the emulsion prepared in comparative example 3 for 3 weeks, the relative change of the skin elasticity index at 3 weeks was 9.23%; the relative change of the skin moisture content at 3 weeks is 2.17%; when the volunteers use the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 15.38 percent; the relative change of the skin moisture content at 6 weeks was 4.69%. According to the variation of the performances of the emulsion prepared by the volunteer in example 3 and the emulsion prepared by the volunteer in comparative example 3, the performances of the emulsion prepared by the volunteer in comparative example 3 are lower than those of the emulsion prepared by the volunteer in example 3. It is thus shown that, in the preparation of the total raw materials of the antioxidant astaxanthin emulsion of the present application, the addition of the poria cocos extract does not lower the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion, resulting in poor effects of improving skin elasticity and skin moisture content.

Comparative example 4 differs from example 3 in that the content of astaxanthin and tocopheryl acetate is 0. When the volunteer used the emulsion prepared in comparative example 4 for 3 weeks, the relative change of the skin elasticity index at 3 weeks was 4.55%; the relative change of the skin moisture content at 3 weeks is 1.21%; when the volunteers use the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 7.58 percent; the relative change of the skin moisture content at 6 weeks was 3.55%. According to the variation of the performances of the emulsion prepared by the volunteer using the example 3 and the emulsion prepared by the volunteer using the comparative example 4, the performances of the emulsion prepared by the volunteer using the comparative example 4 are lower than those of the emulsion prepared by the volunteer using the example 3. It is thus shown that, in the preparation of the total raw materials of the antioxidant astaxanthin emulsion of the present application, the addition of no astaxanthin and no tocopherol acetate reduces the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion, resulting in poor effects of improving skin elasticity and skin moisture content.

The comparative example 5 and example 3 are different in that the contents of astaxanthin and poria cocos wolf extract are 0. When the volunteer used the emulsion prepared in comparative example 5 for 3 weeks, the relative change of the skin elasticity index was 3.08% for 3 weeks; the relative change of the skin moisture content at 3 weeks is 1.12%; when the volunteer uses the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 7.69 percent; the relative change of the skin moisture content at 6 weeks was 3.38%. According to the variation of the performances of the emulsion prepared by the volunteer using the example 3 and the emulsion prepared by the volunteer using the comparative example 5, the performances of the emulsion prepared by the volunteer using the comparative example 5 are lower than those of the emulsion prepared by the volunteer using the example 3. It is thus shown that, in the preparation of the total raw materials of the antioxidant astaxanthin emulsion of the present application, the addition of astaxanthin and poria cocos extract does not lower the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion, resulting in poor effects of improving skin elasticity and skin moisture content.

The comparative example 6 is different from the example 3 in that the contents of tocopherol acetate and tuckahoe extract are 0. When the volunteer used the emulsion prepared in comparative example 6 for 3 weeks, the relative change of the skin elasticity index at 3 weeks was 3.03%; the relative change of the skin moisture content at 3 weeks is 1.14%; when the volunteer uses the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 9.09%; the relative change of the skin moisture content at 6 weeks was 3.42%. According to the variation of the performances of the emulsion prepared by the volunteer in example 3 and the emulsion prepared by the volunteer in comparative example 6, the performances of the emulsion prepared by the volunteer in comparative example 6 are lower than those of the emulsion prepared by the volunteer in example 3. It is thus shown that, in the preparation of the total raw materials of the antioxidant astaxanthin emulsion of the present application, the addition of tocopherol acetate and poria cocos extract does not lower the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion, resulting in poor effects of improving skin elasticity and skin moisture content.

Comparative example 7 is different from example 3 in that the contents of astaxanthin, tocopherol acetate and poria cocos wolf extract are 0. When the volunteer used the emulsion prepared in comparative example 7 for 3 weeks, the relative change of the skin elasticity index at 3 weeks was 1.54%; the relative change of the skin moisture content at 3 weeks is 0.44%; when the volunteer uses the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 3.08 percent; the relative change of the skin moisture content at 6 weeks was 2.32%. According to the variation of the performances of the emulsion prepared by the volunteer in example 3 and the emulsion prepared by the volunteer in comparative example 7, the performances of the emulsion prepared by the volunteer in comparative example 7 are lower than those of the emulsion prepared by the volunteer in example 3. It is thus shown that, in the preparation of the total raw materials of the antioxidant astaxanthin emulsion of the present application, the absence of astaxanthin, tocopherol acetate and poria extract reduces the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion, resulting in poor effects of improving skin elasticity and skin moisture content.

In conclusion, in the preparation process of the total raw materials of the antioxidant astaxanthin emulsion, the astaxanthin, the tocopherol acetate and the poria cocos extract have good synergistic effect, and the antioxidant effect of the antioxidant astaxanthin emulsion on skin can be obviously improved, so that the skin elasticity and the skin water content are better improved.

Examples 7 to 10

An antioxidant astaxanthin emulsion was prepared in the same manner as in example 3 except that the antioxidant was used in a different weight ratio of astaxanthin, tocopherol acetate and poria cocos wolf extract, and the amounts of astaxanthin, tocopherol acetate and poria cocos wolf extract and their respective weights are shown in Table 6.

TABLE 6 astaxanthin, tocopherol acetate and Poria extract and their weights (kg) in examples 7-10

20 volunteers were selected and randomly divided into 5 groups, 5 persons in each group were selected, after each volunteer cleaned their face in the morning and evening, the antioxidant astaxanthin emulsion prepared in examples 7-10 of the present application was continuously applied to the facial skin for 6 weeks, 2mL each time, no other skin care product was used during the test period, skin elasticity value and skin moisture content were measured on the forehead skin of the volunteer at 8 am at 0 week, third week and sixth week, and the measurement results were averaged and counted in the following table 7.

TABLE 7 test results of examples 7 to 10

As can be seen from the data analysis in Table 7, the relative change of the skin elasticity index at 3 weeks was 25.00-25.45% in the case of 3 weeks in which the antioxidant astaxanthin emulsion prepared in examples 7-9 of the present application was used by the volunteers; the relative change of the skin moisture content of 3 weeks is 5.16-5.25%; when the volunteer uses the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 48.21-50.91%; the relative change of the moisture content of the skin at 6 weeks is 12.62-12.87%. According to the variation of the properties of the antioxidant astaxanthin emulsion obtained by the volunteers using the examples 7 to 9 and the antioxidant astaxanthin emulsion obtained by the volunteers using the example 3, the properties of the antioxidant astaxanthin emulsion obtained by the volunteers using the examples 7 to 9 are higher than those of the antioxidant astaxanthin emulsion obtained by the volunteers using the example 3. Therefore, the antioxidant is prepared by mixing astaxanthin, tocopherol acetate and poria cocos extract according to the weight ratio of 1 (0.8-1.2) to 1.0-1.4, the synergistic effect among astaxanthin, tocopherol acetate and poria cocos extract is the best, the antioxidant effect on the skin can be obviously improved, and the elasticity and the moisture content of the skin are improved.

Examples 11 to 16

An antioxidant astaxanthin emulsion was prepared in the same manner as in example 8 except that astaxanthin was produced in the astaxanthin liposomes prepared in preparation examples 1, 2, 3, 4, 5 and 6 in this order as shown in Table 8, except that astaxanthin was used in the following manner, and the examples 11 to 16 were prepared as shown in Table 8.

TABLE 8 corresponding preparations in examples 11 to 15

30 volunteers were selected and randomly divided into 6 groups, 5 individuals in each group, after each volunteer cleaned their face in the morning and evening, the antioxidant astaxanthin emulsion prepared in examples 11 to 16 of the present application was continuously applied to the facial skin for 6 weeks, 2mL each time, no other skin care product was used during the test period, skin elasticity and skin moisture content were measured on the forehead skin of the volunteer at 8 am at 0 week, third week and sixth week, and the measurement results were averaged and recorded in the following table 9.

TABLE 9 results of tests in examples 11 to 16

As can be seen from the data analysis in Table 9, the relative change of the skin elasticity index at 3 weeks was 31.48-33.33%%, when the volunteers used the antioxidant astaxanthin emulsion prepared in examples 11-13 of the present application for 3 weeks; the relative change of the skin moisture content of 3 weeks is 5.44-5.55%; when the volunteers use the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 57.41-59.26%; the relative change of the moisture content of the skin at 6 weeks is 12.98-13.32%.

According to the variation of the properties of the emulsion of antioxidant astaxanthin obtained by the volunteers using the emulsion of examples 11-13 and the emulsion of antioxidant astaxanthin obtained by the volunteers using the emulsion of example 8, the properties of the emulsion of example 11-13 are higher than those of the emulsion of antioxidant astaxanthin obtained by the volunteers using the emulsion of example 8. Therefore, the astaxanthin is prepared into the astaxanthin nanoliposome in the total raw materials for preparing the antioxidant astaxanthin emulsion, the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion is better, and particularly when the astaxanthin, cholesterol, lecithin, ethanol, propylene glycol and deionized water are mixed according to the weight ratio of 1:0.55:2.0:3.0, the antioxidant effect on the skin can be obviously improved, and the elasticity of the skin and the moisture content of the skin are improved.

When the volunteers used the antioxidant astaxanthin emulsion prepared in the application examples 14 to 15 for 3 weeks, the relative change of the skin elasticity index of the emulsion for 3 weeks is 30.91 to 33.93 percent; the relative change of the skin moisture content of 3 weeks is 5.52-5.77%; when the volunteers use the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 56.36-59.26%; the relative change of the moisture content of the skin at 6 weeks is 13.23-13.65%.

According to the variation of the properties of the emulsion of antioxidant astaxanthin obtained by the volunteers using the emulsion of examples 14-15 and the emulsion of antioxidant astaxanthin obtained by the volunteers using the emulsion of example 8, the properties of the emulsion of example 14-15 are higher than those of the emulsion of example 8. Therefore, in the preparation process of the astaxanthin liposome, when the mixing temperature of lecithin, cholesterol and ethanol is 45-55 ℃, the shearing time is 15-25min, and the homogenizing pressure is 125-135MPa, the finally prepared antioxidant astaxanthin emulsion has better antioxidant effect, can obviously improve the antioxidant effect on the skin, and improve the elasticity of the skin and the moisture content of the skin.

When the volunteers used the emulsion of antioxidant astaxanthin prepared in example 16 of the present application for 3 weeks, the relative change of the skin elasticity index at 3 weeks was 29.63%; the relative change of the skin moisture content at 3 weeks is 5.38%; when the volunteer uses the skin elasticity index for 6 weeks, the relative change of the skin elasticity index for 6 weeks is 55.56 percent; the relative change of the skin moisture content at 6 weeks was 12.89%.

According to the variation of the performances of the emulsion of antioxidant astaxanthin obtained by the volunteers using the emulsion of example 16 and the emulsion of antioxidant astaxanthin obtained by the volunteers using the emulsion of example 8, the performances of the emulsion of example 16 are higher than those of the emulsion of example 14. Therefore, in the preparation process of the astaxanthin liposome, when the lecithin is egg yolk lecithin, the finally prepared antioxidant astaxanthin emulsion has better antioxidant effect, can obviously improve the antioxidant effect on the skin, and improves the elasticity of the skin and the moisture content of the skin.

In conclusion, especially the antioxidant astaxanthin emulsion prepared in the example 14 of the present application has better performances than the emulsion prepared in the examples 11, 12, 13, 15 and 16.

Examples 17 to 23

An antioxidant astaxanthin emulsion was prepared as in example 12 except that the skin conditioner contained sodium hyaluronate, nicotinamide, the extract of Salvia miltiorrhiza Bunge and the extract of Astragalus membranaceus Bunge in different amounts, and the amounts and weights of the sodium hyaluronate, nicotinamide, the extract of Salvia miltiorrhiza Bunge and the extract of Astragalus membranaceus Bunge were as shown in Table 10.

TABLE 10 compositions and weights (kg) of skin conditioners of examples 17-23

35 volunteers were selected and randomly divided into 7 groups, 5 individuals in each group, after each volunteer cleaned their face in the morning and evening for 6 weeks, the antioxidant astaxanthin emulsion prepared in examples 17 to 23 of the present application was applied to the facial skin 2mL each time, no other skin care product was used during the test period, skin elasticity and skin moisture content were measured on the forehead skin of the volunteer at 8 am at 0 week, third week and sixth week, and the measurement results were averaged and included in the following table 11.

TABLE 11 test results of examples 17 to 23

As can be seen from the data analysis in Table 11, when the volunteers used the emulsions of examples 17 to 18 of the present application to prepare the antioxidant astaxanthin emulsion for 3 weeks, the relative change of the skin elasticity index for 3 weeks was 35.7 to 36.36%, and the relative change of the skin moisture content for 3 weeks was 5.81 to 5.95%; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 60.71-61.82%, and the relative change of the moisture content of 6 weeks is 13.72-14.05%.

According to the variation of the properties of the antioxidant astaxanthin emulsion obtained by the volunteers using the emulsions obtained in examples 17 to 18 and the antioxidant astaxanthin emulsion obtained by the volunteers using the emulsion obtained in example 8, the properties of the emulsions obtained by the volunteers using the emulsions obtained in examples 11 to 13 are higher than those of the emulsions obtained by the volunteers using the emulsion obtained in example 8.

When the volunteers use the antioxidant astaxanthin emulsion prepared by the application examples 20-22 for 3 weeks, the relative change of the skin elasticity index of 3 weeks is 42.86-46.30%, and the relative change of the moisture content of 3 weeks is 6.40-6.42%; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 67.86-72.22%, and the relative change of the moisture content of 6 weeks is 14.59-14.65%.

When the volunteers use the antioxidant astaxanthin emulsion prepared in the application examples 19 and 23 for 3 weeks, the relative change of the skin elasticity index of 3 weeks is 39.29-40.00%, and the relative change of the skin moisture content of 3 weeks is 6.07%; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 64.29-65.45%, and the relative change of the moisture content of 6 weeks is 13.93-14.02%.

The variation of the properties of the emulsions of astaxanthin obtained from the volunteers according to examples 17 to 22 and the emulsions of astaxanthin obtained from the volunteers according to example 14 can be shown, and the properties of the emulsions of examples 17 to 22 are higher than those of the emulsions of example 14. Particularly, in the preparation process of the total raw materials of the antioxidant astaxanthin emulsion, when the skin conditioner is prepared by mixing sodium hyaluronate, nicotinamide, a red sage root extract and an astragalus root extract according to the weight ratio of 1 (2.5-3.0) to (1.5-2.0) to (1.2-1.4), the antioxidant astaxanthin emulsion finally prepared has better antioxidant effect, can obviously improve the antioxidant effect on the skin, and improve the elasticity of the skin and the moisture content of the skin. In particular, the antioxidant astaxanthin emulsion prepared in example 21 of the present application has better performances than those of examples 20 and 22.

Examples 24 to 25

An antioxidant astaxanthin emulsion was prepared in the same manner as in example 21 except that the mixing temperature of the mixture was changed in the preparation of the antioxidant astaxanthin emulsion, and the mixing temperature of the mixture was as shown in Table 12.

TABLE 12 mixing temperatures of the mixtures of examples 24-25

10 volunteers were selected and randomly divided into 2 groups, 5 individuals in each group, after each volunteer cleaned their face in the morning and evening for 6 weeks, the antioxidant astaxanthin emulsion prepared according to examples 24 to 25 of the present application was applied to the facial skin 2mL each time, no other skin care product was used during the test period, skin elasticity and skin moisture content were measured on the forehead skin of the volunteer at 8 am at 0 week, third week and sixth week, and the measurement results were averaged and included in the following table 11.

TABLE 13 test results of examples 24 to 25

From the data analysis in table 13, it can be seen that, when the volunteers used the emulsions of the antioxidant astaxanthin prepared in examples 24 and 25 of the present application for 3 weeks, the relative change of the skin elasticity index at 3 weeks was 48.15-45.45%, and the relative change of the moisture content at 3 weeks was 6.51-6.62%; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 72.73-75.93%, and the relative change of the moisture content of 6 weeks is 15.12-15.24%.

According to the variation of the performance of the emulsion of antioxidant astaxanthin obtained by the volunteers in examples 24 and 25 and the emulsion of antioxidant astaxanthin obtained by the volunteers in example 21, the performance of the emulsion of the volunteers in examples 26 and 27 is higher than that of the emulsion of antioxidant astaxanthin obtained by the volunteers in example 21. Therefore, in the preparation process of the antioxidant astaxanthin emulsion, when the mixing temperature of the mixture is 77.5-80 ℃, the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion is better, the antioxidant effect on skin can be improved, and the elasticity of the skin and the moisture content of the skin can be improved.

In particular, the antioxidant astaxanthin emulsion obtained in example 24 of the present application exhibited better properties than those of example 25.

Examples 26 to 27

An antioxidant astaxanthin emulsion was prepared in the same manner as in example 24 except that the mesh number of the filter cloth was changed in the preparation of the antioxidant astaxanthin emulsion, and the mesh number of the filter cloth of the mixture was as shown in Table 14.

TABLE 14 mesh number of filter cloths in examples 26 to 27

10 volunteers were selected and randomly divided into 2 groups, 5 individuals in each group, after each volunteer cleaned their face in the morning and evening, the antioxidant astaxanthin emulsion prepared in examples 26 to 27 of the present application was continuously applied to the facial skin for 6 weeks, 2mL each time, no other skin care product was used during the test period, skin elasticity and skin moisture content were measured on the forehead skin of the volunteer at 8 am at 0 week, third week and sixth week, and the measurement results were averaged and included in the following table 15.

TABLE 15 test results of examples 26 to 27

From the data analysis in table 15, it can be seen that, when the volunteers used the emulsions of the present application, examples 26 and 27, prepared the antioxidant astaxanthin emulsion for 3 weeks, the relative change of the skin elasticity index for 3 weeks was 48.15-50.00%, and the relative change of the moisture content of 3 weeks was 6.53-6.59%; when the volunteer uses the skin for 6 weeks, the relative change of the skin elasticity index of 6 weeks is 75.93-77.78%, and the relative change of the moisture content of 6 weeks is 15.29-15.35%.

According to the variation of the performances of the emulsion of antioxidant astaxanthin obtained by the volunteers using the examples 26 and 27 of the present application and the emulsion of antioxidant astaxanthin obtained by the volunteers using the example 24 of the present application, the performances of the emulsion of the volunteers using the examples 26 and 27 of the present application are higher than those of the emulsion of antioxidant astaxanthin obtained by the volunteers using the example 24 of the present application. Therefore, in the preparation process of the antioxidant astaxanthin emulsion, when the filtering mesh number is 300-400 meshes, the antioxidant effect of the finally prepared antioxidant astaxanthin emulsion is better, the antioxidant effect on skin can be obviously improved, and the elasticity of the skin and the moisture content of the skin are improved.

In particular, the antioxidant astaxanthin emulsion prepared in example 26 of the present application has better performances than those of example 27.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.