阅读说明：本技术 一种促进蚯蚓生长的添加剂 (Additive for promoting growth of earthworms ) 是由 伏春燕 孙涛 朱应波 李霞 董以雷 刘雪兰 魏祥法 管延同 于 2021-09-26 设计创作，主要内容包括：本发明公开了一种促进蚯蚓生长的添加剂。本发明从促进蚯蚓肠道有益微生物增殖、提高蚯蚓消化吸收及对蚯蚓进行诱食的角度出发,采用黄腐酸、异麦芽低聚糖、菊粉、甜味素、山楂粉为原料复配制成促进蚯蚓生长的添加剂,经实验证明,其具有显著的促进蚯蚓生长的功能。(The invention discloses an additive for promoting the growth of earthworms. The additive for promoting the growth of the earthworms is prepared by compounding fulvic acid, isomaltooligosaccharide, inulin, aspartame and hawthorn powder serving as raw materials from the viewpoints of promoting the proliferation of beneficial microorganisms in the intestinal tracts of the earthworms, improving the digestion and absorption of the earthworms and attracting the earthworms, and experiments prove that the additive has a remarkable function of promoting the growth of the earthworms.)

1. An additive for promoting the growth of earthworms is characterized by being prepared by compounding fulvic acid, isomaltooligosaccharides, inulin, a phagostimulant and a water-soluble carrier, wherein the fulvic acid content in the additive is 5-10%, the isomaltooligosaccharides content is 5-15%, and the inulin content is 20-30%.

2. The additive for promoting the growth of earthworms as claimed in claim 1, wherein the phagostimulant is a sweetener and hawthorn powder.

3. The additive for promoting the growth of the earthworms as claimed in claim 2, which is prepared from the following raw materials in percentage by weight: 10-15% of fulvic acid with the content of more than or equal to 50%, 10-20% of isomaltooligosaccharide with the content of more than or equal to 50%, 25-30% of inulin, 5-10% of sweetener, 5-10% of hawthorn powder and the balance of water-soluble carrier to 100%.

4. The additive for promoting the growth of earthworms according to claim 3, wherein the water-soluble carrier is starch.

5. The additive for promoting the growth of the earthworms as claimed in claim 4, wherein the additive comprises the following raw materials in percentage by weight: 12-13% of fulvic acid with the content of more than or equal to 50%, 15-20% of isomaltooligosaccharide with the content of more than or equal to 50%, 28-30% of inulin, 8-9.5% of sweetener, 7-9% of hawthorn powder and the balance of starch to 100%.

6. Use of the additive of any one of claims 1-5 for promoting growth of earthworms.

7. The method for using the additive as claimed in any one of claims 1 to 5, wherein the additive is prepared into 4000-5000ppm aqueous solution, and the aqueous solution is periodically sprayed on the earthworm cultivation substrate to keep the substrate humidity at 50 +/-5%.

Technical Field

The invention belongs to the field of animal nutrition, relates to an additive, and particularly relates to an additive for promoting earthworm growth.

Background

Earthworms, also known as earthworms, are used as valuable pharmaceuticals for treating various diseases, and also as high-protein foods and feeds, and more importantly, earthworms can treat domestic garbage, organic wastes, purify sewage, and the like. The earthworms are common land omnivorous animals, and leaves, straws, livestock and poultry manure, domestic garbage, activated sludge, leftovers of paper making and food industries and the like can be eaten, so the earthworms can eliminate environmental pollution to a certain extent, and earthworm cultivation plants are established in many countries at present. Earthworms eat large amounts, which correspond to their own weight per day, so that the efficiency of treating organic waste with earthworms is very high, and it is reported that 40 tons of organic waste can be swallowed by 1 hundred million earthworms in one day.

In recent years, intensive large-scale breeding is rapidly developed and gradually becomes the main body of the development of animal husbandry in China, but small farms (households) still account for a large proportion, and due to the shortage of funds, the lack of technologies and equipment for treating the excrement and the great lack of the technologies and equipment for treating the excrement, the small farms (households) mostly do not meet the environmental protection requirement, and the survival and the development of the small farms (households) are seriously influenced, so that a new idea for treating the excrement and the sewage needs to be explored. In view of the strong organic matter digestion capacity and the wide feeding property of the earthworms, the method for breeding the earthworms by using the excrement of the farm is a better idea for treating the excrement of the livestock and the poultry.

The method for breeding earthworms by using livestock and poultry manure and crude fiber raw materials (such as crop straws, herb residues and the like) to prepare a matrix is the most common method for breeding earthworms at present, for example, 201510947686.X discloses a method for breeding earthworms by using cow manure and straw fermentation as a matrix, and CN201810702582.6 discloses a method for breeding earthworms by using chicken manure, straw powder and other raw materials to ferment as a matrix. CN202010033633.8 discloses a method for breeding earthworms by using herb residues of epimedium powder and cow dung as substrates. Because the carbon-nitrogen ratio of the livestock and poultry manure is low (the carbon-nitrogen ratio of chicken manure and pig manure is less than 20, and the carbon-nitrogen ratio of cow manure is less than 25), and the suitable carbon-nitrogen ratio of earthworm food is about 30, a certain proportion of crude fiber raw materials are added into the livestock and poultry manure, on one hand, the carbon-nitrogen ratio of the base material is improved, and on the other hand, the odor of the livestock and poultry manure is reduced. However, according to earthworm breeding experiments, the feed intake and growth of earthworms are adversely affected due to the increase of the content of crude fibers, and therefore, an additive for promoting the growth of earthworms is developed.

Disclosure of Invention

Aiming at the problems, the invention provides an additive for promoting the growth of earthworms. The additive for promoting the growth of the earthworms is prepared by compounding fulvic acid, isomaltooligosaccharide, inulin, aspartame and hawthorn powder serving as raw materials from the viewpoints of promoting the proliferation of beneficial microorganisms in the intestinal tracts of the earthworms, improving the digestion and absorption of the earthworms and attracting the earthworms, and experiments prove that the additive has a remarkable function of promoting the growth of the earthworms.

The technical scheme of the invention is as follows: an additive for promoting the growth of earthworms is characterized by being prepared by compounding fulvic acid, Isomaltooligosaccharides (IMO), inulin, a phagostimulant and a water-soluble carrier, wherein the fulvic acid content of the additive is 5-10%, the isomaltooligosaccharides content is 5-15%, and the inulin content is 20-30%.

Wherein, the feeding promoting agent is preferably sweetener and hawthorn powder, and the water-soluble carrier is preferably starch.

Preferably, the additive comprises the following raw materials in percentage by weight: 10-15% of fulvic acid (the content is more than or equal to 50%), 10-20% of isomaltooligosaccharide (the content is more than or equal to 50%), 25-30% of inulin, 5-10% of sweetener, 5-10% of hawthorn powder and 100% of water-soluble carrier.

More preferably, the weight ratio of the raw materials of the additive is as follows: 12-13% of fulvic acid (the content is more than or equal to 50%), 15-20% of isomaltooligosaccharide (the content is more than or equal to 50%), 28-30% of inulin, 8-9.5% of sweetener, 7-9% of hawthorn powder and the balance of starch (water-soluble carrier) to 100%.

The using method comprises the following steps: preparing 4000-.

The function of each component in the invention is as follows:

fulvic acid (biochemical fulvic acid): the growth promoter contains multiple active enzymes, can improve the digestion and metabolism of animals, promote the growth of animals and improve the production performance of animals; the contained nucleic acid has the biological functions of improving the cell life activity and repairing cell biomembranes; the contained macronutrients can improve the permeability of animal cell membranes and promote the absorption of nutrients by the macronutrients.

Isomaltooligosaccharides: the isomaltooligosaccharide is absorbed and decomposed by beneficial bacteria in animal intestinal tract, inhibits exogenous pathogenic bacteria and proliferation of intrinsic putrefying bacteria in intestinal tract, and reduces production of toxic fermentation product and harmful bacteria. The mechanism is that the beneficial bacteria and the epithelial cells of the intestinal mucosa can be matched with each other to occupy the surface of the intestinal mucosa, a biomembrane barrier with a protection effect is formed, invasion of harmful flora is prevented, proliferation of the harmful flora in the intestinal tract is inhibited, the intestinal environment is improved, and the intestinal tract is protected.

Inulin: the rhizome of jerusalem artichoke (jerusalem artichoke) is used as a raw material, protein, colloid, crude fiber and mineral substances are removed, and the jerusalem artichoke is obtained by the production processes of water extraction, ion exchange, membrane filtration, spray drying and the like. Inulin is a natural water-soluble fiber, and is utilized by beneficial microorganisms in the intestinal tract, so that the intestinal environment is improved; can enhance the peristalsis of the digestive tract, improve digestion and increase appetite; short-chain fatty acids generated by fermentation of inulin in intestinal tract can lower colon pH, inhibit growth of saprophytic bacteria, and reduce generation of toxic products.

Aspartame (aspartame): is synthesized by condensing L-aspartic acid and L-phenylalanine methyl ester hydrochloride, has strong sweet taste, and is a good phagostimulant for earthworms.

Hawthorn powder: is prepared by grinding hawthorn, and the contained organic acids such as tartaric acid, citric acid, crataegolic acid, malic acid and the like are good phagostimulants for earthworms; the contained lipolytic enzyme can promote digestion of food, thereby having food calling and digestion promoting functions.

Starch: is prepared by accurately mixing water-soluble starch, various inorganic salts and special auxiliary materials, has the characteristics of stability, easy digestion and absorption, low sweetness and instant dissolution, and is a good carrier.

The invention has the beneficial effects that: the fulvic acid, the isomaltooligosaccharide, the inulin, the sweetener and the hawthorn powder are compounded, and the components are synergistic, so that a plurality of active enzymes contained in the fulvic acid can improve the digestion and metabolism of the earthworms and the growth of the earthworms; the isomaltooligosaccharide can inhibit the proliferation of exogenous pathogenic bacteria and intrinsic putrefying bacteria in the intestines of the earthworms, reduce the generation of toxic fermentation products and harmful bacteria, and maintain the health of the intestines; the inulin can enhance the peristalsis of the earthworm digestive tract, improve digestion and increase appetite; the sweetener has strong sweet taste and has the effect of inducing earthworms to eat; the fructus crataegi powder has effects of promoting food intake and promoting digestion. Therefore, the invention promotes the growth of the earthworms from multiple angles, such as induction of earthworm feeding, improvement of earthworm digestion and absorption functions, improvement of earthworm intestinal health and the like. The test proves that: the additive can promote the growth of earthworms and maintain the balance of intestinal flora of the earthworms.

Detailed Description

The invention will be further illustrated with reference to the following examples, wherein fulvic acid is provided by the company Oppson Fertilizer, Inc. of south-Guangxi, has a fulvic acid content of not less than 50%, a water solubility of not less than 98%, and a pH value: 5-7. Isomaltooligosaccharides (isomaltooligosaccharides-500) were produced by Shandong bowling Biotechnology Ltd, and the content was 50%. Inulin is produced by Shandong New sugar resource agriculture science and technology Limited company, and the content is more than or equal to 92 percent. The sweetener is produced by Baiyou Biotech limited, Hebei. The hawthorn powder is produced by Tianjin Xiugu biotechnology development limited. Starch is produced by Weifang Boyuan chemical Limited.

Example 1

The proportioning (weight ratio) is as follows: 11.5 percent of fulvic acid, 17 percent of isomaltooligosaccharide, 27.5 percent of inulin, 7 percent of sweetener, 7 percent of hawthorn powder and 30 percent of starch.

The using method comprises the following steps: directly dissolved in water, and the concentration is 4000-5000 ppm.

Example 2

The proportioning (weight ratio) is as follows: 12.5 percent of fulvic acid, 18 percent of isomaltooligosaccharide, 28.5 percent of inulin, 7 percent of sweetener, 8 percent of hawthorn powder and 26 percent of starch.

The using method comprises the following steps: directly dissolved in water, and the concentration is 4000-5000 ppm.

Example 3

The proportioning (weight ratio) is as follows: 15% of fulvic acid, 20% of isomaltooligosaccharide, 30% of inulin, 10% of sweetener, 10% of hawthorn powder and 15% of starch.

The using method comprises the following steps: directly dissolved in water, and the concentration is 3000-4000 ppm.

Experimental example 1

The invention adopts a foam box to breed earthworms in a laboratory for a bench test in 3 months in 2020. The matrix is as follows: mixing the fermented chicken manure and the Chinese medicine residues, and adjusting the carbon-nitrogen ratio to be 30.

And (3) experimental design: the test is totally provided with 4 groups, 6 are set in each group, 20 young earthworms are repeatedly stocked and the average initial weight is recorded, the humidity of the substrate is regulated and controlled by the first group, the second group and the third group by using the additive aqueous solution prepared by the formula of the embodiment 1, the concentration is 4000ppm, 4500ppm and 5000ppm respectively, and the humidity of the substrate is regulated and controlled by the fourth group by using drinking water.

The test method comprises the following steps: the culture substrate components are completely the same, the substrate humidity and temperature of each group are consistent (the temperature is 23 ℃, and the humidity is 50%) in the test period, and the earthworms of each group are weighed and the average body weight gain is calculated after 4 weeks of the test. The test results are shown in Table 1.

TABLE 1 bench test results

Group of	Group 1	2 groups of	Group 3	4 groups of
					Weight gain (mg/bar)	0.154±0.031ab	0.159±0.021ab	0.177±0.017a	0.094±0.003b

As can be seen from table 1, the weight gain of the earthworms of groups 1, 2 and 3 is significantly larger than that of group 4. The results of the small tests show that the invention has obvious growth promoting effect, and the growth promoting effect has no obvious difference within the concentration range of 4000-5000 ppm.

Experimental example 2

On the basis of the above small tests, a breeding comparison pilot test was carried out in the ecological breeding base of the cattle village, town and blue sea in east Ying district, Dongying City, 4 months in 2020, and a comparison was carried out according to whether the feed additive (the formula of example 1) of the present invention was added or not. Two rows of fresh earthworm beds with the same matrix are selected (the matrix is the same as the matrix), the humidity of the earthworm bed is adjusted by using the additive with the concentration of 5000ppm in the 1 st row, the humidity of the earthworm bed is adjusted by using drinking water in the 2 nd row, the earthworm stocking amount in the two rows is the same, the feeding and management mode is the same (the temperature is 23 ℃, the humidity is 50%), and the test period is 6 months. The comparative experimental results are shown in table 2.

Table 2 pilot test results

	Column 1	Column 2
			Stocking amount (kg)	20	20
Weight of harvested earthworm (kg)	53.35	39.50

As can be seen from Table 2, the weight of earthworms harvested by the technology of the invention is 13.85 kg more than that of the control, so that the effect of promoting the growth of the earthworms is very obvious, and the weight gain rate is 35.1%.