阅读说明：本技术 一种朱红硫磺菌及其应用 (Sulfur red mushroom and application thereof ) 是由 孙长春 于 2021-03-17 设计创作，主要内容包括：本发明涉及微生物技术领域,尤其是涉及一种朱红硫磺菌及其应用。本发明的菌株具有与SEQ ID NO:1所示的至少99％一致的18S rDNA-ITS序列。本发明的菌株可以显著降低白细胞介素6(IL-6)和肿瘤坏死因子(TNF-α)的水平,进而治疗和预防白细胞介素6(IL-6)和/或肿瘤坏死因子(TNF-α)通路异常相关疾病,其起效快,绿色、安全、可靠。(The invention relates to the technical field of microorganisms, and particularly relates to sulfur cinnabar and application thereof. The strain of the invention has an 18S rDNA-ITS sequence at least 99% identical to that shown in SEQ ID NO. 1. The strain can obviously reduce the levels of interleukin 6(IL-6) and tumor necrosis factor (TNF-alpha), so as to treat and prevent diseases related to the abnormal pathway of the interleukin 6(IL-6) and/or the tumor necrosis factor (TNF-alpha), and the strain has the advantages of quick response, greenness, safety and reliability.)

1. A strain having an 18S rDNA-ITS sequence at least 99% identical to that set forth in SEQ ID NO. 1.

2. The strain of claim 1, which is Laetiporus cinnabarinus (Laetiporus Sulphureus) deposited at the China general microbiological culture Collection center (CGMCC) at 12/3 of 2020 with the collection number of CGMCC No. 21084.

3. Use of the strain of claim 1 or 2 for the preparation of a medicament for the treatment or prevention of an interleukin-6 pathway abnormality disease.

4. The use of claim 3, wherein the disease comprises autoimmune disease, tumor and metabolic disease.

5. Use of a strain according to claim 1 or 2 for the preparation of a medicament for the treatment or prevention of disorders in the TNF- α pathway.

6. The use of claim 5, wherein the disease comprises one or more of psoriasis, inflammatory bowel disease, rheumatoid arthritis, ankylosing spondylitis, dry eye disease, acute anterior uveitis, severe sepsis.

7. A pharmaceutical composition comprising the strain of claim 1 or 2 and a pharmaceutically acceptable carrier.

8. The pharmaceutical composition of claim 7, wherein the pharmaceutical composition is a tablet, capsule, oral dosage form, or lyophilized powder.

9. The pharmaceutical composition of claim 7, wherein the pharmaceutically acceptable carrier comprises one or more of skim milk, lactose, dextrose, sucrose, sorbitol, mannose, trehalose, starch, gum arabic, calcium phosphate, alginates, gelatin, calcium silicate, fine crystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, mineral oil.

10. A food or health product for the treatment or co-treatment of inflammation, autoimmune diseases and tumours, comprising a strain according to claim 1 or 2.

Technical Field

The invention relates to the technical field of microorganisms, and particularly relates to sulfur cinnabar and application thereof.

Background

Laetiporus cinnabarinus, Chinese alias Laetiporus sulphureus variant, Thielavia rubrum, cockscomb and the like, belong to Basidiomycota (Basidiomycota), Agaricaceae (Agaricamyces), Aphyllophorales (Polyporales), Laetiporus (Laetiporus), are widely distributed in China's Heilongjiang, Hebei, Sichuan, Tibet, Xinjiang, Taiwan and other areas, and are large-scale fungi for both rare and edible medicines. The fruiting body is edible when tender to maturity, has delicious taste and rich nutrition, is cheese-shaped after aging, is not suitable for eating, but can be used as a medicine. The traditional Chinese medicine is warm in nature and sweet in taste, has various effects of improving the immunity of a human body and the like, but has unclear treatment indications in modern diseases due to an unclear specific mechanism.

Interleukin-6 (IL-6) is a multifunctional cytokine produced by stromal cells, epithelial cells and other cells, and plays an important role in the processes of proliferation and differentiation of B lymphocytes, T lymphocytes and hematopoietic stem cells, inflammatory reactions, autoimmune diseases, growth of certain tumor cells and the like. IL-6 is a double-edged sword. The classical signal path is only expressed in the liver and the immune system, and plays a role in inhibiting inflammation; the trans-signaling pathway is present in almost all cells and plays a role in promoting inflammation. Serum IL-6 levels have been widely used clinically as a marker of inflammation. The elevated level of IL-6 is closely related to the severity of inflammatory responses, the active phase of autoimmune diseases, tumor progression, and organ transplant rejection. Elevation of IL-6 is associated with a variety of diseases, such as (1) autoimmune diseases, such as Rheumatoid Arthritis (RA), Costlemon's disease, psoriasis, and the like; (2) tumors such as Multiple Myeloma (MM), plasmacytoma, chronic obstructive pulmonary disease, cervical cancer, hodgkin's disease, and the like; (3) burns, post-operative, acute infections, organ transplant rejection, etc. (4) Metabolic diseases: such as diabetes and atherosclerosis. IL-6 antibodies blocking IL-6 signaling pathways have become a focus of research and development, such as IL-6 antibody Tolizumab, and the indications of the IL-6 antibody are mainly autoimmune diseases such as rheumatoid arthritis and juvenile idiopathic arthritis. Other IL-6 antibody biologics already or pending are indicated for diseases: giant Cell Arteritis (GCA), Takayasu Arteritis (TA), nodular arteritis, and nodular arteritis, systemic lupus erythematosus, glomerulonephritis, multiple myeloma, refractory scleritis, central nervous system leukemia positive for CD19, acute B lymphocyte leukemia positive for CD19, and ulcerative colitis.

Tumor necrosis factor alpha (TNF-alpha) is also a potent cytokine, which is mainly produced by macrophages and is involved in many physiological and pathological processes of the human body, including autoimmune, cancer suppression, and inflammation. TNF-alpha is also a double-edged sword with one handle, on one hand, it participates in the physiological processes of hematopoiesis, innate immunity, bacterial infection protection and the like which are beneficial to human health; on the other hand, its abnormal regulation may also cause pathological processes such as rheumatoid arthritis, myocardial necrosis and tumor formation. The level of the anti-TNF-alpha antibody is also considered to be related to a plurality of diseases, several main TNF-alpha antibodies on the market at present comprise biological drugs such as adalimumab (adalimumab) hurmet, infliximab (infliximab) and the like, and of the 25 best-sold drugs in 2014 and 2015, 3 anti-TNF-alpha monoclonal antibodies in the first 5. The current approved or pending indications are: psoriasis, inflammatory bowel disease Crohn's disease and ulcerative colitis, rheumatoid arthritis, ankylosing spondylitis, dry eye disease, acute anterior uveitis, severe sepsis.

Currently, patients receiving IL-6 or TNF- α antibody therapy are at an increased risk of developing infection, liver dysfunction, and dyslipidemia, which can lead to hospitalization or death of the patient.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the sulfur cinnabar and the application thereof, the sulfur cinnabar can obviously reduce the level of interleukin 6(IL-6) and/or tumor necrosis factor (TNF-alpha), treat and prevent related diseases of abnormal pathways of the interleukin 6(IL-6) and/or the tumor necrosis factor (TNF-alpha), and has the advantages of quick response, greenness, safety and reliability, and good economic benefit and social benefit.

In order to achieve the purpose, the invention adopts the technical scheme that:

in a first aspect, the invention provides a strain having an 18S rDNA-ITS sequence at least 99% identical to that set forth in SEQ ID NO. 1.

As a preferred embodiment of the strain, the strain is Laetiporus cinnabarinus (Laetiporus Sulphur) ttys-997, and the Laetiporus cinnabarinus (Laetiporus Sulphur) ttys-997 is preserved in China general microbiological culture Collection center (CGMCC) at 12-3.2020 with the preservation addresses as follows: no. 3 Xilu No. 1 Beijing, Chaoyang, and the preservation number is CGMCC No. 21084.

The invention discloses a strain capable of reducing the levels of interleukin 6(IL-6) and tumor necrosis factor (TNF-alpha), the strain has an 18S rDNA-ITS sequence which is at least 99% identical to that shown in SEQ ID NO. 1, the length of the sequence is 578bp, and the strain is identified as Laetiporus (Laetiporus) and is Laetiporus sulpholeurus (Laetiporus cinnabarinus) through gene comparison. The classification unit is Eukaryota; fungi; dikarya; basidiosmyleta; agaricomyctina; agaricomyces; polyporales; laetiporus.

The Laetiporus cinnabarinus ttys-997 can obviously reduce the levels of inflammatory response indexes interleukin 6(IL-6) and tumor necrosis factor (TNF-alpha), so that diseases related to the abnormal pathways of the interleukin 6(IL-6) and the tumor necrosis factor (TNF-alpha) can be treated and prevented.

The Laetiporus cinnabarinus ttys-997 grows vigorously on a solid culture medium, hyphae are dense, the culture is carried out at 25-28 ℃, the colony is round, the surface is dry, and a large amount of aerial hyphae are generated after 5 days, and yellow pigment is generated.

The second aspect of the invention provides the application of the strain in preparing medicines for treating or preventing interleukin-6 pathway abnormal diseases.

As a preferred embodiment of the use according to the invention, the diseases include autoimmune diseases, tumors and metabolic diseases. Preferably, the autoimmune disease comprises psoriasis, rheumatoid arthritis, juvenile idiopathic arthritis, giant cell arteritis, Takayasu arteritis, nodular arteritis, macrovasculitis such as nodular arteritis, systemic lupus erythematosus, glomerulonephritis, multiple myeloma, refractory scleritis, neuromyelitis optica, atopic dermatitis, allergic rhinitis, celiac disease, asthma, inflammatory bowel disease, ankylosing spondylitis, Behcet's disease. Tumors include multiple myeloma, plasmacytoma, chronic obstructive pneumonia, cervical cancer, lung cancer, breast cancer, liver cancer, lymphoma, CD 19-positive central nervous system leukemia, and CD 19-positive acute B-lymphocyte leukemia. The metabolic diseases include diabetic macular edema, diabetic peripheral nerve injury, and atherosclerosis. The strain of the invention can also treat diseases such as burn, postoperative, acute infection, organ transplant rejection and the like, such as Cytokine Release Syndrome (CRS).

The third aspect of the invention provides the application of the strain in preparing a medicament for treating or preventing the TNF-alpha channel abnormal diseases.

As a preferred embodiment of the use according to the invention, the disease comprises one or more of psoriasis, inflammatory bowel disease, rheumatoid arthritis, ankylosing spondylitis, dry eye disease, acute anterior uveitis, severe sepsis.

The fourth aspect of the invention provides a pharmaceutical composition, which comprises the strain and a pharmaceutically acceptable carrier.

As a preferred embodiment of the pharmaceutical composition of the present invention, the pharmaceutical composition is a tablet, a capsule, an oral preparation or a lyophilized powder.

As a preferred embodiment of the pharmaceutical composition of the present invention, the pharmaceutically acceptable carrier includes one or more of skim milk, lactose, glucose, sucrose, sorbitol, mannose, trehalose, starch, gum arabic, calcium phosphate, alginate, gelatin, calcium silicate, fine crystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, mineral oil.

The fifth aspect of the invention provides a food or health care product for treating or assisting in treating inflammation, autoimmune diseases and tumors, which is characterized by containing the strain.

More preferably, the food or health product comprises any one of the above-mentioned strain, a lysate containing the above-mentioned strain, a culture supernatant containing the above-mentioned strain, and a metabolite containing the above-mentioned strain.

Compared with the prior art, the invention has the following beneficial effects:

the invention provides sulfur vermilion (Laetiporus sufureus) ttys-997, which can reduce the level of inflammatory response indexes IL-6 and TNF-alpha, is in a dose effect relationship, can treat IL-6 and TNF-alpha pathway abnormality related diseases, and has the advantages of quick response, greenness, safety and reliability.

Drawings

FIG. 1 is a diagram showing the result of gene alignment of 18S rDNA-ITS sequence amplified by Laetiporus cinnabarinus (Laetiporus Sulphureus) ttys-997.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to the accompanying drawings and specific embodiments.

Example 1 deposit information of Thiobacillus cinnabarinus (Laetiporus Sulphureus) ttys-997

The sulfur cinnabarinus (Laetiporus Sulphureus) ttys-997 of the invention is preserved in the China general microbiological culture Collection center in 12-3.2020, with the preservation addresses as follows: no. 3 Xilu No. 1 Beijing, Chaoyang, and the preservation number is CGMCC No. 21084.

Example 2 isolation, identification and culture of Thiobacillus cinnabarinus ttys-997

Collecting fruiting body of strain from stump, collecting fruiting body, aseptically processing, placing on PDA plate, standing at 28 deg.C, and culturing for 14 days to obtain filamentous mycelium. A part of filamentous mycelium was put into liquid medium and shake-cultured for 28 days with shaking, and a large amount of red granules were produced. The red granules are centrifugally collected and subjected to genetic analysis and identification, the strain has an 18S rDNA-ITS fragment (shown as SEQ ID NO: 1), the length of the sequence is 578bp, and the strain is identified as Laetiporus (Laetiporus) which is Laetiporus sulfoureus (Laetiporus) by combining with the appearance morphology of a sporophore. The classification unit is Eukaryota; fungi; dikarya; basidiosmyleta; agaricomyctina; agaricomyces; polyporales; laetiporus. The invention is named as Laetiporus sulphide (Laetiporus sulphureus) ttys-997.

Laetiporus cinnabarinus ttys-997 grows vigorously on a conventional solid culture medium on the market, hyphae are dense, the culture is carried out at 25-28 ℃, the colony is round, the surface is dry, and a large amount of aerial hyphae are generated after 5 days, and yellow pigment is generated.

Example 3 Effect of the Strain on the serum IL-6 and TNF-alpha content in mice model of inflammation

1. Animal model: rats are used as animal models, and a colitis model is constructed by intragastric administration of 5% dextran sodium sulfate.

2. Experimental animals: SD rat, male 180-220 g. (feeding environment: 22 +/-2 ℃, humidity of 45-65%, 12h day and night alternation); can be taken freely and drunk freely.

3. The experimental method comprises the following steps:

1) molding: the animals were fed in a single cage, and except for the blank group, the other 5 groups were continuously gavaged with 5% dextran sulfate sodium (0.75g/kg) for 10 days, 1 time per day, and the blank group was given an equal amount of physiological saline.

2) Grouping and administration:

rats were randomly divided into a blank group, a model group, experimental low, medium and high dose groups (1 mL/100g, 2mL/100g and 4mL/100g of Laetiporus Sulphureus) ttys-997 and intestinal tract regulation 0.15g/kg, 10 rats each. The administration is carried out by intragastric administration after 8 hours from the intragastric administration of dextran sodium sulfate on day 1 for 14 days.

3) Material taking:

the day before material drawing, raising in a single cage, observing the shape of the excrement, and measuring and scoring by using an excrement occult blood kit. Rats were fasted for 12 hours after the last dose, anesthetized with ether, and bled from the inferior vena cava for interleukin 6(IL-6), tumor necrosis factor (TNF- α) assays.

4) Detecting the index

Serum IL-6 and TNF-alpha levels were observed.

TABLE 1 Effect of the strains of the invention on rat serum IL-6 and TNF-. alpha.levels (mean. + -. SD)

Group of	Number of animals	IL-6(pg/mL)	TNF-α(pg/mL)
				Control group	10	21.86±9.73	28.23±15.23
Model set	10	68.72±24.09**	57.25±21.83**
				Group of 0.15g/kg for intestine-regulating	10	39.98±16.69##	30.89±9.55##
Low dose group	10	57.68±30.14	45.09±17.95
				Middle dose group	10	43.26±17.31##	36.31±13.82##
High dose group	10	36.53±16.01##	30.11±11.47##

^**P is less than 0.01 compared with a control group;^#P＜0.05，^##p is less than 0.01 compared with the model group;

as shown in Table 1, IL-6 and TNF-alpha in the model group were significantly increased compared to the control group; compared with the model group, the group with 0.15g/kg intestinal regeneration and the group with 0.15g/kg intestinal regeneration both significantly reduce the IL-6 and the TNF-alpha (P is less than 0.01), and the IL-6 and the TNF-alpha in the experimental dose group and the high dose group both significantly reduce (P is less than 0.05; P is less than 0.01) and are in dose effect relationship. The results show that the sulfur vermilion (Laetiporus sukusurus) ttys-997 has better effect of reducing the level of serum IL-6 and TNF-alpha of rat chronic colitis caused by sodium dextran sulfate, and the sulfur vermilion (Laetiporus sukusurus) ttys-997 can treat diseases (including inflammatory diseases, autoimmune diseases and tumors) related to IL-6 and TNF-alpha channel abnormality. The strain has quick response, is green, safe and reliable, and has good economic and social benefits when being used for treating diseases related to IL-6 and TNF-alpha pathway abnormality.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

SEQUENCE LISTING

<110> Sun Changchun

<120> Laetiporus cinnabarinus and application thereof

<130> 2021.01.26

<160> 1

<170> PatentIn version 3.5

<210> 1

<211> 578

<212> DNA

<213> Artificial Synthesis

<400> 1

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aagtaatgtg aattgcagaa ttcagtgaat cattgaatct ttgaacgcac cttgcgctcc 300

ttggcattcc gaggagcatg cctgtttgag tgtcatgaaa ctctcaaccc ctgccatctt 360

tgcggatgag cgtcggttgg attttggagg ttgccggact cgttcggctc ctcttgaaag 420

catagtaaag cttggacctg accgaccggg tggacgtgat agaaagtcac cgtcgaccga 480

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