阅读说明：本技术 一种应用于水生无脊椎动物毒性试验的流水测试系统 (Running water test system applied to toxicity test of aquatic invertebrates ) 是由 苏来 张乐 于 2020-10-16 设计创作，主要内容包括：本发明公开了一种应用于水生无脊椎动物毒性试验的流水测试系统,包括测试系统试验设计4个供试品浓度和一个空白对照,先配制4个供试品储备液和稀释水,然后通过蠕动泵将供试品储备液按比例用稀释水稀释成暴露液,混合均匀后,再通过蠕动泵将暴露液按照相同的流量输送至暴露单元中,采用溶液分流装置将暴露液分流至试验容器中,暴露系统采用水浴进行控温,试验容器设有溢流口,溢流口处覆有相配合的筛网。本发明集成了稀释单元和暴露单元,为水生无脊椎动物或其它小型的水生动物提供了便利的暴露系统,减轻了手动更换暴露液的工作量,流水系统有利于一些如挥发性、水解等难测试物质在暴露液中浓度的维持。(The invention discloses a flowing water test system applied to toxicity tests of aquatic invertebrates, which comprises a test system for testing 4 concentrations of test articles and a blank control, wherein 4 stock solutions of the test articles and dilution water are prepared firstly, then the stock solutions of the test articles are diluted into exposure liquid by the dilution water in proportion through a peristaltic pump, after uniform mixing, the exposure liquid is conveyed into an exposure unit according to the same flow through the peristaltic pump, a solution shunting device is adopted to shunt the exposure liquid into a test container, the temperature of the exposure system is controlled by a water bath, the test container is provided with an overflow port, and the overflow port is covered with a matched screen. The invention integrates the dilution unit and the exposure unit, provides a convenient exposure system for aquatic invertebrates or other small aquatic animals, reduces the workload of manually replacing the exposure liquid, and the flowing water system is favorable for maintaining the concentration of substances which are difficult to test, such as volatility, hydrolysis and the like, in the exposure liquid.)

1. A flowing water test system applied to aquatic invertebrate toxicity tests comprises a dilution unit and an exposure unit, and is characterized in that:

the test system experiment designs 4 sample concentrations and a blank control, 4 sample stock solutions and dilution water are prepared firstly, then the sample stock solutions are diluted into exposure liquid by the dilution water in proportion through a peristaltic pump, after the mixture is uniform, the exposure liquid is conveyed to an exposure unit through the peristaltic pump according to the same flow, the exposure liquid is shunted to a test container through a solution shunting device, the temperature of the exposure system is controlled by a water bath, the test container is provided with an overflow port, the overflow port is covered with a matched screen for preventing a sample organism from separating from the test container, and waste liquid generated by each container is collected by a waste liquid collecting system and then is concentrated and discharged.

2. The flow test system for toxicity testing in aquatic invertebrates of claim 1, wherein the stock solutions are designated as S1, S2, S3 and S4 and the dilution water is designated as DW.

3. The flow testing system for toxicity testing in aquatic invertebrates of claim 1, wherein the peristaltic pump setting is labeled PP 1.

4. The flow test system for toxicity testing in aquatic invertebrates of claim 1, wherein the system comprises a flow meter for measuring flow rate of the fluidThe exposure liquid is set at A, B, C and D, and the blank exposure liquid is set at C₀。

5. The flow testing system for toxicity testing in aquatic invertebrates of claim 1, wherein a peristaltic pump is provided and designated as PP 2.

6. The flow test system of claim 1, wherein the test containers are labeled as A1, A2, A3, A4, B1, B2, B3, B4, C1, C2, C3, C4, C4, and C1₀₁、C₀₂、C₀₃、C₀₄D1, D2, D3 and D4.

7. The running water test system for toxicity test of aquatic invertebrates according to claim 1, wherein the overflow port is provided with a cover with air holes for blocking dust and toxic materials from falling and performing air exchange through the air holes to provide oxygen for daphnia magna.

Technical Field

The invention relates to the field of test systems, in particular to a flowing water test system applied to toxicity tests of aquatic invertebrates.

Background

Invertebrates (Invertebrate) are animals without a spine on the dorsal side, which are the original form of the animal. The number of species accounts for 95% of the total number of species of animals. There are about 100 or more than ten thousand species present around the world. Including protozoa, echinoderms, mollusks, platyhelminths, annelids, coelenterates, arthropods, wireforms, and the like.

A branch of zoology. In animal classification, animals are classified into two major classes, vertebrate and invertebrate, according to the presence or absence of vertebrae in the animal body. The science of studying invertebrate classification, morphology, physiological characteristics, geographic distribution, reproduction, evolution, and the like, is called invertebrate science. Invertebrate biology includes protozoa, helminths, entomology, molluscs, crustaceans, and the like.

In a flow test system for performing invertebrate toxicity testing, the prior methods suffer from the following drawbacks:

in the existing test system, the exposure liquid needs to be manually replaced, so that the working environment is easily damaged when the test system works, and meanwhile, the workload is greatly increased.

Meanwhile, the concentration of some substances difficult to test, such as volatility, hydrolysis and the like, in the flowing water system in the exposure liquid is difficult to maintain, and the test result can be deviated.

Disclosure of Invention

The present invention is directed to a flow test system for toxicity testing of aquatic invertebrates, which solves the above problems.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a be applied to aquatic invertebrate toxicity test's flowing water test system, including 4 sample concentrations of test system experimental design and a blank control, prepare 4 sample reserves and dilution water earlier, then dilute the sample reserve into exposure liquid with the dilution water in proportion through the peristaltic pump, after the misce bene, the rethread peristaltic pump will expose the liquid and carry to exposing in the unit according to the same flow, adopt solution diverging device to shunt exposure liquid to experimental container, exposure system adopts the water bath to control the temperature, experimental container is equipped with the overflow mouth, the overflow mouth covers there is the matched with screen cloth, a discharge system in the concentration after preventing the biological separation of experiment container that supplies to test, the waste liquid that each container produced passes through waste liquid collecting system and collects.

Preferably, the stock solutions are set as labeled S1, S2, S3 and S4 and the dilution water is labeled DW.

Preferably, the peristaltic pump arrangement is labeled PP 1.

Preference is given toThe exposure liquid setting marks are A, B, C and D, and the blank control exposure liquid is set to C₀。

Preferably, a peristaltic pump is provided, designated PP 2.

Preferably, the test containers are provided with the designations A1, A2, A3, A4, B1, B2, B3, B4, C1, C2, C3, C4, C2₀₁、C₀₂、C₀₃、C₀₄D1, D2, D3 and D4.

Preferably, the overflow port is provided with a cover with air holes for blocking dust and toxic substances from falling, and performing gas exchange through the air holes to provide oxygen required by daphnia magna.

The invention has the technical effects and advantages that:

(1) the dilution unit and the exposure unit are integrated, a convenient exposure system is provided for aquatic invertebrates or other small aquatic animals, and the workload of manually replacing the exposure liquid is reduced.

(2) The flow system is beneficial to maintaining the concentration of some difficult-to-test substances such as volatility, hydrolysis and the like in the exposure liquid.

(3) The exposure unit adopts water bath temperature control, and integrates a waste liquid collection system and a solution shunting device, thereby providing convenience for test exposure.

(4) The test container is targeted and designed carefully, and provides a good exposure environment for raising and exposing the aquatic invertebrates and small-sized aquatic animals so as to obtain better test data.

Drawings

FIG. 1 is a schematic diagram of the system of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a flowing water test system applied to toxicity tests of aquatic invertebrates, which is shown in figure 1 and comprises 4 test article concentrations and a blank control, wherein 4 test article stock solutions and dilution water are firstly prepared, then the test article stock solutions are diluted into exposure liquid by the dilution water in proportion through a peristaltic pump, after the uniform mixing, the exposure liquid is conveyed to an exposure unit through the peristaltic pump according to the same flow, a solution shunting device is adopted to shunt the exposure liquid to a test container, the exposure system adopts water bath for temperature control, the test container is provided with an overflow port, a matched screen is covered at the overflow port and used for preventing a test organism from separating from the test container, and waste liquid generated by each container is collected by a waste liquid collection system and then is centrally discharged out of the system;

stock solutions were set as labeled S1, S2, S3 and S4, dilution water as DW, peristaltic pump as PP1, exposure liquid as A, B, C and D, and blank control exposure liquid as C₀The peristaltic pump is set as PP2, and the test container is set as A1, A2, A3, A4, B1, B2, B3, B4, C1, C2, C3, C4₀₁、C₀₂、C₀₃、C₀₄D1, D2, D3 and D4, the overflow port is provided with a cover with air holes for blocking dust and toxic substances from falling in and performing gas exchange through the air holes to provide oxygen required by daphnia magna, the dilution unit and the exposure unit are integrated, a convenient exposure system is provided for the aquatic invertebrates or other small-sized aquatic animals, and the workload of manually replacing the exposure liquid is reduced.

The flow system is beneficial to maintaining the concentration of some difficult-to-test substances such as volatility, hydrolysis and the like in the exposure liquid.

The exposure unit adopts water bath temperature control, and integrates a waste liquid collection system and a solution shunting device, thereby providing convenience for test exposure.

The test container is targeted and designed carefully, and provides a good exposure environment for raising and exposing the aquatic invertebrates and small-sized aquatic animals so as to obtain better test data.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.