阅读说明：本技术 一种实现物料混匀的微流控装置及混匀控制方法 (Micro-fluidic device for realizing material mixing and mixing control method ) 是由 林宝宝 邹瑜亮 李保 杨毅 于 2021-10-07 设计创作，主要内容包括：本发明提供一种实现物料混匀的微流控装置及混匀控制方法,包括微流控芯片本体以及分别用于容纳不同物料的至少两个容纳瓶体,微流控芯片本体内构造有微流控管道,至少两个容纳瓶体分别处于设于微流控芯片本体上的瓶套内,且两个容纳瓶体分别对应设置有具有针口的针体,两个容纳瓶体通过分别具有的针体与微流控管道能够形成连通,容纳瓶体能够在轴向力的作用下沿瓶套的轴向直线运动以使针体由第一密封状态转换为流通状态,第二密封组件能够在轴向力的作用下靠近第一密封组件以使物料释放。本发明,容纳瓶体与微流控芯片集成于一体,两个容纳瓶体内的物料能够在彼此之间流动实现物料的均匀混合,进而保证检测结果的准确性。(The invention provides a microfluidic device for realizing material mixing and a mixing control method, which comprise a microfluidic chip body and at least two containing bottle bodies respectively used for containing different materials, wherein a microfluidic pipeline is constructed in the microfluidic chip body, the at least two containing bottle bodies are respectively positioned in a bottle sleeve arranged on the microfluidic chip body, the two containing bottle bodies are respectively and correspondingly provided with a needle body with a needle opening, the two containing bottle bodies can be communicated with the microfluidic pipeline through the needle bodies respectively, the containing bottle bodies can linearly move along the axial direction of the bottle sleeve under the action of axial force so as to enable the needle bodies to be converted into a circulation state from a first sealing state, and a second sealing component can be close to the first sealing component under the action of the axial force so as to enable the materials to be released. According to the invention, the containing bottle bodies and the microfluidic chip are integrated, and materials in the two containing bottle bodies can flow between each other to realize uniform mixing of the materials, so that the accuracy of a detection result is ensured.)

1. The micro-fluidic device for realizing material mixing is characterized by comprising a micro-fluidic chip body (1) and at least two containing bottle bodies (2) which are used for containing different materials respectively, a micro-fluidic pipeline (11) is constructed in the micro-fluidic chip body (1), the at least two containing bottle bodies (2) are arranged in bottle sleeves (3) arranged on the micro-fluidic chip body (1) respectively, needle bodies (4) with needle openings (41) are correspondingly arranged on the two containing bottle bodies (2) respectively, the two containing bottle bodies (2) can be communicated with the micro-fluidic pipeline (11) through the needle bodies (4) which are arranged respectively, a first sealing component and a second sealing component are connected with a first end and a second end in the axial direction of each containing bottle body (2) respectively to form an inner sealed containing space for containing the bottle bodies (2), the needle body (4) is provided with a first sealing state that the needle opening (41) is positioned in the first sealing assembly, the needle opening (41) is positioned in a circulating state in the inner sealing accommodating space, the accommodating bottle body (2) can move linearly along the axial direction of the bottle sleeve (3) under the action of axial force so that the needle body (4) is converted into the circulating state from the first sealing state, and the second sealing assembly can be close to the first sealing assembly under the action of the axial force so that the materials are released.

2. Microfluidic device according to claim 1, characterized in that the needle body (4) further has a second sealing condition of the needle mouth (41) within the second sealing assembly, which is also capable of switching the needle body (4) from the flow-through condition to the second sealing condition under the action of the axial force.

3. The microfluidic device according to claim 2, wherein the first sealing assembly comprises a first rubber plug (51), a protective cover (52), and the needle opening (41) is in the first rubber plug (51) when the needle body (4) is in the first sealing state; the second sealing component comprises a second rubber plug (61) and a hard gasket (62) arranged on one side, deviating from the first rubber plug (51), of the second rubber plug (61), and the needle body (4) is in the second sealing state, and the needle opening (41) is arranged in the second rubber plug (61).

4. The microfluidic device according to claim 3, wherein the first end is a throat structure, the first rubber plug (51) is a first convex rubber plug, and a convex protrusion of the first convex rubber plug is embedded in the throat structure; and/or a sealing convex ring (611) is arranged on the outer circumferential wall of the second rubber plug (61); and/or a first stop ring (21) which is convex towards the radial inner side of the accommodating bottle body (2) is arranged on the inner circumferential wall of the second end of the accommodating bottle body (2), and the inner circle diameter of the ring body of the first stop ring (21) is smaller than the outer circle diameter of the second rubber plug (61).

5. The microfluidic device according to claim 4, wherein the second rubber plug (61) is a second embossed rubber plug, and the shape of the embossed projection of the second embossed rubber plug can match the shape of the necking structure, so as to completely release and discharge the material in the inner sealed containing space through the needle port (41).

6. The microfluidic device according to claim 1, wherein the end of the vial sleeve (3) remote from the microfluidic chip body (1) has a second stop ring (31) protruding radially inward along it; and/or the needle opening (41) is arranged on the circumferential side wall of the needle body (4).

7. The microfluidic device according to any of claims 2 to 6, wherein there are three receiving vials (2), three receiving vials (2) are adjacently arranged, and the microfluidic channel (11) is capable of communicating the three receiving vials (2).

8. A blending control method of a microfluidic device, which is used for controlling the microfluidic device for realizing material blending of any one of claims 1 to 6, and comprises the following steps:

controlling to apply axial force to the second sealing components respectively arranged on the two accommodating bottle bodies (2) to enable the accommodating bottle bodies (2) to move close to the needle body (4) and enable the needle body (4) to be respectively converted from the first sealing state to the circulating state;

and controlling to release the axial force applied to the second sealing component of the second bottle-containing body of the two bottle-containing bodies (2), continuously applying the axial force to the second sealing component of the first bottle-containing body of the two bottle-containing bodies (2), releasing the axial force applied to the first bottle-containing body before the needle body (4) corresponding to the second bottle-containing body is converted from the flow-through state to the second sealing state, and applying the axial force to the second bottle-containing body, so that the axial force is applied alternately after the axial force is applied to the first bottle-containing body and the second bottle-containing body for a preset number of times, so that the needle body (4) of one of the first bottle-containing body and the second bottle-containing body is converted from the flow-through state to the second sealing state.

9. A blending control method of a microfluidic device, which is used for controlling the microfluidic device for realizing material blending of claim 7, and comprises the following steps:

controlling to apply axial force to second sealing components respectively arranged on a first accommodating bottle body and a second accommodating bottle body in the three accommodating bottle bodies (2) to enable the accommodating bottle bodies (2) to move close to the needle body (4) and enable the needle body (4) to be respectively converted from the first sealing state to the circulating state;

controlling to release the axial force applied to the second sealing component of the second accommodating bottle body, continuously applying the axial force to the second sealing component of the first accommodating bottle body, releasing the axial force applied to the first accommodating bottle body before the needle body (4) corresponding to the second sealing component is converted from the circulation state to the second sealing state, and applying the axial force to the second accommodating bottle body, so that the axial force is alternately and respectively applied to the first accommodating bottle body and the second accommodating bottle body for preset times, and then applying the axial force to convert the needle body (4) of one of the first accommodating bottle body and the second accommodating bottle body from the circulation state to the second sealing state;

after the needle body (4) of one of the first accommodating bottle body and the second accommodating bottle body is converted into the second sealing state from the circulation state, the axial force applied to the first accommodating bottle body or the second sealing component of the second accommodating bottle body is controlled to be released, the second sealing component applied to the third accommodating bottle body of the three accommodating bottle bodies (2) is controlled to enable the accommodating bottle body (2) to move close to the needle body (4), the needle body (4) corresponding to the third accommodating bottle body is converted into the circulation state from the first sealing state, the axial force applied to the third accommodating bottle body is released before the needle body (4) of the third accommodating bottle body is converted into the second sealing state, and the axial force is applied to the second sealing component of one of the first accommodating bottle body and the second accommodating bottle body in the circulation state again, after the times are alternately preset, axial force is exerted to enable one bottle body which can enable materials in the first containing bottle body and the second containing bottle body to flow through and the needle body (4) of one of the third containing bottle bodies to be converted into a second sealing state from a flowing state.

Technical Field

The invention belongs to the technical field of consumable materials for biological experiments, and particularly relates to a micro-fluidic device for realizing material mixing and a mixing control method.

Background

Molecular diagnosis is a comprehensive and comprehensive clinical diagnosis technology which detects contents, types and coding information of biomolecules including nucleic acids, proteins, saccharides and other substances in a human body and combines related contents of biomolecular science, and is mainly applied to the fields of diagnosis of genetic diseases, control of infectious diseases and tumor treatment at present. However, the sample collected from the patient is generally complicated and contains many substances that inhibit or interfere with the detection, and therefore, many pretreatment processes are required to purify the substance to be detected and perform qualitative or quantitative detection. The conventional molecular diagnosis process generally transfers clinical samples to a detection laboratory with related qualification, depends on related equipment of the laboratory and professional operators to perform pretreatment of the samples, and is uniformly operated to perform biochemical detection or analysis. In the process, a long time is delayed, and the detection of some sudden diseases, large-scale infectious diseases and complex diseases is difficult to carry out in real time and in place. In addition, such a testing laboratory requires expensive testing equipment and specialized laboratory staff, and is difficult to be popularized in resource-poor areas.

The advent of microfluidic technology has addressed some of the problems in molecular diagnostics, and microfluidic chips have miniaturized the modules of biochemical assays and integrated them together via microchannels. The chip realizes the operations of accurate distribution of fluid, heating of reactants, uniform mixing of reagents, fluorescence detection and the like under the control of a matched instrument, thereby completing the complex, time-consuming and labor-consuming detection in the traditional laboratory at low cost in a short time and realizing the real 'sample input-result output'. However, in order to realize high-efficiency amplification on a chip, one of the important difficulties is to mix reagents uniformly, and generally, the whole chip is totally enclosed, and the channels of the chip are narrow, so that the reagents to be mixed uniformly are less, and the uniformity of the reagents is difficult to ensure, and thus the detection accuracy cannot be ensured. Therefore, only by controlling the movement of the fluid, the sufficient and efficient mixing of the materials among the steps can be ensured, and the problems of high cost, poor sensitivity, complex structure, excessively complex operation and related detection equipment and the like in the prior art can be avoided.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to provide a microfluidic control device and a mixing control method for realizing material mixing, wherein the accommodating bottle bodies and the microfluidic chip are integrated into a whole, and materials in the two accommodating bottle bodies can flow between each other to realize uniform mixing of the materials, so that the accuracy of a detection result is ensured.

In order to solve the above problems, the present invention provides a microfluidic device for uniformly mixing materials, comprising a microfluidic chip body and at least two containing bottle bodies for containing different materials, wherein a microfluidic channel is configured in the microfluidic chip body, the at least two containing bottle bodies are respectively located in a bottle sleeve disposed on the microfluidic chip body, and are respectively and correspondingly provided with a needle body having a needle opening, the two containing bottle bodies can be communicated with the microfluidic channel through the needle body, the first end and the second end of the containing bottle body in the axial direction are respectively connected with a first sealing component and a second sealing component to form an inner sealed containing space of the containing bottle body, and the needle body has a first sealed state where the needle opening is located in the first sealing component, and a circulating state where the needle opening is located in the inner sealed containing space, the containing bottle body can move linearly along the axial direction of the bottle sleeve under the action of axial force so that the needle body is converted into a circulation state from a first sealing state, and the second sealing assembly can be close to the first sealing assembly under the action of the axial force so that the material is released.

Preferably, the needle body further has a second sealing state of the needle opening in the second sealing assembly, and the second sealing assembly is further capable of switching the needle body from the flow-through state to the second sealing state under the action of the axial force.

Preferably, the first sealing assembly comprises a first rubber plug and a protective cover, and when the needle body is in the first sealing state, the needle opening is in the first rubber plug; the second sealing component comprises a second rubber plug and a hard gasket positioned at one side of the second rubber plug, which deviates from the first rubber plug, and the needle body is positioned in the second rubber plug when in the second sealing state.

Preferably, the first end is of a throat structure, the first rubber plug is a first convex rubber plug, and a convex protrusion of the first convex rubber plug is embedded in the throat structure; and/or a sealing convex ring is arranged on the outer circumferential wall of the second rubber plug; and/or a first stop ring which is convex towards the radial inner side of the accommodating bottle body is arranged on the inner circumferential wall of the second end of the accommodating bottle body, and the inner circle diameter of a ring body of the first stop ring is smaller than that of the second rubber plug.

Preferably, the second rubber plug is a second convex-shaped rubber plug, and the shape of the convex-shaped protruding part of the second convex-shaped rubber plug can be matched with the shape of the necking structure, so that the materials in the inner sealed containing space are completely released and discharged through the needle opening.

Preferably, one end of the bottle sleeve, which is far away from the microfluidic chip body, is provided with a second stop ring which is convex inwards along the radial direction of the bottle sleeve; and/or the needle opening is positioned on the circumferential side wall of the needle body.

Preferably, the number of the accommodating bottle bodies is three, the three accommodating bottle bodies are arranged adjacently, and the microfluidic pipeline can communicate the three accommodating bottle bodies.

The invention also provides a mixing control method of the microfluidic device, which is used for controlling the microfluidic device for realizing the material mixing, and comprises the following steps:

controlling to apply axial force to the second sealing assemblies respectively arranged on the two accommodating bottle bodies to enable the accommodating bottle bodies to move close to the needle bodies and enable the needle bodies to be respectively converted from the first sealing state to the circulation state;

and controlling to release the axial force applied to the second sealing component of the second accommodating bottle body of the two accommodating bottle bodies, continuously applying the axial force to the second sealing component of the first accommodating bottle body of the two accommodating bottle bodies, releasing the axial force applied to the first accommodating bottle body before the needle body corresponding to the second accommodating bottle body is converted from the circulation state to the second sealing state, and applying the axial force to the second accommodating bottle body, so that the axial force is alternately applied to the first accommodating bottle body and the second accommodating bottle body for a preset number of times, and then applying the axial force to convert the needle body of one of the first accommodating bottle body and the second accommodating bottle body from the circulation state to the second sealing state.

The invention also provides a mixing control method of the microfluidic device, which is used for controlling the microfluidic device for realizing the material mixing, and comprises the following steps:

the axial force is controlled to be exerted on a second sealing assembly respectively arranged on a first accommodating bottle body and a second accommodating bottle body in the three accommodating bottle bodies, so that the accommodating bottle bodies can move close to the needle bodies, and the needle bodies are respectively converted into a circulation state from the first sealing state;

controlling to release the axial force applied to the second sealing component of the second accommodating bottle body, continuously applying the axial force to the second sealing component of the first accommodating bottle body, releasing the axial force applied to the first accommodating bottle body before the needle body corresponding to the second sealing component is converted from the flow state to the second sealing state, and applying the axial force to the second accommodating bottle body, so that the axial force is alternately and respectively applied to the first accommodating bottle body and the second accommodating bottle body for a preset number of times, and then applying the axial force to convert the needle body of one of the first accommodating bottle body and the second accommodating bottle body from the flow state to the second sealing state;

after the needle body of one of the first accommodating bottle body and the second accommodating bottle body is converted from the circulation state to the second sealing state, controlling to release the axial force applied to the second sealing component of the first accommodating bottle body or the second accommodating bottle body, controlling to apply the axial force to the second sealing component of the third accommodating bottle body so that the accommodating bottle body can move close to the needle body, converting the needle body corresponding to the third accommodating bottle body from the first sealing state to the circulation state, and before the needle body of the third accommodating bottle body is converted from the circulation state to the second sealing state, releasing the axial force applied to the third accommodating bottle body and applying the axial force to the second sealing component of the first accommodating bottle body and the second accommodating bottle body in which the needle body is in the circulation state again, after the times are alternately preset, axial force is applied to enable one bottle body which can enable materials in the first containing bottle body and the second containing bottle body to flow through and one needle body in the third containing bottle body to be converted into a second sealing state from a flowing state.

According to the micro-fluidic device for realizing material mixing and the mixing control method, the accommodating bottle body and the second sealing component can move close to the needle body under the action of the axial force, and the needle body is switched from the first sealing state to the circulation state in the moving process, so that the material is released into the micro-fluidic pipeline from the internal sealing accommodating space and enters another accommodating bottle body with different materials through the micro-fluidic pipeline, the integrated design between the micro-fluidic chip and the accommodating bottle body is realized, the automatic mixing of different materials is realized, the uniform mixing can be realized on different accommodating bottle bodies by alternately applying the axial force for multiple times, the accuracy of a detection result is further ensured, and the automation degree of the micro-fluidic device is improved.

Drawings

Fig. 1 is a schematic partial structural view of a microfluidic device for achieving material blending according to an embodiment of the present invention, in which a needle is shown in a first sealing state;

FIG. 2 is an enlarged partial schematic view of FIG. 1;

FIG. 3 is a schematic view of a structure of the needle body of FIG. 1;

FIG. 4 is another schematic view of the needle body of FIG. 1;

FIG. 5 is a further schematic view of the needle body of FIG. 1;

fig. 6 shows the state change of the accommodating bottle body in the microfluidic device for achieving material mixing according to the embodiment of the present invention after being subjected to an axial force, wherein (a) the needle body is in a first sealing state, (b) and (c) the needle body is in a flow state, and (d) the needle body is in a second sealing state, and this process achieves that the material in the accommodating bottle body is released and flows out under the axial force and is finally sealed again;

fig. 7 to 12 are schematic diagrams showing the state of each accommodating bottle body in the process of mixing different materials in two accommodating bottle bodies under the condition that two accommodating bottle bodies are arranged in the microfluidic device for realizing material mixing, wherein the material in the bottle B is soluble solid T, and the material in the bottle a is liquid S;

fig. 13 to 17 show the state of each accommodating bottle body in the process of mixing different materials in the three accommodating bottle bodies in the case that three accommodating bottle bodies are arranged in the microfluidic device for realizing material mixing, wherein the material in the bottle B is soluble solid T, the material in the bottle C is soluble solid T1, and the material in the bottle a is liquid S.

The reference numerals are represented as:

1. a microfluidic chip body; 11. a microfluidic conduit; 2. receiving a bottle body; 21. a first stop ring; 3. a bottle sleeve; 31. a second stop ring; 4. a needle body; 41. a needle opening; 51. a first rubber plug; 52. a protective cover; 61. a second rubber plug; 611. a sealing convex ring; 62. a hard gasket.

Detailed Description

Referring to fig. 1 to 17 in combination, according to an embodiment of the present invention, a microfluidic device for uniformly mixing materials is provided, which includes a microfluidic chip body 1 and at least two containing bottle bodies 2 for containing different materials (one of the materials needs to be in a liquid state), a microfluidic pipeline 11 is configured in the microfluidic chip body 1, the at least two containing bottle bodies 2 are respectively located in a bottle sleeve 3 disposed on the microfluidic chip body 1, the two containing bottle bodies 2 are respectively and correspondingly provided with a needle body 4 having a needle opening 41, the two containing bottle bodies 2 can be communicated with the microfluidic pipeline 11 through the needle body 4, a first sealing component and a second sealing component are respectively connected to a first end and a second end of the containing bottle body 2 in an axial direction to form an inner sealed containing space of the containing bottle body 2, the needle body 4 has a first sealing state that the needle opening 41 is in the first sealing assembly, the needle opening 41 is in a flow state in the internal sealing accommodating space, the accommodating bottle body 2 can move linearly along the axial direction of the bottle sleeve 3 under the action of axial force to enable the needle body 4 to be converted from the first sealing state into the flow state, and the second sealing assembly can approach the first sealing assembly under the action of the axial force to enable the materials to be released, in particular, to enable the materials in one of the two accommodating bottle bodies 2 to be released into the other to achieve material mixing. In the technical scheme, the containing bottle body and the second sealing component can be close to the needle body under the action of the axial force to move, and in the moving process, the needle body is switched to a circulation state from a first sealing state, so that the materials are released into the micro-fluidic pipeline from the internal sealing containing space and enter into another containing bottle body 2 with different materials through the micro-fluidic pipeline, the automatic mixing of different materials is realized while the integrated design between the micro-fluidic chip and the containing bottle body is realized, the uniform mixing can be realized on different containing bottle bodies 2 by alternately applying the axial force for many times, the accuracy of a detection result is further ensured, and the automation degree of the micro-fluidic device is improved.

It should be noted that the bottle sleeve 3 can restrain the radial displacement of the accommodating bottle body 2 without limiting the axial displacement of the accommodating bottle body 2.

In some embodiments, the needle body 4 further has a second sealing state in which the needle port 41 is located in the second sealing assembly, and the second sealing assembly is further capable of finally switching the needle body 4 from the flow-through state to the second sealing state under the action of the axial force, and in particular, after the material in the accommodating bottle body 2 is completely released, the needle body 4 can be located in the second sealing state, that is, the needle port 41 is sealed again, which is capable of adapting to a situation that the microfluidic device has a plurality of accommodating bottle bodies 2, and the release of the internal material has a certain sequence, so as to prevent the material in other accommodating bottle bodies from being released into the released accommodating bottle body without entering or only partially entering the accommodating bottle body 2 that needs to enter.

The specific structural forms of the first sealing component and the second sealing component are various, but it should be noted that the first sealing component may be specifically implemented in a fixed sealing manner due to the absence of the requirement for displacement, specifically, for example, the first sealing component includes a first rubber plug 51 and a protective cover 52, the protective cover 52 may be an aluminum cover (the specific material is selected based on the principle that the needle 4 can be smoothly punctured), and at this time, when the needle 4 is in the first sealing state, the needle opening 41 is located in the first rubber plug 51. The second sealing component comprises a second rubber plug 61 and a hard gasket 62 which is arranged on one side of the second rubber plug 61, wherein the second rubber plug 61 deviates from the first rubber plug 51, the needle body 4 is arranged in the second sealing state, the needle opening 41 is arranged in the second rubber plug 61, the hard gasket 62 has certain rigidity, and the deformation of the hard gasket is small so as to ensure the effective transmission of the axial force.

In some embodiments, the first end is a throat structure, the first rubber plug 51 is a first rubber plug with a convex shape, and the convex protrusion of the first rubber plug is embedded in the throat structure, so that the first rubber plug with a convex shape is axially positioned in the accommodating bottle body 2 through the structure of the first rubber plug, and the second rubber plug 61 is a second rubber plug with a convex shape, and the shape of the convex protrusion of the second rubber plug can be matched with the shape of the throat structure, so that the material in the inner sealed accommodating space is completely released and discharged through the needle opening 41, and the utilization rate of the material is improved.

In order to effectively prevent the leakage of the material, preferably, a sealing convex ring 611 is arranged on the outer circumferential wall of the second rubber plug 61, and a plurality of sealing convex rings 611 can be arranged at intervals along the axial direction of the second rubber plug 61.

In some embodiments, the inner circumferential wall of the second end of the bottle containing body 2 has a first stop ring 21 protruding toward the inner side of the bottle containing body 2, and the inner circular diameter of the ring body of the first stop ring 21 is smaller than the outer circular diameter of the second rubber plug 61, so as to prevent the second rubber plug 61 from falling out of the bottle containing body 2 from the second end, and effectively prevent the material leakage. The end of the bottle sleeve 3 away from the microfluidic chip body 1 is provided with a second stop ring 31 protruding inwards along the radial direction, so that the accommodating bottle body 2 and all parts assembled with the accommodating bottle body can be limited in the bottle sleeve 3 and prevented from falling out.

One end of the bottle sleeve 3 close to the microfluidic chip body 1 is connected with the microfluidic chip body 1 in a buckling or ultrasonic bonding mode after the accommodating bottle body 2 is arranged in the bottle sleeve 3.

The needle port 41 can be opened at the top end of the needle body 4, and preferably, the needle port 41 is located on the circumferential side wall of the needle body 4, as shown in fig. 3 to 5, so when the needle body 4 is in the first sealing state and the second sealing state, if the needle body receives the reverse thrust pressure of the material in the microfluidic pipeline 11, the needle body can extrude the side wall of the rubber plug, an upward force cannot be generated, and then the rubber plug is bounced to cause leakage, that is, the rubber plug can be bounced when the material is reversely pushed, thereby ensuring the sealing effect of the needle port 41 and effectively preventing the leakage of the material.

In some embodiments, the number of the receiving bottle bodies 2 is three or more, three or more receiving bottle bodies 2 are adjacently arranged, and the microfluidic channel 11 can communicate the receiving bottle bodies 2, so that uniform mixing of more different materials can be realized.

According to an embodiment of the present invention, as shown in fig. 7 to 12, there is also provided a blending control method of a microfluidic device for controlling a microfluidic device having two accommodating bottle bodies 2 to achieve blending of materials, the method including:

controlling the second sealing components respectively arranged on the two accommodating bottle bodies 2 to apply axial force to enable the accommodating bottle bodies 2 to move close to the needle body 4 and enable the needle body 4 to be respectively converted from the first sealing state to the circulating state;

and controlling to release the axial force applied to the second sealing component of the second one of the two accommodating bottle bodies 2, and to continue to apply the axial force to the second sealing component of the first one of the two accommodating bottle bodies 2, and to release the axial force applied to the first one of the two accommodating bottle bodies before the needle body 4 corresponding to the second one of the two accommodating bottle bodies is converted from the flow-through state to the second sealing state, and to apply the axial force to the second one of the two accommodating bottle bodies, so that the axial force is applied alternately after the axial force is applied to the first one of the two accommodating bottle bodies and the second one of the two accommodating bottle bodies for a predetermined number of times, so that the needle body 4 of one of the first one accommodating bottle body and the second one of the two accommodating bottle bodies is converted from the flow-through state to the second sealing state.

According to an embodiment of the present invention, referring to fig. 13 to 17, there is provided a blending control method for a microfluidic device, for controlling the microfluidic device for blending materials, including:

controlling the axial force applied to the second sealing components respectively arranged on the first accommodating bottle body and the second accommodating bottle body in the three accommodating bottle bodies 2 to enable the accommodating bottle bodies 2 to move close to the needle body 4 and enable the needle body 4 to be respectively converted from the first sealing state to the circulating state;

controlling to release the axial force applied to the second sealing component of the second accommodating bottle body, continuously applying the axial force to the second sealing component of the first accommodating bottle body, releasing the axial force applied to the first accommodating bottle body before the needle body 4 corresponding to the second sealing component is converted from the flow state to the second sealing state, and applying the axial force to the second accommodating bottle body, so that the axial force is alternately and respectively applied to the first accommodating bottle body and the second accommodating bottle body for a preset number of times, and then applying the axial force to convert the needle body 4 of one of the first accommodating bottle body and the second accommodating bottle body from the flow state to the second sealing state;

after the needle 4 of one of the first and second receiving bottles is switched from the flow-through state to the second sealing state, controlling to release the axial force applied to the second sealing member of the first or second receiving bottle, controlling to apply the axial force to the second sealing member of the third of the three receiving bottles 2 so that the receiving bottle 2 can move close to the needle 4 and the needle 4 corresponding to the third receiving bottle is switched from the first sealing state to the flow-through state, and before the needle 4 of the third receiving bottle is switched from the flow-through state to the second sealing state, releasing the axial force applied to the third receiving bottle and applying the axial force again to the second sealing member of the one of the first and second receiving bottles in which the needle 4 is in the flow-through state, after the preset times, axial force is applied to make one bottle body which can make the materials in the first accommodating bottle body and the second accommodating bottle body circulate and the needle body 4 of one of the third accommodating bottle bodies change from a circulating state to a second sealing state.

It will be understood that the first receiving flask, now relieved of its force, has sufficient space for the second sealing member to move up under the action of the material entering it, whereas in theory the volume of the first receiving flask should be greater than the total volume of material in both receiving flasks, so as to achieve mixing of the material in the first receiving flask.

Example 1:

the process of mixing the contents with the two receiving flask 2 is further described below with reference to fig. 7 to 12:

before the device is not used, when the bottle A and the bottle B are placed in the bottle sleeve (namely, the bottle sleeve 3, the same below), the needle (namely, the needle body 4, the same below) just pierces a part of a rubber plug (namely, the first rubber plug 51, the same below) of a bottle opening, so that the tail end of the needle (namely, the needle opening 41, the same below) is sealed, and meanwhile, a fluid pipeline (namely, the microfluidic pipeline 11, the same below) at the lower part of the bottle sleeve is sealed. But the needle can not puncture the rubber plug and does not damage the sealing performance of the biological materials in the bottle body, namely the needle is positioned in the rubber plug. Meanwhile, in order to make the fluid in the bottles A and B have a flowing space, the rubber plug of the bottle A or the bottle B is pre-assembled at a position close to the bottle mouth, and here, the rubber plug at the bottom of the bottle B is pre-assembled at a position close to the bottom of the bottle B, as shown in figure 7.

When the material blending device is started, the upper parts of the bottle A and the bottle B are sequentially subjected to downward force (namely, the axial force and the downward force), so that the bottle stopper is punctured by the needle, and two outlets for biological materials to be blended (namely, the material S and the material T) are opened, as shown in fig. 8.

Continue to exert pressure to A bottle bottom, because whole bottle is the restriction inside the bottle cover, the export of two bottles that are equipped with different biological material respectively simultaneously also is punctured by the needle and opens, at this moment, the plug at the bottom of A bottle is under the drive of downward pressure, can slow moving, discharge the inside material of A bottle simultaneously, because the plug of B bottle also is punctured this moment, the plug of B bottle is located the nearer position department of bottleneck, the space of activity that makes progress has, consequently the material in the A bottle is under the drive of pressure, get into B bottle and carry out the mixing with its material, the bottom plug of B bottle also can the rebound simultaneously, its process is as shown in figure 9.

The liquid in the bottle A is completely (or partially) injected into the bottle B, the bottom rubber plug of the bottle A is lowered to a position close to the bottle mouth at this time, and the needle is not pricked into the bottom rubber plug at the time, as shown in fig. 10.

The mixed material in the bottle B is driven into the bottle A, and the process is similar to the process of driving the bottle A into the bottle B, as shown in fig. 11 and 12. The above process can be repeated for a plurality of times according to the requirement, so that the materials of the bottle A and the bottle B can be fully mixed. It is noted that at least one of the materials in the bottles A and B is in a liquid state.

Example 2:

the process of mixing the materials with three containing bodies 2 is further described below with reference to fig. 13 to 17:

before the device is not used, when the bottle A (the material in the bottle A is S), the bottle B (the material in the bottle B is T1) and the bottle C (the material in the bottle C is T) are placed inside the bottle sleeve, the needle just pricks into one part of the rubber plug of the bottle mouth, the tail end of the needle is sealed, and meanwhile, the fluid pipeline at the lower part of the bottle sleeve is sealed. But the needle can not puncture the rubber plug and does not damage the sealing performance of the biological materials in the bottle body, namely the needle is positioned in the rubber plug. As shown in fig. 13.

When this material mixing device is started, at first, A bottle and B bottle upper portion receive decurrent power in proper order for the bottle plug is punctured to the needle, opens the export of waiting to mix biological material, and the material export of C bottle still is in the closed condition this moment. As shown in fig. 14. Continue to exert pressure to A bottle bottom, because whole bottle is the restriction inside the bottle cover, the export of two bottles that are equipped with different biological material respectively simultaneously also is punctured by the needle and opens, at this moment, the plug at the bottom of A bottle is under the drive of downward pressure, can slow moving, discharge the inside material of A bottle simultaneously, because the plug of B bottle also is punctured this moment, the plug of B bottle is located the nearer position department of bottleneck, the space of activity that makes progress has, consequently the material in the A bottle is under the drive of pressure, get into B bottle and carry out the mixing with its material, the bottom plug of B bottle also can the rebound simultaneously, its process is as shown in figure 14.

The blending process is consistent with the blending process of two materials, after the blending process is finished, the bottom rubber plug of one material bottle is pressed to the bottle mouth, and the needle head is pricked into the bottom rubber plug, so that the bottom rubber plug is sealed. Here the needle is inserted into the plug at the bottom of the a vial, which achieves the seal, as shown in fig. 15.

And then, applying pressure to the bottom of the bottle C to puncture the rubber plug of the bottle opening by a needle, opening an outlet for the biological material to be uniformly mixed, communicating the bottle B with the bottle C, and closing the bottle A as shown in fig. 16.

The blending process of the bottle B and the bottle C is similar to the previous blending process, after the blending of the bottle B and the bottle C is finished, all blending liquid is injected into the bottle C, and the bottle B is closed at the same time, as shown in fig. 17.

It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.