阅读说明：本技术 一种n-苄氧羰基-3-碘-l-丙氨酸甲酯的合成方法 (Synthesis method of N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester ) 是由 徐红岩 马敬祥 于 2021-10-28 设计创作，主要内容包括：本发明涉及一种N-苄氧羰基-3-碘-L-丙氨酸甲酯的合成方法。主要解决N-苄氧羰基-3-碘-L-丙氨酸甲酯合成和放大生产的技术问题。本发明合成方法包括以下步骤：在二氯甲烷溶液中,N-苄氧羰基-L-丝氨酸甲酯和甲基磺酰氯反应,加入三乙胺除去副产物氯化氢,生成化合物1；在丙酮溶液中,化合物1和碘化钠反应,加热回流反应,得到的粗产品用正庚烷重结晶纯化,生成目标化合物2。作为手性氨基酸砌块,N-苄氧羰基-3-碘-L-丙氨酸甲酯可以通过亲核取代反应、金属钯催化的偶联反应,选择性地合成具有光学活性的非天然α-氨基酸衍生物,特别是苯丙氨酸系列化合物,用于具有多肽药物的研发。(The invention relates to a synthetic method of N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester. Mainly solves the technical problems of synthesis and scale-up production of N-carbobenzoxy-3-iodine-L-alanine methyl ester. The synthesis method comprises the following steps: in a dichloromethane solution, reacting N-benzyloxycarbonyl-L-serine methyl ester with methylsulfonyl chloride, adding triethylamine to remove a byproduct hydrogen chloride, and generating a compound 1; reacting the compound 1 with sodium iodide in an acetone solution, heating and refluxing for reaction, and recrystallizing and purifying the obtained crude product with n-heptane to obtain the target compound 2. As a chiral amino acid block, N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester can selectively synthesize an unnatural alpha-amino acid derivative with optical activity, in particular to a phenylalanine series compound, through a nucleophilic substitution reaction and a coupling reaction catalyzed by metal palladium, and is used for research and development of polypeptide drugs.)

1. A synthetic method of N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester is characterized by comprising the following steps of: the method comprises the following steps: the first step, in a dichloromethane solution, reacting N-carbobenzoxy-L-serine methyl ester with methylsulfonyl chloride, adding triethylamine to remove a byproduct hydrogen chloride, and generating a compound 1; secondly, reacting the compound 1 with sodium iodide in an acetone solution, heating and refluxing for reaction, and recrystallizing and purifying the obtained crude product with n-heptane to generate a target compound 2; the synthesis route is as follows:

。

2. the method for synthesizing N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester according to claim 1, wherein: in the first step, triethylamine is added in an equivalent amount to the methanesulfonyl chloride.

3. The method for synthesizing N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester according to claim 1, wherein: the second reaction time was 2 hours.

4. The method for synthesizing N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester according to claim 1, wherein: and in the second step, adding petroleum ether/ethyl acetate and silica gel into the crude product, and stirring at room temperature.

5. The method for synthesizing N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester according to claim 4, wherein: in the second step, the volume ratio of petroleum ether to ethyl acetate is 5: 1.

Technical Field

The invention relates to a synthetic method of N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester (CAS: 56877-38-4).

Background

Amino acids are the most basic substances constituting life proteins, and include natural amino acids and unnatural amino acids. With the development of polypeptide drugs, natural amino acids have failed to meet the requirements of research and application, while unnatural amino acids can be designed according to human needs. The optically active unnatural amino acid is a chiral synthesis unit of some novel bioactive polypeptides, the research on the synthesis of the optically active unnatural amino acid is also an effective tool for exploring the structure, the function and the functional polypeptide of the protein, and the optically active unnatural amino acid has extremely important academic research and application values.

The source of the unnatural amino acid is generally obtained by obtaining a racemic amino acid precursor by a chemical synthesis method and then resolving the racemic amino acid precursor. The chemical synthesis method comprises the following steps: strecker synthesis, Gabriel synthesis, malonate synthesis, Knoop-oesterlin synthesis, hydantoin intermediate ring opening, and the like. The racemates are generally obtained chemically and must be resolved to obtain optically pure amino acids. The resolution method comprises a crystallization method, a chemical resolution method, an extraction resolution method, a biological enzyme resolution method, a membrane separation resolution method, a chromatographic resolution method, a capillary electrophoresis resolution method and the like. Although chiral amino acids can be obtained by resolution, the operation is complicated and the yield is limited. In recent years, asymmetric synthesis techniques have become an important component of organic synthetic chemistry. More and more chiral amino acids are obtained through asymmetric synthesis, and the research content of organic synthesis methodology is greatly enriched. However, the asymmetric synthesis often requires a high-selectivity catalyst, and the activity, stability, controllability, price and the like of the catalyst are factors influencing the further industrialization of the asymmetric synthesis technology.

Serine is a natural amino acid, and has rich source and low cost. Serine is used as a raw material, protected amino acid with iodo end is obtained through conversion, the protected amino acid can be used as a chiral amino acid block, unnatural alpha-amino acids with different structure types are obtained through coupling reaction or SN2 reaction, and the selective synthesis of the unnatural amino acids is realized.

Disclosure of Invention

The invention mainly aims to provide a method for synthesizing N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester, which mainly solves the technical problems of synthesis and scale-up production of the N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester.

The technical scheme of the invention is as follows: a synthetic method of N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester is characterized by comprising the following steps of: the first step, in a dichloromethane solution, reacting N-carbobenzoxy-L-serine methyl ester with methylsulfonyl chloride, adding triethylamine to remove a byproduct hydrogen chloride, and generating a compound 1; secondly, reacting the compound 1 with sodium iodide in an acetone solution, heating and refluxing for reaction, and recrystallizing and purifying the obtained crude product with n-heptane to generate a target compound 2; the synthesis route is as follows:

。

in the first step, triethylamine which is equivalent to methanesulfonyl chloride is added to remove hydrogen chloride generated in the reaction; in the second step, the reaction time is 2 hours; after the reaction is finished, adding petroleum ether/ethyl acetate (volume ratio is 5: 1) and silica gel into the obtained crude product, stirring at room temperature, purifying, and recrystallizing the product with n-heptane.

The invention has the beneficial effects that: the raw materials used in the invention are easy to obtain, the price is low, the reaction condition is mild, and the intermediate and the final product do not need to be purified by a chromatographic column, so that the method is very suitable for the large-scale production of the N-benzyloxycarbonyl-3-iodo-L-alanine methyl ester in a factory. The N-carbobenzoxy-3-iodine-L-alanine methyl ester can be used as a chiral amino acid building block for synthesizing unnatural alpha-amino acid, in particular to a phenylalanine series compound with high additional value.

Detailed Description

Step 1:

N-benzyloxycarbonyl-L-serine methyl ester (700 g, 2.76 mol), methanesulfonyl chloride (357 g, 3.11 mol) and dichloromethane (4L) were added to a 10L four-necked flask, the temperature was cooled to 0-5 ℃ in an ice bath under nitrogen protection, and triethylamine (322 g, 3.11 mol) was added dropwise. After the dropwise addition, the mixture was stirred at 0 to 10 ℃ for 2 hours. Quench with water (3L), allow to separate by settling, wash the organic phase with 1N HCl (3L), and concentrate the organic phase under reduced pressure to give compound 1 (905 g, 2.73 mol, 99%) as a pale yellow oil. LC-MS (ESI) M/z 332.09 [ M + H [ ]]⁺ 。

Step 2:

in a 10L four-necked flask, Compound 1 (905 g, 2.73 mol), acetone (5L) and sodium iodide (1)2 kg, 8.00 mol), heated at reflux for 2 hours. The reaction solution was cooled to room temperature, filtered, the filter cake was rinsed with acetone (1L), the filtrate was concentrated under reduced pressure, then ethyl acetate (6L) was added to dissolve, and washed with a saturated aqueous sodium sulfite solution (2L X3) and a saturated brine (3L), respectively, and the organic phase was concentrated under reduced pressure to give 990 g of a crude product. Adding a petroleum ether/ethyl acetate solution (volume ratio is 5:1, 15L) and silica gel (1 kg), stirring at room temperature for 1 hour, performing suction filtration, concentrating the filtrate under reduced pressure to obtain a crude product, adding n-heptane (6L), stirring at 60 ℃ until the crude product is clear, stirring at room temperature overnight, performing suction filtration, leaching with n-heptane (500 mL), and drying the filter cake to obtain a white solid, namely the target compound 2 (842 g, 2.32 mol, 85%).¹H NMR (400 MHz, CD₃Cl) 7.36-7.29 （m, 5H），5.62-5.51（m, 1H），5.11-5.05（m, 2H），4.59-4.52（m, 1H），3.80（s, 3H），3.59-3.53（m, 2H）；LC-MS (ESI): m/z 385.99 [M+Na]⁺。