阅读说明：本技术 一种铁皮石斛组培苗的清洗液及其清洗方法 (Cleaning solution and cleaning method for dendrobium officinale tissue culture seedlings ) 是由 曹瑞钦 马小凤 吴飞云 陈辉 于 2021-08-27 设计创作，主要内容包括：本发明公开了一种铁皮石斛组培苗的清洗液及其清洗方法,包括所述按照份量份计,所述清洗液的组成成分为：清水45-135份,烷基芳基磺酸钠0.5-1.5份,磷酸三钠2-6份,硅酸钠0.25-0.75份,硫酸钠5.25份,羧基甲纤维素0.1-0.3份,牛油脂肪酸硫酸钠0.4-1.2份,本发明通过将在铁皮石斛植株上切下长2～5cm的幼嫩茎段在流水下冲洗；用刷子蘸取少许清洗液进行轻轻刷洗,最后用无菌水冲洗5～6次,从而使得铁皮石斛组培苗的清洁更加便捷,避免因为铁皮石斛组培苗的清洁不彻底导致琼脂清洁不干净,降低因为琼脂发霉引起的烂根概率,增加更加的清洁效率,避免导致铁皮石斛组培苗因为清洁时间多长造成额的损伤,增加铁皮石斛组培苗的清洁效率。(The invention discloses a cleaning solution for dendrobium officinale tissue culture seedlings and a cleaning method thereof, wherein the cleaning solution comprises the following components in parts by weight: 45-135 parts of clean water, 0.5-1.5 parts of sodium alkyl aryl sulfonate, 2-6 parts of trisodium phosphate, 0.25-0.75 part of sodium silicate, 5.25 parts of sodium sulfate, 0.1-0.3 part of carboxyl methylcellulose and 0.4-1.2 parts of tallow fatty acid sodium sulfate, wherein young stem sections with the length of 2-5cm cut from dendrobium officinale plants are washed under running water; dip in with the brush and get a little washing liquid and scrub gently, wash 5~6 times with aseptic water at last to make the cleanness of dendrobium officinale tissue culture seedling more convenient, avoid leading to agar clean not thoroughly because the cleanness of dendrobium officinale tissue culture seedling, reduce because the rotten root probability that agar mildewed, increase more clean efficiency, avoid leading to the damage of dendrobium officinale tissue culture seedling because the clean time is long leads to the volume, increase the clean efficiency of dendrobium officinale tissue culture seedling.)

1. The cleaning solution for the dendrobium officinale tissue culture seedlings and the cleaning method thereof are characterized in that the cleaning solution comprises the following components in parts by weight: 45-135 parts of clean water, 0.5-1.5 parts of alkyl aryl sodium sulfonate, 2-6 parts of trisodium phosphate, 0.25-0.75 part of sodium silicate, 5.25 parts of sodium sulfate, 0.1-0.3 part of carboxyl methyl cellulose and 0.4-1.2 parts of tallow fatty acid sodium sulfate.

2. The cleaning solution and the cleaning method for dendrobium officinale tissue culture seedlings according to claim 1, wherein the cleaning solution comprises the following components: 45 parts of clean water, 0.5 part of sodium alkyl aryl sulfonate, 2 parts of trisodium phosphate, 0.25 part of sodium silicate, 1.75 parts of sodium sulfate, 0.1 part of carboxyl methylcellulose and 0.4 part of tallow fatty acid sodium sulfate;

composition of matter Portion measuring Clean water 45 portions of Alkyl aryl sulfonic acid sodium salt 0.5 portion Trisodium phosphate 2 portions of Sodium silicate 0.25 part Sodium sulfate 1.75 portions Carboxy methylcellulose 0.1 part Sodium tallow fatty acid sulfate 0.4 portion of

。

3. The cleaning solution and the cleaning method for dendrobium officinale tissue culture seedlings according to claim 1, wherein the cleaning solution comprises the following components: 90 parts of clear water, 1 part of sodium alkyl aryl sulfonate, 4 parts of trisodium phosphate, 0.5 part of sodium silicate, 3.5 parts of sodium sulfate, 0.2 part of carboxyl methylcellulose and 0.8 part of tallow fatty acid sodium sulfate;

composition of matter Portion measuring Clean water 90 portions of Alkyl aryl sulfonic acid sodium salt 1 part of Trisodium phosphate 4 portions of Sodium silicate 0.5 portion Sodium sulfate 3.5 parts of Carboxy methylcellulose 0.2 part Sodium tallow fatty acid sulfate 0.8 portion of

。

4. The cleaning solution and the cleaning method for dendrobium officinale tissue culture seedlings according to claim 1, wherein the cleaning solution comprises the following components: 135 parts of clear water, 1.5 parts of sodium alkyl aryl sulfonate, 6 parts of trisodium phosphate, 0.75 part of sodium silicate, 5.25 parts of sodium sulfate, 0.3 part of carboxyl methylcellulose and 1.2 parts of tallow fatty acid sodium sulfate;

composition of matter Portion measuring Clean water 135 portions of Alkyl aryl sulfonic acid sodium salt 1.5 parts of Trisodium phosphate 6 portions of Sodium silicate 0.75 portion Sodium sulfate 5.25 parts of Carboxy methylcellulose 0.3 part Sodium tallow fatty acid sulfate 1.2 parts of

。

5. The cleaning solution and the cleaning method for dendrobium officinale tissue culture seedlings according to claim 1, wherein the dendrobium officinale seedlings are dipped in the cleaning solution and lightly scrubbed when being cleaned, and are fully cleaned by tap water.

6. The cleaning solution and the cleaning method for the dendrobium officinale tissue culture seedlings according to claim 1, wherein the contaminated seedlings and the bare-rooted seedlings or the seedlings with few roots of the dendrobium officinale tissue culture seedlings are separately placed and cleaned, the tissue culture seedlings are firstly scrubbed by the cleaning solution and then cleaned by tap water, and agar is mainly washed off to avoid root rot caused by agar mildew.

Technical Field

The invention relates to the field of cleaning solution for dendrobium officinale tissue culture seedlings, and particularly relates to cleaning solution for dendrobium officinale tissue culture seedlings and a cleaning method thereof.

Background

The Dendrobium officinale seedling is seedling of Dendrobium officinale, also named as herba Hedyotidis Diffusae and Yunnan herba Dendrobii, and belongs to Dendrobium of Orchidaceae. The stem is upright, cylindrical, the leaves are in two rows, the paper is made of paper, the long round is in a needle shape, the general inflorescence usually comes out from the upper part of the old stem with the fallen leaves, 2-3 flowers are provided, sepals and petals are yellow green and are similar, the long round is in a needle shape, and the flowering period is 3-6 months. The dendrobium officinale is suitable for growing in a cool, humid and air-free environment and is usually grown on semi-shady and humid rocks in mountainous regions with the altitude of 1600 meters. The dendrobium officinale is a traditional rare Chinese medicinal material in China, and has the effects of nourishing yin, clearing heat, benefiting stomach, promoting fluid production and the like. In addition, the dendrobium officinale is an orchid plant, is strong in vitality and perennial, is a brand-new flower, can bloom in 5-6 months every year, is aromatic in flavor, is light yellow green, grass yellow or light yellow, and has high appreciation value and economic value.

In the prior art, the cultivation and cleaning of the dendrobium officinale tissue culture seedlings are usually performed by adopting flowing clear water, so that agar adhered to roots of the dendrobium officinale tissue culture seedlings is difficult to quickly clean in the cleaning process, the cleaning time is too long, the cleaning efficiency of the dendrobium officinale tissue culture seedlings is reduced, and the survival rate of the dendrobium officinale tissue culture seedlings is reduced, and therefore a cleaning solution and a cleaning method for the dendrobium officinale tissue culture seedlings are needed.

Disclosure of Invention

The invention aims to provide a cleaning solution for dendrobium officinale tissue culture seedlings and a cleaning method thereof, and aims to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a cleaning solution for dendrobium officinale tissue culture seedlings and a cleaning method thereof are disclosed, wherein the cleaning solution comprises the following components in parts by weight: 45-135 parts of clean water, 0.5-1.5 parts of alkyl aryl sodium sulfonate, 2-6 parts of trisodium phosphate, 0.25-0.75 part of sodium silicate, 5.25 parts of sodium sulfate, 0.1-0.3 part of carboxyl methyl cellulose and 0.4-1.2 parts of tallow fatty acid sodium sulfate.

Preferably, the cleaning solution comprises the following components: 45 parts of clean water, 0.5 part of sodium alkyl aryl sulfonate, 2 parts of trisodium phosphate, 0.25 part of sodium silicate, 1.75 parts of sodium sulfate, 0.1 part of carboxyl methylcellulose and 0.4 part of tallow fatty acid sodium sulfate.

Preferably, the cleaning solution comprises the following components: 90 parts of clear water, 1 part of sodium alkyl aryl sulfonate, 4 parts of trisodium phosphate, 0.5 part of sodium silicate, 3.5 parts of sodium sulfate, 0.2 part of carboxyl methylcellulose and 0.8 part of tallow fatty acid sodium sulfate.

Preferably, the cleaning solution comprises the following components: 135 parts of clear water, 1.5 parts of sodium alkyl aryl sulfonate, 6 parts of trisodium phosphate, 0.75 part of sodium silicate, 5.25 parts of sodium sulfate, 0.3 part of carboxyl methylcellulose and 1.2 parts of tallow fatty acid sodium sulfate.

Preferably, when the dendrobium officinale seedlings are cleaned, the dendrobium officinale seedlings are dipped in cleaning liquid and lightly scrubbed, and are fully cleaned by tap water.

Preferably, the polluted seedlings and the bare-rooted seedlings or the few-rooted seedlings of the dendrobium officinale tissue culture seedlings are separately placed and cleaned, the tissue culture seedlings are firstly scrubbed by cleaning solution and then cleaned by tap water, and agar is mainly cleaned off so as to avoid root rot caused by agar mildewing.

The invention has the technical effects and advantages that:

washing young stem segments with the length of 2-5cm cut from dendrobium officinale plants under running water; dipping a little of cleaning solution by a brush for slight brushing, selecting 90 parts of clear water, 1 part of alkyl aryl sodium sulfonate, 4 parts of trisodium phosphate, 0.5 part of sodium silicate, 3.5 parts of sodium sulfate, 0.2 part of carboxyl methyl cellulose and 0.8 part of tallow fatty acid sodium sulfate by weight parts of the cleaning solution, and fully washing by tap water after the brushing is finished; sterilizing the cleaned stem segments in 75% alcohol for 30 s; the stem segments after disinfection are placed in 0.1% of HgCIz water solution to be soaked for 8-10 min, and finally, the stem segments are washed by sterile water for 5-6 times, so that the cleaning of the dendrobium officinale tissue culture seedlings is more convenient, the problem that agar is not clean due to incomplete cleaning of the dendrobium officinale tissue culture seedlings is solved, the root rot probability caused by agar mildew is reduced, the cleaning efficiency is improved, the damage to the dendrobium officinale tissue culture seedlings due to long cleaning time is avoided, and the cleaning efficiency of the dendrobium officinale tissue culture seedlings is improved.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a cleaning solution for dendrobium officinale tissue culture seedlings and a cleaning method thereof, wherein the cleaning solution comprises the following components in parts by weight: 45-135 parts of clean water, 0.5-1.5 parts of alkyl aryl sodium sulfonate, 2-6 parts of trisodium phosphate, 0.25-0.75 part of sodium silicate, 5.25 parts of sodium sulfate, 0.1-0.3 part of carboxyl methyl cellulose and 0.4-1.2 parts of tallow fatty acid sodium sulfate.

The cleaning solution comprises the following components: 45 parts of clean water, 0.5 part of sodium alkyl aryl sulfonate, 2 parts of trisodium phosphate, 0.25 part of sodium silicate, 1.75 parts of sodium sulfate, 0.1 part of carboxyl methylcellulose and 0.4 part of tallow fatty acid sodium sulfate;

composition of matter	Portion measuring
		Clean water	45 portions of
Alkyl aryl sulfonic acid sodium salt	0.5 portion
		Trisodium phosphate	2 portions of
Sodium silicate	0.25 part
		Sodium sulfate	1.75 portions
Carboxy methylcellulose	0.1 part
		Sodium tallow fatty acid sulfate	0.4 portion of

The cleaning solution comprises the following components: 90 parts of clear water, 1 part of sodium alkyl aryl sulfonate, 4 parts of trisodium phosphate, 0.5 part of sodium silicate, 3.5 parts of sodium sulfate, 0.2 part of carboxyl methylcellulose and 0.8 part of tallow fatty acid sodium sulfate;

composition of matter	Portion measuring
		Clean water	90 portions of
Alkyl aryl sulfonic acid sodium salt	1 part of
		Trisodium phosphate	4 portions of
Sodium silicate	0.5 portion
		Sodium sulfate	3.5 parts of
Carboxy methylcellulose	0.2 part
		Sodium tallow fatty acid sulfate	0.8 portion of

The cleaning solution comprises the following components: 135 parts of clear water, 1.5 parts of sodium alkyl aryl sulfonate, 6 parts of trisodium phosphate, 0.75 part of sodium silicate, 5.25 parts of sodium sulfate, 0.3 part of carboxyl methylcellulose and 1.2 parts of tallow fatty acid sodium sulfate;

composition of matter	Portion measuring
		Clean water	135 portions of
Alkyl aryl sulfonic acid sodium salt	1.5 parts of
		Trisodium phosphate	6 portions of
Sodium silicate	0.75 portion
		Sodium sulfate	5.25 parts of
Carboxy methylcellulose	0.3 part
		Sodium tallow fatty acid sulfate	1.2 parts of

When the dendrobium officinale seedlings are cleaned, the dendrobium officinale seedlings are dipped in cleaning liquid and lightly scrubbed and are fully cleaned by tap water.

The contaminated seedlings and bare-rooted seedlings or few-rooted seedlings of the dendrobium officinale tissue culture seedlings are separately placed and cleaned, the tissue culture seedlings are firstly scrubbed by cleaning solution and then cleaned by tap water, and agar is mainly washed off so as to prevent the agar from mildewing to cause root rot.

A cleaning method of dendrobium officinale tissue culture seedlings comprises the following steps:

firstly, a worker washes young and tender stem sections which are cut off from a dendrobium officinale plant and have the length of 2-5cm under running water;

step two, dipping a little of cleaning solution by using a brush for slight brushing, selecting 90 parts by weight of clear water, 1 part by weight of alkyl aryl sodium sulfonate, 4 parts by weight of trisodium phosphate, 0.5 part by weight of sodium silicate, 3.5 parts by weight of sodium sulfate, 0.2 part by weight of carboxyl methyl cellulose and 0.8 part by weight of tallow fatty acid sodium sulfate, and fully cleaning by using tap water after the brushing is finished;

step three, placing the cleaned stem segments in 75% alcohol for disinfection for 30 s;

and step four, placing the disinfected stem segments in 0.1% HgCIz water solution for soaking for 8-10 min, and finally washing with sterile water for 5-6 times.

The working principle of the invention is as follows: working personnel wash young and tender stem sections which are cut off from dendrobium officinale plants and have the length of 2-5cm under running water; dipping a little of cleaning solution by a brush for slight brushing, selecting 90 parts of clear water, 1 part of alkyl aryl sodium sulfonate, 4 parts of trisodium phosphate, 0.5 part of sodium silicate, 3.5 parts of sodium sulfate, 0.2 part of carboxyl methyl cellulose and 0.8 part of tallow fatty acid sodium sulfate by weight parts of the cleaning solution, and fully washing by tap water after the brushing is finished; sterilizing the cleaned stem segments in 75% alcohol for 30 s; and (3) placing the disinfected stem segments in 0.1% HgCIz water solution to soak for 8-10 min, and finally washing with sterile water for 5-6 times, so that the root rot probability caused by agar mildew is reduced, the cleaning efficiency is improved, the damage to the dendrobium officinale tissue culture seedlings caused by long cleaning time is avoided, and the cleaning efficiency of the dendrobium officinale tissue culture seedlings is improved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.