阅读说明：本技术 一种植物汁液成分在线检测系统及检测方法 (Plant juice component online detection system and detection method ) 是由 陈立平 王成 侯佩臣 罗斌 张晗 康凯 于 2021-08-16 设计创作，主要内容包括：本发明提供一种植物汁液成分在线检测系统及检测方法,所述植物汁液成分在线检测系统包括注液装置、取样装置、样品收集装置及检测装置；取样装置包括驱动机构与取样头,驱动机构与取样头连接,注液装置与样品收集装置分别与取样头连通；驱动机构用于驱动取样头伸入至植物茎秆或果实内；注液装置用于向取样头内注入溶剂；样品收集装置用于收集从取样头流出的植物汁液；检测装置用于检测植物汁液中目标物质的含量或活性。本发明可便捷地实现对植物汁液的在线提取,不仅可避免对植物造成不可逆的损坏或伤害,而且提取效率高,保证了所提取的目标物质的活性,确保了对植物汁液中目标物质的含量或活性检测的准确性。(The invention provides a plant juice component online detection system and a detection method, wherein the plant juice component online detection system comprises a liquid injection device, a sampling device, a sample collection device and a detection device; the sampling device comprises a driving mechanism and a sampling head, the driving mechanism is connected with the sampling head, and the liquid injection device and the sample collection device are respectively communicated with the sampling head; the driving mechanism is used for driving the sampling head to extend into the plant stem or fruit; the liquid injection device is used for injecting a solvent into the sampling head; the sample collecting device is used for collecting plant juice flowing out of the sampling head; the detection device is used for detecting the content or activity of the target substance in the plant juice. The invention can conveniently realize the on-line extraction of the plant juice, not only can avoid irreversible damage or injury to the plant, but also has high extraction efficiency, ensures the activity of the extracted target substance and ensures the accuracy of the detection of the content or the activity of the target substance in the plant juice.)

1. An on-line detection system for plant juice components, comprising: the device comprises a liquid injection device, a sampling device, a sample collecting device and a detection device;

the sampling device comprises a driving mechanism and a sampling head, the driving mechanism is connected with the sampling head, and the liquid injection device and the sample collection device are respectively communicated with the sampling head;

wherein the driving mechanism is used for driving the sampling head to extend into the plant stem or the fruit; the liquid injection device is used for injecting a solvent into the sampling head; the sample collecting device is used for collecting plant juice flowing out of the sampling head; the detection device is used for detecting the content or activity of the target substance in the plant juice.

2. The system for on-line detection of plant juice component according to claim 1, wherein said sampling head comprises a housing, a filter screen and a filter membrane;

the shell is cup-shaped; the first end of the shell is an open end and is used for extending into the plant stem or the fruit, and the second end of the shell is opposite to the first end;

the filter screen and the filter membrane are arranged in the shell, the filter screen is close to the first end of the shell, and the filter membrane is arranged between the filter screen and the second end of the shell; a first chamber is formed between the filter screen and the filter membrane, and a second chamber is formed between the filter membrane and the second end of the shell; the liquid injection device and the sample collection device are respectively communicated with the second chamber;

wherein the filter screen and the filter membrane can allow the solvent to pass through; the filter screen is used for filtering solid impurities in the plant juice so that the plant juice can enter the first cavity; the filter membrane is used for allowing target substances in the plant juice within a target molecular weight range to selectively enter the second chamber from the first chamber.

3. The system for on-line detection of plant juice components according to claim 2, wherein the second end of the housing has a liquid inlet and a liquid outlet; the liquid inlet is communicated with the liquid injection device, and the liquid outlet is communicated with the sample collection device;

and/or a knife edge extending along the circumferential direction is arranged at the open end of the shell sleeve, and the plane where the open end of the shell sleeve is located is vertical to the central axis of the shell sleeve.

4. The system for on-line measuring juice contents of plants according to claim 2, wherein said driving mechanism comprises a main body column, a rotating assembly and a clamping assembly;

one end of the clamping component is connected with the main body column, and the other end of the clamping component and the first end of the main body column are used for clamping the opposite sides of the plant stalks or fruits;

the rotating assembly comprises a rotating handle and a connecting rod; the main body column, the rotating handle and the connecting rod are coaxially arranged; one end of the connecting rod extends into the axial through hole in the main body column and is detachably connected with one end of the sampling head, and the other end of the connecting rod is connected with the rotating handle; the rotating handle is rotatably arranged at the second end of the main body column and can move along the central axis of the main body column.

5. The system for on-line detection of plant juice components according to claim 4, wherein the side surface of the rotating handle is provided with first scale lines arranged along the circumferential direction thereof; and the side surface of the main body column is provided with second scale marks which are arranged along the axial direction of the main body column.

6. The on-line plant juice ingredient detection system according to claim 4, wherein said clamp assembly comprises a clamp plate and a support arm;

the supporting arm is arc-shaped and is used for encircling the surface of the plant stalk or the fruit; one end of the supporting arm is connected with the main body column, and the other end of the supporting arm is connected with the clamping plate; the clamp plate and the first end of the main body column are used for clamping opposite sides of the plant stem or the fruit.

7. The system for on-line detection of plant juice components according to claim 4, wherein the liquid injection device comprises a liquid inlet pipe and a liquid injection pump; the liquid inlet end of the liquid injection pump is communicated with the solvent storage device, the liquid outlet end of the liquid injection pump is communicated with one end of the liquid inlet pipe, and the other end of the liquid inlet pipe is communicated with the second chamber;

the sample collecting device comprises a liquid outlet pipe and a collecting bottle; one end of the liquid outlet pipe is communicated with the collecting bottle, and the other end of the liquid outlet pipe is communicated with the second chamber.

8. The on-line plant juice ingredient detection system according to claim 7, wherein said connecting rod is a hollow rod; the liquid inlet pipe and the liquid outlet pipe are both flexible pipes; the other end of the liquid inlet pipe and the other end of the liquid outlet pipe both extend into the connecting rod and are communicated with the second cavity in the sampling head.

9. The system for on-line detection of plant juice component according to claim 7, wherein said liquid injection pump is provided with a flow rate adjusting device;

under the condition that the collecting bottle is horizontally arranged, the caliber of the inner cavity of the collecting bottle is gradually increased from bottom to top; the detection device is arranged in the collecting bottle, and the detection end of the detection device extends into the collecting bottle.

10. A method for detecting a plant juice component in-line detecting system as claimed in any one of claims 1 to 9, comprising:

determining the type of a filtering membrane and the type of a solvent filled into the plant stalks or fruits according to the sampling depth of plant juice in the plant stalks or fruits;

starting the driving mechanism, and controlling the driving mechanism to drive the sampling head to extend into a target position in the plant stem or fruit according to the sampling depth;

starting the liquid injection device, injecting the selected solvent to the sampling head at a preset pressure so as to drive the plant juice at the target position to flow to the sample collection device after passing through the selected filtering membrane, wherein the filtering membrane is used for allowing target substances in the plant juice within a target molecular weight range to selectively permeate;

and detecting the content or activity of the target substance in the plant juice collected by the sample collection device by using a detection device.

Technical Field

The invention relates to the technical field of agriculture, in particular to an online detection system and method for plant juice components.

Background

Plant juice contains many bioactive substances such as water, mineral elements, sugar, amino acids, hormones, etc. Before the component of the plant juice is detected, the plant juice is extracted.

In the related art, when plant juice is obtained, a plant stem or fruit sample is obtained mostly by picking, cutting, shearing, harvesting and the like, and then the plant juice is obtained through pretreatment. Irreversible damage or injury to plants, especially to valuable plants, can occur throughout the treatment process. Meanwhile, complicated pretreatment requires procedures such as drying, grinding, digestion, dissolution, dilution and the like, the above-mentioned cyclic joints cause loss, activity loss, oxidative denaturation and the like of the substance to be detected, and the final detection result cannot truly represent the content or activity of the substance to be detected.

Disclosure of Invention

The invention provides a plant juice component online detection system and a detection method, which are used for solving the problems that the current detection of plant juice components is easy to damage plants and the accuracy of detection results is difficult to ensure.

The invention provides an on-line detection system for plant juice components, which comprises: the device comprises a liquid injection device, a sampling device, a sample collecting device and a detection device; the sampling device comprises a driving mechanism and a sampling head, the driving mechanism is connected with the sampling head, and the liquid injection device and the sample collection device are respectively communicated with the sampling head; wherein the driving mechanism is used for driving the sampling head to extend into the plant stem or the fruit; the liquid injection device is used for injecting a solvent into the sampling head; the sample collecting device is used for collecting plant juice flowing out of the sampling head; the detection device is used for detecting the content or activity of the target substance in the plant juice.

According to the plant juice component online detection system provided by the invention, the sampling head comprises a shell sleeve, a filter screen and a filter membrane; the shell is cup-shaped; the first end of the shell is an open end and is used for extending into the plant stem or the fruit, and the second end of the shell is opposite to the first end; the filter screen and the filter membrane are arranged in the shell, the filter screen is close to the first end of the shell, and the filter membrane is arranged between the filter screen and the second end of the shell; a first chamber is formed between the filter screen and the filter membrane, and a second chamber is formed between the filter membrane and the second end of the shell; the liquid injection device and the sample collection device are respectively communicated with the second chamber; wherein the filter screen and the filter membrane can allow the solvent to pass through; the filter screen is used for filtering solid impurities in the plant juice so that the plant juice can enter the first cavity; the filter membrane is used for allowing target substances in the plant juice within a target molecular weight range to selectively enter the second chamber from the first chamber.

According to the plant juice component online detection system provided by the invention, the second end of the shell sleeve is provided with a liquid inlet and a liquid outlet; the liquid inlet is communicated with the liquid injection device, and the liquid outlet is communicated with the sample collection device; and/or a knife edge extending along the circumferential direction is arranged at the open end of the shell sleeve, and the plane where the open end of the shell sleeve is located is vertical to the central axis of the shell sleeve.

According to the plant juice component online detection system provided by the invention, the driving mechanism comprises a main body column, a rotating assembly and a clamping assembly; one end of the clamping component is connected with the main body column, and the other end of the clamping component and the first end of the main body column are used for clamping the opposite sides of the plant stalks or fruits; the rotating assembly comprises a rotating handle and a connecting rod; the main body column, the rotating handle and the connecting rod are coaxially arranged; one end of the connecting rod extends into the axial through hole in the main body column and is detachably connected with one end of the sampling head, and the other end of the connecting rod is connected with the rotating handle; the rotating handle is rotatably arranged at the second end of the main body column and can move along the central axis of the main body column.

According to the plant juice component online detection system provided by the invention, the side surface of the rotating handle is provided with first scale marks which are distributed along the circumferential direction of the rotating handle; and the side surface of the main body column is provided with second scale marks which are arranged along the axial direction of the main body column.

According to the plant juice component on-line detection system provided by the invention, the clamping assembly comprises a clamping plate and a supporting arm; the supporting arm is arc-shaped and is used for encircling the surface of the plant stalk or the fruit; one end of the supporting arm is connected with the main body column, and the other end of the supporting arm is connected with the clamping plate; the clamp plate and the first end of the main body column are used for clamping opposite sides of the plant stem or the fruit.

According to the plant juice component online detection system provided by the invention, the liquid injection device comprises a liquid inlet pipe and a liquid injection pump; the liquid inlet end of the liquid injection pump is communicated with the solvent storage device, the liquid outlet end of the liquid injection pump is communicated with one end of the liquid inlet pipe, and the other end of the liquid inlet pipe is communicated with the second chamber; the sample collecting device comprises a liquid outlet pipe and a collecting bottle; one end of the liquid outlet pipe is communicated with the collecting bottle, and the other end of the liquid outlet pipe is communicated with the second chamber.

According to the plant juice component online detection system provided by the invention, the connecting rod is a hollow rod; the liquid inlet pipe and the liquid outlet pipe are both flexible pipes; the other end of the liquid inlet pipe and the other end of the liquid outlet pipe both extend into the connecting rod and are communicated with the second cavity in the sampling head.

According to the plant juice component online detection system provided by the invention, the liquid injection pump is provided with a flow regulating device; under the condition that the collecting bottle is horizontally arranged, the caliber of the inner cavity of the collecting bottle is gradually increased from bottom to top; the detection device is arranged in the collecting bottle, and the detection end of the detection device extends into the collecting bottle.

The invention also provides a detection method of the plant juice component online detection system, which comprises the following steps: determining the type of a filtering membrane and the type of a solvent filled into the plant stalks or fruits according to the sampling depth of plant juice in the plant stalks or fruits; starting the driving mechanism, and controlling the driving mechanism to drive the sampling head to extend into a target position in the plant stem or fruit according to the sampling depth; starting the liquid injection device, injecting the selected solvent to the sampling head at a preset pressure so as to drive the plant juice at the target position to flow to the sample collection device after passing through the selected filtering membrane, wherein the filtering membrane is used for allowing target substances in the plant juice within a target molecular weight range to selectively permeate; and detecting the content or activity of the target substance in the plant juice collected by the sample collection device by using a detection device.

According to the plant juice component online detection system and the detection method provided by the invention, by arranging the liquid injection device, the sampling device, the sample collection device and the detection device, the sampling head can be extended into the plant stem or fruit through the sampling device, and then the solvent is injected into the sampling head through the liquid injection device, so that the target substance dynamically synthesized or transported in the plant stem or fruit is carried out by the solvent and collected in the sample collection device, and the detection device can detect the content or activity of the target substance in the plant juice collected by the sample collection device.

Therefore, the method can conveniently realize the on-line extraction of the plant juice, avoids the pretreatment process and the complex detection procedure existing in the existing plant juice extraction, can avoid irreversible damage or injury to the plant, has high extraction efficiency, ensures the activity of the extracted target substance, and ensures the accuracy of the detection of the content or the activity of the target substance in the plant juice.

Drawings

In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and those skilled in the art can also obtain other drawings according to the drawings without creative efforts.

FIG. 1 is a schematic structural diagram of an on-line detection system for plant juice components provided by the present invention;

FIG. 2 is a schematic cross-sectional view of a sampling head according to the present invention;

FIG. 3 is a schematic flow chart of the detection method based on the plant juice component on-line detection system provided by the invention;

reference numerals:

1: a liquid injection device; 2: a sampling device; 3: a sample collection device;

4: a detection device; 11: a liquid injection pump; 12: a liquid inlet pipe;

21: a drive mechanism; 22: a sampling head; 210: a main body column;

211: rotating the handle; 212: a connecting rod; 213: a splint;

214: a support arm; 201: a first scale mark; 202: a second scale mark;

220: a shell; 221: a filter screen; 222: a filtration membrane;

223: a first chamber; 224: a second chamber; 225: a liquid inlet;

226: a liquid outlet; 227: a knife edge; 31: a collection bottle;

32: a liquid outlet pipe.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The following describes a plant juice component on-line detection system and a detection method of the invention with reference to fig. 1-3.

As shown in fig. 1, the present embodiment provides an on-line detection system for plant juice components, comprising: the device comprises a liquid injection device 1, a sampling device 2, a sample collection device 3 and a detection device 4; the sampling device 2 comprises a driving mechanism 21 and a sampling head 22, the driving mechanism 21 is connected with the sampling head 22, and the liquid injection device 1 and the sample collection device 3 are respectively communicated with the sampling head 22; wherein, the driving mechanism 21 is used for driving the sampling head 22 to extend into the plant stem or fruit; the liquid injection device 1 is used for injecting a solvent into the sampling head 22; the sample collection device 3 is used for collecting plant juice flowing out of the sampling head 22; the detection device 4 is used for detecting the content or activity of the target substance in the plant juice.

Specifically, the detection system shown in this embodiment is provided with the liquid injection device 1, the sampling device 2, the sample collection device 3 and the detection device 4, the sampling head 22 can be extended into the plant stem or the fruit through the sampling device 2, and then the solvent is injected into the sampling head 22 through the liquid injection device 1, so that the target substance dynamically synthesized or transported in the plant stem or the fruit is carried out by the solvent, and is collected in the sample collection device 3, so that the detection device 4 detects the content or activity of the target substance in the plant juice collected by the sample collection device 3.

Therefore, the detection system shown in the embodiment can conveniently realize the on-line extraction of the plant juice, avoids the pretreatment process and the complex detection procedure existing in the existing plant juice extraction, can avoid irreversible damage or injury to the plant, has high extraction efficiency, ensures the activity of the extracted target substance, and ensures the accuracy of the content or activity detection of the target substance in the plant juice.

It should be noted that, in the case of extracting plant juice, because there is a difference in the components of different tissue parts in the plant stem or fruit, for example, the target substance of the phloem of the plant stem is mainly based on the organic matter component, and the target substance of the xylem is mainly based on the moisture and the inorganic ion component, so that the present embodiment can determine the type of the solvent according to the sampling depth of the sampling head 22. Obviously, in the case where the sampling head 22 extends into the phloem of the plant stem or fruit, the solvent selected in this embodiment is preferably a fat-soluble solvent, and in the case where the sampling head 22 extends into the xylem of the plant stem or fruit, the solvent selected in this embodiment is preferably a water-soluble solvent.

Meanwhile, the driving mechanism 21 shown in the present embodiment may be a telescopic driving mechanism, a rotary driving mechanism, a mechanical impact mechanism, etc. capable of driving the sampling head 22 to extend into different depth positions in the plant stem or fruit, and is not limited in particular. Thus, the present embodiment can ensure sampling of the same site in the plant tissue under the condition that the biological sample is repeated or under the same sampling condition for a plurality of times, so as to ensure the consistency of the plant juice components.

As shown in fig. 2, the sampling head 22 of the present embodiment includes a housing 220, a filter screen 221 and a filter membrane 222; shell 220 is cup-shaped; the first end of the shell 220 is an open end and is used for extending into the plant stem or fruit, and the second end of the shell 220 is opposite to the first end; the filter screen 221 and the filter membrane 222 are arranged in the shell 220, the filter screen 221 is close to the first end of the shell 220, and the filter membrane 222 is arranged between the filter screen 221 and the second end of the shell 220; a first chamber 223 is formed between the filter net 221 and the filter membrane 222, and a second chamber 224 is formed between the filter membrane 222 and the second end of the shell 220; the liquid injection device 1 and the sample collection device 3 are respectively communicated with the second chamber 224; wherein, the filter screen 221 and the filter membrane 222 can be used for solvent to pass through; the filter screen 221 is used for filtering solid impurities in the plant juice to allow the plant juice to enter the first chamber 223; the filter membrane 222 is used for allowing the target substances in the plant juice to selectively pass from the first chamber 223 to the second chamber 224.

Specifically, in this embodiment, based on the optimized design of the sampling head 22, when the liquid injection device 1 injects the solvent into the sampling head 22, the solvent firstly enters the second chamber 224, then sequentially passes through the filtering membrane 222 and the filtering net 221, enters the plant tissue at the corresponding depth of the plant stem or fruit, and is mixed with the organic substance or the inorganic substance dynamically synthesized or transported in the plant tissue to form the mixed plant juice; then, under the action of the injection pressure, the plant juice is filtered by the filter screen 221 to remove solid impurities, and then under the action of the selective permeation of the filter membrane 222, the plant juice composed of the target substance and the solvent in the target molecular weight range in the plant juice passes through the filter membrane 222 and is collected by the sample collection device 3.

It should be noted here that, in the actual detection process, the filter membranes 222 having different molecular weight ranges may be selected depending on the detection object (target substance). Meanwhile, in the present embodiment, the physical filtration of the filter screen 221 and the selective filtration of the filter membrane 222 can effectively remove the relevant interfering substances, thereby ensuring the accuracy of the detection result of the plant juice component.

As shown in fig. 2, a second end of the shell 220 of the embodiment is provided with a liquid inlet 225 and a liquid outlet 226; the liquid inlet 225 is communicated with the liquid injection device 1, and the liquid outlet 226 is communicated with the sample collection device 3.

Meanwhile, in order to conveniently control the sampling depth of the sampling head 22, the open end of the shell 220 shown in this embodiment is provided with a knife edge 227 extending along the circumferential direction, and the plane of the open end of the shell 220 is perpendicular to the central axis of the shell 220.

As shown in fig. 1, in order to facilitate the driving of the sampling head 22 to extend into the stem or fruit of the plant, the driving mechanism 21 of the embodiment includes a main body column 210, a rotating component and a clamping component; one end of the clamping component is connected with the main body column 210, and the other end of the clamping component and the first end of the main body column 210 are used for clamping the opposite sides of the plant stalks or fruits; the rotating assembly comprises a rotating handle 211 and a connecting rod 212; the main body column 210, the rotating handle 211 and the connecting rod 212 are coaxially arranged; one end of the connecting rod 212 extends into the axial through hole in the main body column 210 and is detachably connected with one end of the sampling head 22, and the other end of the connecting rod 212 is connected with the rotating handle 211; the rotating handle 211 is rotatably disposed at the second end of the main body column 210 and can move along the central axis of the main body column 210.

Specifically, in actual operation, the other end of the clamping component and the first end of the main body column 210 are clamped on the opposite sides of the plant stem or the fruit, and then the rotating handle 211 is rotated manually, since the rotating handle 211 moves along the central axis of the main body column 210 when rotating, the connecting rod 212 can be driven to rotate by the rotating handle 211, so that the connecting rod 212 moves relative to the main body column 210. When the connecting rod 212 drives the sampling head 22 to extend out from the first end of the main body column 210 and to a predetermined position in the plant stem or fruit, the rotation of the rotating handle 211 is stopped.

Further, in order to accurately control the displacement of the sampling head 22 toward the plant stem or fruit, the present embodiment is provided with a first graduation mark 201 arranged along the circumferential direction of the rotation handle 211 on the side thereof, and a second graduation mark 202 arranged along the axial direction of the main body column 210 on the side thereof. It will be appreciated that the displacement of the sampling head 22 towards the plant stem or fruit penetration is the sum of the readings of the turning handle 211 on the first and second graduation marks 201 and 202.

Further, in order to ensure that the sampling head 22 stably extends into the stem or fruit of the plant, the clamping assembly shown in this embodiment comprises a clamping plate 213 and a supporting arm 214; the supporting arm 214 is arc-shaped and used for encircling the surface of the plant stem or the fruit; one end of the supporting arm 214 is connected with the main body column 210, and the other end is connected with the clamping plate 213; the clamp plate 213 and the first end of the main body column 210 serve to clamp opposite sides of a plant stalk or fruit. In this embodiment, the clamping plate 213 and the main body column 210 may be coplanar, and the supporting arm 214 is an elastic arm, so as to be suitable for reliably fixing and clamping plant stalks or fruits with different diameters.

As shown in fig. 1, the liquid injection device 1 of the present embodiment includes a liquid inlet pipe 12 and a liquid injection pump 11; the liquid inlet end of the liquid injection pump 11 is communicated with the solvent storage device, the liquid outlet end of the liquid injection pump 11 is communicated with one end of the liquid inlet pipe 12, and the other end of the liquid inlet pipe 12 is communicated with the second chamber 224; the sample collection device 3 comprises a liquid outlet pipe 32 and a collection bottle 31; the outlet tube 32 has one end in communication with the collection vial 31 and another end in communication with the second chamber 224.

Further, based on the configuration of the driving mechanism 21 in the present embodiment, in order to ensure that the liquid pump 11 reliably fills the second chamber 224 through the liquid inlet pipe 12 and the second chamber 224 can be smoothly recovered to the collecting bottle 31 through the liquid outlet pipe 32, the connecting rod 212 in the present embodiment is preferably a hollow rod, and the liquid inlet pipe 12 and the liquid outlet pipe 32 are hoses; the other end of inlet tube 12 and the other end of outlet tube 32 both extend into connecting rod 212 and communicate with a second chamber 224 in sampling head 22. Thus, when the connecting rod 212 rotates along with the rotating handle 211, the infusion of the inlet tube 12 and the outlet tube 32 is not affected.

Further, in order to accurately control the injection speed and the injection volume, the present embodiment is provided with a flow rate adjusting device at the injection pump 11, and the flow rate adjusting device may be an adjusting knob capable of adjusting the flow rate of the injection pump 11 as known in the present embodiment.

Meanwhile, in order to facilitate the detection of the collected plant juice, under the condition that the collecting bottle 31 is horizontally arranged, the caliber of the inner cavity of the collecting bottle 31 is gradually increased from bottom to top; the detection device 4 is arranged in the collection bottle 31, and the detection end of the detection device 4 extends into the collection bottle 31.

Wherein, this embodiment can set up the fixed sleeve at the bottleneck of receiving flask 31, and the fixed sleeve is used for installing detection device 4 to ensure that detection device 4's detection end stretches into in receiving flask 31, thereby carry out on-line measuring to the target substance in the plant juice.

As shown in fig. 3, the present embodiment further provides a detection method of the online detection system for plant juice components, which includes:

in step 310, the type of the filtering membrane and the type of the solvent filled into the plant stalks or fruits are determined according to the sampling depth of the plant juice in the plant stalks or fruits.

And step 320, starting the driving mechanism, and controlling the driving mechanism to drive the sampling head to extend into a target position in the plant stem or the fruit according to the sampling depth.

And 330, starting the liquid injection device, injecting the selected solvent to the sampling head at a preset pressure so as to drive the plant juice at the target position to flow to the sample collection device after passing through the selected filtering membrane, wherein the filtering membrane is used for allowing the target substances in the plant juice within the target molecular weight range to selectively permeate.

And 340, detecting the content or activity of the target substance in the plant juice collected by the sample collection device by using a detection device.

Specifically, in the actual detection, after the type of the filtering membrane and the type of the solvent to be filled are determined according to the sampling depth, the sampling head can be extended into the target position in the plant stem or the fruit through the sampling device, and then the solvent of the corresponding type is injected into the sampling head through the liquid injection device, so that the target substance dynamically synthesized or transported in the plant stem or the fruit within the preset molecular weight range is carried out by the solvent under the selective permeation action of the filtering membrane, and is collected in the sample collection device, so that the detection device detects the content or activity of the target substance in the plant juice collected by the sample collection device.

Therefore, the detection method disclosed by the embodiment can be used for conveniently realizing the on-line extraction of the plant juice, avoids the pretreatment process and the complex detection procedure existing in the existing plant juice extraction, can avoid irreversible damage or injury to the plant, is high in extraction efficiency, ensures the activity of the extracted target substance, and ensures the accuracy of the detection of the content or the activity of the target substance in the plant juice. Meanwhile, the embodiment can obtain target substances with different molecular weight ranges based on the type selection of the filtering membrane so as to detect and analyze the content or activity of the target substances in a targeted manner.

The present invention will be described in detail below with reference to the following examples of the extraction and detection of abscisic acid from tomatoes.

Abscisic Acid (S-ABA or ABA) is one of five internationally recognized endogenous plant growth regulating substances with important physiological activities, and is called as the king of plant stress resistance inducing substances. ABA is involved in biological processes such as signal transduction, drought tolerance and the like and is an important hormone of plants. The conventional extraction of ABA currently comprises processes of weighing, grinding, dissolving and the like, and ABA is very easy to degrade or partially lost during the extraction process.

Based on the detection system, the content of ABA of tomato stalks cultured for 30 days in a 20% polyethylene glycol (PEG) solution is detected in real time before and after the simulated drought, and the operation is as follows:

after with tomato stem centre gripping between the relative terminal surface of splint and main part post, through rotatory turning handle to under the cooperation of first scale mark and second scale mark, insert the sample head 5mm degree of depth in the tomato stem, and avoid leaking the solution with the linking department of silica gel seal sample head and connecting rod. Selecting water as a filling solvent, adjusting a flow regulating device when a liquid injection pump is started, and controlling the filled aqueous solution to be input to a sampling head at the speed of 10 mul/min; ABA enters a filtering membrane after being primarily filtered by a filtering net, under the selective permeation action of the filtering membrane, substances with the molecular weight of 10KD can pass through the filtering membrane, the ABA is taken away by aqueous solution and enters a collecting bottle through a liquid outlet pipe, and the water is collected for 10 min; at the moment, a detection device arranged in the collection bottle detects the ABA content in the collection bottle on line, and the ABA content range from 0.10 to 1.31 mu g/g fresh weight under the normal water supply condition to the drought is measured.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.