阅读说明：本技术 一种具有抗应激作用的组合物及其制备方法 (Composition with anti-stress effect and preparation method thereof ) 是由 王强 倪同艳 苗晋锋 张永强 张敬峰 谢长文 刘芳 杨玉姣 罗祖强 陈丽萍 高澜 于 2021-07-30 设计创作，主要内容包括：本发明属于饲料及饲料添加剂技术领域,尤其是一种具有抗应激作用的组合物及其制备方法；其质量份组成如下：硅酸钠200-500份,山梨酸钾3-8份,碳酸钠50-300份,柠檬酸钾100-500份,苯甲酸钠5-10份,四硼酸钠10-15份,硫代硫酸钠5-15份,越橘粉200-550份,茴香粉20-50份,低聚木糖200-1500份,葡萄糖100-500份、蔗糖100-300份,水500-1500份；本发明通过矿物离子和中药提取物的组合,可以显著改善中药提取物的消化吸收,促进机体对营养物质的消化吸收,修复肠道粘膜屏障,减少肠道炎症,减少畜禽因应激而造成的采食量下降和腹泻等症状。(The invention belongs to the technical field of feed and feed additives, in particular to a composition with anti-stress effect and a preparation method thereof; the composition comprises the following components in parts by mass: 500 parts of sodium silicate 200-; the invention can obviously improve the digestion and absorption of the traditional Chinese medicine extract, promote the digestion and absorption of organisms on nutrient substances, repair intestinal mucosa barriers, reduce intestinal inflammation, and reduce the symptoms of feed intake reduction, diarrhea and the like of livestock and poultry caused by stress by combining the mineral ions and the traditional Chinese medicine extract.)

1. A composition with anti-stress effect is characterized in that: the composition comprises the following components in parts by mass: 500 parts of sodium silicate 200-containing material, 3-8 parts of potassium sorbate, 50-300 parts of sodium carbonate, 500 parts of potassium citrate 100-containing material, 5-10 parts of sodium benzoate, 10-15 parts of sodium tetraborate, 5-15 parts of sodium thiosulfate, 550 parts of orange powder 200-containing material, 20-50 parts of fennel powder, 1500 parts of xylo-oligosaccharide 200-containing material, 500 parts of glucose 100-containing material and 300 parts of sucrose 100-containing material.

2. The composition with anti-stress effect according to claim 1, wherein: the composition comprises the following components in parts by mass: 250 parts of sodium silicate, 4 parts of potassium sorbate, 75 parts of sodium carbonate, 200 parts of potassium citrate, 6 parts of sodium benzoate, 12.6 parts of sodium tetraborate, 7.9 parts of sodium thiosulfate, 220 parts of cowberry fruit powder, 25 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of sucrose.

3. The composition with anti-stress effect according to claim 1, wherein: the composition comprises the following components in parts by mass: 450 parts of sodium silicate, 7 parts of potassium sorbate, 265 parts of sodium carbonate, 460 parts of potassium citrate, 8 parts of sodium benzoate, 13 parts of sodium tetraborate, 11.9 parts of sodium thiosulfate, 455 parts of orange powder, 40 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of sucrose.

4. The composition with anti-stress effect according to claim 1, wherein: the composition comprises the following components in parts by mass: 350 parts of sodium silicate, 5 parts of potassium sorbate, 150 parts of sodium carbonate, 357 parts of potassium citrate, 8 parts of sodium benzoate, 12.6 parts of sodium tetraborate, 7.9 parts of sodium thiosulfate, 290 parts of orange powder, 30 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of sucrose.

5. The composition with anti-stress effect according to claim 1, wherein: the Vaccinium myrtillus powder is obtained by pulverizing leaves and fruits of Vaccinium myrtillus of Ericaceae into powder of 80-100 mesh.

6. The composition with anti-stress effect according to claim 1, wherein: the fennel powder is prepared by mixing and crushing anise and fennel in a ratio of 1:1, and the crushing fineness is 80-100 meshes.

7. A preparation method of a composition with anti-stress effect is characterized in that: the preparation method comprises the following steps:

weighing 500 parts of 200-portion sodium silicate ion-containing materials, 3-8 parts of potassium sorbate, 50-300 parts of sodium carbonate, 500 parts of 100-portion potassium citrate ion-containing materials, 5-10 parts of sodium benzoate, 10-15 parts of sodium tetraborate, 5-15 parts of sodium thiosulfate, 10-30 parts of orange powder, 20-50 parts of fennel powder, 1500 parts of 200-portion xylo-oligosaccharide ion-containing materials, 500 parts of 100-portion glucose ion-containing materials and 300 parts of 100-portion sucrose ion-containing materials, sequentially dissolving in 1000 parts of water, stirring for 6-12 hours at the temperature of 60-80 ℃, standing for 48 hours at normal temperature, and filtering to remove impurities to obtain the liquid composition.

Technical Field

The invention belongs to the technical field of feed and feed additives, and particularly relates to a composition with an anti-stress effect and a preparation method thereof.

Background

At present, the livestock and poultry breeding continuously presents large-scale breeding, the modern breeding technical level is continuously improved, but the influence of stress factors on the livestock and poultry is more and more prominent, and the stress response becomes one of the problems that the breeders feel headache greatly in the livestock and poultry breeding process.

All links in the livestock and poultry breeding process have stressors which can be divided into environmental stress: extreme temperature and humidity, strong radiation, airflow (bad ventilation or harmful wind and the like), poor air quality (high concentration of toxic and harmful gases such as ammonia gas, hydrogen sulfide, carbon dioxide and the like, dust), strong noise, insufficient or excessive illumination and the like; feeding management factors: one is stress caused by normal management measures, such as beak breaking, toe breaking, crown cutting, tail breaking, ear number striking, castration, weighing, group transferring, transportation and the like, and the other is stress caused by improper management, such as excessive density, insufficient movement, driving, rough arrest, whipping, management system, personnel replacement and the like; nutritional stressors: hunger or satiety, water shortage, daily ration mutation, feeding mode mutation, daily ration nutrient imbalance, feed poisoning, drug poisoning; biological stressors: such as bacterial, viral, parasitic infestations, and the like; psychological stress: such as animal fighting, frightening, etc.; other factors: such as super-strength breeding and the like.

If the stress intensity is too high or the stress time is too long, the stress load can be caused, the health of animals can be damaged, even pathological damage can be caused, and the death of livestock and poultry can be caused, and the stress is called as bad stress. In livestock and poultry production, people generally pay attention to the harm brought by the inferior stress to livestock and poultry breeding, the excessive stress not only harms the normal growth and development and the production performance of livestock and poultry, but also more importantly influences the immune system of livestock and poultry, so that the organism generates immune suppression to cause various diseases, and the serious harm is brought to the development of the livestock and poultry industry along with higher death rate.

Disclosure of Invention

One object of the present invention is: overcomes the defects in the prior art and provides a composition with anti-stress effect.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the composition with the anti-stress effect comprises the following components in parts by mass: 500 parts of sodium silicate 200-containing material, 3-8 parts of potassium sorbate, 50-300 parts of sodium carbonate, 500 parts of potassium citrate 100-containing material, 5-10 parts of sodium benzoate, 10-15 parts of sodium tetraborate, 5-15 parts of sodium thiosulfate, 550 parts of orange powder 200-containing material, 20-50 parts of fennel powder, 1500 parts of xylo-oligosaccharide 200-containing material, 500 parts of glucose 100-containing material and 300 parts of sucrose 100-containing material.

The further optimized scheme is that the composition comprises the following components in parts by mass: 250 parts of sodium silicate, 4 parts of potassium sorbate, 75 parts of sodium carbonate, 200 parts of potassium citrate, 6 parts of sodium benzoate, 12.6 parts of sodium tetraborate, 7.9 parts of sodium thiosulfate, 220 parts of cowberry fruit powder, 25 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of sucrose.

The further optimized scheme is that the composition comprises the following components in parts by mass: 450 parts of sodium silicate, 7 parts of potassium sorbate, 265 parts of sodium carbonate, 460 parts of potassium citrate, 8 parts of sodium benzoate, 13 parts of sodium tetraborate, 11.9 parts of sodium thiosulfate, 455 parts of orange powder, 40 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of sucrose.

The further optimized scheme is that the composition comprises the following components in parts by mass:

350 parts of sodium silicate, 5 parts of potassium sorbate, 150 parts of sodium carbonate, 357 parts of potassium citrate, 8 parts of sodium benzoate, 12.6 parts of sodium tetraborate, 7.9 parts of sodium thiosulfate, 290 parts of orange powder, 30 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of sucrose.

The further optimized proposal is that the lingonberry powder is powder obtained by crushing leaves and fruits of lingonberry of Ericaceae, and the crushing fineness is 80-100 meshes.

The optimized scheme is that the fennel powder is powder obtained by mixing and crushing anise and fennel in a ratio of 1:1, and the crushing fineness is 80-100 meshes.

Another object of the invention is: overcomes the defects in the prior art, and provides a preparation method of a composition with anti-stress effect, and the preparation method

A method for preparing a composition with anti-stress effect comprises the following steps:

the raw materials are weighed according to any component ratio and gradually dissolved in 1000 parts of water, stirred at the temperature of 60-80 ℃ for 6-12 hours, placed at normal temperature for 48 hours, and filtered to remove impurities to obtain the liquid composition.

The invention has the technical effects and advantages that:

1. the invention can effectively supplement sodium, potassium and other ions which are lacked in the body in the stress process, and maintain the osmotic pressure balance and the acid-base balance of body fluid.

2. According to the invention, the orange powder and the fennel powder are added to provide various anthocyanins, flavonoids, triterpenoids, iridoid compounds, phenolic compounds and the like, so that the oxidation resistance of an organism can be improved, harmful bacteria can be inhibited, the anti-inflammatory effect can be achieved, and the inflammatory reaction of the organism caused by stress can be reduced.

3. The invention can obviously improve the digestion and absorption of the traditional Chinese medicine extract, promote the digestion and absorption of organisms on nutrient substances, repair intestinal mucosa barriers, reduce intestinal inflammation, and reduce the symptoms of feed intake reduction, diarrhea and the like of livestock and poultry caused by stress by combining the mineral ions and the traditional Chinese medicine extract.

Drawings

FIG. 1 Effect of the invention on the amount of MDA in serum;

FIG. 2 Effect of the invention on serum T-AOC;

FIG. 3 Effect of the invention on MDA content in liver;

FIG. 4 Effect of the invention on T-AOC content in liver;

FIG. 5 Effect of the invention on MDA levels in the kidney;

FIG. 6 Effect of the invention on T-AOC levels in kidney.

Detailed Description

In order to better understand the usage of the invention, three specific examples will be presented below, so that the technical solution will be clearly and completely described. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The experimental procedures in the following examples are conventional unless otherwise specified.

Example 1

The invention is realized by the following steps: the composition with the anti-stress effect is prepared from the following components in parts by mass:

250 parts of sodium silicate, 4 parts of potassium sorbate, 75 parts of sodium carbonate, 200 parts of potassium citrate, 6 parts of sodium benzoate, 12.6 parts of sodium tetraborate, 7.9 parts of sodium thiosulfate, 220 parts of cowberry fruit powder, 25 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of cane sugar, which are gradually dissolved in 1000 parts of water, stirred at 60-80 ℃ for 6-12 hours, placed at normal temperature for 48 hours, and filtered to remove impurities to obtain the liquid composition.

Example 2

The invention is realized by the following steps: the composition with the anti-stress effect is prepared from the following components in parts by mass:

450 parts of sodium silicate, 7 parts of potassium sorbate, 265 parts of sodium carbonate, 460 parts of potassium citrate, 8 parts of sodium benzoate, 13 parts of sodium tetraborate, 11.9 parts of sodium thiosulfate, 455 parts of orange powder, 40 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of cane sugar, which are gradually dissolved in 1000 parts of water, stirred at 60-80 ℃ for 6-12 hours, placed at normal temperature for 48 hours, and then filtered to remove impurities to obtain the liquid composition.

Example 3

The invention is realized by the following steps: the composition with the anti-stress effect is prepared from the following components in parts by mass:

350 parts of sodium silicate, 5 parts of potassium sorbate, 150 parts of sodium carbonate, 357 parts of potassium citrate, 8 parts of sodium benzoate, 12.6 parts of sodium tetraborate, 7.9 parts of sodium thiosulfate, 290 parts of orange powder, 30 parts of fennel powder, 800 parts of xylo-oligosaccharide, 300 parts of glucose and 100 parts of cane sugar, which are gradually dissolved in 1000 parts of water, stirred at 60-80 ℃ for 6-12 hours, placed at normal temperature for 48 hours, and filtered to remove impurities to obtain the liquid composition.

Test example 1: influence of different embodiments of the invention on anti-stress effect of laying hens

1.1 design of the experiment

300 Su fowl yellow chickens with the age of 32 weeks, which are clinically healthy, similar in weight and similar in physiological state are selected and randomly divided into 5 groups, each group is 5 in number, and each group is 12 in number. The raising time is selected in summer of 6-9 months, the stress control group and the test group are closed by a henhouse wet curtain cooling device before the test, so that the chickens are exposed to the high-temperature environment of 31.4 +/-1.5 ℃ to generate natural heat stress, and the pre-feeding period is 15 days and the forward-feeding period is 90 days.

The experiment is carried out in a layer chicken farm in Yancheng of Jiangsu, and the traditional immunization, the conventional management, the free intake of food and the drinking of water are carried out. The temperature and humidity change conditions of the henhouse are recorded every day, and the health conditions of the chicken flocks are observed. The basic ration is prepared according to the nutritional requirements of laying hens in the American NRC (1994) poultry nutritional Standard.

TABLE 1 design of the experiments

1.2 measurement indexes and methods

And (4) measuring the production performance, accurately weighing the input amount and the residual amount of the feed by taking the repetition as a unit at 8:00 every day, and recording the egg number. Production performance indices such as average daily food intake, laying rate, and the like were calculated for 1,2, and 3 months (6, 7, and 8 months), respectively.

1.3 test results

TABLE 2 Effect of the invention on Heat stress layer production Performance

Under stress conditions, the average dry matter feed intake and the laying rate of the laying hens are reduced, and as can be seen from table 2, compared with a control group, regarding the average dry matter feed intake, a blank control group > example 2 > example 3 > example 1 > stress control group, the embodiment of the invention can improve the anti-stress capability of the laying hens and improve the feed intake of the laying hens under heat stress conditions. Regarding the laying rate, after the laying hens are subjected to heat stress, the laying rate of each group of laying hens is seriously reduced, and a blank control group > example 2 > example 3 > example 1 > stress control group show that the embodiment of the invention can improve the heat stress resistance of the laying hens and reduce the reduction of the laying rate.

As can be seen from the above tests, the invention can threaten the heat stress resistance of the yellow chicken and has the anti-stress effect of example 2 to example 3 to example 1.

Test example 2: influence of the invention on oxidation resistance of broilers

2.1 design of the experiment

20000 5-day-old Cobb-500 broilers (Cobb-500) which are clinically healthy, have similar body weights and similar physiological states are selected, fed and observed for 3 days before the test, and are randomly divided into 2 groups, namely a control group and a test group (example 2), wherein each group is 6 in number.

The test is carried out in a broiler farm in Jiangsu, and ground flat culture, conventional immunization, conventional management, free feeding and drinking are adopted. Recording the temperature and humidity change conditions of the henhouse every day, observing the health conditions of the chicken flocks, and taking the period from 37 days old to 42 days old as a medicine stopping period. The basic daily ration is prepared according to the nutritional requirements of the broiler chickens in the American NRC (1994) poultry nutritional Standard.

2.2 sample Collection and processing

2.2.1 blood Collection and processing

Collecting blood from front wing vein of chicken at 0, 1,2, 3, 4 weeks after the test, collecting blood about 2mL each time, centrifuging at 3500r/min for 10min, collecting upper layer serum, and storing in refrigerator at-20 deg.C for use, wherein each group contains 6 chickens.

2.2.2 liver and Kidney tissue Collection and processing

Liver and kidney tissues were collected at 0, 1,2, 3, 4 weeks after the start of the experiment, and 6 chickens per group. Adding the sample into normal saline according to a ratio of 1:9(W/V), homogenizing the tissue on ice, centrifuging at 4000r/min and 4 ℃ for 30min, removing the upper layer of grease, and taking the supernatant to store in a refrigerator at-20 ℃ for later use.

2.2.3 immune organ Collection and processing

Weighing on empty stomach on the day of 0, 1,2, 3 and 4 weeks after the test is started, slaughtering spleen, thymus and bursa of fabricius, weighing fresh weight after fat is removed, and weighing 6 chickens in each group.

2.3 detection index and method

Malondialdehyde (MDA), total antioxidant capacity (T-AOC) were analyzed with corresponding kits (all available from Gentronic, Inc.).

2.4 test results

2.4.1 blood index, as shown in figure 1.

FIG. 1 Effect of the invention on the amount of MDA in serum

Malondialdehyde (MDA) is a product of peroxidation of lipid by free radicals in vivo, causes cross-linking polymerization of life macromolecules such as proteins and nucleic acids, and is cytotoxic.

Compared with the control group, the compound has no significant difference, but the overall compound has a decreasing trend. The invention can reduce the MDA concentration in serum to a certain extent and reduce the occurrence of lipid peroxidation.

FIG. 2 is a graph showing the effect of the present invention on serum T-AOC

The antioxidant capacity of the defense system of the organism is closely related to health, the defense system has the functions of decomposing free radicals and active oxygen, decomposing peroxide, removing metal ions with catalytic effect, and measuring the total antioxidant capacity (T-AOC) can reflect the health condition of the organism, and when the total antioxidant capacity of the organism is reduced, inflammation and immune system diseases are easily caused.

As can be seen from FIG. 2, the serum T-AOC was significantly higher in the test groups at 2, 3 and 4 weeks of age than in the control group. The invention is shown to improve the oxidation resistance of the organism to a certain extent.

2.4.2 liver index

FIG. 3 Effect of the invention on the amount of MDA in the liver

MDA was significantly reduced in the liver at 2 and 4 weeks compared to the control group. The invention is shown to reduce the occurrence of lipid peroxidation in the liver.

FIG. 4 Effect of the invention on the T-AOC content in liver

T-AOC was significantly elevated in liver by week 2 compared to control, others were not significantly different, but overall there was an increasing trend. The invention can improve the oxidation resistance of the liver.

2.4.3 Kidney index

FIG. 5 Effect of the invention on MDA levels in kidneys

Compared with the control group, the kidney of the test group has lower MDA content than the control group.

FIG. 6 Effect of the invention on the T-AOC content in the Kidney

Compared with the control group, the compound has no obvious difference, but the overall compound has a rising trend. The invention is shown to improve the oxidation resistance of the organism to a certain extent.

The stress is a common problem in the process of raising livestock and poultry, and causes the stress of the livestock and poultry are various, including overlarge temperature fluctuation, vaccine reaction and the like. The stress has negative effects on the aspects of growth and development of livestock and poultry, including slow growth of the livestock and poultry, anabolic disorder, reduction of feed conversion rate and the like. The oxidation-reduction process is a dynamic oxidation-reduction process in an animal body, when the oxidation-reduction process is damaged, oxidative stress is caused, namely after the animal body is stimulated by different factors (nutrition, harmful substances and the like), a large amount of ROS are generated in the animal body, and the oxidation-reduction process of the animal body is influenced, namely the oxidative stress. In the process of livestock and poultry breeding, oxidative stress influences the quality of meat and reduces the breeding capability.

The tests show that the total antioxidant capacity of blood, liver and kidney of Kebao-500 broiler chicken can be improved, and the MDA content can be reduced, which shows that the antioxidant and anti-stress capacity of livestock and poultry can be obviously improved.

Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.