阅读说明：本技术 一种鉴定母猪发情的方法及其应用 (Method for identifying oestrus of sow and application thereof ) 是由 滕光辉 雷凯栋 宗超 冯飞起 于 2021-05-10 设计创作，主要内容包括：本发明涉及母猪养殖技术领域,具体涉及一种鉴定母猪发情的方法及其应用。本发明的鉴定母猪发情的方法包括：采集待测母猪的尿液,测定所述尿液中雌二醇和促黄体生成素的含量,将所述雌二醇和促黄体生成素的含量分别与雌二醇和促黄体生成素的发情区间阈值进行比较,根据比较结果预测母猪是否发情。该方法针对断奶后母猪进行无应激式、福利化的发情鉴定,节省了人力、降低了生产成本,为母猪的规模化生产提供发情鉴定的技术保障。(The invention relates to the technical field of sow breeding, in particular to a method for identifying oestrus of sows and application thereof. The method for identifying the oestrus of the sow comprises the following steps: collecting urine of a sow to be tested, determining the contents of estradiol and luteinizing hormone in the urine, comparing the contents of estradiol and luteinizing hormone with oestrus interval thresholds of estradiol and luteinizing hormone respectively, and predicting whether the sow oestrus according to a comparison result. The method aims at the stress-free and welfare oestrus identification of the weaned sows, saves labor, reduces production cost and provides technical support for oestrus identification for large-scale production of the sows.)

1. A method of identifying oestrus in sows comprising: collecting urine of a sow to be tested, determining the contents of estradiol and luteinizing hormone in the urine, comparing the contents of estradiol and luteinizing hormone with oestrus interval thresholds of estradiol and luteinizing hormone respectively, and predicting whether the sow oestrus according to a comparison result.

2. The method as claimed in claim 1, wherein the threshold value of estrus interval comprises a threshold value of preliminary estrus interval and a threshold value of significant estrus interval, the threshold value of preliminary estrus interval of estradiol is 110-285 pg/ml, and the threshold value of significant estrus interval is 275-285 pg/ml;

preferably, the primary estrus interval threshold of estradiol is 112-.

3. The method according to claim 1 or 2, wherein the luteinizing hormone has a preliminary estrus interval threshold of 8-12miu/ml and a significant estrus interval threshold of 12-18 miu/ml;

preferably, the luteinizing hormone has a preliminary oestrus interval threshold of 10-11miu/ml and the luteinizing hormone has a significant oestrus interval threshold of 15-16 miu/ml.

4. The method according to any one of claims 1 to 3, wherein the test sow is predicted to be oestrous if the estradiol and luteinizing hormone levels are both below or equal to their respective preliminary oestrus interval thresholds,

and if the content of one of estradiol and luteinizing hormone is higher than the corresponding initial estrus interval threshold value, predicting the estrus of the sow to be tested.

5. The method as claimed in claim 4, wherein if the oestrus of the sow to be tested is predicted, manual back-pressure detection is assisted.

6. The method as claimed in any one of claims 1 to 5, wherein the collecting of urine of the sow to be tested is from the time of the day of weaning of the sow to the time of oestrus.

7. The method of claim 6, wherein during collection, morning urine is collected at 7:00-8:00 am and urine is collected at 14:30-15:30 pm each day;

preferably, the collected urine is precipitated for 2-4 minutes before the contents of estradiol and luteinizing hormone are measured.

8. The method according to any one of claims 1 to 7, wherein the breed of the sow to be tested is a white sow.

9. The method according to any one of claims 1 to 8, wherein the sow to be tested is a sow with 3 to 5 farrowing.

10. Use of the method of any one of claims 1-9 for sow mating and increasing conception rate of sows.

Technical Field

The invention relates to the technical field of sow breeding, in particular to a method for identifying oestrus of sows and application thereof.

Background

At present, the fine breeding of the breeding pig industry becomes a main development trend. The feeding of the breeding pigs, the environmental control and the like have the application of large-scale sensor electronic technology. However, the oestrus identification of sows still mostly adopts the traditional identification method at present and depends on manual operation, including a pudendum external observation method, a boar climbing method, a manual back pressing method and the like. The methods are low in efficiency, have potential biosafety hazards, increase labor force and limit improvement of production benefits. Most importantly, manual emotion check has higher requirements on professional literacy of workers and breeding experience of breeding pigs. The sow is the production core of the pig farm, the reproductive performance of the sow is a key index of the benefit of the pig farm, and the oestrus identification of the sow is an important technical measure for improving the reproductive efficiency and the production benefit of the sow.

The sows begin to estrus after sexual maturity, and the average clear period of the sows is 21 days and lasts for 3-5 days. The oestrus condition of the sows is accurately and scientifically mastered, the mating time is scientifically held, the number of nonproductive days of the sows can be effectively reduced, and unnecessary feed cost expenditure, repeated oestrus check cost of workers and the like can be reduced for a pig farm. In the oestrus period of the sows, the oestrus state of the sows is accurately mastered and the sows are bred, so that the better conception time can be determined, and the method is very important for improving the pregnancy rate of the sows. Therefore, the rapid stress-free estrus identification of the sow plays an important role. When the sow oestrus, the contents of estrogen and luteinizing hormone are obviously improved, and the indexes can be used as test indexes for carrying out oestrus auxiliary detection.

Disclosure of Invention

The invention aims to provide a method for identifying oestrus of sows and also provides application of the method.

Specifically, the invention provides the following technical scheme:

the invention provides a method for identifying oestrus of sows, which comprises the following steps: collecting urine of a sow to be tested, determining the contents of estradiol (E2) and Luteinizing Hormone (LH) in the urine, comparing the contents of estradiol and luteinizing hormone with oestrus interval thresholds of estradiol and luteinizing hormone respectively, and predicting whether the sow oestrous according to the comparison result.

The method comprises the steps of monitoring the contents of estradiol and luteinizing hormone in urine before and after the estrus of a large number of weaned sows every day, fitting a reference curve and the like through MATLAB and origin for the contents of estradiol (E2) and Luteinizing Hormone (LH) in urine collected by each sow every time, constructing a probability model and a hormone early warning value model of sow estrus through data analysis, and finally verifying a detection result through artificial back pressing to determine the interval threshold of estradiol and luteinizing hormone when the sows estrus. Through a large number of practice verifications, the method for predicting the oestrus of the sow by using the interval threshold has high accuracy.

Specifically, the estrus interval threshold comprises a primary estrus interval threshold and a significant estrus interval threshold, wherein the primary estrus interval threshold of estradiol is 110-285 pg/ml, and the significant estrus interval threshold is 275-285 pg/ml. The luteinizing hormone has a primary oestrus interval threshold of 8-12miu/ml and a significant oestrus interval threshold of 12-18 miu/ml.

Preferably, the primary estrus interval threshold of estradiol is 112-.

Preferably, the luteinizing hormone has a preliminary oestrus interval threshold of 10-11miu/ml and a significant oestrus interval threshold of 15-16 miu/ml.

As a preferable scheme of the invention, the threshold value of the significant estrus interval of the estradiol is 280.5pg/ml, and the threshold value of the preliminary estrus interval is 114.07 pg/ml; the significant estrus interval threshold for luteinizing hormone was 15.17miu/ml, and the preliminary estrus interval threshold was 10.17 miu/ml.

According to the method, the specific basis for predicting whether the sow is oestrous according to the comparison result is as follows:

if the contents of the estradiol and the luteinizing hormone are lower than or equal to the corresponding initial estrus interval threshold value, predicting that the sow to be tested is not estrus,

and if the content of one of estradiol and luteinizing hormone is higher than the corresponding initial estrus interval threshold value, predicting the estrus of the sow to be tested.

The method can be combined with manual emotion checking methods such as manual back pressing and the like.

Preferably, if the oestrus of the sow to be detected is predicted, manual back pressing detection is assisted.

In the method, the collection of the urine of the sow to be detected is from the time of the weaning day of the sow to the time of oestrus.

During collection, morning urine was collected at 7:00-8:00 am and 14:30-15:30 pm daily. Morning urine is preferably collected at 7:30 am and 15:00 pm daily.

The urine collection method can reduce the omission rate.

Preferably, the collected urine is precipitated for 2 to 4 minutes before the content measurement is performed.

In the invention, the variety of the sow to be detected is preferably a large white sow. The sow to be detected is preferably a sow farrowing 3-5 times.

The invention also provides application of the method for identifying the oestrus of the sows in sow mating and sow conception rate improvement.

The invention has the beneficial effects that: according to the method for identifying the oestrus of the sow, whether the sow oestrus is oestrous or not is predicted by comparing the contents of estradiol (E2) and Luteinizing Hormone (LH) in the urine of the sow to be detected with the corresponding oestrus interval threshold, and the future oestrus time can be predicted according to the curve trend chart of the change of the contents of estradiol (E2) and Luteinizing Hormone (LH). Tests prove that the accuracy of predicting the oestrus of the sows by using the method can reach 87.5 percent, and the mating success rate of the sows judged to be oestrous by using the method can reach 85.71 percent by mating and continuously tracking.

The invention carries out non-contact, non-stress and welfare oestrus identification on the weaned sows, saves labor, reduces production cost, provides technical support for modern sow production, and provides a feasible technical means for precision livestock industry (PLF).

Drawings

Fig. 1 is a technical route chart of the sow estrus identification method of the present invention.

FIG. 2 is a graph showing the estradiol content of a large white sow during the interrupted estrus cycle of a lactating sow in example 1 of the present invention.

FIG. 3 is a graph of luteinizing hormone content of a large white sow during an interrupted estrus cycle of a lactating sow in accordance with example 1 of the present invention.

FIG. 4 is a graph of the results of normalization of estradiol and luteinizing hormone in example 1 of the present invention.

Detailed Description

The invention provides a method for identifying the oestrus of sows based on urine hormone indexes estradiol and luteinizing hormone, which can predict the oestrus of weaned sows, provide time reference for artificial insemination or natural mating of the sows, realize the prediction of the oestrus of the sows by physiological indexes and determine the optimal mating time for guiding production. The technical route of the method for identifying the oestrus of the sows provided by the invention is shown in figure 1, and the method comprises the following specific steps:

(1) collecting morning urine at 7:00-8:00 am and urine at 14:30-15:30 pm every day from the time of weaning of the sow, precipitating the collected urine for 2-4 min, and measuring the contents of estradiol and luteinizing hormone in the urine;

(2) comparing the hormone content measured in the step (1) with an oestrus interval threshold corresponding to the hormone content, and predicting the oestrus state of the sow according to a comparison result, wherein the primary oestrus interval threshold of estradiol is 110-120pg/ml, the significant oestrus interval threshold of estradiol is 275-285pg/ml, the primary oestrus interval threshold of luteinizing hormone is 8-12miu/ml, and the significant oestrus interval threshold is 12-18 miu/ml;

if the contents of the estradiol and the luteinizing hormone are lower than or equal to the corresponding initial estrus interval threshold value, predicting that the sow to be tested is not estrus,

and if the content of one of estradiol and luteinizing hormone is higher than the corresponding initial estrus interval threshold value, predicting the estrus of the sow to be tested.

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the following examples, the radioimmunoassay kit of Tianjin Jiuding medical bioengineering Limited company was used for radioimmunoassay of all hormone concentrations, and the test instrument was used: GC-2010 type immunity counter, experimental method: radioimmunoassay.

Example 1 determination of hormone content trend Curve and oestrus Interval thresholds

The method comprises the steps of carrying out daily urine collection from weaning to before and after oestrus on 150 weaned large and white sows (random numbers sow-1 to sow-150) from different regions and different farms, taking 50ml of the front urine from the sow on the weaning day (day 1) at 7:30 am every day, collecting 50ml of urine at 15:00 pm, measuring the contents of estradiol and luteinizing hormone in the urine, marking the daily urine date and the sow ear number, confirming the ear mark number of a target sow during urine collection, and registering the measured value of obtained hormone according to the number;

rejecting outliers in the measured values of estradiol and luteinizing hormone and plotting the change in the content of each hormone based on the measured values of estradiol and luteinizing hormone; the hormone content detection data of weaned sows with numbers sow-1 to sow-8 are specifically shown in fig. 2 and 3;

normalizing the measured value data of estradiol and luteinizing hormone by utilizing matlab to obtain a normalized curve; the normalized curves of the weaned sow data with numbers sow-1 to sow-8 are shown in detail in fig. 4;

comparing the curve rule trend of the estradiol and the luteinizing hormone obtained above with a standard reference curve (the curve trend of hormone in blood of a known sow during estrus can be taken as a reference), establishing an estrus probability model through two factor matching, wherein two factors of the estradiol and the luteinizing hormone respectively account for 50% in the weight model to obtain the estrus probability model,

e2 and LH are marked as c1 and c2 respectively, the oestrus probability is Pc1+ Pc2, and the evaluation index of the model is based on a model of the combined action of two hormones.

The artificial back pressing method (when the sow is pressed back, the sow shows that the ears stand still or the body stands still and the back is bowed) is combined with the artificial back pressing method to verify the condition of red and swollen pudendum of the sow, and the oestrus interval threshold values of estradiol and luteinizing hormone are determined as follows: the threshold value of the significant estrus interval of the estradiol is 280.5pg/ml, and the threshold value of the initial estrus interval is 114.07 pg/ml; the significant estrus interval threshold for luteinizing hormone was 15.17miu/ml, and the preliminary estrus interval threshold was 10.17 miu/ml.

Example 2

The embodiment provides a method for identifying oestrus of sows, which comprises the following specific steps:

(1) collecting morning urine at 7:30 am and urine at 15:00 pm every day from the weaning day of the sow, precipitating the collected urine for 3 minutes, and measuring the contents of estradiol and luteinizing hormone in the urine;

(2) comparing the hormone content measured in the step (1) with an estrus interval threshold corresponding to the hormone content, and predicting the estrus state of the sow according to a comparison result, wherein the significant estrus interval threshold of estradiol is 280.5pg/ml, and the initial estrus interval threshold is 114.07 pg/ml; the significant oestrus interval threshold of luteinizing hormone is 15.17miu/ml, and the initial oestrus interval threshold is 10.17 miu/ml;

if the contents of the estradiol and the luteinizing hormone are lower than or equal to the corresponding initial estrus interval threshold value, predicting that the sow to be tested is not estrus,

and if the content of one of estradiol and luteinizing hormone is higher than the corresponding initial estrus interval threshold value, predicting the estrus of the sow to be tested.

Example 3

In the embodiment, the method of the embodiment 2 is used for oestrus prediction of 8 weaned large white multiparous sows (sows with farrowing of 3-5 times), and the artificial back pressing method is combined for result verification. The results are shown in Table 1. Statistics shows that the prediction result of the sow only numbered 6 is inconsistent with the artificial back-pressing detection result, and the accuracy rate of predicting the oestrus of the sow by using the method can reach 87.5%.

TABLE 1 comparison of sow urine hormone content and oestrus verification results

And further breeding the 8 sows, and continuously tracking, wherein the result shows that 1 sow returns estrus and 1 sow does not estrus, and the rest 6 sows enter the gestation period, and the success rate of breeding reaches 85.71%.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.