阅读说明：本技术 一种发酵菌肥及其制备方法和应用 (Fermented bacterial fertilizer and preparation method and application thereof ) 是由 赵升 崔英 毕军来 姜雪连 赵月玲 李孟杰 于 2021-09-14 设计创作，主要内容包括：本发明涉及一种发酵菌肥及其制备方法和应用,属于菌肥技术领域。发酵菌肥的制备方法为：将清水加入到发酵罐中,加热；加入豆粉,蒸煮；再加入清水,液体温度保持在30～40℃；加入红糖、氨基酸、发酵功能菌液和矿源黄腐酸；敞盖发酵；施用前,加入微量元素、发酵功能菌液和硫酸钾和磷酸二氢钾,即得发酵菌肥。本发明成本低廉,制备程序简单,一次制备得到的发酵菌液,能满足规模化种植需要。且添加物中的大豆粉能较为充分和彻底地发酵,具有溶解快、水解完全、无残留等优势,更利于植物吸收或转化,也减少了滴灌管道的结垢和堵塞。(The invention relates to a fermented bacterial fertilizer and a preparation method and application thereof, belonging to the technical field of bacterial fertilizers. The preparation method of the fermentation bacterial fertilizer comprises the following steps: adding clear water into a fermentation tank, and heating; adding bean flour, and steaming; adding clear water, and keeping the temperature of the liquid at 30-40 ℃; adding brown sugar, amino acid, fermentation functional bacteria liquid and mineral fulvic acid; opening the cover for fermentation; before application, adding trace elements, fermentation functional bacterial liquid, potassium sulfate and potassium dihydrogen phosphate to obtain the fermentation bacterial fertilizer. The method has low cost and simple preparation procedure, and the zymogen liquid obtained by one-time preparation can meet the requirement of large-scale planting. And the soybean meal in the additive can be fermented sufficiently and thoroughly, has the advantages of quick dissolution, complete hydrolysis, no residue and the like, is more beneficial to plant absorption or transformation, and reduces the scaling and blockage of a drip irrigation pipeline.)

1. The preparation method of the zymophyte fertilizer is characterized by comprising the following steps:

(1) adding a first portion of clear water into the fermentation tank, and heating to 95 ℃;

(2) adding bean flour, and cooking for 0.5-1 hour, wherein the mass ratio of the first part of clear water to the bean flour is 2: 1;

(3) adding a second part of clear water, and keeping the temperature of the liquid at 30-40 ℃; the volume ratio of the first part of clear water to the second part of clear water is 2: 1;

(4) adding brown sugar, amino acid, fermentation functional bacteria liquid and mineral fulvic acid, wherein the mass ratio of the bean flour to the brown sugar to the amino acid to the mineral fulvic acid is 10:2:10:1, and the volume ratio of the first clear water to the fermentation functional bacteria liquid is 400: 3;

(5) opening the cover and fermenting for 12 hours;

(6) before application, adding trace elements, fermentation functional bacterial liquid, potassium sulfate and monopotassium phosphate, wherein the mass ratio of the bean powder to the trace elements to the fermentation functional bacterial liquid to the potassium sulfate to the monopotassium phosphate is 20:1:2:4: 2; and (4) uniformly stirring, and then sieving with a 80-mesh sieve to obtain the fermented bacterial fertilizer.

2. The method according to claim 1, wherein the fermentation functional bacteria liquid in the steps (4) and (6) comprises EM bacteria, Bacillus megaterium, Bacillus mucilaginosus and Bacillus laterosporus, and photosynthetic bacteria or Trichoderma viride are added according to different seasons; wherein the mass ratio of the EM bacteria, the bacillus megaterium, the bacillus mucilaginosus and the bacillus laterosporus is 20:10:10: 1; photosynthetic bacteria are added in the early spring leaf-expanding period and the late autumn leaf-falling period, and the mass ratio of the bacillus laterosporus to the photosynthetic bacteria is 1: 0.5; trichoderma viride is added in summer, and the mass ratio of the bacillus laterosporus to the trichoderma viride is 1: 0.5.

3. A fermented bacterial manure obtained by the production method as claimed in claim 1 or 2.

4. The use method of the zymophyte as claimed in claim 3 is drip irrigation, root irrigation or foliage spraying.

5. The use method according to claim 4, characterized in that the specific operating method of the drip irrigation is as follows:

the drip irrigation pipeline is flushed with clear water, the fermented bacterial manure is diluted 1000 times in a drip irrigation fertilizer applicator for drip irrigation, and the drip irrigation pipeline is flushed with the clear water after the drip irrigation is finished, so that the pipeline blockage is prevented.

6. The use method of claim 4, wherein the specific operation method of the root irrigation is as follows:

diluting the fermented bacterial fertilizer 1000 times with clear water, and then irrigating the fermented bacterial fertilizer with roots or ditches to soil.

7. The use method of claim 4, wherein the specific operation method of foliar spray is as follows:

the fermentation bacterial fertilizer is diluted 3000 times by clear water, supernatant is taken after precipitation and sprayed on leaves, and high temperature and rain are avoided after spraying.

8. Use of the fermented bacterial manure obtained by the preparation method according to claim 1 or 2 in grapes, cherries, apples, Chinese cabbage and radishes.

Technical Field

The invention relates to a fermented bacterial fertilizer and a preparation method and application thereof, belonging to the technical field of bacterial fertilizers.

Background

In the protected field cultivation, the problems of no rain, inconvenience of deep ploughing, excessive fertilization, serious continuous cropping and the like generally exist, so that the soil environment is greatly changed in the open field, various physiological obstacles are continuously generated, the yield and the quality of plants are continuously reduced, and the production cost, the difficulty and the risk are increased. The main reasons for this are: (1) the self-toxicity of crops. Some crops can release substances through the ways of overground leaching, root secretion, crop residue decomposition and the like, and can generate inhibition effect on the growth and development of the same or next crop same or same family crops. Continuous cropping for many years has more and more obvious self-toxicity, continuous cropping obstacles appear, and the longer the continuous cropping time, the more serious the obstacles. (2) The beneficial microorganisms in the soil are reduced, the harmful microorganisms are increased, and the soil-borne diseases are aggravated. Due to continuous cropping, a special soil environment is formed, so that the growth and reproduction of beneficial microorganisms such as azotobacter, rhizobia, photosynthetic bacteria, actinomycetes, nitrobacteria, ammonifying bacteria, mycorrhizal fungi and the like are inhibited, a large number of harmful microorganisms are bred, the microbial flora of the soil is changed, soil-borne diseases are serious, and the phenomena of 'no treatment by using drugs' are frequently caused. (3) The soil property is deteriorated, secondary salinization and soil acidification are serious. Under the temptation of pursuing high yield in one area, high-content chemical fertilizers and animal wastes, human excreta and the like which are not decomposed are applied in large quantities, resulting in excessive salt content in soil. The salt can not be fully leached and accumulated in a soil plough layer in a large amount, secondary salinization and acidification of the soil are generated, the pH value is changed, various nutrients are not reserved according to relative proportions, and antagonism among elements influences the effectiveness of the soil nutrients to cause physiological diseases. (4) Root-knot nematodes are severe. Nematodes are parasites of crops and mainly damage the root systems of the crops and affect the normal growth of the crops. Therefore, the plant diseases and insect pests are aggravated, the soil secondary salinization and acidification are difficult to overcome, the plant autotoxicity or allelopathy is difficult to predict, and the element balance damage is difficult to correct in the protected field cultivation.

The microbial fertilizer is a novel fertilizer, contains microorganisms and available nutrients, can decompose invalid minerals and organic matters in soil through metabolic activity of living microorganisms, improves the nutritional conditions of plants, increases the supply of nutrient elements, improves the soil fertility, further stimulates the growth of crops, improves the quality of agricultural products, improves the soil state, enhances the stress resistance of the crops, and finally achieves the purpose of promoting the growth and development of the crops. It can be said that microbial fertilizers are the leading force of future fertilizers.

The action mechanism of the microbial fertilizer is different from that of a chemical fertilizer and mainly shows as follows: the physical and chemical properties of soil are improved, a microbial community is optimized, the utilization rate of the fertilizer is improved, and the fertilizer is green and pollution-free; comprehensive nutrition and lasting fertilizer efficiency; low cost and high benefit; is nontoxic and harmless to human, livestock and plants; can prevent and cure plant diseases and insect pests; the stress resistance of crops is increased; can effectively absorb nutrient resources in soil and improve crop yield. However, the microbial fertilizer is susceptible to interference from external conditions, and therefore, external environmental factors (temperature, salt concentration, and soil pH) should be taken into consideration when applying the microbial fertilizer. The microorganisms have various types, wide distribution, various functions and action mechanisms, strong adaptability and easy variation, so the application prospect of the microorganism is wider.

The microbial fertilizer has multiple functions, the core of the microbial fertilizer is microbes, and the microbial fertilizer greatly promotes the growth of crops, improves the soil structure and improves the crop yield. In a strict sense, the biological bacterial manure is not directly supplied with nutrients, but increases the nutrient supply in the soil through the life activities of specific microorganisms contained therein. The microbial fertilizer has the following functions:

1. promoting plant growth

The microbial fertilizer not only provides living microorganisms with special effects of dissolving phosphorus, potassium and fixing nitrogen, but also contains major elements such as nitrogen, phosphorus, potassium and the like, trace elements such as iron, copper, manganese, calcium, boron and the like, a large amount of organic matter nutrient components, a plurality of medium elements and humus and the like required by plant growth. This is not provided by chemical fertilizers. The nitrogen-fixing microorganism in the microbial fertilizer can fix nitrogen in the atmosphere, the nitrogen element content is improved, and the phosphorus-dissolving and potassium-dissolving microorganism can activate insoluble compounds (phosphorus and potassium) which cannot be utilized by plants into an element state which can be absorbed and utilized by the plants, so that the growth of the plants is promoted.

2. Enhancing stress resistance of plants

When plants are subjected to stress environments such as drought, diseases and the like, various bacterial strains contained in the microbial fertilizer can induce the plants to generate phenol oxidase, chitinase, peroxidase and the like to resist the stress, so that the resistance of the crops is improved, the microorganisms can form mycorrhiza at the roots, the crops are enhanced to absorb water and nutrients, the mycorrhiza becomes an advantageous population of the rhizosphere of the crops, and the drought resistance of the plants is enhanced.

3. Reducing and alleviating plant diseases and insect pests

Antagonism refers to the ability of microorganisms to produce metabolites that inhibit or kill phytopathogens. Research shows that one kind of microbe can produce active matter capable of inhibiting several kinds of pathogenic microbes. After the microbial fertilizer is applied to soil, a large amount of microbes exist in the soil of a plant root system, and can inhibit the growth of pathogenic microbes and induce plants to generate systemic disease resistance. Biocontrol bacteria and pathogenic microorganisms can generate competition effect, the biocontrol bacteria compete for nutrients in soil mutually, and the biocontrol bacteria consume a large amount of nutrient substances, so that the nutrient substances cannot be obtained when pathogenic bacteria invade, and infection fails. The microorganism in the microbial fertilizer forms dominant bacteria in the rhizosphere of crops, antagonizes other pathogenic microorganisms, is beneficial to resisting diseases and reducing the invasion of harmful microorganisms. Beneficial microorganisms in the fertilizer grow and reproduce, so that an advantageous population is formed in a crop rhizosphere soil micro-ecosystem, the growth and reproduction opportunities of other pathogenic microorganisms are limited, the fertilizer has the effect of antagonizing pathogenic microorganisms, and the effect of reducing crop diseases is achieved.

4. Improving crop yield and quality

In China, about 80% of land is lack of available phosphorus and about 60% of farmland is lack of potassium, in order to increase yield, people excessively depend on chemical fertilizers, biological fertilizers are rarely applied, a large amount of chemical fertilizers are applied, the chemical fertilizers cannot be absorbed and utilized by plants, one part of the chemical fertilizers are remained in the soil, and the other part of the chemical fertilizers flow into water, so that the utilization rate of the fertilizers is not high. The fertilizer is excessively applied, and although a large amount of phosphorus and potassium exist in the soil, the fertilizer is not beneficial to the growth of plants and can bring great negative effects to the soil.

The microbial compound fertilizer has the function of active microbes, and can release insoluble mineral substances in soil through the decomposition of azotobacter, phosphate solubilizing bacteria and potassium solubilizing bacteria, thereby promoting the substance circulation of the whole nature. The microorganism can produce various metabolites, such as hormones such as auxin, cytokinin, gibberellin and the like, and various vitamins, enzymes, organic acids and the like, and the hormones can stimulate the root system of the plant, further promote the growth of the plant, ensure that the plant grows well, and finally achieve the purpose of increasing the yield of the plant.

5. Improving the physical and chemical properties of soil

The state of the soil directly affects the growth of the crop. The microorganism in the microbial fertilizer must have safe, pollution-free and non-pathogenic bacteria, and the microorganism has no indexes of adverse effect on soil after being applied to the soil. Therefore, more reports have been made on the application of microorganisms to soil improvement. After the microbial fertilizer is applied, metabolites generated by microorganisms can decompose organic substances such as protein, cellulose, fat, pectin, hemicellulose, chitin and the like in soil, and increase soil humus, so that the soil is improved. In addition, microbial metabolic processes can produce carbohydrates which bind to gums in the soil and also bind to plant mucilage to loosen soil structure. Can improve the physical and chemical properties of soil, increase the granular structure of the soil and reduce the volume weight of the soil, thereby loosening the soil, eliminating the soil hardening caused by excessive use of chemical fertilizers, improving the utilization rate of the fertilizers and providing a proper environment for the growth of crops. The saline-alkali soil in China has wide area, and if the saline-alkali soil can be converted into the soil in which crops can grow by some methods, the problem of less cultivated land can be solved. The microbial fertilizer can generate a plurality of metabolites, so that the structure of soil is improved, such as saline-alkali soil is improved, the strains are mainly bacteria, fungi, actinomycetes and the like, and the principle is that the metabolites secreted by active microbes can absorb salt in the soil, so that organic matters are synthesized, inorganic nutrients are converted into organic nutrients, and the nutrients are fully utilized by crops. The microbial fertilizer also has the effects of reducing the content of nitrate, relieving the continuous cropping obstacle of crops, reducing the dosage of the fertilizer by 30-60%, improving the soil fertility, reducing the emission of greenhouse gases and producing non-toxic, harmless and pollution-free green organic crops.

Disclosure of Invention

Aiming at the problems, the invention provides the zymophyte fertilizer and the preparation method and the application thereof, the zymophyte fertilizer has the advantages of low cost and simple preparation procedure, and the mass of zymophyte liquid can be obtained by one-time preparation, thereby meeting the requirement of large-scale planting. The soybean flour in the additive can be fermented sufficiently and thoroughly, has the advantages of quick dissolution, complete hydrolysis, no residue and the like, is more beneficial to plant absorption or transformation, and reduces the scaling and blockage of a drip irrigation pipeline.

The technical scheme of the invention is as follows:

a preparation method of a zymocyte fertilizer comprises the following steps:

(1) adding a first portion of clear water into the fermentation tank, and heating to 95 ℃;

(2) adding bean flour, and cooking for 0.5-1 hour, wherein the mass ratio of the first part of clear water to the bean flour is 2: 1;

(3) adding a second part of clear water, and keeping the temperature of the liquid at 30-40 ℃; the volume ratio of the first part of clear water to the second part of clear water is 2: 1;

(4) adding brown sugar, amino acid, fermentation functional bacteria liquid and mineral fulvic acid, wherein the mass ratio of the bean flour to the brown sugar to the amino acid to the mineral fulvic acid is 10:2:10:1, and the volume ratio of the first clear water to the fermentation functional bacteria liquid is 400: 3;

(5) opening the cover and fermenting for 12 hours;

(6) before application, adding trace elements, fermentation functional bacterial liquid, potassium sulfate and monopotassium phosphate, wherein the mass ratio of the bean powder to the trace elements to the fermentation functional bacterial liquid to the potassium sulfate to the monopotassium phosphate is 20:1:2:4: 2; and (4) uniformly stirring, and then sieving with a 80-mesh sieve to obtain the fermented bacterial fertilizer.

Further, the fermentation functional bacteria liquid in the step (4) and the step (6) comprises EM bacteria, bacillus megaterium, bacillus mucilaginosus and bacillus laterosporus, and photosynthetic bacteria groups or trichoderma viride are selectively added according to different seasons; wherein the mass ratio of the EM bacteria, the bacillus megaterium, the bacillus mucilaginosus and the bacillus laterosporus is 20:10:10: 1; photosynthetic bacteria are added in the early spring leaf-expanding period and the late autumn leaf-falling period, the mass ratio of the bacillus laterosporus to the photosynthetic bacteria is 1:0.5, so that the growth of the leaves is accelerated or the photosynthetic time of the leaves is prolonged; and trichoderma viride is added in summer, and the mass ratio of the bacillus laterosporus to the trichoderma viride is 1:0.5, so that the cellulose degradation speed of field patrinia scabiosaefolia is accelerated.

The invention also comprises the fermented bacterial manure obtained by the method and a using method of the fermented bacterial manure.

The zymophyte fertilizer can be sprayed by drip irrigation, root irrigation or page spraying; the method comprises the following specific steps:

drip irrigation: the drip irrigation pipeline is flushed with clear water, the fermented bacterial manure is diluted 1000 times in a drip irrigation fertilizer applicator for drip irrigation, and the drip irrigation pipeline is flushed with the clear water after the drip irrigation is finished, so that the pipeline blockage is prevented.

Root irrigation: diluting the fermented bacterial fertilizer 1000 times with clear water, and then irrigating the fermented bacterial fertilizer with roots or ditches to soil.

And (3) foliage spraying: the fermentation bacterial fertilizer is diluted 3000 times by clear water, supernatant is taken after precipitation and sprayed on leaves, and high temperature and rain are avoided after spraying.

The invention also comprises the application of the fermented bacterial manure obtained by the method in grapes, cherries, apples, Chinese cabbages and radishes.

Compared with the prior art, the invention has the following advantages:

the invention can effectively promote the generation of carbohydrates and sugar conversion of fruits (grapes, apples and cherries), increase the content of soluble solids in the fruits, improve the quality of the fruits, effectively improve the quality and yield of the fruits and obviously increase the yield by 20-60% (different substances). Improve the quality of crops and agricultural products, improve the sugar degree of fruits, indirectly promote the coming into the market and increase the income of farmers. Can improve the contents of Chinese cabbage, radish carotene, riboflavin, vitamin C and protein to different degrees, reduce the content of crude fiber, and has better taste and more sufficient flavor. The fertilizer can reduce the fertilizer dosage by 30-60%, greatly improve the content of medium trace elements in soil, reduce the application amount of nitrogen, phosphorus and potassium and other medium trace elements, increase various efficient and active beneficial microbial bacteria, increase soil organic matters, accelerate the degradation and conversion of the organic matters into nutrient substances which can be absorbed by crops, greatly improve the soil fertility and reduce the fertilizer dosage. The healthy soil is reconstructed, the pest and disease resistance of crops is improved, the soil hardening is improved, the soil activity is stimulated, additional natural plant growth hormone and antibiotic are provided, the root system is developed, the absorption capacity is enhanced, and the immunity and the resistance of the crops are improved. Inhibit fungi and nematodes in soil and plant root diseases and insect pests, and reduce the use amount of pesticide. Promoting the growth and development of plants, improving the stress resistance, promoting the growth of root systems, and having the effects of orderly blooming, flower and fruit retention on fruit trees; late leaf-falling period and early spring disease resistance. Preventing and treating senilism, resisting continuous cropping, lodging, drought and cold. The method is non-toxic, harmless and pollution-free, is used for producing pollution-free, environment-friendly and green crops, and is particularly suitable for fresh grapes, apples, cherries and garden radish. The fertilizer has slow release, long effect and high energy, and has different fertilizer requirements in each period according to the characteristics of the fertilizer requirements of crops, so that the phenomenon of fertilizer release in the early stage, the vigorous growth and the later stage of the crops can be avoided.

Drawings

FIG. 1 shows the fruit bearing and growth conditions in the field of 4-year-old grapes (sun roses), with a yield per mu of about 3500 jin and a single spike weight of about 1.5-1.8 jin;

FIG. 2 shows the surface fertility and flora growth of 4-year old grapes (sun rose);

FIG. 3 shows the field fruit bearing and growth conditions of 2-year-old grapes (sun roses), with a yield per mu of about 800 jin and a single spike weight of about 1.0-1.2 jin;

FIG. 4 is a 2-year old grape (sun rose) single vine length (about 2.6 meters long, about 2.5 centimeters at its thickest diameter);

FIG. 5 shows the surface fertility and flora growth of 2-year old grapes;

FIG. 6 shows the overall growth of newly planted early-American cherries planted 10 months in 2020, with fruit bearing at 2-3 months in 2022 predicted; FIG. 7 shows the intercropping condition and under-mulch soil condition of new cherry planting in 2020;

FIG. 8 shows the overall growth of early-American cherry transplanted in 3 months in 2019 (conventional outdoor cultivation), and fruit bearing in 5-6 months in 2022 is expected;

FIG. 9 shows the row-to-row condition and the bud coarseness of cherry transplanting in 2019;

FIG. 10 shows the surface fertility and flora growth of the cherry transplanted in 2019;

FIG. 11 shows the growth of early-stage cherry transplanted in 3 months in 2017 (conventional outdoor cultivation), fruits are set and sold on the market in 5-6 months in 2021, and the yield per mu is 2000 jin;

FIG. 12 shows the growth of Venus gold transplanted in 3 months in 2017 (conventional open field cultivation), fruit begins to appear on the market in 8 months in 2020, yield per mu is about 1600 jin in 2021, and the gold can be stored for 3 months in a simple way.

Detailed Description

The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. The examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

Example 1: fermented bacterial fertilizer and preparation method and application thereof

The preparation method comprises the following steps:

(1) adding a first portion of clear water into the fermentation tank, and heating to 95 ℃;

(2) adding bean flour, and cooking for 1 hour, wherein the mass ratio of the first part of clear water to the bean flour is 2: 1;

(3) adding a second part of clear water, and keeping the liquid temperature at 35 ℃; the volume ratio of the first part of clear water to the second part of clear water is 2: 1;

(4) adding brown sugar, amino acid, fermentation functional bacteria liquid and mineral fulvic acid, wherein the mass ratio of the bean flour to the brown sugar to the amino acid to the mineral fulvic acid is 10:2:10:1, and the volume ratio of the first clear water to the fermentation functional bacteria liquid is 400: 3;

the fermentation functional bacterial liquid comprises EM bacteria, bacillus megatherium, bacillus mucilaginosus and bacillus laterosporus, and photosynthetic bacterial groups or trichoderma viride are selectively added according to different seasons; wherein the mass ratio of the EM bacteria, the bacillus megaterium, the bacillus mucilaginosus and the bacillus laterosporus is 20:10:10: 1; photosynthetic bacteria are added in the early spring leaf-expanding period and the late autumn leaf-falling period, the mass ratio of the bacillus laterosporus to the photosynthetic bacteria is 1:0.5, so that the growth of the leaves is accelerated or the photosynthetic time of the leaves is prolonged; adding trichoderma viride in summer, wherein the mass ratio of bacillus laterosporus to trichoderma viride is 1:0.5, so as to accelerate the cellulose degradation speed of field patrinia scabiosaefolia;

(5) opening the cover and fermenting for 12 hours;

(6) before application, adding trace elements, fermentation functional bacterial liquid, potassium sulfate and monopotassium phosphate, wherein the mass ratio of the bean powder to the trace elements to the fermentation functional bacterial liquid to the potassium sulfate to the monopotassium phosphate is 20:1:2:4: 2; and (4) uniformly stirring, and then sieving with a 80-mesh sieve to obtain the fermented bacterial fertilizer.

The fermentation bacterial fertilizer obtained by the preparation method is applied to grapes through drip irrigation; the drip irrigation pipeline is flushed with clear water, the fermented bacterial manure is diluted 1000 times in a drip irrigation fertilizer applicator for drip irrigation, and the drip irrigation pipeline is flushed with the clear water after the drip irrigation is finished, so that the pipeline blockage is prevented.

The results are shown in fig. 1-5, fig. 1 shows the fruit bearing and growth conditions in the field of 4-year-old grapes (sun roses), the yield per mu is about 3500 jin, and the weight of a single spike is about 1.5-1.8 jin; FIG. 2 shows the surface fertility and flora growth of 4-year old grapes; FIG. 3 shows the field fruit bearing and growth conditions of 2-year-old grapes (sun roses), with a yield per mu of about 800 jin and a single spike weight of about 1.0-1.2 jin; FIG. 4 is a single cane length (about 2.6 meters long, about 2.5 centimeters in thickest diameter); FIG. 5 shows the surface fertility and the growth of the bacterial flora of 2-year old grapes.

Example 2: a preparation method of a zymocyte fertilizer comprises the following steps:

(1) adding a first portion of clear water into the fermentation tank, and heating to 95 ℃;

(2) adding bean flour, and cooking for 0.5 hour, wherein the mass ratio of the first part of clear water to the bean flour is 2: 1;

(3) adding a second part of clear water, and keeping the liquid temperature at 30 ℃; the volume ratio of the first part of clear water to the second part of clear water is 2: 1;

(4) adding brown sugar, amino acid, fermentation functional bacteria liquid and mineral fulvic acid, wherein the mass ratio of the bean flour to the brown sugar to the amino acid to the mineral fulvic acid is 10:2:10:1, and the volume ratio of the first clear water to the fermentation functional bacteria liquid is 400: 3;

the fermentation functional bacterial liquid comprises EM bacteria, bacillus megatherium, bacillus mucilaginosus and bacillus laterosporus, and photosynthetic bacterial groups or trichoderma viride are selectively added according to different seasons; wherein the mass ratio of the EM bacteria, the bacillus megaterium, the bacillus mucilaginosus and the bacillus laterosporus is 20:10:10: 1; photosynthetic bacteria are added in the early spring leaf-expanding period and the late autumn leaf-falling period, the mass ratio of the bacillus laterosporus to the photosynthetic bacteria is 1:0.5, so that the growth of the leaves is accelerated or the photosynthetic time of the leaves is prolonged; adding trichoderma viride in summer, wherein the mass ratio of bacillus laterosporus to trichoderma viride is 1:0.5, so as to accelerate the cellulose degradation speed of field patrinia scabiosaefolia;

(5) opening the cover and fermenting for 12 hours;

(6) before application, adding trace elements, fermentation functional bacterial liquid, potassium sulfate and monopotassium phosphate, wherein the mass ratio of the bean powder to the trace elements to the fermentation functional bacterial liquid to the potassium sulfate to the monopotassium phosphate is 20:1:2:4: 2; and (4) uniformly stirring, and then sieving with a 80-mesh sieve to obtain the fermented bacterial fertilizer.

The fermentation bacterial fertilizer obtained by the preparation method is applied to the cherry by foliage spraying, 3000 times of the fermentation bacterial fertilizer is diluted by clear water, supernatant is taken after precipitation and sprayed on the leaves, and high temperature and rain are avoided after spraying.

The results are shown in fig. 6-11, fig. 6 shows the overall growth of newly planted early-American cherries in 10 months in 2020, and fruits are expected to appear on the market in 2-3 months in 2022; FIG. 7 shows the intercropping condition and under-mulch soil condition of new cherry planting in 2020; FIG. 8 shows the overall growth of early-American cherry transplanted in 3 months in 2019 (conventional outdoor cultivation), and fruit bearing in 5-6 months in 2022 is expected; FIG. 9 shows the row-to-row condition and the bud coarseness of cherry transplanting in 2019; FIG. 10 shows the surface fertility and flora growth of the cherry transplanted in 2019; fig. 11 shows the growth condition (open field conventional cultivation) of early-stage cherry transplanted in 3 months in 2017, fruits are set and sold on the market in 5-6 months in 2021, and the yield per mu is 2000 jin.

Example 3: fermented bacterial fertilizer and preparation method and application thereof

The preparation method comprises the following steps:

(1) adding a first portion of clear water into the fermentation tank, and heating to 95 ℃;

(2) adding bean flour, and cooking for 1 hour, wherein the mass ratio of the first part of clear water to the bean flour is 2: 1;

(3) adding a second part of clear water, and keeping the liquid temperature at 40 ℃; the volume ratio of the first part of clear water to the second part of clear water is 2: 1;

(4) adding brown sugar, amino acid, fermentation functional bacteria liquid and mineral fulvic acid, wherein the mass ratio of the bean flour to the brown sugar to the amino acid to the mineral fulvic acid is 10:2:10:1, and the volume ratio of the first clear water to the fermentation functional bacteria liquid is 400: 3;

the fermentation functional bacterial liquid comprises EM bacteria, bacillus megatherium, bacillus mucilaginosus and bacillus laterosporus, and photosynthetic bacterial groups or trichoderma viride are selectively added according to different seasons; wherein the mass ratio of the EM bacteria, the bacillus megaterium, the bacillus mucilaginosus and the bacillus laterosporus is 20:10:10: 1; photosynthetic bacteria are added in the early spring leaf-expanding period and the late autumn leaf-falling period, the mass ratio of the bacillus laterosporus to the photosynthetic bacteria is 1:0.5, so that the growth of the leaves is accelerated or the photosynthetic time of the leaves is prolonged; adding trichoderma viride in summer, wherein the mass ratio of bacillus laterosporus to trichoderma viride is 1:0.5, so as to accelerate the cellulose degradation speed of field patrinia scabiosaefolia;

(5) opening the cover and fermenting for 12 hours;

(6) before application, adding trace elements, fermentation functional bacterial liquid, potassium sulfate and monopotassium phosphate, wherein the mass ratio of the bean powder to the trace elements to the fermentation functional bacterial liquid to the potassium sulfate to the monopotassium phosphate is 20:1:2:4: 2; and (4) uniformly stirring, and then sieving with a 80-mesh sieve to obtain the fermented bacterial fertilizer.

The fermented bacterial manure obtained by the preparation method is applied to apples by root irrigation, is diluted 1000 times by clear water, and then is irrigated to soil by root irrigation or furrow irrigation.

As shown in FIG. 12, FIG. 12 shows the growth of gold in Vinus gold transplanted in 3 months in 2017 (conventional cultivation in open field), fruit appeared on the market in late 8 months in 2020, yield per mu was about 1600 jin in 2021, and the gold can be stored for 3 months in a simple manner.