阅读说明：本技术 细叶苦荬菜在防治西花蓟马或木虱方面的用途及其生物农药与制备方法 (Application of herba Ixeritis Denticulatae in preventing and treating Frankliniella occidentalis or psylla, and biopesticide and preparation method thereof ) 是由 刘畅 王芳 魏淑花 张蓉 马建华 黄文广 王颖 朱猛蒙 宋双 吴晓燕 于 2021-11-05 设计创作，主要内容包括：本发明“细叶苦荬菜在防治西花蓟马或木虱方面的用途及其生物农药与制备方法”属于生物农药领域。本发明提供细叶苦荬菜在防治西花蓟马或木虱方面的用途,并基于该用途提供以细叶苦荬菜精油为活性成分的生物农药及生物农药的制备方法。利用本发明的细叶苦荬菜Ixeris Cass新用途或采用本发明的生物农药可高效杀灭西花蓟马或木虱,且细叶苦荬菜为野生植物,分布范围广泛,简单易得,成本低廉,适于大规模推广应用。(The invention relates to application of Ixeris denticulata in controlling Frankliniella occidentalis or psylla, a biological pesticide and a preparation method thereof, belonging to the field of biological pesticides. The invention provides application of Ixeris denticulata in controlling Frankliniella occidentalis or psylla, and provides a biological pesticide taking Ixeris denticulata essential oil as an active ingredient based on the application and a preparation method of the biological pesticide. The Ixeris denticulata Ixeris Cass new application or the biopesticide can efficiently kill the Frankliniella occidentalis or the psylla chinensis, and the Ixeris denticulata is a wild plant, has wide distribution range, is simple and easy to obtain, has low cost, and is suitable for large-scale popularization and application.)

1. The use of herba Ixeritis Denticulatae in preventing and treating Frankliniella occidentalis or psylla.

2. The use of Ixeris denticulata as claimed in claim 1 for controlling Frankliniella occidentalis or psylla, characterized in that essential oil of Ixeris denticulata is used to kill or kill Frankliniella occidentalis or psylla.

3. Use of the ixeris denticulata as claimed in claim 1 or 2 for controlling the thrips occidentalis or psyllid, characterized in that the active ingredients of the essential oil of ixeris denticulata comprise: (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid, (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid;

and/or the active ingredients of the Ixeris denticulata essential oil comprise the following substances in percentage by weight: 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, 1.2495% 2-methyltetracosane, 2.3619% docosahexaenoic acid, 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, and, 1.2495% of 2-methyl tetracosane and 2.3619% of docosahexaenoic acid.

4. Use of endives according to any of claims 1 to 3 for the control of thrips occidentalis or psyllid, characterized in that the killing means contact or fumigation;

and/or, the killing refers to killing the frankliniella occidentalis or the psylla chinensis by using the solution of the essential oil of the ixeris sonchifolia;

and/or the semilethal concentration of the herba ixeris denticulatae essential oil solution for killing the frankliniella occidentalis is 0.32mg/L,

and/or the semilethal concentration of the Ixeris denticulata essential oil solution for killing the psylla chinensis is 0.12 mu g/head;

and/or the herba Ixeritis Denticulatae essential oil solution is prepared by dissolving herba Ixeritis Denticulatae essential oil in alcohol.

5. A biopesticide, comprising: an active ingredient; the active ingredients comprise herba Ixeritis Denticulatae essential oil.

6. The biopesticide according to claim 5, wherein the active ingredients of the Ixeris denticulata essential oil comprise: (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid, (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid;

and/or the active ingredients of the Ixeris denticulata essential oil comprise the following substances in percentage by weight: 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, 1.2495% 2-methyltetracosane, 2.3619% docosahexaenoic acid, 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, and, 1.2495% of 2-methyl tetracosane and 2.3619% of docosahexaenoic acid.

7. The biopesticide of claim 5 or 6, further comprising: pharmaceutically acceptable adjuvants;

and/or the active component of the biological pesticide is an alcohol solution of the Ixeris denticulata essential oil;

and/or the killing or lethal object of the biological pesticide is Frankliniella occidentalis or psyllid.

8. A preparation method of biological pesticide is characterized in that the essential oil of Ixeris denticulata is used as the active ingredient of the biological pesticide.

9. The method for preparing a biopesticide according to claim 8, wherein the essential oil of Ixeris denticulata is obtained by extracting Ixeris denticulata with steam distillation method.

10. The method for preparing a biopesticide according to claim 8 or 9, wherein the aerial parts of the plant of the ixeris denticulata are dried and pulverized and then extracted by steam distillation;

and/or the extraction time is 4-12h, preferably 6h, and the extraction temperature is 70-120 ℃, preferably 100 ℃;

and/or the extracted extractant is n-hexane; the extraction time is 2-8h, preferably 6h, and the extraction temperature is 15-35 ℃, preferably 25 ℃;

and/or drying and concentrating the n-hexane layer obtained after extraction;

and/or, the drying refers to removing water with anhydrous sodium sulfate;

and/or, the concentration refers to that the dried product is evaporated by a rotary evaporator to remove n-hexane to obtain the Ixeris denticulata essential oil.

Technical Field

The invention belongs to the field of biological pesticides, and particularly relates to application of Ixeris denticulata in controlling Frankliniella occidentalis or psylla chinensis, a biological pesticide and a preparation method thereof.

Background

Ixeris denticulata Ixeris Cass is perennial herb, has white milk and has no hair. Thin root and fibrous. The florescence is 6-9 months, the fruit period is 7-10 months, and the distribution is in many places.

Frankliniella occidentalis (Pergrande) is a species of animal of the family Thripidae of the order Thysanoptera of the class Insecta, Arthropoda, the family Arthropoda. Also known as alfalfa thrips, belongs to the order Thysanoptera. Frankliniella occidentalis omnivorous, host plants are known to be as many as 500 over.

Psyllid, a Psyllidae, is an insect of the family homoptera Psyllidae of the class entomophyda, the phylum Arthropoda, a species of the asymptomatic type, with the individual developing through three stages, the egg, the nymph and the adult. The mouth organ is of piercing-sucking type. The adult is small in size and is lively and jumping well. If the body is round and flat, waxy secretions cover the body surface. Psyllids are a woody plant pest.

The research on the control of the frankliniella denticulata or the psylla chinensis by the Ixeris denticulata is not reported in the field.

Disclosure of Invention

Aiming at the blank in the field, the invention provides the application of the Ixeris denticulata in the aspect of controlling the Frankliniella occidentalis or the psylla chinensis.

The technical scheme of the invention is as follows:

the use of herba Ixeritis Denticulatae in preventing and treating Frankliniella occidentalis or psylla.

The essential oil of the ixeris sonchifolia is adopted to kill or kill the frankliniella occidentalis or the psylla.

The active ingredients of the Ixeris denticulata essential oil comprise: (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid, (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid;

preferably, the active ingredients of the ixeris denticulata essential oil comprise the following substances in percentage by weight: 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, 1.2495% 2-methyltetracosane, 2.3619% docosahexaenoic acid, 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, and, 1.2495% of 2-methyl tetracosane and 2.3619% of docosahexaenoic acid.

The killing refers to contact killing or fumigating killing;

preferably, the killing refers to killing the frankliniella occidentalis or the psylla chinensis by using the solution of the essential oil of the ixeris sonchifolia;

preferably, the semilethal concentration of the solution of the Ixeris denticulata essential oil for killing the frankliniella occidentalis is 0.32mg/L,

preferably, the semilethal concentration of the Ixeris denticulata essential oil solution for killing psylla chinensis is 0.12 μ g/head;

preferably, the said solution of essential oil of Ixeris denticulata is a solution prepared by dissolving essential oil of Ixeris denticulata in alcohol.

A biopesticide, comprising: an active ingredient; the active ingredients comprise herba Ixeritis Denticulatae essential oil.

The active ingredients of the Ixeris denticulata essential oil comprise: (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid, (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid;

preferably, the active ingredients of the ixeris denticulata essential oil comprise the following substances in percentage by weight: 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, 1.2495% 2-methyltetracosane, 2.3619% docosahexaenoic acid, 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, and, 1.2495% of 2-methyl tetracosane and 2.3619% of docosahexaenoic acid.

The biological pesticide also comprises: pharmaceutically acceptable adjuvants;

preferably, the active ingredient of the biological pesticide is an alcohol solution of the essential oil of the ixeris denticulata;

preferably, the killing or lethal object of the biological pesticide is frankliniella occidentalis or psyllid.

A preparation method of biological pesticide is characterized in that the essential oil of Ixeris denticulata is used as the active ingredient of the biological pesticide.

The said essential oil is extracted from herba Ixeritis Denticulatae by steam distillation.

Drying and pulverizing aerial parts of herba Ixeritis Denticulatae, and extracting by steam distillation;

preferably, the extraction time is 4-12h, preferably 6h, and the extraction temperature is 70-120 ℃, preferably 100 ℃;

preferably, the extracted extractant is n-hexane; the extraction time is 2-8h, preferably 6h, and the extraction temperature is 15-35 ℃, preferably 25 ℃;

preferably, drying and concentrating the n-hexane layer obtained after extraction;

preferably, the drying refers to removing water with anhydrous sodium sulfate;

preferably, the concentration refers to that the normal hexane of the dried product is removed through evaporation by a rotary evaporator to obtain the Ixeris denticulata essential oil.

The invention discovers and claims a new application of Ixeris denticulata Ixeris cases in controlling Frankliniella occidentalis or psylla chinensis for the first time, and the new application is realized mainly by fumigating or killing Frankliniella denticulata or psylla chinensis with Ixeris denticulata essential oil. The invention also extracts and identifies the active insecticidal components in Ixeris denticulata Ixeris Cass essential oil for the first time, which comprise (4-ethylphenyl) -trimethylsilane, caprylic acid, pelargonic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid, (4-ethylphenyl) -trimethylsilane, caprylic acid, pelargonic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, Docosahexaenoic acid, and the like. Meanwhile, the high lethal efficiency of the Ixeris denticulata case essential oil on the Frankliniella occidentalis or the psyllid is proved by a lethal test, and the half lethal concentration of the Ixeris denticulata case essential oil is obviously lower than that of the conventional pesticide. The Ixeris denticulata Ixeris Cass new application or the biopesticide can efficiently kill the Frankliniella occidentalis or the psylla chinensis, and the Ixeris denticulata is a wild plant, has wide distribution range, is simple and easy to obtain, has low cost, and is suitable for large-scale popularization and application.

Detailed Description

The present invention will be described in further detail with reference to specific examples, but the scope of the present invention is not limited thereto.

Group 1 example, New use of Ixeris denticulata of the present invention

The group of embodiments provides the application of the Ixeris denticulata in the aspect of controlling the Frankliniella occidentalis or the psylla chinensis.

In some embodiments, the use refers to: the essential oil of the ixeris sonchifolia is adopted to kill or kill the frankliniella occidentalis or the psylla.

In a specific embodiment, the active ingredients of the ixeris denticulata essential oil comprise: (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid, (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid;

preferably, the active ingredients of the ixeris denticulata essential oil comprise the following substances in percentage by weight: 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, 1.2495% 2-methyltetracosane, 2.3619% docosahexaenoic acid, 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, and, 1.2495% of 2-methyl tetracosane and 2.3619% of docosahexaenoic acid.

In other embodiments, the killing refers to contact killing or fumigation;

preferably, the killing refers to killing the frankliniella occidentalis or the psylla chinensis by using the solution of the essential oil of the ixeris sonchifolia;

preferably, the semilethal concentration of the solution of the Ixeris denticulata essential oil for killing the frankliniella occidentalis is 0.32mg/L,

preferably, the semilethal concentration of the Ixeris denticulata essential oil solution for killing psylla chinensis is 0.12 μ g/head;

preferably, the said solution of essential oil of Ixeris denticulata is a solution prepared by dissolving essential oil of Ixeris denticulata in alcohol.

Group 2 examples of biopesticides of the present invention

The present group of embodiments provides a biopesticide. All embodiments of this group share the following common features: the biological pesticide comprises: an active ingredient; the active ingredients comprise herba Ixeritis Denticulatae essential oil.

In some embodiments, the active ingredients of the ixeris denticulata essential oil include: (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid, (4-ethylphenyl) -trimethylsilane, octanoic acid, nonanoic acid, 2-methoxy-3- (2-propenyl) -phenol, lauric acid, 6,10, 14-trimethyl-2-pentadecanone, myristic acid, n-pentadecanoic acid, nonadecane, linolenic acid, 2-methyltetracosane, docosahexaenoic acid;

preferably, the active ingredients of the ixeris denticulata essential oil comprise the following substances in percentage by weight: 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, 1.2495% 2-methyltetracosane, 2.3619% docosahexaenoic acid, 3.0026% (4-ethylphenyl) -trimethylsilane, 1.8249% octanoic acid, 2.9457% nonanoic acid, 1.0761% 2-methoxy-3- (2-propenyl) -phenol, 8.9251% lauric acid, 2.9534% 6,10, 14-trimethyl-2-pentadecanone, 3.3876% myristic acid, 37.767% n-pentadecanoic acid, 1.5567% nonadecane, 14.0451% linolenic acid, and, 1.2495% of 2-methyl tetracosane and 2.3619% of docosahexaenoic acid.

In a further embodiment, the biopesticide further comprises: pharmaceutically acceptable adjuvants; the skilled person can select or adjust various adjuvants according to the pesticide dosage form to be prepared. For example, the adjuvant may be selected from: solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesives, integration agents, permeation enhancers, pH regulators, buffers, plasticizers, surfactants, foaming agents, antifoaming agents, thickeners, encapsulation agents, humectants, absorbents, diluents, flocculants, deflocculants, filter aids, release retardants.

In some embodiments, the solvent may be ethanol.

Preferably, the active ingredient of the biological pesticide is an alcohol solution of the essential oil of the ixeris denticulata;

preferably, the target for killing or killing the biological pesticide is frankliniella occidentalis or psyllid.

Group 3 example, Process for the preparation of the biopesticides of the present invention

The present group of embodiments provides a method for preparing a biopesticide. All embodiments of this group share the following common features: the essential oil of Ixeris denticulata is used as the active component of the biological pesticide.

In a specific embodiment, the said essential oil is extracted from herba Ixeritis Denticulatae by steam distillation.

In a more specific embodiment, aerial parts of Ixeris denticulata are dried and pulverized, and then extracted by steam distillation;

preferably, the extraction time is 4-12h, preferably 6h, and the extraction temperature is 70-120 ℃, preferably 100 ℃;

preferably, the extracted extractant is n-hexane; the extraction time is 2-8h, preferably 6h, and the extraction temperature is 15-35 ℃, preferably 25 ℃;

preferably, drying and concentrating the n-hexane layer obtained after extraction;

preferably, the drying refers to removing water with anhydrous sodium sulfate;

preferably, the concentration refers to that the normal hexane of the dried product is removed through evaporation by a rotary evaporator to obtain the Ixeris denticulata essential oil. Experimental example, preparation of Ixeris denticulata essential oil and insect disinfestation test

1. Preparation of plant essential oil

Drying aerial parts of herba Ixeritis Denticulatae in the shade, pulverizing, weighing, extracting with water vapor distillation for 6 hr to obtain n-hexane layer, adding anhydrous sodium sulfate to remove water, concentrating, measuring volume, placing volatile oil (herba Ixeritis Denticulatae essential oil) in dry glass bottle, and storing in refrigerator at 4 deg.C.

2. GC-MS testing

The volatile oil sample adopts GC-MS to analyze the component composition of the sample, and the test conditions are as follows: GCMS-QP2010 plus (Shimadzu); a chromatographic column: DB-5MS (60m 0.25um 0.32mm) (Agilent); the heating rate is as follows: 60-10 ℃/min-300-20 min; the split ratio is as follows: 30: 1; constant linear velocity mode: 30 cm/sec; carrier gas: he; ion source temperature: 200 ℃; interface temperature: 300 ℃; sample inlet temperature: 320 ℃; mass scan range: m/z 29-650 sample size: 1 mu L of the solution; sample pretreatment: filtering the sample by a filter head containing a 0.22 mu m organic filter membrane of a disposable syringe; and (3) searching a spectrum library: the Nist 2017 standard library.

The components of the Ixeris denticulata essential oil are shown in the following table 1 after detection:

TABLE 1

The reason why the total percentage of all the components identified in the above table 1 is less than 100% is that unknown components still exist in the essential oil of Ixeris denticulata, which may be new compounds, cannot be identified by the existing GC-MS detection method.

4. Determination of fumigating activity of herba Ixeritis Denticulatae volatile oil on Frankliniella occidentalis

A fumigation method is adopted, a 20mL small bottle is taken to suck 10 adult frankliniella occidentalis, the adult frankliniella occidentalis is sleeved with gauze and placed into a 250mL wide-mouth bottle, a 1cm multiplied by 6cm filter paper strip is fixed in the bottle cap, the filter paper strip with 20 mu L of liquid medicine (namely the solution of the Ixeris tenuifolia essential oil prepared in the step 1 dissolved in ethanol) dropped is aired for 10-20s, the bottle cap is covered after the solvent is volatilized, the filter paper strip is suspended in the center of the wide-mouth bottle, and the wide-mouth bottle cap is wrapped with a sealing film. After treatment, the jar is placed in a plant incubator, and the temperature (25 +/-0.5) DEG C, the relative humidity 50%, the illumination L: d is 16h:8h of illumination climate box. The negative and positive controls are negative and positive, respectively, with no volatile oil solvent and neem oil added. Repeating each treatment for 6 times, repeating each time for 10 thrips, checking death and survival condition of thrips in each treatment 24 hr later, calculating death rate, and calculating semilethal concentration LC by DPS₅₀。

5. Determination of contact killing activity of herba Ixeritis Denticulatae volatile oil on psyllid

The contact activity was determined by the microdroplet method. The test insect is paralyzed for 10min in a freezing box, 10 psyllids/tube, and the medicinal liquid (i.e. the solution of the Ixeris denticulata essential oil prepared in the step 1 dissolved in ethanol) is dripped on the forethorax and back plate of the insect body by adopting a dripping method, wherein 2 mu L of the medicinal liquid is dripped into each head. And (3) placing the treated psyllids in a centrifuge tube filled with the fresh and tender branches and buds of the Chinese wolfberry, and sealing the centrifuge tube by using a gauze. After treatment, the centrifuge tube is placed in an intelligent artificial illumination climate box, and the illumination climate box is set at a temperature (25 +/-0.5 ℃), has a relative humidity of 60% and has an illumination L: D of 16h:8 h. Negative and positive controls are set with solvent without volatile oil and neem oil. Repeating each treatment for 6 times, repeating 10 psyllids, checking death and survival condition of each treated psyllid 24h after treatment, calculating death rate, and calculating semi-lethal concentration LC by DPS₅₀/LD₅₀。

The comparative results are shown in table 2 below:

TABLE 2