阅读说明：本技术 脑磁数据采集分析方法及系统 (Magnetoencephalography data acquisition and analysis method and system ) 是由 高阳 梁晓钰 宁晓琳 房建成 于 2021-11-25 设计创作，主要内容包括：本申请提供一种脑磁数据采集分析方法及系统,涉及医学技术领域。该方法包括：应用于基于原子磁强计的脑磁数据采集分析系统,系统包括：刺激发生装置、脑磁数据采集装置和脑磁数据分析装置,脑磁数据采集装置和脑磁数据分析装置连接,刺激发生装置、脑磁数据采集装置均作用于受试者,该方法包括：脑磁数据采集装置采集受试者在刺激发生装置的刺激下产生的脑磁数据,脑磁数据分析装置接收脑磁数据采集装置传输的所述脑磁数据,并对脑磁数据进行分析,生成多种脑磁分析结果。本申请可实现针对原子磁强计提供完整的脑磁分析方法和系统,便于进行实验和临床分析。(The application provides a magnetoencephalography data acquisition and analysis method and system, and relates to the technical field of medicine. The method comprises the following steps: be applied to brain magnetism data acquisition analytic system based on atomic magnetometer, the system includes: the brain magnetic data acquisition device is connected with the brain magnetic data analysis device, and the stimulation generation device and the brain magnetic data acquisition device act on a testee, and the method comprises the following steps: the magnetoencephalography data acquisition device acquires magnetoencephalography data generated by a testee under the stimulation of the stimulation generation device, and the magnetoencephalography data analysis device receives the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device and analyzes the magnetoencephalography data to generate a plurality of magnetoencephalography analysis results. The method and the system can provide a complete magnetoencephalography analysis method and a complete magnetoencephalography analysis system for the atomic magnetometer, and are convenient for experiment and clinical analysis.)

1. A magnetoencephalography data acquisition and analysis method is applied to a magnetoencephalography data acquisition and analysis system based on an atomic magnetometer, and the magnetoencephalography data acquisition and analysis system comprises: the brain magnetic data acquisition device is connected with the brain magnetic data analysis device, and the stimulation generation device and the brain magnetic data acquisition device both act on a subject, and the method comprises the following steps:

the magnetoencephalography data acquisition device acquires magnetoencephalography data generated by the subject under stimulation of the stimulation generation device, and the magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points;

the magnetoencephalography data analysis device receives the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device, analyzes the magnetoencephalography data and generates a plurality of magnetoencephalography analysis results, and the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic intensity real-time oscillogram and the whole brain real-time topographic map of the plurality of test points are distribution maps of the whole brain magnetic intensity at different moments, which are obtained according to the brain magnetic intensity real-time oscillogram of the plurality of test points.

2. The method of claim 1, wherein the magnetoencephalography data acquisition and analysis system further comprises: an analysis results report generating device, the method further comprising:

the analysis result report generation device imports the questionnaire test result of the subject and receives the magnetoencephalography analysis result;

and the analysis result report generating device performs health analysis on the subject according to the questionnaire test result and the magnetoencephalography analysis result to obtain a health analysis result.

3. The method of claim 2, wherein the analysis result report generation device performs a health analysis on the subject to obtain a health analysis result according to the questionnaire test result and the magnetoencephalography analysis result, and comprises:

matching the questionnaire test results of the same type with the magnetoencephalography analysis results to obtain matching degree parameters;

and acquiring a health analysis result according to the matching degree parameter.

4. The method of claim 1, wherein the magnetoencephalography data analysis device analyzes the magnetoencephalography data to generate a plurality of magnetoencephalography analysis results, comprising:

and the magnetoencephalography data analysis device calculates the magnetoencephalography intensity of the test points to obtain the magnetoencephalography intensity of a plurality of brain areas of the whole brain, and generates the magnetoencephalography intensity of the plurality of brain areas into the real-time topographic map of the whole brain.

5. The method of claim 1, wherein the method further comprises:

the magnetoencephalography data analysis device acquires multiple groups of magnetoencephalography data acquired by the magnetoencephalography data acquisition device based on multiple times of stimulation, and each group of magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points at different moments; calculating to obtain an evoked response field parameter of each test point according to a plurality of magnetoencephalography intensities of each test point at the same time, wherein the evoked response field parameter comprises: the magnetoencephalography intensity peak value, the peak value moment, the peak value interval and the magnetoencephalography intensity average value at the same moment of each test point; and drawing the average value of the magnetoencephalography at the same moment as an evoked response field map of each test point.

6. The method of claim 4, wherein the method further comprises:

the magnetoencephalography data analysis device calculates a correlation of magnetoencephalography intensities of the plurality of brain regions; drawing the correlation among the brain areas on the brain scanning structure chart to obtain a brain function network; and determining the connection strength between any two brain areas according to the correlation among the brain areas in the brain function network.

7. The method of claim 4, wherein the method further comprises:

the magnetoencephalography data analysis device calculates stimulation divergence directions and stimulation divergence intensities of magnetoencephalography intensities of the plurality of brain areas; drawing stimulation divergence directions and stimulation divergence intensities of the brain areas on a brain scanning structure chart to obtain an effect network; and determining the activity between any two brain areas according to the stimulation divergence intensity of the brain areas in the factor network.

8. The method of claim 1, wherein the magnetoencephalography data acquisition and analysis system further comprises: eye movement detection means and motion detection means; the method further comprises the following steps:

detecting eye movement data of the subject by the eye movement detection device;

detecting, by the motion detection device, motion data of the subject;

before the magnetoencephalography data analysis device analyzes the magnetoencephalography data to generate a plurality of magnetoencephalography analysis results, the method further comprises:

and the magnetoencephalography data analysis device denoises the magnetoencephalography data according to the eye movement data and the motion data.

9. A magnetoencephalography data collection and analysis system, applied to an atomic magnetometer, the system comprising: the brain magnetic data acquisition device is connected with the brain magnetic data analysis device, and the stimulation generation device and the brain magnetic data acquisition device act on a subject;

the magnetoencephalography data acquisition device is used for acquiring magnetoencephalography data generated by the subject under the stimulation of the stimulation generation device, and the magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points;

the magnetoencephalography data analysis device is used for receiving the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device, analyzing the magnetoencephalography data and generating a plurality of magnetoencephalography analysis results, wherein the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic intensity real-time oscillogram and the whole brain real-time topographic map of the plurality of test points are distribution maps of the whole brain magnetic intensity at different moments, which are obtained according to the brain magnetic intensity real-time oscillogram of the plurality of test points.

10. A magnetoencephalography data collection and analysis method, applied to a magnetoencephalography data analysis device in the system of claim 9, the method comprising:

receiving the magnetoencephalography data transmitted by a magnetoencephalography data acquisition device; wherein the magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points generated by a test subject under the stimulation of a stimulation generating device;

analyzing the magnetoencephalography data to generate a plurality of magnetoencephalography analysis results, wherein the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic intensity real-time oscillogram and the whole brain real-time topographic map of the plurality of test points are distribution maps of the whole brain magnetic intensity at different moments, which are obtained according to the brain magnetic intensity real-time oscillogram of the plurality of test points.

Technical Field

The invention relates to the technical field of medicine, in particular to a magnetoencephalography data acquisition and analysis method and system.

Background

The Magnetoencephalogram (MEG) is a non-invasive neuroimaging technique that directly measures the magnetic field generated by the human brain and can be used to diagnose a variety of brain functional diseases based on the magnetoencephalogram.

In the prior art, a brain magnetic strategy is performed by adopting a superconducting quantum interference device (SQUID), but the application of the SQUID has certain limitation. The SQUID equipment can work only by cooling the superconducting sensor to 4K (-269 ℃) by adopting liquid nitrogen, so that the maintenance cost is high. Meanwhile, SQUID devices require the use of rigid dewars to isolate the superconducting sensors from room temperature, and therefore, the requirement for effective thermal insulation between the superconducting sensors and the subject's head results in increased neuron-to-sensor distances, limiting the signal-to-noise ratio of magnetoencephalography.

In order to solve the problems of the superconducting sensor, in the prior art, a SERF (Spin-Exchange Relaxation-Free) atomic magnetometer is adopted to perform a magnetoencephalography experiment, but a complete system from stimulation generation to magnetoencephalography analysis is not formed in magnetoencephalography analysis of the atomic magnetometer at present, and the experimental and clinical general practicability is not provided.

Disclosure of Invention

The present invention aims to provide a magnetoencephalography data acquisition and analysis method and system, so as to provide a complete magnetoencephalography analysis method and system for an atomic magnetometer, which is convenient for experiments and clinical analysis.

In order to achieve the above purpose, the technical solutions adopted in the embodiments of the present application are as follows:

in a first aspect, an embodiment of the present application provides a magnetoencephalography data acquisition and analysis method, which is applied to a magnetoencephalography data acquisition and analysis system based on an atomic magnetometer, where the magnetoencephalography data acquisition and analysis system includes: the brain magnetic data acquisition device is connected with the brain magnetic data analysis device, and the stimulation generation device and the brain magnetic data acquisition device both act on a subject, and the method comprises the following steps:

the magnetoencephalography data acquisition device acquires magnetoencephalography data generated by the subject under stimulation of the stimulation generation device, and the magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points;

the magnetoencephalography data analysis device receives the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device, analyzes the magnetoencephalography data and generates a plurality of magnetoencephalography analysis results, and the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic intensity real-time oscillogram and the whole brain real-time topographic map of the plurality of test points are distribution maps of the whole brain magnetic intensity at different moments, which are obtained according to the brain magnetic intensity real-time oscillogram of the plurality of test points.

Optionally, the system for acquiring and analyzing magnetoencephalography data further includes: an analysis results report generating device, the method further comprising:

the analysis result report generation device imports the questionnaire test result of the subject and receives the magnetoencephalography analysis result;

and the analysis result report generating device performs health analysis on the subject according to the questionnaire test result and the magnetoencephalography analysis result to obtain a health analysis result.

Optionally, the analysis result report generating device performs health analysis on the subject according to the questionnaire test result and the magnetoencephalography analysis result to obtain a health analysis result, and includes:

matching the questionnaire test results of the same type with the magnetoencephalography analysis results to obtain matching degree parameters;

and acquiring a health analysis result according to the matching degree parameter.

Optionally, the magnetoencephalography data analysis device analyzes the magnetoencephalography data to generate a plurality of magnetoencephalography analysis results, including:

and the magnetoencephalography data analysis device calculates the magnetoencephalography intensity of the test points to obtain the magnetoencephalography intensity of a plurality of brain areas of the whole brain, and generates the magnetoencephalography intensity of the plurality of brain areas into the real-time topographic map of the whole brain.

Optionally, the method further includes:

the magnetoencephalography data analysis device acquires multiple groups of magnetoencephalography data acquired by the magnetoencephalography data acquisition device based on multiple times of stimulation, and each group of magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points at different moments; calculating to obtain an evoked response field parameter of each test point according to a plurality of magnetoencephalography intensities of each test point at the same time, wherein the evoked response field parameter comprises: the magnetoencephalography intensity peak value, the peak value moment, the peak value interval and the magnetoencephalography intensity average value at the same moment of each test point; and drawing the average value of the magnetoencephalography at the same moment as an evoked response field map of each test point.

Optionally, the method further includes:

the magnetoencephalography data analysis device calculates a correlation of magnetoencephalography intensities of the plurality of brain regions; drawing the correlation among the brain areas on the brain scanning structure chart to obtain a brain function network; and determining the connection strength between any two brain areas according to the correlation among the brain areas in the brain function network.

Optionally, the method further includes:

the magnetoencephalography data analysis device calculates stimulation divergence directions and stimulation divergence intensities of magnetoencephalography intensities of the plurality of brain areas; drawing stimulation divergence directions and stimulation divergence intensities of the brain areas on the brain scanning structure chart to obtain a cause-effect network; and determining the activity between any two brain areas according to the stimulation divergence intensity of the brain areas in the factor network.

Optionally, the system for acquiring and analyzing magnetoencephalography data further includes: eye movement detection means and motion detection means; the method further comprises the following steps:

detecting eye movement data of the subject by the eye movement detection device;

detecting, by the motion detection device, motion data of the subject;

before the magnetoencephalography data analysis device analyzes the magnetoencephalography data to generate a plurality of magnetoencephalography analysis results, the method further comprises:

and the magnetoencephalography data analysis device denoises the magnetoencephalography data according to the eye movement data and the motion data.

In a second aspect, the present application further provides a magnetoencephalography data acquisition and analysis system applied to an atomic magnetometer, the system comprising: the brain magnetic data acquisition device is connected with the brain magnetic data analysis device, and the stimulation generation device and the brain magnetic data acquisition device act on a subject;

the magnetoencephalography data acquisition device is used for acquiring magnetoencephalography data generated by the subject under the stimulation of the stimulation generation device, and the magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points;

the magnetoencephalography data analysis device is used for receiving the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device, analyzing the magnetoencephalography data and generating a plurality of magnetoencephalography analysis results, wherein the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic intensity real-time oscillogram and the whole brain real-time topographic map of the plurality of test points are distribution maps of the whole brain magnetic intensity at different moments, which are obtained according to the brain magnetic intensity real-time oscillogram of the plurality of test points.

In a third aspect, the present application further provides a magnetoencephalography data acquisition and analysis method, applied to a magnetoencephalography data analysis device in the system, the method including:

receiving the magnetoencephalography data transmitted by a magnetoencephalography data acquisition device; wherein the magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points generated by a test subject under the stimulation of a stimulation generating device;

analyzing the magnetoencephalography data to generate a plurality of magnetoencephalography analysis results, wherein the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic intensity real-time oscillogram and the whole brain real-time topographic map of the plurality of test points are distribution maps of the whole brain magnetic intensity at different moments, which are obtained according to the brain magnetic intensity real-time oscillogram of the plurality of test points.

The beneficial effect of this application is:

the application provides a magnetoencephalography data acquisition and analysis method and a system, wherein the method is applied to a magnetoencephalography data acquisition and analysis system based on an atomic magnetometer, and the system comprises: the brain magnetic data acquisition device is connected with the brain magnetic data analysis device, and the stimulation generation device and the brain magnetic data acquisition device both act on a testee, and the method comprises the following steps: the magnetoencephalography data acquisition device acquires magnetoencephalography data generated by a testee under the stimulation of the stimulation generation device, and the magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points; the magnetoencephalography data analysis device receives the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device, analyzes the magnetoencephalography data and generates a plurality of magnetoencephalography analysis results, and the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic strength real-time oscillogram and the whole brain real-time topographic map of the plurality of test points, wherein the whole brain real-time topographic map is a distribution map of the whole brain magnetic strength at different moments obtained according to the brain magnetic strength real-time oscillogram of the plurality of test points. The system and the method for acquiring and analyzing the magnetoencephalography data, which are provided by the atomic magnetometer, effectively overcome the defects of SQUID equipment, and the complete system from stimulation generation and magnetoencephalography acquisition to analysis is convenient to use in experiments and clinical analysis.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

Fig. 1 is a schematic structural diagram of a magnetoencephalography data acquisition and analysis system provided in an embodiment of the present application;

FIG. 2 is a schematic structural diagram of a head support according to an embodiment of the present disclosure;

FIG. 3 is a schematic structural diagram of a magnetoencephalography data acquisition and analysis based on visual stimulation according to an embodiment of the present application;

fig. 4 is a schematic flowchart of a first magnetoencephalography data acquisition and analysis method provided in an embodiment of the present application;

fig. 5 is a schematic flowchart of a second magnetoencephalography data acquisition and analysis method provided in an embodiment of the present application;

fig. 6 is a schematic flowchart of a third magnetoencephalography data acquisition and analysis method provided in an embodiment of the present application;

FIG. 7 is a real-time waveform of the magnetic brain intensity at different test points according to the embodiment of the present application;

FIG. 8 is a global real-time topographic map of the brain at various times as provided by an embodiment of the present application;

FIG. 9 is a graph of an evoked response field provided by an embodiment of the present application;

fig. 10 is a brain function network provided in an embodiment of the present application;

fig. 11 is a cause effect network provided by an embodiment of the present application;

fig. 12 is a schematic flowchart of a fourth magnetoencephalography data collection and analysis method provided in an embodiment of the present application;

fig. 13 is a diagram of a brain stimulation spectrum provided in an embodiment of the present application;

fig. 14 is a schematic flow chart of a fifth magnetoencephalography data acquisition and analysis method according to an embodiment of the present application.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

In the description of the present application, it should be noted that if the terms "upper", "lower", etc. are used for indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings or the orientation or positional relationship which is usually arranged when the product of the application is used, the description is only for convenience of describing the application and simplifying the description, but the indication or suggestion that the referred device or element must have a specific orientation, be constructed in a specific orientation and operation, and thus, cannot be understood as the limitation of the application.

Furthermore, the terms "first," "second," and the like in the description and in the claims, as well as in the drawings, are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those illustrated or described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

It should be noted that the features of the embodiments of the present application may be combined with each other without conflict.

The magnetoencephalography data acquisition and analysis method provided by the embodiment of the present application is applied to a magnetoencephalography data acquisition and analysis system based on an atomic magnetometer, and fig. 1 is a schematic structural diagram of the magnetoencephalography data acquisition and analysis system provided by the embodiment of the present application, and as shown in fig. 1, the system includes: the brain magnetic data acquisition device 20 is connected with the brain magnetic data analysis device 30, and the acquisition generation device 10 and the brain magnetic data acquisition device 20 act on the testee.

Specifically, the stimulus generating device 10 is configured to generate a stimulus and act on a subject, the magnetoencephalography data acquisition device 20 is generally worn on the brain of the subject in the form of a magnetoencephalography cap, the magnetoencephalography cap includes a plurality of atomic magnetometer probes, a contact point of each atomic magnetometer probe and the brain of the subject is used as a test point, and each atomic magnetometer is configured to acquire the magnetoencephalography intensity of each test point after the subject is stimulated by the stimulus generating device 10.

For example, the magnetic brain cap is a rigid or flexible magnetic brain cap, the magnetic brain cap has a preset distance, generally about 2mm, from the voting of the subject, and fig. 2 is a schematic structural diagram of a head support according to an embodiment of the present application, and the head of the subject can be fixed on the head support 202, so that the subject can maintain a comfortable state during the testing process.

Each atomic magnetometer probe is connected with the magnetoencephalography data analysis device 30 through a data bus to form a magnetometer channel, acquired magnetoencephalography intensity data of each test point are sent to the magnetoencephalography data analysis device 30 through each magnetometer channel, a real-time acquisition display module of the magnetoencephalography data analysis device 30 is used for analyzing the magnetoencephalography data, namely the magnetoencephalography intensity of the test points, and various magnetoencephalography analysis results are generated, wherein the various magnetoencephalography analysis results comprise at least one of the following: the brain magnetic strength real-time oscillogram and the whole brain real-time topographic map of the plurality of test points, wherein the whole brain real-time topographic map is a distribution map of the whole brain magnetic strength at different moments obtained according to the brain magnetic strength real-time oscillogram of the plurality of test points.

The real-time magnetoencephalography is used for representing the real-time change condition of the magnetoencephalography of each test point acquired by each atomic magnetometer probe after a test subject is stimulated, and the real-time magnetoencephalography is a distribution diagram obtained by calculating the magnetoencephalography of the whole brain at each moment according to the magnetoencephalography of a plurality of test points and drawing.

Furthermore, the system for acquiring and analyzing magnetoencephalography data provided in the embodiment of the present application mainly performs magnetoencephalography analysis on visual stimuli, and fig. 3 is a schematic structural diagram of the system for acquiring and analyzing magnetoencephalography data based on visual stimuli provided in the embodiment of the present application, and as shown in fig. 3, the stimulus generating device 10 includes: the device comprises a visual stimulus generating unit 101 and a display unit 102, wherein the stimulus generating unit 101 is used for generating various types of visual stimuli and sending the visual stimuli to the display unit 102 for display, the display unit 102 and a subject are co-located in a magnetic shielding cabin 201, and the subject sits, lies or stands in a secondary shielding cabin and faces the display unit 102 to receive the stimuli from the display unit 102. It should be noted that, in addition to displaying the stimulation content, the display unit 102 is also used for displaying the stimulation presentation parameters, which are parameters related to the presentation of the stimulation on the display unit 102, such as stimulation brightness, stimulation display frequency, and the like, and are used for analyzing the influence of the stimulation presentation parameters on the magnetic brain data of the subject.

The stimulus content may include, for example: the images with stimulation, such as face images with different moods, natural object images, geometric shape images, color blocks with different colors, etc., are not limited in the present application.

It should be noted that, before the test is started, the display unit 102 displays a test guide to indicate the subject to remain still or respond, and if the guide interferes with the magnetoencephalography data of the subject, the magnetoencephalography data of the guide appearing time needs to be filtered before the magnetoencephalography data analysis, so as to avoid interference.

In the embodiment of the present application, the visual stimulation generation unit 101 and the magnetoencephalography data analysis device 30 are both computer devices.

On the basis of the above embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis system, as shown in fig. 1 and fig. 3, the magnetoencephalography data acquisition and analysis system further includes: the analysis result report generating device 40 is connected with the data export module of the magnetoencephalography data analysis device 30 through a data export interface so as to receive the magnetoencephalography analysis result and score the magnetoencephalography analysis result to obtain a brain test score; the analysis result report generating means 40 may also import the questionnaire test score of the subject, and perform monitoring analysis on the subject based on the brain test score and the questionnaire test analysis to obtain the health analysis result.

On the basis of the magnetoencephalography data acquisition and analysis system, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, which is applied to the magnetoencephalography data acquisition and analysis system in fig. 1, and fig. 4 is a schematic flow diagram of a first magnetoencephalography data acquisition and analysis method provided by the embodiment of the present application, and as shown in fig. 4, the method includes:

s10: the brain magnetic data acquisition device acquires brain magnetic data generated by a subject under the stimulation of the stimulation generation device.

Specifically, the stimulus generating device 10 is configured to generate a stimulus and act on a subject, the subject generates a magnetoencephalography signal in a brain after receiving the stimulus, the magnetoencephalography data acquisition device 20 using an atomic magnetometer extracts a weak magnetoencephalography signal of each test point to obtain magnetoencephalography data, and the magnetoencephalography data includes: the magnetoencephalography intensity of a plurality of test points.

S20: the magnetoencephalography data analysis device receives the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device, analyzes the magnetoencephalography data and generates a plurality of magnetoencephalography analysis results.

Specifically, the magnetoencephalography data analysis device 30 and the magnetoencephalography data acquisition device 20 are connected through a data bus to receive magnetoencephalography data transmitted by the magnetoencephalography data acquisition device, and carry out magnetoencephalography analysis according to different magnetoencephalography analysis algorithms to generate a plurality of magnetoencephalography analysis results.

The plurality of brain magnetic analysis results include at least one of: the brain magnetic strength real-time oscillogram and the whole brain real-time topographic map of the plurality of test points, wherein the whole brain real-time topographic map is a distribution map of the whole brain magnetic strength at different moments obtained according to the brain magnetic strength real-time oscillogram of the plurality of test points. The real-time magnetoencephalography is used for representing the real-time change condition of the magnetoencephalography of each test point acquired by each atomic magnetometer probe after a test subject is stimulated, and the real-time magnetoencephalography is a distribution diagram obtained by calculating the magnetoencephalography of the whole brain at each moment according to the magnetoencephalography of a plurality of test points and drawing.

The embodiment of the application provides a magnetoencephalography data acquisition and analysis method, which is applied to a magnetoencephalography data acquisition and analysis system based on an atomic magnetometer, and the system comprises: the brain magnetic data acquisition device is connected with the brain magnetic data analysis device, and the stimulation generation device and the brain magnetic data acquisition device both act on a testee, and the method comprises the following steps: the magnetoencephalography data acquisition device acquires magnetoencephalography data generated by a testee under the stimulation of the stimulation generation device, and the magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points; the magnetoencephalography data analysis device receives the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device, analyzes the magnetoencephalography data and generates a plurality of magnetoencephalography analysis results, and the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic strength real-time oscillogram and the whole brain real-time topographic map of the plurality of test points, wherein the whole brain real-time topographic map is a distribution map of the whole brain magnetic strength at different moments obtained according to the brain magnetic strength real-time oscillogram of the plurality of test points. The method for acquiring and analyzing the magnetoencephalography data provided by the atomic magnetometer is flexible to use, does not need cooling and heat insulation, can enable the probe to be closer to the head of a testee through adjustment, enables the quality of the obtained magnetoencephalography signal to be higher, effectively overcomes the defects of SQUID equipment, is a complete set of complete method from stimulation generation and magnetoencephalography acquisition to magnetoencephalography analysis, and is convenient to use in experiments and clinical analysis.

On the basis of the foregoing embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, and fig. 5 is a schematic flow chart of a second magnetoencephalography data acquisition and analysis method provided in the embodiment of the present application, as shown in fig. 5, if the magnetoencephalography data acquisition and analysis system further includes: an analysis result report generating device, the method comprising:

s30: the analysis result report generation means imports the questionnaire test results of the subject and receives the magnetoencephalography analysis results.

Specifically, the questionnaire score is a result obtained by performing a questionnaire test on the subject before performing the brain magnetic stimulation test, and the questionnaire test and the brain magnetic stimulation test are the same in terms of test items, for example, diagnosis of a certain nervous system disease is performed on the subject. The analysis result report generation device 40 imports the questionnaire test results of the subject and receives the magnetoencephalography analysis results transmitted from the magnetoencephalography data analysis device 30.

It should be noted that the magnetoencephalography data analysis device 30 and the analysis result report generation device 40 may be integrated in the same computer device, and are two different data processing and analyzing modules in the same computer device, and the magnetoencephalography data analysis device 30 may directly send a plurality of magnetoencephalography analysis results to the analysis result report generation device 40; of course, the two different computer devices may be integrated, and the two computer devices are connected in communication, so that the computer device in which the magnetoencephalography data analysis device 30 is located transmits the plurality of magnetoencephalography results to the computer device in which the analysis result report generation device is located.

S40: and the analysis result report generating device performs health analysis on the testee according to the questionnaire test result and the magnetoencephalography analysis result to obtain a health analysis result.

Specifically, the brain magnetic analysis result only indicates the change of the magnetic field of the brain of the subject after the brain of the subject is stimulated, and cannot indicate the health condition of the subject; the questionnaire test result is a subjective judgment result obtained by performing a disease-related question and answer test on the subject, and the result depends on the experience of a doctor to some extent, so that the subject can be subjected to health analysis by combining the magnetoencephalography analysis result and the questionnaire test result to obtain a health analysis result.

Optionally, the analysis result report generating device performs comprehensive comparison on the questionnaire test result and the magnetoencephalography analysis result, and determines the correlation between the questionnaire test result and the magnetoencephalography analysis result, so as to determine the relationship between the questionnaire test result and the physiological brain information of the subject. For example, the questionnaire test result indicates that there may be some abnormal condition in the brain of the subject, and the brain magnetic field change after the subject is stimulated can be determined from the brain magnetic analysis result, and it can be determined that there is an abnormal brain health condition of the subject.

It should be noted that the analysis result report generation device may also store the health analysis result as a pdf or a word document, or may select to view an electronic version or print.

In the magnetoencephalography data acquisition and analysis method provided in the embodiment of the present application, the magnetoencephalography data acquisition and analysis system further includes: and the analysis result report generating device imports the questionnaire test result of the subject and receives the magnetoencephalography analysis result, and the analysis result report generating device performs health analysis on the subject according to the questionnaire test result and the magnetoencephalography analysis result to obtain a health analysis result. According to the embodiment of the application, the health of the testee is comprehensively analyzed by combining the questionnaire test result and the magnetoencephalography analysis result of the tester, so that the universal applicability of the magnetoencephalography data acquisition and analysis method in clinical diagnosis is improved.

On the basis of the foregoing embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, fig. 6 is a schematic flow chart of a third magnetoencephalography data acquisition and analysis method provided in the embodiment of the present application, and as shown in fig. 6, the foregoing S40 includes:

s41: matching the questionnaire test results of the same type with the magnetoencephalography analysis results to obtain matching degree parameters.

Specifically, when a questionnaire test for a certain disease is performed on a subject, the design of the questionnaire test is often considered from multiple dimensions, that is, multiple causes of the certain disease are considered, and multiple magnetoencephalography results are also multiple dimensions, and in addition to the magnetoencephalography and the whole brain real-time topography, the magnetoencephalography results further include: the frequency of activity of the brain after stimulation, the energy of brain activity at the frequency of activity, the peak in magnetic field strength that induces a response in the brain, and the response time corresponding to the peak in magnetic field strength.

Optionally, the questionnaire test results of the same type and the results of the magnetoencephalography analysis may be matched, and a matching degree parameter is calculated, for example, the results of the questionnaire test results on the response speed of the tested brain and the response time corresponding to the peak value of the magnetic field strength are matched; or matching the response result of the questionnaire test result to a certain visual stimulus with the activity frequency and the brain activity intensity of the brain subjected to the same visual stimulus, and the like, and performing matching analysis according to the needs of experiments or clinical diagnosis.

The matching degree parameter, that is, the correlation between the questionnaire test result and the magnetoencephalography result, may be calculated by using statistical correlation analysis methods such as Spearman correlation and Pearson correlation, which is not limited in the present application. The matching degree parameter can be expressed in percentage form to embody the matching degree.

S42: and acquiring a health analysis result according to the matching degree parameter.

Specifically, the relationship between the cognitive behavior of the subject and the brain physiological signal can be analyzed by calculating the matching degree parameter, and the health analysis result is displayed in a text or table form, which at least comprises: matching degree of the questionnaire test results and the brain magnetic analysis results of the same type, and a conclusion generated based on the matching degree, wherein the conclusion can be that the function of a certain brain area of the subject is abnormal, and the like. For example, the result of testing the brain response speed and the matching result of the response time corresponding to the magnetic field strength peak can judge the brain response speed of the subject; in the questionnaire test result, the response result of the subject to a certain visual stimulus and the matching result of the activity frequency and the brain activity intensity of the brain subjected to the same visual stimulus can be used for analyzing the brain region and the like influencing the subject to respond to the cognitive behavior under the visual stimulus.

According to the magnetoencephalography data acquisition and analysis method provided by the embodiment of the application, the questionnaire test results and the magnetoencephalography analysis results of the same type are matched to obtain the matching degree parameters, and the health analysis results are obtained according to the matching degree parameters. According to the embodiment of the application, the health analysis result is obtained through the memorability matching calculation of the questionnaire test result and the magnetoencephalography analysis result of the same type, so that the accuracy of the analysis result is improved in the experiment or clinical diagnosis.

On the basis of the foregoing embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, where the S20 includes:

the magnetoencephalography data analysis device calculates the magnetoencephalography intensity of the test points to obtain the magnetoencephalography intensities of a plurality of brain areas of the whole brain, and the magnetoencephalography intensities of the brain areas are used for generating a real-time topographic map of the whole brain.

Specifically, the magnetoencephalography data acquisition device 20 can only acquire the magnetoencephalography intensity of a test point, and to acquire the magnetoencephalography intensities of a plurality of brain areas of the whole brain, the magnetoencephalography intensities of different brain areas are calculated according to the change conditions of the magnetoencephalography signals in the whole brain, and the magnetoencephalography intensities of different areas of the cranium of the subject are expressed by different colors. For example, fig. 7 is a real-time waveform diagram of the magnetic brain intensity at different test points provided by the embodiment of the present application, and fig. 8 is a real-time topographic map of the whole brain at different times provided by the embodiment of the present application.

According to the magnetoencephalography data acquisition and analysis method provided by the embodiment of the application, the magnetoencephalography data analysis device calculates the magnetoencephalography intensity of a plurality of test points to obtain the magnetoencephalography intensity of a plurality of brain areas of the whole brain, and the magnetoencephalography intensity of the plurality of brain areas is used for generating a real-time topographic map of the whole brain. The influence among all brain areas after different types of stimulation can be clearly known through the whole brain real-time topographic map, and reference is provided for researching functional association among the brain areas in experiments.

On the basis of the above embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, where the method further includes:

the magnetoencephalography data analysis device obtains a plurality of groups of magnetoencephalography data acquired by the magnetoencephalography data acquisition device based on a plurality of times of stimulation, and each group of magnetoencephalography data comprises: the magnetoencephalography intensity of a plurality of test points at different moments; calculating to obtain an evoked response field parameter of each test point according to a plurality of magnetoencephalography intensities of each test point at the same time, wherein the evoked response field parameter comprises: the magnetoencephalography intensity peak value, the peak value moment, the peak value interval and the magnetoencephalography intensity average value at the same moment of each test point; the mean value of the magnetoencephalography at the same time is plotted as the evoked response field of each test point.

Specifically, the evoked response refers to the response condition of a subject at different test points after being stimulated, and in order to determine the evoked response field parameters and the evoked response field pattern, multiple parallel experiments need to be performed on the subject, where the multiple parallel experiments refer to multiple experiments performed on the subject by using the same stimulus, and the response condition of the subject after being stimulated is determined according to the results of the multiple experiments.

The average value of the magnetic brain intensity at the same time is calculated for a plurality of magnetic brain intensities tested by each test point for a plurality of times, and the average value of the magnetic brain intensity at different times of each test point is drawn as an evoked response field map of each test point, wherein the evoked response field map is actually a magnetic brain intensity waveform map. In the calculation process, or the peak value, the peak moment and the peak interval of the magnetoencephalography of each test point can be directly obtained from the evoked response field diagram.

For example, fig. 9 is an evoked response field diagram provided by an embodiment of the present application, as shown in fig. 9, three evoked response field diagrams are waveforms of magnetic brain intensity after three subjects of different ages are subjected to the same stimulus for multiple times, and a solid line and a dotted line represent waveforms of magnetic brain intensity corresponding to two different types of stimuli.

It should be noted that, for a visual stimulus, according to each evoked response field parameter, the response sequence and response time of each test point under the stimulus can be determined, which is used for diagnosing neuropathy on the visual pathway.

In the magnetoencephalography data acquisition and analysis method provided by the embodiment of the application, a magnetoencephalography data analysis device acquires multiple groups of magnetoencephalography data acquired by the magnetoencephalography data acquisition device based on multiple times of stimulation, calculates and obtains an evoked response field parameter of each test point according to multiple magnetoencephalography intensities of each test point at the same moment, and draws the mean value of the magnetoencephalography intensities at the same moment into an evoked response field map of each test point. The method and the device determine the induced response condition of the brain of the subject after stimulation through multiple times of stimulation, so that the experimental result is more accurate.

On the basis of the above embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, where the method further includes:

the magnetoencephalography data analysis device calculates the correlation of magnetoencephalography intensity of a plurality of brain areas; drawing the correlation among a plurality of brain areas on a brain scanning structure chart to obtain a brain function network; determining the connection strength between any two brain areas according to the correlation between a plurality of brain areas in the brain function network.

Specifically, after the brain is stimulated and generates a brain magnetic signal, the brain magnetic signal can be transmitted among a plurality of brain areas, so that the brain magnetic signal can be generated by the plurality of brain areas, the correlation of the brain magnetic strength among different brain areas is calculated according to the brain magnetic strength of the plurality of brain areas of the whole brain, the correlation can represent the influence between any two brain areas after the brain is stimulated, and whether the function of one brain area can influence the function of the other brain area can be analyzed. The brain scanning structure chart of the subject is obtained in advance, and the correlation among a plurality of brain areas is drawn on the brain scanning structure chart to obtain a brain function network. The correlation between multiple brain regions is expressed in terms of the connection strength, and the higher the correlation, the stronger the connection strength.

Fig. 10 is an exemplary brain function network provided in an embodiment of the present application, and as shown in fig. 10, thicknesses of connection lines of different brain regions indicate connection strengths between the brain regions, and fig. 10 shows brain function network diagrams from three directions of a left view, a top view and a right view of a brain scan structure diagram.

According to the magnetoencephalography data acquisition and analysis method provided by the embodiment of the application, a magnetoencephalography data analysis device calculates the correlation of magnetoencephalography intensities of a plurality of brain areas; drawing the correlation among a plurality of brain areas on a brain scanning structure chart to obtain a brain function network; determining the connection strength between any two brain areas according to the correlation between a plurality of brain areas in the brain function network. By calculating the correlation and connection strength between multiple brain regions, the relationship between brain regions can be analyzed, facilitating the determination of brain regions that may be affected by disease in a clinical setting.

On the basis of the above embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, where the method further includes:

the magnetoencephalography data analysis device calculates the stimulation divergence direction and the stimulation divergence intensity of the magnetoencephalography intensity of a plurality of brain areas; drawing stimulation divergence directions and stimulation divergence strengths of a plurality of brain areas on a brain scanning structure chart to obtain an effect network; and determining the activity between any two brain areas according to the stimulation divergence intensity of a plurality of brain areas in the factor network.

Specifically, before the divergent directions of stimulation of a plurality of brain areas are determined, the brain area which firstly responds after being stimulated needs to be determined, namely, the brain magnetic source positioning is performed, and the method for positioning the brain magnetic source comprises the following steps:

the brain magnetic data analysis system acquires a brain scanning structure chart of a subject, and projects magnetic signals of a plurality of test points to the interior of a brain in the brain scanning structure chart according to the positions of the test points relative to the brain of the subject to obtain a brain magnetic signal chart; and positioning the magnetoencephalography according to the magnetoencephalography and the whole brain real-time topographic map. The source positioning result can be displayed in the structure chart of the magnetoencephalography according to the magnetoencephalography source positioning result.

After the brain magnetic source is positioned to determine the brain area which is firstly responded by the stimulation, the direction of the stimulation divergence from the brain area to other brain areas is determined, the stimulation divergence intensity is calculated, and the stimulation divergence direction and the stimulation divergence intensity of a plurality of brain areas are drawn on a brain scanning structure chart to obtain the cause-effect network. The greater the intensity of stimulus divergence, the greater the activity between brain regions. Of course, a significantly active brain region connection path may also be determined by the causal network.

For example, fig. 11 is a cause-effect network provided by an embodiment of the present application, as shown in fig. 11, arrows indicate directions of divergence of stimulation, the left cause-effect network indicates that there are multiple brain regions as source brain regions after stimulation, and the right cause-effect network indicates that only one brain region as a source locates a brain region after stimulation.

According to the magnetoencephalography data acquisition and analysis method provided by the embodiment of the application, a magnetoencephalography data analysis device calculates stimulation divergence directions and stimulation divergence strengths of magnetoencephalography strengths of a plurality of brain areas; drawing stimulation divergence directions and stimulation divergence strengths of a plurality of brain areas on a brain scanning structure chart to obtain an effect network; and determining the activity between any two brain areas according to the stimulation divergence intensity of a plurality of brain areas in the factor network. By calculating the stimulation divergence direction and the stimulation divergence intensity among a plurality of brain regions, the relationship among the brain regions can be analyzed, and the brain regions possibly affected by diseases can be conveniently determined in clinical aiming.

On the basis of the foregoing embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, where the magnetoencephalography data acquisition and analysis system further includes: eye movement detection means and motion detection means; fig. 12 is a schematic flowchart of a fourth magnetoencephalography data acquisition and analysis method provided in an embodiment of the present application, and as shown in fig. 12, the method further includes:

s50: eye movement data of the subject is detected by an eye movement detection device.

Specifically, the eye movement detection device is arranged opposite to the testee to monitor the eye movement condition of the testee in real time, and the eye movement detection device is connected with the magnetoencephalography data analysis device to send the eye movement data to the magnetoencephalography data analysis device.

The eye movement detecting device is an eye movement detector, and may be an image pickup apparatus having an eye movement recognition function, for example.

S60: motion data of the subject is detected by a motion detection device.

Specifically, the motion detection device is used for detecting the motion condition of the brain of the subject in the test process, the operation detection device is arranged inside the magnetoencephalography data acquisition device, namely inside the magnetoencephalography cap, the motion data of the subject is determined by acquiring the position of the motion detection device relative to the brain in the test process, and the motion detection device is connected with the magnetoencephalography data analysis device so as to send the motion data to the magnetoencephalography data analysis device.

For example, the motion detection device may be a plurality of position sensors disposed at landmark points inside the magnetoencephalophone cap.

Before S20, the method further includes:

s70: the magnetoencephalography data analysis device denoises the magnetoencephalography data according to the eye movement data and the motion data.

Specifically, after the magnetoencephalography data analysis device acquires the eye movement data, the direction in which the eyes of the subject look during the test process, namely the gaze direction, or the movement track of the eyeballs of the subject within a period of time, namely the saccade direction, can be determined, the gaze direction and the saccade direction are common research indexes of the eye movement data, and the change of the excitement of the subject can be measured through the saccade times, frequency and delay, the gaze frequency and the duration.

And calculating whether the subject watches the visual stimulation or not by utilizing the track information of the eye movement data so as to eliminate the magnetoencephalography signals generated when the subject does not watch the visual stimulation.

The real-time position of a subject in a coordinate system of a magnetic shielding room can be determined by utilizing the motion data, the real-time position of a helmet with a probe in the shielding room can be determined in real time according to helmet mark points, and the real-time position of each magnetometer in a coordinate system in the human brain in the experimental process is finally calculated by utilizing the external characteristics of the human body and the internal structure data of the human body. By utilizing the position change information, the noise generated by shaking of the testee in the experimental process can be calculated, and then the noise component is reduced, so that the signal-to-noise ratio of the brain magnetic signal is improved.

It should be noted that, in addition to being used for data denoising, the eye movement data can also be used for analyzing the relationship between the change of the magnetoencephalography signal and the cognitive behavior of the subject under specific stimulation through the saccade number, frequency and delay, and the fixation frequency and duration on a physiological level.

In the magnetoencephalography data acquisition and analysis method provided in the embodiment of the present application, the magnetoencephalography data acquisition and analysis system further includes: eye movement detection apparatus and motion detection apparatus, the method further comprising: detecting eye movement data of the subject by an eye movement detection device; detecting motion data of the subject by a motion detection device; the magnetoencephalography data analysis device denoises the magnetoencephalography data according to the eye movement data and the motion data. The accuracy of the magnetoencephalography analysis result can be improved by denoising the magnetoencephalography data according to the eye movement data and the motion data.

In an alternative embodiment, the frequency of activity of the brain after stimulation and the energy of brain activity at that frequency may be calculated using the following method:

the magnetoencephalography data analysis system calculates a plurality of groups of magnetoencephalography data to obtain brain activity frequency data and activity intensity data corresponding to the brain activity frequency; and (4) drawing the brain activity frequency data and the activity intensity data to obtain a brain stimulation frequency spectrogram.

Fig. 13 is a spectrum diagram of brain stimulation according to an embodiment of the present application, which shows a relationship between time, activity frequency, and activity intensity, wherein the activity intensity is represented by different colors.

It should be noted that all the above calculations of the magnetoencephalography data are performed in different brain function analysis modules in the magnetoencephalography data analysis apparatus, and the different brain function analysis modules have corresponding analysis algorithms or algorithms customized by experimenters. After different magnetoencephalography results are output, the same experimental result can be obtained from the magnetoencephalography database, and the magnetoencephalography result of the subject and the results of other healthy or sick subjects are subjected to statistical analysis; the embodiment of the application is suitable for carrying out attention-related evoked response and evoked response magnetoencephalography experiments in cognitive science, and can also be suitable for diagnosing attention-deficient autism, hyperactivity and other nerve developmental diseases.

By way of example, when performing asperger syndrome diagnosis or psychogenic disease diagnosis, the questionnaire test adopted clinically in the embodiment of the present application is a wecker scale, a Gesell development scale, and the like, which performs multiple intellectual behavior tests, and the test scale has identification of images about special shapes, articles, natural things, or emotions, and when performing clinical diagnosis for the disease, a clinician uses the test scale to score multiple intelligence and responses of a subject, then uses a visual brain magnetic experiment to present the same stimuli as those in the test scale, records brain magnetic data, calculates the activity intensity of brain magnetic frequency segments, the activity degree of brain functional regions, and the connection intensity between brain functional regions after the subject receives the stimuli, and matches and scores the database data; finally, calculating the correlation between the questionnaire test result of the test scale and the brain magnetic analysis result score, and if the correlation is strong, determining whether the patient is ill; if the correlation is weak, further diagnosis is required.

On the basis of the foregoing embodiments, an embodiment of the present application further provides a magnetoencephalography data acquisition and analysis method, which is applied to a magnetoencephalography data analysis device in a system of the embodiment, and fig. 14 is a schematic flow diagram of a fifth magnetoencephalography data acquisition and analysis method provided in the embodiment of the present application, and as shown in fig. 14, the method includes:

s21: receiving the magnetoencephalography data transmitted by the magnetoencephalography data acquisition device; wherein the magnetoencephalography data comprises: the brain magnetic strength of a plurality of test points generated by the test subject under the stimulation of the stimulation generating device.

S22: analyzing the magnetoencephalography data to generate a plurality of magnetoencephalography analysis results, wherein the plurality of magnetoencephalography analysis results comprise at least one of the following: the brain magnetic strength real-time oscillogram and the whole brain real-time topographic map of the plurality of test points, wherein the whole brain real-time topographic map is a distribution map of the whole brain magnetic strength at different moments obtained according to the brain magnetic strength real-time oscillogram of the plurality of test points.

Specific implementation processes and beneficial effects of the embodiments of the present application can be referred to above, and are not described herein in detail.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and shall be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.