阅读说明：本技术 一种有机肥发酵的方法及有机肥 (Organic fertilizer fermentation method and organic fertilizer ) 是由 阚凤玲 陈日远 马倩 李新博 刘鹏 于 2021-09-30 设计创作，主要内容包括：本申请涉及有机肥制备的技术领域,具体公开了一种有机肥发酵的方法及有机肥。一种有机肥发酵的方法：包括以下步骤,S1：起始物料的选择；S2：添加复合菌种；S3：添加发酵功能增效剂,S4：返混,发酵包括发酵初期、发酵中期和发酵后期,将发酵中期3-5天的物料进行返混,返混量为起始物料的10-30%。本申请有机肥发酵的方法,能够使起始物料快速升温,缩短发酵时间,无需除臭装置,且有机废弃物彻底降解,大大降低硫化物、氮氧化物等恶臭气体排放。有机肥内的有机物总量在69.1%以上,有机质的含量在64.9%以上,易氧化有机质的含量在26.3%以上,符合国家有机肥标准。(The application relates to the technical field of organic fertilizer preparation, and particularly discloses an organic fertilizer fermentation method and an organic fertilizer. A method for fermenting organic fertilizer comprises the following steps: comprising the following steps, S1: selecting a starting material; s2: adding a composite strain; s3: adding a fermentation function synergist, S4: and (3) back mixing, wherein the fermentation comprises a fermentation initial stage, a fermentation middle stage and a fermentation later stage, and the materials in the fermentation middle stage are back mixed for 3-5 days, and the back mixing amount is 10-30% of the initial materials. The organic fertilizer fermentation method can quickly heat up starting materials, shorten the fermentation time, avoid a deodorizing device, thoroughly degrade organic wastes, and greatly reduce emission of malodorous gases such as sulfides and nitrogen oxides. The total amount of organic matters in the organic fertilizer is more than 69.1 percent, the content of the organic matters is more than 64.9 percent, and the content of the easily-oxidized organic matters is more than 26.3 percent, thereby meeting the national organic fertilizer standard.)

1. A method for fermenting organic fertilizer is characterized by comprising the following steps,

s1: the starting material comprises the following components, by weight, 250 portions of livestock and poultry manure and 400 portions of adjusting material, and 175 portions of adjusting material and 200 portions of adjusting material;

s2: adding composite strains, wherein the adding amount of the composite strains is 0.1-1.2% of the initial material;

s3: adding a fermentation function synergist which comprises, by weight, 50-90 parts of kitchen fermented biological humic acid, 5-10 parts of quick lime, 0.01-0.1 part of polyaspartic acid, 6-12 parts of vermiculite powder, 2-5 parts of zeolite powder and 10-15 parts of a nitrification inhibitor;

s4: back mixing, wherein the fermentation comprises a fermentation initial stage, a fermentation middle stage and a fermentation later stage, the materials in the fermentation middle stage for 3-5 days are back mixed, the back mixing amount is 10-30% of the initial materials,

wherein the composite strain comprises mesophilic bacteria, thermophilic bacteria and growth-promoting bacteria.

2. The method for fermenting the organic fertilizer as claimed in claim 1, which is characterized in that: the water content of the starting material is 45-55%, and the weight ratio of carbon to nitrogen is (25-35):1, wherein the active organic carbon accounts for 20-30% of the total carbon, and the quick-acting nitrogen accounts for 30-35% of the total nitrogen.

3. The method for fermenting the organic fertilizer as claimed in claim 1, which is characterized in that: the weight ratio of the mesophilic bacteria, the thermophilic bacteria and the growth promoting bacteria is 1: (2.1-2.6):(0.8-1.2).

4. The method for fermenting the organic fertilizer as claimed in claim 3, which is characterized in that: the mesophilic bacteria are micrococcus luteus, clostridium cellulolyticum and bacillus firmus, and the weight ratio of micrococcus luteus to clostridium cellulolyticum to bacillus firmus is (2-3) to (1-2) to (1-3).

5. The method for fermenting the organic fertilizer as claimed in claim 3, which is characterized in that: the thermophilic bacteria are orange thermophilic ascomycetes.

6. The method for fermenting the organic fertilizer as claimed in claim 3, which is characterized in that: the growth-promoting bacteria are azospirillum, alcaligenes and mesorhizobium, and the weight ratio of azospirillum, alcaligenes and mesorhizobium is 1 (1-1.2) to 1-1.6.

7. The method for fermenting the organic fertilizer as claimed in claim 1, which is characterized in that: the fermentation function synergist also comprises 0.01-0.02 part of alpha-naphthylacetic acid.

8. The method for fermenting the organic fertilizer as claimed in claim 1, which is characterized in that: the nitrification inhibitor is one or more of 2-chloro-6- (trichlorotoluene) pyridine, guanyl thiourea (ASU) and dicyandiamide (DCD).

9. An organic fertilizer is characterized in that: prepared by the method of fermenting an organic fertilizer as claimed in any one of claims 1 to 8.

10. The organic fertilizer according to claim 9, characterized in that: the total organic matter content of the organic fertilizer is more than 69.1%, the organic matter content is more than 64.9%, and the content of the easily-oxidized organic matter is more than 26.3%.

Technical Field

The application relates to the technical field of organic fertilizer preparation, in particular to an organic fertilizer fermentation method and an organic fertilizer.

Background

The main required nutrients of the plants in the growth process are elements such as nitrogen, phosphorus and potassium, and the soil can cause nutrient loss in the long-term planting process, so that the fertilizer is applied to the land, namely, the nitrogen, phosphorus and potassium elements are supplemented to the land, and the yield of crops is increased. In the planting process, chemical fertilizers play a very important role, but with the frequent and single use of the fertilizers, the soil environment is also adversely affected. Under the condition that the soil is lack of sufficient organic matters, the granular structure of the soil is difficult to form, the adhesion between the soils is difficult to form, and then the water retention capacity of the soil is gradually reduced. In order to solve the problem, the organic fertilizer is produced at the same time.

The organic fertilizer is mainly derived from plants and/or animals, and is a carbon-containing material which is applied to soil to provide plant nutrition as a main function. The fertilizer is prepared from biological substances, animal and plant wastes and plant residues, eliminates toxic and harmful substances in the fertilizer, and is rich in a large amount of beneficial substances including various organic acids, peptides and rich nutrient elements including nitrogen, phosphorus and potassium. The organic fertilizer not only can provide comprehensive nutrition for crops, but also has long fertilizer efficiency, can increase and update soil organic matters, promote microbial propagation, improve the physical and chemical properties and biological activity of soil, and is a main nutrient for green food production.

At present, the organic fertilizer is mainly prepared by fermentation. However, the existing preparation method has the problems of slow temperature rise in the fermentation process, overlong fermentation time (the fermentation time is more than 27 days), big odor and the like. Finally, the content of organic matters and organic matters in the organic fertilizer is low, and the organic fertilizer cannot meet the requirements of national commercial organic fertilizer standards.

Disclosure of Invention

In order to solve the problems of slow temperature rise, long fermentation time and large odor of the organic fertilizer in the fermentation process, the application provides the organic fertilizer fermentation method and the organic fertilizer, and the content of organic matters and organic matters in the organic fertilizer is increased.

In a first aspect, the application provides a method for fermenting an organic fertilizer, which adopts the following technical scheme:

a method for fermenting organic fertilizer comprises the following steps,

s1: the starting material comprises the following components, by weight, 250 portions of livestock and poultry manure and 400 portions of adjusting material, and 175 portions of adjusting material and 200 portions of adjusting material;

s2: adding composite strains, wherein the adding amount of the composite strains is 0.1-1.2% of the initial material;

s3: adding a fermentation function synergist which comprises, by weight, 50-90 parts of kitchen fermented biological humic acid, 5-10 parts of quick lime, 0.01-0.1 part of polyaspartic acid, 6-12 parts of vermiculite powder, 2-5 parts of zeolite powder and 10-15 parts of a nitrification inhibitor;

s4: back mixing, wherein the fermentation comprises a fermentation initial stage, a fermentation middle stage and a fermentation later stage, the materials in the fermentation middle stage for 3-5 days are back mixed, the back mixing amount is 10-30% of the initial materials,

wherein the composite strain comprises mesophilic bacteria, thermophilic bacteria and growth-promoting bacteria.

Preferably, the livestock manure comprises one of pig manure, cow manure and chicken manure.

Preferably, the adjusting material comprises peanut shells, corn straws and cotton stalks.

Preferably, the weight ratio of carbon to nitrogen in the starting material is (25-35):1, the water content is 45-55%, wherein the active organic carbon accounts for 20-30% of the total carbon, and the quick-acting nitrogen accounts for 30-35% of the total nitrogen.

By adopting the technical scheme, when the pig manure is selected as the livestock manure, the water content in the initial material is generally 45-55%; when the chicken manure is used, the water content in the starting material is generally 45-50%; when cow dung is used, the water content in the starting material is 50-55%. The adjusting material in the starting material is plant straw in daily life, generally selected corn straw which is relatively wide and is easy to obtain. Preferably, the corn stalks need to be crushed, so that the fermentation time can be shortened.

The starting material is added with the compound strain for fermentation, and the compound strain needs a proper environment for functioning, namely a proper carbon-nitrogen ratio. The weight ratio of carbon to nitrogen in the starting material is (25-35):1, wherein the active organic carbon accounts for 20-30% of the total carbon, and the quick-acting nitrogen accounts for 30-35% of the total nitrogen. The active organic carbon and the quick-acting nitrogen can be quickly utilized by the composite strain, when the content of the active organic carbon and the quick-acting nitrogen is low, the composite strain cannot be quickly propagated, and the temperature of the organic fertilizer cannot be increased or is slowly increased; when the content of the active organic carbon and the quick-acting nitrogen is too high, the active organic carbon and the quick-acting nitrogen are quickly degraded by the composite strain, so that the temperature in the starting material is too high, and the yield is reduced.

The composite strain and the fermentation function synergist are added into the starting material, and the mutual cooperation and mutual promotion of the composite strain and the fermentation function synergist can quickly heat the starting material and shorten the fermentation time; the content of organic matters in the fermented organic fertilizer is ensured, and the emission of malodorous gases such as sulfide and nitrogen oxide is greatly reduced. Finally, the initial materials in the middle stage of fermentation (temperature is 60 ℃) within 3-5 days are back-mixed through back-mixing operation, the back-mixing amount is 10-30%, and the optimal effect is 20%.

As shown in the later embodiment, the pig manure and the corn straw are mixed to form a starting material, wherein the water content of the starting material is 50%, the ratio of carbon to nitrogen is 25:1, active organic carbon accounts for 25% of total carbon, and quick-acting nitrogen accounts for 30% of total nitrogen; the starting materials in the middle stage of fermentation (temperature up to 60 ℃) within 4 days are back-mixed, and the back-mixing amount is 20%.

Quinone-based substances are generated in the fermentation process of the starting materials, and the quinone-based substances are used as important prepolymers and electron shuttles formed by humic acid and are important substances formed by the humic acid. The quinone-based substance and the small molecular substance (such as amino acid) can form humic acid to accelerate the humic process. However, quinone-based substances are generally produced in the middle and later stages of fermentation, and small molecular substances are mostly produced in the early stages of fermentation. Therefore, the small molecular substances are easy to mineralize and lose, odor is released, once the odor is lost, the odor cannot enter the next intermediate link, and the problem of low organic matter content in the organic fertilizer is caused. In order to solve the problem, biological humic acid fermented by kitchen is added in the application, functional groups such as quinone group and hydroxyl group in the humic acid participate in redox reaction, and the quinone group and small molecules (such as amino acid) in the pre-fermentation period can also form humic acid; carbon dioxide and water, which are indispensable substances in plant life activities, are also generated at the same time. The kitchen fermented biological humic acid is used as a fermentation promoter, on one hand, small molecular substances can be fixed through adsorption or chelation and the like, and on the other hand, the biological fulvic acid can promote the growth and the propagation of microorganisms.

The composite bacteria are added in the application, and can promote the generation of a small amount of quinone-based substances in the early stage of fermentation, mainly promote the generation of the quinone-based substances in the middle stage of fermentation. And then the back mixing operation in the application is matched, the fermentation product with the quinone-based substances in the middle stage of fermentation is back mixed to the initial stage of fermentation, so that the untreated or reacted micromolecule substances can be reused, and the mineralization quantity of the micromolecule substances is greatly reduced. The addition of the compound bacteria enables the fermentation process of the starting material to discharge less odor, maximally fixes carbon and nitrogen in the starting material, and further maximizes the content of organic matters in the fermented organic fertilizer.

Preferably, the composite strain comprises mesophilic bacteria, thermophilic bacteria and growth-promoting bacteria, and the proportion of the mesophilic bacteria, the thermophilic bacteria and the growth-promoting bacteria is 1: (2.1-2.6):(0.8-1.2).

By adopting the technical scheme, the fermentation principle of the method is that aerobic bacteria are taken as main materials to oxidize, absorb and decompose initial materials. Microorganisms involved in the degradation of organic matter include two types, mesophilic bacteria (i.e., mesophilic bacteria) and thermophilic bacteria (i.e., thermophilic bacteria). The fermentation process of the starting material can be divided into three stages: in the initial stage of fermentation, the initial temperature of the starting material is generally 15-25 deg.C, and after inoculation of mesophilic bacteria, the mesophilic bacteria are relatively active, and soluble substances such as saccharide and starch in the starting material can be utilized to rapidly propagate, so as to gradually raise the temperature in the starting material. When the temperature in the starting material rises to above 45 ℃, the fermentation of the starting material begins. The temperature in the starting material can reach 65-70 ℃ or higher within 24 h. When the temperature exceeds 45 ℃, mesophilic bacteria die gradually, thermophilic fungi and bacteria are activated gradually, soluble organic matters formed in the decomposition process in the initial fermentation stage are decomposed continuously, and complex organic matters such as hemicellulose, cellulose, protein and the like are decomposed intensively. After the middle fermentation period is finished, the temperature is reduced to be below 40 ℃, the fermentation period is carried out, the second inoculation is carried out, the growth-promoting bacteria are inoculated, the growth-promoting bacteria are rapidly propagated and grown in the initial material in a large amount, the produced organic fertilizer has the function of a biological organic fertilizer, the soil nutrients and organic matters can be increased, the soil micro-ecosystem can be improved, the occurrence of soil-borne diseases is reduced, and the continuous cropping obstacle is solved.

Preferably, the mesophilic bacteria are micrococcus luteus, clostridium cellulolyticum and bacillus firmus, and the weight ratio of the micrococcus luteus to the clostridium cellulolyticum to the bacillus firmus is (2-3) to (1-2) to (1-3).

By adopting the technical scheme, the mesophilic bacteria is the combination of micrococcus luteus, clostridium cellulolyticum and bacillus firmus, and is mixed according to the weight ratio of (2-3) to (1-2) to (1-3). The effective number of mesophilic bacteria is not less than 10⁶cfu/g。

The components in the starting material are complex, the nutrient substances are diversified, and the using effect of a single strain is poor. However, when different types or attributes of strains are mixed together, antagonism can be generated among certain strains, and the aims of synergy and optimized fermentation and decay are not achieved at all. In the application, through screening, the combination of micrococcus luteus, clostridium cellulolyticum and bacillus firmus can not generate antagonistic action. And the mesophilic bacteria effectively utilize soluble substances in the starting material, such as sugar, starch and the like to rapidly propagate under the combination of a specific proportion, so that the temperature in the starting material is rapidly increased, and the aims of rapidly increasing the temperature and shortening the fermentation time are fulfilled.

Preferably, the thermophilic bacterium is thermoascus aurantiacus.

Preferably, veratryl alcohol inducer and surfactant are added to thermoascus aurantiacus.

Preferably, the veratryl alcohol inducer is 0.1-0.25% by weight of thermoascus aurantiacus, and the surfactant is 0.2-0.43% by weight of thermoascus aurantiacus.

By adopting the technical scheme, the thermophilic ascomycetes has good high temperature resistance and high humidity resistance. When the temperature in the starting material rises to above 45 ℃, the fermentation enters the middle stage of fermentation, at the moment, the thermoascus aurantiacus gradually starts to be active, the fermentation starts, and the temperature in the starting material can reach 65-70 ℃ or higher within about 7-9 days. In the middle stage of fermentation, the soluble organic matters remained and formed in the decomposition process in the previous stage are continuously decomposed, and complex organic matters such as hemicellulose, cellulose, protein and the like are intensively decomposed.

The addition of the inducer and the surfactant can improve the capability of thermophilic bacteria to generate degrading enzymes, thereby improving the degradation effect. The quinophthalol is a benzene ring derivative with lignin structural characteristics, is used as an intermediate for synthesizing the ligninase, and can well play a role in inducing and generating the ligninolytic enzyme. The surfactant is preferably Tween 80, and the surfactant can reduce the inhibition effect caused by intracellular accumulation of synthesized degrading enzyme mainly by improving the permeability of cell membrane.

Preferably, the growth-promoting bacteria are azospirillum, alcaligenes and Mesorhizobium, and the proportion of the azospirillum, the alcaligenes and the Mesorhizobium is 1 (1-1.2) to 1-1.6.

By adopting the technical scheme, the growth-promoting bacteria added at the later stage of fermentation comprise azospirillum, alcaligenes and Mesorhizobium. The growth-promoting bacteria can convert organic matters in soil into inorganic matters, and provide effective mineral nutrition for plants; meanwhile, the growth-promoting bacteria can also secrete indoleacetic acid, vitamins and antibiotics, and 1-aminocyclopropane-1-carboxylic acid is used as a unique nitrogen source to directly or indirectly promote the growth of plants.

As shown in the following embodiment, the mesophilic bacteria, thermophilic bacteria and growth-promoting bacteria are added in a weight ratio of 1:2.4:1, wherein the weight ratio of micrococcus luteus, clostridium cellulolyticum and bacillus firmus in the mesophilic bacteria is 2:2:3, and the weight ratio of azospirillum, alcaligenes and mesorhizobium in the growth-promoting bacteria is 1:1: 1.5. Under the weight ratio, mesophilic bacteria, thermophilic bacteria and growth-promoting bacteria can all play a role well, the heating speed of starting materials can be increased, emission of malodorous gases such as sulfide and nitrogen oxide is reduced, and the content of organic matters and organic matters in the organic fertilizer is improved.

Preferably, the fermentation function synergist also comprises 0.01-0.02 part of alpha-naphthylacetic acid.

By adopting the technical scheme, the starting raw materials contain cellulose, lignin and the like which are difficult to degrade, so that the decomposition process is influenced, and the waste of the biomass energy is further caused. The alpha-naphthylacetic acid is supplemented in the initial fermentation stage of the starting material, so that the degradation of organic matters which are difficult to degrade in the starting material by the composite strain can be promoted, the process of the decomposition and the planting is accelerated, the temperature in the starting material can reach the peak value more quickly, the fermentation time is shortened, and the production cost is saved.

Preferably, the nitrification inhibitor is one or more of 2-chloro-6- (trichlorotoluene) pyridine, guanylthiourea (ASU), dicyandiamide (DCD).

By adopting the technical scheme, the nitrification inhibitor can reduce the loss of the nitrogen fertilizer, improve the utilization rate of the nitrogen fertilizer, reduce the content of nitrite in crops, improve the quality of the crops and reduce the pollution to soil, underground water and environment due to over-high application. And growth-promoting bacteria are matched, so that the fermented starting material has rich organic matters and provides nutrient elements required in the growth process of plants.

In a second aspect, the application provides an organic fertilizer, which adopts the following technical scheme:

an organic fertilizer is prepared by the organic fertilizer fermentation method.

Preferably, the total organic matter content of the organic fertilizer is greater than 69.1%, the organic matter content is greater than 64.9%, and the content of the easily-oxidized organic matter is greater than 26.3%.

By adopting the technical scheme, the starting material with the water content and the carbon-nitrogen ratio meeting the requirements is selected as the fermentation raw material for fermentation. The composite strain and the fermentation function synergist are added into the starting material, and the combination of the two added components enables the starting material to be heated rapidly for fermentation, so that the fermentation time is shortened. The odor emission is reduced in the fermentation process, and the back mixing operation is matched, so that the organic matter content in the finally prepared organic fertilizer is more. The organic fertilizer is applied to the plant growth process, can promote the plant growth, increase the yield and reduce the pollution to soil and environment.

In summary, the present application has the following beneficial effects:

1. because the method of starting materials, compound strains, fermentation function synergist and back mixing is adopted, the starting materials are quickly heated, the fermentation time is shortened, a deodorizing device is not needed, organic wastes are thoroughly degraded, and the emission of malodorous gases such as sulfide and nitrogen oxide is greatly reduced;

2. the composite strain is obtained by screening, so that the application range is wide, the efficiency is high, and antagonism cannot be generated;

3. the organic fertilizer prepared by fermentation has high quality, the total organic matter content in the organic fertilizer is more than 69.1%, the organic matter content is more than 64.9%, and the content of the easily-oxidized organic matter is more than 26.3%, so that the organic fertilizer meets the national organic fertilizer standard;

4. the organic fertilizer prepared by the application can improve soil, promote plant growth and improve the yield of crops.

Detailed Description

The present application will be described in further detail with reference to examples.

Raw materials

The main sources of the raw materials in the present application are shown in table 1, and the raw materials not listed in table 1 are all commercially available without particular requirements.

TABLE 1 major sources of raw materials

Preparation example

In the present application, the addition of composite strains including mesophilic bacteria, thermophilic bacteria and growth-promoting bacteria can accelerate the temperature rise and shorten the fermentation time in the fermentation process of the starting material, and the weight ratio of the mesophilic bacteria, thermophilic bacteria and growth-promoting bacteria is specifically shown in table 2.

TABLE 2 preparation of Complex strains

Categories	Mesophilic bacteria: thermophilic bacteria: growth-promoting bacterium
		Preparation example 1	1:2.1:1
Preparation example 2	1:2.4:1
		Preparation example 3	1:2.8:1
Preparation example 4	1:3.0:1

Examples

A fermentation method of organic fertilizer is prepared through the following steps,

s1: the starting material comprises 400 parts of livestock and poultry manure and 200 parts of regulating material, wherein the water content of the starting material is 45-55%, the weight ratio of carbon to nitrogen is (25-35) to 1, and the weight ratio of active carbon to nitrogen is (20-25): 1, wherein the active organic carbon accounts for 20-30% of the total carbon, and the quick-acting nitrogen accounts for 30-35% of the total nitrogen;

s2: adding composite strain 0.1-1.2% of the initial material;

s3: adding a fermentation function synergist which comprises, by weight, 50-90 parts of kitchen fermented biological humic acid, 5-10 parts of quick lime, 0.01-0.1 part of polyaspartic acid, 6-12 parts of vermiculite powder, 2-5 parts of zeolite powder and 10-15 parts of a nitrification inhibitor;

s4: and (3) back mixing, wherein the fermentation process comprises the initial fermentation stage, the middle fermentation stage and the later fermentation stage, and the materials in the later fermentation stage are back mixed for 3-5 days, and the back mixing amount is 10-30% of the initial materials.

Example 1

S1: the starting material comprises 300kg of pig manure and 200kg of corn straw, wherein the water content of the starting material is 50%, the ratio of carbon to nitrogen is 25:1, active organic carbon accounts for 25% of total carbon, and quick-acting nitrogen accounts for 30% of total nitrogen

S2: adding composite strains, wherein the adding amount of the composite strains is 0.5kg (which is 0.1 percent of the starting material), the composite strains adopt a preparation example 1, the weight ratio of micrococcus luteus, clostridium cellulolyticum and bacillus firmus in mesophilic bacteria is 2:2:3, and the weight ratio of azospirillum, alcaligenes and mesorhizobium in growth-promoting bacteria is 1:1: 1.5;

s3: adding a fermentation function synergist which comprises 50kg of kitchen fermented biological humic acid, 5kg of quick lime, 0.01kg of polyaspartic acid, 6kg of vermiculite powder, 2kg of zeolite powder and 10kg of nitrification inhibitor, wherein the nitrification inhibitor is 2-chloro-6- (trichlorotoluene) pyridine;

s4: and (3) back mixing, wherein the fermentation process comprises an initial fermentation stage, a middle fermentation stage and a later fermentation stage, and the materials in the later fermentation stage are back mixed for 4 days, and the back mixing amount is 50kg (which is 10 percent of the initial materials).

The following are the internal parameters referred to in examples 1 to 7, and are specifically shown in table 3.

TABLE 3 parameters (unit: kg) in examples 1 to 7

Categories	Adding amount of composite strain	Preparation example	Amount of back-mixing
				Practice ofExample 1	0.5	1	50
Example 2	3	1	50
				Example 3	6	1	50
Example 4	3	2	50
				Example 5	3	3	50
Example 6	3	2	100
				Example 7	3	2	150

Example 8

Example 8 differs from example 6 in that example 8 also includes 0.05kg of alpha-naphthylacetic acid.

Example 9

Example 9 differs from example 6 in that 2.46g of veratryl alcohol inducer, 6.56g of tween 80 were added to example 9.

Comparative example

Comparative example 1

Comparative example 1 is different from example 6 in that step S2 is not included in comparative example 1.

Comparative example 2

Comparative example 2 is different from example 6 in that step S3 is not included in comparative example 3.

Comparative example 3

Comparative example 3 is different from example 6 in that step S4 is not included in comparative example 3.

The differences between comparative examples 4 to 6 and example 7 are shown in table 4.

TABLE 4 parameters (unit: kg) in comparative examples 4 to 6

Categories	Adding amount of composite strain	Preparation example	Amount of back-mixing
				Comparative example 4	0.45	2	100
Comparative example 5	3	4	100
				Comparative example 6	3	2	180

Performance test

Test one: detection of fermentation stage

Test field: surplus shed village in suitable city of Hubei province

1. Fermentation time detection

The starting materials are equally divided into 15 parts for stacking, the 15 parts are respectively stacked in rectangular fermentation tanks with the width and the height of 2m and 1m, and the inoculation of the compound bacteria and the fermentation function synergist are respectively carried out according to the requirements of examples 1-9 and comparative examples 1-6. Recording the time from the initial stage of fermentation to the middle stage of fermentation from 45 ℃ to 65 ℃, namely the temperature rise time; the time taken from the start of the batch to the end of the middle fermentation period when the temperature dropped to 40 ℃ was recorded as the fermentation time.

2. Released gas detection

In the test process, 3 points in the middle of fermentation are collected as detection (beginning of the middle of fermentation, the third day of the middle of fermentation and ending of the middle of fermentation), and the average values are obtained after the detection is respectively recorded. The concentrations of hydrogen sulfide and ammonia in the gas sample in the test were determined by gas chromatography Agilent GC 7890 under the following conditions: the system uses a CA-5 gas sample injection instrument to inject samples, sends out instructions to control the switch of a solenoid valve through a microcomputer program, synchronously injects the samples through 2 sample injection ports to analyze the concentration of hydrogen sulfide and ammonia in the gas at one time, and the effective air input of each sample injection port is 5 ml.

According to GB14554-1993 discharge standard of malodorous pollutants, the first-class discharge standard of the factory boundary of ammonia gas is 1.0mg/m³(ii) a The first-level emission standard of hydrogen sulfide in the factory is 0.03mg/m³。

TABLE 5 fermentation stage test results

And (2) test II: detection of fermented organic fertilizer

Collecting 15 parts of fermented organic fertilizer for detection, collecting samples on the upper layer, the middle layer and the lower layer of each part of organic fertilizer respectively, wherein the total amount of the samples is 120g, the samples are averagely divided into two parts, one part of the sample is collected in a plastic bag and sealed, and the two parts are refrigerated and stored at the temperature of 4 ℃; another sample is tested according to the standard of NY/T2876-2015 fertilizer and soil conditioner organic matter grading measurement, and the specific test results are shown in Table 6.

Table 6 organic fertilizer test results

And (3) test III: organic fertilizer is applied to the growth process of planting eggplants and hot peppers

The organic fertilizers prepared in examples 1-9 and comparative examples 1-6 in the application are applied to crops, and the yield detection is carried out by taking eggplants and peppers as examples. The eggplant and the pepper are planted according to the conventional method in the field, and in the cultivation process, the cultivation is the same except that the adopted organic fertilizer is different. The yield of the eggplant and the pepper in the mature period is detected, the yield is calculated, and the specific result is shown in table 7:

TABLE 7 yield of eggplant and pepper (greenhouse planting)

Table 7 open white controls are based on yield increase calculations, "-" indicates no yield increase.

It can be seen by combining examples 1-9 and comparative examples 1-6 and combining table 5 that the technical solutions of examples 1-8 in the present application can achieve rapid temperature rise of organic fertilizer, can reach the middle stage of fermentation within 24.1h, has short fermentation time, and generates little malodorous gas hydrogen sulfide and ammonia gas during fermentation, thus completely meeting the first-level emission standard of national factory boundaries.

By combining examples 1-9 and comparative examples 1-6 and combining table 6, it can be seen that the organic fertilizer obtained by the technical scheme of examples 1-9 in the application has high organic matter content, wherein the total organic matter content is above 69.1%, the organic matter content is above 64.9%, and the easily-oxidized organic matter content is above 26.3%.

Combining example 6 and comparative examples 1-3 with tables 5 and 6, it can be seen that the absence of steps S2, S3 or S4 in the process of preparing organic fertilizer results in slow temperature rise and more malodorous gas release during the fermentation process; the organic matter content in the finally obtained organic fertilizer is low.

By combining examples 1-9 and comparative examples 1-6 and combining table 7, it can be seen that the yield can be improved by applying the organic fertilizer obtained by the technical scheme of examples 1-9 in the application to eggplants and peppers, and the yield is increased by more than 23%.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.