阅读说明：本技术 一种防治植物细菌性病害的化合物及应用 (Compound for preventing and treating plant bacterial diseases and application thereof ) 是由 叶火春 张静 朱发娣 闫超 辜柳霜 冯岗 于 2021-09-30 设计创作，主要内容包括：本发明公开了防治植物细菌性病害的化合物及应用,所述化合物为萝卜硫素。在离体条件下,萝卜硫素具有抑制细菌活性,该化合物在供试浓度为1000mg/L时,对柑橘溃疡病菌、芒果细菌性角斑病菌、辣椒青枯病菌、水稻细菌性条斑病菌、草莓细菌性角斑病菌、马铃薯软腐病菌、木薯细菌性萎蔫病菌具有很好的抑菌作用,抑制率均在90％以上；同时,活体试验结果表明,萝卜硫素对辣椒青枯病、水稻细菌性条斑病和芒果细菌性角斑病也具有良好的防治效果,可用于农业细菌病害的防治。本发明将萝卜硫素作为活性物质用于防治植物细菌性病害的杀菌剂或抑菌剂,为农业病害防治中提供了一种天然源杀菌活性物质,其高效、低毒、安全等特点符合当前新农药创制的要求。(The invention discloses a compound for preventing and treating plant bacterial diseases and application thereof, wherein the compound is sulforaphane. Under the in vitro condition, sulforaphane has the function of inhibiting bacterial activity, and the compound has good bacteriostatic action on citrus canker, mango bacterial angular leaf spot, pepper ralstonia solanacearum, rice bacterial streak germ, strawberry bacterial angular leaf spot, potato soft rot germ and cassava bacterial wilt germ when the test concentration is 1000mg/L, and the inhibition rate is over 90 percent; meanwhile, the in vivo test result shows that the sulforaphane also has good control effect on pepper bacterial wilt, rice bacterial streak and mango bacterial angular leaf spot, and can be used for controlling agricultural bacterial diseases. The sulforaphane is used as an active substance for a bactericide or a bacteriostatic agent for preventing and treating plant bacterial diseases, a natural source bactericidal active substance is provided for preventing and treating agricultural diseases, and the characteristics of high efficiency, low toxicity, safety and the like meet the requirements of creating new pesticides at present.)

1. The compound for preventing and controlling the bacterial diseases of plants is sulforaphane, and the structural formula of the compound is as follows:

2. the sulforaphane is used for preventing and treating plant bacterial diseases, and is characterized in that pathogenic bacteria of the diseases are mango bacterial angular leaf spot bacteria campholris pv. mangiferae, melon bacterial fruit spot bacteria Acidovorax citrulli, Capsicum solanacearum Ralstonia solanacearum, rice bacterial stripe disease Xanthomonas oryzae pv. oryzicola, citrus canker bacterium Xanthomonas pv. citri, Actinidia canker pathogen sympathode pv. actinonian, strawberry bacterial angular leaf spot bacteria Xanthomonas fragaria, Chinese cabbage soft rot bacteria Pectobacterium subsp. carotovorum, potato soft rot bacteria Pectobacterium subsp. brasiliensis and/or bacterial fruit blight.

3. The application of sulforaphane in preventing and treating plant bacterial diseases is characterized by being used for preventing and treating pepper bacterial wilt, rice bacterial streak and/or mango bacterial angular leaf spot.

4. The bacteriostatic agent for preventing and treating plant bacterial diseases is characterized in that the active ingredient of the bacteriostatic agent is sulforaphane, the sulforaphane content is 0.05-60% by weight, and auxiliary agents are added.

5. The bactericide for preventing and treating plant bacterial diseases is characterized in that the active ingredient of the bactericide is sulforaphane, the content of the sulforaphane is 0.05 to 60 percent by weight, and an auxiliary agent is added.

6. The bacteriostatic agent for controlling plant bacterial diseases according to claim 4 or the bactericide for controlling plant bacterial diseases according to claim 5, wherein the dosage form of the bacteriostatic agent or the bactericide is one of emulsifiable concentrate, suspending agent, aqueous emulsion, microemulsion, wettable powder or water dispersible granules.

7. A bacteriostatic agent for controlling plant bacterial diseases according to claim 4 or a bacteriocide for controlling plant bacterial diseases according to claim 5, which is applied to control plant bacterial diseases, and pathogenic bacteria of the diseases are mango bacterial angular leaf spot bacterium campholaris pv. mangiferandica, melon bacterial fruit spot bacterium Acidovorax citrulli, Ralstonia capsici solanaceum, rice bacterial stripe spot Xanthomonas oryzae pv. oryzicola, Citrus canker Xanthomonas. citri trip pv. cithi pv. citri, Actinidia kiwii ulcer bacterium Pseudomonas syringae pv. acta, strawberry bacterial angular leaf spot bacterium Xanthomonas fragaria, Pectinophora brassicae, Pectinopus brassicae, Pectinoporus brassica subsp.

8. An application of a sulforaphane-containing bacteriostatic agent in preventing and treating plant bacterial diseases.

9. An application of a disinfectant containing sulforaphane in preventing and treating plant bacterial diseases.

Technical Field

The invention belongs to the technical field of plant chemical protection, and particularly relates to a compound for preventing and treating plant bacterial diseases and application thereof.

Background

In the crop planting process, bacterial diseases are important diseases threatening the production of crops, such as rice bacterial blight, rice bacterial leaf streak, potato soft rot, Chinese cabbage soft rot, citrus canker, mango bacterial angular leaf spot and the like. Because of few disease resistant varieties, chemical prevention and control are still mainly adopted for preventing and controlling bacterial diseases at present, and prevention and control agents mainly depend on copper agents and agricultural antibiotic bactericides, such as copper hydroxide, copper oxychloride, oxine-copper, thiediazole-copper, zhongshengmycin, nanningmycin and the like. However, with the frequent and unreasonable use of these bactericides, the problem of environmental pollution caused by copper preparations is obvious, the level of resistance of agricultural antibiotics is increased, the risk of resistance shared by medical antibiotics exists, and streptomycin which is commonly used in the production is prohibited. In order to reduce agricultural production loss and environmental pollution and alleviate the problem of disease resistance, the development of novel control agents is urgently needed. The plant source natural products have the characteristics of various types, environmental friendliness, novel action mode and the like, and are important objects for researching and developing novel pesticides at present.

Sulforaphane (Sulforaphane), also known as Sulforaphane, has the chemical name of 1-isothiocyanato-4-methylsulfonylbutane. Sulforaphane is widely found in cruciferae plants such as broccoli, red cabbage, water spinach, onion, etc., and is obtained by hydrolyzing glucosinolates in the plant body with myrosinase (myrosinase enzyme). In addition, the sulforaphane can be synthesized by taking 1-bromo-3-chloropropane, ammonium thiocyanate and anhydrous acetonitrile as raw materials, and a rich source is provided for the sulforaphane. At present, the research on the sulforaphane at home and abroad mainly focuses on the preparation method, chemical synthesis and medical activity of the sulforaphane, and the application of the sulforaphane is focused on the medicine aspect at present, but the report of the sulforaphane applied to the inhibition of pathogenic bacteria of agricultural plants is not seen.

Disclosure of Invention

In view of one or more of the above-mentioned deficiencies or needs for improvement in the prior art, the present invention provides a compound for controlling plant bacterial diseases, which is sulforaphane, having a significant inhibitory effect on various plant pathogenic bacteria, and its use.

To achieve the above objects, according to one aspect of the present invention, there is provided a compound for controlling bacterial diseases of plants, wherein the compound is sulforaphane, and the structural formula of the compound is as follows:

according to a second aspect of the present invention there is provided the use of sulforaphane in the control of bacterial diseases in plants, the pathogens of which are mango bacterial angular leaf spot pathogen Xanthomonas campestris pv. mangiferae, melon bacterial fruit spot pathogen Acidovorax citrulli, Ralstonia capsici, solanum solanacearum, rice bacterial stripe spot Xanthomonas oryzae pv. oryzicola, Citrus canker Xanthomonas citri pv. citri, Actinidia canker Pseudomonas syringae pv. actinoides, Micromonas campestris Xanthomonas campestris, Pectinophyta brasiliensis and/or Microbacterium solani.

In addition, the sulforaphane can also be used for preventing and treating pepper bacterial wilt, rice bacterial leaf streak and/or mango bacterial angular leaf spot.

According to a third aspect of the invention, the invention provides a bacteriostatic agent and a bactericide for controlling plant bacterial diseases, wherein the sulforaphane content is 0.05-60% by weight, and auxiliary agents are added. Furthermore, the inhibitor or the bactericide is in the form of one of missible oil, suspending agent, aqueous emulsion, microemulsion, wettable powder or water dispersible granule.

Further, the bacteriostatic agent and the bactericide can be applied to control plant bacterial diseases, and pathogenic bacteria of the diseases are mango bacterial angular leaf spot bacterium camphollison pv. mangiferae, melon bacterial fruit spot bacterium Acidovorax citrulli, Capsicum solanacearum Ralstonia solanacearum, rice bacterial leaf streak disease Xanthomonas oryzae pv. oryzicola, citrus canker bacterium xanthium circ pv. citri, Actinidia corynescensis syzygium pv. actinium, strawberry bacterial angular leaf spot bacterium Xanthomonas fragaria, Pectobacterium brassicae, Pectinatum carotovorum subsp.

According to a fourth aspect of the present invention, there is provided the use of a sulforaphane-containing bacteriostatic or bacteriocidal agent for controlling plant bacterial diseases.

Generally, compared with the prior art, the above technical solution conceived by the present invention has the following beneficial effects:

the sulforaphane is a plant secondary metabolite, has low residue and high environmental safety in the process of preventing and treating agricultural bacterial diseases; the sulforaphane has broad-spectrum bacterial activity inhibition, and the use of chemical pesticides or antibiotics can be reduced by using the sulforaphane to prevent and treat agricultural bacterial diseases, so that the problem of drug resistance is relieved.

In-vitro tests show that sulforaphane has a remarkable inhibitory effect on various plant pathogenic bacteria at a concentration of 1000mg/L, has a good inhibitory effect on mango bacterial angular leaf spot, citrus canker, rice bacterial streak, strawberry bacterial angular leaf spot, pepper bacterial wilt and cassava bacterial wilt, has an inhibitory rate of over 90 percent, has a certain inhibitory effect on cabbage soft rot and melon bacterial fruit leaf spot, and has an inhibitory rate of 55.34 percent and 50.61 percent. The results of in vivo tests show that the compound has a good inhibition effect on the pathogenicity of the pseudomonas solanacearum and has a good control effect on the bacterial leaf streak of rice and the bacterial angular leaf streak of mango.

In a word, the compound provided by the invention has a remarkable inhibiting effect on plant pathogenic bacteria, can be used for preventing and treating various important agricultural diseases such as pepper bacterial wilt, rice bacterial streak, mango bacterial angular leaf spot and the like, and provides a natural source bactericidal active substance for preventing and treating the agricultural diseases; the compound is used as a bactericide or a bacteriostatic agent, and has the characteristics of high efficiency, low toxicity, safety and the like, which meet the requirements of creating new pesticides at present.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

The invention provides an application of sulforaphane in preventing and treating plant bacterial diseases. The specific diseases are plant bacterial diseases: mango bacterial angular leaf spot bacterium, Xanthomonas campestris pv. mangiferae, melon bacterial fruit leaf spot bacterium Acidovorax citrulli, Ralstonia solanacearum, rice bacterial streak bacterium Xanthomonas oryzae pv. oryzicola, Citrus canker bacterium iv. citri, Actinidia chinensis canker bacterium syphon pv. actinoniae, strawberry bacterial angular leaf spot bacterium Xanthomonas fragaria, Pectobacterium carotovorum subsp. carotovorum, Pectobacterium carotovorum subsp. brasiliensis, and Manihothiobacter xylinum. In addition, the in-vivo test result shows that the sulforaphane has a good inhibition effect on the bacterial wilt of the capsicum and also has a good control effect on the bacterial leaf streak of the rice and the bacterial angular leaf spot of the mango.

The invention also provides a bactericide or bacteriostatic agent for preventing and treating plant bacterial diseases, which comprises the active ingredient sulforaphane, the content of the active ingredient sulforaphane is 0.05-60 percent (mass weight), and optional auxiliary agents. Aiming at the preparation, according to actual needs, the preparation can be prepared into one of missible oil, a suspending agent, an aqueous emulsion, a microemulsion, wettable powder or water dispersible granules by adopting a conventional method in the field, and the preferable preparation formulation is the missible oil, the aqueous emulsion and the microemulsion.

The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.

The materials, reagents and the like used in the examples of the present invention can be obtained commercially unless otherwise specified.

In the preparation process of the preparation, the adopted auxiliaries are all conventional auxiliaries in the field, and a person skilled in the art can select the corresponding auxiliaries according to the dosage form of the prepared inhibitor or bactericide, and the percentages are weight percentages.

Example 1

The embodiment is to prepare a 5% sulforaphane microemulsion, and the 5% sulforaphane microemulsion is prepared by weighing 5 parts of sulforaphane, 20 parts of styrene phenol polyvinyl ether phosphate, 500# agricultural emulsion, 10 parts of dimethyl sulfoxide, 10 parts of xylene and 4 parts of ethylene glycol by 100 parts by mass, stirring to fully dissolve the sulforaphane, supplementing the balance by deionized water, and continuously stirring to a homogeneous phase.

Example 2

The embodiment is preparation of a 2% sulforaphane aqueous emulsion, and the 2% sulforaphane aqueous emulsion is prepared by weighing 2 parts of sulforaphane, 6 parts of triphenylethylphenol polyoxyethylene ether, 4 parts of nonylphenol polyoxyethylene ether, 4 parts of dimethyl sulfoxide, 6 parts of xylene, 5 parts of castor oil, 0.5 part of n-butyl alcohol, 5 parts of isopropanol and 0.2 part of xanthan gum by 100 parts by mass, supplementing the balance with deionized water, mixing the raw materials, and shearing, stirring and dispersing at a high speed.

Example 3

The example is the preparation of 20% sulforaphane emulsifiable concentrate, the preparation method is a conventional emulsifiable concentrate preparation method, and the used auxiliary agent is a conventional auxiliary agent of emulsifiable concentrate preparations in the field, and the details are not repeated.

Test example 1 measurement of inhibitory Activity of sulforaphane bacteriostatic agent against plant pathogenic bacteria

(1) Test strains

Mango bacterial angular leaf spot pathogen, melon bacterial fruit leaf spot pathogen Acidovorax citrulli, Ralstonia solanacearum, rice bacterial leaf spot pathogen Xanthomonas oryzae pv oryzae, Citrus canker bacterial pv. citri, Actinidia kiwii canker pathogen, Pseudomonas syringae pv. actandiae, strawberry bacterial angular leaf spot pathogen Xanthomonas fragaria, Pectinopus brassicae subsp. The strains are provided by the group of the environment of the Chinese tropical agricultural academy of sciences and the chemical biology and application subjects of pesticides of the plant protection research institute.

(2) Test method

(2.1) Ex vivo Activity assay

In order to evaluate the inhibition effect of sulforaphane on plant pathogenic bacteria, the minimum inhibitory concentration of the compound on the plant pathogenic bacteria is measured by a turbidity method (96-hole enzyme label plate) to evaluate the inhibitory activity of the compound on the plant pathogenic bacteria. The method refers to the inhibiting effect and mechanism of protocatechualdehyde on Enterobacter sakazakii [ J]In modern food technology, 2017,33(07):105-⁹cfu/mL) was added to a 96-well plate at 10. mu.L per well. Adding culture solution containing no drug into control group, and adding 200 μ L per well; the test group was prepared by adding 200. mu.L of each well of each drug-containing culture medium at each concentration. Meanwhile, the control group and the test group are both provided with a background group without inoculated bacterial liquid. Blowing and uniformly mixing the reagent solution and the bacterial suspension, placing the sample treatment plate in an incubator for vibration (500r/min) culture, setting the culture temperature to be 28-30 ℃, culturing for 18-24 h, and measuring the OD of the culture bacterial solution by using an enzyme-linked immunosorbent assay₆₀₀The value is obtained. The inhibitory effect of the compounds on pathogenic bacteria was calculated by the following formula.

Bacteriostatic rate (%) { (control OD)₆₀₀Control background group OD₆₀₀) - (test group OD)₆₀₀-Compound background group OD₆₀₀) }/(control group OD₆₀₀Control background group OD₆₀₀) X 100% (formula 1)

And (4) analyzing results:

the results of the bacteriostatic activity of the sulforaphane to the plant pathogenic bacteria of test 10 are shown in table 1, and the results show that the sulforaphane to be tested has good bacteriostatic action on rice bacterial streak germs, citrus canker germs, cassava bacterial wilt germs, pepper ralstonia solanacearum, strawberry bacterial angular leaf spot germs, mango bacterial angular leaf spot germs and potato soft rot germs when the concentration of the sulforaphane to be tested is 1000mg/L, the inhibition rates are all over 90 percent, and the sulforaphane has certain inhibitory activity on cabbage soft rot germs and melon bacterial fruit rot germs, and the inhibition rates are 55.34 percent and 50.61 percent.

TABLE 1 bacteriostatic Activity of sulforaphane against 10 plant pathogenic bacteria

Test strains	Inhibition ratio (%)	Test strains	Inhibition ratio (%)
				Bacterial angular leaf spot of mango	93.76	Strawberry bacterial angular leaf spot	94.24
Bacterial fruit blotch of melon	50.61	Soft rot of Chinese cabbage	55.34
				Bacterial wilt of hot pepper	98.54	Soft rot of potato	93.11
Bacterial leaf streak germ of rice	94.60	Bacterial wilt of cassava	95.86
				Kiwifruit canker pathogen	81.31	Pathogenic bacteria of citrus canker	96.62

Based on the above screening results, the Minimum Inhibitory Concentration (MIC) of sulforaphane against 8 plant pathogenic bacteria such as mango bacterial angular leaf spot pathogen was determined (as shown in Table 2). The result shows that the sulforaphane has good in-vitro activity on the ralstonia solanacearum of rice, and the MIC of the sulforaphane is 460 mg/L; the in vitro inhibition effect on melon bacterial fruit blotch is weak, and the MIC is 1667 mg/L.

TABLE 2 minimal inhibitory concentration and sub-inhibitory concentration of sulforaphane against 8 kinds of germs such as rice bacterial streak germ

Test germs	MIC(mg/L)
		Bacterial angular leaf spot of mango	1000
Bacterial wilt of hot pepper	460
		Pathogenic bacteria of citrus canker	1400
Bacterial leaf streak germ of rice	800
		Strawberry bacterial angular leaf spot	1200
Soft rot of potato	750
		Bacterial wilt of cassava	1200
Bacterial fruit blotch of melon	1667

Test example 2 Effect of sulforaphane on prevention of bacterial leaf streak of rice

The injury inoculation method is adopted to test the control effect of sulforaphane on the bacterial leaf streak pot culture of rice. Sowing rice seeds (Nipponbare) in seedling pots, transferring the seedlings to flowerpots when the seedlings grow to be 15cm high, transplanting 2 rice seedlings in each flowerpot, selecting rice seedling pots with relatively consistent growth vigor after the seedlings are transplanted to survive, and repeatedly treating 10 rice seedlings in each flowerpot. After the transplanted seedling grows to the tillering stage, a wound is formed at the 2cm cut-off position of the tip end of the rice leaf by using sterilized scissors. Soaking 5 treatment solutions of sulforaphane (1000, 500, 250mg/L), 2% kasugamycin aqueous solution and sterile water in rice leaf wound, air drying, repeatedly soaking for 2 times, inoculating bacterial suspension of alternaria alternate (OD)₆₀₀About 0.8 deg.c), placed at 28-30 deg.c, relative humidity 90 + -5 deg.c, light/dark cycle 14/10h, each treatment was repeated 4 times. After 14 days of culture, the disease condition was observed and recordedReference is made to the literature (identification of Enterobacter cloacae MY01 and study on prevention and treatment of bacterial leaf streak in rice [ J]Plant pathology newspaper, 2020, 50 (4): 471-478), and calculating the control effect according to the formula (2) and the formula (3).

Disease grade:

level 0: no obvious scab is generated at the cut;

level 1: only small spots of translucent water ulceration disease spots occupy less than 1% of the leaf area;

and 3, level: the disease spots are sporadically short and narrow and occupy 1 to 5 percent of the leaf area;

and 5, stage: the disease spots are more and account for 6 to 25 percent of the area of the leaves;

and 7, stage: the disease spots are dense and occupy 26 to 50 percent of the area of the leaves;

and 9, stage: the lesion area is more than 50%, and the leaves turn orange brown, curl and die.

The calculation formula of the drug effect is as follows:

and (4) analyzing results:

the rice pot experiment result shows that the sulforaphane has good protection and prevention effects on the bacterial leaf streak of rice. When the treatment concentration of the 20% sulforaphane emulsifiable solution is 1000mg/L, the control effect on the bacterial leaf streak of rice reaches 76.67%, and the control effect of the treatment concentration of 500mg/L is better than that of the treatment with 2% kasugamycin aqueous solution. The sulforaphane has the protective effect of over 40 percent at 250 mg/L.

TABLE 3 sulforaphane control of bacterial leaf streak in rice potting test

Test example 3 potted plant control effect test of sulforaphane on pepper bacterial wilt

When pepper seedlings grow to 3-4 pieces, treating 10mL of sulforaphane with different concentrations (2000mg/L, 1000mg/L and 500mg/L), 2% kasugamycin aqueous solution 1000mg/L and clear water 5 respectively, irrigating roots for 3 times at intervals of 2d, and irrigating roots for 1 time after 3 times, and irrigating 2mL of ralstonia solanacearum (OD) along the position 3cm away from the ground along pepper seedling stems₆₀₀0.8-1.0), treating 20 plants each time, continuously culturing under the conditions of 29-32 ℃ and 14h/10 h photoperiod, observing and recording the number of diseased plants in 14-28d, recording and counting according to the record statistics of 'bactericide control on tomato bacterial wilt' (NY/T1464.32-2010) in part 32 of 'efficacy guidelines', and calculating the control effect according to formula 4 and formula 5.

Incidence (%) ═ number of diseased plants/number of investigated plants × 100 (formula 4)

Control efficacy = (incidence after drug administration in control group-incidence after drug administration in treatment group)/incidence after drug administration in control group x 100 (formula 5)

And (4) analyzing results:

the pepper pot experiment result shows that the sulforaphane has stronger protection and control effects on pepper bacterial wilt. After inoculation and culture for 21 days, the protective effect of the sulforaphane with the treatment concentration of 2000mg/L on ralstonia solanacearum is 84.59%, and the protective effect of the sulforaphane with the treatment concentration of 1000mg/L is higher than the protective effect of a control medicament, namely a 2% kasugamycin aqueous solution; after the culture is carried out for 28 days, the treatment concentration of the sulforaphane is 2000mg/L, the control effect on the pepper bacterial wilt is still over 70 percent, and the control effect is obviously better than that of a 2 percent kasugamycin aqueous solution.

TABLE 4 Pot culture test of sulforaphane for bacterial wilt of hot pepper

Test example 4 potting test of sulforaphane against the mango bacterium angular leaf spot

After 5d of potted mango (variety: Tainong) young shoots, sulforaphane (1000, 500 and 250mg/L), 4% kasugamycin water aqua 500mg/L and 5 clear water are uniformly treated and sprayed on mango leaves, and after the mango leaves are naturally dried, mango bacterial angular leaf spot bacterium suspension (OD) containing 0.1% tween-20 is naturally dried₆₀₀About 1.0), a nozzleInoculating onto mango leaf, treating 3 young shoots each, repeating for 3 times, culturing under humid illumination for 10d and 20d, observing disease condition, investigating disease index (formula 6), and calculating preventing and treating effect (formula 7). Bacterial angular leaf spot disease grading Standard [ refer to pesticide II for Bactericidal control of Citrus canker ]]：

Level 0: no disease spots;

level 1: each leaf has 1-5 scabs;

and 3, level: each leaf has 6-10 scabs;

and 5, stage: each leaf has 11-15 scabs;

and 7, stage: each leaf has 16-20 scabs;

and 9, stage: each leaf has more than 21 scabs;

the calculation formula of the drug effect is as follows:

and (4) analyzing results:

the result shows that the sulforaphane has strong prevention and treatment effect on mango bacterial angular leaf spot, and the prevention and treatment effect on mango bacterial angular leaf spot is 71.38% when the test concentration is 1000 mg/L; when the concentration of the test reagent is 500mg/L, the control effect on the disease is still 62.48 percent, which is equivalent to the control effect of a 2 percent kasugamycin aqueous solution serving as a reference reagent, and when the concentration of the test reagent is reduced to 250mg/L, the control effect on the bacterial angular leaf spot of mango is not good.

TABLE 5 test of the efficacy of sulforaphane on bacterial angular leaf spot of mango

It will be understood by those skilled in the art that the foregoing is only a preferred embodiment of the present invention, and is not intended to limit the invention, and that any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.