阅读说明：本技术 一种杀虫组合物 (Insecticidal composition ) 是由 罗昌炎 詹姆斯.T.布里斯托 于 2020-07-02 设计创作，主要内容包括：本发明涉及一种杀虫组合物,其含有活性成分化合物I和化合物II,化合物II选自虱螨脲。本发明还涉及所述杀虫组合物用于预防或控制菜蛾科、夜蛾科、灯蛾科、飞虱科、粉虱科、叶螨科、瘿螨科、蓟马科害虫的用途。化合物I。(The invention relates to an insecticidal composition, which contains active ingredients of a compound I and a compound II, wherein the compound II is selected from lufenuron. The invention also relates to application of the insecticidal composition in preventing or controlling pests of plutella, noctuidae, lampridae, plant hopper, whitefly, tetranychidae, gall mite and thrips.)

1. An insecticidal composition, which contains active ingredients of a compound I and a compound II,

compound I

The compound II is selected from lufenuron.

2. The insecticidal composition of claim 1, wherein the weight ratio of compound I to compound II is from 100:1 to 1:100, more preferably from 50:1 to 1:50, more preferably from 25:1 to 1:25, more preferably from 10:1 to 1: 10.

3. An insecticidal composition according to claim 1, further comprising a surfactant and/or a filler.

4. The insecticidal composition of claim 1 wherein the weight of compound I and compound II together comprise from 1% to 90%, preferably from 5% to 80%, more preferably from 5% to 70%, still more preferably from 5% to 60%, more preferably from 10% to 60% by weight of the insecticidal composition.

5. The insecticidal composition of claim 1, wherein the formulation of the insecticidal composition is emulsifiable concentrate, aqueous suspension, oil suspension, dry seed treatment powder, seed treatment suspension, water dispersible granule, wettable powder, suspoemulsion, aqueous emulsion, microcapsule suspension, dry suspension, ultra-low volume liquid, microgranule, and microemulsion.

6. Use of the pesticidal composition according to claim 1 for preventing or controlling lepidopteran, hemipteran, dipteran, thysanoptera, acarina pests and their larvae and eggs.

7. Use of the pesticidal composition of claim 1 for preventing or controlling diamondback moth, noctuidae, lampwick, planthopper, whitefly, tetranychidae, gall mite, thrips and larvae and eggs thereof.

8. Use of the insecticidal composition according to claim 1 for preventing or controlling tetranychus cinnabarinus, ticks, bemisia tabaci, whitefly, diamond back moth, prodenia litura, fall webworm, meadow moth, beet webworm, laodelphax striatellus, sogatella furcifera, thrips and larvae and eggs thereof.

9. A method for preventing or controlling pests and their larvae and eggs, characterized in that the pesticidal composition according to claim 1 is applied to the target useful plants, the target pests or their environment, propagation material of the target useful plants.

10. A method for preventing or controlling pests and their larvae and eggs, characterized in that the pesticidal composition of claim 1 is applied to seeds, target useful plants or soil where plants grow or soil suitable for plant growth.

11. A method for preventing or controlling pests and their larvae and eggs, characterized in that the pesticidal composition according to claim 1 is applied to the soil before, after or before germination of the seeds and/or directly to the soil in contact with the roots of the plants or to the soil suitable for the growth of the plants.

12. A method for protecting seeds, characterized in that the seeds are contacted with a pesticidally effective amount of the pesticidal composition according to claim 1 before sowing and/or after pregermination.

Technical Field

The present invention relates to an insecticidal composition which is particularly effective in controlling agricultural and forestry pests.

Background

The most typical problem in agricultural and horticultural pest control is the need to reduce the dosage rate of the active ingredient to reduce or avoid adverse environmental or toxicological effects, while still providing effective pest control.

Another difficulty in pest control is that repeated and exclusive use of a single pesticidal active ingredient leads in many cases to rapid selection of pests that either naturally or acclimatize to resistance to the active ingredient. Due to the multi-generation and adaptability of pests, the resistance of pests is formed in a rather short time. There is therefore also a need for pest control agents which act on both pests and their larvae and eggs.

Solving at least one problem such as improving biological properties, providing synergistic properties, reducing dosage rates, expanding the spectrum of activity, combining knock-down activity with long-lasting action, management of resistance to drugs to delay resistance is a long felt need for controlling agricultural or forestry pests.

Compound I is known from WO2006013896 and belongs to the class of quinoline derivatives.

Compound I

Disclosure of Invention

The invention aims to overcome the defects and provides an insecticidal composition, which contains active ingredients of a compound I and a compound II, wherein the combination of the compound I and the compound II can effectively prevent or control animal pests and larvae and eggs thereof; we have found that the combined or separate application of compound I and compound II or the sequential application of compound I and compound II gives a higher control effect on pests than is possible with the application of the individual compounds. The combination of compound I and compound II is effective, reduces dosage rate, and reduces environmental load, and compound II is selected from lufenuron.

The invention relates to an insecticidal composition which is realized by adopting the following technical scheme:

an insecticidal composition, which contains active ingredients of a compound I and a compound II,

compound I

The compound II is selected from lufenuron.

The weight ratio of the compound I and the compound II is usually in the range of 100:1 to 1:100, more preferably 50:1 to 1:50, more preferably 25:1 to 1:25, more preferably 10:1 to 1:10, and a synergistic effect is obtained.

The compound I and the compound II have completely different action mechanisms and action modes, and have the effects of contact poisoning, stomach poisoning, ovicidal action and oviposition rate and hatching rate reduction after combination, so that pests are killed more easily, and the generation of resistance is delayed.

The invention provides an insecticidal composition, which contains active ingredients of a compound I and a compound II, wherein the weight of the compound I and the compound II accounts for 1% -90%, preferably 5% -80%, more preferably 5% -70%, still more preferably 5% -60%, and more preferably 10% -60% of the weight of the insecticidal composition.

A pesticidal composition, which contains as active ingredients compound I and compound II, the amounts by weight of compound I and compound II together also amounting to 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% by weight of the pesticidal composition, for example.

An insecticidal composition containing active ingredients of compound I and compound II, wherein the insecticidal composition further comprises a surfactant and/or a filler.

The insecticidal composition provided by the invention is in the dosage forms of missible oil, aqueous suspension, oil suspension, seed treatment dry powder, seed treatment suspension, water dispersible granules, wettable powder, suspoemulsion, aqueous emulsion, microcapsule suspension, dry suspension, ultra-low volume liquid, microgranule and microemulsion.

The insecticidal composition of the present invention may be applied undiluted or diluted with water.

The pesticidal compositions according to the invention surprisingly exhibit an extremely high activity in the treatment of plants, propagation material of plants, soil in which plants grow, in the prevention or control of pests and their larvae and eggs.

The insecticidal composition of the present invention has excellent control efficacy against pests and their larvae and eggs at low doses.

In another aspect, the invention also provides the use of a pesticidal composition for the prevention or control of pests and their larvae and eggs.

In particular, the invention provides the use of a pesticidal composition for the prevention or control of lepidopteran, hemipteran, dipteran, thysanoptera, acarina pests.

The invention provides an application of an insecticidal composition in preventing or controlling diamondback moth, noctuidae, lamplight moth, plant hopper, whitefly, tetranychidae, gall mite, thrips and larvae and eggs thereof.

More particularly, the present invention provides the use of an insecticidal composition for the prevention or control of tetranychus cinnabarinus, ticks, bemisia tabaci, whitefly, diamond back moth, prodenia litura, fall webworm, spodoptera frugiperda, beet armyworm, laodelphax striatellus, sogatella furcifera, thrips and their larvae and eggs.

Use of the pesticidal composition according to the invention for treating seeds to prevent the seeds from being attacked by pests.

The pesticidal composition according to the present invention may be applied to seeds, plants or fruits of plants or soil where plants grow or soil suitable for plant growth.

In another aspect, the invention provides a method for preventing or controlling pests and their larvae and eggs by applying the pesticidal composition of the invention to a target useful plant, a target pest or its environment, propagation material of a target useful plant. The plant propagation material is a seedling, rhizome, nursery seedling, cutting or seed.

A method for preventing or controlling pests and their larvae and eggs by applying the pesticidal composition of the present invention to seeds, target useful plants or soil in which plants grow or soil suitable for plant growth.

A method for preventing or controlling pests and their larvae and eggs, by applying the pesticidal composition according to the invention to the soil before, after or before germination of the seeds and/or directly to the soil in contact with the roots of the plants or to the soil suitable for the growth of the plants.

A method of protecting seeds comprising contacting the seeds before sowing and/or after pregermination with a pesticidally effective amount of the pesticidal composition of the invention.

The plants may include: stems and leaves of plants, flowers of plants, fruits of plants, and the like.

The seed is selected from soybean, wheat, barley, rye, sorghum, peanut, sunflower, sugarcane, tobacco, rice, rape, cole, cabbage, beet, tomato, cowpea, carrot, cotton and corn seed.

A method for preventing or controlling pests, comprising administering compound I, compound II separately, sequentially or simultaneously.

The pesticidal composition according to the invention can be applied in any desired manner, for example seed coating, soil drenching and/or direct furrow application and/or foliar spraying, and before, after or before germination.

The invention relates to an insecticidal composition containing active ingredients of a compound I and a compound II, which solves at least one problem such as improvement of biological characteristics, provision of synergistic characteristics, reduction of dosage rate, enlargement of activity spectrum, combination of knockdown activity and long-acting effect, and management of drug resistance to delay resistance.

Detailed Description

For the purpose of facilitating an understanding of the present invention, the present invention will now be described by way of examples. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

The invention discovers that: the combination of compound I and compound II is effective in preventing or controlling pests and their larvae and eggs. Surprisingly, the pest-controlling activity of the insecticidal compositions of the present invention is much higher than the sum of the properties of the individual active compounds. This unexpected effect is a true synergistic effect, not just a summation of activities.

The term "synergistic" as used herein means that the pesticidal effect of the active compound combinations or compositions of the present invention is greater than the sum of the effects of the individual active compounds, or that the effect is superadditive.

The invention provides an insecticidal composition, which contains active ingredients of a compound I and a compound II, wherein the compound II is selected from lufenuron.

The weight ratio of the compound I and the compound II is usually in the range of 100:1 to 1:100, more preferably 50:1 to 1:50, more preferably 25:1 to 1:25, more preferably 10:1 to 1:10, and a synergistic effect is obtained.

The weight ratio of compound I and compound II according to the invention may also be, for example: 100:1, 50:1, 45:1, 40:1, 35:1, 30:1, 25:1, 20:1, 19:1, 18:1, 17:1, 16:1, 15:1, 14:1, 13:1, 12:1, 11:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, 1: 100.

The preparation of compounds II and their action on pests is known. Can be found in The pesticide Manual, 16 th edition and other publications.

The pesticidal compositions according to the invention, which have good plant compatibility and favourable warm-blooded animal toxicity, are suitable for controlling pests encountered in agriculture, forestry, the protection of stored products and materials and in the hygiene sector, in particular Insecta (Insecta), Arachnida (Arachnida), Nematoda (Nematoda). They are active against normally sensitive and resistant species and against all or individual developmental stages, and also achieve a surprising "synergistic" effect in preventing or controlling pests.

The invention also provides a use of the insecticidal composition of the invention for preventing or controlling pests. The term "pest" is used herein to mean any organism that can cause loss of the normal state of a plant. Including Insecta (Insecta), Arachnida (Arachnida), and Nematoda (Nematoda).

The Insecta includes Lepidoptera, Coleoptera, Diptera, Hemiptera, Homoptera, Hymenoptera, Thysanoptera, Isopoda, Diplopoda, Symphyta, Thysanoptera, Rhamnoidea, Orthoptera, Dermaptera, Isoptera, and Anoplura.

Lepidopteran pests: the families of the borer moth (Pyralidae) such as chilo suppressalis (chilblains (walker), tryporyza incertulas (walker), cnaphalocrocis medinalis guene (cnaphalocrocis medinalis), cabbage borer (hellulaundalis), peach borer (Conogethespunctiviris), the families of the whiteflies (Pieridae) such as Pieris rapae (Pieriis rapae), Pieris citrifolia (papilioxutatus), white butterfly (Pierisrapaeecruscula), Orthosiphon oryza sativa (pararaguttata); arctidae (Arctidae) such as fall webworm (Hyphantriacanea); noctuidae (Noctuidae) such as Trichoplusia ni (trichoplusiani), Trichoplusia brassicae (mamestrabarasica), Spodoptera exigua (spodopterageua), Spodoptera litura (spodoptera litura), Heliothis armigera (heliotropigeria), Oriental armyworm (pseudolitea seprate), and Tiger (Agrotis ipsilon); tortricidae (Tortricidae) such as tea leaf moth (adoxyphesoranafiasta), apricot leaf roller moth (Archipsfussciureus), tea leaf roller moth (homoamagnanima); diamondback moth (Plutellidae) such as diamondback moth (plutella xylostella); the family of the Gelechiaceae (Gelechiaceae) such as Helicoverpa armigera (petitophopogossypiella), Helicoverpa zea (Pink bollworm);

hemipteran pests, for example: such as Aphis (Aphididae), Myzus globulus (Adelgidae) or Phymatoviridae (Phyloxeridae), such as Aphis tabaci (Myzus persicae), Aphis gossypii (Aphis gossypii), Aphis nigricans (Aphis fabae), Aphis zeae (Aphis maidis) (corn leaf aphid), Aphis sativus (Acyrosipon pisum), Aphis solanacearundinacea (Aulacorthum solani), Aphis fabarus (Aphis crispatus), Mylophora major (Macrosiphumus elongata), Aphis graminicola (Macrosicola), Aphis graminearum (Macrosiphilus avenae), Aphis virens (Macrophilus auranticola), Aphis citricola (Rhodophytum dimorpha), Aphis citricola (Rhorosioides), Aphis gramineus (Bryophyllum grandis), Aphis virens (Bryophyllum sibiricus), Aphis japonica (Aphis japonica), Aphis maculata (Aphis viridae), Aphis viridae (Aphis sativae), Aphis viridae (Aphis viridae), Aphis viridae (Aphis viridae), Aphis viridae (Aphis viridae), Aphis viridae (Aphis viridae), Aphis viridae (Aphis viridae), Aphis viridae (Aphis viridae), and Aphis (Aphis viridae), Aphis viride), Aphis viridae), Aphis viride), Aphis viridae (Aphis viride), Aphis (Aphis viride), and Aphis viride), Aphis viride (Aphis viride), Aphis citri, Aphis (Aphis citri; cicadae (Cicadellidae), such as Nephotettix cincticeps (Nephoteix cincticeps); delphacidae (Delphacidae), such as Laodelphax striatellus (Laodelphax striatellus), Nilaparvata lugens (Nilaparvata lugens) and Sogatella furcifera (soratella furcifera); stinkbug (pentanamidae), such as stinkbugs niveus (Eysarcoris ventralis), lygus lucorum (Nezara viridula); whitefly families (Aleyrodidae), such as Bemisia tabaci (Bemisia tabaci) and Trialeurodes greenhouse (Trialeurodes vaporariorum); pellegridae (Diaspididae), arachnidae (Margarodidae), Stachyridae (Orthezidae), Aclerdiane, Pachyridae (Dactylopoidae), Geckiaceae (Kerridae), and Pseudococcidae (Pseudiodidae); lecanidae (cocccidae); lecanidae (Eriococcudae); the family of the Lecanidae (Asterolecaniidae), the family of the Lecanispidae (Lecanispididae); or the family of the coccidae (Cerococcidae), such as the family of the Ericerus constellae (Pseudococcus comstocki) and the family of the Megasterus citri Risso (Planococus citri); psyllidae (Psyllidae) such as psyllid (psyllicularia), diaphorismata (diaphorinatabaaci);

coleopteran pests, for example: rice weevil (ostrinia oryzae) citrus tenella (potamuricomelanicolis chem), maize weevil (s. zeamais), cereal weevil (s. granatus), gibbonella ape (cabberella), ape leaf worm (Daikon leaf beetle), flea beetle (fleabeetle), grape flea beetle (allicarambola), striped flea beetle (Phyllotreta), cucumber flea beetle (epixcucuucus), tobacco flea beetle (erirtipenseria), leaf beetle (e. fusculula), yellow melon (audionida) (gemmollin), leaf beetle mustard (phaedoncholia), rice water weevil (lissoricullus oryzae), calophylla, yellow melon (yellow potato), yellow rice leaf beetle (yellowhorn), yellow melon (yellowhorn), yellow rice leaf beetle (yellowhorn leaf beetle), yellow melon (yellow rice leaf beetle (yellowhorn leaf beetle), red beetle (yellow beetle), yellow beetle (yellowhorn leaf beetle (yellow beetle, yellow;

dipteran pests, for example: rice plant fly (agromozaoryza), barley water fly (hydralligriseola), gerbera camomilla (liriomyzatrifolii), pea leaf fly (chromoniahorticola), tomato leaf fly (liriomyzabryoniae), gray fly (delactatura), green onion fly (delavata) mediterranean fly (ceratitis capitata wiedman), apple fly (rhagoletisponella), cherry fly (r.

Coleoptera (Coleoptera) pests, such as weevil (Lissorhoptrus oryzae), callosobruchus chinensis, Tenebrio molitor (Tenebrio molitor), Diabrotica virgifera (Diabrotica virgifera virgifera), cucurbita sativa (Diabrotica undecimentis howard di), verdigris (Anomala cuora), red copper tortoise (Anomala rufouchaeta), phylla flavus (phyllotryta), yellow melon (Anomala), potato beetle (leptinotara), rice mud worm (Oulema oryzae), bostrychia and bokedaceae (ceraceae);

insects of the order Acarina (Arachnida). For example, panonychus citri (pannychus urtri), tetranychus cinnabarinus (tetranychus nubilarinus), panonychus ulmi (pannychus ulmi), tetranychus urticae (tetranychus urticae), spider mites (oligochyrhinus) and phyllopodium citrosum (eotranychus pratenskias), red short-spotted spider mites (breviperuricis), alfalfa mites (bryopyratia), panonychus tritici (eriophyceae), vitiligo chinensis (acarit), vitiligo sinensis (acarus carinatus), citrus rust (phyllopodium album), yellow mite (giganticus), phylloxera giganteus (acarus purpureus), phyllopodium giganteus (acarus macrorrhiza), phyllopodium purpurea (phyllorhiza scrophus), phyllorhiza scrophularia (acae), phyllotrypanopyrtus purpurea (acarus macrorrhiza), phyllum giganteus (acarus, phyllanthus striatus, phyllorhii (acarus purpurea sinensis), phyllotrypannicus (purpurea, etc.;

thysanoptera (thysanoptera) pests, such as Thrips palmi (Thrips palmi), Thrips occidentalis (Frankliniella occidentalis), Thrips tabaci (Prisner), Thrips palmi (Thrips hawaiensis (Morgan), Thrips tea (Scirtothrips dorsals Hood), Thrips floribunda (Frankliniella intonsa (Trybom), etc.;

orthopteran (Orthopteran) pests, such as Acrididae (Acrididae); stinkbug (megatiptatticimum), corium elephatis (eurydemasum), stinkbug (eusartorius major), stinkbug (eusarcocylilissis), stinkbug (eusarcocorisparvus), stinkbug (nezaraviridula), stinkbug (platistali), stinkbug (halirphista), stinkbug (clethostachydigitalis), stinkbug (clethostus punctiger), stinkbug (lygus lucentis), stinkbug (leptospiensis), stinkbug (sterrisitis), and stinkbug (gonotylupestium).

Hymenoptera (hymenoptera) pests such as yellow winged wasp (athaliarosaerficornis), rose leaf wasp (argepagana), black ant (formica japonica), chestnut gall wasp (dryocsumuriphilus), and the like.

Isopoda (Isopoda), for example, Gardenia jasminoides Ellis (Oniscussellus), Armadillidium vulgare (Armadillidium vulgare), Armadillidium globosum (Porcellioscaber).

Diplopoda pests, for example, Blaniulus guttulatus.

Pest species of the order Labiatae, for example, Geophilus spp.

General purpose pests, for example, white pine (scottinellaena).

Thysanura pests, for example, Chlamydomonas (Lepismasaccharina).

Insects of the order Rhynchophylla, for example, Onychiurus armatus (Onychiurus armatus).

Orthoptera pests, for example, cricket-house (Acheta species), Phillips species (Gryllotalpa spp.), Asiatic migratory locusts (Loustamigratory migrarioids), Black locusts species (Melanoplus pp), desert locusts (Schistocercagregaria).

Pests of the order Blattaria, for example, Blatta orientalis (Blatta orientalis), Periplaneta americana (Periplaneta americana), Blatta maderae (Leucophaeamaderma), Blattella germanica (Blattegera).

Dermaptera pests, for example, Forficula auricularia (Forficula auricularia).

Isoptera pests, for example, the Reticulitermes spp.

From the order of the louse (Anoplura, Phthiraptera), for example, the genera zoophthiridae (Damalinia spp.), pediculophthiridae (Haematopinus spp.), pediculopterus (Linogaphus spp.), pediculosis (Pediculus spp.), and pediculosis cuckii (Trichodectes spp.).

Representative organisms of the class Nematoda (Nematoda) are selected from the group consisting of Meloidogyne spp, Heterodera spp, Globodera spp, Heterodera spp, Brevibacterium spp, and Longidorus spp. Preferred are soybean cyst nematode (Heterodera), potato nematode (Golbodera roseochiensis), Meloidogyne incognita (Meloidogyne incognita), and the like, which are the following nematodes.

The pesticidal composition of the present invention is preferably particularly effective against lepidopteran, hemipteran, dipteran, thysanoptera, acarina pests.

In particular, the pesticidal composition of the present invention is particularly effective against the following pests; specifically, the following pests are included:

noctuidae (Noctuidae) such as Trichoplusia ni (trichoplusiani), Trichoplusia brassicae (mamestrabarasica), Spodoptera exigua (spodopterageua), Spodoptera litura (spodoptera litura), Heliothis armigera (heliotropigeria), Oriental armyworm (pseudolitea seprate), and Tiger (Agrotis ipsilon);

tortricidae (Tortricidae) such as tea leaf moth (adoxyphesoranafiasta), apricot leaf roller moth (Archipsfussciureus), tea leaf roller moth (homoamagnanima);

diamondback moth (Plutellidae) such as diamondback moth (plutella xylostella);

arctidae (Arctidae) such as fall webworm (Hyphantriacanea);

whitefly families such as cabbage whitefly (Aleurodes brassicae), Bemisia tabaci (bemis tabaci), greenhouse whitefly (Trialeurodes vaporariorum);

delphacidae (Delphacidae), such as Laodelphax striatellus (Laodelphax striatellus), Nilaparvata lugens (Nilaparvata lugens) and Sogatella furcifera (soratella furcifera);

tetranychidae (Tetranyhidae) such as Tetranychus cinnabarinus (Tetranyhuscidinabarinus), Tetranychus urticae (Tetranyhusuricae), Tetranychus viennensis (Tetranyhusviensis), Tetranychus urticae (oligonyhusunhunguus), and Tetranychus citrullus (eotarenturanychus ankanus);

panonychus family (Panonychus spp.) such as Panonychus citri (pannyhusci), Panonychus ulmi (pannyhusulli);

gall mites (Eriophyidae) such as vitis vinifera (colemerrus vitis), camellia sinensis (calaruscarinatus), citrus aurantiaca (Phyllocoptes oleivora Ashmead), citrus oncophytes (aceerasilconi), spicules aculeatus (aculuschingendili), and the like;

thrips (Thripidae) such as Thrips palmi (Thrips palmi), Thrips occidentalis (Frankliniella occidentalis), Thrips tabaci (Prisner), Thrips palmi (Thrips aquifolium), Thrips palmi (Morgan), Thrips tea (scirthips dorsalis Hood), Thrips floribunda (Frankliniella intensa (Trybom), etc.;

in particular, the pesticidal compositions of the present invention exhibit an amazing "synergistic" effect on the prevention or control of diamondback moth, noctuidae, lampwick, planthopper, whitefly, tetranychidae, gall mite, thrips and their larvae and eggs.

The pesticidal compositions according to the invention surprisingly exhibit very high activity in the treatment of plants, propagation material of plants, soil in which plants grow, in the prevention or control of pests of the families plutellae, noctuidae, lampridae, planthopper, whitefly, tetranychidae, gall-mite, thrips and their larvae and eggs.

The insecticidal composition of the present invention has excellent control efficacy against plutella, noctuidae, lamplight moth, planthopper, whitefly, tetranychidae, gall mite, thrips and their larvae and eggs at low doses.

In another aspect, the invention also provides the use of an insecticidal composition for the prevention or control of diamondback moth, noctuidae, lampridae, planthopper, whitefly, tetranychidae, gall mite, thrips and larvae and eggs thereof.

The invention provides an application of an insecticidal composition in preventing or controlling vermin such as carmine spider mite, rust tick, bemisia tabaci, whitefly, diamond back moth, prodenia litura, fall webworm, meadow greenfly, beet armyworm, laodelphax striatellus, sogatella furcifera, thrips and the like, and larvae and eggs thereof.

The pesticidal compositions of the present invention may be applied to any and all developmental stages of a pest, such as eggs, larvae, pupae and adults. Pests can be controlled by contacting the target pest, its food supply, habitat, breeding grounds or their locus with a pesticidally effective amount of the pesticidal composition of the present invention.

"locus" refers to a plant, plant propagation material, soil, area, material or environment in which pests are growing or may grow.

"pesticidally effective amount" refers to the amount of the pesticidal composition of the present invention required to obtain an observable effect on growth, including a mortality, prophylactic or removal effect, a destructive effect or an effect that reduces the appearance and activity of animal pests. The pesticidally effective amount of each pesticidal composition used in the present invention may vary. The pesticidally effective amount of the composition will also vary depending on the prevailing conditions such as the desired pesticidal effect and duration, the weather, the target species, the locus, the mode of application and the like.

All plants and plant parts can be treated according to the invention. "plants" is understood here to mean all plants and plant populations such as desired and undesired wild plants or crops (including naturally occurring crops). Crops may be plants obtained by conventional breeding and optimization methods, or plants obtained by biotechnological and genetic engineering methods or combinations of these methods, including transgenic plants and including plant cultivars which may or may not be protected by plant breeders' certificates. Plant parts are understood to mean all parts and plant organs above and below the ground, such as shoots, leaves, flowers and roots, examples which may be mentioned being leaves, needles, stems, flowers, fruit bodies, fruits and seeds and roots, tubers and rhizomes. Parts of plants also include harvested plants and vegetative and generative propagation material, for example seedlings, tubers, rhizomes, cuttings and seeds.

As mentioned above, all plants and parts thereof can be treated according to the invention. According to the invention, it is particularly preferred to treat plants of the plant cultivars which are in each case commercially available or in use.

The pesticidal composition of the present invention shows particularly advantageous effects when used for, for example, the following plants:

crops: corn, rice, wheat, barley, rye, oat, sorghum, cotton, soybean, peanut, buckwheat, beet, brassica oleracea, cabbage, rapeseed, sunflower, sugarcane, tobacco, and the like.

Vegetable: solanaceae vegetables (eggplant, tomato, green pepper, hot pepper, potato, etc.), cucurbitaceae vegetables (cucumber, pumpkin, zucchini, watermelon, melon, etc.), brassicaceae vegetables (rape, radish, turnip, horseradish, kohlrabi, cabbage, mustard, hard cauliflower, etc.), compositae vegetables (burdock, artemisia, artichoke, lettuce, etc.), liliaceae vegetables (shallot, onion, garlic, asparagus, etc.), cymbidium vegetables (carrot, parsley, celery, parsnip, etc.), chenopodiaceae vegetables (spinach, swiss chard, etc.), labiatae vegetables (perilla frutescens, mint, basil, etc.), strawberry, sweet potato, taro, etc.

Fruit trees: pome fruits (apple, avocado, japanese pear, papaya), stone fruits (peach, plum, nectarine, plum, yellow peach, apricot, prune, etc.), citrus (satsuma mandarin, orange, lemon, lime, grapefruit, etc.), nuts (chestnut, walnut, hazelnut, almond, cashew, etc.), berry fruits (raspberry, blackberry), grape, persimmon, olive, loquat, banana, coffee, date, coconut, oil coconut.

Trees other than fruit trees: tea, mulberry, flowering trees (azalea, camellia, hydrangea, camellia oleifera, japanese anise, cherry, crape myrtle, cinnamon, etc.), street trees (birch, ginkgo, clove, maple, oak, poplar, cercis, sweetgum, sycamore, zelkova, japanese cedar, fir, japanese hemlock, juniper, pine, spruce, yew, elm, etc.), coral tree, podocarpus macrophyllus, cedar, japanese cypress, croton, photinia glabra, etc.

Lawn: zoysia (zoysia japonica, zoysia sulcata, etc.), bermuda grass (bermudagrass, etc.), agrostis (giant-order agrostis, creeping agrostis, dry-land agrostis, etc.), bluegrass (meadow bluegrass, bird grass, etc.), fescue (festuca arundinacea, red fescue, etc.), ryegrass (poison wheat, ryegrass, etc.), orchard grass, timothy grass, etc.

And others: flowers (roses, dianthus caryophyllus, chrysanthemum, platycodon grandiflorum, dianthus chinensis, gerbera, calendula, salvia, petunia, verbena, tulip, gentian, lily, trichlorfon, cyclamen, orchid, lily, lavender, violet, kale, primula, euphorbia, daisy, cymbidium, etc.), biofuel plants (leprosy, safflower, arundina, switchgrass, miscanthus, reed canary grass, bamboo, kenaf, cassava, willow, etc.), ornamental plants, and the like.

The pesticidal composition of the present invention has good plant compatibility and is suitable for controlling pests, particularly insects (Insecta), arachnids (Arachnida), nematodes (Nematoda), and fungi, rodents, microorganisms and the like; in particular diamondback moth, noctuidae, lamplight moth, planthopper, whitefly, tetranychidae, gall mite, thrips and their larvae and eggs. It is preferably applicable to foliar treatment, soil treatment and seed treatment.

In another aspect, the invention provides a method for preventing or controlling pests and their larvae and eggs by applying the pesticidal composition of the invention to a target useful plant, a target pest or its environment, propagation material of a target useful plant. This can be done before and after the plant, plant propagation material or environment is infested with pests.

The term "plant propagation material" is understood to mean all reproductively competent plant parts, such as seeds, which can be used for the propagation of the latter, and also vegetative materials, such as cuttings or tubers (e.g. potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.

The plant propagation material is a seedling, rhizome, nursery seedling, cutting or seed. Seeds are preferred.

The pesticidal composition according to the present invention may be applied to seeds, plants or fruits of plants or soil where plants grow or soil suitable for plant growth.

A method of protecting seeds comprising contacting the seeds before sowing and/or after pregermination with a pesticidally effective amount of the pesticidal composition of the invention.

The plants may include: stems and leaves of plants, flowers of plants, fruits of plants, and the like.

The seed is selected from soybean, wheat, barley, rye, sorghum, peanut, sunflower, sugarcane, tobacco, rice, rape, cole, cabbage, beet, tomato, cowpea, carrot, cotton and corn seed.

Accordingly, the present invention provides a method for protecting seeds, seedlings roots and shoots from attack by soil and foliar insects comprising contacting the seeds prior to sowing and/or after pregermination with an effective amount of the pesticidal composition of the present invention.

The invention also provides a method of controlling or preventing pests and their larvae and eggs in plant propagation material and plant organs, plant parts and/or plants which grow at a later time, which comprises applying the pesticidal composition of the invention to the plant propagation material or its environment. The plant propagation material is preferably a seed.

Most of the damage caused by pests to crop plants occurs as early as when the seeds are infested, during storage and after the seeds are sown into the soil and during or after germination of the plants. This stage is particularly critical because the roots and shoots of growing plants are particularly sensitive, and even minor damage can lead to death of the entire plant. Therefore, the protection of seeds and germinating plants by using suitable compositions is of particular interest.

The invention also relates in particular to a method for protecting seeds and germinating plants from attack by pests and their larvae and eggs by treating the seeds with the insecticidal composition according to the invention. The present invention relates to a method for protecting seeds and germinating plants from the attack of plutella, noctuidae, lampwick, planthopper, whitefly, tetranychidae, gall-mite, thrips and their larvae and eggs, comprising the simultaneous treatment of said seeds with compound I and compound II. It also includes a method of treating the seed with compound I and compound II at different times.

A method for preventing or controlling pests and their larvae and eggs, by treating the seeds of the plants to be grown before sowing and/or after pregermination with a synergistically effective amount of the pesticidal composition according to the invention.

The invention also relates to the use of the pesticidal composition of the invention for treating seeds to protect said seeds and plants grown therefrom from attack by pests and their larvae and eggs.

Furthermore, the invention relates to seeds treated with the pesticidal composition of the invention to obtain protection against pests and their larvae and eggs.

The invention also relates to seeds treated simultaneously with compound I and compound II. The invention also relates to seeds treated with compound I and compound II at different times. For seeds treated with compound I and compound II at different times, the respective active compounds of the pesticidal composition of the present invention may be present in different layers on said seeds. Wherein the layers comprising compound I and compound II are optionally separated by an intermediate layer. The invention also relates to seeds wherein compound I and compound II are applied as coating components or as one or more additional layers outside the coating.

The pesticidal composition of the present invention may be applied to seeds in any physiological state. Preferably, the seeds are in a sufficiently durable state so as not to be damaged during handling. Generally, the seeds may be seeds harvested from the field, removed from the plant, isolated from any cob, stem, husk and surrounding pulp or other non-seed plant material. The seed may also preferably be biologically stable to the extent that the treatment does not cause biological damage to the seed. The treatment applied to the seed may be between seed harvest and seed sowing or at any time during the sowing process. The seeds may also be pregerminated before or after treatment.

The seeds treated with the pesticidal composition of the present invention can be stored, managed, sowed and tilled.

Seed treatment occurs on unsown seeds, and the term "unsown seeds" is intended to include seeds at any time between harvest of the seeds and sowing of the seeds in the ground for the purpose of germination and growth of the plant. Preferably, the treatment occurs before sowing of the seeds, whereby the sown seeds have been pre-treated with the combination. In particular, seed coating or seed pelleting is preferred in the treatment of the combination of the invention. After treatment, the components of each combination adhere to the seed and are thus available for pest control.

Seed treatment methods include all methods known to the person skilled in the art which are suitable for treating seeds, such as seed dressing, seed coating, seed soaking, seed coating, seed multilayer coating, seed encrusting, seed wetting, seed dusting and seed pelleting.

The compositions of the present invention may be applied to seeds, plants or fruits of plants or soil where plants are growing or soil suitable for plant growth.

The present invention also provides a method for preventing or controlling pests and their larvae and eggs, comprising applying the pesticidal composition of the present invention to seeds, target useful plants or soil in which plants grow or soil suitable for plant growth.

In another aspect, the invention also provides a method for preventing or controlling pests and their larvae and eggs by applying the pesticidal composition of the invention to the soil before, after or before germination of the seeds and/or directly to the soil in contact with the roots of the plants or to the soil suitable for the growth of the plants.

The invention also provides a method of protecting plants from attack by pests and their larvae and eggs, which comprises applying the pesticidal composition of the invention to the environment, habitat or storage area where useful plants are growing. The environment and habitat for plant growth refers to a support capable of rooting and growing crops, such as: examples of the raw material include sand, pumice, vermiculite, diatomaceous earth, agar, gel, polymer, asbestos, wood chips, and bark. Preferably soil.

The invention also provides a method for protecting plants against infestation by pests of the families plutellae, noctuidae, lampwick, planthopper, aleyrodidae, tetranychidae, gall-mites, thrips and their larvae and eggs, which comprises applying the active ingredients compound I and compound II jointly, separately or in succession. In the case of separate application, the sequence usually has no effect on the results of the control measures.

A method for preventing or controlling pests and their larvae and eggs, comprising applying compound I, compound II separately, sequentially or simultaneously.

The invention also provides a method for protecting useful plants against attack by pests and their larvae and eggs, which comprises applying a combination comprising compound I and compound II, in any desired sequence or simultaneously, on the target useful plant or its environment, the target pest or its environment, propagation material of the target useful plant.

The insecticidal compositions of the present invention include not only ready-to-use compositions which can be applied to plants or plant propagation material with a suitable device, but also commercial concentrates which must be diluted with water prior to application.

The insecticidal composition of the present invention may be applied undiluted or diluted with water.

The present invention provides a method for preventing or controlling pests and their larvae and eggs, by applying the pesticidal composition of the present invention to the target useful plants or their environment, the propagation material of the target useful plants, the other pesticides can be applied before, simultaneously with or independently of compound I and compound II, and in the case of separate application of compound I and compound II, each other pesticide can be applied before, simultaneously with, between or independently of compound I and compound II.

The application rate is adjusted by the person skilled in the art according to the actual circumstances and needs, in particular according to the nature of the plants or crops to be treated and the pest situation.

Methods for treating target pests, target useful plants, or seeds, soil with the pesticidal composition of the present invention include, for example, spreading treatment, soil treatment, surface treatment, and fumigation treatment. Spreading treatments include, for example, spreading, spraying, atomizing, particle application. Soil treatment includes, for example, soil irrigation and soil mixing. Surface treatments include, for example, coating, covering. Fumigation treatments include, for example, covering the soil with a polyethylene film after injection into the soil.

Applicators typically use the insecticidal compositions of the present invention in a backpack sprayer, spray can, spray plane or irrigation system. The pesticidal compositions of the present invention are typically formulated with water, buffering agents and/or other adjuvants to the desired application concentration to give a ready-to-use spray.

The total amount of active ingredients in the pesticidal compositions of the present invention may be selected to achieve the desired effect, depending on the particular factors. Such as dosage form, subject to be administered, method of administration, and the like.

The invention provides an insecticidal composition, which contains active ingredients of a compound I and a compound II, wherein the weight of the compound I and the compound II accounts for 1% -90%, preferably 5% -80%, more preferably 5% -70%, still more preferably 5% -60%, and more preferably 10% -60% of the weight of the insecticidal composition.

A pesticidal composition, which contains as active ingredients compound I and compound II, the amounts by weight of compound I and compound II together also amounting to 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% by weight of the pesticidal composition, for example.

The pesticidal composition of the present invention may optionally comprise auxiliary ingredients such as agriculturally acceptable surfactants and/or fillers. Preferably, the auxiliary component is one or a mixture of a dispersant, a wetting agent, an antifreeze agent, a thickening agent, an antifoaming agent, a disintegrating agent, a binder and an auxiliary carrier.

According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g. plants, crops or grasses). Thus, the bulking agent is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.

The inactive fillers that can be used in the present invention can be either solid or liquid.

Examples of solid fillers that can be used are: talc, bentonite, clay, kaolin, diatomaceous earth, white carbon black, calcium carbonate, acid clay, silica, zeolite, attapulgite, pumice, ammonium sulfate, sodium sulfate, and urea.

The liquid filler that can be used may be selected from water, isopropyl alcohol, butyl alcohol, tetrahydrofuran, ethylene glycol, propylene glycol, ethyl acetate, methyl oleate, dimethylformamide, dimethylacetamide, dimethylsulfoxide, vegetable oil, and the like.

Surfactants which can be used for emulsifying, dispersing, solubilizing and/or wetting the active ingredient compounds include, for example, fatty alcohol polyoxyethylene ethers, polyoxyethylene alkylaryl ethers, polyoxyethylene higher fatty acid esters, phosphoric acid esters of polyoxyethylene alcohols or phenols, fatty acid esters of polyhydric alcohols, naphthalenesulfonic acid polymers, lignosulfonates, comb-like copolymers of high molecules, butylnaphthalenesulfonates, alkylarylsulfonates, alkylsulfosuccinates, fats and oils, condensates of fatty alcohols with ethylene oxide, polyacrylates of alkylnaphthalenesulfonates, protein hydrolysates, suitable oligosaccharides or polymers, based, for example, on vinyl monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.

For dispersing, stabilizing and adhering the active ingredient compounds, auxiliary agents such as xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose methyl ether, polyvinyl alcohol, polyvinyl acetate, natural phospholipids (such as cephalin and lecithin), synthetic phospholipids, bentonite, sodium lignin sulfonate and the like can be used.

Wherein the antifreezing agent can be selected from ethylene glycol, propylene glycol, glycerol, and sorbitol. As the deflocculant for the suspendable product, an auxiliary such as a naphthalenesulfonic acid polymer, a polymeric phosphate, or the like can be used. As the defoaming agent, a silicone defoaming agent can be used. In order to improve the flowability of the solid product, auxiliaries such as paraffin, stearate, alkyl phosphate and the like can be used.

Colorants which may be used, for example, inorganic pigments such as iron oxide, titanium oxide and prussian blue; and organic pigments/dyes, such as alizarin dyes, azo dyes, and metal phthalocyanine dyes; and trace elements such as iron, manganese, boron, copper, cobalt, molybdenum and zinc salts.

Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.

The pesticidal composition according to the invention can be used as such or in the form of its formulation or in the use form prepared therefrom according to its respective physical and/or chemical properties, such as aerosols, microcapsule suspensions, cold fogging formulations, hot fogging formulations, microcapsule granules, fine granules, suspensions for seed treatment, ready-to-use solutions, powders, emulsifiable concentrates, oil-in-water emulsions, water-in-oil emulsions, macrogranules, microgranules, oil-dispersible powders, oil suspensions, oil solutions, foams, pastes, seed coatings, colloidal suspensions, suspoemulsions, aqueous solvents, suspensions, wettable powders, soluble powders, dusts and granules, water-soluble granules or tablets, water-soluble powders for seed treatment, wettable powders, natural and synthetic substances impregnated with active ingredients, microcapsules in polymeric materials and seed-coating substances, and ultra-low-volume cold fogging and hot fogging formulations.

The preferred preparation formulation of the insecticidal composition provided by the invention is missible oil, water suspending agent, oil suspending agent, seed treatment dry powder, seed treatment suspending agent, water dispersible granule, wettable powder, suspoemulsion, aqueous emulsion, microcapsule suspending agent, dry suspending agent, ultra-low volume liquid, microgranule and microemulsion.

The pesticidal compositions of the present invention may also be applied in combination with other active ingredients such as fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals and the like.

The formulations according to the invention can be prepared by mixing the active ingredients with the customary additives in a known manner. Such as conventional extenders as well as solvents or diluents, emulsifiers, dispersants, and/or binders or fixatives, wetting agents, water repellents, if desired siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.

The pesticidal composition of the present invention includes not only a composition which can be applied to a subject to be treated immediately by means of a suitable apparatus such as a spraying or dusting apparatus, but also a concentrated commercial composition which is diluted before application to the subject.

The insecticidal composition of the present invention can be prepared into a formulation suitable for application in the form of a combination of its active ingredient compounds, or can be applied in the form of a combination of commercial formulations in appropriate dosages.

Formulation examples

EXAMPLE 1 wettable powder

Compound I5%

Lufenuron 20%

Naphthalene sulfonate 3%

Sodium lignosulfonate 6%

Alkylphenol polyoxyethylene ether phosphate 5%

White carbon black 5%

Kaolin is complemented to 100%

The components are mixed according to a proportion, ground and crushed to prepare the wettable powder.

EXAMPLE 2 aqueous suspension

Compound I10%

Lufenuron 10%

Sodium fatty alcohol Ether sulfate 3%

Calcium dodecyl benzene sulfonate 3%

1% aqueous xanthan gum solution 2%

1 percent of bentonite

Propylene glycol 5%

0.05 percent of polysiloxane antifoaming agent

The water is complemented to 100 percent

All ingredients except the 2% aqueous xanthan gum solution were pre-mixed together with an appropriate amount of water and the mixture was then milled through a wet mill. Finally, a 2% aqueous xanthan gum solution and the remaining water were added to the ground product to prepare an aqueous suspension.

Example 3 Water dispersible granule

Compound I10%

Lufenuron 30%

10 percent of modified sodium lignosulfonate

Sodium dodecyl naphthalene sulfonate 4%

6 percent of ammonium sulfate

Talcum powder 5%

The diatomite is complemented to 100 percent

The components are uniformly mixed and ground, a certain amount of water is added for mixing, extruding and material making, and the water dispersible granules are obtained after drying and screening.

EXAMPLE 4 aqueous suspension

Compound I10%

Lufenuron 10%

Polyoxyethylene styryl phenyl ether 3%

Sodium alkylnaphthalenesulfonate formaldehyde condensate 3%

1% aqueous solution of xanthan gum 1%

0.5 percent of bentonite

Propylene glycol 7%

0.03 percent of polysiloxane antifoaming agent

The water is complemented to 100 percent

All ingredients except the 1% aqueous xanthan gum solution and the appropriate amount of water were pre-mixed together and the mixture was then milled through a wet mill. Finally, a 1% aqueous xanthan gum solution and the remaining water were added to the ground product to prepare an aqueous suspension.

Example 5 emulsifiable concentrate

Compound I1%

5 percent of lufenuron

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

SOLVESSO^TM200 to 100%

The components are mixed and stirred evenly to prepare missible oil.

Example 6 suspoemulsion

Compound I10%

5 percent of lufenuron

Oleic acid methyl ester 10%

Ethoxylated castor oil 4%

3 percent of disodium fatty alcohol polyoxyethylene ether sulfosuccinate monoester

3 percent of modified calcium lignosulfonate

1% aqueous solution of xanthan gum 1%

1 percent of bentonite

Glycerol 5%

The water is complemented to 100 percent

Dissolving lufenuron in methyl oleate, adding ethoxylated castor oil, and modifying calcium lignosulfonate to obtain lufenuron emulsifiable concentrate.

The compound I, the disodium fatty alcohol polyoxyethylene ether sulfosuccinate monoester and the other components are uniformly mixed according to the proportion and are sanded to prepare the suspending agent containing the compound I.

Adding the oil phase containing lufenuron into the suspending agent containing the compound I to obtain the suspending emulsion.

The above proportion is weight percentage.

Biological test example

The expected effect of a particular combination of two active ingredients can be calculated using the so-called "Colby' formula" (see s.r. Colby, "marketing synergy and antibiotic Responses of pharmaceutical compositions", Weeds 1967,15, 20-22):

x is the control effect when using the active ingredient compound of the formula I in an amount of m g/ha or at a concentration of m ppm, expressed as a percentage of the untreated control,

y is the control effect when active ingredient compound II is used in an amount of n g/ha or at a concentration of n ppm, expressed as a percentage of the untreated control,

e is the control effect when using the active ingredients compounds of the formula I in amounts of m and n g/ha or concentrations of m and n ppm and lufenuron, expressed as a percentage of the untreated control,

if the actual control effect exceeds the calculated value, the lethal effect of the composition is super-additive, i.e. synergistic effect exists.

Test example 1 diamondback moth test

Preparing a liquid medicine: preparing compound I and lufenuron into mother liquor with acetone, and diluting with 0.1% Tween-80 aqueous solution to the required concentration.

Selecting 2-instar diamondback moth larvae which are continuously raised indoors and have consistent physiological state. Selecting cabbage leaves with consistent growth to prepare a proper leaf disc.

Soaking the leaf disc in the solution of the reagent to be tested, taking out the leaf disc after 10s, airing the leaf disc, placing the leaf disc in a culture dish containing moisturizing filter paper, and inoculating the test insects. About 10 test insects are inoculated in each group of test. Each treatment was repeated 4 times. And a treatment without a chemical agent (containing an organic solvent and an emulsifier) was set as a blank control.

Adding the treated test insects after covering the test insects, and placing the test insects at a position of 25 +/-1^oC, relative humidity of 60% -80%, illumination period L: d = (16:8) h of constant temperature incubator feeding and observation.

The results were checked after 48h in the incubator. The death judgment criteria were: the body of the worm is touched with a poking needle, and the worm body is dead without reaction or can not react normally.

In the test process, the prepared liquid medicine is accurate, and the error is reduced; when in operation, the user needs to be skilled and quick, and the test operation specification is paid attention to, so that the test accuracy is ensured. The blank test should be repeated when the mortality rate exceeds 20%.

The calculation method comprises the following steps:

mortality (%) = (number of living insects before medicine-number of living insects after medicine)/number of living insects before medicine x 100

Control effect% = ((treatment group mortality-control group mortality)/(100-control group mortality)) × 100

TABLE 1 Effect of the combination of Compound I and lufenuron on Plutella xylostella

Test example 2 thrips test

Preparing a liquid medicine: preparing the compound I and lufenuron raw materials into mother liquor by using acetone, and diluting the mother liquor to the required concentration by using 0.1% Tween-80 aqueous solution.

Thrips tabaci was used as the test pest. And (3) inoculating the collected thrips tabaci to the eggplant potted in the greenhouse for long-term breeding. Before the test, the tobacco thrips were selected by microscopic examination and bred to eggplant plants planted in the greenhouse (without other species of thrips). The breeding conditions are glass net roomHowever, with light, the period is about 16 (L): 8 (D). A maximum temperature of 28 deg.C^oC, minimum 15^oC, the humidity is 95% at most and 65% at most. F1 generation adults were selected for testing. The laboratory tests were carried out in groups of 20-40 heads. The procedure was carried out according to the Insectival method (NY/T1154.6-2006).

Cutting off the leaf of eggplant with the adult thrips to be tested, soaking in the liquid medicine for 5-10S, taking out, sucking the redundant liquid medicine on the leaf and the thrips with filter paper, transferring the thrips to fresh nontoxic eggplant tender leaves with a fine fluff pen, placing in a culture jar, covering, keeping ventilation and smoothness, and keeping humidity with a small wet cotton ball. Placing culture jar containing processed thrips in 25 + -1^oC, feeding and observing in a constant temperature incubator with the relative humidity of 60% -80%. Each treatment was repeated 4 times. And a treatment without a chemical agent (containing an organic solvent and an emulsifier) was set as a blank control.

The treatment is carried out for 24h, and the death condition of the test insects is investigated. Judging the death standard as that the soft fine hairbrush does not have obvious reaction crawling when being touched, and recording the total number of the insects and the number of the dead insects. And calculating the mortality and the control effect.

In the test process, the prepared liquid medicine is accurate, and the error is reduced; when in operation, the user needs to be skilled and quick, and the test operation specification is paid attention to, so that the test accuracy is ensured. The blank test should be repeated when the mortality rate exceeds 20%.

The calculation method comprises the following steps:

mortality (%) = number of dead test insects/number of test insects before drug × 100

Control effect% = ((treatment group mortality-control group mortality)/(100-control group mortality)) × 100

TABLE 2 combination of Compound I and lufenuron against thrips tabaci

Test 3 tomato Bemisia tabaci test

Preparing a liquid medicine: preparing the compound I and lufenuron raw materials into mother liquor by using acetone, and diluting the mother liquor to the required concentration by using 0.1% Tween-80 aqueous solution.

Before the test, bemisia tabaci was inoculated on greenhouse potted insect-free tomato seedlings planted in an insect-proof net room. When the eggs are laid and hatched to develop into nymphs of 2-3 generations of F1, tomato leaves are cut, and non-test nymphs such as eggs and the like are removed through microscopic examination, wherein each leaf test nymph has 30-50 heads for standby test.

Soaking bemisia tabaci and tomato leaves in the prepared liquid medicine for 5 seconds, taking out, sucking the redundant liquid medicine with absorbent paper, and putting into a plastic beaker with the diameter of 6cm and the height of 10 cm. After the test was completed, the test was transferred to 25. + -.1^oC, feeding in a constant temperature incubator with the relative humidity of 60% -80%. Each treatment was repeated 4 times. And a treatment without a chemical agent (containing an organic solvent and an emulsifier) was set as a blank control.

At 72h after treatment, the test insects were examined for death under a microscope. The death criteria are judged to be shriveled body and withered and yellow color of the worm. If the control mortality rate is more than 20%, the test is redone. Total and dead worm numbers were recorded. And calculating the mortality and the prevention and treatment effect.

In the test process, the prepared liquid medicine is accurate, and the error is reduced; when in operation, the user needs to be skilled and quick, and the test operation specification is paid attention to, so that the test accuracy is ensured.

The calculation method comprises the following steps:

mortality (%) = number of dead test insects/number of test insects before drug × 100

Control effect% = (treatment group mortality-control group mortality)/(100-control group mortality) × 100

TABLE 3 control of tomato Bemisia tabaci by combination of Compound I and lufenuron

Test 4 orange Russian test

Preparing a liquid medicine: preparing the compound I and lufenuron raw materials into mother liquor by using acetone, and diluting the mother liquor to the required concentration by using 0.1% Tween-80 aqueous solution.

The test was carried out by a membrane method. Taking a cylindrical transparent glass can, adding 50ml of the diluted liquid medicine with the required concentration, covering the cap tightly, shaking up gently, inverting for 30S to make the inner wall of the bottle fully adhered with the liquid medicine, pouring out the liquid medicine, and naturally drying the bottle.

Soaking tender shoots of citrus in the same medicinal liquid for 1min, naturally drying, and placing in an air-dried bottle; wrapping the tender shoot base with cotton soaked with the same liquid medicine for moisturizing. CO for adult plant louse₂After anesthesia (18-20S), the mixture is put into each treatment bottle. Each treatment was repeated 4 times, with 10 adults each time. And a treatment without a chemical agent (containing an organic solvent and an emulsifier) was set as a blank control.

Each bottle mouth is covered with a gauze to prevent adults from flying out. After 20 minutes, whether dead insects exist or not is checked, and the number of live insects before the pesticide is recorded. The glass bottle after receiving the insect is set to the temperature (25 +/-1)^oAnd C, in an illumination incubator. The number of dead insects and the number of live insects were visually observed 24 hours after the administration. And calculating the mortality and the prevention and treatment effect.

In the test process, the prepared liquid medicine is accurate, and the error is reduced; when in operation, the user needs to be skilled and quick, and the test operation specification is paid attention to, so that the test accuracy is ensured. The blank test should be repeated when the mortality rate exceeds 20%.

The calculation method comprises the following steps:

mortality (%) = number of dead test insects/number of test insects before drug × 100

Control effect% = (treatment group mortality-control group mortality)/(100-control group mortality) × 100

TABLE 4 combination of Compound I and lufenuron against ticks

The test results in tables 1-4 show that the combination of compound I and lufenuron gives unexpected synergistic effects on the control of diamond back moth, thrips, whitefly, and ticks.