阅读说明：本技术 一种集成纺织品超声提取及检测的微流控芯片 (Micro-fluidic chip integrating ultrasonic extraction and detection of textiles ) 是由 金迪琼 李倩 张梦帆 叶嘉明 于 2021-09-30 设计创作，主要内容包括：本发明提供了一种集成纺织品超声提取及检测的微流控芯片,微流控芯片包括通道层,通道层包括芯片基体和至少一个样品检测单元,样品检测单元位于芯片基体上,样品检测单元包括：超声提取池、微流体通道、分液池、检测池和通气孔,其中,超声提取池通过微流体通道与分液池连通,分液池通过微流体通道与检测池连通。超声提取池的上方有提取池盖,盖上有孔。使用时,打开提取池盖,放入样品和提取液,盖上提取池盖。本发明所提供的一种集成纺织品超声提取及检测的微流控芯片,将纺织品前处理与检测集成到一个系统中,避免了繁琐操作、人工误差,使纺织品质量安全检测更加方便,将极大改进纺织品中安全因子检测的时效性与准确性。(The invention provides a micro-fluidic chip for ultrasonic extraction and detection of integrated textiles, which comprises a channel layer, wherein the channel layer comprises a chip substrate and at least one sample detection unit, the sample detection unit is positioned on the chip substrate, and the sample detection unit comprises: the ultrasonic detection device comprises an ultrasonic extraction pool, a microfluidic channel, a liquid separation pool, a detection pool and a vent hole, wherein the ultrasonic extraction pool is communicated with the liquid separation pool through the microfluidic channel, and the liquid separation pool is communicated with the detection pool through the microfluidic channel. An extraction tank cover is arranged above the ultrasonic extraction tank, and a hole is arranged on the cover. When in use, the cover of the extraction tank is opened, the sample and the extracting solution are put in, and the cover of the extraction tank is closed. According to the micro-fluidic chip for integrated textile ultrasonic extraction and detection, textile pretreatment and detection are integrated into one system, so that complicated operation and manual errors are avoided, the quality safety detection of textiles is more convenient, and the timeliness and the accuracy of safety factor detection in textiles are greatly improved.)

1. A micro-fluidic chip for integrated textile ultrasonic extraction and detection is characterized by comprising a channel layer and a cover plate layer;

the channel layer comprises a chip substrate and at least one sample detection unit; the sample detection unit is positioned on the chip substrate; the sample detection unit includes: an ultrasonic extraction pool, a liquid separation pool, a detection pool and a microfluidic channel; the ultrasonic extraction pool is communicated with the liquid separation pool through a microfluidic channel, and the liquid separation pool is communicated with the detection pool through the microfluidic channel;

the cover plate layer is located above the channel layer, and an opening is formed in the cover plate layer and used for putting a sample to be tested into the ultrasonic extraction pool through the opening.

2. The microfluidic chip for integrated textile ultrasonic extraction and detection according to claim 1, wherein the cover plate layer comprises an extraction cell cover matched with the opening, the extraction cell cover is used for providing a channel inlet for putting a sample and an extraction solution to be detected into the ultrasonic extraction cell; and the extraction tank cover is provided with an extraction tank cover hole, and the extraction tank cover hole is used for gas-liquid circulation when the microfluidic chip is centrifuged.

3. The microfluidic chip for integrated textile ultrasonic extraction and detection according to claim 1, wherein the bottom of the ultrasonic extraction cell is in contact with an ultrasonic generator.

4. The microfluidic chip for integrated textile ultrasonic extraction and detection according to claim 1, wherein at least one liquid separation tank is arranged, the number of the liquid separation tanks corresponds to the number of the detection tanks, a textile quality safety detection reagent is pre-stored in each detection tank, and the detection tank area is made of a transparent material.

5. The integrated textile ultrasonic extraction and detection microfluidic chip according to claim 1, further comprising a central fixing hole, wherein the central fixing hole is located at the geometric center of the channel layer and the cover plate layer and overlapped to penetrate through the channel layer and the cover plate layer, and is used for being fixedly installed on a centrifugal detection device.

6. The microfluidic chip for integrated textile ultrasonic extraction and detection according to claim 1, wherein the reagent pre-stored in the detection cell comprises a formaldehyde detection reagent or a pH chromogenic reagent; the formaldehyde detection reagent is an alkaline phloroglucinol reagent; the pH detection reagent comprises one or two of bromocresol green, methyl red, methyl orange, indigo disulfonic acid sodium, bromophenol blue, alizarin sodium sulfonate, catechol violet, phenolsulfonphthalein, cresol red, bromothymol blue, m-cresol violet and naphthol benzyl alcohol.

7. The microfluidic chip for integrated textile ultrasonic extraction and detection according to claim 1, wherein the detection step of the microfluidic chip is as follows:

the method comprises the following steps: placing the textile in an ultrasonic extraction tank, adding an extracting solution, installing a microfluidic chip on centrifugal detection equipment, and setting a program by a control unit;

step two: ultrasonically extracting a textile extracting solution, adding the textile extracting solution into a sample detection pool through a centrifugal force or a sample injection unit, and reacting the textile extracting solution with a detection reagent prestored in the detection pool to obtain a reaction product;

step three: the detection pool is matched with the photoelectric detection mechanism, the collected information is sent to the control unit, and the control unit analyzes the information to obtain the content of the factors to be detected of the textile.

8. The microfluidic chip for integrated textile ultrasonic extraction and detection according to claim 7, wherein in the second step, when the microfluidic chip is a centrifugal chip, the microfluidic chip is mounted on a centrifugal device through a central fixing hole, and the centrifugal device is moved to drive the microfluidic chip to rotate, so that the textile extract in the ultrasonic extraction tank flows into the detection tank, and a corresponding reaction product is generated.

Technical Field

The invention relates to the technical field of textile detection, in particular to a micro-fluidic chip integrating textile ultrasonic extraction and detection.

Background

The textile is a necessity of daily life of people, and the quality of the textile is directly related to manufacturers, trade relatives, government supervision departments and each consumer. Pair spinning by each government supervision department

The quality of the fabrics is very important, and almost all countries issue mandatory standards of laws for protecting the legal rights and interests of consumers and stipulate numerous detection items of textiles. The influence of the textile sample pretreatment technology on the detection and analysis results occupies a great proportion, and analysis shows that: the error of the detection and analysis result is nearly 50% from the preparation and treatment of the sample, but is really from the analysis less than 30%, and the workload occupied by the pretreatment of most samples exceeds 70% of the whole analysis, so that the sample pretreatment system is the basis and guarantee for achieving the purpose of rapidness and accuracy of the detection system.

The detection technology of carcinogenic dye, sensitizing dye, pesticide and the like in the textile has a common characteristic: the substance to be detected is wrapped and deeply buried in the fiber and the fabric; meanwhile, with the continuous development of textiles, the textiles are not two simple components such as cotton, hemp or terylene, and more blended and interwoven textiles enter the market and become the leading component, so that the difficulty of the pretreatment of the textiles in detection is further increased. The pretreatment technologies at the present stage comprise solvent extraction, solid-phase micro-extraction, multi-element extraction and the like.

The ultrasonic extraction technology is formed by combining a solvent extraction technology and an ultrasonic technology, and the existence of an ultrasonic field improves the efficiency of solvent extraction. The paper published by Zhang Xiang et al in chromatographic 2007, 25 vol.3, the high performance liquid chromatography for simultaneously detecting 9 organochlorine pesticide residues in cotton fabrics, and the paper published by Wang Mingtai et al in printing and dyeing 2007, 8 th, the LCMS/MS (liquid Crystal display systems/Mass Spectrometry) method for organic nitrogen pesticide residues in textiles all adopt an ultrasonic extraction method to extract pesticide residues in textiles. In the paper published in the 2 nd of volume 36 of 2019 of printing and dyeing auxiliary agent of Li Shi just and the like, the method for extracting formaldehyde by ultrasonic is adopted during the extraction of a textile sample, and the pretreatment is simpler. The article published by dang et al in dyeing and finishing technology, 2017, volume 39, No. 5, "textile pH non-destructive detection based on ultrasonic extraction technology" developed a non-destructive detection method of textile pH by means of ultrasonic extraction technology. Compared with the conventional extraction method, the ultrasonic extraction method has the advantages of short extraction time, high efficiency, no need of heating and the like.

Microfluidic chip technology is a scientific technology mainly characterized by the manipulation and control of fluid and biological particles or cells in the fluid in a micrometer scale space, and is also called Lab-on-a-chip (LOC) or micro total analysis system (μ TAS). An ultrasonic microfluidics (acoustoflucis) chip is a microfluidic chip which utilizes ultrasonic waves as an external force field and can be called as a branch of the microfluidic chip. The realization of the integration of ultrasonic functions on a single microfluidic chip is always one of the important development directions of microfluidic technology.

Therefore, how to provide a textile detection chip applying ultrasonic extraction and microfluidic extraction technologies is a problem that needs to be solved urgently by those skilled in the art.

Disclosure of Invention

In view of the above, the invention provides a microfluidic chip integrating ultrasonic extraction and detection of textiles, which applies an ultrasonic extraction technology to the pretreatment of textiles and integrates the ultrasonic extraction technology and the textile detection technology on one chip, so that the detection of the textiles is greatly facilitated, and the microfluidic chip has the advantages of high efficiency, low consumption, high flux and the like, provides an effective technical means for determination and evaluation of the quality safety of the textiles, and is an important development direction of textile detection.

In order to achieve the purpose, the invention adopts the following technical scheme:

a micro-fluidic chip for integrated textile ultrasonic extraction and detection comprises a channel layer and a cover plate layer;

the channel layer comprises a chip substrate and at least one sample detection unit; the sample detection unit is positioned on the chip substrate; the sample detection unit includes: an ultrasonic extraction pool, a liquid separation pool, a detection pool and a microfluidic channel; the ultrasonic extraction pool is communicated with the liquid separation pool through a microfluidic channel, and the liquid separation pool is communicated with the detection pool through the microfluidic channel;

the cover plate layer is located above the channel layer, and an opening is formed in the cover plate layer and used for putting a sample to be tested into the ultrasonic extraction pool through the opening.

Preferably, the cover plate layer comprises an extraction pool cover matched with the opening, and the extraction pool cover is used for providing a channel inlet for placing a sample to be detected and an extracting solution into the ultrasonic extraction pool; and the extraction tank cover is provided with an extraction tank cover hole, and the extraction tank cover hole is used for gas-liquid circulation when the microfluidic chip is centrifuged.

Preferably, the bottom of the ultrasonic extraction pool is contacted with an ultrasonic generator.

Preferably, divide the liquid pond to set up one at least, and with detect pond quantity and correspond, it has textile quality safety inspection reagent to detect in advance in the pond, detect pond regional material and be transparent material.

Preferably, the centrifugal detection device further comprises a central fixing hole, wherein the central fixing hole is positioned at the geometric center of the channel layer and the cover plate layer and penetrates through the channel layer and the cover plate layer in an overlapped mode, and is used for being fixedly installed on the centrifugal detection device.

Preferably, the reagent pre-stored in the detection cell comprises a formaldehyde detection reagent or a pH color development reagent; the formaldehyde detection reagent is an alkaline phloroglucinol reagent; the pH detection reagent comprises one or two of bromocresol green, methyl red, methyl orange, indigo disulfonic acid sodium, bromophenol blue, alizarin sodium sulfonate, catechol violet, phenolsulfonphthalein, cresol red, bromothymol blue, m-cresol violet and naphthol benzyl alcohol.

Preferably, the detection steps of the microfluidic chip are as follows:

the method comprises the following steps: placing the textile in an ultrasonic extraction tank, adding an extracting solution, installing a microfluidic chip on centrifugal detection equipment, and setting a program by a control unit;

step two: ultrasonically extracting a textile extracting solution, adding the textile extracting solution into a sample detection pool through a centrifugal force or a sample injection unit, and reacting the textile extracting solution with a detection reagent prestored in the detection pool to obtain a reaction product;

step three: the detection pool is matched with the photoelectric detection mechanism, the collected information is sent to the control unit, and the control unit analyzes the information to obtain the content of the factors to be detected of the textile.

Preferably, in the second step, when the microfluidic chip is a centrifugal chip, the microfluidic chip is mounted on a centrifugal device through a central fixing hole, and the centrifugal device is moved to drive the microfluidic chip to rotate, so that the textile extract in the ultrasonic extraction pool flows into the detection pool, and a corresponding reaction product is generated.

Through the technical scheme, compared with the prior art, the invention has the beneficial effects that:

the textile sample pretreatment adopts an ultrasonic extraction technology, and adds textile sample extracting solution into a microfluidic chip through centrifugal equipment or a control sample injection pump. In addition, the detection reagent is fixed in the specific detection area of the microfluidic chip in advance, the detection reagent does not need to be configured during operation, the operation process is simplified, no professional is needed, and the detection kit can be used with matched portable equipment to realize on-site rapid detection. According to the invention, the ultrasonic pretreatment and detection of the textile are integrated into the same chip, so that the complex operation and manual error are avoided, the quality safety detection of the textile is more convenient, and the accuracy and timeliness of the quality safety detection of the textile are greatly improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts;

fig. 1 is a top view of a microfluidic chip for integrated textile ultrasonic extraction and detection provided in an embodiment of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The micro-fluidic chip for integrated textile ultrasonic extraction and detection disclosed by the embodiment applies an ultrasonic technology to textile pretreatment, integrates the ultrasonic technology and the detection into a system, extracts a factor to be detected more efficiently, avoids complex operation and artificial errors, makes textile quality safety detection more convenient, and greatly improves the accuracy and the timeliness of textile quality safety detection.

The microfluidic chip for integrated textile ultrasonic extraction and detection provided by the embodiment comprises a channel layer and a cover plate layer;

the channel layer comprises a chip substrate 1 and at least one sample detection unit; the sample detection unit is positioned on the chip substrate 1; the sample detection unit includes: an ultrasonic extraction pool 2, a liquid separation pool 4, a detection pool 5 and a microfluidic channel 3; wherein, the ultrasonic extraction pool 2 is communicated with the liquid separation pool 4 through the microfluidic channel 3, and the liquid separation pool 4 is communicated with the detection pool 5 through the microfluidic channel 3;

the apron layer is located the channel layer top, is provided with the opening on the apron layer for put in the sample that awaits measuring to ultrasonic extraction pond 2 through the opening.

In one embodiment, the cover plate layer comprises an extraction cell cover matched with the opening, and the extraction cell cover is used for providing a channel inlet for putting a sample to be tested and an extracting solution into the ultrasonic extraction cell 2; the extraction tank cover is provided with an extraction tank cover hole which is used for gas-liquid circulation when the microfluidic chip is centrifuged.

In one embodiment, the bottom of the ultrasonic extraction cell 2 is in contact with an ultrasonic generator.

In one embodiment, at least one liquid separation tank 4 is arranged, the number of the liquid separation tanks corresponds to that of the detection tanks 5, textile quality safety detection reagents are pre-stored in the detection tanks 5, and the material of the detection tanks 5 is transparent.

In one embodiment, the device further comprises a central fixing hole 6, wherein the central fixing hole 6 is positioned at the geometric center of the channel layer and the cover plate layer and is overlapped to penetrate through the channel layer and the cover plate layer for being fixedly installed on the centrifugal detection device.

In one embodiment, the reagent pre-stored in the detection cell 5 comprises a formaldehyde detection reagent or a pH chromogenic reagent; the formaldehyde detection reagent is an alkaline phloroglucinol reagent; the pH detection reagent comprises one or two of bromocresol green, methyl red, methyl orange, indigo disulfonic acid sodium, bromophenol blue, alizarin sodium sulfonate, catechol violet, phenolsulfonphthalein, cresol red, bromothymol blue, m-cresol violet and naphthol benzyl alcohol.

In one embodiment, the detection steps of the microfluidic chip are as follows:

the method comprises the following steps: placing the textile in an ultrasonic extraction tank 2, adding an extracting solution, installing a microfluidic chip on centrifugal detection equipment, and setting a program by a control unit;

step two: ultrasonically extracting a textile extracting solution, adding the textile extracting solution into a sample detection pool 5 through a centrifugal force or a sample injection unit, and reacting the textile extracting solution with a detection reagent prestored in the detection pool 5 to obtain a reaction product;

step three: the detection pool 5 is matched with the photoelectric detection mechanism, the collected information is sent to the control unit, and the control unit analyzes the information to obtain the content of the factors to be detected of the textile.

In one embodiment, in the second step, when the microfluidic chip is a centrifugal chip, the microfluidic chip is mounted on the centrifugal device through the central fixing hole 6, and the microfluidic chip is driven to rotate when the centrifugal device moves, so that the textile extract in the ultrasonic extraction cell 2 flows into the detection cell 5, and a corresponding reaction product is generated.

In one embodiment, the microfluidic channel 3 is further communicated with a vent hole 8, and the vent hole 8 corresponds to the extraction cell cover hole.

In one embodiment, the cover plate 9 of the extraction tank is provided with an application hole 7 for applying a sample or an extraction solution to be tested to the ultrasonic extraction tank 2 directly through the application hole 7.

The micro-fluidic chip for integrated textile ultrasonic extraction and detection provided by the invention is described in detail, a specific example is applied in the description to explain the principle and the implementation mode of the invention, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.