阅读说明：本技术 一种含有桧木醇和植物精油及其主要成分的杀菌组合物在防治植物病害和霉菌中的用途 (Application of bactericidal composition containing hinokitiol and plant essential oil and main components thereof in preventing and treating plant diseases and mold ) 是由 刘映前 周锐 张智军 王朝辉 贺颖慧 杜莎莎 吴天琳 赵文斌 杨程杰 唐晨 胡勇 于 2021-01-19 设计创作，主要内容包括：本发明公开了一种含有桧木醇和植物精油及其主要成分的杀菌组合物,有效成分为桧木醇和牛至精油,丁香精油,百里香精油,香芹酚,丁香酚和百里酚。桧木醇和牛至精油,丁香精油,百里香精油,香芹酚,丁香酚和百里酚的质量份数比为1:1,1:10,1:30,1:50。本发明杀菌组合物可用于防治立枯丝核菌、油菜菌核病、番茄灰霉病、小麦赤霉病、稻瘟病、棉花枯萎病和黄曲霉。本发明组分均为天然产物,对环境无害。可望减少农药对环境的影响,降低用药成本,能延缓或克服病害抗药性的产生,延长药剂的使用寿命,有利于病害的综合管理。(The invention discloses a bactericidal composition containing hinokitiol and plant essential oil and main components thereof. The weight portion ratio of the hinokitiol to the origanum vulgare essential oil to the hinoki essential oil is 1:1,1:10, 1:30 and 1: 50. The bactericidal composition can be used for preventing and treating rhizoctonia solani, sclerotinia rot of colza, gray mold of tomato, scab of wheat, rice blast, fusarium wilt of cotton and aspergillus flavus. The components of the invention are all natural products and are harmless to the environment. The pesticide composition can reduce the influence of pesticide on the environment, reduce the pesticide cost, delay or overcome the generation of drug resistance of diseases, prolong the service life of the pesticide composition and be beneficial to the comprehensive management of the diseases.)

1. A bactericide composition containing hinokitiol and plant essential oil and main components thereof is characterized in that: the effective components include hinokitiol and origanum oil, clove oil, thyme oil, carvacrol, eugenol and thymol. The weight portion ratio of the hinokitiol to the origanum vulgare essential oil to the hinoki essential oil is 1:1,1:10, 1:30 and 1: 50.

2. The composition of claim 1 having the following molecular structural features:

3. the use of the binary composition of claim 1, wherein the binary composition comprises hinokitiol, oregano essential oil, clove essential oil, thyme essential oil, carvacrol, eugenol, and thymol, for controlling a disease caused by rhizoctonia solani.

4. The use of a binary composition comprising hinokitiol, oregano essential oil, clove essential oil, thyme essential oil, carvacrol, eugenol, thymol according to claim 1 for the control of sclerotinia rot of colza.

5. The use of the binary composition of claim 1, wherein the binary composition comprises hinokitiol, essential oil of origanum vulgaris, essential oil of clove, essential oil of thyme, carvacrol, eugenol, and thymol, for preventing and treating tomato gray mold.

6. The use of the binary composition of claim 1, wherein the binary composition comprises hinokitiol, oregano essential oil, clove essential oil, thyme essential oil, carvacrol, eugenol, and thymol, for preventing and treating wheat scab.

7. The use of the binary composition of claim 1, wherein the binary composition comprises hinokitiol, oregano essential oil, clove essential oil, thyme essential oil, carvacrol, eugenol, and thymol.

8. The use of the binary composition comprising hinokitiol, oregano essential oil, clove essential oil, thyme essential oil, carvacrol, eugenol, thymol according to claim 1 for preventing and treating cotton wilt.

9. The use of the binary composition comprising hinokitiol, oregano essential oil, clove essential oil, thyme essential oil, carvacrol, eugenol, thymol according to claim 1 for controlling aspergillus flavus.

Technical Field

The invention relates to a bactericidal composition which takes junenol and plant essential oil and main components thereof as effective components and is used for preventing and treating plant diseases and aspergillus flavus caused by rhizoctonia solani, sclerotinia sclerotiorum, tomato gray mold, wheat scab, rice blast and cotton fusarium wilt.

Background

The plant pathogenic fungi are one of the most common and most harmful diseases in the crop cultivation process, and the annual loss of the crop and cotton production all over the world is more than 10 percent according to the estimation of the grain and crop organization of the United nations. Wheat and rice are used as main grain crops in China, rape, tomatoes, cotton, potatoes, corn, watermelons, hot peppers and the like are used as main economic crops in China, diseases of different degrees exist at present, the reduction of crop yield can be caused, and then huge economic loss is caused, and the quality safety and international trade of agricultural products are seriously threatened to a certain extent. Grain storage is an effective means of avoiding grain shortage. The aspergillus flavus is easy to breed in the grain food, and great hidden trouble is brought to the grain safety. At present, chemical control is still the mainstream control means at present among a plurality of measures for controlling plant pathogenic fungi, and high-efficiency control effect can be generally obtained at the early stage of control. However, due to long-term unreasonable use, fungi generate resistance of different degrees to the chemical bactericides, so that the ideal bactericidal effect can be achieved only by increasing the dosage, and further, the medicine has more residues and strong toxic and side effects. In order to cope with the drug resistance, the dosage of the chemical bactericide is reduced, and the drug combination is particularly important, so that the dosage of the drug can be reduced, the sensitivity of germs to the bactericide is increased, the antibacterial spectrum is expanded, the environmental pollution is reduced, and more importantly, the occurrence of the drug resistance of the bactericide can be slowed down.

The hinokitiol is a monoterpene natural compound extracted from Chinese arborvitae, has antibacterial, antioxidant and antitumor effects, and is a high-safety plant secondary metabolite. Research shows that the hinokitiol can control postharvest diseases of peach and eggplant and maintain postharvest quality.

Plant Essential Oil (EO) is a generic term for fragrant (and/or scented) volatile oily liquid substances extracted from plant tissues such as roots, stems, leaves, flowers, fruits, seeds, branches or secretions. The main components are terpenes, phenols, aldehydes, alcohols, esters and other organic substances. Research in recent decades shows that essential oils and single components have strong properties of inhibiting or killing microorganisms such as bacteria and fungi.

The experiment shows that the combination application of the juniperol and the plant essential oil and the main components thereof has obvious synergistic effect on preventing and treating rhizoctonia solani, sclerotinia rot of colza, gray mold of tomato, scab of wheat, rice blast, fusarium wilt of cotton and aspergillus flavus. The control effect is improved, the dosage can be reduced, and the requirement of 'zero increase of pesticide' is met.

Disclosure of Invention

The invention aims to provide a bactericide composition containing junenol and plant essential oil and main components thereof, which is used for preventing and treating rhizoctonia solani, sclerotinia rot of colza, gray mold of tomato, scab of wheat, rice blast, fusarium wilt of cotton and aspergillus flavus.

In order to achieve the purpose, the technical scheme of the invention is as follows:

a bactericide composition containing hinokitiol and plant essential oil and main components thereof comprises an active component A and an active component B, wherein the active component A is hinokitiol, the active component B is plant essential oil and main components thereof, and the weight part ratio of the active component A to the active component B is 1:1,1:10, 1:30 and 1: 50.

The bactericide composition provided by the invention has the following beneficial effects:

1) the bactericidal composition provided by the invention shows obvious synergistic action in a certain proportioning range, and the control effect of the composition is obviously improved compared with that of a single agent.

2) The bactericidal composition provided by the invention improves the prevention and treatment effect, reduces the medication cost and expands the antibacterial spectrum.

3) The bactericidal composition provided by the invention is a safe and green natural product, is harmless to the environment, and meets the requirements of green agriculture and green development.

4) The effective components of the bactericidal composition provided by the invention have different action mechanisms, and the application of the composition can delay or overcome the generation of drug resistance of diseases, prolong the service life of the medicament and be beneficial to the comprehensive treatment of the diseases.

Detailed Description

In order to better understand the essence of the present invention, the following examples are further illustrated, but the present invention is not limited to these examples. In these examples, all percentages are by weight unless otherwise indicated.

Example 1: a bactericide composition containing hinokitiol and plant essential oil and its main components comprises active component A and active component B (shown as formula 1), wherein the active component A is hinokitiol, and the active component B is plant essential oil and its main component.

Example 2: indoor activity determination of hinokitiol and active component B mixed with antitrichosis

The plant pathogenic bacteria used in the experiment are strains preserved at 4 ℃ in a laboratory, and the adopted culture medium is a potato agar glucose culture medium (PDA for short). The PDA culture medium formula comprises: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL, natural pH.

The compounding synergy of the bactericide is calculated according to the formula of Wadley (1967) to obtain the synergistic coefficient after mixing different medicaments:

in the formula, a and B respectively represent the ratio of two medicaments of sabinol (A), vegetable essential oil and main component (B), EC₅₀(A)，EC₅₀(B) Respectively represent actual observed EC₅₀Value, EC₅₀(Exp) represents the theoretical EC for the mixture of A and B agents in a: B ratio₅₀Value, EC₅₀(Obs) actual observed EC after mixing of A and B agents₅₀The value is obtained. SR>1.5 is synergy; SR<0.5 is antagonistic; an SR between 0.5 and 1.5 indicates additive effect.

The indoor toxicity synergy of the combination of hinokitiol and the active component B against Rhizoctonia solani is shown in Table 1.

TABLE 1 indoor toxicity synergism of blending of sabinenol and active ingredient B against Rhizoctonia solani

As can be seen from Table 1, when the weight ratio of the sabinenol to the active component B is 1:1,1:10, 1:30 and 1:50, the synergistic effect on sclerotinia rot of rape is obvious, and especially when the weight ratio of the sabinenol to the butyl essential oil is 1:50, the SR is 2.13.

Example 2: indoor activity determination of sabinol and active component B mixed to sclerotinia rot of rape

The indoor toxicity synergism of the combination of hinokitiol and the active component B on the sclerotinia rot of colza is shown in Table 2.

TABLE 2 indoor toxicity synergism of blending of sabinenol and active ingredient B on Sclerotinia sclerotiorum

As can be seen from Table 2, when the weight ratio of the sabinenol to the active component B is 1:1,1:10, 1:30 and 1:50, the synergistic effect on sclerotinia sclerotiorum is achieved, particularly, when the sabinenol and the plant essential oil are compounded, the synergistic effect is obvious, and when the weight ratio is 1:1, the SR values of the sabinenol and the three plant essential oils are respectively as high as 41.69, 27.28 and 14.81.

Example 3: indoor activity determination of tomato gray mold by mixing hinokitiol and active component B

The indoor toxicity synergism of the combination of hinokitiol and the active component B on the tomato gray mold is shown in Table 3.

TABLE 3 indoor toxicity synergism of Sabina chinensis alcohol and active component B blending on tomato Botrytis cinerea

As can be seen from Table 3, when the mixing mass ratio of the sabinenol to the active component B is 1:1,1:10, 1:30 and 1:50, the synergistic effect on the tomato gray mold is basically avoided, and the composition is in an additive effect. Only in hinokitiol: the clove essential oil shows a synergistic effect when the ratio of the clove essential oil to the clove essential oil is 1:50, and the SR is 1.65.

Example 4: indoor activity determination of wheat scab by mixing hinokitiol and active component B

The indoor toxicity synergism of the combination of hinokitiol and the active component B on wheat scab is shown in Table 4.

TABLE 4 indoor toxicity synergism of blending of sabinenol and active ingredient B on wheat scab

As can be seen from Table 4, when the mixing mass ratio of sabinol to active component B is 1:1,1:10, 1:30 and 1:50, no synergistic effect is caused on wheat scab, and all the compositions are in additive effect.

Example 5: indoor activity determination of rice blast by mixing hinokitiol and active component B

The indoor toxicity synergism of the combination of hinokitiol and active component B on rice blast is shown in Table 5.

TABLE 5 indoor toxicity synergism of blending of sabinenol and active ingredient B on Rice blast

As can be seen from Table 5, the synergistic effect on rice blast is only achieved at 1:1 when the weight ratio of sabinol to active component B is 1:1,1:10, 1:30 and 1:50, and all the other compositions are in additive effect.

Example 6: indoor activity determination of sabinol and active component B mixed on cotton wilt pathogen

The indoor toxicity synergism of the combination of hinokitiol and the active component B on cotton wilt pathogens is shown in Table 6.

TABLE 6 indoor toxicity synergism of blending of sabinenol and active ingredient B on cotton wilt pathogen

As can be seen from Table 6, when the mixing mass ratio of sabinol to active component B is 1:1,1:10, 1:30 and 1:50, no synergistic effect is caused on cotton wilt, and all the compositions are in additive effect.

Example 7: aspergillus flavus indoor activity determination by mixing hinokitiol and active component B

The indoor toxicity synergism of the combination of hinokitiol and the active component B on Aspergillus flavus is shown in Table 7.

TABLE 7 indoor toxicity synergism of Juniperol and active component B on Aspergillus flavus

As can be seen from Table 7, when the mixing mass ratio of the hinokitiol to the active component B is 1:1,1:10, 1:30 and 1:50, the synergistic effect on the aspergillus flavus is obvious, particularly when the mixing mass ratio is three, namely 1:1,1:10 and 1:50, the SR is at most 4.35, and the rest compositions are in additive effect.

In summary, it can be seen that the antibacterial activity of the combination of hinokitiol and plant essential oil and the main components thereof is higher than that of a single agent at the same concentration, the combination is particularly prominent in preventing and treating sclerotinia sclerotiorum, pyricularia oryzae and aspergillus flavus, and shows obvious synergistic effect, such as hinokitiol: origanum essential oil is 1:1, and when the origanum essential oil is used for resisting sclerotinia sclerotiorum, the SR is as high as 41.69; hinokitiol: 1:1 of clove essential oil, wherein the SR is 4.54 when the clove essential oil resists rice blast germs; hinokitiol: carvacrol is 1:10, and when the carvacrol is resistant to rice blast germs, the SR is 3.45; all compositions showed synergistic and additive effects with no antagonism. The invention is beneficial to prolonging the service life of the medicament and comprehensively treating diseases.