阅读说明：本技术 一种蜜蜂趋避剂及其制备方法和应用 (Bee repellent and preparation method and application thereof ) 是由 文平 于 2021-01-19 设计创作，主要内容包括：本发明公开了一种蜜蜂趋避剂及其制备方法和应用,该趋避剂由包括如下重量份原料制得：乙酸苄酯0～1份、(E)-癸-2-烯基乙酸酯0～1份、丙位辛内酯0～1份和(E)-辛-2-烯基乙酸酯0～1份；所述乙酸苄酯、(E)-癸-2-烯基乙酸酯、丙位辛内酯和(E)-辛-2-烯基乙酸酯不同时为0份；本发明的蜜蜂驱避剂可高效的驱避蜜蜂的采集,田间和林间施用时可高效驱避蜜蜂不访问施用区域,并通过与杀虫剂联合施用时,可有效减少蜜蜂对农药施用区的访问,减少蜜蜂对杀虫剂的暴露接触,避免杀虫剂杀害蜜蜂。(The invention discloses a bee repellent and a preparation method and application thereof, wherein the repellent is prepared from the following raw materials in parts by weight: 0-1 part of benzyl acetate, 0-1 part of (E) -decyl-2-alkenyl acetate, 0-1 part of propiolactone and 0-1 part of (E) -octyl-2-alkenyl acetate; the benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate are not 0 part at the same time; the bee repellent can efficiently repel the bees for collection, can efficiently repel the bees without visiting the application area when being applied in the field and in the forest, can effectively reduce the visit of the bees to the pesticide application area, reduce the exposure contact of the bees to the pesticide and avoid the pesticide from killing the bees when being applied together with the pesticide.)

1. The bee repellent is characterized by being prepared from the following raw materials in parts by weight:

0-1 part of benzyl acetate, 0-1 part of (E) -decyl-2-alkenyl acetate, 0-1 part of propiolactone and 0-1 part of (E) -octyl-2-alkenyl acetate;

and the benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate are not 0 part at the same time.

2. The bee repellent according to claim 1, which is prepared from the following raw materials in parts by weight:

benzyl acetate 1 part, decyl-2-alkenyl acetate 0.2 parts, propyl octyl lactone 0.2 parts, and octyl-2-alkenyl acetate 0.2 parts.

3. The bee repellent according to claim 1 or 2, wherein said raw material further comprises a slow release carrier, and the amount of said slow release carrier is 0.25-1 times of the total weight of benzyl acetate, (E) -dec-2-enyl acetate, propiolactone and (E) -oct-2-enyl acetate.

4. The bee repellent according to claim 3, wherein said slow release carrier is an oil-in-water emulsifier.

5. The bee repellent according to claim 4, wherein said oil-in-water emulsifier is prepared by mixing and stirring non-ionic liquid paraffin and microemulsion NMP at a ratio of 1: 1.

6. A method of preparing a bee repellent according to any of claims 1 to 5, comprising:

and (2) uniformly mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain a mixture, adding a slow release carrier into the mixture, and uniformly stirring to obtain the bee repellent.

7. A method for avoiding bees in a field, which comprises applying the bee repellent according to any one of claims 1 to 5 or the bee repellent prepared by the preparation method according to claim 6 to the field.

8. The bee evasion method of claim 7, wherein said evasion agent is applied in an amount of 1-10 g per acre of said field.

9. A bee-killing pesticide, which is characterized by comprising the bee repellent according to any one of claims 1 to 5 or the bee repellent prepared by the preparation method according to claim 6, and a spraying pesticide.

10. The bee-killing-free pesticide according to claim 9, wherein the spraying pesticide is at least one selected from the group consisting of 20% tebufenozide water emulsion, 10% fipronil emulsion, 10% imidacloprid emulsion, 10% aphicide emulsion, 10% chlorpyrifos emulsion and 10% cyhalothrin water emulsion.

Technical Field

The invention relates to the technical field of bee avoidance, in particular to a bee avoidance agent and a preparation method and application thereof.

Background

Bees are pollinating insects for a wide variety of plants, including most fruits and crops. Bee pollination directly affects the yield and quality of most agricultural products. With the development of modern agriculture, people rely heavily on pesticides to control pests in agriculture and forestry, which causes serious damage to the health of bees. In some areas the crisis arises where the bee population density decreases sharply. This crisis is also gaining attention globally. However, the agroecological system is extremely fragile and many agricultural production activities cannot be protected from pesticides. Although people have tried to use pesticides that are environmentally friendly to humans, since bees are by nature insect attributes, there is no effective way to reduce the damage to bees. Such as bee colony collapse syndrome (CCD) caused by pesticides such as imidacloprid which are recently appeared in the world. In order to find a compromise, it is desirable to repel bees during the insecticide application period to reduce direct contact of bees with the insecticide and to preserve the health of the bee population in the field to some extent.

The alarm pheromone of the bee has a behavior ecological function of informing the bee of the feeding risk. This has attracted the attention of researchers. Some researchers have reported that bees are repelled by using components with higher volatility in bee siren, such as Isopentyl acetate, 2-heptanone, etc. But the components have the characteristic of overhigh volatility, thereby generating the defects of overhigh consumption and extremely short effect duration and having no practical field use value.

Disclosure of Invention

The invention aims to provide a bee repellent, a preparation method and application thereof, the repellent can effectively interfere the collection behavior of bees on crops applying pesticides, reduces the exposure contact of the bees to the pesticides, has long duration, can be compounded with the pesticides, and effectively avoids the pests of the pesticides to the bees.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

the invention provides a bee repellent, which is prepared from the following raw materials in parts by weight:

0-1 part of benzyl acetate, 0-1 part of (E) -decyl-2-alkenyl acetate, 0-1 part of propiolactone and 0-1 part of (E) -octyl-2-alkenyl acetate;

and the benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate are not 0 part at the same time.

Preferably, the mass ratio of the benzyl acetate, (E) -deca-2-alkenyl acetate, the propiolactone, and the (E) -oct-2-alkenyl acetate is 1: 0.25: 0.5: 0.25;

preferably, the bee repellent is prepared from the following raw materials in parts by weight:

benzyl acetate 1 part, decyl-2-alkenyl acetate 0.2 parts, propyl octyl lactone 0.2 parts, and octyl-2-alkenyl acetate 0.2 parts.

Preferably, the raw materials further comprise a slow release carrier, and the addition amount of the slow release carrier is 0.25-1 time of the total weight of benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octyl lactone and (E) -octyl-2-alkenyl acetate. The slow release rate is 0.1-1 mug/min; by adding the slow release carrier, the lasting time of the effective components for repelling bees can be effectively prolonged, and the protection effect of the pesticide on the bees during the pesticide application period is increased.

Preferably, the sustained release carrier is an oil-in-water emulsifier or a sustained release capsule; more preferably, the oil-in-water emulsifier is prepared by mixing, stirring and homogenizing nonionic liquid paraffin and a microemulsion NMP according to a ratio of 1:1, and the sustained-release capsule is prepared by high-speed shearing starch sodium octenyl succinate, emulsified modified starch, maltodextrin and beta-cyclodextrin according to a ratio of 15:5:3: 2.

The second aspect of the present invention provides a preparation method of the bee repellent, wherein the preparation method comprises:

and (2) uniformly mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain a mixture, adding a slow release carrier into the mixture, and uniformly stirring to obtain the bee repellent.

The third aspect of the invention provides a method for avoiding bees in a field forest, which comprises applying the bee repellent or the bee repellent prepared by the preparation method to the field forest.

Preferably, the amount of the repellent applied to each acre of the field is 1-10 g.

The fourth aspect of the invention provides a pesticide for avoiding killing bees, which comprises the bee repellent or the bee repellent prepared by the preparation method and a spraying pesticide.

Preferably, the volume ratio of the bee repellent to the spraying insecticide is (2-5): 20.

preferably, the spraying insecticide is at least one selected from 20% tebufenozide aqueous emulsion, 10% fipronil emulsion, 10% imidacloprid emulsion, 10% triazophos emulsion, 10% chlorpyrifos emulsion and 10% cyhalothrin aqueous emulsion.

Compared with the prior art, the invention has the beneficial effects that at least:

the inventor of the invention discovers through a large amount of experimental researches that: the honeybee can efficiently intercept and utilize alarm signals of other species, so that the repellent based on the honeybee instinct alarm behavior has no species specificity; the honeybee repellent can efficiently repel the honeybees to collect the honeybees, and can efficiently repel the honeybees to avoid visiting an application area when the honeybee repellent is applied to fields and forests.

When the honeybee repellent and the insecticide are jointly applied, the visit of honeybees to an insecticide application area can be effectively reduced, the exposure contact of the honeybees to the insecticide is reduced, and the honeybees are prevented from being killed by the insecticide.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Detailed Description

The following describes embodiments of the present invention in detail with reference to the following embodiments. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.

The slow release carrier oil-in-water emulsifier adopted in the following examples is prepared by mixing, stirring and homogenizing non-ionic liquid paraffin and a microemulsion NMP according to a ratio of 1: 1.

Example 1

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate, 1 part of (E) -decyl-2-alkenyl acetate, 1 part of propyl-octalactone and 1 part of (E) -octa-2-alkenyl acetate; and 1 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain mixture, adding slow release carrier into the mixture, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 10% cyhalothrin aqueous emulsion as pesticide;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 2

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate, 1 part of (E) -decyl-2-alkenyl acetate, 1 part of propyl-octalactone and 1 part of (E) -octa-2-alkenyl acetate; and 1 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain mixture, adding slow release carrier into the mixture, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying insecticide into 10% imidacloprid emulsion;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 3

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate, 1 part of (E) -decyl-2-alkenyl acetate, 1 part of propyl-octalactone and 1 part of (E) -octa-2-alkenyl acetate; and 1 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain mixture, adding slow release carrier into the mixture, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 10% chlorpyrifos emulsion as pesticide;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 4

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate, 1 part of (E) -decyl-2-alkenyl acetate, 1 part of propyl-octalactone and 1 part of (E) -octa-2-alkenyl acetate; and 1 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain mixture, adding slow release carrier into the mixture, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 10% of aphicide-killing phosphorus emulsion;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 5

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate, 1 part of (E) -decyl-2-alkenyl acetate, 1 part of propyl-octalactone and 1 part of (E) -octa-2-alkenyl acetate; and 1 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain mixture, adding slow release carrier into the mixture, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 20% tebufenozide aqueous emulsion;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 6

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate, 1 part of (E) -decyl-2-alkenyl acetate, 1 part of propyl-octalactone and 1 part of (E) -octa-2-alkenyl acetate; and 1 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain mixture, adding slow release carrier into the mixture, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 10% fipronil emulsion as pesticide;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 7

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate; and 0.25 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

adding the slow release carrier into benzyl acetate, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying insecticide into 10% imidacloprid emulsion;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 8

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of gamma-octanolide; and 0.25 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

adding the slow release carrier into the gamma-octanoic lactone and uniformly stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 10% fipronil emulsion as pesticide;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 9

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

(E) -dec-2-enyl acetate and 1 part and (E) -oct-2-enyl acetate 1 part; and 0.5 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

uniformly mixing (E) -deca-2-alkenyl acetate and (E) -octyl-2-alkenyl acetate to obtain a mixture, adding a slow release carrier into the mixture, and uniformly stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying insecticide into 10% imidacloprid emulsion;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 10

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

(E) -deca-2-enyl acetate and 1 part and benzyl acetate 1 part; and 0.5 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

uniformly mixing (E) -deca-2-alkenyl acetate and benzyl acetate to obtain a mixture, adding a slow-release carrier into the mixture, and uniformly stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 10% fipronil emulsion as pesticide;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 11

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate, 0.25 part of (E) -decyl-2-alkenyl acetate, 0.5 part of propyl-octalactone and 0.25 part of (E) -octa-2-alkenyl acetate; and 0.5 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain mixture, adding slow release carrier into the mixture, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 10% cyhalothrin aqueous emulsion as pesticide;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Example 12

First, bee repellent

The bee repellent is prepared from the following raw materials in parts by weight:

1 part of benzyl acetate, 0.2 part of (E) -decyl-2-alkenyl acetate, 0.2 part of propyl octalactone and 0.2 part of (E) -octyl-2-alkenyl acetate; and 0.4 part of slow release carrier;

second, preparation method

The preparation method of the bee repellent comprises the following steps:

mixing benzyl acetate, (E) -decyl-2-alkenyl acetate, propyl octalactone and (E) -octyl-2-alkenyl acetate to obtain mixture, adding slow release carrier into the mixture, and stirring to obtain the bee repellent;

insecticide for killing bees

The insecticide for avoiding killing the bees comprises the bee repellent and a spraying insecticide, wherein the volume ratio of the bee repellent to the spraying insecticide is 5: 20;

spraying 10% cyhalothrin aqueous emulsion as pesticide;

method for avoiding bees in field

The field bee avoidance method comprises the following steps: diluting 25ml of the bee-killing-free pesticide by 5000 times, and spraying the diluted pesticide to 1 mu of rape field in flowering phase.

Examples of the experiments

The test is respectively carried out aiming at the eastern bees and the western bees, and the specific test method comprises the following steps:

the field bee avoidance methods in the above examples 1 to 12 were respectively adopted for the experiments, and the experimental methods for the eastern bees and the western bees were respectively as follows:

the oriental bees and the western bees are treated the same, and in each embodiment, the number of dead bees at the door opening of the Wangqun bee hive, the number of individuals of which is more than 5000 at 50 meters beside the area to be applied with the medicine is continuously counted for 5 days, which is the purchased test hive; after the honeybee repellent insecticide is applied to crops, the number of dead honeybees beside the application area is continuously observed within 5 days; directly treating one mu of land on the other side with pesticide, and continuously observing the death number of bees within 5 days; the effect of repelling on bee protection is verified by analyzing and comparing the change of the number of deaths before and after the administration with the dosage form of whether to repel the bees;

the statistical results are shown in table 1:

table 1 repellent compositions and technical effect tabulation of each example

As can be seen from the data in Table 1:

the repellent can effectively repel most of collected bees so as to reduce the activity of bees in areas and further reduce the contact of the bees to the insecticide, thereby protecting the bees under the condition of using the insecticide.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.