阅读说明：本技术 一种电流刺激装置及电流刺激成骨细胞分化治疗系统 (Current stimulation device and current stimulation osteoblast differentiation treatment system ) 是由 周晓中 张应子 李舟 胥玲玲 刘卓 于 2021-03-10 设计创作，主要内容包括：本发明公开了一种电流刺激装置及电流刺激成骨细胞分化治疗系统,涉及生物工程技术领域,包括纳米发电机,所述纳米发电机与刺激电极电连接,所述刺激电极与患处接触。本发明的刺激应用在骨折患者的患处,通过纳米发电机实现电流刺激,促进成骨细胞增殖,促进成骨细胞活性,本发明为骨折愈合提供了新的思路,促进了纳米发电机在可穿戴电子医疗器械中的应用进展。本发明具有便携性,具有良好的临床应用前景。(The invention discloses a current stimulation device and a current stimulation osteoblast differentiation treatment system, and relates to the technical field of bioengineering. The stimulation of the invention is applied to the affected part of the fracture patient, the current stimulation is realized through the nano generator, the osteoblast proliferation is promoted, and the osteoblast activity is promoted. The invention has portability and good clinical application prospect.)

1. An electrical current stimulation apparatus characterized by: the device comprises a nano generator, wherein the nano generator is electrically connected with a stimulation electrode, and the stimulation electrode is contacted with an affected part.

2. The current stimulation device of claim 1, wherein: the nano generator is arched.

3. The current stimulation device of claim 1, wherein: a rectifier bridge is arranged between the nano generator and the stimulating electrode.

4. The current stimulation device of claim 1, wherein: the nano generator is a piezoelectric nano generator and comprises a Kapton film, the Kapton film is formed by thermal molding, a first silver electrode and a second silver electrode are sequentially covered on the lower surface of the Kapton film, and the first silver electrode and the second silver electrode are electrically connected with the stimulating electrode through a lead.

5. The current stimulation device of claim 4, wherein: the first silver electrode is coated on the upper surface of a PVDF film, the second silver electrode is coated on the lower surface of the PVDF film, and the lower surface of the second silver electrode is coated with a PET film.

6. The current stimulation device of claim 5, wherein: the Kapton film, the first silver electrode, the PVDF film, the second silver electrode and the PET film are sequentially bonded through a silicone polymer.

7. The current stimulation device of claim 1, wherein: the nano generator is a friction nano generator.

8. The current stimulation device of claim 1, wherein: the stimulating electrode is a needle electrode.

9. The current stimulation device of claim 1, wherein: the stimulating electrode comprises a positive electrode and a negative electrode, the negative electrode is connected with the wound broken end of the affected part, and the positive electrode is connected with the muscle tissue adjacent to the wound broken end.

10. An electric current-stimulated osteoblast differentiation therapy system, comprising: comprising a current stimulation device according to any of the claims 1-9, wherein connection structures are arranged at both ends of the nanogenerator, and the connection structures are connected with clamping plates.

Technical Field

The invention relates to the technical field of bioengineering, in particular to a current stimulation device and a current stimulation osteoblast differentiation treatment system.

Background

The skeleton has the functions of movement, support and body protection, and has important significance for maintaining normal activities of human bodies. Bone fractures are a clinically common disease that results in complete or partial disruption of the continuity of bone structure. Mainly in children and the elderly. The healing process of the fracture lasts for a long time, is easily interfered by various factors to cause delay or even non-healing, and seriously influences the life quality of patients. Therefore, how to promote fracture repair and shorten the healing time is the leading edge and difficulty of orthopedic research.

With the intensive research on the fracture healing mechanism, various adjuvant treatment methods are widely applied clinically. For example, Bone Tissue Engineering (BTE) mimics autologous bone grafting in many ways, filling defective bone with scaffolds, osteoblasts, and modulating cell-cell and cell-scaffold interactions by the addition of growth factors or electrical stimulation. In particular, the physical therapy of electrical stimulation, the effect of which in promoting fracture healing has been well documented. The electric stimulation can not only promote the healing of fresh fracture, but also has good curative effects on delayed healing of fracture, nonunion, osteotomy, pseudoarthrosis and the like.

Electrical stimulation to promote osteogenesis is a key factor in bone remodeling repair. Can be divided into current stimulation, electric field stimulation and electromagnetic field stimulation. However, in clinical treatments, electrical stimulation devices are too bulky. A portable, patient-compliant electrical stimulation therapy device for bone repair remains a significant challenge.

Disclosure of Invention

The invention aims to provide a current stimulation device and a current stimulation osteoblast differentiation treatment system, which are used for solving the problems in the prior art, are beneficial to a bone repair process and have good flexibility and portability.

In order to achieve the purpose, the invention provides the following scheme:

the invention provides a current stimulation device which comprises a nano generator, wherein the nano generator is electrically connected with a stimulation electrode, and the stimulation electrode is contacted with an affected part.

Preferably, the nanogenerator has an arch shape.

Preferably, a rectifier bridge is arranged between the nano-generator and the stimulating electrode.

Preferably, the nano-generator is a piezoelectric nano-generator, the nano-generator comprises a Kapton film, the Kapton film is formed by thermal molding, a first silver electrode and a second silver electrode are sequentially coated on the lower surface of the Kapton film, and the first silver electrode and the second silver electrode are both electrically connected with the stimulation electrode through a lead.

Preferably, the first silver electrode is coated on the upper surface of a PVDF film, the second silver electrode is coated on the lower surface of the PVDF film, and the PET film is coated on the lower surface of the second silver electrode.

Preferably, the Kapton film, the first silver electrode, the PVDF film, the second silver electrode, and the PET film are sequentially bonded by a silicone polymer.

Preferably, the nano-generator is a tribo nano-generator.

Preferably, the stimulation electrode is a needle electrode.

Preferably, the stimulating electrode comprises a positive electrode and a negative electrode, the negative electrode is connected with the wound broken end of the affected part, and the positive electrode is connected with the adjacent muscle tissue of the wound broken end.

The invention also provides a current stimulation osteoblast differentiation treatment system, which comprises the current stimulation device, wherein two ends of the nano generator are provided with connecting structures, and the connecting structures are connected with the splint.

Compared with the prior art, the invention has the following technical effects:

the stimulation of the invention is applied to the affected part of the fracture patient, the current stimulation is realized through the nano generator, the osteoblast proliferation is promoted, and the osteoblast activity is promoted. The invention has portability and good clinical application prospect.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic view of an electrical current stimulation apparatus of the present invention;

FIG. 2 is a schematic diagram of a nanogenerator according to the invention;

FIG. 3 is a schematic diagram of the structure of each layer of the nano-generator of the present invention;

FIG. 4 is a schematic diagram of the application of the current-stimulated osteoblast differentiation therapy system of the present invention;

wherein: 100-current stimulation device, 200-current stimulation osteoblast differentiation treatment system, 1-nano generator, 2-rectifier bridge, 3-stimulation electrode, 4-Kapton membrane, 5-first silver electrode, 6-second silver electrode, 7-lead, 8-PVDF membrane, 9-PET membrane and 10-connection structure.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.

The invention aims to provide a current stimulation device and a current stimulation osteoblast differentiation treatment system, which are used for solving the problems in the prior art, are beneficial to a bone repair process and have good flexibility and portability.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

Example one

As shown in fig. 1-3: the present embodiment provides a current stimulation apparatus 100, which includes a nano-generator 1(sm-PENG) capable of converting biomechanical energy into electric energy, wherein the nano-generator 1 is electrically connected to a stimulation electrode 3, and the stimulation electrode 3 is in contact with an affected part. The output range of the nano generator 1 can be nA-mA level according to current, and the frequency can be 1 Hz-thousands Hz. The stimulation of this embodiment is applied to fracture patient's affected part, realizes the current stimulation through nanometer generator 1, promotes osteoblast proliferation, promotes osteoblast activity, and this embodiment provides new thinking for fracture healing, has promoted the application progress of nanometer generator 1 in wearable electronic medical instrument. The embodiment has portability and good clinical application prospect.

Specifically, in this embodiment, the nanogenerator 1 is arched, the arched structure greatly improves the electrical output performance of the nanogenerator 1, the short-circuit current can reach 20 μ a by tapping the nanogenerator 1, the short-circuit current is more than two times that of a flat plate structure, and the output can better meet the requirements of electrical stimulation treatment.

In this embodiment, a rectifier bridge 2 is disposed between the nanogenerator 1 and the stimulating electrode 3. When the AC voltage does not pass through the rectifier bridge 2, the AC voltage is an AC pulse electrical signal; the direct current pulse electrical signal is obtained through a rectifier bridge 2.

In this embodiment, the nano-generator 1 is a piezoelectric nano-generator, the nano-generator 1 includes a Kapton film 4, the Kapton film 4 is formed by thermal molding to form an arched Kapton film 4, the lower surface of the Kapton film 4 is sequentially covered with a first silver electrode 5 and a second silver electrode 6, and the first silver electrode 5 and the second silver electrode 6 are both electrically connected with the rectifier bridge 2 through a lead 7.

In this embodiment, the first silver electrode 5 is coated on the upper surface of a PVDF film 8, the second silver electrode 6 is coated on the lower surface of the PVDF film 8, and the PET film 9 is coated on the lower surface of the second silver electrode 6.

In this example, the Kapton film 4, the first silver electrode 5, the PVDF film 8, the second silver electrode 6, and the PET film 9 are bonded in sequence by a silicone polymer.

In this embodiment, the manufacturing process of the nano-generator 1 is as follows:

first, a heating rod is used to heat a substrate to a temperature of about 200 ℃ to a temperature of 55X 25X 0.1mm³The Kapton film 4 is thermoformed into an arch shape; after molding, the first silver electrode 5 and the second silver electrode 6 (each having a size of 50X 20X 0.11 mm) were placed³) Coating the upper surface and the lower surface of the PVDF film 8, and then attaching the PVDF film to the arched Kapton film 4; then, a PET film 9 was usedPackaging as a packaging layer; finally, the interlayer structures are bonded together one by a silicone polymer, producing a nanogenerator 1 (sm-PENG).

In this embodiment, the stimulating electrode 3 is a needle electrode. The stimulating electrode 3 comprises a positive electrode and a negative electrode, the negative electrode is connected with the wound broken end (fracture gap) of the affected part, and the positive electrode is connected with the adjacent muscle tissue of the wound broken end.

The current stimulation device 100 of the present embodiment stimulates osteogenic differentiation by self-powered pulse direct current, and the pulse DC of the nanogenerator 1 promotes the osteogenesis of MC3T3-E1, and also inhibits osteoclasts.

The current stimulation device 100 of the present embodiment can effectively promote osteoblast proliferation and promote intracellular calcium ions through the pulse DC generated by the rectifier bridge 2, and has a certain cell orientation effect. Meanwhile, the cell ALP activity can be promoted under the long-term culture condition, and finally calcium deposition, extracellular matrix mineralization and osteogenic differentiation are promoted. The biological effect of the pulse direct current stimulation of the nano generator 1 is basically consistent with that of a commercial signal generator. The embodiment provides a new idea for fracture healing, and promotes the application development of the nano generator 1 in wearable electronic medical equipment.

Example two

The difference between this embodiment and the first embodiment is: the nano-generator 1 is a friction nano-generator.

EXAMPLE III

As shown in fig. 4, the present embodiment provides a system 200 for current-stimulated osteoblast differentiation therapy, including the current stimulation device 100 of the first embodiment or the second embodiment, wherein the connection structures 10 are disposed at two ends of the nano-generator 1, the connection structures 10 are straps, and the connection structures 10 are connected to a splint. The connection structure 10 may be connected to the gauze, and then the gauze may be connected to the splint, and the current stimulation apparatus 100 may be combined with the splint to form the current-stimulated osteoblast differentiation therapy system 200, which has good flexibility and portability.

The principle and the implementation mode of the present invention are explained by applying specific examples in the present specification, and the above descriptions of the examples are only used to help understanding the method and the core idea of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.