阅读说明：本技术 一种防治植物细菌性病害的中药提取物及其应用 (Traditional Chinese medicine extract for preventing and treating plant bacterial diseases and application thereof ) 是由 徐凤武 曾雨良 车艳哲 于 2021-02-04 设计创作，主要内容包括：本发明公开了一种防治植物细菌性病害的中药提取物及其应用,所述中药提取物为艾叶黄酮类提取物,大田药效试验证明艾叶黄酮类提取物具有抑菌活性,该提取物浓度在500mg/L时,对柑橘溃疡病、火龙果溃疡病、桃树流胶病、猕猴桃溃疡病等细菌性病害有较好的防效,抑制率均在70％以上,为植物细菌性病害防治提供了一种新型、环保药剂。(The invention discloses a traditional Chinese medicine extract for preventing and treating plant bacterial diseases and application thereof, wherein the traditional Chinese medicine extract is a folium artemisiae argyi flavonoid extract, field drug effect tests prove that the folium artemisiae argyi flavonoid extract has antibacterial activity, when the concentration of the extract is 500mg/L, the traditional Chinese medicine extract has better prevention effect on bacterial diseases such as citrus canker, dragon fruit canker, peach tree gummosis, kiwi fruit canker and the like, the inhibition rate is more than 70%, and a novel and environment-friendly medicament is provided for preventing and treating plant bacterial diseases.)

1. A traditional Chinese medicine extract for preventing and treating plant bacterial diseases is characterized in that the traditional Chinese medicine extract is a folium artemisiae argyi flavonoid extract.

2. The folium artemisiae argyi flavonoid extract according to claim 1, characterized in that: the preparation method comprises the following steps:

1) pulverizing folium Artemisiae Argyi into moxa;

2) adding 15-30 times of 70% ethanol, heating and refluxing for 2-4 times, and obtaining filtrate after 1.5-3 hours each time;

3) after the filtrate is diluted, adding metal salt, adjusting the pH value to be alkaline for complexing, and collecting precipitate after the complexing is finished;

4) dissolving the precipitate in 30-50% alcohol, regulating pH value to acidity, and decomplexing. Removing solvent, dissolving with methanol, filtering, and drying the filtrate to obtain folium Artemisiae Argyi flavonoid extract.

3. The folium artemisiae argyi flavonoid extract according to claims 1-2, which can be processed into one of a microemulsion, an aqueous solution, a wettable powder and an emulsifiable concentrate.

4. The pesticidal composition according to claim 3, characterized in that: the weight of the folium artemisiae argyi flavonoid extract accounts for 10-90% of the total weight, and the balance is an agriculturally acceptable pesticide auxiliary agent.

5. The use of the herbal extract and the pesticidal formulation thereof according to any one of claims 1 to 4 for the control of bacterial diseases in plants.

Technical Field

The invention belongs to the technical field of prevention and treatment of plant bacterial diseases, and particularly relates to a traditional Chinese medicine extract of folium artemisiae argyi flavonoid and application of a pesticide preparation thereof in prevention and treatment of plant bacterial diseases.

Background

Chemical pesticides play a great role in agricultural production, and in recent years, abuse of chemical pesticides occurs, so that target organisms have resistance and damage to ecological environment. Therefore, new low-toxicity, high-efficiency, green and environment-friendly pesticides are needed in the market. The botanical pesticide is derived from plants, is green and environment-friendly, has high degradability, is difficult for harmful organisms to generate resistance, and has the potential of becoming a novel pesticide. But the plant source pesticide is used alone, has slow effect and poor quick-acting property, and can improve the control effect by being compounded with chemical pesticide.

Plants with bacteriostatic and bactericidal activity are numerous and contain many developed Chinese herbal medicines. The folium artemisiae argyi has been used for preventing pestilence for thousands of years, and pharmacological research of modern medicine shows that the folium artemisiae argyi is a broad-spectrum antibacterial and antiviral drug and has a certain inhibition effect on viruses and bacteria. Scientific research shows that the main active ingredient of the folium artemisiae argyi is a flavonoid compound, so that the flavonoid compound in the folium artemisiae argyi is extracted and used as the main active ingredient of the botanical pesticide.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine extract containing folium artemisiae argyi flavonoid extract and a pesticide preparation thereof, which are used for preventing and treating plant bacterial diseases.

The traditional Chinese medicine extract is a folium artemisiae argyi flavonoid extract, is extracted from folium artemisiae argyi, and is prepared by the following steps:

1) pulverizing folium Artemisiae Argyi into moxa;

2) adding 15-30 times of 70% ethanol, heating and refluxing for 2-4 times, and obtaining filtrate after 1.5-3 hours each time;

3) after the filtrate is diluted, adding metal salt, adjusting the pH value to be alkaline for complexing, and collecting precipitate after the complexing is finished;

4) dissolving the precipitate in 30-50% alcohol, regulating pH value to acidity, and decomplexing. Removing solvent, dissolving with methanol, filtering, and drying the filtrate to obtain folium Artemisiae Argyi flavonoid extract.

The traditional Chinese medicine extract and the pesticide preparation thereof are realized by the following technical scheme:

the traditional Chinese medicine extract is a folium artemisiae argyi flavonoid extract, and can be processed into one of microemulsion, aqueous solution, wettable powder and missible oil.

The folium artemisiae argyi flavonoid extract accounts for 10-90% of the total weight, and the balance is an agriculturally acceptable pesticide auxiliary agent.

The pesticide adjuvant can specifically comprise one or more of wetting agent, dispersing agent, antifreezing agent, emulsifier, penetrating agent, preservative, stabilizer, defoaming agent, filler and organic solvent, and the specific components and the using amount are determined by experiments.

The wetting agent can be one or more selected from sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, sodium butyl naphthalene sulfonate, sodium alkyl sulfonate, dioctyl sodium sulfosuccinate and alkylphenol polyoxyethylene ether sulfosuccinate.

The dispersing agent can be one or more of naphthalene formaldehyde condensate sulfonate, benzene sulfonate, alkyl naphthalene formaldehyde condensate sulfonate, lignosulfonate, polycarboxylate, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether and phosphate.

The antifreeze can be one or more selected from ethylene glycol, propylene glycol, isopropanol, glycerol and urea.

The emulsifier can be one or more selected from alkylphenol polyoxyethylene, benzyl phenol polyoxyethylene, phenethyl phenol polyoxyethylene, fatty alcohol polyoxyethylene and its similar products, fatty amine polyoxyethylene, and polyoxyethylene polyoxypropylene block copolymer.

The penetrating agent can be one or more selected from siloxane polyoxyethylene ether, fatty alcohol, alcohol ether succinate and phenol ether succinate.

The preservative can be one or more selected from sodium benzoate, sorbic acid, formaldehyde, butyl p-hydroxybenzoate, potassium sorbate, sodium salicylate, and KASONG.

The stabilizer may be one or more selected from 2, 6-di-tert-butyl-4-methylphenol (BHT), citric acid and Butyl Hydroxy Anisol (BHA).

The defoaming agent can be one or more selected from C8-10 fatty alcohol, epoxidized soybean oil, methanol, silicone oil, polyether defoaming agent and organic silicone.

The filler can be one or more selected from diatomite, attapulgite, bentonite, light calcium carbonate, pottery clay, kaolin, white carbon black, talcum powder, clay, lignin, montmorillonite, corn starch, and ammonium sulfate.

The organic solvent can be one or more selected from alcohol, aromatic hydrocarbon, ketone, ether, fatty acid ester, aliphatic hydrocarbon and vegetable oil.

The Chinese medicinal extract and the pesticide preparation thereof can be used for preventing and treating plant bacterial diseases, particularly citrus canker, dragon fruit canker, peach gummosis and kiwi canker, but the pesticide composition can prevent and treat the plant bacterial diseases, and specific diseases of the pesticide composition can also include, but are not limited to cucumbers (such as bacterial angular leaf spot, rhizoctonia solani and leaf blight), watermelons (such as bacterial angular leaf spot), tomatoes (such as bacterial wilt, ulcer, scab and bacterial leaf eruption), peppers (such as bacterial wilt, scab, soft rot and bacterial leaf spot), Chinese cabbages (such as cabbage soft rot, black rot, Chinese cabbage bacterial angular leaf spot and leaf spot), cabbages (such as cabbage soft rot, black rot and bacterial black spot), potatoes (such as bacterial wilt, ring rot, bacterial black scab and leaf spot), and the like, Soft rot), kidney beans (e.g.: bacterial blight, blight), eggplant (e.g.: bacterial wilt), peach (e.g.: bacterial perforation disease).

The invention takes pesticide extract-folium artemisiae argyi flavonoid extract as a novel material for preventing and treating bacterial diseases, and prepares the pesticide formulation for preventing and treating the bacterial diseases of plants. The antibacterial pesticide has the characteristics of no pollution, easy degradation and environmental friendliness, pathogenic bacteria are not easy to generate drug resistance, and the generation of the drug resistance of the pathogenic bacteria can be delayed.

Detailed Description

The following description is given with reference to specific embodiments, which are only exemplary and do not limit the scope of the present invention in any way. Any obvious modifications thereto, which may occur to those skilled in the art, are to be considered within the scope of the invention as defined by the appended claims.

Example 1: preparation of 90% folium artemisiae argyi flavonoid extract wettable powder

90% of folium artemisiae argyi flavonoid extract, 8% of sodium dodecyl benzene sulfonate and 2% of sodium lignosulphonate. Mixing the effective components with other adjuvants, mechanically pulverizing, jet milling, and mixing to obtain 90% wettable powder of folium Artemisiae Argyi flavonoid extract.

Example 2: preparation of 10% folium Artemisiae Argyi flavonoid extract aqua

10% of folium artemisiae argyi flavonoid extract, 5% of sodium dodecyl benzene sulfonate and 85% of water are uniformly mixed to prepare a 10% folium artemisiae argyi flavonoid extract aqueous solution.

Example 3: preparation of microemulsion containing 25% folium Artemisiae Argyi flavonoid extract

25% of folium artemisiae argyi flavonoid extract, 10% of aromatic hydrocarbon, 8% of alkylphenol polyoxyethylene, 5% of alcohol and 52% of water. Dissolving folium Artemisiae Argyi flavonoid extract in aromatic hydrocarbon, mixing with alkylphenol polyoxyethylene and alcohol, slowly adding 52% water, stirring well for 30 min to obtain 25% folium Artemisiae Argyi flavonoid extract microemulsion.

Example 4: preparation of 50% folium artemisiae argyi flavonoid extract missible oil

50% of folium artemisiae argyi flavonoid extract, 15% of aromatic hydrocarbon, 5% of vegetable oil, 20% of fatty alcohol-polyoxyethylene ether and 10% of alkylphenol polyoxyethylene ether are uniformly mixed to prepare 50% of folium artemisiae argyi flavonoid extract missible oil.

Application example 1:

field efficacy test of wettable powder containing 190% folium artemisiae argyi flavonoid extract for preventing and treating citrus canker

The test results (table 1) show that the wettable powder of the flavonoid extract of folium artemisiae argyi has better control effect on citrus canker.

Application example 2:

field efficacy test of aqueous solution containing folium artemisiae argyi flavonoid extract with content of Table 210% for preventing and treating peach tree gummosis

The test results (table 2) show that the 10% folium artemisiae argyi flavonoid extract aqua has better prevention effect on peach tree gummosis.

Application example 3:

field efficacy test of microemulsion containing 325% folium artemisiae argyi flavonoid extract for preventing and treating dragon fruit canker

The test results (table 3) show that the microemulsion containing 25% of folium artemisiae argyi flavonoid extract has a good prevention effect on the dragon fruit canker.

Application example 4:

field efficacy test of table 450% folium artemisiae argyi flavonoid extract missible oil for preventing and treating kiwifruit canker

The test results (table 4) show that the 50% folium artemisiae argyi flavonoid extract missible oil has a good prevention effect on the kiwifruit canker.