阅读说明：本技术 一种抗rna病毒的纳米薄膜及其制备方法 (anti-RNA virus nano-film and preparation method thereof ) 是由 黄方平 于 2021-02-24 设计创作，主要内容包括：本发明涉及一种抗RNA病毒的纳米薄膜及其制备方法,按重量份计,包括薄膜粒料95-97份,负离子纳米矿物粉末0.5-1份,中药材提取物1-2份,纳米沸石粉末2-3份；薄膜粒料为PE、PVC、PET等基材之一；中药材提取物包含的中药材按重量份为连翘8-10份、金丝桃8-10份、山慈菇10-12份、金银花6-8份、大青叶6-8份、黄芩5-8份、贯众4-6份、黄芪4-6份、板兰根3-5份、紫花地丁2-4份、白头翁2-4份、大黄2-4份；通过在薄膜生产过程中加入上述活性抗病毒成分,实现体外抗RNA病毒效果,并通过不同基材薄膜实现对公共场所不同触摸部位的全面防护,做到了公共场所接触传播的全方位实时消杀。(The invention relates to an anti-RNA virus nano-film and a preparation method thereof, which comprises 95 to 97 parts of film granules, 0.5 to 1 part of negative ion nano mineral powder, 1 to 2 parts of Chinese medicinal material extract and 2 to 3 parts of nano zeolite powder by weight; the film granules are one of the base materials such as PE, PVC, PET and the like; the traditional Chinese medicine extract comprises, by weight, 8-10 parts of fructus forsythiae, 8-10 parts of hypericum, 10-12 parts of edible tulip, 6-8 parts of honeysuckle, 6-8 parts of folium isatidis, 5-8 parts of scutellaria baicalensis, 4-6 parts of cyrtomium rhizome, 4-6 parts of astragalus membranaceus, 3-5 parts of isatis root, 2-4 parts of Chinese violet, 2-4 parts of Chinese pulsatilla root and 2-4 parts of rheum officinale; the active antiviral components are added in the film production process, so that the in-vitro anti-RNA virus effect is realized, the comprehensive protection of different touch parts in public places is realized through different base material films, and the comprehensive real-time disinfection and killing of contact propagation in public places are realized.)

1. An anti-RNA virus nano-film is characterized by comprising the following components in parts by weight: 95-97 parts of film granules, 0.5-1 part of negative ion nano mineral powder, 1-2 parts of traditional Chinese medicine extract and 2-3 parts of nano zeolite powder;

the film granules are one of PE, PVC, PET, LDPE, LHPE and PTFE substrate granules;

the traditional Chinese medicine extract comprises, by weight, 8-10 parts of fructus forsythiae, 8-10 parts of hypericum, 10-12 parts of edible tulip, 6-8 parts of honeysuckle, 6-8 parts of folium isatidis, 5-8 parts of scutellaria baicalensis, 4-6 parts of cyrtomium rhizome, 4-6 parts of astragalus membranaceus, 3-5 parts of isatis root, 2-4 parts of philippine violet herb, 2-4 parts of Chinese pulsatilla root and 2-4 parts of rheum officinale.

2. A preparation method of an anti-RNA virus nano-film is characterized by comprising the following steps:

s1, extracting the essence of the traditional Chinese medicinal materials:

firstly, weighing the traditional Chinese medicine part in the first aspect of the invention according to the parts by weight;

secondly, extracting fructus forsythiae, hypericum, honeysuckle, scutellaria baicalensis, astragalus mongholicus, Chinese violet and rhubarb from the Chinese medicinal material part weighed in the first step by adopting a supercritical carbon dioxide extraction technology, firstly, cleaning and airing the fructus forsythiae, the hypericum, the honeysuckle, the scutellaria baicalensis, the astragalus mongholicus, the Chinese violet and the rhubarb, crushing a single dose of the Chinese medicinal material, adding 50% ethanol for soaking for 1 hour, then sequentially putting the Chinese medicinal material into supercritical carbon dioxide extraction equipment for extraction, fully mixing the Chinese medicinal material and the crushed Chinese medicinal material to obtain a target extract containing the antiviral active ingredients of the Chinese medicinal materials such as flavonoid, polysaccharide and derivative, triterpenoid and derivative, alkaloid, glycoside and the like, then filtering the target extract, and placing the treated extract into a sterile container for storage;

thirdly, sequentially cleaning, crushing, drying and grinding the edible tulip, the indigowoad leaf, the cyrtomium rhizome, the isatis root and the Chinese pulsatilla root in the Chinese medicinal part weighed in the first step into nano-scale powder, fully mixing the nano-scale powder, and putting the nano-scale powder into a sealed glass container for later use;

s2, preparation of anti-RNA virus preparation:

firstly, weighing the components of the negative ion nano mineral powder and the nano zeolite powder in the first aspect of the invention according to the weight parts;

secondly, putting water with the weight part of 8-10 times of the sum of the components weighed in the first step into a glass or ceramic stirrer in a sterile environment, adding the negative ion nano mineral powder weighed in the first step, then stirring for 20-30 minutes in a closed manner, and placing the mixture into a closed glass or ceramic container;

thirdly, adding the biological essences extracted in the second step and the third step in the S1 into the glass or ceramic container under the aseptic environment, and fully stirring and mixing;

fourthly, adding the nano zeolite powder weighed in the first step into the glass or ceramic container, fully soaking for 1-2 hours, and then drying at the temperature below 85 ℃ until the water content is less than or equal to 8 percent to prepare a preparation for resisting RNA viruses;

s3, preparation of an anti-RNA virus nano-film:

step one, weighing the film granule component of the first aspect of the invention according to the weight parts;

and secondly, melting the film granules at high temperature, adding the anti-RNA virus preparation prepared in the step S2, fully stirring, and then sequentially plasticizing, extruding, blowing, drawing and coiling to obtain the anti-RNA virus nano film.

Technical Field

The invention belongs to the field of hygiene and epidemic prevention, and particularly relates to an anti-RNA virus nano-film and a preparation method thereof.

Background

RNA virus is a kind of virus, belonging to the first class; their genetic material is ribonucleic acid (RNA ribonucleic acid). The single-stranded RNA virus can be divided into positive-translation, negative-translation and double-translation RNA viruses according to the translation significance, and the positive-translation RNA virus is similar to mRNA and can be directly translated into protein by ribosome; negative-translation RNA viruses require the action of RNA polymerase, which synthesizes a positive-translation RNA complementary to itself using itself as a template, and then translates proteins using this RNA as mRNA.

RNA virus is mistakenly spelled during replication, the mutation speed of RNA virus is 100 ten thousand times faster than that of DNA virus, and the physique is changed frequently, so that new coronavirus and SARS virus belong to RNA virus.

Contact transmission is one of the transmission modes of RNA viruses, and currently, the pneumonia transmission routes of the novel coronavirus infection can be determined mainly as direct transmission, aerosol transmission and contact transmission;

contagious transmission refers to infection caused by sneezing, coughing and talking droplets deposited on the surface of the article, which contact the contaminated hands and then contact the mucous membranes of the mouth, nasal cavity, eyes, etc.

Aiming at the contact transmission of RNA viruses, public places are the first place to rush, and at present, a public place disinfection and killing preventive measure is advocated, and the measure achieves disinfection and killing of a certain area range, particularly public contact parts; however, the above-mentioned preventive measures are not complete and not timely, because the public place disinfection measures are not carried out in real time, each disinfection can only kill the existing virus and play a preventive effect in a short time, and once a patient infected with new corona virus leaves droplets on public contact parts such as elevator keys, elevator handrails, bus grippers, public door handles, faucet handles, bar counter tops, classroom table tops, etc., the above-mentioned preventive measures have the problem of untimely protection, and even some people without paying attention to personal hygiene can leave new corona virus on other occasions again;

therefore, in order to solve the above problems, it is urgent to invent an anti-RNA virus nano-film that can be used in public places to prevent contact transmission.

Besides defining the curative effect of the chloroquine phosphate on the novel pneumonia, a plurality of traditional Chinese medicines also show obvious antiviral effect in clinical and in-vitro efficacy experiments; the prescription of the granule for dispelling plague, named as 'pneumonia No. 1 prescription', comprises extracts of fructus forsythiae, fructus Rhodomyrti, Pseudobulbus Cremastrae seu pleiones, flos Lonicerae, Scutellariae radix, folium Isatidis, radix astragali, etc.;

in addition, the research on the in vitro antiviral pharmacodynamics of the 5 traditional Chinese medicine extracts (Yangjie, Liu Lian, Wu Xiao Yu; No. 5,375 one 376 in 2007) published in the journal of the academy of advancement and maintenance of military medicine specially discusses the in vitro antiviral action mechanism of the traditional Chinese medicine extracts of isatis root, astragalus root, cyrtomium rhizome, Chinese violet and rhubarb, and gives the conclusion that the 5 traditional Chinese medicines have stronger antiviral action.

The negative ions are not only often used for refreshing air, but also found through research of a southern mountain team that the negative ions can be combined with RNA viruses such as new coronavirus, SARS virus and the like to cause structural change or energy transfer of the RNA viruses, and finally the RNA viruses die.

In addition, the natural zeolite is a natural non-metallic mineral, is a safe environment-friendly new material which is determined by international scientific research institutions and can cover the whole industrial chain, is filled with fine holes and channels, has 100 ten thousand nanometer holes in 1 cubic micron, and has huge specific surface area; the unique molecular structure of the zeolite enables the zeolite to have the characteristics of excellent antiviral property, strong adsorbability, ion exchange property, catalytic property, acid and alkali resistance, high temperature resistance, corrosion resistance, molecular sieve function and the like.

Therefore, through research and development of four months, research and development personnel of the company add the traditional Chinese medicine extracts, the negative ion nano mineral powder, the nano zeolite powder and the nano titanium dioxide powder into the production process of the cast films of PE, PVC, PET, LDPE, LHPE, PTFE and the like to prepare the finished product, and can effectively resist RNA viruses such as new crown pneumonia, SARS, influenza and the like.

Disclosure of Invention

In order to solve the technical problems, the invention aims to provide an anti-RNA virus nano-film and a preparation method thereof, and the anti-RNA virus nano-film realizes the effect of the film with the anti-RNA virus function by adding traditional Chinese medicine extracts, negative ion nano-mineral powder and nano-zeolite powder in the production process of the traditional PE, PVC, PET, LDPE, LHPE, PTFE and other cast films, not only can effectively resist RNA virus in vitro and prevent virus contact propagation, but also can realize comprehensive protection on different touch parts in public places through films with different substrates, and can realize omnibearing real-time disinfection and killing of contact propagation in public places.

The first aspect of the invention provides an anti-RNA virus nano-film, which comprises the following components in parts by weight: 95-97 parts of film granules, 0.5-1 part of negative ion nano mineral powder, 1-2 parts of traditional Chinese medicine extract and 2-3 parts of nano zeolite powder;

the film granules are one of PE, PVC, PET, LDPE, LHPE and PTFE substrate granules;

the traditional Chinese medicine extract comprises, by weight, 8-10 parts of fructus forsythiae, 8-10 parts of hypericum, 10-12 parts of edible tulip, 6-8 parts of honeysuckle, 6-8 parts of folium isatidis, 5-8 parts of scutellaria baicalensis, 4-6 parts of cyrtomium rhizome, 4-6 parts of astragalus membranaceus, 3-5 parts of isatis root, 2-4 parts of philippine violet herb, 2-4 parts of Chinese pulsatilla root and 2-4 parts of rheum officinale.

The second aspect of the present invention provides a method for preparing an anti-RNA virus nano-film, comprising the following steps:

s1, extracting the essence of the traditional Chinese medicinal materials:

firstly, weighing the traditional Chinese medicine part in the first aspect of the invention according to the parts by weight;

secondly, extracting fructus forsythiae, hypericum, honeysuckle, scutellaria baicalensis, astragalus mongholicus, Chinese violet and rhubarb from the Chinese medicinal material part weighed in the first step by adopting a supercritical carbon dioxide extraction technology, firstly, cleaning and airing the fructus forsythiae, the hypericum, the honeysuckle, the scutellaria baicalensis, the astragalus mongholicus, the Chinese violet and the rhubarb, crushing a single dose of the Chinese medicinal material, adding 50% ethanol for soaking for 1 hour, then sequentially putting the Chinese medicinal material into supercritical carbon dioxide extraction equipment for extraction, fully mixing the Chinese medicinal material and the crushed Chinese medicinal material to obtain a target extract containing the antiviral active ingredients of the Chinese medicinal materials such as flavonoid, polysaccharide and derivative, triterpenoid and derivative, alkaloid, glycoside and the like, then filtering the target extract, and placing the treated extract into a sterile container for storage;

thirdly, sequentially cleaning, crushing, drying and grinding the edible tulip, the indigowoad leaf, the cyrtomium rhizome, the isatis root and the Chinese pulsatilla root in the Chinese medicinal part weighed in the first step into nano-scale powder, fully mixing the nano-scale powder, and putting the nano-scale powder into a sealed glass container for later use;

s2, preparation of anti-RNA virus preparation:

firstly, weighing the components of the negative ion nano mineral powder and the nano zeolite powder in the first aspect of the invention according to the weight parts;

secondly, putting water with the weight part of 8-10 times of the sum of the components weighed in the first step into a glass or ceramic stirrer in a sterile environment, adding the negative ion nano mineral powder weighed in the first step, then stirring for 20-30 minutes in a closed manner, and placing the mixture into a closed glass or ceramic container;

thirdly, adding the biological essences extracted in the second step and the third step in the S1 into the glass or ceramic container under the aseptic environment, and fully stirring and mixing;

fourthly, adding the nano zeolite powder weighed in the first step into the glass or ceramic container, fully soaking for 1-2 hours, and then drying at the temperature below 85 ℃ until the water content is less than or equal to 8 percent to prepare a preparation for resisting RNA viruses;

s3, preparation of an anti-RNA virus nano-film:

step one, weighing the film granule component of the first aspect of the invention according to the weight parts;

and secondly, melting the film granules at high temperature, adding the anti-RNA virus preparation prepared in the step S2, fully stirring, and then sequentially plasticizing, extruding, blowing, drawing and coiling to obtain the anti-RNA virus nano film.

The invention provides an anti-RNA virus nano-film and a preparation method thereof, compared with the prior art, the invention has the following beneficial effects:

1. according to the invention, traditional Chinese medicine extracts, negative ion nano mineral powder and nano zeolite powder are added in the production process of the traditional PE, PVC, PET, LDPE, LHPE, PTFE and other cast films, so that the effect of the film with the RNA virus resistance function is realized, the in-vitro RNA virus resistance can be effectively carried out, the virus contact propagation is prevented, the comprehensive protection of different touch parts in public places is realized through films of different base materials, and the omnibearing real-time disinfection and killing of the contact propagation in public places are realized;

2. according to the invention, zeolite is used as a carrier in the nano-film, so that not only are the traditional Chinese medicine extract and the negative ion nano mineral powder fully fused into the internal reticular structure in the preparation process of the anti-RNA virus preparation, but also the RNA virus can be adsorbed into the internal reticular structure almost without dead corners in the use process of the nano-film, and finally the RNA virus is thoroughly killed;

3. in the process of extracting the traditional Chinese medicine essence, two extraction modes are adopted, so that the combination degree of the traditional Chinese medicine essence and zeolite is further improved;

4. in the traditional Chinese medicine essence extraction process, a part of traditional Chinese medicines are extracted by adopting a supercritical carbon dioxide extraction technology, so that the defects of large loss of effective components, low yield, unstable quality and the like in the traditional extraction method are effectively overcome.

Detailed Description

In order that the objects and advantages of the invention will be more clearly understood, the invention is further described in detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Example 1:

an anti-RNA virus nano-film comprises the following components in parts by weight: 96 parts of film granules, 0.5 part of negative ion nano mineral powder, 1.5 parts of Chinese medicinal material extract and 2 parts of nano zeolite powder;

the film granules are PE granules;

the traditional Chinese medicine extract comprises the following traditional Chinese medicines, by weight, 10 parts of fructus forsythiae, 10 parts of hypericum, 10 parts of edible tulip, 8 parts of honeysuckle, 8 parts of folium isatidis, 8 parts of scutellaria baicalensis, 4 parts of cyrtomium rhizome, 4 parts of astragalus membranaceus, 4 parts of isatis root, 2 parts of Chinese violet, 2 parts of Chinese pulsatilla root and 2 parts of rheum officinale.

A preparation method of an anti-RNA virus nano-film comprises the following steps:

s1, extracting the essence of the traditional Chinese medicinal materials:

firstly, weighing the traditional Chinese medicine part in the first aspect of the invention according to the parts by weight;

secondly, extracting fructus forsythiae, hypericum, honeysuckle, scutellaria baicalensis, astragalus mongholicus, Chinese violet and rhubarb from the Chinese medicinal material part weighed in the first step by adopting a supercritical carbon dioxide extraction technology, firstly, cleaning and airing the fructus forsythiae, the hypericum, the honeysuckle, the scutellaria baicalensis, the astragalus mongholicus, the Chinese violet and the rhubarb, crushing a single dose of the Chinese medicinal material, adding 50% ethanol for soaking for 1 hour, then sequentially putting the Chinese medicinal material into supercritical carbon dioxide extraction equipment for extraction, fully mixing the Chinese medicinal material and the crushed Chinese medicinal material to obtain a target extract containing the antiviral active ingredients of the Chinese medicinal materials such as flavonoid, polysaccharide and derivative, triterpenoid and derivative, alkaloid, glycoside and the like, then filtering the target extract, and placing the treated extract into a sterile container for storage;

thirdly, sequentially cleaning, crushing, drying and grinding the edible tulip, the indigowoad leaf, the cyrtomium rhizome, the isatis root and the Chinese pulsatilla root in the Chinese medicinal part weighed in the first step into nano-scale powder, fully mixing the nano-scale powder, and putting the nano-scale powder into a sealed glass container for later use;

s2, preparation of anti-RNA virus preparation:

firstly, weighing the components of the negative ion nano mineral powder and the nano zeolite powder in the first aspect of the invention according to the weight parts;

secondly, putting water with the weight part being 8 times of the sum of the components weighed in the first step into a glass or ceramic stirrer in a sterile environment, adding the negative ion nano mineral powder weighed in the first step, then stirring for 25 minutes in a closed manner, and placing the mixture into a closed glass or ceramic container;

thirdly, adding the biological essences extracted in the second step and the third step in the S1 into the glass or ceramic container under the aseptic environment, and fully stirring and mixing;

fourthly, adding the nano zeolite powder weighed in the first step into the glass or ceramic container, fully soaking for 1 hour, and then drying at 85 ℃ until the water content is less than or equal to 8 percent to prepare a preparation for resisting RNA viruses;

s3, preparation of an anti-RNA virus nano-film:

step one, weighing the film granule component of the first aspect of the invention according to the weight parts;

and secondly, melting the film granules at high temperature, adding the anti-RNA virus preparation prepared in the step S2, fully stirring, and then sequentially plasticizing, extruding, blowing, drawing and coiling to obtain the anti-RNA virus nano film.

Example 2:

compared with the example 1, the film granules are replaced by the PE material into the PVC material, and other components and the preparation method are the same as the example 1.

Example 3:

compared with example 1, the film pellets in this example were replaced by PE material and PET material, and the other components and preparation method were the same as those in example 1.

Comparative example 1:

compared with the example 1, the comparative example has less traditional Chinese medicine extract components, and correspondingly lacks the preparation of biological extract essence in the preparation method, and other components and the preparation method are the same as the example 1.

Comparative example 2:

compared with the example 2, the comparative example has less traditional Chinese medicine extract components, and correspondingly lacks the preparation of biological extract essence in the preparation method, and other components and the preparation method are the same as the example 2.

Comparative example 3:

compared with the example 3, the comparative example has less traditional Chinese medicine extract components, and correspondingly lacks the preparation of biological extract essence in the preparation method, and other components and the preparation method are the same as the example 3.

Comparative example 1:

compared with the example 1, the composition of the nano film of the comparative example is 100% PE material, and does not contain any other formulation components.

Comparative example 2:

compared with the example 1, the nano-film component of the comparative example is 100% PVC material, and does not contain any other formulation components.

Comparative example 3:

compared with the example 1, the composition of the nano film of the comparative example is 100% of PET material, and does not contain any other formulation components.

Effect test: and (3) detecting the activity rate of the anti-new coronavirus:

inspecting samples: the nano-films prepared in examples 1 to 3, comparative examples 1 to 3 and comparative examples 1 to 3;

and (3) killing viruses: novel coronavirus (SARS-CoV-2) MDCK cells;

the detection method comprises the following steps: 3 parts of samples to be tested are divided into one group, the size of each group is 5mm x 5mm, novel coronavirus (SARS-CoV-2) MDCK cell inoculation is carried out under the constant temperature, constant humidity and sterile environment, after inoculation, the logarithm value of the virus titer is detected after 2 hours, and then the activity rate of resisting the new coronavirus is obtained;

the detection results are shown in table 1:

TABLE 1 determination of the Activity Rate against New coronavirus

And (4) conclusion:

1. as can be seen from Table 1, the antiviral activity rates of examples 1-3 all reached more than 99%, so that the replacement of the nano-film granules of the present invention could be obtained without affecting the antiviral activity rate of the nano-film prepared by the present invention; 2. after the components of the traditional Chinese medicine extract are removed, the antiviral activity rate of the comparative examples 1-3 is only maintained at about 46 percent, so that the active components of flavonoid, polysaccharide and derivatives, triterpenoid and derivatives, alkaloid, glycoside and the like in the traditional Chinese medicine extract can effectively resist new coronavirus; 3, the antiviral activity rate of the comparative examples 1-3 is only about 10%, and considering that RNA virus is killed automatically in a protein-free environment, the common film material has almost no antiviral capability, and meanwhile, the anion nano mineral powder can play a certain auxiliary antiviral role;

from the above 3 points, the Chinese medicinal material extract component of the invention can effectively resist RNA virus, and further improve the antiviral activity rate with the assistance of the anion nano mineral powder.

The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention to achieve the above technical effects.