阅读说明：本技术 一种抗病促长的中药复方及其制备和应用 (Traditional Chinese medicine compound for resisting diseases and promoting growth as well as preparation and application thereof ) 是由 郭世宁 刘梦杰 李越 吕伟杰 杜宏亮 晁利民 屈倩 王志华 张文嫦 于 2021-01-07 设计创作，主要内容包括：本发明提供了一种抗病促长的中药复方及其制备和应用。该中药复方中,马齿苋、苦参、马尾连、伏龙肝、广藿香的质量比为0.8～1.2：0.8～1.2：0.8～1.2：0.8～1.2：0.8～1.2,诸药相和,共奏抗菌消炎、清热燥湿、凉血解毒、止痢止泻、养阴生津、增强免疫力功效,可有效促进养殖动物的生长,同时该中药复方成本低廉、安全无耐药性、环保无残留、不含抗生素,整体上优于促生长抗生素,为无抗、绿色健康的养殖模式提供新的思路；此外,该中药复方的制备简单,不需要其他佐剂,能满足养殖业中大规模使用的需求。(The invention provides a traditional Chinese medicine compound for resisting diseases and promoting growth, and a preparation method and application thereof. In the traditional Chinese medicine compound, the mass ratio of the purslane, the radix sophorae flavescentis, the meadowrue root and rhizome, the furnace soil and the patchouli is 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2, the traditional Chinese medicine compound has the effects of resisting bacteria and diminishing inflammation, clearing heat and drying dampness, cooling blood and detoxifying, stopping dysentery and checking diarrhea, nourishing yin and promoting the secretion of saliva or body fluid and enhancing immunity, can effectively promote the growth of cultured animals, is low in cost, safe, free of drug resistance, environment-friendly, free of residue, free of antibiotics, superior to growth-promoting antibiotics on the whole, and provides a new idea for a non-antibiotic, green and healthy culture mode; in addition, the traditional Chinese medicine compound is simple to prepare, does not need other adjuvants, and can meet the requirement of large-scale use in the breeding industry.)

1. The traditional Chinese medicine compound for resisting diseases and promoting growth is characterized by comprising purslane, radix sophorae flavescentis, meadowrue root and rhizome, fuling liver and cablin potchouli herb in a mass ratio of 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8 to 1.2.

2. The traditional Chinese medicine compound of claim 1, wherein the mass ratio of the purslane, the radix sophorae flavescentis, the meadowrue root and rhizome, the furnace soil and the patchouli is 1: 1: 1: 1: 1.

3. the compound Chinese medicine as claimed in claim 1 or 2, wherein the compound Chinese medicine is in the form of powder, tablet, capsule or decoction.

4. The preparation method of the traditional Chinese medicine compound of claim 1 or 2, which is characterized in that the traditional Chinese medicine compound is powder, and the traditional Chinese medicine compound is prepared by weighing the traditional Chinese medicine raw materials in parts by weight, crushing, drying and uniformly mixing.

5. The preparation method of claim 4, wherein the traditional Chinese medicine raw materials are ground and sieved by a sieve of 80-150 meshes.

6. The method of claim 4, wherein the drying is drying at 60 ℃.

7. The use of the herbal compound of claim 1 or 2 in the preparation of feed.

8. The use of the herbal compound of claim 1 or 2 in the preparation of a feed additive.

9. The application of claim 7, wherein the addition amount of the traditional Chinese medicine compound is 0.1-0.3% of the weight of the feed.

10. The use of claim 9, wherein the amount of the Chinese herbal compound is 0.1% of the weight of the feed.

Technical Field

The invention relates to the technical field of veterinary medicines, and particularly relates to a traditional Chinese medicine compound for resisting diseases and promoting growth, and preparation and application thereof.

Background

Growth-promoting antibiotics are added into feed for a long time in the breeding industry, on one hand, the opportunity of drug resistance transmission is expanded, a large number of livestock and poultry and even human beings are exposed to infection risks caused by drug-resistant strains, and on the other hand, great hidden dangers are brought to food safety. For this reason, the No. 194 bulletin of the rural area of China agriculture stipulates: since 1/7/2020, all growth-promoting antibiotics will not be added to the feed, and the feed enterprises are not allowed to add veterinary prescription drugs (except traditional Chinese medicines) to the feed processed by the breeding enterprises.

Therefore, the application of the traditional Chinese medicine in the feed or the additive thereof for preventing and treating the diseases of the cultured animals and promoting the growth is more and more extensive, and the non-resistant, green and healthy culture mode has wide development prospect. The traditional Chinese medicine contains various nutritional ingredients such as protein, amino acid, vitamin, polysaccharide and the like, can effectively improve the growth performance of animals and enhance the digestion and absorption functions; meanwhile, the compound immunopotentiator contains various effective immunocompetent components, promotes phagocytic function of macrophages and conversion rate of lymphocytes, is beneficial to inhibiting bacteria, and has obvious enhancement effect on cellular immunity and humoral immunity of organisms. Although the disease-resistant effect of the traditional Chinese medicine product developed by natural plants is hardly better than that of antibiotics and chemical synthetic medicines, the traditional Chinese medicine greatly reduces the risk of drug resistance and avoids the generation of super bacteria, and the traditional Chinese medicine in China also has the advantages of rich resources, low price, small toxic and side effects, no drug resistance, difficult residue and the like. For example, patent CN104026386A provides a Chinese herbal medicine feed for improving the growth of laying hens and a preparation method thereof, including pine needles, paulownia leaves, codonopsis pilosula leaves, folium artemisiae argyi, astragalus membranaceus, spina date seeds, polygala tenuifolia, kelp, bighead atractylodes rhizome, radix bupleuri, purslane, radix astragali, realgar, wei stone, agastache rugosus, golden cypress, fructus forsythiae, pulsatilla chinensis and radix sophorae flavescentis, but the types of the used traditional Chinese medicines are as high as 19, the dosage is as high as 3% of the feed, the cost is relatively high, and the feed.

Therefore, a novel disease-resistant growth-promoting traditional Chinese medicine compound which is low in price, high in efficiency, safe, free of drug resistance, environment-friendly, free of residues and free of antibiotics is developed, and has an important application value in the animal breeding industry.

Disclosure of Invention

The invention aims to overcome the defects of feed and additives thereof in the prior art and provides a traditional Chinese medicine compound for resisting diseases and promoting growth, and preparation and application thereof. In the traditional Chinese medicine compound, the mass ratio of the purslane, the radix sophorae flavescentis, the meadowrue root and rhizome, the furnace soil and the patchouli is 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2, the medicines are combined, the effects of resisting bacteria and diminishing inflammation, clearing heat and drying dampness, cooling blood and detoxifying, stopping dysentery and checking diarrhea, nourishing yin and promoting the production of body fluid and enhancing immunity are achieved, the growth of cultured animals is promoted, and the feed additive also has the advantages of low cost, safety, no drug resistance, environmental friendliness, no residue and no antibiotics.

The invention aims to provide a traditional Chinese medicine compound for resisting diseases and promoting growth.

The invention also aims to provide a preparation method of the traditional Chinese medicine compound.

The invention also aims to provide application of the traditional Chinese medicine compound in preparing feed and additives thereof.

The above purpose of the invention is realized by the following technical scheme:

the invention provides a traditional Chinese medicine compound for resisting diseases and promoting growth, which comprises purslane, radix sophorae flavescentis, meadowrue root and rhizome, fuling liver and cablin potchouli herb in a mass ratio of 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8 to 1.2.

Wherein, the purslane has the functions of clearing away heat and toxic material, eliminating carbuncle and promoting urination, is suitable for dysentery, carbuncle swelling, stranguria and the like caused by damp-heat or heat toxin, also has antibacterial effect, has the inhibition effect on Shigella shigella and Shigella shigella, and also has certain inhibition effect on typhoid bacillus, colon bacillus and staphylococcus aureus; radix Sophorae Flavescentis is bitter in taste and cold in nature, has the effects of clearing heat and eliminating dampness, dispelling pathogenic wind and killing parasites by walking through heart, spleen, large intestine and kidney channels, descending with sexual subsidence, and promoting urination, and can be used for treating damp-heat dysentery, and is often used with herba Portulacae and herba Incarvilleae sinensis; radix et rhizoma Thalictri has effects of clearing heat, eliminating dampness, and removing toxic substance, and can be used for treating dysentery, enteritis, infectious hepatitis, common cold, measles, carbuncle, swelling, sore, furuncle, conjunctivitis, etc., wherein the root and stem of radix et rhizoma Rhei are common Chinese medicinal materials of Yunnan Bai nationality, and can be used for treating enteritis, dysentery, jaundice, conjunctival congestion, swelling and pain, etc.; the fulonggan is pungent in property, slightly warm, and has the effects of warming middle-jiao and stomach, stopping diarrhea and stopping bleeding, is used for stomach cold and vomiting, abdominal pain and diarrhea, pernicious vomiting, hematemesis and hemafecia, menorrhagia and the like, contains various oxides and ions, can astringe and stop bleeding, warm middle-jiao and stop vomiting and stop diarrhea, achieves the effects of regulating the microecological structure and composition of intestinal tracts, further maintains immune balance, and further has the effects of preventing and treating diseases; patchouli is pungent in flavor, slightly warm in nature, and has the functions of regulating the middle warmer, relieving vomiting and relieving summer-heat by virtue of spleen, stomach and lung channels, is used for treating symptoms such as damp turbidity obstruction in middle warmer, epigastric fullness, vomiting, summer-heat-dampness exterior syndrome, early damp-warm syndrome, fever, lassitude, chest distress, discomfort, cold-dampness, summer-heat retention, abdominal pain, vomiting and diarrhea, nasosinusitis and headache and the like, contains about 1.5% of volatile oil, contains patchouli alcohol serving as a main chemical component in oil, and also contains sesquiterpene, and mainly has the effects of resisting bacteria, resisting viruses, resisting inflammation, relieving fever, easing pain and promoting gastric secretion.

The traditional Chinese medicine compound has the advantages of low cost, safety, no drug resistance, environmental protection, no residue and no antibiotic, and has the effects of resisting bacteria and diminishing inflammation, clearing heat and drying dampness, cooling blood and removing toxicity, stopping dysentery and checking diarrhea, nourishing yin and promoting the production of body fluid and enhancing immunity.

Repeated tests show that the mass ratio of the purslane, the radix sophorae flavescentis, the meadowrue root and rhizome, the furnace soil and the patchouli is 1: 1: 1: 1: 1.

the results of experiments on yellow-feathered broilers by using the traditional Chinese medicine compound show that the performance of an experimental group of the broilers eating the feed added with the traditional Chinese medicine compound in the aspects of average daily gain, total gain, feed conversion rate, ratio of small intestine villus density to crypt depth, spleen index, bursa of fabricius index, thymus index and the like is superior to or equal to that of a control group of the broilers eating the feed added with antibiotics, and the traditional Chinese medicine compound does not add antibiotics, can not cause drug resistance, can not expose a large amount of livestock and poultry and even human beings to infection risks caused by drug-resistant strains, and can not bring hidden dangers to food safety, so the traditional Chinese medicine compound is superior to growth-promoting antibiotics on the whole, and can provide a new thought for a nonreactive, green and healthy breeding mode.

Preferably, the dosage form of the traditional Chinese medicine compound is available dosage forms of animal medicines such as powder, tablets, capsules and decoction.

The medicament for the cultured animals has a plurality of applicable formulations, but in the breeding industry, the medicament is often used as powder and added into feed or feed additives for the animals to eat directly, mainly because the powder has simple preparation method, is easy to disperse in the body, has quick effect and is suitable for taking.

When the Chinese herbal compound is powder, the preparation method comprises the steps of weighing the Chinese herbal raw materials in parts by weight, crushing, drying and uniformly mixing.

The powder is simple in preparation process, low in cost of required raw materials, free of hormone and antibiotics, in line with the trend of future antibiotic reduction and environmental protection in the industry, and has great practical significance for a green and healthy breeding mode.

Preferably, the traditional Chinese medicine raw materials are ground and sieved by a sieve of 80-150 meshes.

Preferably, the drying is drying at 60 ℃.

The invention also provides application of the traditional Chinese medicine compound in preparing feed or feed additives.

Preferably, the addition amount of the traditional Chinese medicine compound is 0.1-0.3% of the weight of the feed.

Most preferably, the addition amount of the traditional Chinese medicine compound is 0.1% of the weight of the feed.

When the addition amount of the traditional Chinese medicine compound is 0.1-0.3 percent of the feed dosage, especially 0.1 percent, the weight of the cultured animals is increased most, the feed conversion rate is optimal, and the traditional Chinese medicine compound feed has optimal disease-resistant growth-promoting effect.

Compared with the prior art, the invention has the beneficial effects that:

in the traditional Chinese medicine compound, the medicines are combined, so that the effects of resisting bacteria and diminishing inflammation, clearing heat and drying dampness, cooling blood and detoxifying, stopping dysentery and checking diarrhea, nourishing yin and promoting the production of body fluid and enhancing immunity are achieved, the traditional Chinese medicine compound has the effect of promoting the growth of cultured animals, and meanwhile, the traditional Chinese medicine compound has the advantages of low cost, safety, no drug resistance, environmental protection, no residue and no antibiotic, is superior to growth-promoting antibiotics on the whole, and provides a new idea for a non-antibiotic, green and healthy culture mode. In addition, the traditional Chinese medicine compound is simple to prepare, does not need other adjuvants, and can meet the requirement of large-scale use in the breeding industry.

Drawings

Fig. 1 is a diagram of jejunum intestinal mucosa tissues of various groups of broilers.

Detailed Description

The invention will be further described with reference to the drawings and the detailed description, which are not intended to limit the invention in any way. The starting materials used in the examples of the present invention are conventionally purchased raw materials unless otherwise specified.

Example 1

1. Experimental Material

The traditional Chinese medicine compound group: according to the mass ratio of 1: 1: 1: 1: 1, weighing the raw materials of purslane, radix sophorae flavescentis, meadowrue root and rhizome, fuling and patchouli, crushing, drying in a hot air drying oven at 60 ℃, sieving with a 80-mesh sieve, uniformly mixing to prepare a traditional Chinese medicine compound, dividing into two groups, and respectively and uniformly mixing with feed to ensure that the addition amount of the traditional Chinese medicine compound in the feed is 1g/kg (traditional Chinese medicine compound/feed) and 3g/kg (traditional Chinese medicine compound/feed).

Antibiotic group (aureomycin 250 mg/kg): the aureomycin and the feed are mixed evenly to ensure that the mass ratio of the aureomycin to the feed is 250mg/kg (aureomycin/feed).

2. Experimental methods

Selecting 100 yellow-feathered broilers of 1 day old, dividing the broilers into four groups, namely a blank group (only feed), an antibiotic group (250 mg/kg) of aureomycin, a Chinese herbal compound group 1(1g/kg) and a Chinese herbal compound group 2(3g/kg), weighing, calculating the average empty stomach weight of each group, feeding the broilers for 42 days respectively, and freely drinking water during the period to ensure good ventilation and proper temperature. After feeding for 42 days, recording feed intake, weighing on an empty stomach, and calculating Average Daily Gain (ADG), total gain and feed-weight ratio (F/G) of the broilers in each group; quickly taking the middle section of the jejunum of 2-3 mm, immersing the middle section of the jejunum into prepared normal saline, rinsing off the content, adding 20mL of 4% paraformaldehyde phosphate buffer, fixing for 48h (keeping the pH value equal to 7.4), embedding by using conventional paraffin, preparing 10 continuous transverse sections (the thickness is 5 mu m), staining by conventional hematoxylin-eosin (H.E.), measuring the villus height and the crypt depth of the small intestine, and calculating the ratio of the villus height and the crypt depth; spleen, thymus and bursa of fabricius were collected, peripheral fat was removed and water was sucked off with filter paper, and then weighed and recorded, and immune organ index [ immune organ index (g/kg) ═ fresh weight (g) of immune organ/live weight (kg) ] was calculated.

3. Results of the experiment

The experimental results are shown in 1-5

TABLE 1 average daily gain of broiler chickens results (n＝25)

As can be seen from Table 1, compared with the blank group, the antibiotic group (250 mg/kg of aureomycin), the traditional Chinese medicine compound group 1(1g/kg) and the traditional Chinese medicine compound group 2(3g/kg) have certain effects on daily weight gain of the broiler chickens, wherein the traditional Chinese medicine compound group 1(1g/kg) has more obvious effect on daily weight gain of the broiler chickens, and can improve the average daily weight gain of the broiler chickens.

TABLE 2 Total broiler gain results

As shown in Table 2, compared with the blank group, the antibiotic group (250 mg/kg of aureomycin), the traditional Chinese medicine compound group 1(1g/kg) and the traditional Chinese medicine compound group 2(3g/kg) have certain effects on increasing the weight of the broilers in the whole period, wherein the traditional Chinese medicine compound group 1(1g/kg) has more obvious weight increasing effect on promoting the growth of the broilers in the whole period, and the difference is obvious (P is less than 0.05).

TABLE 3 broiler feed weight ratio results

As can be seen from Table 3, compared with the blank group, the weight ratio of the antibiotic group (250 mg/kg of aureomycin), the Chinese herbal compound group 1(1g/kg) and the Chinese herbal compound group 2(3g/kg) is obviously reduced, and the feed conversion rate is high. Wherein, the traditional Chinese medicine compound group 1(1g/kg) has more obvious effect of promoting the feed conversion of the broiler chickens (P is less than 0.05).

TABLE 4 index values of digestive absorption function of small intestine of broiler chicken

The absorption and utilization of the animal to the nutrient substances can be directly influenced by the quality of the intestinal mucosa structure, and the ratio of the villus height of the small intestine to the depth of the crypt is an important index for measuring the digestive absorption function of the small intestine. As can be seen from the observation of the data in the figure 1 and the analysis table 4, compared with the antibiotic group (250 mg/kg of aureomycin), the broiler chicken small intestine crypt depth of the traditional Chinese medicine compound group 1(1g/kg) and the broiler chicken small intestine crypt depth of the traditional Chinese medicine compound group 2(3g/kg) is reduced, and the villus height and the V/C value (small intestine villus height/small intestine crypt depth) are both improved to a certain extent, namely, the intestinal mucosa structure can be improved, the small intestine microvilli density is increased, the small intestine villus height is increased, the small intestine absorption area is increased, the intestinal mucosa crypt depth and the intestinal mucosa epithelial cell function are maintained, and the absorption and utilization of nutrient substances are facilitated, so that the growth of animals is promoted. Wherein, the Chinese herbal compound group 1(1g/kg) has the best effect of promoting digestion and absorption, the depth of crypt of small intestine is reduced, and the height of villus and the V/C value are both obviously improved (P is less than 0.05).

From the table 1-4, it can be seen that after the feed added with the traditional Chinese medicine compound is eaten, the weight increase, the feed conversion rate and the digestion and absorption functions of the broiler chickens are all obviously improved, and when the addition amount of the traditional Chinese medicine compound is 0.1% of the feed amount, the effect is optimal, and the increase of the broiler chickens can be obviously promoted.

TABLE 5 broiler immune organ index results

Spleen, thymus and bursa of fabricius are organs which exert immune effect on poultry organisms, and the immune index of the organs can be detected to preliminarily measure the immune state of the organisms and the development condition of an immune system. As can be seen from Table 5, spleen index, thymus index and bursa of Fabricius index of the antibiotic group (250 mg/kg of aureomycin), the Chinese herbal compound group 1(1g/kg) and the Chinese herbal compound group 2(3g/kg) are all improved to a certain extent, which belong to the increase within the normal range, reflect the enhancement of animal immune function to a certain extent, and are beneficial to promoting the organism to generate immune cells and improving the level of vaccine antibodies. The spleen index and the thymus index of the traditional Chinese medicine compound group 1(1g/kg) are obviously increased, the bursa of Fabricius index of the traditional Chinese medicine compound group 2(3g/kg) is obviously increased (P is less than 0.05), and the feed added with the traditional Chinese medicine compound has the effect of obviously improving the cellular immunity and the humoral immunity of the broiler.

In conclusion, the performances of the broiler experimental group eating the feed added with the traditional Chinese medicine compound in the aspects of average daily gain, total gain, feed conversion rate, ratio of small intestine villus density to crypt depth, spleen index, bursa of fabricius index, thymus index and the like are superior to or equal to those of a broiler control group eating the feed added with antibiotics, and the traditional Chinese medicine compound does not add antibiotics, does not cause drug resistance, does not expose a large amount of livestock and poultry and even human beings to infection risks caused by drug-resistant strains, and does not bring hidden dangers to food safety, so the traditional Chinese medicine compound is superior to growth-promoting antibiotics on the whole, and can provide a new idea for a non-resistant, green and healthy breeding mode.

In addition, through repeated experiments of the inventor, when the mass ratio of the purslane, the radix sophorae flavescentis, the meadowrue root and rhizome, the furnace soil and the patchouli is 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2, under the condition of the same total compound dosage, different proportions have little influence on experimental results, so the embodiment of the invention details that when the mass ratio is 1: 1: 1: 1: experimental results at 1.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.