阅读说明：本技术 一种全雄罗氏沼虾快速生长品系的选育方法 (Breeding method of all-male macrobrachium rosenbergii rapid-growth strain ) 是由 徐宾朋 陈雪峰 高强 彭菲 程海华 于 2020-12-31 设计创作，主要内容包括：本发明公开了一种全雄罗氏沼虾的家系选育方法,以缅甸系罗氏沼虾为基础群体,结合RNAi干扰制备“假雌”虾技术,建立全雄罗氏沼虾品系；以罗氏沼虾南太湖2号养殖群体为基础,结合其他收集群体组建家系,运用ASReml软件,根据BLUP法估算出每尾虾的育种值并排序；进行个体选育、家系选育和家系内选育；每代共建立200个以上的家系；选取优秀的雄虾和雌虾作下一代种虾,建立第二代选育家系；家系传代5年以后,每年从家系中选取优质雄虾作为父本,与“假雌”虾进行交配繁殖,最后获得快速生长的全雄品系；选育的全雄品系兼具双亲的优良性状,也可以避免近交衰退,获得的全雄罗氏沼虾生长快、品质优,适合低密度、大规格的养殖。(The invention discloses a family breeding method of full-male macrobrachium rosenbergii, which is characterized in that macrobrachium rosenbergii of Burma is taken as a basic group, a technology of preparing 'false female' macrobrachium by combining RNAi interference is combined, and a full-male macrobrachium rosenbergii strain is established; establishing a family by combining other collected groups based on the macrobrachium rosenbergii south Tai lake No. 2 breeding group, and estimating the breeding value of each shrimp and sequencing by using ASReml software according to a BLUP method; carrying out individual breeding, family breeding and family internal breeding; more than 200 families are built in each generation; selecting excellent male shrimps and excellent female shrimps as next generation of breeding shrimps, and establishing a second generation of breeding families; after the passage of the family for 5 years, selecting high-quality male shrimps from the family every year as male parents, and mating and breeding the male shrimps with the false female shrimps to finally obtain a fast-growing all-male strain; the bred all-male strain has the excellent properties of parents, inbreeding depression can be avoided, and the obtained all-male macrobrachium rosenbergii grows fast and has excellent quality and is suitable for low-density and large-size culture.)

1. A breeding method of a rapid growth strain of all-male macrobrachium rosenbergii is characterized by comprising the following steps:

step one, obtaining a female parent of an all-male strain:

taking Mycoplasma maindroni as a basic population, interfering a DMRT1 gene by combining RNAi to prepare 'false female' shrimp male ZZ, mating and breeding the 'false female' shrimp and normal male shrimp to generate full male offspring, and establishing a full male Macrobrachium rosenbergii strain; preparing the 'false female' shrimp every year as a subsequent female parent;

step two, the systematic breeding of male parent parents:

a, preparing a source of a basic breeding group:

collecting high-quality macrobrachium rosenbergii resources on the basis of macrobrachium rosenbergii germplasm resource general survey, identification and genetic rule analysis in a macrobrachium rosenbergii main production area, and establishing a macrobrachium rosenbergii breeding heart group by taking macrobrachium rosenbergii No. 2 in the south Taihu lake as a basic group;

b, constructing a family:

adopting directional mating of unbalanced nest type design to construct a full/half-sib family, and taking each female shrimp as a family after spawning;

when each family is independently standardized and cultured to 3cm, performing pond-sharing growth and survival test after each family is marked with VIE;

when the individual grows to be a shrimp and reaches the market specification, measuring the weight value of the individual, counting the survival rate of each family and measuring growth data;

c, data sorting and data analysis:

carrying out preliminary statistical analysis on the obtained character data, and calculating the average value, standard deviation and variation coefficient parameters of the characters;

d, using a mixture model, estimating by a BLUP method in combination with a REML method:

calculating genetic progress aiming at single sex, estimating selection reaction of a good group and a control group by adopting a general linear model for growth data, and calculating to realize genetic progress;

e, designing a next generation mating scheme:

the principles for establishing a mating scheme comprise:

the inbreeding coefficient of the genetic relationship between the individuals to be matched is less than 0.01;

selecting individuals with high breeding value in families according to the breeding value of the individuals, and randomly matching the families;

the number of individuals selected for each family; sex ratio of male and female, the male and female ratio is 4: 1;

f, male parent system seed reserving and propagation expanding:

the seed reserving method comprises the following steps: after harvesting parent shrimps every year, performing inter-family selection and intra-family selection, and selecting families with high breeding value and intra-family individuals;

the propagation method comprises the following steps: performing secondary propagation on the parent strain;

step three, expanding propagation of high-quality all-male macrobrachium rosenbergii:

selecting high-quality female shrimps from the 'false female' macrobrachium rosenbergii as female parents, and selecting high-quality male shrimps from the second-stage expanded population of the BLUP population as female parents; the two are naturally mated and propagated, thereby obtaining the high-quality all-male strain.

2. The breeding method of the all-male macrobrachium rosenbergii rapid-growth strain according to claim 1,

b, constructing a family:

adopting directional mating of unbalanced nest type design to construct a full/half-sib family, and taking each female shrimp as a family after spawning;

when each family is independently standardized and cultured to 3cm, performing pond-sharing growth and survival test after each family is marked with VIE;

when the individual grows to be a shrimp and reaches the market specification, measuring the weight value of the individual, counting the survival rate of each family and measuring growth data;

comprehensively recording the production performance, body length, body weight, sex, mating species and genealogy of all individuals of the core group macrobrachium rosenbergii to form complete data information and realize genetic evaluation and inbreeding analysis of the macrobrachium rosenbergii;

the number of individuals tested each year is not less than 10000, and assuming that the male-female ratio of each family is 1:1, the seed reserving rate of female and male shrimps of each generation is 4% and 2% respectively.

3. The breeding method of the all-male macrobrachium rosenbergii rapid-growth strain according to claim 1,

d, using a mixture model, estimating by a BLUP method in combination with a REML method:

calculating genetic progress aiming at single sex, estimating selection reaction of a good group and a control group by adopting a general linear model for growth data, and calculating to realize genetic progress;

the genetic evaluation animal model of the single character has the following formula: y is_ij＝u+a_i+S_i+SP_j+cx_ij+f_j+e_ij(1)；

Wherein, y_ijDenotes the measurement of growth performance of the i-th individual, u denotes the overall mean, a_iRepresents the ith individual sports value, S_iIndicating the Effect of immobilization of the ith sex，SP_jShows the fixing effect of whether the female shrimp individual is oviferous or not in the pond, x_ijRepresenting the age of the family before polyculture, c is a regression coefficient, f_iFor a full synergic effect, e_ijRepresenting random residual effects;

this model is represented in matrix form as: y ═ Xb + Zu + e (2);

wherein y is a table value vector, b is a fixed effect vector, u is a random effect vector, and e is a random residual vector;

x, Z are the correlation matrices corresponding to the fixed effect vector b and the random effect vector u, respectively, and the mathematical expectation and variance of the random vectors u and e are defined as:

wherein A is the blood margin coefficient matrix of all individuals,breeding value variance-additive genetic variance for an individual; h is²Is the residual force, R is the residual error;

the system of equations of the mixed model is as follows:

k＝(1-h²)/h² (5)。

4. the breeding method of the all-male macrobrachium rosenbergii rapid-growth strain according to claim 1,

f, male parent system seed reserving and propagation expanding:

the seed reserving method comprises the following steps: after harvesting parent shrimps every year, performing inter-family selection and intra-family selection, and selecting families with high breeding value and intra-family individuals;

the propagation method adopts parent secondary propagation; the specific method comprises the following steps: selecting 8 families with leading indexes and no genetic relationship from the core population in the previous year, performing positive and negative mating on every two families respectively to construct 4 mixed families, producing 30-50 thousands of offspring seeds from each mixed family for pond-by-pond culture, and selecting high-quality male shrimps from each family for subsequent mating and breeding with 'false female' shrimps after the mixed families are bred; obtaining the high-quality all-male strain.

5. The breeding method of the rapid growing strain of Macrobrachium rosenbergii according to claim 1,

standard for high quality male shrimp: the length of the male shrimp is more than 12.5cm, the weight of the male shrimp is more than 65g, the male shrimp is strong, the clamp is orange, the abdominal limbs are intact, and the shell color is bright;

standard for good quality "false female" shrimp: the length of the male shrimp is more than 11.0cm, the weight of the male shrimp is more than 45g, the male shrimp is strong, the clamp is orange, the abdominal limbs are intact, the shell color is bright, and no egg-carrying cavity exists.

Technical Field

The invention relates to the field of agricultural aquatic products, in particular to a breeding method of a rapid growth strain of all-male macrobrachium rosenbergii.

Background

Macrobrachium rosenbergii (Macrobrachium roenbergii) is a macrobrachium species in the family brachycapodaceae and is an important freshwater shrimp breeding species in the southeast region of China. The meat is delicious, tender and rich in nutrition, can be brought into the market, is more and more popular with people in China, and keeps good market demand and stable sale price. The male and female sex characteristics of the macrobrachium rosenbergii are remarkably different, the male shrimp is large in size and fast in growth, the macrobrachium rosenbergii has growth advantages, and the macrobrachium rosenbergii is popular in south China in the market. Therefore, the all-male macrobrachium rosenbergii culture has wide market prospect; although Israel has begun to utilize RNAi technology to interfere with IAG gene to produce "false female" macrobrachium rosenbergii and all-male strains produced thereby, the production cost of offspring seed is high, which limits the popularization of the offspring seed in China market; and the existing full-male macrobrachium rosenbergii seed production system does not relate to the breeding components, the offspring inbreeding coefficient is high, and the germplasm degeneration phenomena such as the reduction of the growth individuals, the reduction of the growth speed, the poor stress resistance and the like are easy to occur. Based on the establishment of the seed production technology of the all-male macrobrachium rosenbergii in the earlier stage. The existing all-male macrobrachium rosenbergii breeding technology needs to be further improved, and a new all-male macrobrachium rosenbergii strain (species) with high growth speed and strong adaptability is cultured to meet the market demand.

Disclosure of Invention

In order to solve the defects of the prior art, the invention provides a family breeding method of the all-male macrobrachium rosenbergii, and the improved all-male macrobrachium rosenbergii obtained by the breeding method has the characteristics of quick growth, uniform specification and high proportion of orange pliers, and is suitable for breeding low-density and large-size commercial macrobrachium rosenbergii.

In order to achieve the above object, the present invention adopts the following technical solutions:

a method for breeding a rapid growth strain of all-male macrobrachium rosenbergii comprises the following steps:

step one, obtaining a female parent of an all-male strain:

taking Mycoplasma maindroni as a basic population, interfering a DMRT1 gene by combining RNAi to prepare 'false female' shrimp male ZZ, mating and breeding the 'false female' shrimp and normal male shrimp to generate full male offspring, and establishing a full male Macrobrachium rosenbergii strain; preparing the 'false female' shrimp every year as a subsequent female parent;

step two, the systematic breeding of male parent parents:

a, preparing a source of a basic breeding group:

collecting high-quality macrobrachium rosenbergii resources on the basis of macrobrachium rosenbergii germplasm resource general survey, identification and genetic rule analysis in a macrobrachium rosenbergii main production area, and establishing a macrobrachium rosenbergii breeding heart group by taking macrobrachium rosenbergii No. 2 in the south Taihu lake as a basic group;

b, constructing a family:

adopting directional mating of unbalanced nest type design to construct a full/half-sib family, and taking each female shrimp as a family after spawning;

when each family is independently standardized and cultured to 3cm, performing pond-sharing growth and survival test after each family is marked with VIE;

when the individual grows to be a shrimp and reaches the market specification, measuring the weight value of the individual, counting the survival rate of each family and measuring growth data;

c, data sorting and data analysis:

carrying out preliminary statistical analysis on the obtained character data, and calculating the average value, standard deviation and variation coefficient parameters of the characters;

d, using a mixture model, estimating by a BLUP method in combination with a REML method:

calculating genetic progress aiming at single sex, estimating selection reaction of a good group and a control group by adopting a general linear model for growth data, and calculating to realize genetic progress;

e, designing a next generation mating scheme:

the principles for establishing a mating scheme comprise:

the inbreeding coefficient of the genetic relationship between the individuals to be matched is less than 0.01;

selecting individuals with high breeding value in families according to the breeding value of the individuals, and randomly matching the families;

the number of individuals selected for each family; sex ratio of male and female, the male and female ratio is 4: 1;

f, male parent system seed reserving and propagation expanding:

the seed reserving method comprises the following steps: after harvesting parent shrimps every year, performing inter-family selection and intra-family selection, and selecting families with high breeding value and intra-family individuals; the propagation method comprises the following steps: performing secondary propagation on the parent strain;

step three, expanding propagation of high-quality all-male macrobrachium rosenbergii:

selecting high-quality female shrimps from the 'false female' macrobrachium rosenbergii as female parents, and selecting high-quality male shrimps from the second-stage expanded population of the BLUP population as female parents; the two are naturally mated and propagated, thereby obtaining the high-quality all-male strain.

The breeding method of the rapid growth strain of the all-male macrobrachium rosenbergii,

b, constructing a family:

adopting directional mating of unbalanced nest type design to construct a full/half-sib family, and taking each female shrimp as a family after spawning;

when each family is independently standardized and cultured to 3cm, performing pond-sharing growth and survival test after each family is marked with VIE;

when the individual grows to be a shrimp and reaches the market specification, measuring the weight value of the individual, counting the survival rate of each family and measuring growth data;

comprehensively recording the production performance, body length, body weight, sex, mating species and genealogy of all individuals of the core group macrobrachium rosenbergii to form complete data information and realize genetic evaluation and inbreeding analysis of the macrobrachium rosenbergii;

the number of individuals tested each year is not less than 10000, and assuming that the male-female ratio of each family is 1:1, the seed reserving rate of female and male shrimps of each generation is 4% and 2% respectively.

The breeding method of the rapid growth strain of the all-male macrobrachium rosenbergii,

d, using a mixture model, estimating by a BLUP method in combination with a REML method:

calculating genetic progress aiming at single sex, estimating selection reaction of a good group and a control group by adopting a general linear model for growth data, and calculating to realize genetic progress;

wherein the genetic evaluation animal model of the single character has the formula：y_ij＝u+a_i+S_i+SP_j+cx_ij+f_j+e_ij(1)；

Wherein, y_ijDenotes the measurement of growth performance of the i-th individual, u denotes the overall mean, a_iRepresents the ith individual sports value, S_iIndicating the stationary Effect of the ith sex, SP_jShows the fixing effect of whether the female shrimp individual is oviferous or not in the pond, x_ijRepresenting the age of the family before polyculture, c is a regression coefficient, f_iFor a full synergic effect, e_ijRepresenting random residual effects;

this model is represented in matrix form as: y ═ Xb + Zu + e (2);

wherein y is a table value vector, b is a fixed effect vector, u is a random effect vector, and e is a random residual vector;

x, Z are the correlation matrices corresponding to the fixed effect vector b and the random effect vector u, respectively, and the mathematical expectation and variance of the random vectors u and e are defined as:

wherein A is the blood margin coefficient matrix of all individuals,breeding value variance-additive genetic variance-for an individual; h is²Is the residual force, R is the residual error;

the system of equations of the mixed model is as follows:

k＝(1-h²)/h² (5)。

the breeding method of the rapid growth strain of the all-male macrobrachium rosenbergii,

f, male parent system seed reserving and propagation expanding:

the seed reserving method comprises the following steps: after harvesting parent shrimps every year, performing inter-family selection and intra-family selection, and selecting families with high breeding value and intra-family individuals;

the propagation method adopts parent secondary propagation; the specific method comprises the following steps: selecting 8 families with leading indexes and no genetic relationship from the core population in the previous year, performing positive and negative mating on every two families respectively to construct 4 mixed families, producing 30-50 thousands of offspring seeds from each mixed family for pond-by-pond culture, and selecting high-quality male shrimps from each family for subsequent mating and breeding with 'false female' shrimps after the mixed families are bred; obtaining the high-quality all-male strain.

The breeding method of the rapid growth strain of the macrobrachium rosenbergii,

standard for high quality male shrimp: the length of the male shrimp is more than 12.5cm, the weight of the male shrimp is more than 65g, the male shrimp is strong, the clamp is orange, the abdominal limbs are intact, and the shell color is bright;

standard for good quality "false female" shrimp: the length of the male shrimp is more than 11.0cm, the weight of the male shrimp is more than 45g, the male shrimp is strong, the clamp is orange, the abdominal limbs are intact, the shell color is bright, and no egg-carrying cavity exists.

The invention has the advantages that:

the macrobrachium rosenbergii fast-growing strain established by the method is low in inbreeding coefficient and high in quality of germplasm after passage by breeding, analyzing data, establishing a model and designing a mating scheme;

the method comprehensively records the production performance, body length, weight, sex, mating, pedigree and the like of all individuals of the core group of the macrobrachium rosenbergii, forms complete data information, and realizes genetic evaluation and inbreeding analysis of the macrobrachium rosenbergii;

the invention carries out performance measurement on the bred macrobrachium rosenbergii, and further breeding of the population is developed by applying a BLUP breeding value selection method to improve the performance and the stable inheritance;

the male proportion of the full-male macrobrachium rosenbergii is high, the growth speed is high, the macrobrachium rosenbergii can be sold in the market early, and the yield is high;

by adopting the method for breeding the fast-growing strains, the new strains which grow fast and have uniform specification can be bred, the new strains are bright in color, high in orange pliers proportion and high in survival rate, and are suitable for low-density culture and mixed culture with other fishes and crustaceans.

Drawings

FIG. 1 is a flow chart of the first generation of artificial female shrimp production of the present invention.

Detailed Description

The invention is described in detail below with reference to the figures and the embodiments.

A method for breeding a rapid growth strain of all-male macrobrachium rosenbergii comprises the following steps:

step one, establishing an all-male strain;

the method for establishing the all-male strain comprises the following steps: the macrobrachium porterii of Burma line is taken as a basic population, RNAi is combined to interfere DMRT1 gene to prepare 'false female' macrobrachium porterii, the 'false female' macrobrachium porterii is used for mating with normal male macrobrachium porterii to breed, full male offspring is generated, and a full male macrobrachium porterii strain is established. Selecting male shrimp fries from the selected male shrimp fries every year, transfecting healthy male young shrimps (P <60) by using siRNA targeting DMRT1, and screening a pseudo-female ZZ individual with a female phenotype; as a subsequent female parent, after the full-male strain is mature, strictly and independently preserving the strain. The specific contents are disclosed in the patent of the prior application, and the application numbers are as follows: 2020101790934, respectively; the patent names are: a method for producing seeds of full-male macrobrachium rosenbergii, which is not described herein again.

Step two, performing systematic breeding on male parent parents;

1, origin of basic breeding group

Collecting high-quality macrobrachium rosenbergii resources in a macrobrachium rosenbergii main production area on the basis of developing macrobrachium rosenbergii germplasm resource general survey, identification and genetic rule analysis, and combining a southern Taihu No. 2 basic population to establish a macrobrachium rosenbergii breeding heart population;

2, constructing a family;

directional mating by adopting unbalanced nest design, pairing each male shrimp with 3 to 4 female shrimps, and establishing more than 150 full-sib families and 50 father half-sib groups in each generation;

constructing a full/half-sib family, taking each female shrimp as a family after spawning, independently hatching fertilized eggs, performing first standardized operation after hatching for two days, selecting about 2000 nauplius larvae to continue to breed into young shrimps, and then randomly selecting about 400 young shrimps to continue to breed;

when the body length of the macrobrachium rosenbergii reaches 3cm, 120 individuals are randomly selected from each family, and a visual embedded rubber mark is injected at the sixth abdominal segment; after the VIE marking of the family individual is finished, putting the family individual into 2 growth test pools respectively for growth and survival test;

when the individual grows to become a shrimp and reaches the market specification, the weight value of the individual is measured, the survival rate of each family is counted, and the growth data are measured. The growth data included: body length, skull and breastplate length, body weight, sex, marking color of each shrimp, recording measurement time and corresponding measurement indexes. The number of individuals tested each year is not less than 10000, and assuming that the male-female ratio of each family is 1:1, the seed reserving rate of female and male shrimps of each generation is 4% and 2% respectively.

3, sorting data and performing preliminary analysis;

checking each data value by comparing with the original record, after removing obvious unreasonable data, carrying out preliminary statistical analysis on the obtained character data, and calculating parameters such as the average value, the standard deviation, the variation coefficient and the like of the character;

4, estimating by using a mixture model through a BLUP method and a REML method;

the genetic evaluation animal model of the single character has the following formula: y is_ij＝u+a_i+S_i+SP_j+cx_ij+f_j+e_ij(1)；

Wherein, y_ijDenotes the measurement of growth performance of the i-th individual, u denotes the overall mean, a_iRepresents the ith individual sports value, S_iIndicating the stationary Effect of the ith sex, SP_jShows the fixing effect of whether the female shrimp individual is oviferous or not in the pond, x_ijRepresenting the age of the family before polyculture, c is a regression coefficient, f_iFor a full synergic effect, e_ijRepresenting random residual effects;

this model is represented in matrix form as: y ═ Xb + Zu + e (2);

wherein y is a table value vector, b is a fixed effect vector, u is a random effect vector, and e is a random residual vector;

x, Z are the correlation matrices corresponding to the fixed effect vector b and the random effect vector u, respectively, and the mathematical expectation and variance of the random vectors u and e are defined as:

wherein A is the blood margin coefficient matrix of all individuals,breeding value variance (additive genetic variance) for an individual; h is²Is the heritability, and R is the residual error.

The system of equations of the mixed model is as follows:

k＝(1-h²)/h² (5)；

genetic progress prediction and calculation;

the genetic progression is calculated for a single sex, the basic formula for predicting genetic progression is: Δ G ═ ip σ_A(6)；

Wherein i is the selection intensity, p is the precision of the breeding value estimation, σ_AAdditive genetic standard deviation of traits such as body weight and the like;

for both sexes, the formula is calculated as:

in the formula i_m，i_fThe selection intensity, p, of male and female shrimps, respectively_m，p_fAccuracy, sigma, of the values of the male and female shrimps_AAdditive genetic standard deviation of body weight and other characters;

for data such as growth and the like, estimating the selective reaction of the good group and the control group by adopting a general linear model, and calculating to realize genetic progress;

the general estimation model is: y is_ijk＝μ+ST_i+P_j+bA_k(ST_i)+e_ijk(8)；

Wherein, Y_ijkThe measured value of the properties such as body weight and the like; μ is the population mean; ST (ST)_iA gender pond fixation effect; p_jFor the group fixation effect, the group comprises: a good group and a control group; b is a regression coefficient; a. the_kFor average body weight before pool entry, in ST_iInternally used as covariates; e.g. of the type_ijkIs a random residual.

5, designing a next generation mating scheme;

the principles for establishing a mating scheme comprise: the inbreeding coefficient of the genetic relationship between the individuals to be matched is less than 0.01; selecting individuals with high breeding value in families according to the breeding value of the individuals, and randomly matching the families; the number of individuals selected for each family; sex ratio of male and female, the male and female ratio is 4: 1; finishing the work of formulating the mating scheme of the macrobrachium rosenbergii.

6, passage;

the seed reserving method comprises the following steps: after the harvest of the parent shrimps every year, the first seed reservation is to select between families; selecting families with the average value above according to the average breeding value of the families; the second seed reservation is the selection in the family, 2 female individuals and 1 male individual are selected from each family according to the breeding value of the individual; for the first seed reserving, the number of families is halved; the number of families of the individuals reserved for the second time can be doubled; or the total number in the breeding center is controlled to be more than 200 by adjusting the number of families reserved for the first time, and the number of families can be kept to be more than 500 in a conditional place.

Evaluating the breeding value of the offspring of the established 2-3 generation basic group, ranking according to the size sequence of the breeding value, selecting female shrimps with the breeding value ranked first 400, selecting male shrimps with the breeding value ranked first 100 from the core group, and establishing 2 generations of the macrobrachium rosenbergii core group by taking the male shrimps as units;

repeating the steps 4-5 to build the core groups of the 3 generations and 4 generations of the all-male macrobrachium rosenbergii, wherein the building coefficient of each generation is more than 200, and the measured number of the macrobrachium rosenbergii is more than 10000.

7, propagation expanding;

the propagation method comprises the following steps: selecting 8 families with leading indexes and no genetic relationship from the core population in the previous year, carrying out positive and negative mating on every two families respectively to construct 4 mixed families, producing 30-50 thousands of offspring seeds from each mixed family to carry out pond-by-pond culture, and selecting high-quality male shrimps from each family to be used for mating and breeding with subsequent female shrimps after the mixed families are bred.

Standard for high quality male shrimp: the length of the male shrimp is more than 12.5cm, the weight of the male shrimp is more than 65g, the male shrimp is strong, the clamp is orange, the abdominal limbs are intact, and the shell color is bright.

Standard for good quality "false female" shrimp: the length of the male shrimp is more than 11.0cm, the weight of the male shrimp is more than 45g, the male shrimp is strong, the clamp is orange, the abdominal limbs are intact, the shell color is bright, and no egg-carrying cavity exists.

Step three, expanding propagation of high-quality all-male macrobrachium rosenbergii;

selecting high-quality female shrimp from the pseudofemale giant freshwater prawn as a female parent, and selecting high-quality male as the female parent from the second-level expanding population of the BLUP population. The two are naturally mated and propagated, thereby obtaining the high-quality all-male strain.

The technical effect of the invention is verified by the following method for completely demonstrating the breeding of the rapid growth strain of the full-male macrobrachium rosenbergii;

establishing a pure-line macrobrachium rosenbergii pseudo female shrimp germplasm library, and breeding the full-male macrobrachium rosenbergii by combining a BLUP breeding method; the breeding flow chart of the all-male macrobrachium rosenbergii is shown in figure 1.

According to the method for breeding the new all-male macrobrachium rosenbergii strain, disclosed by the invention, from 6 months in 2019 to the present, the scale of the core group of the bred new strain of macrobrachium rosenbergii is more than 220, and high-quality all-male macrobrachium rosenbergii can be stably bred every year.

The advantages of the main technical indexes are compared as follows:

performance of	New strain	Original variety
			Blue long arm type	12.2％	14.8％
Orange long arm type	78.3％	69.3％
			Small individual	9.5％	15.9％
Average body weight (g)	45.58±5.36g	38.58±6.89g

By adopting the method for breeding the rapidly growing strains, the new strains which grow rapidly, have high sex reversion efficiency and uniform specification can be cultured, the characteristic of high proportion of orange pliers is stably appeared, and the proportion of the small-individual full-male macrobrachium rosenbergii in the new strains is 9.5 percent and is better than 15.9 percent of the original strains. Therefore, the new line has significantly better growth than the original line.

The effect of the full-male macrobrachium rosenbergii cultivation is compared with that of the common macrobrachium rosenbergii:

the all-male single-sex shrimp seeds can be used for pond culture of macrobrachium. And (3) selecting the desalinated healthy full male seedlings from the seedling raising base of the macrobrachium rosenbergii, lofting the seedlings in 6 pond water areas per mu for culture, and stocking according to the seedling stocking density of 1 ten thousand seedlings per mu. The control group is common commercial seedlings, and the breeding process refers to a common external pond breeding technology to develop a breeding test of 5 months. The experimental results are shown in the table, and the growth of the full-male macrobrachium rosenbergii in three, four and five months shows differences. When the pond is finally harvested, the average male ratio is 98.9 +/-0.4%, the average weight range is 63.1 +/-22.1 g, the feed coefficient is 1.40 +/-0.06, and the survival rate is 51.5 +/-8.2%; the male ratio of the control group is 58.6 + -4.6%, the average body weight is 47.4 + -19.6 g, the feed coefficient is 1.62 + -0.05, and the survival rate is 58.6 + -4.6. Compared with the prior art, the full-male macrobrachium rosenbergii has higher culture economic benefit under the condition of low density.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.