阅读说明：本技术 白藜芦醇作为饲料添加剂用于改善母猪乳成分的应用 (Application of resveratrol as feed additive for improving milk components of sows ) 是由 单安山 曹春宇 孙世帅 孟庆维 于 2021-01-22 设计创作，主要内容包括：本发明公开一种白藜芦醇作为饲料添加剂用于改善母猪乳成分的应用,在母猪妊娠期和哺乳期每公斤饲料中含有白黎芦醇300毫克。本发明解决了母猪乳中乳糖和脂肪含量较少的问题,本发明提高了母猪乳中乳糖和脂肪的含量,与对照组相比,初乳中乳糖含量提高了31.36％,并且常乳中的脂肪含量也提高了26.67％。并且母体补充白藜芦醇还调节了断奶仔猪的脂质代谢。(The invention discloses an application of resveratrol as a feed additive for improving milk components of sows, wherein 300mg of resveratrol is contained in each kilogram of feed of sows in the gestation period and the lactation period. The invention solves the problem of low content of lactose and fat in sow milk, improves the content of lactose and fat in the sow milk, and compared with a control group, the content of lactose in colostrum is improved by 31.36 percent, and the fat content in normal milk is also improved by 26.67 percent. And the maternal resveratrol supplementation also regulates the lipid metabolism of weaned piglets.)

1. Application of resveratrol as feed additive for improving milk component of sow is provided.

2. Use according to claim 1, characterized in that: 300mg of resveratrol is contained in every kilogram of feed during the pregnancy or lactation period of the sow.

3. The gestation period feed for improving the milk component of the sows is characterized by comprising the following components in percentage by weight: 65.970% of corn, 16.000% of wheat bran, 15.000% of soybean meal, 1.100% of stone powder, 1.000% of calcium hydrophosphate, 0.400% of salt, 0.350% of microelement premix, 0.150% of vitamin premix and 0.03% of resveratrol.

4. The lactation feed for improving the milk components of sows is characterized by comprising the following components in percentage by weight: 62.770% of corn, 4.000% of wheat bran, 24.000% of soybean meal, 3.000% of fish oil, 3.000% of soybean oil, 0.800% of stone powder, 1.300% of calcium hydrophosphate, 0.200% of lysine, 0.400% of salt, 0.350% of trace element premix, 0.150% of vitamin premix and 0.03% of resveratrol.

5. The feed according to claim 3 or 4, wherein the effective resveratrol content is greater than or equal to 96%.

6. The feed as claimed in claim 3 or 4, wherein the corn contains crude protein in an amount of 8.00% or more by weight.

7. The feed according to claim 3 or 4, wherein the trace element premix comprises 20mg of copper, 101mg of zinc, 0.2mg of selenium, 25mg of manganese, 80mg of iron and 0.3mg of iodine per kg of feed.

8. The feed of claim 3 or 4, wherein the vitamin premix comprises 8000IU of vitamin A, 32000 IU, 50IU of vitamin E, 1.5mg of vitamin K, 27.0 mg of vitamin B, 15mg of pantothenic acid, 20mg of nicotinic acid, 120.3 mg of vitamin B, 3.5mg of folic acid, 61.5 mg of vitamin B, 0.4mg of biotin and 11.6 mg of vitamin B per kilogram of feed.

Technical Field

The invention particularly relates to application of resveratrol serving as a feed additive for improving milk components of sows.

Background

Resveratrol is a polyphenol compound and mainly comes from plants such as peanut, grape (red wine), giant knotweed and the like. Resveratrol is a natural polyphenol substance with strong biological property, is also called as resveratrol, is a chemopreventive agent for tumors, and is a chemopreventive agent for reducing platelet aggregation and preventing and treating atherosclerosis and cardiovascular and cerebrovascular diseases. The immunity of the suckling piglets is reduced due to the fact that the milk sugar in the colostrums of the sows is low, and the growth and development of the suckling piglets are influenced due to the fact that the fat content is low.

Disclosure of Invention

Based on the defects, the invention aims to provide the application of the resveratrol as the feed additive for improving the milk components of the sows, and the resveratrol is used for increasing the content of lactose and fat in the milk of the sows.

The technical scheme adopted by the invention is as follows: application of resveratrol as feed additive for improving milk component of sow is provided.

Further provides the application as above, wherein the feed contains 300mg of resveratrol in every kilogram of feed in the pregnancy and lactation period of the sow.

The invention also aims to provide a gestation period feed for improving the milk component of sows, which comprises the following components in percentage by weight: 65.970% of corn, 16.000% of wheat bran, 15.000% of soybean meal, 1.100% of stone powder, 1.000% of calcium hydrophosphate, 0.400% of salt, 0.350% of microelement premix, 0.150% of vitamin premix and 0.03% of resveratrol;

the invention also aims to provide a lactation feed for improving the milk components of sows, which comprises the following components in percentage by weight: 62.770% of corn, 4.000% of wheat bran, 24.000% of soybean meal, 3.000% of fish oil, 3.000% of soybean oil, 0.800% of stone powder, 1.300% of calcium hydrophosphate, 0.200% of lysine, 0.400% of salt, 0.350% of microelement premix, 0.150% of vitamin premix and 0.03% of resveratrol.

The effective content of resveratrol in the feed is more than or equal to 96%, and the impurities in the resveratrol require that the Arabic gum is more than or equal to 90%.

The corn in the feed contains crude protein more than or equal to 8.00 percent according to weight percentage.

The further microelement premix in the feed contains 20mg of copper, 101mg of zinc, 0.2mg of selenium, 25mg of manganese, 80mg of iron and 0.3mg of iodine per kilogram of feed.

The vitamin premix in the feed further comprises 8000IU of vitamin A, 32000 IU of vitamin D, 50IU of vitamin E, K1.5mg of vitamin, 27.0mg of vitamin B, 15mg of pantothenic acid, 20mg of nicotinic acid, 120.3mg of vitamin B, 3.5mg of folic acid, 61.5mg of vitamin B, 0.4mg of biotin and 11.6 mg of vitamin B per kilogram of feed.

The invention has the advantages and beneficial effects that: the invention solves the problem of low content of lactose and fat in sow milk, improves the content of lactose and fat in sow milk, and compared with a control group, the content of lactose in colostrum is improved by 31.36 percent, and the fat content in normal milk is also improved by 26.67 percent. And the maternal resveratrol supplementation also regulates the lipid metabolism of weaned piglets. The invention provides a certain theoretical basis and technical guidance for the efficient utilization of resveratrol in the feed formulas in pregnancy and lactation.

Detailed Description

The invention is further illustrated by the following examples.

Example 1:

1. test feed

The experimental basic daily ration is prepared by referring to the pig feeding standard issued by the ministry of agriculture in 2004 in China for the nutritional needs of NRC (2012) pregnant sows and NRC (2012) lactating sows in the United states, and Chinese feed ingredient and nutritional value table (20 th edition in 2009), wherein the composition and the main nutritional level of the daily ration in the gestation period are shown in table 1, and the composition and the main nutritional level of the daily ration in the lactation period are shown in table 2. The test group added 300mg/kg resveratrol instead of corn on a basal diet basis.

TABLE 1 composition and nutritional level of basal ration during gestation (air-dry basis)

Note:¹the premix can be a daily ration grape per kilogramSupplying: 20mg of copper; 101mg of zinc; 0.2mg selenium; 25mg of manganese; 80mg of iron; 0.3mg iodine; 8000IU of vitamin A; vitamin D₃2000 IU; vitamin E50 IU; vitamin K1.5mg; vitamin B₂7.0 mg; 15mg of pantothenic acid; 20mg of nicotinic acid; vitamin B₁₂0.3 mg; 3.5mg of folic acid; vitamin B₆1.5 mg; biotin 0.4 mg; vitamin B₁ 1.6mg；

²The nutrient level is calculated value

TABLE 2 lactation basal ration composition and nutritional level (air-dried basis)

Note:¹the premix can be provided for each kilogram of daily ration: 20mg of copper; 101mg of zinc; 0.2mg selenium; 25mg of manganese; 80mg of iron; 0.3mg iodine; 8000IU of vitamin A; vitamin D₃2000 IU; vitamin E50 IU; vitamin K1.5mg; vitamin B₂7.0 mg; 15mg of pantothenic acid; 20mg of nicotinic acid; vitamin B₁₂0.3 mg; 3.5mg of folic acid; vitamin B₆1.5 mg; biotin 0.4 mg; vitamin B₁ 1.6mg；

²The nutrient level is calculated value

2. Design of experiments

The research adopts a single-factor test design, a control group is fed with basic diet, 300mg/kg of resveratrol (obtained from Nanjing Jingjing bamboo Biotechnology Co., Ltd., alcohol extraction method, liquid chromatography analysis purity of 96%) is added into the basic diet of the test group, and the influence of the addition of the resveratrol into the sow diet on the milk components and the offspring lipid metabolism is researched. The experimental selection is similar in weight, 40 sows in long times large pregnancies are born by menstruation (three or four fetuses), artificial insemination mode is adopted for breeding (Duroc boar semen), 2 control groups and experimental groups are randomly divided according to weight and number of fetuses, each group has 20 repetitions, and each repetition has 1 pig. The trial ranged from 7 days prior to sow mating to parturition.

3. Feeding management

Before the experiment, the pigsty is thoroughly disinfected and cleaned with a trough, and the pigsty is ventilated by adopting natural ventilation auxiliary machinery. 3-5 sows are raised in ground during gestation, and fed in sections during gestation period, and water is freely drunk. The feed is required to be accurately and quickly put in each time so as to reduce stress, prevent flowing and protect fetus, and does not feed moldy and deteriorated feed. Under normal conditions, the pregnancy can be determined for sows that are no longer in heat about 21 days after mating. The sow is transferred to a completely disinfected farrowing house in 110 days of gestation period and is fed by a farrowing limit fence, and the area of the farrowing fence is 1.5 multiplied by 2.1 m. The fence is internally provided with a piglet incubator, an infrared lamp is used for heating, and sows and piglets adopt duckbilled drinkers to freely drink water. In lactation period, feeding 2.0kg on the day of parturition, and adding 1.0kg daily after parturition until free feeding. After delivery, the piglets are intercropped in the same group, and 9-13 piglets are born by each sow. The iron and selenium can be supplemented by injection in 3 days after the birth of the piglet. The piglets are inoculated with various vaccines according to the immunization program of a pig farm, the piglets start to be supplemented in 7 days after birth, the cleanness and sanitation in a house are kept, the heat preservation measures of the newborn piglets are strengthened, and the piglets are weaned at the age of 21 days. And transferring the weaned sows to a breeding house, and observing oestrus.

The weight percentage of the formula in the gestation period is as follows: 65.970% of corn, 16.000% of wheat bran, 15.000% of soybean meal, 1.100% of stone powder, 1.000% of calcium hydrophosphate, 0.400% of salt, 0.350% of microelement premix, 0.150% of vitamin premix and 0.03% of resveratrol;

the weight percentage of the formula in the lactation period is as follows: 62.770% of corn, 4.000% of wheat bran, 24.000% of soybean meal, 3.000% of fish oil, 3.000% of soybean oil, 0.800% of stone powder, 1.300% of calcium hydrophosphate, 0.200% of lysine, 0.400% of salt, 0.350% of microelement premix, 0.150% of vitamin premix and 0.03% of resveratrol.

4. Data statistics

The data statistics were tested using SPSS18.0 software independent sample t, and the results were expressed as mean ± sem.

5. Example results

TABLE 3 influence of diet addition of resveratrol on sow milk composition

Note: the different lower case letters of the shoulder marks of the same row indicate that the difference is significant (P <0.05), and the same letters without shoulder marks or labels indicate that the difference is not significant (P > 0.05).

As can be seen from table 3, the addition of resveratrol to the diet significantly increased the lactose content in the colostrum, and the total solids and fat content in the normal milk ingredients were significantly increased compared to the control group. The results show that the feed of the test group has the function of improving the milk components of the sows, the mechanism of increasing the content of the lactose is that the resveratrol can improve the glucose intake in mammary cells, and the function of increasing the content of the fat is that the resveratrol plays a role in energy metabolism.

TABLE 4 influence of resveratrol in sow diets during gestation and lactation on the activity of adipose tissue enzymes in weaned piglets

Note: the different lower case letters of the shoulder marks of the same row indicate that the difference is significant (P <0.05), and the same letters without shoulder marks or labels indicate that the difference is not significant (P > 0.05).

As can be seen from Table 4, the maternal supplementation of resveratrol to regulate the lipid metabolism of weaned piglets is embodied by the increase of the activity of liposynthase (lipoprotein lipase, acetyl coenzyme A carboxylase) and the activity of lipolytic enzyme (hormone sensitive lipase), which indicates that the maternal supplementation of resveratrol not only can improve milk components, but also can influence the energy metabolism of offspring.