阅读说明：本技术 一种养殖池塘生物肥水膏的制备方法和应用 (Preparation method and application of biological fertilizer water paste for aquaculture pond ) 是由 江文涛 马家好 马加军 于 2021-01-25 设计创作，主要内容包括：本发明公开了一种养殖池塘生物肥水膏的制备方法和应用,包括下述步骤：1.酶解：取粗蛋白酶制剂1％～10％、豆粕20％～40％、水60％～80％装入酶解罐中；2.厌氧发酵酸解：按1％～10％接种量接种乳酸菌种子液,静置培养。与现有技术相比,本发明技术方案的有益效果是：本发明通过固体纯培养技术生产粗蛋白酶,然后使用生产的粗蛋白酶将豆粕进行酶解,将不溶的大分子蛋白质酶解为水体动植物可直接吸收利用的可溶性蛋白质、小肽和氨基酸,不仅提高了肥效,而且减少了大分子物质带来的负面问题。(The invention discloses a preparation method and application of a biological fertilizer water paste for an aquaculture pond, which comprises the following steps: 1. enzymolysis: 1-10% of crude protease preparation, 20-40% of soybean meal and 60-80% of water are taken and put into an enzymolysis tank; 2. anaerobic fermentation acidolysis: inoculating lactobacillus seed liquid according to the inoculation amount of 1-10 percent, and standing and culturing. Compared with the prior art, the technical scheme of the invention has the beneficial effects that: according to the invention, the crude protease is produced by a solid pure culture technology, then the produced crude protease is used for carrying out enzymolysis on the soybean meal, and insoluble macromolecular proteins are subjected to enzymolysis to obtain soluble proteins, small peptides and amino acids which can be directly absorbed and utilized by aquatic animals and plants, so that the fertilizer efficiency is improved, and the negative problems caused by macromolecular substances are reduced.)

1. A preparation method of a biological fertilizer water paste for an aquaculture pond is characterized by comprising the following steps:

s1, enzymolysis: 1-10% of crude protease preparation, 20-40% of soybean meal and 60-80% of water are taken and put into an enzymolysis tank for enzymolysis;

s2, anaerobic fermentation acidolysis: inoculating lactobacillus seed solution, and standing for culture.

2. The method for preparing the biofertilizer paste for the aquaculture pond according to claim 1, wherein the preparation of the crude protease preparation comprises the following steps:

s11, inoculating protease-producing strains into a culture medium, and culturing to obtain protease-producing strain activated bacteria liquid;

s12, inoculating the protease-producing strain activated bacterial liquid into a solid culture medium according to the inoculation amount of 1-10% for culture.

3. The method for preparing the biofertilizer paste for the aquaculture pond of claim 2, wherein the protease producing strain is one or more of bacillus subtilis, bacillus licheniformis, aspergillus oryzae and aspergillus niger; taking slant seeds of the strain producing protease, using an inoculating loop to pick 2-loop hyphae, inoculating the hyphae into a 500mL sterilized triangular flask filled with 100mL of a Chao's medium, and performing shake culture at 25-38 ℃ for 20-48 h to obtain the strain activating bacteria liquid producing protease.

4. The method for preparing a biofertilizer paste for an aquaculture pond according to claim 2, wherein the solid medium in step S12 comprises: 30-100 parts of bran, 0-60 parts of soybean meal, 0-10 parts of corn flour, 0-1 part of magnesium sulfate and 0-1 part of manganese sulfate; solid medium: the water content is 1: 0.8-1: 1.2.

5. The preparation method of the biofertilizer water paste for the aquaculture pond according to claim 1, wherein the lactobacillus seed liquid is obtained by inoculating lactobacillus slant onto a culture medium, and performing static culture at 20-40 ℃ for 3-7 days; the lactobacillus is one or more of lactobacillus plantarum, lactobacillus casei and lactobacillus acidophilus.

6. The preparation method of the biofertilizer water paste for the aquaculture pond according to claim 1, wherein in step S1, 1% -10% of crude protease, 10% -40% of soybean meal and 60% -90% of water are taken and put into an enzymolysis tank, and are heated to 30-60 ℃ by jacket or coil steam, stirred and enzymolyzed for 0.5-8 h, then continuously stirred and heated to 80-121 ℃ for inactivation for 10-60 min, and cooled to 30 ℃.

7. The preparation method of the biofertilizer water paste for the aquaculture pond according to claim 1, wherein in step S2, 0-20% of carbon source and 0-5% of inorganic salt are added into the cooled enzymatic hydrolysate, lactobacillus seed liquid is inoculated according to 1-10% of inoculation amount, the mixture is pumped into a culture tank by a pump, and standing culture is carried out for 3-20 days at normal temperature to obtain biofertilizer water paste; the carbon source can be one or more of glucose, molasses, starch, dextrin, corn flour, rice bran and the like, and the inorganic salt can be one or more of zinc sulfate, magnesium sulfate, ferrous sulfate, magnesium chloride, ferrous chloride, ferric ammonium citrate, calcium superphosphate, manganese chloride, copper sulfate, potassium dihydrogen phosphate and the like.

8. The preparation method of the biofertilizer water paste for the aquaculture pond as claimed in claim 1, wherein after enzymolysis, the water-soluble protein content is more than or equal to 15.0%, and the acid-soluble protein content is more than or equal to 10.0%.

9. The method for preparing a biofertilizer paste for an aquaculture pond according to claim 1, wherein the number of lactic acid bacteria after anaerobic fermentation is at least 2.0 x 10⁹CFU/g。

10. Use of a biofertilizer paste prepared by the method according to any one of claims 1-9 in the aquaculture field or for reducing nitrite in water.

Technical Field

The invention relates to the field of aquaculture, in particular to a preparation method and application of a biological fertilizer water paste for an aquaculture pond.

Background

The fish culture is carried out with water first, and the water fertilization is an important link in the culture process. The fertilizer is applied to fertilize water, so that not only can bait organisms be cultivated to provide baits for fishes and shrimps, but also certain organic fertilizers can be directly eaten by the fishes. Therefore, the rich water is widely applied to aquaculture. The common fertilizers used in aquaculture at present comprise commercial fertilizers, inorganic fertilizers, organic fertilizers and the like. If the organic fertilizer is treated improperly, the water body is easy to lack of oxygen, and harmful substances such as hydrogen sulfide, ammonia and the like are generated, so that the fishes and the shrimps are ill; the inorganic fertilizer has quick effect but short duration, and is easy to cause bottom odor and soil hardening; the commercial fertilizer has uneven quality and relatively high price.

Disclosure of Invention

The present invention is directed to overcoming at least one of the disadvantages in the prior art set forth above.

In order to solve the technical problems, the invention mainly aims to provide a preparation method of a biological fertilizer water paste for an aquaculture pond, which comprises the following steps:

s1, enzymolysis: 1-10% of crude protease preparation, 20-40% of soybean meal and 60-80% of water are taken and put into an enzymolysis tank for enzymolysis;

s2, anaerobic fermentation acidolysis: inoculating lactobacillus seed liquid according to the inoculation amount of 1-10 percent, and standing and culturing.

Preferably, the preparation of the crude protease preparation comprises the following steps:

s11, inoculating protease-producing strains into a culture medium, and culturing to obtain protease-producing strain activated bacteria liquid;

s12, inoculating the protease-producing strain activated bacterial liquid into a tray filled with a solid fermentation culture medium according to the inoculation amount of 1-10%.

Preferably, the protease-producing strain is one or more of bacillus subtilis, bacillus licheniformis, aspergillus oryzae and aspergillus niger; taking slant seeds of the strain producing protease, using an inoculating loop to pick 2-loop hyphae, inoculating the hyphae into a 500mL sterilized triangular flask filled with 100mL of a Chao's medium, and performing shake culture at 25-38 ℃ for 20-48 h to obtain the strain activating bacteria liquid producing protease.

Preferably, the solid fermentation medium formula comprises (by weight portion): 30-100 parts of bran, 0-60 parts of soybean meal, 0-10 parts of corn flour, 0-1 part of magnesium sulfate, 0-1 part of manganese sulfate and a solid culture medium: the water content is 1: 0.8-1: 1.2 (weight ratio).

Preferably, the lactobacillus seed solution is obtained by inoculating a lactobacillus slant into a culture medium and performing static culture for 3 to 7 days at the temperature of between 20 and 40 ℃; the lactobacillus is one or more of lactobacillus plantarum, lactobacillus casei and lactobacillus acidophilus.

Preferably, 1-10% of crude protease, 10-40% of soybean meal and 60-90% of water are taken and put into an enzymolysis tank, the mixture is heated to 30-60 ℃ by jacket or coil steam, stirred and enzymolyzed for 0.5-8 h, then continuously stirred and heated to 80-121 ℃ for inactivation for 10-60 min, and cooled to 30 ℃.

Preferably, in step S2, 0-20% of carbon source and 0-5% of inorganic salt are added to the cooled enzymatic hydrolysate, and lactobacillus seed liquid is inoculated according to 1-10% of inoculum size, pumped into a culture tank by a pump, and subjected to static culture at normal temperature for 3-20 days to obtain biological fertilizer water paste; the carbon source can be one or more of glucose, molasses, starch, dextrin, corn flour, rice bran and the like, and the inorganic salt can be one or more of zinc sulfate, magnesium sulfate, ferrous sulfate, magnesium chloride, ferrous chloride, ferric ammonium citrate, calcium superphosphate, manganese chloride, copper sulfate, potassium dihydrogen phosphate and the like.

Preferably, the water-soluble protein is more than or equal to 15.0 percent and the acid-soluble protein is more than or equal to 10.0 percent after enzymolysis.

Preferably, the number of lactic acid bacteria after anaerobic fermentation is at least 2.0X 10⁹CFU/g。

The invention also aims to provide application of the biological fertilizer water paste for the aquaculture pond prepared by the method in the aquaculture field or reduction of nitrite in water.

Compared with the prior art, the technical scheme of the invention has the beneficial effects that:

1. the invention uses the crude protease to carry out enzymolysis on the soybean meal, and carries out enzymolysis on insoluble macromolecular protein into soluble protein, small peptide and amino acid which can be directly absorbed and utilized by aquatic animals and plants, thereby not only improving the fertilizer efficiency, but also reducing the negative problems brought by macromolecular substances.

2. Compared with the common organic fertilizer, the biological fertilizer water paste for the aquaculture pond is added with the exogenous biological agent for promoting the decomposition of organic materials in the anaerobic fermentation process and the microbial flora with specific functions so as to improve the action effect.

Detailed Description

The present invention will be described in detail below. The specific examples are intended to illustrate the present invention in further detail, and are not intended to limit the scope of the present invention. Reagents, equipment and methods employed in the present invention are reagents, equipment and methods conventionally commercially available in the art and conventionally used methods, unless otherwise specified. Unless otherwise specified, "parts" in the present examples are in parts by weight.

A biological fertilizer water paste for an aquaculture pond and a preparation method thereof comprise the following steps:

1. preparing seeds: taking slant seeds of the protease-producing strain, picking 2-ring hyphae by using an inoculating ring, inoculating the hyphae into a 500mL sterilized triangular flask filled with 100mL of a Chao's medium, and performing shake culture at the temperature of 25-38 ℃ for 20-48 h to obtain protease-producing strain activated bacteria liquid; taking one lactic acid bacteria slant, selecting 2-ring lactic acid bacteria lawn by using an inoculating ring, inoculating the lactic acid bacteria lawn into an MRS liquid culture medium, and standing and culturing for 3-7 days at the temperature of 20-40 ℃ to obtain a lactic acid bacteria seed solution; and (4) determining culture conditions and time according to the optimal growth temperature and growth curve of the seeds. Seeds do not grow well when the time is too short, and the seeds die poorly when the time is too long. Poor or weak seeds may lead to poor acidolysis.

2. Preparation of crude protease: inoculating 1-10% of protease strain activating bacteria liquid into a shallow tray filled with a solid fermentation culture medium, and culturing for 2-5 days in a constant-temperature culture chamber at 30 ℃ to obtain crude protease; the strain producing enzyme is cultured according to the inoculation amount, the culture temperature and the culture time, the strain grows fast, the protease yield is high, and the enzyme activity is high. The enzyme activity is high, the natural enzymolysis effect is good, the produced small peptides and amino acids are more, and the fertilizer and water effect is good.

3. Enzymolysis: taking 1-10% of the crude protease, 10-40% of the soybean meal and 60-90% of water in the step (2) according to the mass ratio, putting the crude protease, the soybean meal and the water into an enzymolysis tank, heating the crude protease, the soybean meal and the water to 30-60 ℃ through jacket or coil steam, stirring and carrying out enzymolysis for 0.5-8 h, then continuously stirring and heating the crude protease to 80-121 ℃ for inactivation for 10-60 min, and cooling the crude protease, the soybean meal and the water to 30 ℃; the temperature is the optimal enzyme activity temperature of the used crude enzyme preparation, the small peptide amino acid produced by enzymolysis for 0.5-8 h is the most, the enzymolysis effect is poor with short time, and the cost is high with long time. The heating to 80-121 ℃ is to inactivate the enzyme and kill the mixed bacteria at high temperature, which is beneficial to the growth, acid production and acidolysis of the target strains. The cooling to 30 ℃ is for inoculation (the thalli are inactivated and even dead due to overhigh temperature, and the thalli are poor in activity due to overlow temperature).

4. Anaerobic fermentation acidolysis: adding 0-20% of carbon source and 0-5% of inorganic salt into the cooled enzymatic hydrolysate, inoculating lactobacillus seed liquid according to the inoculation amount of 1-10%, pumping into a culture tank by a pump, and standing and culturing for 3-20 days at normal temperature to obtain the biological fertilizer water paste.

As an improvement of the specific embodiment, the protease-producing strain can be one or a combination of bacillus subtilis, bacillus licheniformis, aspergillus oryzae and aspergillus niger, the protease-producing capability of the strain is strong, and the crude protease obtained after the strain is cultured can carry out enzymolysis on the soybean meal to obtain soluble nitrogen, small peptides and amino acids, so that the rapid fertilization is facilitated.

As an improvement of the specific embodiment, the lactic acid bacteria have the effect of degrading nitrite.

As a modification of the specific embodiment, the lactic acid bacteria can be one or more of lactobacillus plantarum, lactobacillus casei and lactobacillus acidophilus.

As an improvement of the specific embodiment, the formula of the solid fermentation medium is as follows: 30-100 parts of bran, 0-60 parts of soybean meal, 0-10 parts of corn flour, 0-1 part of magnesium sulfate, 0-1 part of manganese sulfate and a solid culture medium: the water content is 1: 0.8-1: 1.2.

As an improvement of the specific embodiment, after enzymolysis, the water-soluble protein is more than or equal to 15.0 percent, and the acid-soluble protein is more than or equal to 10.0 percent. The degree of hydrolysis is measured in these two metrics.

As a modification of the specific embodiment, the carbon source may be one or more combinations of glucose, molasses, starch, dextrin, corn flour, rice bran, etc., and the inorganic salt of step (4) may be one or more combinations of zinc sulfate, magnesium sulfate, ferrous sulfate, magnesium chloride, ferrous chloride, ferric ammonium citrate, calcium superphosphate, manganese chloride, copper sulfate, potassium dihydrogen phosphate, etc.

As an improvement of the specific embodiment, the number of the lactic acid bacteria reaches 2.0 multiplied by 10 when 3 days of anaerobic fermentation⁹CFU/g. High water-soluble substance content, and high and durable fertility.

As an improvement of the specific embodiment, after the culture is finished, the pH of the culture solution is less than or equal to 4.5, and the culture system is stable.

As an improvement of the specific embodiment, the biological fertilizer water paste also has the function of reducing nitrite in the aquaculture water.

With the development of the amino acid type innovative fertilizer technology, the product value of the novel fertilizer is increasingly improved, and good economic value and resource utilization value can be realized by using the plant protein of agricultural and sideline products as the fertilizer for production. The technology firstly adopts a pure culture technology, preferably selects a strain producing protease, preferably selects an enzyme production culture medium and enzyme production culture time, produces crude protease by a solid pure culture technology, improves the enzyme production capacity and reduces the production cost. Then, the optimized plant protein is subjected to enzymolysis by using crude protease under the optimized process conditions, finally, the trace elements are reasonably proportioned in the enzymolysis mixture, and the enzymolysis mixture is subjected to fermentation and acidolysis by using lactic acid bacteria, so that the content of soluble protein, small peptide, amino acid and other substances is improved, the fertilizer efficiency is enhanced, and the pollution is reduced.

The technology has high production efficiency, low cost and simple process, and can meet the requirement of large-scale production. After the product is applied to a water body, the product can not only fertilize water-cultured algae, but also improve water quality, improve fish immunity, reduce disease occurrence, increase culture yield, reduce fish culture cost and improve fish quality. The development and application of the product meet the requirements of scientific development and can make a certain contribution to the sustainable and healthy development of the aquaculture industry in China.

Compared with the prior art, the technical scheme of the invention has the beneficial effects that:

1. according to the invention, the crude protease is produced by a solid pure culture technology, then the produced crude protease is used for carrying out enzymolysis on the soybean meal, and insoluble macromolecular proteins are subjected to enzymolysis to obtain soluble proteins, small peptides and amino acids which can be directly absorbed and utilized by aquatic animals and plants, so that the fertilizer efficiency is improved, and the negative problems caused by macromolecular substances are reduced. The use of self-cultured crude protease is less costly than the use of commercial protease.

2. Compared with the common organic fertilizer, the biological fertilizer water paste for the aquaculture pond is added with the exogenous biological agent for promoting the decomposition of organic materials in the anaerobic fermentation process and the microbial flora with specific functions so as to improve the action effect.

3. The biological fertilizer water paste for the aquaculture pond, disclosed by the invention, has the advantages that all the used raw materials are feed-grade raw materials, so that the biological fertilizer water paste can fertilize water and can be directly taken by aquatic animals, the utilization rate is higher, no pollution is caused to a water body, and the healthy growth of the aquatic animals can be promoted. After the product is subjected to enzymolysis and anaerobic fermentation, the water-soluble protein is more than or equal to 15.0 percent, the acid-soluble protein is more than or equal to 10.0 percent, and the number of lactic acid bacteria reaches 2.0 multiplied by 10⁹CFU/g, high content of water-soluble substances, and carbon-nitrogen ratioReasonable, quick and durable fertilizer effect.

4. The biological fertilizer water paste for the culture pond can effectively control harmful algae such as basket algae, blue algae and the like, directionally culture beneficial algae such as green algae, diatom and the like, has rich quantity and variety of algae after water is fertilized, is stable in algae and can effectively prevent algae from aging.

5. According to the biological water fertilizing paste for the aquaculture pond, algae are fertilized, ammonia nitrogen and nitrite in the pond are effectively converted and reduced, the growth environment of aquatic animals is optimized, healthy growth of fishes and shrimps is promoted, and aquaculture risks are reduced.

6. The biological fertilizer water paste for the aquaculture pond is stable in pH, algal phase and bacterial phase after being fertilized with water, beautiful in water color and obvious in water stabilizing effect. The microorganisms can remove organic matters in water because the microorganisms need to utilize the organic matters for growth and propagation.

Example one

(1) Taking aspergillus oryzae slant seeds, using inoculating loop to pick 2-loop hyphae, inoculating into a 500mL sterilized triangular flask filled with 100mL Chao's culture medium, and performing shake culture at 30 ℃ for 36h to obtain aspergillus oryzae activated bacterial liquid; taking one inclined plane of lactobacillus acidophilus, selecting 2-ring lactobacillus acidophilus lawn by using an inoculating ring, inoculating into an MRS liquid culture medium, and standing and culturing at 37 ℃ for 3 days to obtain a lactobacillus seed solution; (2) inoculating 2% of Aspergillus oryzae activated bacterial liquid into a shallow tray filled with a solid fermentation culture medium, and culturing for 4 days in a constant-temperature culture chamber at 30 ℃ to obtain crude protease; wherein the solid fermentation medium comprises the following components in percentage by weight: 60 parts of bran, 35 parts of soybean meal, 5 parts of corn flour, 0.1 part of magnesium sulfate, 0.1 part of manganese sulfate and 100 parts of water; (3) 2% of crude protease, 20% of soybean meal and 78% of water are taken according to the mass ratio and are filled into an enzymolysis tank, a coil is heated to 50 ℃ by introducing steam, stirred and enzymolyzed for 1h, then continuously stirred and heated to 95 ℃ for inactivation for 50min, and cooled to 30 ℃; (4) anaerobic fermentation acidolysis: adding 2% of glucose and 1% of inorganic salt into the cooled enzymatic hydrolysate, inoculating lactobacillus seed liquid according to the inoculation amount of 5%, and standing and culturing for 7 days at normal temperature to obtain the biological fertilizer water paste. The detection shows that the water-soluble protein is 17.4 percent, the acid-soluble protein is 13.7 percent, and the viable count of the lactobacillus reaches 5.0 multiplied by 10⁹CFU/g，pH4.2。

Example two

One example of the biological fertilizer water paste for fertilizing, regulating and stabilizing water in pond for mixed culture of fishes and shrimps

A shrimp and fish mixed culture pond in the Shangshan district of Guangdong province. Mainly culturing Penaeus vannamei Boone, culturing four Chinese carps in a mixed mode, wherein the area is 43 mu, the average water depth is one meter, and 12 impeller oxygen increasing machines are arranged. Detecting water quality, ammonia nitrogen 0.5mg/L and nitrite 0.3mg/L, regularly supplementing inorganic fertilizer, and basically changing little every day. The bio-fertilizer water paste is used periodically, the bio-fertilizer water paste is used once every half month, two barrels are used every time, three barrels are used every time when the water temperature is reduced, the water color is green brown, the bio-fertilizer water paste is relatively fresh and stable, the water quality is detected, and the ammonia nitrogen is 0.1mg/L and the nitrite is 0.05 mg/L. The biological water fertilizing paste has the advantages of outstanding water fertilizing effect, stable algae, basically few blue algae, beautiful water color, refreshing and bright effect and stable water quality.

EXAMPLE III

One example of the biological fertilizer water paste for fertilizing and promoting the health of fishes and shrimps in mixed culture pond

And the other shrimp and fish polyculture pond in the Shanghai district of Guangdong province. Mainly culturing Penaeus vannamei Boone, mixedly culturing four Chinese carps, wherein the area is 60 mu, the average water depth is 1.2 m, and 14 impeller oxygen increasing machines are arranged. The water quality is slightly clear, the brightness of the water quality is reduced, the dissolved oxygen is reduced, the water color is obviously reduced, and the fish and shrimp eat slowly. The water measuring index comprises 0.4mg/L of ammonia nitrogen, 0.1mg/L of nitrite, pH8.2 and 3mg/L of dissolved oxygen. In the noon, 4 barrels of biological fertilizer water paste are used immediately, and a small amount of compound fertilizer is matched. After two days, the water quality is detected, the ammonia nitrogen is 0mg/L, the nitrous acid is 0mg/L, the pH is 8.6, and the water color is changed into fertilizer and is very fresh and cool. The microscopic algae mainly comprises large green algae and diatoms, so that the fish and shrimp eat more quickly, the whole activity of the shrimps is better, and the shrimps have more activity and glossiness. The biological fertilizer water paste has high carbon, low nitrogen, complete dissolution, no sediment and no pollution to water, can fertilize water-cultured algae, can reduce ammonia nitrogen and nitrite, and promotes the healthy growth of fishes and shrimps.

Example four

Biological fertilizer water paste matched with 'beneficial bacterium and multiple II' fertilizer water prevention and control one example of blue algae

A4 mu area of penaeus vannamei intensive culture pond with an average water depth of 1.4 m is prepared in Pinggang Zhen of Yangjiang city, Guangdong province, 1 impeller type aerator with 3kW is prepared, and the specification of the penaeus vannamei is 32 per jin. The water color of the pond is found to be dark green by the aged and the floods at 10 months and 23 am at about 10:00 am, so that the blue algae bloom is formed to float on the surface of the water body, and the ingestion activity of the prawns is poor. In the morning of 24 days in 10 months, the liquid is clear, half barrels of the biological fertilizer water paste and 3 bottles of the bacterial growth promoting agent II are poured outside, and the observation effect in the afternoon is not obvious. In the morning and sunny days of 10 months and 25 days, half barrel of the biological fertilizer water paste and 3 bottles of the bacterial growth promoting agent II are used again, and the water body is observed to become clear in the afternoon. In 26 days of 10 months, sunny days and afternoon, the water color turns yellow green, and the prawns recover the ingestion activity. The biological fertilizer water paste can provide long-acting organic carbon source and nitrogen source, and create favorable conditions for the growth of diatom and green algae, thereby effectively inhibiting the growth and propagation of blue algae in an interspecific competition mode. The main component of the "Lijunduo II" is lactic acid bacteria, which can accelerate the material circulation speed of water body, release a large amount of deposited organic carbon source in the substrate and enhance the use effect of the "biological fertilizer water paste".

EXAMPLE five

Biological fertilizer water paste for treating one slow feeding condition of prawns caused by blue algae outbreak

One 7 mu of south America white shrimp intensive culture pond in Yangjiang city of Guangdong province has the average water depth of 1.4 meters and 3 impeller type oxygen increasing machines of 1.5 kW. 22 days of 20 months in 2018, the Shangtong boss finds that a large amount of blue-green algae float on the water surface at the downwind position, the feed intake of the prawns is reduced, and the phenomena of jejunum, empty stomach and white feces occur; 10 month, 22 morning, 10:00, using biological fertilizer water paste of 7 mu/barrel, and simultaneously pumping a small amount of seawater; 10 month 23 morning 10:00, reusing the biological fertilizer water paste by 7 mu/barrel, and simultaneously pumping in a small amount of seawater; the shrimp pond is observed in the morning in 24 months in 10 months, the water quality is obviously improved, the white feces of the shrimps are reduced, and the feed is recovered to be normal. The biological fertilizer water paste is a long-acting organic fertilizer, provides nutrition for the growth and the propagation of diatom and green algae, contains a large amount of live bacteria, and inhibits the growth of the blue algae in an interspecific competition mode. Meanwhile, the addition of seawater is also beneficial to improving the water quality, controlling white feces and promoting the prawn feeding.

EXAMPLE six

Biological fertilizer water paste fertilizer water treatment ammonia nitrogen and nitrite example

One pond of 14 mu is reserved in the flat hills town of Yangjiang City, Guangdong province, the area of the pond is 14 mu, the average water depth is 1.3 m, and 2 impeller type aerators of 1.5kW and 2 waterwheel type aerators of 3.0kW are matched. After 11 months and 3 days and 10:00, the customers find that the ingestion of the prawns is slow, the pH value of the pond is 8.7, the ammonia nitrogen is 0.6mg/L and the nitrite is 0.4mg/L by using the simple water quality kit, and the biological fertilizer water paste is used for 1 barrel and 15 kg/barrel on the same day, and the whole pond is splashed. 10:00 in 5 days after 11 months, basically recovering the normal ingestion of the prawns, and measuring the physicochemical indexes of the water quality of the pond: pH value 8.4, ammonia nitrogen 0.4mg/L, nitrite 0.2 mg/L. 10:00 in 11 months, 7 days and good feeding condition of the prawns. Measuring the physicochemical indexes of the water quality of the pond: pH8.0, ammonia nitrogen 0.2mg/L and nitrite 0.1 mg/L.

The parts not involved in the present invention are the same as or can be implemented using the prior art. It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.