阅读说明：本技术 一种有机虾仁的加工方法 (Processing method of organic shrimp meat ) 是由 陈锚 孙传伟 何娟 于 2021-02-07 设计创作，主要内容包括：一种有机虾仁的加工方法,包括如下步骤：原料准备、清洗、分级挑拣、片冰保存、消毒沥水、计量装盘、速冻、镀冰衣,本发明提高了有机虾仁的加工质量和效率。(A processing method of organic shrimp meat comprises the following steps: the method comprises the steps of raw material preparation, cleaning, sorting by stages, flake ice storage, disinfection and draining, metering and tray loading, quick freezing and ice coating.)

1. The processing method of the organic shrimp meat is characterized by comprising the following steps:

(1) preparing raw materials: selecting cultured organic penaeus vannamei boone;

(2) cleaning: cleaning the organic penaeus vannamei boone with the head removed in a cleaning machine by using ice water with the temperature below 10 ℃, and cleaning the residual penaeus vannamei boone paste;

(3) sorting in a grading manner: grading according to the number range required by a client, strictly controlling the uniformity according to the sorting requirement, and picking out unqualified products;

(4) and (4) secondary inspection: placing the cleaned product on a goods inspection table for inspection, and picking out impurities of the dried small shrimps, the shrimp claws, the intestinal glands and the secondary shrimps and products which do not meet the specification requirement; then putting the qualified product into a heat-preserving barrel, and preserving the shrimp layer by layer with ice-dry ice; (5) single freezing: uniformly spreading the drained product on a plate belt, ensuring that the quick-freezing temperature is below-30 ℃, and keeping the center of the quick-frozen shrimp body at a temperature below-18 ℃;

(6) metering and dishing up: according to different specifications, products are measured by an automatic weighing machine and automatically packaged into a tray or a box, and purified ice water is added before the products enter a conveyor belt and reach a quick freezing machine, so that the shrimp bodies cannot be warped and are exposed out of the box;

(7) quick-freezing: the product is sent into a single freezer, the temperature of a liquid nitrogen instant freezer needs to continuously reach-90 ℃, the temperature of a double helix instant freezer needs to continuously reach-35 ℃, and the central temperature of the product after quick freezing reaches below-18 ℃;

(8) metering and weighing: weighing according to the customer requirements;

(9) plating ice clothes: plating the ice coating with ozone water of below 3 ℃, measuring the temperature of the ice coating water once every 1 hour, and measuring the ice coating rate once every 30 minutes;

(10) and (4) placing the organic shrimp meat subjected to metal detection into a refrigeration house for storage.

2. The processing method of organic shrimp meat according to claim 1, wherein the method for cultivating organic penaeus vannamei in step (1) comprises the following steps:

(1) modification: reconstructing an original seawater salt pan evaporation pond with water level of 60-70 cm and water level of more than one thousand mu to several thousand mu into a single culture pond;

(2) pretreatment of the pool bottom: draining water, using 50-200 kg of quicklime in each mu of seawater salt pan culture pond, mechanically ploughing for 20-30 cm, and airing for more than 30 days;

(3) early-stage water treatment: 15-20 days before putting the fries, killing wild trash fishes and other aquatic animals by using tea bran of 20 g/m 3, stirring the prepared speartail goby mincer at intervals of 3 days, soaking and fermenting by using lactic acid bacteria for 3-7 days, and using 0.5-1 kg per mu according to transparency; the wheat bran fermented by the lactic acid bacteria is used at intervals, and 2-3 kilograms of the wheat bran are used per mu until the water transparency is 40-60 cm;

(4) seedling releasing: the water temperature reaches 21-25 ℃, the standard coarse shrimp larvae are 2.0-2.5 cm, and the test of various viruses, vibrios and enterohepatic hydatid and emergency test are carried out; after water testing, the survival rate of the shrimp seedlings can reach 95% in 24 hours, and 1.2-1.5 million organic shrimp seedlings are put in each mu;

(5) feeding: feeding the fairy shrimp at 1-1.5 kg per mu twice on the seedling release day, wherein the daily increase is 5-10%, and if the water transparency is more than 60cm, the feeding amount of the fairy shrimp is reduced to half; if the water quality transparency is more than 80cm, stopping feeding the artemia cystokiniana, increasing the water change amount, and supplementing minced fillet of the lobster and tiger fish fermented by lactic acid bacteria; feeding the mixed feed for 2-3 meals every day when the shrimp body length is more than 6cm in an auxiliary mode, and adjusting the feeding quantity according to a bait observation platform;

(6) water quality regulation and control: the water quality is regulated and controlled by the cooperation of the bacillus and the wheat bran fermented by the lactic acid bacteria, and the bacillus and the wheat bran fermented by the lactic acid bacteria are used once every half month;

(7) disease prevention and control: after 15-20 days of fry release, the prawn grows for about 5cm, 4-5 cm of the lobster tail goby domesticated and consistent with the salinity of a target pond is released per mu, and when the prawn grows to about 7-8 cm, a prawn guide net is placed every 40-50 mu by utilizing the characteristics that sick shrimps and weak shrimps move along the sides of the pond and attached to the water surface; splashing 30 g of Vc raw powder in a full pool per mu of land before and after sudden change of weather; reed is planted in saline-alkali soil around the aquaculture pond, birds are attracted to lay eggs, habitat is increased, and the birds are attracted to clean diseased shrimps and weak shrimps floating on the water surface in time;

(8) natural illumination is carried out in the whole culture period;

(9) collecting shrimps in a round way, culturing for 50-60 days, using a shrimp guide net with a corresponding net mesh to start collecting the shrimps according to the requirements of customers for purchasing shrimp specifications, collecting the shrimps till the end of 10 months to the beginning of 11 months, discharging water at the temperature lower than 15 ℃, and finishing collecting the shrimps.

Technical Field

The invention relates to aquaculture, in particular to a processing method of organic shrimp meat.

Background

China is a big south America white shrimp breeding country, starts to become an important breed for breeding the shrimps in China in 1999, rapidly develops in the three provinces in south, and has become a shrimp breeding breed which is generally popularized in China and has the top yield up to now; the method has the advantages that while the production is developed at a high speed, serious problems of uneven seedling quality, virus carrying, slow growth and the like occur, and the sustainable development of the penaeus vannamei boone breeding industry in China is directly threatened; since 2017, the cultivation success rate of southern Hainan, Guangdong, Guangxi, Zhejiang, Jiangsu and other provinces is reduced sharply, and the cultivation of northern Shandong, Hebei, Tianjin and other provinces in a salt pan special ecological system in the cities of Shandong, Hebei, Tianjin and other provinces is raised regardless of cultivation area, yield, success rate and product quality; the area of the salt pan in the east coastal region of China is 505.5 ten thousand mu, which is far larger than the area of 195 ten thousand mu of the seawater culture pond in China; the salinity of the original group of the penaeus vannamei boone is 10-25 per mill optimally, and the salinity of a special ecosystem of a salt pan is only about 10 percent of 25-30 per mill; the salinity is 30-50 per mill and 50-60 percent, and the salinity is 50-65 per mill and about 30 percent; in addition, the area of the general pond of the salt pan is large and varies from hundreds of mu to thousands of mu, and the water level is only 60-70 cm; the growth period in the north is short; the southern small-area pond culture technology is difficult to copy to a special ecosystem of a salt pan; the problem of how to improve the processing yield of the shelled shrimps of the penaeus vannamei in the salt pan is the existing problem.

Disclosure of Invention

In order to solve the problems, the invention provides a processing method of organic shrimp meat, which comprises the following steps:

the introduced shrimp larvae should be organic shrimp larvae, allowing the introduction of conventional shrimp larvae when organic shrimp larvae are not available.

1. Site selection of the farm:

a) the natural water area beside the water surface of the breeding base can not be polluted. The aquatic ecology around the farm is balanced with the aquatic and terrestrial ecosystems around the farm, which is helpful to keep the biodiversity of the water area. Originally, exceed thousand mu to several thousand mu, the primary evaporation pond of water level 60~70 cm's sea water salt pan reforms transform into single 100~500 mu, water level 120~150 cm. And (3) arranging ring ditches outside 10-20 meters of the four side slopes, wherein the width of each ring ditch is 20-30 meters, and the depth of each ring ditch is 40-60 cm. Each pond can be provided with an independent drainage system (called ' not ' type) for each condition, and the upstream water outlet can be a downstream water inlet for each condition, and the water flow is S ' type.

b) The culture area is isolated from the outside by adopting a dam, a production road and a large drainage channel, and is favorable for checking the water quality production environment.

2. The cultivation water quality requirement is as follows:

the water quality must meet the requirements of GB11607 fishery water quality standard.

3. Providing bait:

the organic penaeus vannamei boone is bred, fed with the fairy shrimp (eggs) and fed with artificially mixed feed in an auxiliary manner.

4. Artificial breeding:

1) and pool bottom pretreatment: draining off water, using 50-200 kg of quicklime per mu, properly using the quicklime in low-lying places for multiple purposes, mechanically ploughing by 20-30 cm, and airing for more than 30 days.

2) Earlier stage water treatment: 15-20 days before putting seedlings, killing wild trash fishes and other aquatic animals (simultaneously having the function of fertilizing water) by using tea bran of 20 g/m 3, stirring the wild trash fishes and other aquatic animals at intervals of 3 days by using a prepared spear goby (C.stigmaias) meat mincer, soaking and fermenting the minced wild trash fishes and other aquatic animals for 3-7 days by using lactic acid bacteria, and using 0.5-1 kg per mu according to the transparency; the wheat bran fermented by the lactic acid bacteria is used at intervals, and 2-3 kilograms of the wheat bran are used per mu until the water transparency is 40-60 cm. The later stage water inlet is filtered by a 40-mesh filter bag. The soil blocking dam separates the organic culture area to prevent aquatic organisms of other culture systems from entering the organic culture farm and preying on the organic aquatic organisms, and meanwhile prevents the aquatic organisms of the organic culture farm from entering other culture water bodies.

3) And seedling releasing: the water temperature reaches 21-25 ℃, the standard coarse shrimp larvae are about 2.0-2.5 cm, and the test is carried out on various viruses, vibrios and enterocoelia entomosis and the emergency test; after water testing, the survival rate of the seedlings can be released within 95% in 24 hours, and the number of released seedlings per mu is 1.2-1.5 ten thousand.

4) Feeding: the fairy shrimp is fed on the day of seedling release, the fairy shrimp is hatched in a secondary brine pond (with the salinity of 65-95 per mill) in advance, 1-1.5 kilograms per mu is fed, and the quantity is increased by 5-10% every day in two times. If the water quality transparency is more than 60cm, the feeding amount of the fairy shrimp is reduced to a half; if the water quality transparency is more than 80cm, the artemia stop feeding, the water change amount is increased, and minced fillet of the lance tail goby (C.stigmaias) fermented by lactic acid bacteria is supplemented. And (3) starting auxiliary feeding of the compound feed when the shrimp body length is larger than 6cm, feeding 2-3 meals every day, adjusting the feeding quantity according to a bait observation platform, normally feeding on the bait observation platform, and leaving about 2% of the feeding quantity of each time on the bait observation platform to test the reasonable feeding quantity of the prawns. The sanitation indexes and the limit of the feed meet the regulations of the safety limit of organic food fishery feed (SCl052-2001) in the standard of the aquatic product industry.

5) And water quality regulation and control: the water quality can be regulated by using the wheat bran fermented by the bacillus and the lactobacillus, and the wheat bran fermented by the bacillus and the lactobacillus is used once every half month.

6) Disease prevention and control: when the shrimps grow to about 7-8 cm, by utilizing the characteristics that the sick shrimps and the weak shrimps move along the sides of the pool and attached to the water surface, a modified shrimp guide net is placed every 40-50 mu, from the side slope, a 30-50 m 60-mesh net is pulled from 30-50 cm underwater to 5-10 cm above the water surface, the guide net is arranged at one end far away from the side slope, the sick shrimps and the weak shrimps are induced into hubs, and the shrimps are cleaned every 2-3 days, so that the sick shrimps and the weak shrimps are prevented from being eaten by healthy shrimps and the infection source is cut off. 30 g of Vc raw powder is splashed in a full pool before and after the sudden change of weather (cold flow, strong wind, high temperature and the like) in each mu of land. Reeds can be planted in the saline-alkali soil around the aquaculture pond to attract some birds to lay eggs, so that habitat is increased, the birds can timely clean diseased shrimps and weak shrimps floating on the water surface, and the method is also one of effective means for preventing and controlling disease organisms.

7) And natural illumination is carried out in the whole culture period.

8) And collecting the shrimps in a wheel way, and culturing for 50-60 days, wherein the shrimps can be collected by using a labyrinth or a ground cage with corresponding meshes according to the requirement of purchasing the shrimp specifications by customers, and the net serving as a disease prevention and control net originally is changed into a net which is 5-10 cm from the bottom of the pond to the water surface.

9) And (3) transportation: all equipment in contact with the organic shrimps is sprayed with 100PPM potassium permanganate aqueous solution for disinfection and is washed clean. The van is required to be insulated; and a glass fiber reinforced plastic barrel with the side length of 70-80 cm, the height of 1.4-1.5 m, the water level of 1.2 m and 1ppm of Vc raw powder are prepared. Four hooks are arranged at four corners of the inner wall of the bottom, four hooks are arranged at four corners of the upper end of the inner wall of the bottom, a mesh bag with a 6-8-mesh lining is arranged in the glass fiber reinforced plastic barrel (the length-width ratio of the mesh bag is 10-15 cm smaller than that of the glass fiber reinforced plastic barrel, and the height of the mesh bag is 15-20 cm smaller than that of the glass fiber reinforced plastic barrel), one side edge of the mesh bag is opened and closed by a zipper or a felt, the layering interval of drawer type with the height of about 15cm is set (the prawn benthic habit can fully utilize the space, and the layering can reduce the mutual damage of thorns in the swimming of the prawns), the lining mesh bag completely opens nanotubes in the mesh bag to inflate through the hooks at 8 corners, 10-20 g of active carbon is added to each layer of drawer, the water temperature is 2-2.5 lower than that of the barrel, the water.

10) And processing: the production and processing environment meets the GB 14881 regulation, and the processing water for organic aquatic products meets the requirements of the Standard for Drinking Water for Life (CB 5749-1985); the seawater used should meet the specification of class 1 in seawater quality Standard (CB 3077-1997); the ice used in the production process should meet the requirements of artificial ice (SC/F9001-1984).

5. Cleaning: and (3) cleaning the qualified shrimps by using ice water with the temperature of below 10 ℃ for 30-50 seconds to remove mud, crushed ice, weeds and the like attached to the surfaces of the raw materials.

6. Preparation of flake ice: the ice making water of the flake ice machine is pure water. The sanitary condition of the flake ice machine is checked every day, and various sanitary hidden dangers are eliminated in time. Transporting by using a special ice storage barrel; the flake ice between the rough machining and the fine machining can not be used alternately.

7. And (4) preserving the flake ice: evenly paving ice on the bottom layer of the heat-preserving barrel, and then flatly preserving the shrimps in a layer by layer and covering.

8. Removing heads: the head of the shrimp is removed from bottom to top, the throat meat is kept, and the shrimp membrane and the shrimp paste are removed completely.

9. Cleaning: the shrimp with heads removed is cleaned in a cleaning machine by using ice water with the temperature below 10 ℃ for about 20 to 40 seconds, and the residual shrimp paste and the like are cleaned.

10. Sorting in a grading manner: grading according to the conventional count or the count range required by a client, and picking out unqualified products such as discolored products, broken sections, damaged products and the like.

11. Sorting in a grading manner: the original stored headless shrimp raw materials are unfrozen by a shrimp melting machine, and the temperature of the unfrozen water is controlled below 4 ℃.

12. Shelling/opening back/picking intestine: removing shrimp shells and shrimp legs by a manual or husking machine, keeping tail meat, adding ice in a heat preservation barrel for temporary storage after husking by the husking machine, then selecting to leave or not leave a phoenix tail, selecting intestines or not selecting the intestines, opening or not opening the back, selecting and lifting from a second section of the tail of the shrimp back, wherein a needle opening cannot be too large, opening the back slightly from a first section of the shrimp to a fifth section of the shrimp, and not opening too deeply during opening the back, and the shrimp body can not be damaged only by slightly scratching the outer layer of the shrimp back.

13. Inspecting/cleaning: and (4) putting the processed product on a workbench for inspection, retreating and re-processing the product which does not meet the processing requirement, and putting the processed qualified product into a shrimp washer for cleaning.

14. And (4) secondary inspection: and (4) placing the cleaned product on a goods inspection table for inspection, and picking out impurities such as dried small shrimps, shrimp claws, intestinal glands and shrimp, and products which do not meet the specification requirement. And then putting the qualified product into a heat-preserving barrel, and preserving the shrimps in a layer by layer with ice and dry ice.

15. And (3) sterilization: sterilizing the product in the heat-insulating barrel with ozone water, and draining.

16. Single freezing: uniformly spreading the drained product on a plate belt, ensuring that the quick-freezing temperature is below-30 ℃, and keeping the center of the quickly-frozen shrimp body at a temperature below-18 ℃.

17, weighing: and carrying out metering weighing according to the requirements of customers.

18. Plating ice clothes: according to the order requirement, the temperature of the ice coating is measured once every 1 hour after the ice coating is plated by ozone water with the temperature below 3 degrees, and the ice coating rate is measured once every 30 minutes.

19. And (4) checking and accepting the packaging materials: the packaging material comes from a manufacturer with legal production qualification, and a quality manager inspects the qualification certificates of the manufacturer such as production licenses, type inspection reports and the like, and simultaneously, the packaging material is approved to receive after inspecting the sanitation and quality of goods to meet the requirements.

20. And (4) packing material storage, namely, immediately transferring the qualified packing materials which are inspected to a special packing material warehouse, piling the qualified packing materials on the ground away from a wall, and specially storing the qualified packing materials in the inner packing material warehouse and the outer packing material warehouse, wherein the packing materials are specially used for the inner packing material warehouse and the outer packing material warehouse and the packing materials comply with the first-in first-out.

21. Inner package sterilization: sterilizing with ultraviolet lamp before use.

22. Inner packaging: the packaging material is used according to the requirements of customers, the air in the bag is exhausted as much as possible before packaging and sealing, and the sealing is tight and beautiful.

23. Metal probing (CCP 2): the metal detector is calibrated by using a standard metal block before use, every hour in the middle of production and before shutdown, and the product passes through the metal detector bag by bag to detect whether metal fragments are mixed.

24. Overwrap (CCP 3): the surface of the outer packing box is printed with information such as food allergens and the like, the arrangement is neat when the box is packed, the labels of the inner and outer packages are in accordance, the surface of the box is sealed by sealing glue, a packing belt is packed according to the requirement of a client, and each box is printed with a production batch number.

25. And (3) storage in a cold storage: all finished products are stored in a warehouse according to the first-in first-out principle, the temperature of a refrigeration house is set to be 20 ℃ below zero, the stacking is standard and regular, and the marks are clear.

26. Shipping: the finished product loading and transporting carriage is provided with a refrigerating system, the finished product loading and transporting carriage can be loaded after the refrigerating system is normally operated through the inspection of a quality manager, the cleaning condition of the carriage is simultaneously checked, the food sanitation and safety requirements are met, the finished product loading and transporting carriage is started and refrigerated in time after the loading is finished, and the temperature of the transporting carriage is controlled below-18 ℃.

Compared with the prior art, the invention has the following advantages: the processing method of the invention improves the processing quality of the shelled shrimps; the organic shrimp meat is fed by the artemia in the whole process, so that high-quality basic bait is provided for the shrimp seeds, the feed coefficient is reduced, the water pollution is reduced, the survival rate of the shrimp seeds can be increased, and the growth speed is accelerated.

Detailed Description

Example 1

1. Cleaning: and (3) cleaning the qualified shrimps by using ice water with the temperature of below 10 ℃ for 30-50 seconds to remove mud, crushed ice, weeds and the like attached to the surfaces of the raw materials.

2. Preparation of flake ice: the ice making water of the flake ice machine is pure water. The sanitary condition of the flake ice machine is checked every day, and various sanitary hidden dangers are eliminated in time. Transporting by using a special ice storage barrel; the flake ice between the rough machining and the fine machining can not be used alternately.

3. And (4) preserving the flake ice: evenly paving ice on the bottom layer of the heat-preserving barrel, and then flatly preserving the shrimps in a layer by layer and covering.

4. Removing heads: the head of the shrimp is removed from bottom to top, the throat meat is kept, and the shrimp membrane and the shrimp paste are removed completely.

5. Cleaning: the shrimp with heads removed is cleaned in a cleaning machine by using ice water with the temperature below 10 ℃ for about 20 to 40 seconds, and the residual shrimp paste and the like are cleaned.

6. Sorting in a grading manner: grading according to the conventional count or the count range required by a client, and picking out unqualified products such as discolored products, broken sections, damaged products and the like.

7. Sorting in a grading manner: the original stored headless shrimp raw materials are unfrozen by a shrimp melting machine, and the temperature of the unfrozen water is controlled below 4 ℃.

8. Shelling/opening back/picking intestine: removing shrimp shells and shrimp legs by a manual or husking machine, keeping tail meat, adding ice in a heat preservation barrel for temporary storage after husking by the husking machine, then selecting to leave or not leave a phoenix tail, selecting intestines or not selecting the intestines, opening or not opening the back, selecting and lifting from a second section of the tail of the shrimp back, wherein a needle opening cannot be too large, opening the back slightly from a first section of the shrimp to a fifth section of the shrimp, and not opening too deeply during opening the back, and the shrimp body can not be damaged only by slightly scratching the outer layer of the shrimp back.

9. Inspecting/cleaning: and (4) putting the processed product on a workbench for inspection, retreating and re-processing the product which does not meet the processing requirement, and putting the processed qualified product into a shrimp washer for cleaning.

10. And (4) secondary inspection: and (4) placing the cleaned product on a goods inspection table for inspection, and picking out impurities such as dried small shrimps, shrimp claws, intestinal glands and shrimp, and products which do not meet the specification requirement. And then putting the qualified product into a heat-preserving barrel, and preserving the shrimps in a layer by layer with ice and dry ice.

11. And (3) sterilization: sterilizing the product in the heat-insulating barrel with ozone water, and draining.

12. Single freezing: uniformly spreading the drained product on a plate belt, ensuring that the quick-freezing temperature is below-30 ℃, and keeping the center of the quickly-frozen shrimp body at a temperature below-18 ℃.

13. Metering and weighing: and carrying out metering weighing according to the requirements of customers.

14. Plating ice clothes: according to the order requirement, the temperature of the ice coating is measured once every 1 hour after the ice coating is plated by ozone water with the temperature below 3 degrees, and the ice coating rate is measured once every 30 minutes.

15. And (4) checking and accepting the packaging materials: the packaging material comes from a manufacturer with legal production qualification, and a quality manager inspects the qualification certificates of the manufacturer such as production licenses, type inspection reports and the like, and simultaneously, the packaging material is approved to receive after inspecting the sanitation and quality of goods to meet the requirements.

16. And (4) packing material storage, namely, immediately transferring the qualified packing materials which are inspected to a special packing material warehouse, piling the qualified packing materials on the ground away from a wall, and specially storing the qualified packing materials in the inner packing material warehouse and the outer packing material warehouse, wherein the packing materials are specially used for the inner packing material warehouse and the outer packing material warehouse and the packing materials comply with the first-in first-out.

17. Inner package sterilization: sterilizing with ultraviolet lamp before use.

18. Inner packaging: the packaging material is used according to the requirements of customers, the air in the bag is exhausted as much as possible before packaging and sealing, and the sealing is tight and beautiful.

19. Metal probing (CCP 2): the metal detector is calibrated by using a standard metal block before use, every hour in the middle of production and before shutdown, and the product passes through the metal detector bag by bag to detect whether metal fragments are mixed.

20. Overwrap (CCP 3): the surface of the outer packing box is printed with information such as food allergens and the like, the arrangement is neat when the box is packed, the labels of the inner and outer packages are in accordance, the surface of the box is sealed by sealing glue, a packing belt is packed according to the requirement of a client, and each box is printed with a production batch number.

21. And (3) storage in a cold storage: all finished products are stored in a warehouse according to the first-in first-out principle, the temperature of a refrigeration house is set to be 20 ℃ below zero, the stacking is standard and regular, and the marks are clear.

22. Shipping: the finished product loading and transporting carriage is provided with a refrigerating system, the finished product loading and transporting carriage can be loaded after the refrigerating system is normally operated through the inspection of a quality manager, the cleaning condition of the carriage is simultaneously checked, the food sanitation and safety requirements are met, the finished product loading and transporting carriage is started and refrigerated in time after the loading is finished, and the temperature of the transporting carriage is controlled below-18 ℃.

The above-described embodiments are merely preferred embodiments of the present invention, and all equivalent changes or modifications of the structures, features and principles described in the claims of the present invention are included in the scope of the present invention.