阅读说明：本技术 蛙类离体心脏心电检测辅助装置 (Frog class separation heart electrocardio detects auxiliary device ) 是由 李秀国 于 2021-03-11 设计创作，主要内容包括：本发明公开了一种蛙类离体心脏心电检测辅助装置,它由夹层式底座、底座上设的储液池、储液池上设有的上设中央孔的盖板、储液池底面上设的中央转台放置槽、中央转台放置槽上设有的中央设有中心孔的中央转台、位于底座上且套设在储液池外侧的转盘、设在转盘上的支架、底座和储液池之间设有的心电检测输出装置组成。本发明能够满足在不触碰心脏的条件下调节心脏和引导电极之间的距离和角度,不仅为单纯离体心脏的心电向量环测定、心电轴实验、心肌损伤心电和心电容积导电等的实验教学和研究提供了科学合理的实验条件,还适于检测蛙心灌流离体心脏在不同给药处理前后的心电变化,扩大了其在实验教学和科研中的应用范围。(The invention discloses a frog off-body cardiac electrocardiograph detection auxiliary device, which consists of a sandwich type base, a liquid storage tank arranged on the base, a cover plate arranged on the liquid storage tank and provided with a central hole, a central rotary table placing groove arranged on the bottom surface of the liquid storage tank, a central rotary table arranged on the central rotary table placing groove and provided with a central hole in the center, a rotary table positioned on the base and sleeved outside the liquid storage tank, a support arranged on the rotary table, and an electrocardiograph detection output device arranged between the base and the liquid storage tank. The invention can adjust the distance and angle between the heart and the guide electrode under the condition of not touching the heart, not only provides scientific and reasonable experimental conditions for the electrocardiogram vector loop measurement of the isolated heart, the electrocardiogram axis experiment, the myocardial damage electrocardiogram, the electrocardiogram volume conduction experiment and the like, but also is suitable for detecting the electrocardiogram change of the frog heart perfusion isolated heart before and after different administration treatments, and enlarges the application range of the frog heart perfusion isolated heart in the experimental teaching and scientific research.)

1. The frog off-body cardiac electrocardiographic detection auxiliary device is characterized by comprising a sandwich type base, a liquid storage tank arranged on the base, a cover plate arranged on the liquid storage tank and provided with a central hole, a central rotary table placing groove arranged on the bottom surface of the liquid storage tank, a central rotary table arranged on the central rotary table placing groove and provided with a central hole, a rotary table positioned on the base and sleeved outside the liquid storage tank, a support arranged on the rotary table, and an electrocardiographic detection output device arranged between the base and the liquid storage tank.

2. The device for assisting in the isolated cardiac electrocardiographic examination of a frog in accordance with claim 1, wherein the electrocardiographic examination output device comprises seventeen wiring terminals and nineteen guide electrodes arranged on a base, metal leads positioned in a base interlayer are connected between the guide electrodes and the wiring terminals, twelve angle marking lines are arranged on the bottom surface of the liquid storage pool, the angle marking lines are evenly carved by taking the center point of the bottom surface of the liquid storage pool as the center, one of the nineteen guide electrodes is arranged at the center point of the angle marking line, sixteen guide electrodes are respectively arranged at 0 degree, 90 degree, 180 degree and 270 degree directions of the angle marking line from the center point 1.5, 3.0, 4.5 and 6.0 cm, the remaining two guide electrodes are respectively arranged at 210 degree and 330 degree directions of the angle marking line from the center point 6.0 cm, the guide electrodes at 90 degree, 210 degree and 330 degree directions of the angle marking line from the center point 6.0 cm are connected to the same metal lead as a middle lead in the base interlayer The electrocardio end electrode, other sixteen leading electrodes are connected with a metal wire respectively, seventeen metal wires are connected with seventeen corresponding wiring terminals respectively in the interlayer of the base.

3. The device as claimed in claim 1, wherein the upper surface of the central turntable is slightly concave and has two protrusions in a symmetrical manner.

4. The device as claimed in claim 1, wherein the base comprises an upper bottom surface, a lower bottom surface, and a sidewall between the upper bottom surface and the lower bottom surface, and seventeen terminals are disposed on the sidewall.

5. The device as claimed in claim 1, wherein the cover is transparent for easy observation.

Technical Field

The invention relates to a life science research and experiment teaching related device, in particular to a frog off-body cardiac electrocardiogram detection auxiliary device.

Background

Unlike mammals, the isolated heart of frog can maintain normal physiological function for a long time in physiological saline solution at room temperature without oxygen supply equipment, and is widely applied to experimental teaching and scientific research in medicine, biology, pharmacy and the like. At present, frog electrocardio detection is usually carried out by an in-vivo experiment and an in-vitro experiment. In vivo experiments, after the toad generally destroys brain and marrow and exposes heart, the electrocardiogram is detected by connecting an electrocardiogram lead wire to a needle electrode inserted under the body epidermis or a probing electrode contacted with the heart, the electrocardiogram is usually used for judging the direction of an electrocardiogram axis, and electrocardiogram characteristics and the like after premature contraction and injury are observed and analyzed; in the in vitro experiment, an in vitro heart is directly placed in a plate filled with the ringer's solution, and the electrocardio is detected in a mode that the side wall of the plate is clamped by an alligator clamp of an electrocardio-conductive connecting line, so that the in vitro heart is commonly used for an electrocardio-volume conduction experiment. Both of these two detection methods have certain disadvantages in practical applications: firstly, myoelectric interference is easily caused by improper brain and marrow destruction in an in vivo experiment, and the waveform is too small due to the fact that the crocodile clip for clamping the plate is far away from the heart in an in vitro experiment, so that the stability and sensitivity of the electrocardiographic waveform are affected; secondly, in order to avoid the influence of human factors on the electrocardiographic detection, the distance and the angle between the heart and the guide electrode should be adjusted under the condition of avoiding moving and touching the heart as far as possible in the electrocardiographic characteristic detection process, and the requirements are difficult to be simultaneously ensured in the traditional in-vitro experiment and the traditional in-vivo experiment; thirdly, the traditional frog electrocardio detection experiment method is not beneficial to observing the electrocardio change before and after administration.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provide the auxiliary device for the ex-vivo heart electrocardio detection of the frogs, which is convenient for changing the distance and the angle between the guide electrode and the heart under the condition of not touching the heart, has more stable and sensitive electrocardio waveform and is suitable for observing the electrocardio change before and after administration.

In order to achieve the purpose, the invention adopts the following technical scheme: the electrocardiogram detection device comprises a sandwich type base, a liquid storage tank arranged on the base, a cover plate arranged on the liquid storage tank and provided with a central hole, a central rotary table placing groove arranged on the bottom surface of the liquid storage tank, a central rotary table arranged on the central rotary table placing groove and provided with a central hole in the center, a rotary table positioned on the base and sleeved outside the liquid storage tank, a support arranged on the rotary table, and an electrocardiogram detection output device arranged between the base and the liquid storage tank.

The electrocardio detection output device comprises seventeen wiring terminals and nineteen guide electrodes which are arranged on a base, metal leads positioned in a sandwich layer of the base are connected between the guide electrodes and the wiring terminals, twelve angle marking lines are arranged on the bottom surface of a liquid storage tank, the angle marking lines are formed by uniformly carving the center of the bottom surface of the liquid storage tank, one guide electrode of the nineteen guide electrodes is arranged at the center of the angle marking line, sixteen guide electrodes are respectively arranged at the positions 1.5, 3.0, 4.5 and 6.0 cm away from the center in the directions of 0 degree, 90 degree, 180 degree and 270 degree of the angle marking line, the remaining two guide electrodes are respectively arranged at the positions 6.0 cm away from the center in the directions of 210 degree, 90 degree, 210 degree and 330 degree of the angle marking line, the guide electrodes at the positions of 210 degree and 330 degree of the angle marking line are connected to the same metal lead in the sandwich layer of the base as a central electric end electrode, the other sixteen conducting electrodes are respectively and independently connected with a metal lead, and seventeen metal leads are respectively connected with seventeen corresponding connecting terminals in the interlayer of the base.

The upper plane of the central turntable is slightly concave and is provided with two bulges in a symmetrical mode.

The base comprises last bottom surface, lower bottom surface and last bottom surface and the lateral wall between the bottom surface down, seventeen binding post establish on the lateral wall.

The cover plate is designed to be transparent so as to be convenient for observation.

Due to the adoption of the technical scheme, the invention has the following advantages:

1. the frog in-vitro heart electrocardio detection auxiliary device avoids factors such as myoelectricity interference in body electrocardio detection, can adjust the distance between the guide electrode and the in-vitro heart to be very close, and can obviously improve the stability and the sensitivity of the detected electrocardio curve waveform;

2. the central rotary table and the rotary table on the frog in-vitro heart electrocardio detection auxiliary device can rotate randomly, and the distance between the guide electrode and the central rotary table is adjustable, so that the requirement of adjusting the distance and the angle between the heart and the guide electrode under the condition of not touching the heart can be met, and more scientific and reasonable experimental conditions are provided for experimental teaching and related researches of electrocardio vector loop measurement, an electrocardio axis experiment, cardiac muscle damage electrocardio detection, electrocardio volume conduction and the like;

3. the frog isolated heart electrocardio detection auxiliary device is not only suitable for detecting the electrocardio of a single isolated heart, but also suitable for detecting the electrocardio change of a frog heart perfusion isolated heart before and after different drug administration treatments, thereby expanding the application range of the frog isolated heart electrocardio detection auxiliary device in experimental teaching and scientific research.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of the bottom of the reservoir of the present invention;

FIG. 3 is a schematic diagram of the application of the present invention in the simple ex vivo cardiac electrocardiographic examination of frogs;

FIG. 4 is an application schematic diagram of the frog heart perfusion in-vitro heart electrocardiographic examination of the invention after a frog heart tube and a liquid feeding tube are arranged on a bracket.

In the figure: 1. the device comprises a support, 2, a central hole, 3, a cover plate, 4, a protrusion, 5, a central turntable, 6, a turntable, 7, a liquid storage tank, 8, a central turntable placing groove, 9, the bottom surface of the liquid storage tank, 10, a base, 11, a side wall, 12, a wiring terminal, 13, a guide electrode, 14, an angle marking line, 15, an isolated heart, 16, a frog heart tube, 17, a double-concave clamp, 18, a liquid adding tube, 19, a fixing rod, 20 and an electrocardiogram lead wire.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

As shown in fig. 1 and 2, the auxiliary device for extracorporeal cardiac electrocardiography detection of frogs comprises a sandwich-type base 10, a liquid storage tank 7 arranged on the base 10, a cover plate 3 provided with a central hole 2 on the liquid storage tank 7, a central turntable placement groove 8 arranged on the bottom surface of the liquid storage tank 7, a central turntable 5 provided with a central hole in the center on the central turntable placement groove 8, a turntable 6 positioned on the base 10 and sleeved outside the liquid storage tank 7, a support 1 arranged on the turntable 6, and an electrocardiography detection output device arranged between the base 10 and the liquid storage tank 7. The electrocardiograph detection output device comprises seventeen wiring terminals 12 and nineteen guide electrodes 13 which are arranged on a base 10, metal leads positioned in a interlayer of the base 10 are connected between the guide electrodes 13 and the wiring terminals 12, twelve angle mark lines 14 are arranged on a bottom surface 9 of a liquid storage pool, the angle mark lines 14 are uniformly carved by taking a center point of the bottom surface 9 of the liquid storage pool as a center, one guide electrode of the nineteen guide electrodes 13 is arranged on a center point of the angle mark line 14, sixteen guide electrodes are respectively arranged at the positions of 0 degree, 90 degree, 180 degree and 270 degree of the angle mark line 14 from the center point by 1.5, 3.0, 4.5 and 6.0 cm, the rest two guide electrodes are respectively arranged at the positions of 210 degree and 330 degree of the angle mark line 14 from the center point by 6.0 cm, the guide electrodes of 90 degree, 210 degree and 330 degree of the angle mark line 14 from the center point by 6.0 cm are connected to the same metal lead in the interlayer of the base as a central electric terminal electrode, the other sixteen conducting electrodes are respectively and independently connected with a metal lead, and seventeen metal leads are respectively connected with seventeen corresponding connecting terminals in the interlayer of the base. The upper plane of said central turret 5 is slightly concave and provided with two projections 4 in a symmetrical manner. The base 10 is composed of an upper bottom surface, a lower bottom surface and a side wall 11 between the upper bottom surface and the lower bottom surface, and seventeen wiring terminals 12 are arranged on the side wall 11. The cover plate 3 is designed to be transparent so as to be convenient for observation.

The working principle of the invention is as follows:

when the simple frog extracorporeal heart electrocardiogram detection is carried out, as shown in the principle of fig. 3, a proper amount of wilted liquid is added into the liquid storage tank 7, so that the wilted liquid can submerge all the guide electrodes 13 on the bottom surface 9 of the liquid storage tank, and the central rotary table 5 is placed in the central rotary table placing groove 8.

The signal input end of the electrocardio lead wire 20 is connected to a corresponding channel of the PowerLab or BL-420 biological function experiment system and is used for recording electrocardio.

Taking a toad or a frog, destroying brain and marrow, preparing an isolated heart 15, placing the heart on the central rotary table 5, adjusting the amount of the wilsonite liquid in the liquid storage tank 7 to ensure that the isolated heart 15 does not drift along with the slight rotation of the central rotary table 5, and covering the cover plate 3.

When the influence of the change of the position of the cathode of the guide electrode on the electrocardiographic waveform is detected, the glass minute needle extends into the liquid storage tank 7 from the central hole 2, the bulge 4 on the central rotary table 5 is slightly shifted, so that the apex of the heart 15 in vitro faces the 0-degree direction of the angle mark line 14, the ground wire of the electrocardiographic lead line 20 is connected to the wiring terminal 12 of the guide electrode 13 with the distance from the 270-degree direction of the angle mark line 14 to the central point being 6 cm, the anode of the electrocardiographic lead line 20 is connected to the wiring terminal 12 of the guide electrode 13 with the distance from the 0-degree direction of the angle mark line 14 to the central point being 1.5 cm, the cathode of the electrocardiographic lead line 20 is connected to other wiring terminals 12, and the electrocardiographic waveform when the distance and the angle of the cathode are changed under the condition that the.

During an electrocardiographic volume conduction experiment, the apical direction of an isolated heart 15 and the ground wire connecting position of an electrocardiographic lead 20 are arranged in the same way, the cathode of the electrocardiographic lead 20 is connected to a connecting terminal 12 of a guide electrode 13 which is 1.5 cm away from the central point in the 180-degree direction of an angle mark line 14, then the anode of the electrocardiographic lead 20 is sequentially connected to the connecting terminal 12 of the guide electrode 13 which is 1.5, 3.0, 4.5 and 6 cm away from the central point in the 0-degree direction of the angle mark line 14, the electrocardiographic waveform along with the change of the distance between a probe electrode and the heart is recorded, the anode of the electrocardiographic lead 20 is connected to the connecting terminal 12 of the guide electrode 13 which is 1.5 cm away from the central point in the 0-degree direction of the angle mark line 14, a glass minute needle extends into a liquid storage tank 7 from a central hole 2, a bulge 4 on a central rotary table 5 is slightly shifted in the counterclockwise direction, so that the apical direction sequentially faces twelve angle mark lines 14, the electrocardiographic waveform is recorded as the relative angle between the probe electrode and the heart changes.

When the frontal plane electrocardio-axis and the electrocardio-vector ring are judged, the frontal plane electrocardio-axis direction and the electrocardio-vector ring can be measured and calculated according to the electrocardio-waveform measured by rotating the bulge 4 on the central turntable 5 in the electrocardio volume conducting experiment.

When detecting the premature contraction and compensating the intermittent electrocardiographic waveform, a glass minute needle is extended into a liquid storage tank 7 from a central hole 2, and a bulge 4 on a central rotary table 5 is slightly rotated, so that the apex of the isolated heart 15 is just opposite to the 0-degree direction of an angle mark line 14, the ground wire of an electrocardiographic lead line 20 is connected to a connecting terminal 12 of a guide electrode 13 which is 6 cm away from the central point in the 270-degree direction of the angle mark line 14, the positive electrode of the electrocardiographic lead line 20 is connected to the connecting terminal 12 of the guide electrode 13 which is 1.5 cm away from the central point in the 0-degree direction of the angle mark line 14, the negative electrode of the electrocardiographic lead line 20 is connected to the connecting terminal 12 of the guide electrode 13 which is 1.5 cm away from the central point in the 180-degree direction of the angle mark line 14, and the recording glass minute needle stimulates the electrocardiographic before and after the.

When an experiment for detecting the electrocardio-characteristics of the injury is carried out, the connecting position of the electrocardio-lead wire 20 is synchronously contracted and compensated for intermittent experiments, a glass minute needle is extended into the liquid storage tank 7 from the central hole 2, and the bulge 4 on the central rotary table 5 is slightly shifted, so that the heart chamber wall of the part to be injured of the isolated heart 15 is opposite to the positive electrode of the guide electrode 13 (namely the direction of 0 degree of the angle mark line 14), and then the electrocardiogram before and after the heart chamber wall is burnt by the glass minute needle is recorded.

When the frog heart perfusion isolated heart electrocardiographic examination is carried out, as shown in the principle of fig. 4, a rotating disc 6 is placed on the periphery of a liquid storage tank 7, a fixed rod 19 is fixed on a support 1 by a double-concave clamp 17, a frog heart perfusion specimen by a single-tube method is prepared by a frog heart tube 16 (patent number: ZL 201820357075.9) with a slightly bent tip and a liquid adding tube 18, the frog heart tube 16 is connected to the fixed rod 19, and the height of the frog heart tube 16 is adjusted, so that the isolated heart 15 is horizontally placed on a central rotating table 5.

During frog heart perfusion isolated heart electrocardio detection, the apex direction of an isolated heart 15 is adjusted by rotating the rotary disc 6, liquid is added or extracted into the frog heart tube 16 through the liquid adding tube 18, and the electrocardio before and after the change of perfusate components is recorded.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all simple modifications, equivalent changes and modifications made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.