阅读说明：本技术 一种基于香樟叶的白绢病天然杀菌剂及其制备方法 (Natural southern blight bactericide based on cinnamomum camphora leaves and preparation method thereof ) 是由 刘训悦 时浩杰 刘鹏 饶琼 郭龙飞 徐笑笑 于 2020-12-28 设计创作，主要内容包括：本发明公开了一种基于香樟叶的白绢病天然杀菌剂及其制备方法,其原料按重量份比包括：香樟叶40-50份、乙醇20-30份、八角4-6份、紫苏叶6-8份、姜黄2-3份和牵牛子12-15份,所述香樟叶在使用前需要先将其表面的杂物进行清理,然后对其进行杀菌处理,所述八角、紫苏叶、姜黄和牵牛子在使用前需要通过水分进行清洗,清洗时避免物料破损,本发明涉及白绢病杀菌剂技术领域。该基于香樟叶的白绢病天然杀菌剂及其制备方法,全部是由天然材料制成,原料简单易得,且制作步骤简单,大大的降低了使用者的制备成本,且制备的杀菌剂能够很好的对白绢病进行杀菌,保证了作物的正常生长,且提前喷洒可一定程度上避免作物发生感染,整体防护性强,治愈率高。(The invention discloses a natural bactericide for southern blight based on camphor leaves and a preparation method thereof, wherein the natural bactericide comprises the following raw materials in parts by weight: the invention relates to a Chinese medicine bactericide, which comprises, by weight, 40-50 parts of cinnamomum camphora leaves, 20-30 parts of ethanol, 4-6 parts of anise, 6-8 parts of perilla leaves, 2-3 parts of turmeric and 12-15 parts of pharbitis seeds. The natural southern blight bactericide based on the cinnamomum camphora leaves and the preparation method thereof are all made of natural materials, the raw materials are simple and easy to obtain, the preparation steps are simple, the preparation cost of a user is greatly reduced, the prepared bactericide can well sterilize the southern blight, the normal growth of crops is ensured, the infection of the crops can be avoided to a certain extent by spraying in advance, the overall protection performance is strong, and the cure rate is high.)

1. A natural sclerotium rolfsii bactericide based on cinnamomum camphora leaves is characterized in that: the raw materials comprise the following components in parts by weight: 40-50 parts of camphor leaves, 20-30 parts of ethanol, 4-6 parts of anise, 6-8 parts of perilla leaves, 2-3 parts of turmeric and 12-15 parts of pharbitis seeds.

2. The natural bactericide for southern blight based on cinnamomum camphora leaves according to claim 1, which is characterized in that: before the camphor leaves are used, sundries on the surfaces of the camphor leaves need to be cleaned firstly, and then the camphor leaves are sterilized.

3. The natural bactericide for southern blight based on cinnamomum camphora leaves according to claim 1, which is characterized in that: anise, perilla leaf, turmeric and morning glory seed need wash through moisture before using, avoid the material damaged during the washing, the material in the material can mix with moisture like this, washs and accomplishes the back and carry out drying process to it through the drying-machine, then the laying is put the back and is disinfected through sterilamp, place after continuously disinfecting 12 hours for later use.

4. The natural bactericide for southern blight based on cinnamomum camphora leaves according to any one of claims 1 to 3, which is characterized in that: the preparation method specifically comprises the following steps:

s1, putting the sterilized camphor leaves into an extraction box, adding 60% ethanol in parts by weight, and controlling the ratio of materials to liquid to be 1: 10, leaching the camphor leaves by a water bath method for 12 hours at the extraction temperature of 80 ℃;

s2, putting the cleaned star anise, perilla leaf, turmeric and pharbitis seed into a mixing container, adding 95% ethanol water solution in parts by weight, heating to 60 ℃, decocting for 3 hours, filtering the decocted solution, collecting filtrate, adding 95% ethanol water solution in parts by weight into filter residues, heating to 60 ℃, decocting for 3 hours, filtering, mixing the filtrate obtained in the first time and the filtrate obtained in the filter residues, adding a proper amount of water, and naturally standing for one day;

s3, putting the camphor leaf extract prepared in the S1 into a stirrer, adding the filtrate prepared in the S2, starting the stirrer to mix, controlling the rotating speed of the stirrer to be 180 revolutions per minute, continuously mixing for one hour, cooling to room temperature, removing floating foam and residues on the surface of the mixed solution, completely removing the mixture, starting the stirrer again to stir for 1 hour, adding proper water when the liquid is thick in the stirring process, cooling to room temperature after stirring, and standing for later use;

and S4, filling the cooled material in the step S3 by a filling machine, checking whether the material is damaged or overflows, and obtaining the natural bactericide after the material is checked to be qualified.

5. The preparation method of the natural southern blight bactericide based on cinnamomum camphora leaves as claimed in claim 4, wherein the natural southern blight bactericide comprises the following steps: in the S1, the camphor leaves are subjected to extraction treatment twice, 10g of sterilized camphor leaves are selected for the first time, then 100ml of 59.85% by weight of ethanol is added, 38ml of ethanol is left after volatilization after 12 hours of water bath extraction, the constant volume of 59.85% ethanol is 50ml, 100ml of ethanol is added for the second time, 26ml of ethanol is left after volatilization after 12 hours of water bath extraction, and the constant volume of 60% ethanol is 30 ml.

6. The preparation method of the natural southern blight bactericide based on cinnamomum camphora leaves as claimed in claim 4, wherein the natural southern blight bactericide comprises the following steps: in S3, when the camphor leaf extract and the filtrate are mixed by a stirrer, it is necessary to avoid the infection of the materials.

Technical Field

The invention relates to the technical field of southern blight bactericides, in particular to a southern blight natural bactericide based on cinnamomum camphora leaves and a preparation method thereof.

Background

Southern blight is also known as sclerotic root rot and sclerotic seedling blight, which damages the root and stem of nursery stock and saplings. The disease can damage various plants, mainly various trees such as camellia oleifera, aleurites fordii, catalpa bungei, citrus, apples, phoenix trees, paulownia, walnuts, pinus massoniana and the like, and can cause diseases of many crops such as peanuts, hot peppers, green Chinese onions and the like. The sclerotium rolfsii is a root-inhabiting bacterium, mycelia or sclerotia overwinter in soil or on diseased roots, new mycelia are generated when the temperature is proper in the next year, germs can spread along with surface water flow in the soil, the mycelia spread in the soil by means of growth to infect the roots or roots of nursery stocks, and after the nursery stocks are damaged, the absorption of water and nutrients is influenced, so that the seedlings grow badly, leaves on the ground become smaller and yellow, the internodes of branches and tips are shortened, the branches and leaves wither when serious, and the whole plant withers after the lesion ring and stems are one week. Under humid conditions, the surface of the damaged rhizome or the soil near the ground is covered with white sericite mycelium. In the later period, a lot of small sclerotia in the shape of rapeseeds are formed in the mycelium, the mycelium is white at first, then gradually changes from light yellow to yellow brown, and then changes into dark brown. The existing southern blight exists widely, and in order to treat the southern blight, a southern blight bactericide needs to be used, and the southern blight bactericide is various in types, but has some problems.

Traditional southern blight natural bactericide, because whole is natural composition, lead to bactericidal effect not very good, most just play an inhibitory action, after restricting southern blight growth in the short time, the same serious of the short period southern blight again, some bactericidal rate is not very good, can only kill a small portion of germ after disinfecting, and whole step is loaded down with trivial details moreover, and the producer of being inconvenient for prepares, and holistic economic benefits is not very high.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a natural southern blight bactericide based on cinnamomum camphora leaves and a preparation method thereof, and solves the problems that the whole curing effect of the natural southern blight bactericide is not good and the protection effect is not good.

In order to achieve the purpose, the invention is realized by the following technical scheme: a natural bactericide for southern blight based on camphor leaves and a preparation method thereof are disclosed, which comprises the following raw materials in parts by weight: 40-50 parts of camphor leaves, 20-30 parts of ethanol, 4-6 parts of anise, 6-8 parts of perilla leaves, 2-3 parts of turmeric and 12-15 parts of pharbitis seeds.

Preferably, the camphor leaves need to be cleaned of impurities on the surfaces before use, and then are sterilized.

Preferably, anise, perilla leaf, turmeric and morning glory seed need wash through moisture before using, avoid the material damaged during the washing, and the material in the material can mix with moisture like this, carries out drying process to it through the drying-machine after the washing is accomplished, then puts the back through sterilamp and disinfects flatly, places after continuously disinfecting 12 hours for later use.

The invention also discloses a preparation method of the natural southern blight bactericide based on the cinnamomum camphora leaves, which specifically comprises the following steps:

s1, putting the sterilized camphor leaves into an extraction box, adding 60% ethanol in parts by weight, and controlling the ratio of materials to liquid to be 1: 10, leaching the camphor leaves by a water bath method for 12 hours at the extraction temperature of 80 ℃;

s2, putting the cleaned star anise, perilla leaf, turmeric and pharbitis seed into a mixing container, adding 95% ethanol water solution in parts by weight, heating to 60 ℃, decocting for 3 hours, filtering the decocted solution, collecting filtrate, adding 95% ethanol water solution in parts by weight into filter residues, heating to 60 ℃, decocting for 3 hours, filtering, mixing the filtrate obtained in the first time and the filtrate obtained in the filter residues, adding a proper amount of water, and naturally standing for one day;

s3, putting the camphor leaf extract prepared in the S1 into a stirrer, adding the filtrate prepared in the S2, starting the stirrer to mix, controlling the rotating speed of the stirrer to be 180 revolutions per minute, continuously mixing for one hour, and cooling to room temperature;

and S4, filling the cooled material in the step S3 by a filling machine, checking whether the material is damaged or overflows, and obtaining the natural bactericide after the material is checked to be qualified.

Preferably, in S1, the camphor leaves are extracted twice, wherein in the first time, 10g of sterilized camphor leaves are selected, then 100ml of 59.85% by weight of ethanol is added, after 12 hours of water bath extraction, 38ml of ethanol is left after volatilization, the constant volume of 59.85% ethanol is 50ml, in the second time, 100ml of ethanol is added, after 12 hours of water bath extraction, the constant volume of 26ml of ethanol is left after volatilization, and the constant volume of 60% ethanol is 30 ml.

Preferably, in S3, when the camphor leaf extract and the filtrate are mixed by a stirrer, it is necessary to avoid the infection of the materials.

Advantageous effects

The invention provides a natural southern blight bactericide based on camphor leaves and a preparation method thereof. Compared with the prior art, the method has the following beneficial effects:

(1) the natural southern blight bactericide based on the camphor leaves and the preparation method thereof comprise the following raw materials in parts by weight: 40-50 parts of cinnamomum camphora leaves, 20-30 parts of ethanol, 4-6 parts of anise, 6-8 parts of perilla leaves, 2-3 parts of turmeric and 12-15 parts of pharbitis seeds, sundries on the surfaces of the cinnamomum camphora leaves need to be cleaned before use, the anise, the perilla leaves, the turmeric and the pharbitis seeds need to be cleaned through water before use, the materials are prevented from being damaged during cleaning, the materials in the materials are mixed with the water, the materials are dried through a dryer after cleaning, then the materials are horizontally laid and sterilized through an ultraviolet sterilizing lamp, the materials are placed for standby after continuous sterilization for 12 hours, all the materials are made of natural materials, the raw materials are simple and easy to obtain, the manufacturing steps are simple, the preparation cost of a user is greatly reduced, the prepared bactericide can well sterilize southern blight, and the normal growth of crops is guaranteed, moreover, the pesticide is sprayed in advance, so that the crop infection can be avoided to a certain extent, the whole protection performance is strong, the cure rate is high, and the pesticide is convenient for a user to use.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides three technical schemes: a natural southern blight bactericide based on cinnamomum camphora leaves and a preparation method thereof specifically comprise the following embodiments:

example 1

S1, putting 40 parts of sterilized camphor leaves into an extraction box, adding 20 parts of 60% ethanol by mass, and controlling the ratio of materials to liquid to be 1: 10, leaching the camphor leaves by a water bath method for 12 hours at the extraction temperature of 80 ℃;

s2, putting the cleaned 4 parts of anise, 6 parts of perilla leaves, 2 parts of turmeric and 12 parts of morning glory seeds into a mixing container, adding 95% ethanol aqueous solution by mass, heating to 60 ℃, decocting for 3 hours, filtering the decocted solution, collecting filtrate, adding 95% ethanol aqueous solution by mass into filter residues, heating to 60 ℃, decocting for 3 hours, filtering, mixing the filtrate obtained by the first time and the filtrate obtained by the filter residues, adding a proper amount of water, and naturally standing for one day;

s3, putting the camphor leaf extract prepared in the S1 into a stirrer, adding the filtrate prepared in the S2, starting the stirrer to mix, controlling the rotating speed of the stirrer to be 180 revolutions per minute, continuously mixing for one hour, cooling to room temperature, removing floating foam and residues on the surface of the mixed solution, completely removing the mixture, starting the stirrer again to stir for 1 hour, adding proper water when the liquid is thick in the stirring process, cooling to room temperature after stirring, and standing for later use;

and S4, filling the cooled material in the step S3 by a filling machine, checking whether the material is damaged or overflows, and obtaining the natural bactericide after the material is checked to be qualified.

Example 2

S1, putting 45 parts of sterilized camphor leaves into an extraction box, adding 25 parts of 60% ethanol by mass, and controlling the ratio of materials to liquid to be 1: 10, leaching the camphor leaves by a water bath method for 12 hours at the extraction temperature of 80 ℃;

s2, putting the cleaned 5 parts of anise, 6 parts of perilla leaves, 2 parts of turmeric and 14 parts of morning glory seeds into a mixing container, adding 95% ethanol aqueous solution by mass, heating to 60 ℃, decocting for 3 hours, filtering the decocted solution, collecting filtrate, adding 95% ethanol aqueous solution by mass into filter residues, heating to 60 ℃, decocting for 3 hours, filtering, mixing the filtrate obtained by the first time and the filtrate obtained by the filter residues, adding a proper amount of water, and naturally standing for one day;

s3, putting the camphor leaf extract prepared in the S1 into a stirrer, adding the filtrate prepared in the S2, starting the stirrer to mix, controlling the rotating speed of the stirrer to be 180 revolutions per minute, continuously mixing for one hour, cooling to room temperature, removing floating foam and residues on the surface of the mixed solution, completely removing the mixture, starting the stirrer again to stir for 1 hour, adding proper water when the liquid is thick in the stirring process, cooling to room temperature after stirring, and standing for later use;

and S4, filling the cooled material in the step S3 by a filling machine, checking whether the material is damaged or overflows, and obtaining the natural bactericide after the material is checked to be qualified.

Example 3

S1, putting 50 parts of sterilized camphor leaves into an extraction box, adding 30 parts of 60% ethanol by mass, and controlling the ratio of materials to liquid to be 1: 10, leaching the camphor leaves by a water bath method for 12 hours at the extraction temperature of 80 ℃;

s2, putting the cleaned 6 parts of anise, 8 parts of perilla leaves, 3 parts of turmeric and 15 parts of morning glory seeds into a mixing container, adding 95% ethanol aqueous solution by mass, heating to 60 ℃, decocting for 3 hours, filtering the decocted solution, collecting filtrate, adding 95% ethanol aqueous solution by mass into filter residues, heating to 60 ℃, decocting for 3 hours, filtering, mixing the filtrate obtained by the first time and the filtrate obtained by the filter residues, adding a proper amount of water, and naturally standing for one day;

s3, putting the camphor leaf extract prepared in the S1 into a stirrer, adding the filtrate prepared in the S2, starting the stirrer to mix, controlling the rotating speed of the stirrer to be 180 revolutions per minute, continuously mixing for one hour, cooling to room temperature, removing floating foam and residues on the surface of the mixed solution, completely removing the mixture, starting the stirrer again to stir for 1 hour, adding proper water when the liquid is thick in the stirring process, cooling to room temperature after stirring, and standing for later use;

and S4, filling the cooled material in the step S3 by a filling machine, checking whether the material is damaged or overflows, and obtaining the natural bactericide after the material is checked to be qualified.

Effects of the embodiment

The experimental process comprises the following steps: 1. the cure rate is as follows: selecting 30 parts of sclerotium rolfsii germ culture medium with the same mass, dividing 10 samples into one group and three groups, respectively adding the products prepared in the three embodiments into the three groups of culture media to react with sclerotium rolfsii germs, observing and recording the result, observing the actual condition in the culture media after the three groups of reactions are finished, and calculating to obtain the cure rate.

2. The protection effect is as follows: the products prepared in the three examples are respectively sprayed on crops, the crops are cultivated in an environment containing southern blight germs, and the infection conditions of the crops are recorded and observed.

Results of the experiment

The daily natural bactericide in the market is randomly selected as a control group, then the cure rate and the protection effect of the bactericide are respectively detected under the same detection conditions as those described above, and the results are recorded, so that the following table 1 is obtained, and as can be seen from the table 1, the product prepared in the example 2 has the best cure rate and protection effect.

TABLE 1

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.