阅读说明：本技术 舞花姜在杀螨杀虫方面的用途及其生物农药与制备方法 (Application of wuhuajiang in mite killing and insect killing, biological pesticide and preparation method thereof ) 是由 刘志龙 权美蓉 刘奇志 于 2021-01-12 设计创作，主要内容包括：本发明“舞花姜在杀螨杀虫方面的用途及其生物农药和制备方法”属于生物防治领域。本发明涉及舞花姜在杀螨杀虫方面的用途,以及基于舞花姜精油的生物农药。本发明的生物农药可高效杀灭害螨及仓储害虫,同时无毒无害、安全可靠、成本低廉。(The invention relates to application of alpinia dancing flower in mite killing and insect killing, a biological pesticide and a preparation method thereof, belonging to the field of biological control. The invention relates to application of alpinia dancing flower in mite killing and insect killing, and a biopesticide based on alpinia dancing flower essential oil. The biological pesticide of the invention can efficiently kill harmful mites and storage pests, and is simultaneously nontoxic, harmless, safe, reliable and low in cost.)

1. The application of the litsea cubeba in killing mites and insects is provided.

2. Use of Zingiber dance according to claim 1, wherein the mites are pest mites, the pests are agricultural pests;

preferably, the pest mites are tetranychus urticae;

preferably, the agricultural pest is selected from thrips occidentalis.

3. Use of Zingiber dance according to claim 1 or 2, wherein the acaricidal means is contact or fumigation.

4. Use of Zingiber dance according to any of claims 1-3, characterised in that Zingiber dance essential oil is used to kill mites and insects.

5. Use of Zingiber dance according to claim 4, wherein the Zingiber dance essential oil is extracted from the underground part of Zingiber dance.

6. Application of Zingiber dance in preparing biological pesticide is provided.

7. A biological pesticide is characterized in that the active ingredient of the biological pesticide is the essential oil of the litsea gallows.

8. Biopesticide according to claim 7, characterized in that the biopesticide is selected from acaricides, and/or insecticides;

preferably, the zingiber dancing essential oil is extracted from the underground part of zingiber dancing;

preferably, the active acaricidal ingredients of the zingiber dancing officinale essential oil comprise: camphene, p-methylanisole, 1, 8-cineole, 4-terpineol, alpha-terpineol and bornyl acetate.

9. A preparation method of biological pesticide is characterized in that underground parts of the alpinia dancing.

10. The method for preparing the biological pesticide as claimed in claim 9, wherein the distillation refers to distilling for 8-12h by water boiling to obtain an oil-water mixture;

preferably, the treatment with water is carried out for 8-12h, preferably 10 h;

preferably, the extraction refers to extracting the oil-water mixture with n-hexane;

preferably, the temperature of the water bath for extraction is 38-45 ℃, preferably 40 ℃;

preferably, the mixture obtained by extraction is dried and then n-hexane is removed by evaporation;

preferably, the drying refers to drying with anhydrous sodium sulfate;

preferably, the evaporation refers to evaporation at 40-45 ℃ by using a rotary evaporator.

Technical Field

The invention belongs to the technical field of biological pesticides, and relates to application of alpinia dancing flower in mite killing and insect killing, a biological pesticide and a preparation method thereof.

Background

Tetranychus urticae (Tetranychus urticae) is a worldwide pest mite and belongs to the class of Arachnida. The host plants of the tetranychus urticae are very wide, more than 800 plants of more than 50 families, and the common plants comprise various crops and nearly hundreds of weeds, such as strawberries, eggplants, cucumbers, tomatoes, hot peppers, soybeans, cowpeas, kidney beans, sorghum, watermelons, cucumbers, peaches, pears, apples, apricots, plums, cherries, geraniums, poinsettia, cotton and the like. The two-spotted spider mites and the nymph mites can all be harmed, the initial harmful mites mostly gather on two sides of the vein of the dorsum of the leaves, two sides of the vein of the front of the affected leaves show green loss, the leaves quickly lose green and become brown along with the increase of the number of the mites and the aggravation of the damage degree, the leaves gradually become hard and brittle, and finally become withered and yellow and fall off. The pesticide for preventing and controlling the tetranychus urticae mainly depends on applying a chemosynthetic acaricide, and because of long-term use, the tetranychus urticae has higher resistance to various common acaricides such as abamectin, pyridaben, fenpyroximate, thiamite and the like at present, and the recommended dose is basically ineffective. Meanwhile, the use of chemical agents also has the killing effect on non-target organisms, destroys the ecological balance and causes the pest mites to be rampant. The discovery of high-efficiency and environment-friendly acaricides is urgently needed.

Frankliniella occidentalis (Frankliniella occidentalis) belongs to Thysanoptera thrips, is a worldwide dangerous pest, has wide host range and is a food impurity, host plants comprise more than 500 important economic crops and food crops such as potatoes, eggplants, hot peppers, cucumbers, flowers, bananas, mangoes and the like, and the host types of Frankliniella occidentalis are continuously increased along with the continuous diffusion and spread of Frankliniella occidentalis. The Frankliniella occidentalis eats stems, leaves, flowers and fruits of plants by a rasping-sucking mouthpart, so that petals are discolored, leaves are shrunk, the stems and the fruits form scars, and finally, the plants are withered, and various plant viruses including tomato spotted wilt virus are spread, so that the agricultural production is seriously threatened. The chemical prevention and control method is still a main prevention and control means because the western flower thrips are small in size and secret in harm activities, parthenogenesis can be carried out, the reproductive capacity is strong, the population growth is rapid, but the long-term unreasonable use of chemical pesticides causes that the western flower thrips generate different degrees of drug resistance to insecticides such as organophosphorus, carbamate, pyrethroid and spinosad, so that the prevention and control difficulty is increased, and the pressure on the environment is increased. The discovery of green nuisanceless pesticide effective on frankliniella occidentalis is urgently needed in the production.

The Zingiber dance (Globba racemosa) is a perennial herb of Zingiberaceae, Zingiber, with plant height up to 1 meter; the stem base is enlarged. The long round or oval shape of the blade is in the shape of a needle, the tail tip at the top end, the sharp tip at the base part, the hair thinning or no hair on the two surfaces of the blade, and the margin hair on the tongue and sheath of the blade. The panicle grows, the bracts fall early, the flower is yellow, and each part has an orange gland point; the calyx tube is funnel-shaped, the lobe is reversely folded, the lateral degeneration stamen is in a needle shape, and the length of the lateral degeneration stamen is equal to that of the corolla lobe; the labial flaps are in the shape of an inverted wedge, and no wing-shaped attachments are arranged on two sides of the anther. The capsule is elliptical and blooms in 6-9 months. The wuhua ginger is distributed in india and china, and is mainly distributed in provinces from south to southwest in china. Growing in the shady and wet area with 400-1300 m altitude. The dancing flower ginger is unique in shape and golden in flower color. The full-bloom flowers are like a young girl dancing elegantly and coquettish. Is suitable for indoor pot culture and appreciation or used as a cut flower material. The underground stems of the litsea cubeba are used as the medicine, have pungent and warm natures, and can invigorate the stomach and promote digestion; at present, the alpinia dancing is reported to treat epigastric pain, inappetence and dyspepsia.

However, no report that Zingiber officinale could be used for pest control in the field of biological control has appeared in the field.

Disclosure of Invention

According to the above blank in the art, the present invention provides the use of Zingiber dance in mite-killing and pest-killing, the biopesticide of the present invention is safe and non-toxic to human and animals, environment-friendly, and effective to pest and acarid.

The technical scheme claimed by the invention is as follows:

the application of the litsea cubeba in killing mites and insects is provided.

The mites are pest mites, and the pests are agricultural pests;

preferably, the pest mites are tetranychus urticae;

preferably, the agricultural pest is selected from thrips occidentalis.

The acaricidal and insecticidal mode is contact killing or fumigating killing.

The application of the litsea cubeba in the aspects of killing mites and insects is characterized in that the litsea cubeba essential oil is adopted to kill the mites and the insects;

preferably, the litsea galloprovince essential oil is extracted from the underground part of litsea galloprovince.

Application of Zingiber dance in preparing biological pesticide is provided.

A biological pesticide is characterized in that the active ingredient of the biological pesticide is the essential oil of the litsea gallows.

The biological pesticide is selected from acaricide and/or insecticide;

preferably, the zingiber dancing essential oil is extracted from the underground part of zingiber dancing;

preferably, the active acaricidal ingredients of the zingiber dancing officinale essential oil comprise: camphene, p-methylanisole, 1, 8-cineole, 4-terpineol, alpha-terpineol and bornyl acetate.

A preparation method of biological pesticide is characterized in that underground parts of the alpinia dancing.

The distillation refers to distilling for 8-12h by using a water boiling method to obtain an oil-water mixture;

preferably, the treatment with water is carried out for 8-12h, preferably 10 h;

preferably, the extraction refers to extracting the oil-water mixture with n-hexane;

preferably, the temperature of the water bath for extraction is 38-45 ℃, preferably 40 ℃;

preferably, the mixture obtained by extraction is dried and then n-hexane is removed by evaporation;

preferably, the drying refers to drying with anhydrous sodium sulfate;

preferably, the evaporation refers to evaporation at 40-45 ℃ by using a rotary evaporator.

The invention relates to application of Zingiber gypticum to pest and acarid control.

Application of Zingiber dance in preparing product for preventing and treating pest and mite is provided.

The application of the volatile oil of the litsea henryi in preventing and controlling pests and mites.

The total volatile oil of the alpinia dancing flower is prepared by taking the alpinia dancing flower as a raw material and adopting a water boiling method.

The application of the total volatile oil of the alpinia dancae in the medicament for preventing and treating pests and mites is characterized in that the medicinal component of the medicament is the total volatile oil of the alpinia dancae.

The dancing flower ginger total volatile oil is prepared by adopting a water boiling method.

The boiling method comprises the steps of distillation, extraction, dehydration and solvent evaporation.

The pesticide for preventing and controlling pests and mites is characterized in that the pesticide effect component of the pesticide is the total volatile oil of the alpinia dancae.

The pesticide for preventing and controlling pests and mites is characterized in that the pesticide effect component of the pesticide comprises the total volatile oil of the Zingiber officinale.

A method for controlling pests and mites, which is characterized by comprising the following steps:

the odor substances in the Zingiber dance materials are diffused into the grain storage space to be prevented and controlled,

The total volatile oil of the Zingiber officinale Roscoe is volatilized or fumigated in a grain storage space to be controlled and/or

The Zingiber zerumbet Hance total volatile oil is sprayed on places, grains or grain storage containers where pests and mites are likely to occur.

Can be prepared into products for preventing and controlling the pests and the mites in various forms according to the requirements, and is used for preventing and controlling the pests and the mites in different scales.

Through further research, the total volatile oil extracted by taking the alpinia dancae as the raw material has better effect of preventing and controlling pests and mites. The research of the invention shows that the total volatile oil extracted from the Zingiber gallopauciflorum has obvious contact poisoning and fumigation activity on pests and mites, and can prevent and reduce the damage of the pests on stored grains. The volatile oil of the Zingiber officinale Roscoe is used as a natural insecticidal active substance, the activity of the volatile oil disappears or is decomposed by microorganisms after use, the volatile oil does not pollute grains and the environment, is safe to people and livestock, and can be directly applied to the prevention and control of pests and mites; the total volatile oil of the alpinia dancae is extracted from plants, the active ingredients are complex and diverse, and the active ingredients have different action mechanisms when acting on the target organisms, so that the target organisms are difficult to generate drug resistance on each ingredient, and the lasting control effect is obtained; compared with chemical pesticides, the effective components of the total volatile oil of the Zingiber officinale Roscoe are easy to decompose after killing pests, and cannot accumulate in the bodies of the pests, so that the total volatile oil is not easy to cause harm to other beneficial insects or natural enemies of the pests, and the ecological environment is protected.

In some embodiments of the invention, the total volatile oil of Zingiber dance is prepared by a water boiling method.

In some embodiments of the invention, the poaching process includes distillation, extraction, dehydration, and solvent evaporation steps.

In one embodiment of the invention, the total volatile oil of the alpinia dancing ginger is prepared by the following preparation method: slicing 5000g of Litsea cubeba plant, placing the sliced Litsea cubeba into an improved autoclave, adding water, soaking, distilling with water for 8-12h, extracting the obtained oil-water mixture with n-hexane, dehydrating the extracted product with anhydrous sodium sulfate, evaporating and concentrating in a water bath kettle at 40-45 ℃ under reduced pressure by using a rotary evaporator, and recovering n-hexane to obtain residual oily liquid, namely the total volatile oil of the Litsea cubeba. The preparation method is simple, the cost of the required reagent is low, the mass production is facilitated, and the large-scale prevention and control of pests and mites are facilitated.

In other embodiments of the present invention, the agent for controlling pest mites comprises the total volatile oil of Zingiber officinale.

According to the technical common knowledge of the skilled in the art, the above-mentioned agent for controlling pests and mites of the present invention can also be added with some plant volatile oil or other kinds of chemical substances known to those skilled in the art to have activity of killing pests and mites.

The pesticide for preventing and controlling the pests and the mites can be used for preventing and controlling the pests and the mites in stored grains. Indoor toxicity measurement shows that the volatile oil of the alpinia dancing flower has obvious insecticidal effect on pests and mites. The volatile oil of the Zingiber officinale is the total volatile oily component extracted from Zingiber officinale of Zingiberaceae, and has the advantages of complex effective components, multiple action sites, no drug resistance of pest and pest mites, and long-term effective prevention and treatment effect.

The invention also provides a method for preventing and controlling pests and mites. The method is characterized in that: the method comprises the steps of enabling odor substances in the Zingiber officinale Roscoe materials to be emitted into a grain storage space to be controlled, enabling the Zingiber officinale Roscoe total volatile oil to be volatilized or fumigated in the grain storage space to be controlled, and/or spraying the Zingiber officinale total volatile oil on places, grains or grain storage containers where pests and mites are likely to occur. The method can select a suitable product for preventing and treating the pests and the mites, which is prepared from the Zingiber gypticum Hance, according to the actual factors of the grain storage space, the propagation speed of the pests and the mites, the grain storage environment temperature and the like, and adopts a suitable method for preventing and treating the pests and the mites.

In conclusion, the pesticide for preventing and treating pests and mites belongs to botanical pesticides, is easy to decompose, does not pollute the environment, and is safe to people and livestock; the active ingredients of the volatile oil of the alpinia officinarum maxim have multiple action mechanisms on pests, are not easy to cause the pests to generate drug resistance, and can be used for a long time; the pesticide loses activity after being killed, cannot be accumulated in the body of pests, and protects the ecological environment; the preparation method is simple and easy to implement, the wild plants of the Zingiber dance flower are widely distributed, the raw materials are easy to obtain, the cost is low, and the method is suitable for large-scale production and can be widely used for preventing and treating pests and mites.

The total volatile oil of the dancing flower ginger: the invention also called as the "Zingiber dance" essential oil refers to the volatile oil mixture extracted by the conventional method for extracting the total volatile oil of plants, which is commonly used in the field, by using Zingiber dance as a raw material, and the components of the volatile oil mixture are different according to the extraction process adopted by the person skilled in the art.

The invention also claims application of the Zingiber officinale to pest and mite control.

The invention provides application of Zingiber officinale to prevention and control of pests and mites, and the products in various forms can be used for prevention and control of the pests and mites by various methods according to actual needs. The following description is by way of example only and is not intended as a limitation on the use of Zingiber gypticum for controlling pest mites. The skilled person can implement various possible applications according to the technical solutions of the present invention and the knowledge of the prior art.

The use of Zingiber dance in the control of pest mites is illustrated below:

1. when the stored grain is not harmed by pests and mites: the medicament made of the dancing flower ginger can be solid or semisolid, and the medicament is placed at the four corners and the middle part of the grain storage space (the medicament is evenly placed according to the actual size of the grain storage space), so that odor substances in the dancing flower ginger material are slowly diffused into the grain storage space to be prevented and treated, and the grain storage space is protected for a long time.

2. When the stored grain is damaged by pests and mites: the liquid preparation prepared from the litsea henryi can be adopted, and the method of natural volatilization, artificial fumigation or spraying is adopted according to the damage of pests, so that the total volatile oil of the litsea henryi is volatilized into the whole grain storage space, the pests and mites are quickly killed, and the damage is reduced to the minimum degree; after the pest and mite are completely killed, the solid or semisolid medicament prepared from the litsea henryi is replaced, and the secondary harm of the pest is prevented for a long time.

3. When the environmental temperature of grain storage is low: the liquid preparation prepared from the litsea cubeba can be adopted, and the natural volatilization, artificial fumigation or spraying method is adopted, so that the total volatile oil of the litsea cubeba is volatilized into a grain storage space, and the pest and acarid can be prevented.

4. When the environmental temperature of grain storage is high: the preparation can be made of Zingiber dance flower in solid or semisolid dosage form, and is uniformly distributed in the space for storing grain to prevent and treat pest and acarid.

Detailed Description

The present invention is described below with reference to specific examples, which are intended to be illustrative and illustrative only and are not to be construed as limiting the scope of the present invention.

Sources and access routes of test materials

The frankliniella occidentalis and the tetranychus urticae are both bred in the laboratory of the applicant, and are both common insects/mites which are easily obtained in nature.

The wuhua ginger was collected from yunnan baoshan in 2020 and 10 months.

Dancing flower ginger essential oil: the method is suitable for leaching the medicinal material components which have volatility, can be distilled along with water vapor without being damaged, and are stable in water and insoluble or insoluble in water.

The method for preparing the zingiber dancing officinale essential oil is as in experimental example 1. The experimental reagents which are not particularly described in the invention are all conventional reagents in the field, or are prepared by adopting conventional methods in the field, can be obtained commercially, and have the specification of laboratory pure grade.

Group 1 example, New use of Litsea Coreana for biological control

The group of embodiments provides uses of alpinia dancing in killing mites and insects.

In particular embodiments, the mites are pest mites, and the pests are agricultural pests;

preferably, the pest mites are tetranychus urticae;

preferably, the agricultural pest is selected from thrips occidentalis.

In some embodiments, the control refers to killing;

in other embodiments, the killing mode is contact killing or fumigation.

In a further embodiment, the Zingiber dance flower essential oil is used for killing mites and insects.

Preferably, the litsea galloprovince essential oil is extracted from the underground part of litsea galloprovince.

Group 2 examples biocontrol pharmaceutical use of Zingiber dance

The present group of embodiments provides the use of Zingiber dance in the preparation of biopesticides.

In particular embodiments, the biopesticides include, but are not limited to: insecticides, acaricides.

More specifically, the biopesticide may be formulated into conventional dosage forms in the art, including, but not limited to: powders, wettable powders, drops, suspension concentrates, emulsifiable concentrates, sprays, aerosols, baits, mosquito-repellent incense, sticking agents, smoke agents, insecticidal coatings and the like.

Group 3 examples of biopesticides of the present invention

The present group of embodiments provides a biopesticide. All embodiments of this group share the following common features: the active ingredient of the biological pesticide is the litsea gallows ginger essential oil.

In particular embodiments, the biopesticide is in a dosage form selected from the group consisting of an acaricide or an insecticide;

preferably, the active ingredients of the zingiber dancing oil comprise: the following ingredients in table 1 were identified as insecticidal active ingredients: camphene, p-methylanisole, 1, 8-cineole, 4-terpineol, alpha-terpineol and bornyl acetate.

In further embodiments, the biopesticide further comprises a pesticide art-acceptable adjuvant common in the art, including, but not limited to: emulsifiers, organic solvents, fillers, wetting agents, diluents, and the like.

Group 4 examples, methods of preparing biopesticides of the present invention

The present group of embodiments provides a method for preparing a biopesticide. All embodiments of this group share the following common features: the preparation method comprises the following steps: decocting rhizoma Drynariae in water, and extracting.

In a specific embodiment, the water boiling method is used for treating for 8-12h to obtain an oil-water mixture;

preferably, the extraction refers to extracting the oil-water mixture obtained by distillation with n-hexane;

preferably, the temperature of the water bath for extraction is 35-40 ℃, preferably 40 ℃;

preferably, the Zingiber dance is sliced before the water boiling;

preferably, the underground part of the alpinia dancing is sliced and boiled for 8-12 h.

Preferably, the mixture obtained by extraction is dried and then n-hexane is removed by evaporation;

preferably, the drying refers to drying with anhydrous sodium sulfate;

preferably, the evaporation refers to evaporation at 40-45 ℃ by using a rotary evaporator.

Experimental example 1 preparation method of Zingiber dance ginger essential oil

In the present invention, although a method for extracting zingiber dancaefolia hance essential oil is provided, the method is not limited to the method for extracting zingiber dancaefolia hance essential oil for achieving the object of the present invention.

Slicing 5000g of underground part of fresh Zingiber dance, extracting oil-water mixture obtained by water boiling method with n-hexane, dehydrating the extract with anhydrous sodium sulfate, evaporating and concentrating in a water bath kettle at 40-45 deg.C under reduced pressure with a rotary evaporator, recovering n-hexane, and collecting the residual oily liquid, i.e. Zingiber dance volatile oil.

Experimental example 2 GC-MS detection of essential oil of Helianthus dance

The Zingiber dancing flower essential oil prepared in example 1 is sent to the GC center of Beijing university.

An Agilent6890N gas chromatograph and an Agilent5973N mass spectrometer were used.

HP-5MS30mm × 0.25mm × 0.25 μm quartz capillary column, initial column temperature 60 deg.C maintained for 1 min, programmed temperature up to 180 deg.C at a rate of 12 deg.C/min maintained for 0.5 min, programmed temperature up to 280 deg.C at a rate of 25 deg.C/min maintained for 18 min, vaporizer temperature 280 deg.C, carrier gas helium, column flow 1.0mL/min, split ratio 1: 10.

The ionization mode EI of the mass spectrum has the electron bombardment energy of 70eV, the scanning mass range of 20-550m/z and the scanning time of 2 seconds.

Sampling 1 mu L of the litsea henryi plant essential oil (500 times of n-hexane diluent), analyzing and identifying by using a gas chromatography-mass spectrometer, retrieving a Nist05 standard mass spectrum library by using an HP-MSD chemical workstation, and detecting and identifying 30 peaks by combining related documents. The results of the identification are shown in table 1 below:

TABLE 1 chemical composition of essential oil of Zingiber dance

The total percentage of all the components identified in Table 1 above is less than 100% because there are unknown components in the essential oil of Zingiber dance, which may be new compounds and cannot be identified by the existing GC-MS detection method.

Experimental example 3 indoor toxicity determination of Zingiber gypti essential oil on pest mites

The killing effect of the ginger dancing essential oil of the invention on tetranychus urticae is carried out by adopting a leaf disc methodFruit verification, the specific operation is as follows: coating polytetrafluoroethylene on the inner wall of the culture plate, and drying by electric air blowing; heating water and agar at a ratio of 100:1.5 in a microwave oven, mixing, pouring into 12-well cell culture plate (2-3 ml per well), and cooling to solidify; taking fresh kidney bean leaves without pesticide, and punching the leaves by using a puncher with the diameter of 19mm to prepare a circular leaf disc; dissolving a certain amount of Zingiber dance flower essential oil with anhydrous ethanol (the amount is not more than 2% of the liquid medicine system), adding dimethyl sulfoxide (not more than 1% of the liquid medicine system) for assisting dissolution, preparing a mother solution, and diluting with distilled water to a required concentration to obtain the liquid medicine system; soaking the leaf disc in the agent for 10s, taking out, air drying, placing the kidney bean leaves soaked with the liquid medicine on a culture plate with agar poured therein with the back side of the kidney bean leaves upward, and inoculating 10 tetranychus urticae into each hole to form mites; firmly sticking the porous surface of the culture plate by rice paper, and putting the culture plate into an incubator at the temperature of 26-28 ℃, the relative humidity of 65-75% and the photoperiod L: D of 16: 8; observing and recording death number under the stereoscope after 24h, determining that the person who touches with the brush pen is alive and the person who does not touch is dead, and calculating LD₅₀。

Mortality (%) ═ death/test insect count 100

Corrected mortality (%) - (treatment-control mortality)/(1-control mortality) × 100

semi-Lethal Dose (LD)₅₀): refers to the dose required to cause death in 50% of the subjects tested. Calculated by using Probit software.

The results are shown in table 2 below:

table 2, lethal test data of the Zingiber dance essential oil of the present invention on Tetranychus urticae

Frankliniella occidentalis lethal experiment: dissolving a certain amount of Zingiber dance ginger essential oil with anhydrous ethanol (the amount is not more than 1% of the liquid medicine system), adding Tween 80 (not more than 0.5% of the liquid medicine system) for assisting dissolution, preparing a mother solution, and diluting with distilled water to a required concentration to obtain the liquid medicine system for later use; putting 1mL of liquid medicine into a glass scintillation bottle with the volume of 22mL, uniformly rolling for a plurality of circles to form a medicine film, pouring out the liquid medicine, and airing the scintillation bottle for later use. Taking fresh hyacinth beans without pesticide, preparing the hyacinth beans into a long section of 1cm square, immersing the hyacinth beans into the pesticide for 10s, taking out the hyacinth beans, airing the hyacinth beans, and placing the hyacinth beans in a scintillation bottle; setting 5 repetitions for each treatment, repeatedly inoculating second-instar nymphs of 10 Frankliniella occidentalis, and sealing the bottle mouth with a 150-mesh insect-proof net; and (5) placing the worm body at room temperature, observing the result after 24h, and stirring the worm body with a writing brush, wherein the worm body is recorded as dead after being immobilized.

Mortality (%) ═ death/test insect count 100

Corrected mortality (%) - (treatment-control mortality)/(1-control mortality) × 100

semi-Lethal Dose (LD)₅₀): refers to the dose required to cause death in 50% of the subjects tested. Calculated by using Probit software.

The results are shown in Table 3:

TABLE 3 lethality test data of the essential oil of Zingiber dance of the present invention against Frankliniella occidentalis

The meanings of the semi-lethal dose LC50 in table 2-3 above are: within 24 hours, the death rate of the tested individuals can reach 50% by treating the test individuals with the concentration of the dancing ginger essential oil contained in each milliliter of the liquid medicine system (taking alcohol, dimethyl sulfoxide and distilled water as solvents).

It is known in the art that only 5 to 6 effective concentrations are required in toxicological tests, and at this concentration, the individual having the insect die, and the concentrations at which the mortality rate of 100% and 0% cannot be counted as effective concentrations, so that the half-lethal dose for killing mites and insects was determined in this experimental example based on 5 series of concentrations (each concentration is set to 5 replicates) listed in tables 2 to 3, respectively.