阅读说明：本技术 一种基于益生菌的鳗鱼无抗饲料及其制备方法 (Probiotic-based antibiotic-free eel feed and preparation method thereof ) 是由 何淑玲 孙耶宾 余建芳 马令法 杨敬军 常毓巍 于 2021-01-20 设计创作，主要内容包括：本发明提供一种基于益生菌的鳗鱼无抗饲料,包括以下组分,以重量份计算：鱼粉30-45份；淀粉30-45份；维生素5-10份；矿物质混合物1-5份；中药提取剂10-30份；双歧杆菌1-3份；乳酸菌1-5份；大豆异黄酮2-15份；其中中药提取剂包括以下组分,以重量份计算：葛根5-10份、黄芪7-15份、鸡血藤10-25份、陈皮12-30份、肉桂5-20份、柠檬汁7-15份、蜂蜜8-15份、鸡内金3-8份、西红花4-17份、辛夷7-24份。本发明通过设置鱼粉、淀粉、维生素、矿物质混合物、中药提取剂、双歧杆菌、乳酸菌和大豆异黄酮等成分,为鳗鱼的生长提供有利的营养物质,双歧杆菌、乳酸菌等为鳗鱼提供益生菌,便于鳗鱼消化吸收上述营养物质,上述组分互相相成协同作用为鳗鱼更佳的营养。(The invention provides probiotics-based eel antibiotic-free feed which comprises the following components in parts by weight: 30-45 parts of fish meal; 30-45 parts of starch; 5-10 parts of vitamin; 1-5 parts of a mineral mixture; 10-30 parts of a traditional Chinese medicine extracting agent; 1-3 parts of bifidobacterium; 1-5 parts of lactic acid bacteria; 2-15 parts of soybean isoflavone; the traditional Chinese medicine extractant comprises the following components in parts by weight: 5-10 parts of kudzuvine root, 7-15 parts of astragalus root, 10-25 parts of suberect spatholobus stem, 12-30 parts of tangerine peel, 5-20 parts of cassia bark, 7-15 parts of lemon juice, 8-15 parts of honey, 3-8 parts of chicken's gizzard-membrane, 4-17 parts of saffron and 7-24 parts of biond magnolia flower. The invention provides beneficial nutrient substances for the growth of eels by arranging fish meal, starch, vitamins, mineral mixture, traditional Chinese medicine extractant, bifidobacteria, lactic acid bacteria, soybean isoflavone and other components, provides probiotics for eels, facilitates the eels to digest and absorb the nutrient substances, and the components mutually form synergistic action to provide better nutrition for eels.)

1. The probiotics-based eel antibiotic-free feed is characterized by comprising the following components in parts by weight:

30-45 parts of fish meal; 30-45 parts of starch; 5-10 parts of vitamin; 1-5 parts of a mineral mixture; 10-30 parts of a traditional Chinese medicine extracting agent; 1-3 parts of bifidobacterium; 1-5 parts of lactic acid bacteria; 2-15 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

5-10 parts of kudzuvine root, 7-15 parts of astragalus root, 10-25 parts of suberect spatholobus stem, 12-30 parts of tangerine peel, 5-20 parts of cassia bark, 7-15 parts of lemon juice, 8-15 parts of honey, 3-8 parts of chicken's gizzard-membrane, 4-17 parts of saffron and 7-24 parts of biond magnolia flower.

2. The probiotic-based eel antibiotic-free feed according to claim 1,

the adhesive comprises the following components in parts by weight:

35-40 parts of fish meal; 32-38 parts of starch; 6-8 parts of vitamin; 2-4 parts of a mineral mixture; 15-25 parts of a traditional Chinese medicine extracting agent; 2-3 parts of bifidobacterium; 3-4 parts of lactic acid bacteria; 5-10 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

7-9 parts of kudzu root, 9-10 parts of astragalus, 15-20 parts of suberect spatholobus stem, 15-26 parts of dried orange peel, 10-15 parts of cinnamon, 9-12 parts of lemon juice, 10-13 parts of honey, 5-7 parts of chicken's gizzard-membrane, 8-15 parts of saffron and 10-18 parts of biond magnolia flower.

3. The probiotic-based eel antibiotic-free feed according to claim 2,

the adhesive comprises the following components in parts by weight:

37 parts of fish meal; 35 parts of starch; 7 parts of vitamin; 3 parts of a mineral mixture; 18 parts of a traditional Chinese medicine extracting agent; 2 parts of bifidobacterium; 3 parts of lactic acid bacteria; 8 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

8 parts of kudzuvine root, 10 parts of astragalus root, 18 parts of suberect spatholobus stem, 20 parts of tangerine peel, 13 parts of cassia bark, 10 parts of lemon juice, 12 parts of honey, 6 parts of chicken's gizzard-membrane, 10 parts of saffron and 15 parts of biond magnolia flower.

4. The method for preparing probiotic bacteria-based eel antibiotic-free feed according to any one of claims 1 to 3, comprising the steps of:

s1: weighing 30-45 parts of fish meal; 30-45 parts of starch; 5-10 parts of vitamin; 1-5 parts of a mineral mixture; 10-30 parts of a traditional Chinese medicine extracting agent; 1-3 parts of bifidobacterium; 1-5 parts of lactic acid bacteria; 2-15 parts of soybean isoflavone;

s2: fully mixing the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and forming agent into the materials, dissolving completely to obtain paste, adding into a granulator to obtain round granules, and drying with a dryer;

s5: and (5) after the round particles obtained in the step (S4) are molded, spraying a layer of water mist on the surfaces of the round particles to enable the surfaces of the round particles to be slightly wet, adding the powder obtained in the step (S2) into the round particles to enable the powder to be uniformly coated on the outer surfaces of the round particles, and thus obtaining the antibiotic-free feed.

5. The method for preparing probiotic bacteria-based feed for eel having no antibiotic according to claim 4,

in the step S4: the temperature of the dryer is set to be 20-40 ℃, and the drying time is 12-48 hours.

6. The method for preparing probiotic bacteria-based feed for eel having no antibiotic according to claim 4,

in the step S5:

dividing the powder into 3 equal parts, adding the first equal part into the round particles to coat a layer of powder on the surfaces of the round particles, and after the first layer of powder is coated, drying the powder in a dryer, wherein the temperature of the dryer is set to be 30-45 ℃, and the drying time is 2-6 hours;

after the first layer of powder is dried, spraying a layer of water mist on the surface of the first layer of powder, then adding a second equal part of powder, uniformly wrapping the second equal part of powder on the surface of the first layer of powder, and drying in a dryer, wherein the drying setting temperature is 25-35 ℃, and the drying time is 2-6 hours;

after the second layer of powder is dried, spraying a layer of water mist on the surface of the third layer of powder, then adding a third equal part of powder, uniformly coating the third equal part of powder on the surface of the second layer of powder, and drying in a dryer at the set temperature of 30-45 ℃ for 2-6 hours.

7. The method for preparing probiotic bacteria-based eel antibiotic-free feed according to claim 5 or 6, wherein the method for preparing the traditional Chinese medicine extractant comprises the following steps:

s1: weighing; 5-10 parts of kudzuvine root, 7-15 parts of astragalus root, 10-25 parts of suberect spatholobus stem, 12-30 parts of dried orange peel, 5-20 parts of cinnamon, 7-15 parts of lemon juice, 8-15 parts of honey, 3-8 parts of chicken's gizzard-membrane, 4-17 parts of saffron and 7-24 parts of biond magnolia flower;

s2: adding radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae into a rectifying tower, rectifying for 4-10 hr, and collecting distillate;

s3: adding the rectification liquid obtained in the step S2 into a concentration tank for concentration to obtain a concentrated material;

s4: step S3, after the concentrated material is obtained, adding lemon juice and honey into the concentrated material, and uniformly stirring to obtain a traditional Chinese medicine ointment;

s5: transferring the Chinese medicinal paste obtained in step S4 into a dryer, and drying to obtain powdered Chinese medicinal extract.

8. The eel antibiotic-free feed prepared by the method for preparing eel antibiotic-free feed based on probiotics according to claim 7.

Technical Field

The invention relates to the technical field of fish, and particularly relates to probiotics-based eel antibiotic-free feed and a preparation method thereof.

Background

Eel, also called eel, is a fish with a snake-like appearance, has the basic characteristics of fish, but has no scales, and is generally produced in a salt-water boundary sea area. Eel is delicious in meat quality, is rich in high-quality protein, amino acid, calcium, DHA, EPA and multiple vitamins, has high edible and nutritional values, is always favored by people, has 18 varieties in the whole world, is common in Japanese eel, American eel, weever and the like, is mainly bred by the Japanese eel and the American eel in Chinese eel, is mainly concentrated in coastal provinces such as Guangdong, Fujian, Zhejiang, Jiangsu and the like, has the total yield accounting for 70 percent of the total global yield, and is the biggest eel breeding producing country and eel product raw material supplying country in the world at present.

In the eel cultivation process, the eel feed is scattered into the cultivation pond, the existing eel feed is rich in nutrition, can provide nutrition for growth and development of eels, but is easy to cause overnutrition, finally, eels are easy to get ill or die in the cultivation process, and accordingly, great economic loss is brought to farmers.

Disclosure of Invention

The invention aims to provide probiotics-based eel antibiotic-free feed and a preparation method thereof, which are used for providing rich nutrition for eels, promoting digestion and absorption for eels and achieving the technical effect of reducing overnutrition.

The invention is realized by the following technical scheme:

the adhesive comprises the following components in parts by weight:

30-45 parts of fish meal; 30-45 parts of starch; 5-10 parts of vitamin; 1-5 parts of a mineral mixture; 10-30 parts of a traditional Chinese medicine extracting agent; 1-3 parts of bifidobacterium; 1-5 parts of lactic acid bacteria; 2-15 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

5-10 parts of kudzuvine root, 7-15 parts of astragalus root, 10-25 parts of suberect spatholobus stem, 12-30 parts of tangerine peel, 5-20 parts of cassia bark, 7-15 parts of lemon juice, 8-15 parts of honey, 3-8 parts of chicken's gizzard-membrane, 4-17 parts of saffron and 7-24 parts of biond magnolia flower.

In order to better implement the present invention, further,

the adhesive comprises the following components in parts by weight:

35-40 parts of fish meal; 32-38 parts of starch; 6-8 parts of vitamin; 2-4 parts of a mineral mixture; 15-25 parts of a traditional Chinese medicine extracting agent; 2-3 parts of bifidobacterium; 3-4 parts of lactic acid bacteria; 5-10 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

7-9 parts of kudzu root, 9-10 parts of astragalus, 15-20 parts of suberect spatholobus stem, 15-26 parts of dried orange peel, 10-15 parts of cinnamon, 9-12 parts of lemon juice, 10-13 parts of honey, 5-7 parts of chicken's gizzard-membrane, 8-15 parts of saffron and 10-18 parts of biond magnolia flower.

In order to better implement the present invention, further,

the adhesive comprises the following components in parts by weight:

37 parts of fish meal; 35 parts of starch; 7 parts of vitamin; 3 parts of a mineral mixture; 18 parts of a traditional Chinese medicine extracting agent; 2 parts of bifidobacterium; 3 parts of lactic acid bacteria; 8 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

8 parts of kudzuvine root, 10 parts of astragalus root, 18 parts of suberect spatholobus stem, 20 parts of tangerine peel, 13 parts of cassia bark, 10 parts of lemon juice, 12 parts of honey, 6 parts of chicken's gizzard-membrane, 10 parts of saffron and 15 parts of biond magnolia flower.

A preparation method of probiotics-based eel antibiotic-free feed comprises the following steps:

s1: weighing 30-45 parts of fish meal; 30-45 parts of starch; 5-10 parts of vitamin; 1-5 parts of a mineral mixture; 10-30 parts of a traditional Chinese medicine extracting agent; 1-3 parts of bifidobacterium; 1-5 parts of lactic acid bacteria; 2-15 parts of soybean isoflavone;

s2: fully mixing the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and forming agent into the materials, dissolving completely to obtain paste, adding into a granulator to obtain round granules, and drying with a dryer;

s5: and (5) after the round particles obtained in the step (S4) are molded, spraying a layer of water mist on the surfaces of the round particles to enable the surfaces of the round particles to be slightly wet, adding the powder obtained in the step (S2) into the round particles to enable the powder to be uniformly coated on the outer surfaces of the round particles, and thus obtaining the antibiotic-free feed.

In order to better implement the present invention, further, in step S4: the temperature of the dryer is set to be 20-40 ℃, and the drying time is 12-48 hours.

In order to better implement the present invention, further, in step S5:

dividing the powder into 3 equal parts, adding the first equal part into the round particles to coat a layer of powder on the surfaces of the round particles, and after the first layer of powder is coated, drying the powder in a dryer, wherein the temperature of the dryer is set to be 30-45 ℃, and the drying time is 2-6 hours;

after the first layer of powder is dried, spraying a layer of water mist on the surface of the first layer of powder, then adding a second equal part of powder, uniformly wrapping the second equal part of powder on the surface of the first layer of powder, and drying in a dryer, wherein the drying setting temperature is 25-35 ℃, and the drying time is 2-6 hours;

after the second layer of powder is dried, spraying a layer of water mist on the surface of the third layer of powder, then adding a third equal part of powder, uniformly coating the third equal part of powder on the surface of the second layer of powder, and drying in a dryer at the set temperature of 30-45 ℃ for 2-6 hours.

In order to better realize the invention, further, the preparation method of the traditional Chinese medicine extractant comprises the following steps:

s1: weighing; 5-10 parts of kudzuvine root, 7-15 parts of astragalus root, 10-25 parts of suberect spatholobus stem, 12-30 parts of dried orange peel, 5-20 parts of cinnamon, 7-15 parts of lemon juice, 8-15 parts of honey, 3-8 parts of chicken's gizzard-membrane, 4-17 parts of saffron and 7-24 parts of biond magnolia flower;

s2: adding radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae into a rectifying tower, rectifying for 4-10 hr, and collecting distillate;

s3: adding the rectification liquid obtained in the step S2 into a concentration tank for concentration to obtain a concentrated material;

s4: step S3, after the concentrated material is obtained, adding lemon juice and honey into the concentrated material, and uniformly stirring to obtain a traditional Chinese medicine ointment;

s5: transferring the Chinese medicinal paste obtained in step S4 into a dryer, and drying to obtain powdered Chinese medicinal extract.

The action mechanism is as follows:

the invention provides probiotics-based eel antibiotic-free feed which mainly comprises fish meal, starch, vitamins, mineral mixtures, a traditional Chinese medicine extracting agent, bifidobacteria, lactic acid bacteria and soybean isoflavone and provides rich nutrient substances for eel growth, wherein the bifidobacteria and the lactic acid bacteria provide beneficial bacteria for the eel, so that the eel can promote the growth and development of the eel in the growth process, and the digestion and absorption effects of the eel are better.

In order to promote the eel to digest and absorb in the growth process and reduce the incidence probability, a traditional Chinese medicine extractant is arranged in the components of the eel, the traditional Chinese medicine extractant mainly comprises kudzu root, astragalus, suberect spatholobus stem, tangerine peel, cinnamon, lemon juice, honey, chicken's gizzard-membrane, saffron and biond magnolia flower, the traditional Chinese medicine extractant contains various substances, can enhance the resistance of the eel in the growth environment and reduce the incidence probability, and is matched with components such as bifidobacterium, lactobacillus and the like in a synergistic way in the whole formula, so that the nonreactive feed which is more beneficial to the growth of the eel is obtained.

In the common method for preparing the eel feed, a plurality of components are ground and crushed together and then stirred and mixed to form the eel feed, the eel feed prepared by the common method is very easy to disperse in water, and after dispersion, the eel has smaller effect of absorbing favorable components, besides, the eel feed has certain pollution to the growth environment of the eel and is also very unfavorable for the growth of the eel.

The inventor fully researches eel feed to obtain a set of preparation method which is beneficial to eel growth and convenient to absorb, and specifically comprises the steps of fully mixing a mixture of bifidobacterium, lactobacillus, vitamins and mineral substances, adding water and a forming agent to enable the mixture to be micro-particles, manufacturing the particles by a granulator, preparing the particles into round particles, and drying the round particles by a dryer to enable the shape and the size of the round particles to be uniform, so that the follow-up eel can conveniently eat.

In addition, the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone are prepared into powder, and the powder is wrapped on the surface of the round particle.

Before each coating, a layer of water mist is sprayed on the surface of the round particles to moisten the surfaces of the round particles, so that the powder is adsorbed and then dried by a dryer, and the round particles are coated layer by layer, so that the antibiotic-free feed prepared after coating can be more favorable for eel eating, is not easy to disperse in water, and has relatively slow dispersion speed in water even if dispersed, and is favorable for keeping the growth environment of eels clean.

In addition to the above, the eel obtained by layer-by-layer wrapping has no antibiotic feed, after the eel inhales, firstly, the eel absorbs nutrient substances such as fish meal at the outer layer, and finally, the eel absorbs substances such as probiotics, so that the absorption sequence is more favorable for the growth of the eel, the eel is gradually digested, the possibility of overnutrition is reduced, and as the traditional Chinese medicine extractant is added into the components, the resistance of the eel is further promoted, and the eel grows more stably.

The invention has the beneficial effects that:

the invention provides beneficial nutrient substances for the growth of eels by arranging fish meal, starch, vitamins, mineral mixture, traditional Chinese medicine extractant, bifidobacteria, lactic acid bacteria, soybean isoflavone and other components, provides probiotics for eels, facilitates the eels to digest and absorb the nutrient substances, and the components mutually form synergistic action to provide better nutrition for eels.

The invention also provides a preparation method of the eel nonreactive feed based on the probiotics, which is characterized in that the round particles are prepared by preferentially mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the mineral substances, then fish meal, the traditional Chinese medicine extractant, starch, soybean isoflavone and other components are mixed into powder, the powder is wrapped on the surfaces of the round particles in batches, so as to prepare the eel nonreactive feed based on the probiotics, the feed is not easy to disperse in water and is beneficial to maintaining the eel breeding environment, in addition, the feed is more in line with the processes of eel absorption and digestion and the like, so that the eel growth is more beneficial, the traditional Chinese medicine extractant is added so as to provide better resistance for the eel, the morbidity and the like, so as to set the bifidobacteria, the lactic acid bacteria and other components, thereby helping the eel to digest and absorb, reducing the morbidity and the like due to overfeeding, and the eel feed finally prepared by the components and the preparation method in a synergistic way, provides better support for the growth of the eel, and is not necessary.

Detailed Description

The technical solution of the present invention will be described below with reference to the embodiments of the present invention.

Example 1:

a probiotics-based antibiotic-free eel feed,

the adhesive comprises the following components in parts by weight:

30 parts of fish meal; 30 parts of starch; 5 parts of vitamin; 1 part of mineral mixture; 10 parts of a traditional Chinese medicine extracting agent; 1 part of bifidobacterium; 1 part of lactic acid bacteria; 2 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

5 parts of kudzuvine root, 7 parts of astragalus root, 10 parts of suberect spatholobus stem, 12 parts of tangerine peel, 5 parts of cassia bark, 7 parts of lemon juice, 8 parts of honey, 3 parts of chicken's gizzard-membrane, 4 parts of saffron and 7 parts of biond magnolia flower.

A preparation method of probiotics-based eel antibiotic-free feed comprises the following steps:

s1: weighing the following components in parts by weight, namely fish meal; starch; a vitamin; a mineral mixture; a Chinese medicine extracting agent; a bifidobacterium; lactic acid bacteria; soy isoflavones;

s2: fully mixing the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and a forming agent into the materials, fully dissolving the materials into paste, adding the paste into a granulator to prepare round granules, and drying the round granules by using a dryer, wherein the temperature of the dryer is set to be 20 ℃, and the drying time is 12 hours;

s5: dividing the powder into 3 equal parts, adding the first equal part into the round particles to coat a layer of powder on the surfaces of the round particles, and after the first layer of powder is coated, drying the powder in a dryer, wherein the temperature of the dryer is set to be 30-45 ℃, and the drying time is 2-6 hours;

after the first layer of powder is dried, spraying a layer of water mist on the surface of the first layer of powder, then adding a second equal part of powder, uniformly wrapping the second equal part of powder on the surface of the first layer of powder, and drying in a dryer, wherein the drying setting temperature is 25-35 ℃, and the drying time is 2-6 hours;

after the second layer of powder is dried, spraying a layer of water mist on the surface of the third layer of powder, then adding a third equal part of powder, uniformly coating the third equal part of powder on the surface of the second layer of powder, and drying in a dryer at the set temperature of 30-45 ℃ for 2-6 hours.

The preparation method of the traditional Chinese medicine extractant comprises the following steps:

s1: weighing; kudzu root, astragalus root, spatholobus stem, dried orange peel, cinnamon, lemon juice, honey, chicken's gizzard-membrane, saffron and magnolia flower;

s2: adding radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae into a rectifying tower, rectifying for 4-10 hr, and collecting distillate;

s3: adding the rectification liquid obtained in the step S2 into a concentration tank for concentration to obtain a concentrated material;

s4: step S3, after the concentrated material is obtained, adding lemon juice and honey into the concentrated material, and uniformly stirring to obtain a traditional Chinese medicine ointment;

s5: transferring the Chinese medicinal paste obtained in step S4 into a dryer, and drying to obtain powdered Chinese medicinal extract.

Example 2:

a probiotics-based antibiotic-free eel feed,

the adhesive comprises the following components in parts by weight:

45 parts of fish meal; 45 parts of starch; 10 parts of vitamin; 5 parts of a mineral mixture; 30 parts of a traditional Chinese medicine extracting agent; 3 parts of bifidobacterium; 5 parts of lactic acid bacteria; 15 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

10 parts of kudzuvine root, 15 parts of astragalus root, 25 parts of suberect spatholobus stem, 30 parts of tangerine peel, 20 parts of cassia bark, 15 parts of lemon juice, 15 parts of honey, 8 parts of chicken's gizzard-membrane, 17 parts of saffron and 24 parts of biond magnolia flower.

A preparation method of probiotics-based eel antibiotic-free feed comprises the following steps:

s1: weighing the following components in parts by weight, namely fish meal; starch; a vitamin; a mineral mixture; a Chinese medicine extracting agent; a bifidobacterium; lactic acid bacteria; soy isoflavones;

s2: fully mixing the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and a forming agent into the materials, fully dissolving the materials into paste, adding the paste into a granulator to prepare round granules, and drying the round granules by using a dryer, wherein the temperature of the dryer is set to be 40 ℃, and the drying time is 48 hours;

s5: dividing the powder into 3 equal parts, adding the first equal part into the round particles to coat a layer of powder on the surfaces of the round particles, and after the first layer of powder is coated, drying the powder in a dryer, wherein the temperature of the dryer is set to be 30-45 ℃, and the drying time is 2-6 hours;

after the first layer of powder is dried, spraying a layer of water mist on the surface of the first layer of powder, then adding a second equal part of powder, uniformly wrapping the second equal part of powder on the surface of the first layer of powder, and drying in a dryer, wherein the drying setting temperature is 25-35 ℃, and the drying time is 2-6 hours;

after the second layer of powder is dried, spraying a layer of water mist on the surface of the third layer of powder, then adding a third equal part of powder, uniformly coating the third equal part of powder on the surface of the second layer of powder, and drying in a dryer at the set temperature of 30-45 ℃ for 2-6 hours.

The preparation method of the traditional Chinese medicine extractant comprises the following steps:

s1: weighing; radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, lemon juice, Mel, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae;

s2: adding radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae into a rectifying tower, rectifying for 4-10 hr, and collecting distillate;

s3: adding the rectification liquid obtained in the step S2 into a concentration tank for concentration to obtain a concentrated material;

s4: step S3, after the concentrated material is obtained, adding lemon juice and honey into the concentrated material, and uniformly stirring to obtain a traditional Chinese medicine ointment;

s5: transferring the Chinese medicinal paste obtained in step S4 into a dryer, and drying to obtain powdered Chinese medicinal extract.

Example 3:

a probiotics-based antibiotic-free eel feed,

the adhesive comprises the following components in parts by weight:

37 parts of fish meal; 35 parts of starch; 7 parts of vitamin; 3 parts of a mineral mixture; 18 parts of a traditional Chinese medicine extracting agent; 2 parts of bifidobacterium; 3 parts of lactic acid bacteria; 8 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

8 parts of kudzuvine root, 10 parts of astragalus root, 18 parts of suberect spatholobus stem, 20 parts of tangerine peel, 13 parts of cassia bark, 10 parts of lemon juice, 12 parts of honey, 6 parts of chicken's gizzard-membrane, 10 parts of saffron and 15 parts of biond magnolia flower.

A preparation method of probiotics-based eel antibiotic-free feed comprises the following steps:

s1: weighing the following components in parts by weight, namely fish meal; starch; a vitamin; a mineral mixture; a Chinese medicine extracting agent; a bifidobacterium; lactic acid bacteria; soy isoflavones;

s2: fully mixing the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and a forming agent into the materials, fully dissolving the materials into paste, adding the paste into a granulator to prepare round granules, and drying the round granules by using a dryer, wherein the set temperature of the dryer is 28 ℃, and the drying time is 24 hours;

s5: dividing the powder into 3 equal parts, adding the first equal part into the round particles to coat a layer of powder on the surfaces of the round particles, and after the first layer of powder is coated, drying the powder in a dryer, wherein the temperature of the dryer is set to be 30-45 ℃, and the drying time is 2-6 hours;

after the first layer of powder is dried, spraying a layer of water mist on the surface of the first layer of powder, then adding a second equal part of powder, uniformly wrapping the second equal part of powder on the surface of the first layer of powder, and drying in a dryer, wherein the drying setting temperature is 25-35 ℃, and the drying time is 2-6 hours;

after the second layer of powder is dried, spraying a layer of water mist on the surface of the third layer of powder, then adding a third equal part of powder, uniformly coating the third equal part of powder on the surface of the second layer of powder, and drying in a dryer at the set temperature of 30-45 ℃ for 2-6 hours.

The preparation method of the traditional Chinese medicine extractant comprises the following steps:

s1: weighing; radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, lemon juice, Mel, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae;

s2: adding radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae into a rectifying tower, rectifying for 4-10 hr, and collecting distillate;

s3: adding the rectification liquid obtained in the step S2 into a concentration tank for concentration to obtain a concentrated material;

s4: step S3, after the concentrated material is obtained, adding lemon juice and honey into the concentrated material, and uniformly stirring to obtain a traditional Chinese medicine ointment;

s5: transferring the Chinese medicinal paste obtained in step S4 into a dryer, and drying to obtain powdered Chinese medicinal extract.

Example 4:

a probiotics-based antibiotic-free eel feed,

the adhesive comprises the following components in parts by weight:

35 parts of fish meal; 32 parts of starch; 6 parts of vitamin; 2 parts of a mineral mixture; 15 parts of a traditional Chinese medicine extracting agent; 2 parts of bifidobacterium; 3 parts of lactic acid bacteria; 5 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

7 parts of kudzuvine root, 9 parts of astragalus root, 15 parts of suberect spatholobus stem, 15 parts of tangerine peel, 10 parts of cassia bark, 9 parts of lemon juice, 10 parts of honey, 5 parts of chicken's gizzard-membrane, 8 parts of saffron and 10 parts of biond magnolia flower.

A preparation method of probiotics-based eel antibiotic-free feed comprises the following steps:

s1: weighing the following components in parts by weight, namely fish meal; starch; a vitamin; a mineral mixture; a Chinese medicine extracting agent; a bifidobacterium; lactic acid bacteria; soy isoflavones;

s2: fully mixing the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and a forming agent into the materials, fully dissolving the materials into paste, adding the paste into a granulator to prepare round granules, and drying the round granules by using a dryer, wherein the set temperature of the dryer is 29 ℃, and the drying time is 30 hours;

s5: dividing the powder into 3 equal parts, adding the first equal part into the round particles to coat a layer of powder on the surfaces of the round particles, and after the first layer of powder is coated, drying the powder in a dryer, wherein the temperature of the dryer is set to be 30-45 ℃, and the drying time is 2-6 hours;

after the first layer of powder is dried, spraying a layer of water mist on the surface of the first layer of powder, then adding a second equal part of powder, uniformly wrapping the second equal part of powder on the surface of the first layer of powder, and drying in a dryer, wherein the drying setting temperature is 25-35 ℃, and the drying time is 2-6 hours;

after the second layer of powder is dried, spraying a layer of water mist on the surface of the third layer of powder, then adding a third equal part of powder, uniformly coating the third equal part of powder on the surface of the second layer of powder, and drying in a dryer at the set temperature of 30-45 ℃ for 2-6 hours.

The preparation method of the traditional Chinese medicine extractant comprises the following steps:

s1: weighing; radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, lemon juice, Mel, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae;

s2: adding radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae into a rectifying tower, rectifying for 4-10 hr, and collecting distillate;

s3: adding the rectification liquid obtained in the step S2 into a concentration tank for concentration to obtain a concentrated material;

s4: step S3, after the concentrated material is obtained, adding lemon juice and honey into the concentrated material, and uniformly stirring to obtain a traditional Chinese medicine ointment;

s5: transferring the Chinese medicinal paste obtained in step S4 into a dryer, and drying to obtain powdered Chinese medicinal extract.

Example 5

A probiotics-based antibiotic-free eel feed,

the adhesive comprises the following components in parts by weight:

40 parts of fish meal; 38 parts of starch; 8 parts of vitamin; 4 parts of a mineral mixture; 25 parts of a traditional Chinese medicine extracting agent; 3 parts of bifidobacterium; 4 parts of lactic acid bacteria; 10 parts of soybean isoflavone;

the traditional Chinese medicine extractant comprises the following components in parts by weight:

9 parts of kudzuvine root, 10 parts of astragalus root, 20 parts of suberect spatholobus stem, 26 parts of tangerine peel, 15 parts of cassia bark, 12 parts of lemon juice, 13 parts of honey, 7 parts of chicken's gizzard-membrane, 15 parts of saffron and 18 parts of biond magnolia flower.

A preparation method of probiotics-based eel antibiotic-free feed comprises the following steps:

s1: weighing the following components in parts by weight, namely fish meal; starch; a vitamin; a mineral mixture; a Chinese medicine extracting agent; a bifidobacterium; lactic acid bacteria; soy isoflavones;

s2: fully mixing the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and a forming agent into the materials, fully dissolving the materials into paste, adding the paste into a granulator to prepare round granules, and drying the round granules by using a dryer, wherein the temperature of the dryer is set to be 30 ℃, and the drying time is 30 hours;

s5: dividing the powder into 3 equal parts, adding the first equal part into the round particles to coat a layer of powder on the surfaces of the round particles, and after the first layer of powder is coated, drying the powder in a dryer, wherein the temperature of the dryer is set to be 30-45 ℃, and the drying time is 2-6 hours;

after the first layer of powder is dried, spraying a layer of water mist on the surface of the first layer of powder, then adding a second equal part of powder, uniformly wrapping the second equal part of powder on the surface of the first layer of powder, and drying in a dryer, wherein the drying setting temperature is 25-35 ℃, and the drying time is 2-6 hours;

after the second layer of powder is dried, spraying a layer of water mist on the surface of the third layer of powder, then adding a third equal part of powder, uniformly coating the third equal part of powder on the surface of the second layer of powder, and drying in a dryer at the set temperature of 30-45 ℃ for 2-6 hours.

The preparation method of the traditional Chinese medicine extractant comprises the following steps:

s1: weighing; radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, lemon juice, Mel, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae;

s2: adding radix Puerariae, radix astragali, caulis Spatholobi, pericarpium Citri Tangerinae, cortex Cinnamomi, endothelium corneum Gigeriae Galli, stigma croci Sativi, and flos Magnoliae into a rectifying tower, rectifying for 4-10 hr, and collecting distillate;

s3: adding the rectification liquid obtained in the step S2 into a concentration tank for concentration to obtain a concentrated material;

s4: step S3, after the concentrated material is obtained, adding lemon juice and honey into the concentrated material, and uniformly stirring to obtain a traditional Chinese medicine ointment;

s5: transferring the Chinese medicinal paste obtained in step S4 into a dryer, and drying to obtain powdered Chinese medicinal extract.

Comparative example 1:

a nonreactive feed for eel is prepared from eel,

the adhesive comprises the following components in parts by weight:

32 parts of fish meal; 35 parts of starch; 7 parts of vitamin; 3 parts of a mineral mixture; 2 parts of bifidobacterium; 3 parts of lactic acid bacteria; 5 parts of soybean isoflavone;

a preparation method of probiotics-based eel antibiotic-free feed comprises the following steps:

s1: weighing the following components in parts by weight, namely fish meal; starch; a vitamin; a mineral mixture; a bifidobacterium; lactic acid bacteria; soy isoflavones;

s2: fully mixing the fish meal, the traditional Chinese medicine extractant, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and a forming agent into the materials, fully dissolving the materials into paste, adding the paste into a granulator to prepare round granules, and drying the round granules by using a dryer, wherein the set temperature of the dryer is 29 ℃, and the drying time is 30 hours;

s5: dividing the powder into 3 equal parts, adding the first equal part into the round particles to coat a layer of powder on the surfaces of the round particles, and after the first layer of powder is coated, drying the powder in a dryer, wherein the temperature of the dryer is set to be 30-45 ℃, and the drying time is 2-6 hours;

after the first layer of powder is dried, spraying a layer of water mist on the surface of the first layer of powder, then adding a second equal part of powder, uniformly wrapping the second equal part of powder on the surface of the first layer of powder, and drying in a dryer, wherein the drying setting temperature is 25-35 ℃, and the drying time is 2-6 hours;

after the second layer of powder is dried, spraying a layer of water mist on the surface of the third layer of powder, then adding a third equal part of powder, uniformly coating the third equal part of powder on the surface of the second layer of powder, and drying in a dryer at the set temperature of 30-45 ℃ for 2-6 hours.

Comparative example 2:

a nonreactive feed for eel is prepared from eel,

the adhesive comprises the following components in parts by weight:

50 parts of fish meal; 25 parts of starch; 12 parts of vitamin; 1 part of mineral mixture; 1 part of bifidobacterium; 1 part of lactic acid bacteria; 2 parts of soybean isoflavone;

a preparation method of probiotics-based eel antibiotic-free feed comprises the following steps:

s1: weighing the following components in parts by weight, namely fish meal; starch; a vitamin; a mineral mixture; a Chinese medicine extracting agent; a bifidobacterium; lactic acid bacteria; soy isoflavones;

s2: fully mixing the fish meal, the starch and the soybean isoflavone weighed in the step S1 according to the weight parts to obtain powder;

s3: fully mixing the mixture of the bifidobacteria, the lactic acid bacteria, the vitamins and the minerals obtained in the step S1 according to the parts by weight to obtain a material:

s4: adding deionized water and a forming agent into the materials, fully dissolving the materials into paste, adding the paste into a granulator to prepare round granules, and drying the round granules by using a dryer, wherein the temperature of the dryer is set to be 30 ℃, and the drying time is 24 hours;

s5: and (3) directly adding the powder into the material in the S4, and uniformly coating a layer of powder.

Experimental data

The non-resistant eel feed obtained in the experimental examples 1 to 5 and the non-resistant eel feed obtained in the comparative examples 1 and 2 are respectively put into different culture ponds for feeding, the number of eels in the culture ponds is 100, the sizes of eels are equal, after the eels are fed for a period of time, the survival rate, the morbidity and the weight increase of eels in each culture pond are observed, and the specific results are shown in the following table:

	number of onset of disease	Probability of onset	Number of survivors	Probability of survival	Rate of body weight gain
						Experimental example 1	10	10％	90	90％	19％
Experimental example 2	12	12％	88	88％	21％
						Experimental example 3	7	7％	93	93％	25％
Experimental example 4	9	9％	91	91％	20％
						Experimental example 5	8	8％	92	92％	18％
Comparative example 1	19	19％	81	81％	8％
						Comparative example 2	25	25％	75	75％	5％

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.