阅读说明：本技术 标记物品和验证方法 (Marked article and authentication method ) 是由 M·普杜 G·米克尤特斯 于 2019-07-09 设计创作，主要内容包括：本发明提供了用于将核酸示踪剂稳定地固定到产品和物体的表面上的方法。这种方法用于识别和认证标记物体或产品。本发明进一步提供了特定的涂覆制品和它们在产品验证中的用途；制造这样的涂覆制品的方法,验证所述涂覆制品的方法,量化所述涂覆制品掺混的方法,和适合于这样的验证和量化方法的产品。(The present invention provides a method for stably immobilizing a nucleic acid tracer on the surface of products and objects. This method is used to identify and authenticate a marked object or product. The invention further provides specific coated articles and their use in product authentication; methods of making such coated articles, methods of authenticating the coated articles, methods of quantifying blending of the coated articles, and products suitable for such authentication and quantification methods.)

1. A marked article (1) comprising a coating (2) comprising a tracer (3) in a matrix (4), wherein:

the coating (2)

On/applied to the surface (11) of the article (1), and

-completely covering the surface (11) of the article (1), or covering at least one macroscopic surface area (12) of the article, or covering a plurality of microscopic surface areas (13) of the article;

-comprises a plurality of said tracers (3) embedded in said matrix (4);

the tracer (3)

An oligonucleotide comprising a nucleic acid, wherein the oligonucleotide is capable of hybridizing to a nucleic acid,

no covalent bond with the article (1);

the substrate (4)

Is a silica-matrix, and

has a non-porous structure, in particular a non-porous structure, thereby sealing the tracer (3).

2. The marked article according to claim 1, wherein the coating (2) is additionally located in a plurality, preferably more than 100, of microscopic indentations (14) present in the surface (11) of the article (1).

3. The tagged article of claim 1 or 2, wherein the tracer (3)

Consists of free nucleic acids (31); or

Contains or consists of nucleic acids encapsulated within particles (32); or

Containing or consisting of nucleic acids attached to the surface of the particle (33); or

Contains or consists of nucleic acids attached to the surface of the particle, said nucleic acids being encapsulated within the particle (34).

4. The marker article according to any one of the preceding claims, wherein said nucleic acid is selected from the group consisting of naturally occurring nucleic acids and synthetic nucleic acids.

5. The tagged article according to any one of the preceding claims, wherein the substrate (4) is obtained from a precursor; the precursor is selected from the following:

a metal-alkoxylate of formula (I):

M^IV(OR)₄(I) wherein

M represents Si optionally doped with Ti, Zr, Al, and

r independently of one another represents C_1-4An alkyl group;

a functional silane of formula (II):

R’Si(OR)₃(II) in which

R independently of one another represents C_1-4Alkyl, and

r' represents an organofunctional group selected from: c_1-4Aminoalkyl radical, C_1-4Epoxyalkyl, C_1-4Vinyl-alkyl, C_1-4(meth) acryloyloxyalkyl, C_1-4Isocyano-alkyl, C_1-4Mercaptoalkyl and C_1-20An alkyl group; and

a polymeric silane of formula (III):

R”Si(OR)₃(III) in which

R independently of one another represents C_1-4Alkyl, and

r "represents a polymer chain selected from: PEG, polyethylene, polypropylene, PVC, polystyrene, polyurethane.

6. The tagged article of any one of the preceding claims, wherein the article comprises a surface,

is not a silicate; and/or

Inert to aqueous fluoride solutions.

7. The tagged article of any one of the preceding claims, selected from the group consisting of

Luxury;

a fiber or fabric;

articles of clothing and accessories;

an artwork;

an industrial component;

automobile parts;

an electronic device;

paper;

cash or valuables;

-a packaging material;

tobacco;

explosives and weapons;

wood;

pharmaceutical and cosmetic products; and

food and animal feed.

8. Marking article according to any one of the preceding claims, wherein the surface (11) of the article

Having a smooth structure characterized by an arithmetic mean surface roughness lower than 0.8 μm; or

Has a rough structure characterized by an arithmetic mean surface roughness higher than 0.8 μm.

9. A kit of parts, in particular a marking kit for coded identification of a selected article, the kit comprising:

a selected number of containers; which comprises the following steps:

-a first set of containers, each containing a specific and defined suspension or solution of the tracer (3) as defined in claim 1;

a further container comprising a solution of the molecular precursor (5) as defined in claim 5, in particular the molecular precursor (5) as defined in claim 5, dispersed in an alcohol;

an optional further container comprising a solution of catalyst (7);

an optional further container comprising a fluoride buffer solution (6); and

optional means for applying the suspension/solution contained in the container to the selected item to be identified.

10. A verification method for a marked article (1) according to any one of claims 1-8, the method comprising the steps of:

e) providing the tagged item and a fluoride buffer solution;

f) contacting said marked article with said fluoride buffer solution, thereby removing at least a portion of said coating;

g) analyzing the nucleic acid sequence of the composition thus obtained;

h) comparing the obtained nucleic acid sequence with the nucleic acid sequence originally introduced into the tagged article.

11. A quantification method for a marked article (1) according to claims 1-8, the method comprising the steps of:

e) providing the tagged item and a fluoride buffer solution;

f) contacting said marked article with said fluoride buffer solution, thereby removing a portion of said coating;

g) analyzing the nucleic acid sequence of the composition thus obtained;

h) comparing the concentration of the obtained nucleic acid sequence with the concentration of the nucleic acid sequence originally introduced into the article.

12. The method of claim 10 or 11, wherein:

the fluoride buffer solution contains a fluoride salt and hydrofluoric acid; and/or

The contacting comprises the steps of: wiping the surface with a cotton swab, immersing the article in a fluoride solution, or rinsing the surface with a fluoride solution; and/or

The analysis is selected from qPCR, sequence specific isothermal amplification, sequencing.

13. A method of manufacturing a marked article (1), the method comprising the steps of:

a) providing an article (1'), one or more tracers (3), a solution (5) comprising a molecular precursor and optionally a catalyst solution (7); and

b) exposing the surface of the article to the one or more tracers; and

c) coating said article with said molecular precursor solution (5) and optionally said catalyst solution (7); and

d) optionally, cleaning the coated article thus obtained.

14. A method of manufacturing a marked article according to claim 13 wherein:

the tracer(s) (3) are selected from DNA of 50-200 nucleotides in length; and/or

-said solution (5) is selected from metal alkoxides, functional silanes and polymeric silanes, each as defined in claim 5; and/or

Said exposing step b) is selected from spraying, immersing said article and painting; and/or

Said coating step c) is selected from spraying, immersing said article and painting; and/or

The cleaning step d) is selected from washing the article with a solvent, immersing the article in a solvent, mechanical cleaning and polishing.

15. Use of a silica coating for immobilizing nucleic acids on the surface of an article, characterized in that no bond is formed between the nucleic acids and the article and no bond is formed between the nucleic acids and the coating.